CN107334008A - Fruit beverage containing phosphatidylserine and preparation method thereof - Google Patents
Fruit beverage containing phosphatidylserine and preparation method thereof Download PDFInfo
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- CN107334008A CN107334008A CN201710446292.5A CN201710446292A CN107334008A CN 107334008 A CN107334008 A CN 107334008A CN 201710446292 A CN201710446292 A CN 201710446292A CN 107334008 A CN107334008 A CN 107334008A
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- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 title claims abstract description 36
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 235000013361 beverage Nutrition 0.000 title claims abstract description 35
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 239000000203 mixture Substances 0.000 claims abstract description 43
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000010438 heat treatment Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 235000015203 fruit juice Nutrition 0.000 claims abstract description 14
- 229920001214 Polysorbate 60 Polymers 0.000 claims abstract description 13
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 12
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000052 vinegar Substances 0.000 claims abstract description 12
- 235000021419 vinegar Nutrition 0.000 claims abstract description 12
- 239000000811 xylitol Substances 0.000 claims abstract description 12
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 12
- 229960002675 xylitol Drugs 0.000 claims abstract description 12
- 235000010447 xylitol Nutrition 0.000 claims abstract description 12
- 102000011420 Phospholipase D Human genes 0.000 claims abstract description 10
- 108090000553 Phospholipase D Proteins 0.000 claims abstract description 10
- 239000003381 stabilizer Substances 0.000 claims abstract description 8
- 235000011430 Malus pumila Nutrition 0.000 claims abstract description 5
- 235000015103 Malus silvestris Nutrition 0.000 claims abstract description 5
- 235000015165 citric acid Nutrition 0.000 claims abstract description 5
- 238000000638 solvent extraction Methods 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 20
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 12
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- 235000010413 sodium alginate Nutrition 0.000 claims description 5
- 239000000661 sodium alginate Substances 0.000 claims description 5
- 229940005550 sodium alginate Drugs 0.000 claims description 5
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 4
- 241000206575 Chondrus crispus Species 0.000 claims description 3
- 235000019987 cider Nutrition 0.000 claims description 3
- 229920001285 xanthan gum Polymers 0.000 claims description 3
- 244000241235 Citrullus lanatus Species 0.000 claims description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims description 2
- 244000288157 Passiflora edulis Species 0.000 claims description 2
- 235000000370 Passiflora edulis Nutrition 0.000 claims description 2
- 235000004400 serine Nutrition 0.000 abstract description 10
- 150000003355 serines Chemical class 0.000 abstract 1
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 37
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 35
- 239000003921 oil Substances 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 4
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 3
- 102000015439 Phospholipases Human genes 0.000 description 3
- 108010064785 Phospholipases Proteins 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 229940090949 docosahexaenoic acid Drugs 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 230000001256 tonic effect Effects 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 230000003925 brain function Effects 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- 125000001095 phosphatidyl group Chemical group 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of fruit beverage containing phosphatidylserine and preparation method thereof, the preparation method includes:Mixture A is obtained after DHA algal oil, polyoxyethylene sorbitan monoleate and water are mixed;L serines, phospholipase D and cushioning liquid are mixed, obtain mixture B after heating;Through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;After phosphatidylserine obtained above, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water are mixed the fruit beverage is obtained through vacuum outgas;DHA and PS is rich in obtained fruit beverage, passes through DHA and PS synergy, it is possible to increase DHA stability, promotes human body to absorb it.
Description
Technical field
The present invention relates to field of food preparation, in particular it relates to fruit beverage containing phosphatidylserine and preparation method thereof.
Background technology
Phosphatidylserine (Phosphatidylserine, PS) be mammalian cell membrane phosphatide important composition into
Point, especially in brain tissue, PS can successfully pass through blood-brain barrier, and a few minutes after being absorbed can enter intracerebral, its
It is closely related with the brain function such as cognition, mood, study, memory, it is depressed to be applied to improve Alzheimer disease, treatment at present
Disease, alleviate stress and physical fatigue etc..Docosahexaenoic acid (docosahexaenoicacid, DHA) is in brain tonic
Intelligence development and protection eyesight etc. have remarkable effect, and free DHA is unstable, oxidizable, and independent take can increase stomach and intestine
Burden.How DHA and PS to be synthesized, and are applied in food manufacture field, further to improve human body to DHA's and PS
The problem of absorption is urgent need to resolve of the present invention.
The content of the invention
It is an object of the invention to provide a kind of fruit beverage containing phosphatidylserine and preparation method thereof, obtained fruit beverage
In be rich in DHA and PS, pass through DHA and PS synergy, it is possible to increase DHA stability, promote human body it is absorbed.
To achieve these goals, the invention provides a kind of preparation method of the fruit beverage containing phosphatidylserine, institute
Stating preparation method includes:
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water;
(2) Serine, phospholipase D and cushioning liquid mixed, obtain mixture B after heating;
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;
(4) phosphatidylserine, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water that step (3) obtains are mixed
After conjunction the fruit beverage is obtained through vacuum outgas.
Present invention also offers a kind of fruit beverage containing phosphatidylserine, the fruit beverage is by above-mentioned preparation method system
.
Pass through above-mentioned technical proposal, the invention provides a kind of preparation method of the fruit beverage containing phosphatidylserine, institute
Stating preparation method includes:Mixture A is obtained after DHA algal oil, polyoxyethylene sorbitan monoleate and water are mixed;By Serine, phospholipase D and
Mixture B is obtained after cushioning liquid mixing, heating;Through solvent extraction after mixture A and mixture B is mixed, obtain being rich in DHA
Phosphatidylserine;By phosphatidylserine obtained above, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water
After mixing the fruit beverage is obtained through vacuum outgas;It is oxidizable because free DHA is unstable, and independent take can increase stomach
Intestines are born, and by the synthesis to DHA and PS, are increased DHA stability, by both synergies, can be promoted human body pair
The absorption of DHA and PS in fruit beverage so that obtained fruit beverage, which possesses, alleviates stress and physical fatigue, brain tonic and intelligence development
Function.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Embodiment
The embodiment of the present invention is described in detail below.It is it should be appreciated that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The end points of disclosed scope and any value are not limited to the accurate scope or value herein, these scopes or
Value should be understood to comprising the value close to these scopes or value.For number range, between the endpoint value of each scope, respectively
It can be combined with each other between the endpoint value of individual scope and single point value, and individually between point value and obtain one or more
New number range, these number ranges should be considered as specific open herein.
The invention provides a kind of preparation method of the fruit beverage containing phosphatidylserine, the preparation method includes:
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water;
(2) Serine, phospholipase D and cushioning liquid mixed, obtain mixture B after heating;
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;
(4) phosphatidylserine, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water that step (3) obtains are mixed
After conjunction the fruit beverage is obtained through vacuum outgas.
In a kind of preferred embodiment of the present invention, in order to further improve human body to DHA's in fruit beverage and PS
Absorb, relative to the water of 100 parts by weight, relative to the water of 100 parts by weight, the dosage of the DHA algal oil is 0.2-0.6 weight
Part, the dosage of the polyoxyethylene sorbitan monoleate is 2-8 parts by weight, relative to the cushioning liquid of 100 parts by weight, the use of the Serine
Measure as 10-20 parts by weight, the dosage of the phospholipase D is 0.5-5.5 parts by weight;
Relative to the fruit juice of 100 parts by weight, the dosage of the phosphatidylserine is 1-3 parts by weight, the citric acid
Dosage is 3-8 parts by weight, and the dosage of the xylitol is 2-7 parts by weight, and the dosage of the apple vinegar is 0.5-2.5 parts by weight,
The dosage of the stabilizer is 0.1-0.4 parts by weight, and the dosage of the water is 20-40 parts by weight.
In a kind of preferred embodiment of the present invention, in order to further improve DHA and PS combined coefficient, step
(3) mixture A and mixture B is 1 according to volume ratio in:20-40 is mixed.
In a kind of preferred embodiment of the present invention, in order that phosphatidylserine purity is higher made from obtaining, institute
State cushioning liquid to be made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1:2-5.
In a kind of preferred embodiment of the present invention, in order that phosphatidylserine purity is higher made from obtaining, step
Suddenly the temperature of heating is 40-60 DEG C in (2), and the time of heating is 4-8h.
In a kind of preferred embodiment of the present invention, in order to obtain the higher phosphatidyl silk ammonia rich in DHA of purity
Acid, the solvent include ethanol, ether and water;And relative to the water of 100 parts by weight, the dosage of the ethanol is 20-40 weight
Part, the dosage of the ether is 10-20 parts by weight.
In a kind of preferred embodiment of the present invention, in order that obtaining fruit beverage has various tastes to meet different crowd
Demand, the fruit juice be cider, Passion Fruit Juice, watermelon juice or cider in one kind.
In a kind of preferred embodiment of the present invention, in order that can preferably be mixed between obtaining raw material, it is described steady
Agent is determined for the one or more in xanthans, carragheen and sodium alginate.
In a kind of preferred embodiment of the present invention, in order that can preferably be mixed between obtaining raw material, it is described true
The temperature of sky degassing is 25-35 DEG C, and the vacuum of vacuum outgas is 0.18-0.22Mpa, and the time of vacuum outgas is 30-50s.
Present invention also offers a kind of fruit beverage containing phosphatidylserine, the fruit beverage is by above-mentioned preparation method system
.
The present invention will be described in detail by way of examples below.In following examples, DHA algal oil is the azure life in Qingdao
The commercially available product that thing Group Co., Ltd provides;Polyoxyethylene sorbitan monoleate is the commercially available product that Science and Technology Ltd. of Beijing Lingbao City provides;L- silks
Propylhomoserin is the commercially available product that three outstanding Bioisystech Co., Ltd of Shanghai provides;Phospholipase D is the city that moral fills in the offer of (China) company admittedly
Sell product.
Embodiment 1
Mixture A is obtained after 0.2gDHA algae oils, 2g polyoxyethylene sorbitan monoleates and 100g water are mixed;By 10gL- serines,
(cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 0.5g phospholipase Ds and 100g cushioning liquid:2)
Mixture B is obtained after mixing, heating (temperature of heating is 40 DEG C, and the time of heating is 4h);Mixture A and mixture B are mixed
Close that (mixture A and mixture B are 1 according to volume ratio:20 are mixed) by solvent (solvent by 20g ethanol, 10g ether and
100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA;By phosphatidylserine 1g obtained above, fruit juice
Through vacuum outgas (vacuum outgas after 100g, citric acid 3g, xylitol 2g, apple vinegar 0.5g, xanthans 0.1g and water 20g mixing
Temperature be 25 DEG C, the vacuum of vacuum outgas is 0.18Mpa, and the time of vacuum outgas is 30s) obtain the fruit beverage A1.
Embodiment 2
Mixture A is obtained after 0.6gDHA algae oils, 8g polyoxyethylene sorbitan monoleates and 100g water are mixed;By 20gL- serines,
(cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 5.5g phospholipase Ds and 100g cushioning liquid:5)
Mixture B is obtained after mixing, heating (temperature of heating is 60 DEG C, and the time of heating is 8h);Mixture A and mixture B are mixed
Close that (mixture A and mixture B are 1 according to volume ratio:40 are mixed) by solvent (solvent by 40g ethanol, 20g ether and
100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA;By phosphatidylserine 3g obtained above, fruit juice
Through vacuum outgas (vacuum outgas after 100g, citric acid 8g, xylitol 7g, apple vinegar 2.5g, carragheen 0.4g and water 40g mixing
Temperature be 35 DEG C, the vacuum of vacuum outgas is 0.22Mpa, and the time of vacuum outgas is 50s) obtain the fruit beverage A2.
Embodiment 3
Mixture A is obtained after 0.4gDHA algae oils, 5g polyoxyethylene sorbitan monoleates and 100g water are mixed;By 15gL- serines,
(cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 3.5g phospholipase Ds and 100g cushioning liquid:3)
Mixture B is obtained after mixing, heating (temperature of heating is 50 DEG C, and the time of heating is 6h);Mixture A and mixture B are mixed
Close that (mixture A and mixture B are 1 according to volume ratio:30 are mixed) by solvent (solvent by 30g ethanol, 15g ether and
100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA;By phosphatidylserine 2g obtained above, fruit juice
Through vacuum outgas, (vacuum takes off after 100g, citric acid 5g, xylitol 5g, apple vinegar 1.5g, sodium alginate 0.2g and water 30g mixing
The temperature of gas is 35 DEG C, and the vacuum of vacuum outgas is 0.22Mpa, and the time of vacuum outgas is 50s) obtain the fruit beverage
A3。
Comparative example 1
It is prepared by the method according to embodiment 3, unlike, relative to 100g water, the dosage of the DHA algal oil is
0.1g, the dosage of the polyoxyethylene sorbitan monoleate is 1g, and relative to 100g cushioning liquid, the dosage of the Serine is 7g, institute
The dosage for stating phospholipase D is 0.2g;Relative to 100g fruit juice, the dosage of the phosphatidylserine is 0.5g, the lemon
The dosage of acid is 2g, and the dosage of the xylitol is 1g, and the dosage of the apple vinegar is 0.3g, and the dosage of the sodium alginate is
0.05g, the dosage of the water is 15g, obtains the fruit beverage D1.
Comparative example 2
It is prepared by the method according to embodiment 3, unlike, relative to 100g water, the dosage of the DHA algal oil is
0.8g, the dosage of the polyoxyethylene sorbitan monoleate is 10g, and relative to 100g cushioning liquid, the dosage of the Serine is 25g,
The dosage of the phospholipase D is 7g;Relative to 100g fruit juice, the dosage of the phosphatidylserine is 5g, the citric acid
Dosage be 10g, the dosage of the xylitol is 8g, and the dosage of the apple vinegar is 3g, and the dosage of the sodium alginate is
0.5g, the dosage of the water is 50g, obtains the fruit beverage D2.
Table 1
Embodiment is numbered | PS synthetic ratios (%) | DHA content (g/L) in fruit beverage |
Embodiment 1 | 97 | 2.7 |
Embodiment 2 | 95 | 2.4 |
Embodiment 3 | 96 | 2.3 |
Comparative example 1 | 81 | 1.4 |
Comparative example 2 | 84 | 1.2 |
It can be seen that by above table data in preparation method within the scope of the present invention, PS synthetic ratio is higher, reaches
To 95-97%, DHA content is higher in obtained fruit beverage A1-A3, and in preparation method outside the scope of the present invention, PS's
Synthetic ratio is relatively low, and DHA content is relatively low in obtained fruit beverage A1-A3.In obtained fruit beverage rich in DHA and
PS, pass through DHA and PS synergy, it is possible to increase DHA stability, promote human body to absorb it.
The preferred embodiment of the present invention described in detail above, still, the present invention are not limited in above-mentioned embodiment
Detail, in the range of the technology design of the present invention, a variety of simple variants can be carried out to technical scheme, this
A little simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (10)
1. a kind of preparation method of the fruit beverage containing phosphatidylserine, it is characterised in that the preparation method includes:
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water;
(2) Serine, phospholipase D and cushioning liquid mixed, obtain mixture B after heating;
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;
(4) after the phosphatidylserine, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and the water mixing that obtain step (3)
The fruit beverage is obtained through vacuum outgas.
2. preparation method according to claim 1, wherein, relative to the water of 100 parts by weight, the dosage of the DHA algal oil
For 0.2-0.6 parts by weight, the dosage of the polyoxyethylene sorbitan monoleate is 2-8 parts by weight, relative to the cushioning liquid of 100 parts by weight, institute
The dosage for stating Serine is 10-20 parts by weight, and the dosage of the phospholipase D is 0.5-5.5 parts by weight;
Relative to the fruit juice of 100 parts by weight, the dosage of the phosphatidylserine is 1-3 parts by weight, the dosage of the citric acid
For 3-8 parts by weight, the dosage of the xylitol is 2-7 parts by weight, and the dosage of the apple vinegar is 0.5-2.5 parts by weight, described
The dosage of stabilizer is 0.1-0.4 parts by weight, and the dosage of the water is 20-40 parts by weight.
3. preparation method according to claim 1 or 2, wherein, mixture A and mixture B are according to volume ratio in step (3)
For 1:20-40 is mixed.
4. preparation method according to claim 3, wherein, the cushioning liquid is made up of chloroform and ethanol, and chloroform and
The volume ratio of ethanol is 1:2-5.
5. preparation method according to claim 4, wherein, the temperature of heating is 40-60 DEG C in step (2), heating when
Between be 4-8h.
6. preparation method according to claim 5, wherein, the solvent includes ethanol, ether and water;
And relative to the water of 100 parts by weight, the dosage of the ethanol is 20-40 parts by weight, and the dosage of the ether is 10-20 weights
Measure part.
7. preparation method according to claim 1, wherein, the fruit juice is cider, Passion Fruit Juice, watermelon juice or apple
One kind in juice.
8. preparation method according to claim 1, wherein, the stabilizer is in xanthans, carragheen and sodium alginate
One or more.
9. preparation method according to claim 1, wherein, the temperature of the vacuum outgas is 25-35 DEG C, vacuum outgas
Vacuum is 0.18-0.22Mpa, and the time of vacuum outgas is 30-50s.
10. a kind of fruit beverage containing phosphatidylserine, it is characterised in that the fruit beverage is by any one in claim 1-9
Preparation method described in is made.
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