CN107334008A - Fruit beverage containing phosphatidylserine and preparation method thereof - Google Patents

Fruit beverage containing phosphatidylserine and preparation method thereof Download PDF

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Publication number
CN107334008A
CN107334008A CN201710446292.5A CN201710446292A CN107334008A CN 107334008 A CN107334008 A CN 107334008A CN 201710446292 A CN201710446292 A CN 201710446292A CN 107334008 A CN107334008 A CN 107334008A
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weight
dosage
parts
preparation
mixture
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黄宁民
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Wuhu Fumin Pharmaceutical Ltd By Share Ltd
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Wuhu Fumin Pharmaceutical Ltd By Share Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12Fatty acids or derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/175Amino acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses a kind of fruit beverage containing phosphatidylserine and preparation method thereof, the preparation method includes:Mixture A is obtained after DHA algal oil, polyoxyethylene sorbitan monoleate and water are mixed;L serines, phospholipase D and cushioning liquid are mixed, obtain mixture B after heating;Through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;After phosphatidylserine obtained above, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water are mixed the fruit beverage is obtained through vacuum outgas;DHA and PS is rich in obtained fruit beverage, passes through DHA and PS synergy, it is possible to increase DHA stability, promotes human body to absorb it.

Description

Fruit beverage containing phosphatidylserine and preparation method thereof
Technical field
The present invention relates to field of food preparation, in particular it relates to fruit beverage containing phosphatidylserine and preparation method thereof.
Background technology
Phosphatidylserine (Phosphatidylserine, PS) be mammalian cell membrane phosphatide important composition into Point, especially in brain tissue, PS can successfully pass through blood-brain barrier, and a few minutes after being absorbed can enter intracerebral, its It is closely related with the brain function such as cognition, mood, study, memory, it is depressed to be applied to improve Alzheimer disease, treatment at present Disease, alleviate stress and physical fatigue etc..Docosahexaenoic acid (docosahexaenoicacid, DHA) is in brain tonic Intelligence development and protection eyesight etc. have remarkable effect, and free DHA is unstable, oxidizable, and independent take can increase stomach and intestine Burden.How DHA and PS to be synthesized, and are applied in food manufacture field, further to improve human body to DHA's and PS The problem of absorption is urgent need to resolve of the present invention.
The content of the invention
It is an object of the invention to provide a kind of fruit beverage containing phosphatidylserine and preparation method thereof, obtained fruit beverage In be rich in DHA and PS, pass through DHA and PS synergy, it is possible to increase DHA stability, promote human body it is absorbed.
To achieve these goals, the invention provides a kind of preparation method of the fruit beverage containing phosphatidylserine, institute Stating preparation method includes:
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water;
(2) Serine, phospholipase D and cushioning liquid mixed, obtain mixture B after heating;
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;
(4) phosphatidylserine, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water that step (3) obtains are mixed After conjunction the fruit beverage is obtained through vacuum outgas.
Present invention also offers a kind of fruit beverage containing phosphatidylserine, the fruit beverage is by above-mentioned preparation method system .
Pass through above-mentioned technical proposal, the invention provides a kind of preparation method of the fruit beverage containing phosphatidylserine, institute Stating preparation method includes:Mixture A is obtained after DHA algal oil, polyoxyethylene sorbitan monoleate and water are mixed;By Serine, phospholipase D and Mixture B is obtained after cushioning liquid mixing, heating;Through solvent extraction after mixture A and mixture B is mixed, obtain being rich in DHA Phosphatidylserine;By phosphatidylserine obtained above, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water After mixing the fruit beverage is obtained through vacuum outgas;It is oxidizable because free DHA is unstable, and independent take can increase stomach Intestines are born, and by the synthesis to DHA and PS, are increased DHA stability, by both synergies, can be promoted human body pair The absorption of DHA and PS in fruit beverage so that obtained fruit beverage, which possesses, alleviates stress and physical fatigue, brain tonic and intelligence development Function.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Embodiment
The embodiment of the present invention is described in detail below.It is it should be appreciated that described herein specific Embodiment is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The end points of disclosed scope and any value are not limited to the accurate scope or value herein, these scopes or Value should be understood to comprising the value close to these scopes or value.For number range, between the endpoint value of each scope, respectively It can be combined with each other between the endpoint value of individual scope and single point value, and individually between point value and obtain one or more New number range, these number ranges should be considered as specific open herein.
The invention provides a kind of preparation method of the fruit beverage containing phosphatidylserine, the preparation method includes:
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water;
(2) Serine, phospholipase D and cushioning liquid mixed, obtain mixture B after heating;
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;
(4) phosphatidylserine, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and water that step (3) obtains are mixed After conjunction the fruit beverage is obtained through vacuum outgas.
In a kind of preferred embodiment of the present invention, in order to further improve human body to DHA's in fruit beverage and PS Absorb, relative to the water of 100 parts by weight, relative to the water of 100 parts by weight, the dosage of the DHA algal oil is 0.2-0.6 weight Part, the dosage of the polyoxyethylene sorbitan monoleate is 2-8 parts by weight, relative to the cushioning liquid of 100 parts by weight, the use of the Serine Measure as 10-20 parts by weight, the dosage of the phospholipase D is 0.5-5.5 parts by weight;
Relative to the fruit juice of 100 parts by weight, the dosage of the phosphatidylserine is 1-3 parts by weight, the citric acid Dosage is 3-8 parts by weight, and the dosage of the xylitol is 2-7 parts by weight, and the dosage of the apple vinegar is 0.5-2.5 parts by weight, The dosage of the stabilizer is 0.1-0.4 parts by weight, and the dosage of the water is 20-40 parts by weight.
In a kind of preferred embodiment of the present invention, in order to further improve DHA and PS combined coefficient, step (3) mixture A and mixture B is 1 according to volume ratio in:20-40 is mixed.
In a kind of preferred embodiment of the present invention, in order that phosphatidylserine purity is higher made from obtaining, institute State cushioning liquid to be made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1:2-5.
In a kind of preferred embodiment of the present invention, in order that phosphatidylserine purity is higher made from obtaining, step Suddenly the temperature of heating is 40-60 DEG C in (2), and the time of heating is 4-8h.
In a kind of preferred embodiment of the present invention, in order to obtain the higher phosphatidyl silk ammonia rich in DHA of purity Acid, the solvent include ethanol, ether and water;And relative to the water of 100 parts by weight, the dosage of the ethanol is 20-40 weight Part, the dosage of the ether is 10-20 parts by weight.
In a kind of preferred embodiment of the present invention, in order that obtaining fruit beverage has various tastes to meet different crowd Demand, the fruit juice be cider, Passion Fruit Juice, watermelon juice or cider in one kind.
In a kind of preferred embodiment of the present invention, in order that can preferably be mixed between obtaining raw material, it is described steady Agent is determined for the one or more in xanthans, carragheen and sodium alginate.
In a kind of preferred embodiment of the present invention, in order that can preferably be mixed between obtaining raw material, it is described true The temperature of sky degassing is 25-35 DEG C, and the vacuum of vacuum outgas is 0.18-0.22Mpa, and the time of vacuum outgas is 30-50s.
Present invention also offers a kind of fruit beverage containing phosphatidylserine, the fruit beverage is by above-mentioned preparation method system .
The present invention will be described in detail by way of examples below.In following examples, DHA algal oil is the azure life in Qingdao The commercially available product that thing Group Co., Ltd provides;Polyoxyethylene sorbitan monoleate is the commercially available product that Science and Technology Ltd. of Beijing Lingbao City provides;L- silks Propylhomoserin is the commercially available product that three outstanding Bioisystech Co., Ltd of Shanghai provides;Phospholipase D is the city that moral fills in the offer of (China) company admittedly Sell product.
Embodiment 1
Mixture A is obtained after 0.2gDHA algae oils, 2g polyoxyethylene sorbitan monoleates and 100g water are mixed;By 10gL- serines, (cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 0.5g phospholipase Ds and 100g cushioning liquid:2) Mixture B is obtained after mixing, heating (temperature of heating is 40 DEG C, and the time of heating is 4h);Mixture A and mixture B are mixed Close that (mixture A and mixture B are 1 according to volume ratio:20 are mixed) by solvent (solvent by 20g ethanol, 10g ether and 100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA;By phosphatidylserine 1g obtained above, fruit juice Through vacuum outgas (vacuum outgas after 100g, citric acid 3g, xylitol 2g, apple vinegar 0.5g, xanthans 0.1g and water 20g mixing Temperature be 25 DEG C, the vacuum of vacuum outgas is 0.18Mpa, and the time of vacuum outgas is 30s) obtain the fruit beverage A1.
Embodiment 2
Mixture A is obtained after 0.6gDHA algae oils, 8g polyoxyethylene sorbitan monoleates and 100g water are mixed;By 20gL- serines, (cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 5.5g phospholipase Ds and 100g cushioning liquid:5) Mixture B is obtained after mixing, heating (temperature of heating is 60 DEG C, and the time of heating is 8h);Mixture A and mixture B are mixed Close that (mixture A and mixture B are 1 according to volume ratio:40 are mixed) by solvent (solvent by 40g ethanol, 20g ether and 100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA;By phosphatidylserine 3g obtained above, fruit juice Through vacuum outgas (vacuum outgas after 100g, citric acid 8g, xylitol 7g, apple vinegar 2.5g, carragheen 0.4g and water 40g mixing Temperature be 35 DEG C, the vacuum of vacuum outgas is 0.22Mpa, and the time of vacuum outgas is 50s) obtain the fruit beverage A2.
Embodiment 3
Mixture A is obtained after 0.4gDHA algae oils, 5g polyoxyethylene sorbitan monoleates and 100g water are mixed;By 15gL- serines, (cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 3.5g phospholipase Ds and 100g cushioning liquid:3) Mixture B is obtained after mixing, heating (temperature of heating is 50 DEG C, and the time of heating is 6h);Mixture A and mixture B are mixed Close that (mixture A and mixture B are 1 according to volume ratio:30 are mixed) by solvent (solvent by 30g ethanol, 15g ether and 100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA;By phosphatidylserine 2g obtained above, fruit juice Through vacuum outgas, (vacuum takes off after 100g, citric acid 5g, xylitol 5g, apple vinegar 1.5g, sodium alginate 0.2g and water 30g mixing The temperature of gas is 35 DEG C, and the vacuum of vacuum outgas is 0.22Mpa, and the time of vacuum outgas is 50s) obtain the fruit beverage A3。
Comparative example 1
It is prepared by the method according to embodiment 3, unlike, relative to 100g water, the dosage of the DHA algal oil is 0.1g, the dosage of the polyoxyethylene sorbitan monoleate is 1g, and relative to 100g cushioning liquid, the dosage of the Serine is 7g, institute The dosage for stating phospholipase D is 0.2g;Relative to 100g fruit juice, the dosage of the phosphatidylserine is 0.5g, the lemon The dosage of acid is 2g, and the dosage of the xylitol is 1g, and the dosage of the apple vinegar is 0.3g, and the dosage of the sodium alginate is 0.05g, the dosage of the water is 15g, obtains the fruit beverage D1.
Comparative example 2
It is prepared by the method according to embodiment 3, unlike, relative to 100g water, the dosage of the DHA algal oil is 0.8g, the dosage of the polyoxyethylene sorbitan monoleate is 10g, and relative to 100g cushioning liquid, the dosage of the Serine is 25g, The dosage of the phospholipase D is 7g;Relative to 100g fruit juice, the dosage of the phosphatidylserine is 5g, the citric acid Dosage be 10g, the dosage of the xylitol is 8g, and the dosage of the apple vinegar is 3g, and the dosage of the sodium alginate is 0.5g, the dosage of the water is 50g, obtains the fruit beverage D2.
Table 1
Embodiment is numbered PS synthetic ratios (%) DHA content (g/L) in fruit beverage
Embodiment 1 97 2.7
Embodiment 2 95 2.4
Embodiment 3 96 2.3
Comparative example 1 81 1.4
Comparative example 2 84 1.2
It can be seen that by above table data in preparation method within the scope of the present invention, PS synthetic ratio is higher, reaches To 95-97%, DHA content is higher in obtained fruit beverage A1-A3, and in preparation method outside the scope of the present invention, PS's Synthetic ratio is relatively low, and DHA content is relatively low in obtained fruit beverage A1-A3.In obtained fruit beverage rich in DHA and PS, pass through DHA and PS synergy, it is possible to increase DHA stability, promote human body to absorb it.
The preferred embodiment of the present invention described in detail above, still, the present invention are not limited in above-mentioned embodiment Detail, in the range of the technology design of the present invention, a variety of simple variants can be carried out to technical scheme, this A little simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned embodiment, in not lance In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally The thought of invention, it should equally be considered as content disclosed in this invention.

Claims (10)

1. a kind of preparation method of the fruit beverage containing phosphatidylserine, it is characterised in that the preparation method includes:
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water;
(2) Serine, phospholipase D and cushioning liquid mixed, obtain mixture B after heating;
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained;
(4) after the phosphatidylserine, fruit juice, citric acid, xylitol, apple vinegar, stabilizer and the water mixing that obtain step (3) The fruit beverage is obtained through vacuum outgas.
2. preparation method according to claim 1, wherein, relative to the water of 100 parts by weight, the dosage of the DHA algal oil For 0.2-0.6 parts by weight, the dosage of the polyoxyethylene sorbitan monoleate is 2-8 parts by weight, relative to the cushioning liquid of 100 parts by weight, institute The dosage for stating Serine is 10-20 parts by weight, and the dosage of the phospholipase D is 0.5-5.5 parts by weight;
Relative to the fruit juice of 100 parts by weight, the dosage of the phosphatidylserine is 1-3 parts by weight, the dosage of the citric acid For 3-8 parts by weight, the dosage of the xylitol is 2-7 parts by weight, and the dosage of the apple vinegar is 0.5-2.5 parts by weight, described The dosage of stabilizer is 0.1-0.4 parts by weight, and the dosage of the water is 20-40 parts by weight.
3. preparation method according to claim 1 or 2, wherein, mixture A and mixture B are according to volume ratio in step (3) For 1:20-40 is mixed.
4. preparation method according to claim 3, wherein, the cushioning liquid is made up of chloroform and ethanol, and chloroform and The volume ratio of ethanol is 1:2-5.
5. preparation method according to claim 4, wherein, the temperature of heating is 40-60 DEG C in step (2), heating when Between be 4-8h.
6. preparation method according to claim 5, wherein, the solvent includes ethanol, ether and water;
And relative to the water of 100 parts by weight, the dosage of the ethanol is 20-40 parts by weight, and the dosage of the ether is 10-20 weights Measure part.
7. preparation method according to claim 1, wherein, the fruit juice is cider, Passion Fruit Juice, watermelon juice or apple One kind in juice.
8. preparation method according to claim 1, wherein, the stabilizer is in xanthans, carragheen and sodium alginate One or more.
9. preparation method according to claim 1, wherein, the temperature of the vacuum outgas is 25-35 DEG C, vacuum outgas Vacuum is 0.18-0.22Mpa, and the time of vacuum outgas is 30-50s.
10. a kind of fruit beverage containing phosphatidylserine, it is characterised in that the fruit beverage is by any one in claim 1-9 Preparation method described in is made.
CN201710446292.5A 2017-06-14 2017-06-14 Fruit beverage containing phosphatidylserine and preparation method thereof Pending CN107334008A (en)

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CN106086097A (en) * 2016-06-08 2016-11-09 芜湖福民生物药业有限公司 The preparation method of Phosphatidylserine
CN106473076A (en) * 2016-10-14 2017-03-08 广东红珊瑚药业有限公司 A kind of composition for improving brain memory and its application

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Application publication date: 20171110