CN107308101B

CN107308101B - Skin tightening composition and application thereof

Info

Publication number: CN107308101B
Application number: CN201710442863.8A
Authority: CN
Inventors: 戴跃锋; 康文术; 何广文; 颜少慰
Original assignee: Hunan Yujia Cosmetics Manufacturing Co ltd
Current assignee: Syoung Cosmetics Manufacturing Co Ltd
Priority date: 2017-06-13
Filing date: 2017-06-13
Publication date: 2020-06-19
Anticipated expiration: 2037-06-13
Also published as: CN107308101A

Abstract

The present invention relates to a skin tightening composition comprising: hyaluronic acid, hydrolyzed collagen, dipalmitoyl hydroxyproline, compound amino acid, decarboxylated peptide, acetylated hexapeptide, plankton extract, lactobacillus/soybean milk fermentation product filtrate, dipotassium glycyrrhizinate, retinol palmitate, VE and hydrolyzed sesame protein. The composition adopts anti-aging vitamins, amino acids, derivatives and peptides, improves an extracellular matrix structure from the endogenous source, adopts dipalmitoyl hydroxyproline, palmitoyl glycine, decarboxylated carnosine, acetylated hexapeptide, sodium hyaluronate, retinol, hydrolyzed collagen, plankton extract, lactobacillus/soybean milk fermentation product filtrate and dipotassium glycyrrhizinate as main components, and aims at comprehensively repairing wrinkles and fine lines appearing in skin relaxation by promoting synthesis of collagen and elastin, inhibiting activity of metalloproteinase, improving skin firmness and elasticity and recovering young skin.

Description

Skin tightening composition and application thereof

Technical Field

The invention relates to the technical field of skin care products, in particular to a skin tightening composition (uniBoost) and application thereof.

Background

Skin care is the most important market segment in the global beauty and personal care industry, and anti-aging products are the focus of market attention. Since ancient times, people have been bitter to seek active substances with anti-aging effects. These applications of substances not only exist in the history of ancient Egypt, but can even be traced back to India and China in ancient times.

The demand of anti-aging products is promoted by the aging of the world, the population tends to age, the consumption population of anti-aging products is increasingly strong, the aging population of countries is not only the problems of America, Europe and Japan, but also the aging population of emerging countries such as China, Thailand and the like.

Skin is aged all the time, the elasticity of the skin is slowly reduced, the skin becomes thinner and thinner, and the skin is more fragile and looser, the skin is full of wrinkles, the skin aging resistance is to prevent the skin from generating the changes of the main functions and organic degeneracy due to the time lapse, and the changes are expressed in the prevention of the wrinkles, dryness, scurf, laxity and color spots.

In recent years, many cosmetics or skin care products have been favored because of their skin-caring and rejuvenating effects. Early on, many animal glandular and serum extracts had significant cosmetic and cosmetology effects, but were less and less advocated. Over the past few years, the cosmetic industry has gained increased understanding of the structure and function of the skin, increasing the ability of the cosmetic industry to determine whether a formulation is beneficial to the skin.

For over 50 years, skin care scientists have been dedicated to removing scars, spots or imperfections on the skin surface by peeling the skin to make it glow with a new skin color, but lack the effect of accelerating cell renewal or exfoliation by daily maintenance or repeated skin peeling to achieve youthful appearance. Aiming at the appearance of skin aging caused by skin aging, such as skin elasticity reduction, collagen denaturation, facial wrinkles, relaxation, senile plaques and the like. Skin tissue damage and photoaging caused by long-term or frequent irradiation and ultraviolet pressing irradiation in sunlight, free radicals and induced oxidation or excessive chain reaction. Previously anti-aging products were limited to consumers over 50 years old, but are currently favored by consumers over 30 years old, and even younger.

How to effectively delay skin aging and relieve skin extensive damage and the like is always highly valued and concerned by the anti-aging medical, biological and cosmetic industries.

Disclosure of Invention

Based on this, it is an object of the present invention to provide a skin tightening composition with anti-aging effect.

The specific technical scheme is as follows:

a skin tightening composition (uniBoost) comprising: hyaluronic acid, hydrolyzed collagen, dipalmitoyl hydroxyproline, compound amino acid, decarboxylated peptide, acetylated hexapeptide, plankton extract, lactobacillus/soybean milk fermentation product filtrate, dipotassium glycyrrhizinate, retinol palmitate, VE, Arabinorhizobium extract, and hydrolyzed sesame protein.

In some embodiments, the skin tightening composition comprises the following raw material components in parts by mass:

in some embodiments, the compound amino acid comprises the following components in percentage by mass:

it is another object of the present invention to provide the above-mentioned skin tightening composition (uniBoost) for use in cosmetics.

In some of these embodiments, the cosmetic comprises a facial cleanser, a toner, a cream, a serum, a cream, a mask, or a color cosmetic.

It is another object of the present invention to provide a cosmetic product comprising the above skin tightening composition.

With the development of socio-economy, many countries including china have entered into an aging social line, and thus anti-aging products are receiving much attention. Anti-aging is the most concerned and most popular beauty care service for women.

Aging, also known as aging, is a stage that must be experienced during the course of a living organism's activities, and generally refers to the progressive process of functional and organic decline of the organism over time after the organism develops and matures under normal conditions. Thus, aging is not a disease, but a physiological phenomenon. The skin is located on the body surface and is one of the most prominent organs in the aging process of the body.

With the increasing living standard of people, people also pay attention to the problem of skin aging after meeting the basic requirements of clothes, eating and housing. The pursuit of the unadorned face by people is absolutely not present, and from ancient times to the present, from the west to the east, from the black to the white, the possibility is sought, and the possibility is gradually improved along with the development of scientific technology. The application aims at the mechanism of skin aging, adopts technical targeting positioning to develop the active components for tightening and resisting aging, and evaluates the anti-aging effect of the skin tightening composition.

1 mechanism of skin aging

Skin aging is the accumulation of cellular and structural damage that develops as an individual ages, and the skin aging process is divided into intrinsic and extrinsic aspects.

1.1 intrinsic Process

The intrinsic process refers to the phenomenon that as people age, fine wrinkles and loose skin appear on the skin. The endogenous mechanisms of skin aging are very complex, and representative aging theories mainly include a free radical theory, a genetic theory, a mitochondrial DNA damage theory and the like.

1) Theory of free radicals

Free radicals, also known as free radicals, or specifically oxygen radicals, are produced by oxidative metabolism in the body. The theory of free radicals was proposed by Harman in 1956, and its central contents include: free radicals are highly chemically active intermediates that are ubiquitous in living organisms; under normal physiological conditions, the production and elimination of free radicals in the body are in dynamic equilibrium, which, once disrupted, results in the production of excess free radicals; toxic attack of excess free radicals on biological tissues such as chromosomes, mitochondria, cell membranes and connective tissues can cause aging of the body.

The mechanism of aging caused by free radicals can be divided into the following three aspects: firstly, the biomacromolecule is subjected to cross-linking polymerization and lipofuscin accumulation; secondly, organ tissue cells are damaged and reduced; thirdly, the immune function is reduced.

Free radicals promote aging in vivo primarily by: peroxidation of lipids to cause damage to cell membranes; cross-linking or oxidative damage of DNA, RNA to cause cell damage; the polysaccharide polymer is oxidized to cause damage. The theory considers that free radicals with high chemical activity generated in the metabolic process of a body are important initiating factors of aging, and the damage reaction to tissue cells is slow, repeated and gradual, and physiological aging gradually occurs.

2) Theory of genetic origin

The genetics and genetics are the analysis of senescence phenomena at the chromosomal and genetic level. The theory holds that aging is a life phenomenon defined by some genetic program and expressed on time.

With the age, the modified gene is lost, the DNA methylation is reduced, the phosphorylation reaction is reduced, the telomere is shortened, the DNA self-repair capability is reduced, the regulation of protooncogenes, cancer suppressor genes and the like causes chromosome mutation, and normal cells are over-differentiated, so that the aging expression is generated. Hayflik originally developed extracellular in vitro culture to discover cytogenetic laws, thought that developmental processes were temporally sequential, with control mechanisms weakening with age, ultimately leading to senescence.

3) Theory of mitochondrial DNA damage

Miquel and Cowiker presented the mitochondrial theory of senescence in 1980. Damage to mitochondrial DNA is thought to be the molecular basis for cellular senescence and death, and its research has recently become a hotspot for anti-aging mechanisms. From the perspective of cell biology, 90% of energy generated by intracellular oxidative phosphorylation is from mitochondria so as to supply cell life, and when mitochondrial DNA damage occurs, the generated energy is reduced, and energy supply of cells is affected, so that functions of cells, tissues and organs are declined. Thus, researchers believe that mitochondrial degeneration, leakage and rupture are all important causes of aging. Delaying the mitochondrial destruction process may extend the cell life and thus the life of the body.

4) Other theory of learning

Other factors including the theory of variation of enzyme activity, the theory of immunology, the theory of hormones, the theory of telomeres, the absence of essential trace elements, etc. all speculate on the aging mechanism from different angles and levels.

1.2 Exception Process

Extrinsic processes generally refer to the effects of environmental or other forms of exposure on the skin, causing some change. Such as deeper and coarser wrinkles, molting, dry skin, age spots, etc. The exogenous factors mainly comprise ultraviolet irradiation, cigarette smoke and other bad living habits, and the existence of the exogenous factors can accelerate the natural aging process of the skin.

1) Ultraviolet ray

Chronic uv exposure is the most common and most powerful extrinsic factor leading to skin aging. Accumulation of ultraviolet damage the aging of early layers of skin is known as "photoaging". The characteristic changes of photoaging are limited to the exposed part, and are manifested by rough and thick skin exposed part, deepening and thickening of wrinkles, thickening of skin cuticle, gradual change of irregular pigment, dilation or disappearance of capillary vessels, poor epidermis keratosis, and deformation of dermal elastic fiber. It can be seen that there is a difference between natural aging and photoaging. The differences between photoaging and natural aging of skin are shown in the following table.

2) Cigarette smoke

Smoking promotes skin aging, women smoke more easily to be premature or increase the chance of inducing wrinkles, and the effect of promoting skin aging of smoking is equivalent to that of ultraviolet rays, and the two have synergistic effect. Cigarette is the largest source of free radicals, and oxidative damage caused by free radicals makes skin aging and an important promoter of aging-related diseases.

3) Bad life habits

Many undesirable living habits accelerate skin aging, such as: if people stay up for a long time all night, people are worried about being good at feel or are anxious and irritable at ordinary times, people are not suitable for losing weight rapidly or lack of physical exercise, and poor-quality cosmetics, excessive drinking, thick tea, thick coffee and the like are easy to stimulate the skin, reduce the division and proliferation of skin cells, enable the skin to become new metabolic capacity, and enable the skin to be loose to promote aging.

2 anti-aging pathway

Aging is a progressive process of functional and organic decline that all individuals will undergo over time. And the structural changes of the dermis are the main cause of skin aging. Under the combined action of the skin aging endogenous and exogenous factors, the number of the synthetic fiber cells is gradually reduced, the collagen synthesis and elastin synthesis capacity is reduced, the content of aminopolysaccharide in extracellular matrixes is reduced, meanwhile, the proteolytic enzyme is increased, so that the degradation of collagen and extracellular matrix components is increased, the elastic fiber net of the dermal papilla disappears, the elastic fiber is degraded and denatured, trees are reduced, thinned and even fragmented, and the skin is loosened to form wrinkles.

According to the above mechanism, the following approaches are taken to delay skin aging, improve the skin laxity, tighten the skin, improve the skin texture, and reduce the length and depth of wrinkles.

2.1 enhancement of cell proliferation and metabolism

The essence of aging is the decline of tissue cell function. Therefore, promoting the activity of cells, i.e., the proliferative metabolic capacity, and thus delaying skin aging is a key fundamental strategy. The invention finds and selects the bioactive substances from animals, plants and marine organisms, and adopts retinoic acid to promote the epidermal cell conversion and proliferation capacity.

The invention adopts plankton extract (purchased from Baihabo) selected from Saharan algae extract in Saharan desert, the Saharan algae is plankton formed by unicellular algae, and the Saharan algae can resist strong light due to the original morphology and pigment synthesis device, so that the Saharan algae can keep dormancy when the environment is severe and is not suitable for survival, but can be revived when the environment is suitable.

The information on the Baihaobo plankton extract product is as follows:

components	Content%
		Water (W)	55-60
Butanediol	40-45
		Plankton extracts	0.5-2
Arginine	<0.5

The plankton extract adopted by the invention has the effects of skin color uniformity and skin smoothness. Through human body tests, the wrinkle depth can be reduced by 15%, cell detoxification can be performed, mitochondrial activity is improved, oxidized protein regeneration is promoted by stimulating thioredoxin and thioredoxin reductase, biological balance is maintained, and cell activity is increased.

2.2 reconstruction of the extracellular matrix of the skin

The extracellular matrix of skin tissue, mainly connective tissue such as collagen, elastin, mucopolysaccharide and structural glycoproteins, also including lipids and certain natural moisturizing factors, maintains the barrier function and hydration of the skin. The content and quality of extracellular matrix in skin are changed along with various changes of skin aging, and the change is one of important characteristics of skin aging.

Therefore, the constitution of the extracellular matrix, which is reconstructed to make it younger in both quality and quantity, is one of the countermeasures for delaying aging of the skin.

Anti-aging is to increase elasticity from epidermis to dermis, and elasticity is a major factor representing the age of skin, and tight and elastic skin can make people look young for many years. The lack of elasticity is mainly due to the reduced synthesis of immobilized proteins and loss of collagen fibers, resulting in collapse of the interface between dermis and epidermis and the formation of fine lines.

A generic term for a class of organic compounds containing both amino and carboxyl groups. The basic constituent unit of the biological functional macromolecular protein is the basic substance of the protein required by animal nutrition.

Amino acids are actually called chemical units, and they constitute proteins. Proteins provide all living objects with structure. The total of 20 different amino acids constitute proteins, and the amino acids account for 75% of the body's composition. They are essential for almost every bodily function.

The amino acid compound adopted by the invention is added with the same or similar components for forming the horny layer so as to achieve the purposes of keeping the moisture of the skin, providing cell nutrition, proliferating the skin fibroblasts and preventing aging.

Skin effect table of amino acid

Efficacy of	Amino acids
		NMF (Natural moisture keeping factor)	All are
Collagen and elastin Components	Glycine, proline, alanine
		Make up skin cells	Aspartic acid, tyrosine, serine
Cell proliferation	Aspartic acid, serine, proline
		Activating metabolism	Aspartic acid, proline, serine
Removing freckle	Cysteine
		UV protection	Histidine, tryptophan, phenylalanine
Glutathione synthesis	Glutamic acid, cysteine, glycine
		Increasing the immune system	Glutamine
Hair, hair-care product and method for producing the same	Arginine, histidine

Because of their small molecular weight, amino acids readily penetrate into the skin, mostly through the sebaceous glands and follicles. Amino acids are one of the important activities for synthesizing collagen, protein accounts for 16-20% of our body weight, collagen accounts for one third of the protein, and glycine, proline and alanine in the amino acids are synthetic raw materials of the collagen and keep skin elasticity.

Proteins are the basic substances for the growth, development, maintenance and repair of all cellular tissues in the human body and can produce hormones, enzymes, antibodies and neurotransmitters, amino acids being the most basic components constituting these proteins.

Amino acids, which are a source of energy supply to the skin and human body, supply nutrients to each cell to proliferate fibroblasts, promote the synthesis of collagen and elastin in the skin, and provide anti-aging effects.

The composition of the complex amino acids used in the present invention is shown in the following table.

Amino acids	Content%	Amino acids	Content%
				Lysine	4.2	Valine	3.1
ArginineAcid(s)	3.8	Methionine	-
				Aspartic acid	7.9	Isoleucine	2.8
Threonine	3.0	Leucine	7.1
				Serine	13.6	Tyrosine	3.5
Glutamic acid amine	12.6	Phenylalanine	3.3
				Proline	3.0	Histidine	1.5
Glycine	24.5	Cysteine	-
				Alanine	4.4	Total of	100

2.3 anti-expression lines

Wrinkles on the forehead, eyes, periorbital and lips of the human face are evidence of aging, which is also a major problem of skin tightening and anti-aging. These fine lines appear around the age of 30 and are mainly caused by location stress and repetitive facial movements.

The peptides are important physiological regulators of human bodies, can comprehensively regulate the physiological functions of the human bodies, stimulate the regeneration of cells and collagen, and effectively resist aging.

Muscle contraction: occurs at synapses (also known as neuromuscular junctions NMJ), where nerves and muscles participate. The following components are involved in the process: acetylcholine ACH neurotransmitter; ACH-containing neuronal vesicles; ACH receptors in muscle (ACHR).

SNARE complex: vesicle-associated protein membranes (VAMPs), synaptosomal associated proteins (SNAP-25), and syntaxin. This complex is responsible for the release of ACH into the synapse.

The release of ACH is critical for facial muscle contraction. After stimulation of ACH-containing neuronal vesicles, the SNARE complex assembles and releases neurotransmitters into the synapse. The ACHR then binds to ACH, causing muscle contraction.

Botulinum neurotoxins, a family of natural neurotoxins, are widely used in cosmetics to reduce expression wrinkles, and cause muscle paralysis (toxicity) by selectively blocking the release of ACH at the neuromuscular junction by cleaving SNAP-25 protein.

The invention adopts acetylated hexapeptide (wrinkle removing peptide) and a botulinum toxin component, which has the function of botulinum toxin and blocks the muscle nerve transmission speed, thereby inhibiting the generation of expression lines and fine wrinkles, but having no toxicity of the botulinum toxin. It competes with SNAP-25 for position in the SNARE complex, thereby modulating the course of the complex, interfering with complex formation and stability, but is very safe.

2.4 anti-free radicals

The damage of active oxygen free radicals generated by counter ions and low-energy radiation to skin lipid, protein and biomembrane. At present, the effects of resisting and eliminating free radicals and preventing the damage of the free radicals are achieved by antioxidants.

The oxidation is the biggest threat to the skin, the skin can be flooded by free radicals due to unhealthy diet, sunshine, pressure, environmental pollution and the like, and the oxidation phenomena of complexion ammonia nitrogen, water shortage and the like are generated. For cosmetics, antioxidants fall into two broad categories-physical antioxidants and active antioxidants. The former is mainly used to stabilize the product matrix, the latter is responsible for the functional efficacy in cosmetics.

The present invention employs vitamin E.

Vitamin E refers to α -a bioactive class of tocopherol, which is produced in the sebaceous glands of humans and is the most abundant antioxidant found in the skin, when the vitamin E content of the skin is reduced, the sebaceous gland endocrine α -tocopherol and β -tocopherol are transmitted to the surface of the skin through sebum, acting as a first line of defense against external environmental stresses.

When the sebaceous gland secretion is lower than the skin consumption, a series of oxidative damages of the skin occurs. The role played by sebaceous glands and sebum in the production and transport of vitamin E may explain the phenomenon of oily skin being slower than dry skin.

Vitamin E can permeate skin and accumulate continuously, so that the skin becomes smooth and tender, the effects of delaying senility, inhibiting erythema solare, reducing wrinkles, moistening the skin, diminishing inflammation and the like are achieved, and the vitamin E is a good active substance for delaying the senility.

2.5 resistance to crosslinking

The crosslinking of collagen and elastic fibers in the dermis is one of the important causes of aging, such as skin wrinkles and decreased elasticity. Many metal ions are a kind of crosslinking agent, and therefore, a crosslinking reaction is inhibited by complexing the metal ions using citrate, EDTA or the like. Anti-crosslinking agents may be used, including anti-glycosylation. Anti-glycosylation is considered to be de-cross-linking from the inside of the fibroblasts.

Glycated collagen loses the flexibility and elasticity of the skin and wrinkles.

This equation clearly shows that the key to wrinkles is collagen deglycation, which occurs inside or outside the fibroblasts, so that, as long as the glycation occurring in the fibroblasts can be reduced, the skin can maintain elasticity and wrinkles can be reduced.

The present invention uses decarboxylated carnosine (from Baihabo) purchased as an aqueous polyol solution containing 10% decarboxylated carnosine, and is a very stable transparent liquid, colorless to slightly yellow, with a slight taste.

The decarboxylated carnosine (Alistin) adopted by the invention can mainly compete with reactants, and a reaction activator is removed: free radicals, thereby preventing the protein from glycated crosslinking.

2.6 resistance to degradation

The present invention is directed to combating the degradation of the extracellular matrix of skin by various hydrolytic enzymes. For example, against the degradation of collagen, elastin and hyaluronic acid by collagenase, elastase and hyaluronidase, respectively, with consequent deterioration of the skin structure and acceleration of ageing, in particular by elastase, which causes the skin to lose its elasticity, to relax and to wrinkle.

The hydroxyproline biological carrier is prepared by adding SEPILIFT DPHP (dipalmitoyl hydroxyproline), which is a main component of collagen, hydroxyproline responsible for the structure and compactness of dermis and palmitic acid derived from plants through acylation reaction.

Skin collagen fibers are less contracted, free radical attacks, and the dermal matrix arrangement is disrupted, which can lead to skin laxity, loss of elasticity, and the formation of wrinkles and crow's feet. According to the invention, dipalmitoyl hydroxyproline is adopted to carry out all-round defense to bring younger skin, and the triple tightening effect is mainly embodied: 1) stimulating collagen fiber contraction; 2) eliminating free radicals; 3) protecting dermal extracellular matrix, resisting elastase, increasing procollagen synthesis, inhibiting metalloprotease-2 synthesis, and reducing metalloprotease-2 production. The integrity of the dermis and epidermis connecting layer is protected, and the effect is shown as follows according to the in vitro test of a human body, and the effect of removing expression lines is certain.

2.7 anti-allergic inflammation

Environmental factors are increasingly attracting attention to accelerate the aging process of the skin through damage to the skin. Among the environmental factors, in addition to ultraviolet-induced photoaging, air pollution caused by carbon dioxide, smoke and exhaust gas from automobile exhaust is the most important. Skin damage caused by environmental pollution is common, harmful dust in polluted air is deposited on the skin and shows allergic inflammatory reaction, but the skin burn and erythema caused by solar radiation are not easy to detect, so that the anti-allergic inflammation is also one of important strategies for delaying skin aging.

The invention adopts lactobacillus/soya-bean milk fermentation product filtrate (purchased from Xianting), and the lactobacillus is a beneficial microbial flora in a bacterium group capable of secreting lactic acid and has high compatibility with skin and human body. Lactobacilli can produce: organic acids such as lactic acid, proteases which are beneficial to the skin by lactic acid bacillase, glucosidase, etc. Phytochemicals fermented with lactobacilli are more potent and they are usually degraded during traditional extraction, produce more active ingredients, improve bioavailability, and are mild and safe.

The information of the filtrate product of the lactobacillus distachys/soybean milk fermentation product is as follows:

components	Content%
		Water (W)	81
Lactobacillus/soymilk fermentation product	10
		Butanediol	7
1, 2 pentanediol	2

2.8 cellular repair

The collagen in the dermis is reduced, the elasticity of the skin is reduced, and wrinkles are generated. Thus promoting collagen growth also relieves skin aging. The invention adopts vitamin A (retinol) palmitate and hydrolyzed collagen to carry out anti-aging repair.

Retinol palmitate

Retinol, the most widely present form of vitamin a in the skin, is absorbed through the skin and helps to reduce fine lines and wrinkles, maintain the skin soft and plump, improve the function of the skin as a water barrier, and also increase skin elasticity and improve skin tone and texture.

Hydrolyzed collagen

During the aging process, the structure of collagen in the skin is gradually changed from spiral soluble collagen into mutually interlaced insoluble collagen, and the synthetic capacity of fibroblasts is reduced, so that the skin is dried, loses flexibility and has reduced elasticity.

The hydrolyzed collagen can be absorbed by skin and filled between skin substrates, so as to make skin plump and wrinkle stretched, and simultaneously, the collagen can also improve skin density, increase skin elasticity, stimulate skin microcirculation and promote skin metabolism, so as to make skin smooth and bright and reduce wrinkles.

The skin tightening composition (uniboost) adopts anti-aging vitamins, amino acids, derivatives and peptides, improves an extracellular matrix structure from the endogenous source, adopts dipalmitoyl hydroxyproline, palmitoyl glycine, decarboxylated carnosine, acetylated hexapeptide, sodium hyaluronate, retinol, hydrolyzed collagen, plankton extract, lactobacillus/soybean milk fermentation product filtrate and dipotassium glycyrrhizinate as main components, and improves the tightening and elasticity of the skin and restores the young skin by promoting the synthesis of collagen and elastin and inhibiting the activity of metalloproteinase aiming at wrinkles and comprehensively restoring fine lines generated by skin relaxation.

Drawings

FIG. 1 is a graph of the amount of change in skin elasticity for example samples 1-3 and for control samples 1-5;

FIG. 2 is a graph showing the increase in the degree of fatigue resistance of skin of example 1-3 and comparative example 1-5.

Detailed Description

In order that the invention may be more fully understood, reference will now be made to the following description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

The skin tightening composition comprises the following components:

the application develops compact components on the basis of a moisturizing basic formula, and the proportion of raw materials of an example sample and a reference sample is as follows:

the preparation process comprises the following steps:

taking example 1 as an example, 1 part by mass of PHL as an antibacterial agent, 0.1 part by mass of carbomer 20 and 0.1 part by mass of arginine as a thickener were added, and deionized water was added to 100 parts by mass. The preparation process of the essence comprises the following steps:

(1) weighing the process water according to the process proportion;

(2) respectively weighing glycerol, propylene glycol, butanediol, betaine, allantoin, trehalose, oligomeric hyaluronic acid, hydrolyzed collagen, carbomer 20, arginine and the extract of the Arabinorhizobium, heating to 75-80 ℃, stirring for 10min at 200r/min under 150-;

(3) respectively weighing dipalmitoyl strong proline and compound amino acid, heating to 90-93 ℃, stirring for 50min at 250r/min of 200-;

(4) reducing the stirring speed to 150-;

(5) reducing the temperature by 45-50 ℃, weighing the preservative PHL at 80-100r/min, and stirring for 5 min.

Efficacy evaluation

The test method was carried out by connecting an elastic probe PVM600 manufactured by Courage + Khazaka, Germany (CK, Germany) to an MPA master according to a standard method and using the probe in combination with CutomerQ analysis software.

(1) Working principle of skin elasticity test probe

The test principle is based on the principle of suction and stretching, where a negative pressure is generated on the skin surface to be tested to suck the skin into a specific test probe, and the depth of the skin sucked into the test probe is measured by a non-contact optical test system. The test probe includes a light emitter and a light receiver, the ratio of light (the ratio of emitted light to received light) is proportional to the depth of skin being absorbed, thus obtaining a curve of the length of skin stretched versus time, which is then analyzed by the MPA software to determine the elastic properties of the skin.

Technical parameter indexes of the skin elasticity test probe are as follows:

host physical size: 27X25.5X7cm, probe: phi 2X10cm

Probe test hole diameter: phi 2mm

Pressure: (20-500) mbar

Test time: (0.1-60) seconds

Precision: penetration depth of about 3% above 100um, and wireless property below 100um

Power supply: 100 to 240V/50-60Hz/0.3A

Interface: USB (universal serial bus)

Test negative pressure mode: continuous negative pressure mode

Curve display mode: stretch length-time table

Skin elasticity test mode-constant negative pressure maintenance

Uf＝Ue+Uv

Wherein: uf-maximum skin stretch with negative pressure.

Ue — the amount of skin stretch at 0.1 second after a constant negative pressure is applied to the skin, the amount of stretch in the elastic portion is located.

Uv-Uf-Ue is the amount of stretching of the viscoelastic part, or plastic part, of the skin.

The value of Ue is higher for skin that is more elastic than for younger skin, whereas the value of Ue is lower for skin that is less elastic than for older skin, and the value of Uv is higher for viscoelastic.

Ur＝Uf-U1.1 Ua＝Uf-U2.0

Wherein: ur-the value of elastic part Ur, i.e. the value of elastic part and the value of viscoelastic part, also called plastic part, after negative pressure is removed for 0.1 second.

Ua-the recovery value of the skin from the removal of negative pressure to the next successive test of the skin surface plus negative pressure.

The younger the skin, the more elastic the skin, the higher the elastic fraction value Ur; the older the skin, the less elastic the skin, the lower the Ur value.

Skin elasticity test parameters:

test mode: 1

Negative pressure: 450mbar

·On-time：2.0s

·Off-time：2.0s

Cycle number: 10

Preparation time: 5.0s

Skin elasticity test method:

opening the Curometer Q software, clicking the tool bar Measurement or directly clicking the upper right Measurement button, taking down the probe cover after the pump is started, clicking OK, vertically and lightly contacting the probe with the skin area to be tested within 30 seconds (the skin surface should be cleaned, if a product is smeared, the test is carried out after the product is absorbed, so as not to pollute the probe hole), starting the test until the test cycle time is over, moving the probe away, and covering the probe cover. The 3-point test was performed in the area 2.5X 2.5cm from the arm's upper arm wrist, and the average value of R2 was calculated, R2 ═ Ua/Uf. Meanwhile, the anti-fatigue degree value F4 of the skin can be calculated according to the measured data. After the area was covered with a mask cloth, 0.45ml of the sample to be tested was injected into the mask cloth, the mask cloth was removed after 20 minutes of application, the mask liquid was washed off after 10 minutes, and the area was tested for skin elasticity value R2 (shown in FIG. 1 and Table 1) and skin fatigue resistance value F4 (shown in FIG. 2 and Table 2) after the skin was dried.

TABLE 1

TABLE 2

In conclusion, through the test results of the elasticity of the skin and the degree of fatigue resistance of the skin over time, it can be found that the treated area of the example sample has a certain effect on the elasticity and the degree of fatigue resistance, particularly has a good effect when measured for a long time continuously, compared with the blank sample (water treatment), and the skin tightening composition of the present invention has a significant effect on the elasticity and the degree of fatigue resistance of the skin.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The skin tightening composition is characterized by comprising the following raw material components in parts by mass:

hyaluronic acid 0.01-0.2

Hydrolyzed collagen 0.01-0.3

Dipalmitoyl hydroxyproline 0.01-1

0.01-1 parts of compound amino acid

Decarboxylated carnosine 0.01-0.5

Acetylated hexapeptide 0.01-0.5

Plankton extract 0.01-0.03

Lactobacillus/soybean milk fermentation product filtrate 0.05-0.5

Dipotassium glycyrrhizinate 0.1-0.5

Retinol palmitate 0.05-0.5

VE 0.1 - 1

Hydrolyzed sesame protein 0.03-2.

2. The skin tightening composition is characterized by comprising the following raw material components in parts by mass:

hyaluronic acid 0.01

Hydrolyzed collagen 0.5

Dipalmitoyl hydroxyproline 1

Complex amino acid 1

Decarboxyme carnosine 0.1

Acetylated hexapeptide 0.05

Plankton extract 0.1

Lactobacillus/Soy milk fermentation product filtrate 0.5

Dipotassium glycyrrhizinate 0.2

Retinol palmitate 0.1

VE 0.5

Hydrolyzed sesame protein 0.5.

3. The skin tightening composition according to any one of claims 1-2, wherein the complex amino acid comprises the following components in percentage by mass:

4. use of the skin tightening composition of any of claims 1-3 in the preparation of a cosmetic product.

5. The use according to claim 4, characterized in that the cosmetic product comprises a face wash, a lotion, a cream, a serum, a cream, a mask or a makeup.

6. A cosmetic product characterized by comprising the skin tightening composition according to any one of claims 1 to 3.