CN107307192A - A kind of feed cultivated for ganoderma lucidum soft-shelled turtle and its cultural method - Google Patents
A kind of feed cultivated for ganoderma lucidum soft-shelled turtle and its cultural method Download PDFInfo
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- CN107307192A CN107307192A CN201710662267.0A CN201710662267A CN107307192A CN 107307192 A CN107307192 A CN 107307192A CN 201710662267 A CN201710662267 A CN 201710662267A CN 107307192 A CN107307192 A CN 107307192A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to aquaculture field, and in particular to a kind of feed cultivated for ganoderma lucidum soft-shelled turtle and its cultural method, the feed include the preparing raw material of following parts by weight：0.5~2 part of 10~25 parts of lucidum spore powder, 10~20 parts of sweet potato powder, 10~20 parts of taro meal, 10~20 parts of dehydrated potato powder, 15~22 parts of α starch, 6~12 parts of white fish meal, 6~12 parts of red fish meal and mould inhibitor.Scientific and reasonable, the comprehensive nutrition of feed formula collocation of the present invention, feed conversion rate is high, and the culture-cycle of soft-shelled turtle is short (2~3 years), and the soft-shelled turtle through the forage feed, meat is soft, and health value is high.
Description
Technical field
The invention belongs to aquaculture field, and in particular to a kind of feed cultivated for ganoderma lucidum soft-shelled turtle and its cultural method.
Background technology
Soft-shelled turtle contains abundant protein, fat, vitamin and mineral, and nutritious, delicious flavour is that enriching yin is mended
The common drug among the people of sun, broken knot softening hard masses.The meat flavour of soft-shelled turtle is delicious, is a kind of senior aquatic products, traditional Chinese medicine thinks that it has promoting blood circulationization
Become silted up, effect of nourishing and fit keeping function, generally using it as after being ill or the weakly nourishing health-care food of Post operation.But excessively exploitation
And the continuous destruction of Natural Survival environment so that wild Shelled Turtle Trionyx Sinensis resource is reduced, and manually foster soft-shelled turtle industry is developed rapidly.But it is high
The intensive cultivation of density so that the quality decline of commodity soft-shelled turtle, mouthfeel reduction.With the further depth of China's agricultural structure
Enter adjustment and people's living standard, the further lifting of consumption idea, safety cultured product of fine quality turns into main development
Direction.
As the fast-developing and intensive large-scale degree of breeding soft-shell turtle industry is improved, the dependence to mixed feed also increases therewith
By force.The soft-shelled turtle compound feed generally existing of current China the too high phenomenon of protein content, such as Chinese patent application
CN102511694B discloses a kind of ecological soft-shelled turtle compound feed, and the component of feed and the parts by weight of each component are fish meal 50-56
Part, 20-24 parts of alphalise starch, 3-5 parts of fermented bean dregs, 1-3 parts of expanded soybean, 1-2 parts of liquid fish oil, 1-3 parts of beer yeast powder, egg
1-2 parts of propylhomoserin, 1-2 parts of lysine, 1-2 parts of skimmed milk power, 1-2 parts of yolk powder, 0.5 part of composite mineral matter, B B-complex 0.4
Part, 1.5 parts of calcium dihydrogen phosphate, 0.1 part of phytase, 0.2 part of Chinese herb feed additive, 1 part of seawood meal, 1 part of lecithin, beta glucan
0.1 part, 0.1 part of Proheparin.Chinese patent application CN102217749A discloses a kind of biological healthcare-type compound feed for soft-shelled turtles, its
Raw material components are：By mass percentage, white fish meal 30-40%, red fish meal 10-20%；Swelling soya dreg 4-10%；Dusty yeast 6-
12%；A- starch 19-22%；Fermented bean dregs 5-8%；Gluten 2-5%；Calcium dihydrogen phosphate 2-5%；Enzyme preparation 0.05-
0.3%；Microorganism formulation 0.1-0.3%；Compound vitamin 0.1-0.5%；Composite polymineral 1-3%；50% Choline Chloride 0.2-
0.8%；Liver and gallbladder protection additive 0.1-0.5%, intestinal mucosa repair additive 0.3-0.6%.But albumen in above-mentioned feed
Matter too high levels, feed is easily mouldy, and storage life shortens, while having aggravated the burden of liver of soft-shelled turtle again, causes a series of metabolism
Property disease, cause that soft-shelled turtle is slow-growing, immunity degradation after feeding for a long time, the general level of the health of soft-shelled turtle in reduction breeding process.
Therefore, it is necessary to a kind of steady quality be provided, while having growth promotion concurrently and improving the feed of immunity.
The content of the invention
The present invention is intended to provide a kind of feed cultivated for ganoderma lucidum soft-shelled turtle and its cultural method, the feed with the addition of ganoderma lucidum spore
Sub- powder, the soft-shelled turtle obtained with the forage feed is in good taste, has effects that tonifying Qi of invigorating blood circulation.
In order to achieve the above object, the present invention uses following technical scheme：A kind of feed cultivated for ganoderma lucidum soft-shelled turtle, including
The preparing raw material of following parts by weight：10~25 parts of lucidum spore powder, 10~20 parts of sweet potato powder, 10~20 parts of taro meal, Ma Ling
0.5~2 part of 10~20 parts of potato powder, 15~22 parts of alphalise starch, 6~12 parts of white fish meal, 6~12 parts of red fish meal and mould inhibitor.
Further, the feed includes the preparing raw material of following parts by weight：20 parts of lucidum spore powder, sweet potato powder 15
Part, 15 parts of taro meal, 12 parts of dehydrated potato powder, 18 parts of alphalise starch, 9 parts of white fish meal, 9 parts of red fish meal and 1.5 parts of mould inhibitor.
Further, the feed includes the preparing raw material of following parts by weight：10 parts of lucidum spore powder, sweet potato powder 10
Part, 10 parts of taro meal, 10 parts of dehydrated potato powder, 15 parts of alphalise starch, 6 parts of white fish meal, 6 parts of red fish meal and 0.5 part of mould inhibitor.
Further, the feed includes the preparing raw material of following parts by weight：25 parts of lucidum spore powder, sweet potato powder 20
Part, 20 parts of taro meal, 20 parts of dehydrated potato powder, 22 parts of alphalise starch, 12 parts of white fish meal, 12 parts of red fish meal and 2 parts of mould inhibitor.
Further, the mould inhibitor is acid-producing bacteria fermentation dry powder.
Further, the acid-producing bacteria fermentation dry powder is made by following steps：
The ring of picking acid-producing bacteria one is inoculated in the 250ml triangular flasks for filling 20~30ml fermentation mediums, 32 DEG C of shaking table shakes
Swing 12~18h of culture, 100~150r/min of rotating speed；Zymotic fluid is taken, 3500r/min centrifuges 10~15min, takes supernatant, freezes
It is dry, produce；Wherein, the fermentation medium pH is 6.0, containing glucose 15~20%, yeast extract 0.1~0.5% and water 80~
85%.
Further, the one kind of the acid-producing bacteria in Propionibacterium, lactic acid bacteria and acetobacter.
Further, the acid-producing bacteria is selected from Propionibacterium.
Further, the feed also includes 0.05~1 part of Human plactnta mescenchymal stem cell extract freeze-drying powder.
Further, the Human plactnta mescenchymal stem cell extract freeze-drying powder is made by following steps：
S1, the DMEM medium culture Human plactnta mescenchymal stem cells using pH6.5~7.0, containing serum to 70~80%
During fusion, its former serum-containing medium is abandoned, cell is washed with D-Hank ' s balanced salt solutions；
S2, removal balanced salt solution, use the DMEM culture mediums containing serum to continue to cultivate Human plactnta mescenchymal stem cell, training
Support to cell culture medium discoloration, collect supernatant；
S3, filtering supernatant, concentration are degerming, add freeze-dried excipient and water, freeze, produce.
Correspondingly, present invention also offers a kind of method for preparing above-mentioned feed, comprise the following steps：
Lucidum spore powder is taken, is crushed, sweet potato powder, taro meal, dehydrated potato powder, alphalise starch, white fish meal, red fish meal is added and prevents
Mould dose, it is well mixed, sieves, packing is produced.
Another object of the present invention is to provide a kind of ganoderma lucidum soft-shelled turtle cultural method, is included in and is monthly fed to soft-shelled turtle within first 10~15 days
The feed that above-mentioned feed or the above method are prepared, monthly after 15~20 days give soft-shelled turtle feeding soft-shelled turtle powder, day inventory account for soft-shelled turtle body
The 2~4% of weight, fed through 24~36 months, go out the pool.
Protein content compares Chinese patent application CN102511694B and Chinese patent application in feed of the present invention
CN102217749A is relatively low, but the storage life of feed still also has the space lifted.Inventor chances on acid-producing bacteria
Zymotic fluid there is certain antibacterial, mildew-proof function, investigated by many experiments and data, optimal production acid preferably gone out finally
Bacterium, more preferably Propionibacterium, lactic acid bacteria and acetobacter, Propionibacterium.Experiment is proved, be with the addition of fermentation using propionibacterium and is done
The feed of powder, after storage the 55th day, total number of molds is 6521 × 104, and the feed of fermentation using propionibacterium dry powder is not contained,
Preserve after identical number of days, total number of molds reaches 18952 × 10 in feed4.The generation mechanism of above-mentioned special-effect, still fails to understand
Really, it has been recognised by the inventors that being probably that acid-producing bacteria zymotic fluid contains organic acid, and organic acid has corrosiveness, adds in zymotic fluid
Some other compositions interaction so that it has excellent mould proof effect.It should be noted that and organic acid
Compare, the anti-mold effect that it is reached is more excellent, this is probably relevant with some other compositions present in zymotic fluid.
On the other hand, after containing because soft-shelled turtle is edible compared with the feed of high protein content, excretory material is nitrogenous, phosphorus, highdensity
Cultivating causes water body nitrogen, phosphorus severe overweight, and the concentration of narmful substance such as ammonia is excessive, and water quality deterioration is seriously polluted, and soft-shelled turtle gives birth to for a long time
Living constitution degradation, immunologic function is gone wrong, and disease often occurs, and medicine is frequently used in this environment, is formed
The vicious circle of breeding soft-shell turtle process.In such a circulation, feed is main influencing factor.Therefore, if
A kind of immunopotentiator is added in feed, that is, is possible to solve the above problems.It has passed through prolonged research, inventor
Develop a kind of immunopotentiator, Human plactnta mescenchymal stem cell extract, it can significantly improve GSH-PX in soft-shelled turtle serum
(glutathione peroxidase), T-SOD (total hepatocuprein) and LSZ (lysozyme) vigor, while significantly reducing first
AST (glutamic-oxalacetic transaminease) and ALP (alkaline phosphatase) vigor in fish serum.Meanwhile, the presence of the immunopotentiator can also drop
Low soft-shelled turtle myofibril diameter, improves the content of albumen and fat in soft-shelled turtle muscle, lifts soft-shelled turtle meat guality.
In addition, the present invention is first toward adding lucidum spore powder in feed, the soft-shelled turtle obtained through the forage feed, health value
Height, it is nutritious, it is in good taste.The soft-shelled turtle general culture-cycle is 2~4 years, and the cultivation week of soft-shelled turtle can be made by feeding feed of the present invention
Phase shorten to 2~3 years.
The present invention has advantages below：
1) scientific and reasonable, the comprehensive nutrition of feed formula collocation of the present invention, feed conversion rate is high, and the culture-cycle of soft-shelled turtle is short,
Soft-shelled turtle through the forage feed, meat is soft, and health value is high.
2) presence of immunopotentiator of the present invention can improve the immunologic function of soft-shelled turtle, while reducing muscle fibril in soft-shelled turtle
Diameter, improve soft-shelled turtle muscle in albumen and fat content.
3) feeding quality of the present invention is stable, and anti-mold effect is good, and long shelf-life is significantly better than normal diet.
Embodiment
The embodiment of form, further specifically to the above work of the present invention by the following examples
It is bright.But the scope that this should not be interpreted as to above-mentioned theme of the invention is only limitted to following examples.
Lucidum spore powder of the present invention is purchased from Guangzhou Yu Zhi gardens bio tech ltd；Sweet potato powder is purchased from the golden grain in Guangzhou
Product Co., Ltd；Taro meal is purchased from Jiangsu Zhen Ya Food Co., Ltd；Dehydrated potato powder is purchased from Xinghua City Lv Bao Food Co., Ltd；
White fish meal, red fish meal are purchased from Guangdong Zhong Si Industry Co., Ltd；Soft-shelled turtle powder grows bio tech ltd purchased from Xi'an ten thousand.
The preparation of embodiment 1, fermentation using propionibacterium dry powder
The ring of picking Propionibacterium one is inoculated in the 250ml triangular flasks for filling 30ml fermentation mediums, 32 DEG C of shaking table concussions
Cultivate 12h, rotating speed 150r/min；Zymotic fluid is taken, 3500r/min centrifugation 10min take supernatant, freeze, produce；Wherein, it is described
Fermentation medium pH is 6.0, containing glucose 15%, yeast extract 0.5% and water 84.5%.
The preparation of embodiment 2, lactobacillus-fermented dry powder
The ring of picking lactic acid bacteria one is inoculated in the 250ml triangular flasks for filling 30ml fermentation mediums, 32 DEG C of shaking table concussion trainings
Support 12h, rotating speed 150r/min；Zymotic fluid is taken, 3500r/min centrifugation 10min take supernatant, freeze, produce；Wherein, the hair
Ferment medium pH is 6.0, containing glucose 15%, yeast extract 0.5% and water 84.5%.
The preparation of embodiment 3, Human plactnta mescenchymal stem cell extract freeze-drying powder
(1) placenta sample disposal：Mature, Cesarean esction is obtained from Guangzhou, Guangdong Grade A hospital gynaecology and obstetrics operation platform, be good for
Health placenta materna, through inspection and quarantine it is qualified after (confirm without communicable diseases such as HIV, HBV), aseptically divest parent and slough off
Film, clip fritter embryonic decidua side placenta tissue, is put into the D-Hank ' s liquid containing antibiotic, washes most blood, rejects blood vessel, and
It is cut into 1.0mm3Fritter, is disappeared with the digestive juice of the trypsase containing mass percent 0.25% and mass percent 0.02%EDTA
Change, obtain cell suspending liquid；
(2) discontinuous Percoll Graded Densities separation：Percoll points into 7 density are successively spread in 50mL centrifuge tubes
Chaotropic, each density 5mL, then 5mL cell suspensions are slowly added into, 1200g centrifuges 20min to use level centrifuge at room temperature, small
Liquid more than heart reject centrifuge tube 20mL scales, collects the cellular layer and 12.5~7.5mL scales of 12.5~20.0mL scales
Liquid；The visible obvious white cloud cellular layer at centrifuge tube 15~20mL scales, corresponding Percoll is relative herein
Density is 1.046~1.059, is the region that trophocyte is present；Visible unconspicuous cellular layer near centrifuge tube 10mL scales,
Density is 1.072 herein, is the region that lymphocyte and placenta mesenchyma stem cell are present；It is respectively put into different centrifuge tubes,
Diluted with D-Hank ' s liquid after 5 times, 1000g centrifuges 15min, centrifuge tube bottom visible white cell mass at room temperature；
(3) purify：With containing DMEM/F12, acetonate, 10 mg/litres of the volume fraction for 10% hyclone (FBS)
The bFGF of sodium selenite insulin, 2 mg/litre monoethanolamines and 20 nanograms/mL is configured to suspension, after density gradient centrifugation
Trophocyte be resuspended, with differential attachment method remove fibroblast after count, the trophocyte amount of acquisition is up to 6.15
×108It is individual；
(4) passage and identification：Above-mentioned trophocyte is inoculated in and adds the DMEM/F12 trainings containing volume fraction 15%FBS
Nutrient solution is positioned over 37 DEG C, the CO of volume fraction 5%2Cultivate in saturated humidity incubator, fresh medium is added per 3d, by 1 ×
104cell/cm2Secondary Culture, nutrient solution is changed to volume fraction 10%FBS DMEM/F12 culture mediums, and cell algebraically is designated as P1
Generation；After cell reaches 70~90% fusions, digestion, by 1 × 104cell/cm2Secondary Culture, cell algebraically is designated as P2 generations, receives
Collect P2 for cell；By flow cytometry cell surface antigen, testing result shows obtained cell and filled between Human plactnta
Matter cells and characteristic of stem is consistent, illustrates to be separately cultured cell behaviour placenta mesenchyma stem cell；
(5) when using pH7.0, the DMEM medium culture Human plactnta mescenchymal stem cells containing serum to 80% fusion, abandon
Its former serum-containing medium, cell is washed with D-Hank ' s balanced salt solutions；Balanced salt solution is removed, the DMEM containing serum is used
Culture medium continues to cultivate Human plactnta mescenchymal stem cell, and culture collects supernatant to cell culture medium discoloration；Filtering supernatant,
Concentration, it is degerming, sweet dew alcohol and water is added, freezes, produces.
Feed formula described in the embodiment of the present invention 4~6 and its consumption (parts by weight)
Preparation method：
Lucidum spore powder is taken, is crushed, sweet potato powder is added, taro meal, dehydrated potato powder, alphalise starch, white fish meal, red fish meal, anti-
Mould dose and immunopotentiator, are well mixed, and cross 100 mesh sieves, and packing is produced.
Embodiment 7, a kind of cultural method
Monthly feed any feed of embodiment 4~6 to soft-shelled turtle within first 15 days, monthly give soft-shelled turtle to feed soft-shelled turtle powder within latter 15 days, feed intake day
Amount accounts for the 3% of soft-shelled turtle body weight, was fed through 36 months, goes out the pool.
Comparative example 1, a kind of feed cultivated for ganoderma lucidum soft-shelled turtle
The difference of comparative example 1 and embodiment 5 is：Fermentation using propionibacterium dry powder is replaced with propionic acid, remaining parameter and operation
Such as embodiment 5.
The influence of experiment one, feed of the present invention to soft-shelled turtle growth, meat and immunity
Three greenhouses are chosen, A groups, B groups and C groups are respectively set as, A groups feed commercially available routine by the methods described of embodiment 7
Feed；B groups cultural method as described in embodiment 7 feeds the feed of the embodiment of the present invention 4, and C groups cultural method as described in embodiment 7 is thrown
Feed the feed of the embodiment of the present invention 5.Every greenhouse totally 14 culturing pools, specification 10m × 10m, water body depth 60cm, each culturing pool is thrown
Soft-shelled turtle soft-shelled turtle is put, only, cultivation density is 30/m to average weight (226.96 ± 32.21g)2, young soft-shelled turtle raises and train one in greenhouse cultivation pool
It is formal after week to start experiment, cultivate 20 weeks.
In process of the test, feed daily 3 times (7:00-8:00,12:00-14:00,20:00-21:00), daily ration, feeding quantity 1.5-
2kg/ ponds/time, feed is squeezed into graininess, fed under water, free choice feeding, and patrol pool inspection food throwing feeding half an hour after
Feeding amount is thrown in situation, in time adjustment, and residual feed is cleaned in time.Controlled during cultivation in water temperature (30 ± 2) DEG C, pH7.0-7.8, water
Nitrite nitrogen content is less than 0.2mg/L, and ammonia-nitrogen content is less than 5mg/L, and dissolved oxygen is in 4-6mg/L.
Result of the test is analyzed with SPSS softwares, result of the test such as table 1 below~3.
Influence of the table 1 to soft-shelled turtle growth performance
Note：Compared with A groups,**P ＜ 0.01,*P ＜ 0.05.
From upper table 1, the feed of immunopotentiator (Human plactnta mescenchymal stem cell extract freeze-drying powder), energy with the addition of
Enough pole remarkably promotes the growth of soft-shelled turtle, and pole significantly improves soft-shelled turtle rate of body weight gain and survival rate, and difference has conspicuousness.
Influence of the table 2 to soft-shelled turtle meat
Note：Compared with A groups,**P ＜ 0.01,*P ＜ 0.05.
From upper table 2, the feed of immunopotentiator (Human plactnta mescenchymal stem cell extract freeze-drying powder), energy with the addition of
The muscle protein of soft-shelled turtle and the content of fat are enough significantly improved, the diameter of soft-shelled turtle muscle fibril is significantly reduced, difference has notable
Property.This explanation, feed of the present invention can lift the meat guality of soft-shelled turtle.
Influence of the table 3 to immune indexes in soft-shelled turtle serum
Note：Compared with A groups,**P ＜ 0.01,*P ＜ 0.05.
As shown in Table 3, the feed of immunopotentiator (Human plactnta mescenchymal stem cell extract freeze-drying powder) is with the addition of, can
Significantly improve GSH-PX (glutathione peroxidase) in soft-shelled turtle serum, T-SOD (total hepatocuprein) and LSZ (bacteriolyzes
Enzyme) vigor, while significantly reducing the vigor of AST (glutamic-oxalacetic transaminease) and ALP (alkaline phosphatase) in soft-shelled turtle serum, this says
It is bright, in the presence of Human plactnta mescenchymal stem cell extract freeze-drying powder, the immunity of soft-shelled turtle can be significantly improved.
Experiment two, anti-mold effect experiment
The embodiment of the present invention 5, embodiment 6 and the feed 200g of comparative example 1 are taken, three groups of feeds are investigated in different storage number of days
When feed mold situation, count fungi count, result of the test such as table 4.
The embodiment of the present invention 5~6 of table 4 and the feed of comparative example 1 in different storage number of days fungi count statistics (unit/
104)
Note：The method of inspection that fungi count method of counting presses mould in national standard GB/T13092-1991 feeds is carried out.
As shown in Table 4, each group fungi count is ordered as from more to less：Between the ＞ embodiments 5 of 1 ＞ embodiments of comparative example 6, each group
Difference was obvious, especially comparative example 1, in storage the 35th day, and feed mould number has reached that the mould of the storage 55 days of embodiment 5 is total
Several 193.28%, this explanation, compared with existing propionic acid mould inhibitor, mould inhibitor of the present invention has more excellent anti-mold effect.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of feed cultivated for ganoderma lucidum soft-shelled turtle, it is characterised in that include the preparing raw material of following parts by weight：Reishi sporule
10~25 parts of powder, 10~20 parts of sweet potato powder, 10~20 parts of taro meal, 10~20 parts of dehydrated potato powder, 15~22 parts of alphalise starch, whitefish
0.5~2 part of 6~12 parts of powder, 6~12 parts of red fish meal and mould inhibitor.
2. feed as claimed in claim 1, it is characterised in that include the preparing raw material of following parts by weight：Lucidum spore powder
20 parts, 15 parts of sweet potato powder, 15 parts of taro meal, 12 parts of dehydrated potato powder, 18 parts of alphalise starch, 9 parts of white fish meal, 9 parts of red fish meal and mould proof
1.5 parts of agent.
3. feed as claimed in claim 1 or 2, it is characterised in that the mould inhibitor is acid-producing bacteria fermentation dry powder.
4. feed as claimed in claim 3, it is characterised in that the acid-producing bacteria fermentation dry powder is made by following steps：
The ring of picking acid-producing bacteria one is inoculated in the 250ml triangular flasks for filling 20~30ml fermentation mediums, 32 DEG C of shaking table concussion trainings
Support 12~18h, 100~150r/min of rotating speed；Zymotic fluid is taken, 3500r/min centrifuges 10~15min, takes supernatant, freezes, i.e.,
；Wherein, the fermentation medium pH is 6.0, containing glucose 15~20%, yeast extract 0.1~0.5% and water 80~85%.
5. the feed as described in claim 3 or 4, it is characterised in that the acid-producing bacteria is selected from Propionibacterium, lactic acid bacteria and acetic acid
One kind in bacillus.
6. feed as claimed in claim 5, it is characterised in that the acid-producing bacteria is selected from Propionibacterium.
7. feed as claimed in claim 1 or 2, it is characterised in that the feed is also carried including Human plactnta mescenchymal stem cell
Take 0.05~1 part of thing freeze-dried powder.
8. feed as claimed in claim 7, it is characterised in that the Human plactnta mescenchymal stem cell extract freeze-drying powder by
Following steps are made：
S1, the DMEM medium culture Human plactnta mescenchymal stem cells using pH6.5~7.0, containing serum to 70~80% fusion
When, its former serum-containing medium is abandoned, cell is washed with D-Hank ' s balanced salt solutions；
S2, removal balanced salt solution, continue to cultivate Human plactnta mescenchymal stem cell, culture is extremely using the DMEM culture mediums containing serum
Cell culture medium changes colour, and collects supernatant；
S3, filtering supernatant, concentration are degerming, add freeze-dried excipient and water, freeze, produce.
9. a kind of method for preparing the feed as described in claim 1~8 is any, it is characterised in that comprise the following steps：
Lucidum spore powder is taken, is crushed, sweet potato powder, taro meal, dehydrated potato powder, alphalise starch, white fish meal, red fish meal and mould proof is added
Agent, is well mixed, and sieves, and packing is produced.
10. a kind of ganoderma lucidum soft-shelled turtle cultural method, it is characterised in that be included in and monthly give soft-shelled turtle feeding such as claim 1 in first 10~15 days
The feed that~8 any described feeds or claim 9 method are prepared, monthly gives soft-shelled turtle to feed soft-shelled turtle powder in latter 15~20 days,
Day inventory accounts for the 2~4% of soft-shelled turtle body weight, was fed through 24~36 months, goes out the pool.
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Cited By (1)
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