CN107279597A - A kind of fish protein peptide phagostimulant product - Google Patents
A kind of fish protein peptide phagostimulant product Download PDFInfo
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- CN107279597A CN107279597A CN201710562860.8A CN201710562860A CN107279597A CN 107279597 A CN107279597 A CN 107279597A CN 201710562860 A CN201710562860 A CN 201710562860A CN 107279597 A CN107279597 A CN 107279597A
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- phagostimulant
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/32—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from hydrolysates of wood or straw
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of fish protein peptide phagostimulant product, above-mentioned phagostimulant product is to be made using fish meat emulsion through enzymolysis, Maillard reaction, microfiltration of ceramic membrane and spray drying.Have the beneficial effect that：The phagostimulant fishy smell of preparation is strong, pure, lasting persistently, the peculiar smell of feed can be covered, with significant phagostimulating effect.Fish appetite is stimulated, improves feed palatability, the immunity of fish is improved, grazing rate is improved, feed residual is reduced, so as to reduce water pollution, is conducive to environmental protection.Accelerate the speed of growth of fish, improve food conversion ratio, the economic benefit at increase aquaculture family.Process is simple, and product quality stability is good, cheap, and application effect is good, with good economic benefit.
Description
Technical field
The present invention relates to technical field of biological product preparation, more particularly, to a kind of fish protein peptide phagostimulant product.
Background technology
Phagostimulant has obvious and positive biological action to the feed of animal, can improve the attractant of feed and agreeable to the taste
Property, improve food conversion ratio, and then the production performance of animal is improved comprehensively, such as increase feed intake, improve daily gain, improve meat
Quality etc..One side phagostimulant can cover the bad smell in feed by special odor, the sense organ of stimulating animal, with
Reach the purpose for promoting animal ingestion, accelerating growth of animal；On the other hand, ingested by phagostimulant induced animal, shorten and take the photograph
The food time, improve the utilization rate of feed.Phagostimulant in the market is largely flavouring agent or sweetener, and effect is more single
One, smell that can only be to animal or vision or the sense of taste etc. form single stimulation, and some flavouring agents or sweetener composition into
Divide and come from chemically synthesized product, medicament residue is easily produced in animal body, toxic side effect is obvious.
Prior art such as Authorization Notice No. is the B of CN 103734530 Chinese invention patent, discloses a kind of aquatic feeds
A variety of single phagostimulants are combined by phagostimulant, this method with the bile salt with rush digestion, the sharp liver function of liver protection, for aquatic products
Animal has good phagostimulating effect and the bile salt for promoting to add in digestion effect, but the phagostimulant expensive, improves foster
The cost grown.
The content of the invention
It is an object of the invention to provide a kind of low cost, high amino acid content, high raw meat fragrance fish protein peptide phagostimulant
The processing method of product.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：
A kind of fish protein peptide phagostimulant product, is through enzymolysis, Maillard reaction, microfiltration of ceramic membrane and spray drying using fish meat emulsion
It is made.
Enzymolysis step is stirring evenly and then adding into papain to be added water in fish meat emulsion, and addition is 600~
1700IU/g, pH be 5.5 ~ 6.8, temperature be 55~60 DEG C under conditions of, digest 4~6h.Under above-mentioned condition, proteinase activity
Height, enzymolysis speed is fast, fish meat emulsion slurry degree of hydrolysis is big, makes full use of raw material, it is to avoid the waste of protein resource.
Maillard reaction step is that the corncob water of enzymolysis liquid volume fraction 17%~21% is added first in enzymolysis liquid
Liquid, 0.4%~0.8% traditional Chinese medicine liquid of enzymolysis liquid weight, the active peptides of enzymolysis liquid weight 0.2%~0.4% are solved, then
Stirring mixing is delivered in Maillard reaction kettle, is rapidly heated to 105~115 DEG C, carry out time of Maillard reaction for 1.5~
2.5h.A series of dulcet intermediate reduction ketone of tool and volatile heterocyclic compounds are generated during Maillard reaction, greatly
The big local flavor for adding enzymolysis liquid, is improved the palatability of phagostimulant.
The amino acid sequence of active peptides is：HSHACASYSRRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRV
LHPGKLCVCVNCSR.The addition of active peptides promotes merging for Corncob hydrolysate, traditional Chinese medicine liquid and fish meat emulsion enzymolysis liquid,
Favourable condition is provided for Maillard reaction, the phagostimulant mouthfeel level of processing is more enriched.
The preparation method of Corncob hydrolysate：Take in the market to buy corncob, with pulverizer crushing maize core, cross 30 ~ 60
Mesh sieve point, and dried under the conditions of 90 ~ 110 DEG C to constant weight；By maize cob meal and 1.6%~1.8% sulfuric acid of 5 ~ 9 times of quality
Or hydrochloric acid solution mixes 20~25min of immersion, and mixed liquor is heated into 125~135 DEG C of 70 ~ 100min of hydrolysis process, to reaction
Mixture afterwards carries out suction filtration, obtains Corncob hydrolysate.Corncob contains ancient thick protein 32%, crude fat 0.4%, sugar
54.5%th, crude fibre 29.7%, mineral matter 12%, are a kind of containing the more comprehensive feed of sugared higher, nutriment, add and lure
Eat the nutritional ingredient of agent.
The preparation method of traditional Chinese medicine liquid：By weight by 100~130 parts ward off Chinese grass, 25~45 parts of Radix Codonopsis, 75~95 parts
Peppermint, 5~15 portions of Asiatic plantains, 20~30 portions of wild purple perillas, 15~20 portions of saline cistanches, 20~30 portions of Semen Cuscutaes crushed 70 ~ 110
With 600~650 parts of water 100~110min of boiling after mesh sieve, decoction is then concentrated into water content 20 ~ 50% and obtains Chinese medicines
Liquid.On the one hand the Chinese medicine of addition can improve the immunity of fish, it is to avoid fish are attacked by the disease, and on the other hand can pierce
Swash fish fast-growth.
Microfiltration of ceramic membrane step is that the liquid that the ceramic membrane for using aperture for 60-210nm is obtained to Maillard reaction is carried out
Micro-filtration, liquid is concentrated in vacuo to the 1/4 ~ 3/4 of original volume under the conditions of absolute pressure 0.01-0.02MPa, 65-75 DEG C of temperature.
Preferably, the aperture of ceramic membrane is 60~210nm in filter process, under the conditions of being somebody's turn to do, major part can be filtered out
Impurity, is easy to the intake and digestion of fish.
Compared with prior art, the advantage of the invention is that：The feed fishy smell of preparation is strong, pure, lasting persistently, can
The peculiar smell of feed is covered, with significant phagostimulating effect.Fish appetite is stimulated, improves feed palatability, the immune of fish is improved
Power, improves grazing rate, reduces feed residual, so as to reduce water pollution, is conducive to environmental protection.Accelerate the speed of growth of fish, carry
High feed conversion rate, the economic benefit at increase aquaculture family.Process is simple, and product quality stability is good, cheap,
Application effect is good, with good economic benefit.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
A kind of fish protein peptide phagostimulant product, is through enzymolysis, Maillard reaction, microfiltration of ceramic membrane and spray drying using fish meat emulsion
It is made.
A kind of processing method of fish protein peptide phagostimulant product, concrete operations are：
1）Oppress the preparation of pulp：The fish meat emulsion and water that remove the internal organs, go after the gill, cleaning, stripping and slicing and rubbing are mixed to prepare, institute
The mass ratio for stating fish meat emulsion and water is 1: 0.6, provides appropriate moisture to oppress the enzymolysis of pulp, increases the enzyme of alkali protease
Speed is solved, promotes energy transmission and the mass transfer of enzymolysis process；
2）Enzymolysis：Papain is added, addition is that every gram of flesh of fish pulp 1700IU, pH are 8, and temperature is 55 DEG C of condition
Under, 5h is digested, after enzymolysis terminates, temperature is risen into 90 DEG C of holding 20min, makes enzyme-deactivating, obtains enzymolysis liquid, under the conditions of being somebody's turn to do,
Alkaline protease activity is high, and enzymolysis speed is fast, fish meat emulsion slurry degree of hydrolysis is big, makes full use of raw material, it is to avoid protein resource
Waste；
3）U.S. rad flavouring：To step 2）Enzymolysis liquid in the Corncob hydrolysate of addition enzymolysis liquid volume fraction 20%, enzymolysis liquid
0.5% traditional Chinese medicine liquid of weight, 0.3% active peptides of enzymolysis liquid weight, stirring mixing are delivered in Maillard reaction kettle,
It is rapidly heated to 105 DEG C, the time for carrying out Maillard reaction is 1.5h, and question response is cooled to room temperature after terminating, obtains Mei Lade
Reaction solution.The addition of the active peptides promotes merging for Corncob hydrolysate, traditional Chinese medicine liquid and fish meat emulsion enzymolysis liquid, is U.S. drawing
Moral reaction provides favourable condition, the phagostimulant mouthfeel level of processing is more enriched.Generated during Maillard reaction
A series of dulcet intermediate reduction ketone of tool and volatile heterocyclic compounds, considerably increase the local flavor of enzymolysis liquid, make food calling
The palatability of agent is improved.Wherein, the preparation method of Corncob hydrolysate：Take in the market to buy corncob, use pulverizer powder
Broken corncob, crosses 50 mesh sieves point, and is dried under the conditions of 110 DEG C to constant weight, by maize cob meal and 1.6% sulphur of 8 times of quality
Acid solution mixes immersion 20min, and mixed liquor is added to 125 DEG C of hydrolysis process 100min, reacted mixture is taken out
Filter, obtains Corncob hydrolysate, and corncob contains ancient thick protein 32%, crude fat 0.4%, sugar 54.5%, crude fibre
29.7%th, mineral matter 12%, is a kind of containing the more comprehensive feed of sugared higher, nutriment, add the nutrition of phagostimulant into
Point.The preparation method of traditional Chinese medicine liquid is：By weight by 100 parts ward off Chinese grass, 25 parts of Radix Codonopsis, 75 portions of peppermints, 5 portions of Asiatic plantains,
20 parts of wild purple perillas, 15 portions of saline cistanches, 20 portions of Semen Cuscutaes crushed after 110 mesh sieves with 600 parts of min of water boiling 100, then will
Decoction is concentrated into water content 35% and obtains traditional Chinese medicine liquid, and on the one hand the Chinese medicine of addition can improve the immunity of fish, it is to avoid fish
Class is attacked by the disease, and on the other hand can stimulate fish fast-growth；
4）Centrifugation, microfiltration of ceramic membrane：Liquid but after, centrifuged using link-suspended basket centrifuge（2000 revs/min of rotating speed）Remove slag, adopt
The inorganic tubular ceramic membrane for being 200nm with membrane aperture to the circulating micro-filtration of liquid row after centrifugation, when Liquid Residue volume be about into
Feed liquid 10% when, stop operation, obtain permeate, as fish protein peptide phagostimulant product.The membrane aperture taken can be filtered out
Most of impurity, is easy to the intake and digestion of fish.
Embodiment 2：
A kind of fish protein peptide phagostimulant product, is through enzymolysis, Maillard reaction, microfiltration of ceramic membrane and spray drying using fish meat emulsion
It is made.
A kind of processing method of fish protein peptide phagostimulant product, concrete operations are：
1）Oppress the preparation of pulp：The fish meat emulsion and water that remove the internal organs, go after the gill, cleaning, stripping and slicing and rubbing are mixed to prepare, institute
The mass ratio for stating fish meat emulsion and water is 1: 1, provides appropriate moisture to oppress the enzymolysis of pulp, increases the enzymolysis of alkali protease
Speed, promotes energy transmission and the mass transfer of enzymolysis process；
2）Enzymolysis：Papain is added, addition is that every gram of flesh of fish pulp 1600IU, pH are 9, and temperature is 60 DEG C of condition
Under, 6h is digested, after enzymolysis terminates, temperature is risen into 90 DEG C of holding 20min, makes enzyme-deactivating, obtains enzymolysis liquid, under the conditions of being somebody's turn to do,
Alkaline protease activity is high, and enzymolysis speed is fast, fish meat emulsion slurry degree of hydrolysis is big, makes full use of raw material, it is to avoid protein resource
Waste；
3）U.S. rad flavouring：To step 2）Enzymolysis liquid in the Corncob hydrolysate of addition enzymolysis liquid volume fraction 18%, enzymolysis liquid
0.7% traditional Chinese medicine liquid of weight, 0.4% active peptides of enzymolysis liquid weight, stirring mixing are delivered in Maillard reaction kettle,
It is rapidly heated to 110 DEG C, the time for carrying out Maillard reaction is 2h, and question response is cooled to room temperature after terminating, obtains Mei Lade anti-
Answer liquid.The addition of the active peptides promotes merging for Corncob hydrolysate, traditional Chinese medicine liquid and fish meat emulsion enzymolysis liquid, is Mei Lade
Reaction provides favourable condition, the phagostimulant mouthfeel level of processing is more enriched.One is generated during Maillard reaction
The dulcet intermediate reduction ketone of series tool and volatile heterocyclic compounds, considerably increase the local flavor of enzymolysis liquid, make phagostimulant
Palatability be improved.Wherein, the preparation method of Corncob hydrolysate：Take in the market to buy corncob, crushed with pulverizer
Corncob, crosses 50 mesh sieves point, and is dried under the conditions of 110 DEG C to constant weight, by maize cob meal and 1.7% sulfuric acid of 8 times of quality
Solution mixes immersion 23min, and mixed liquor is added to 130 DEG C of hydrolysis process 100min, and suction filtration is carried out to reacted mixture,
Corncob hydrolysate is obtained, corncob contains ancient thick protein 32%, crude fat 0.4%, sugar 54.5%, crude fibre 29.7%, ore deposit
Material 12%, is a kind of containing the more comprehensive feed of sugared higher, nutriment, adds the nutritional ingredient of phagostimulant.Chinese medicines
The preparation method of liquid is：By weight Chinese grass, 28 parts of Radix Codonopsis, 80 parts of peppermints, 7 parts of Asiatic plantains, 23 parts of wild purples are warded off by 105 parts
Soviet Union, 17 portions of saline cistanches, 22 portions of Semen Cuscutaes crushed after 110 mesh sieves with 610 parts of water boiling 105min, then decoction is concentrated into and contained
Water 35% obtains traditional Chinese medicine liquid, and on the one hand the Chinese medicine of addition can improve the immunity of fish, it is to avoid fish are by disease
Invasion and attack, on the other hand can stimulate fish fast-growth；
4）Centrifugation, microfiltration of ceramic membrane：Liquid but after, centrifuged using link-suspended basket centrifuge（2000 revs/min of rotating speed）Remove slag, adopt
The inorganic tubular ceramic membrane for being 180nm with membrane aperture to the circulating micro-filtration of liquid row after centrifugation, when Liquid Residue volume be about into
Feed liquid 10% when, stop operation, obtain permeate, as fish protein peptide phagostimulant product.The membrane aperture taken can be filtered out
Most of impurity, is easy to the intake and digestion of fish.
Embodiment 3：
A kind of fish protein peptide phagostimulant product, is through enzymolysis, Maillard reaction, microfiltration of ceramic membrane and spray drying using fish meat emulsion
It is made.
A kind of processing method of fish protein peptide phagostimulant product, concrete operations are：
1）Oppress the preparation of pulp：The fish meat emulsion and water that remove the internal organs, go after the gill, cleaning, stripping and slicing and rubbing are mixed to prepare, institute
The mass ratio for stating fish meat emulsion and water is 1: 1.2, provides appropriate moisture to oppress the enzymolysis of pulp, increases the enzyme of alkali protease
Speed is solved, promotes energy transmission and the mass transfer of enzymolysis process；
2）Enzymolysis：Papain is added, addition is that every gram of flesh of fish pulp 1300IU, pH are 8, and temperature is 58 DEG C of condition
Under, 4h is digested, after enzymolysis terminates, temperature is risen into 90 DEG C of holding 20min, makes enzyme-deactivating, obtains enzymolysis liquid, under the conditions of being somebody's turn to do,
Alkaline protease activity is high, and enzymolysis speed is fast, fish meat emulsion slurry degree of hydrolysis is big, makes full use of raw material, it is to avoid protein resource
Waste；
3）U.S. rad flavouring：To step 2）Enzymolysis liquid in the Corncob hydrolysate of addition enzymolysis liquid volume fraction 21%, enzymolysis liquid
0.6% traditional Chinese medicine liquid of weight, 0.2% active peptides of enzymolysis liquid weight, stirring mixing are delivered in Maillard reaction kettle,
It is rapidly heated to 115 DEG C, the time for carrying out Maillard reaction is 2.5h, and question response is cooled to room temperature after terminating, obtains Mei Lade
Reaction solution.The addition of the active peptides promotes merging for Corncob hydrolysate, traditional Chinese medicine liquid and fish meat emulsion enzymolysis liquid, is U.S. drawing
Moral reaction provides favourable condition, the phagostimulant mouthfeel level of processing is more enriched.Generated during Maillard reaction
A series of dulcet intermediate reduction ketone of tool and volatile heterocyclic compounds, considerably increase the local flavor of enzymolysis liquid, make food calling
The palatability of agent is improved.Wherein, the preparation method of Corncob hydrolysate：Take in the market to buy corncob, use pulverizer powder
Broken corncob, crosses 50 mesh sieves point, and is dried under the conditions of 110 DEG C to constant weight, by maize cob meal and 1.8% sulphur of 8 times of quality
Acid solution mixes immersion 25min, and mixed liquor is added to 135 DEG C of hydrolysis process 100min, reacted mixture is taken out
Filter, obtains Corncob hydrolysate, and corncob contains ancient thick protein 32%, crude fat 0.4%, sugar 54.5%, crude fibre
29.7%th, mineral matter 12%, is a kind of containing the more comprehensive feed of sugared higher, nutriment, add the nutrition of phagostimulant into
Point.The preparation method of traditional Chinese medicine liquid is：By weight by 130 parts ward off Chinese grass, 45 parts of Radix Codonopsis, 95 portions of peppermints, 15 portions of Asiatic plantains,
30 parts of wild purple perillas, 20 portions of saline cistanches, 30 portions of Semen Cuscutaes crushed after 110 mesh sieves with 650 parts of water boiling 110min, then by medicine
Liquid is concentrated into water content 35% and obtains traditional Chinese medicine liquid, and on the one hand the Chinese medicine of addition can improve the immunity of fish, it is to avoid fish
It is attacked by the disease, on the other hand can stimulates fish fast-growth；
4）Centrifugation, microfiltration of ceramic membrane：Liquid but after, centrifuged using link-suspended basket centrifuge（2000 revs/min of rotating speed）Remove slag, use
The inorganic tubular ceramic membrane that membrane aperture is 210nm is to the circulating micro-filtration of liquid row after centrifugation, when Liquid Residue volume is about charging
Liquid 10% when, stop operation, obtain permeate, as fish protein peptide phagostimulant product.The membrane aperture taken can be filtered out greatly
Partial impurities, are easy to the intake and digestion of fish.
Phagostimulant prepared by embodiment 1~3 is mixed and fry raising with existing aquatic feeds, and the addition of phagostimulant is
The 3%~5% of aquatic feeds quality, the aquatic feeds to be mixed with phagostimulant dimethyl-β-acetic acid thetin pass through as a control group
The culture experiment of 2 months, cultivation data are as follows：
| It is fed with material | Embodiment 1 | Embodiment 2 | Embodiment 3 | Control group |
| Phagostimulant addition (%) | 3 | 5 | 4 | 5 |
| Cultured area (mu) | 1 | 1 | 1 | 1 |
| Fry quantity (only) | 600 | 600 | 600 | 600 |
| Fry average weight (g) | 12.16 | 12.83 | 12.35 | 12.56 |
| Average weight (g) | 140.37 | 150.25 | 120.68 | 110.68 |
| Survival rate (%) | 91 | 93 | 89 | 72 |
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Zhejiang Feng Yu marine organisms Products Co., Ltd
<120>A kind of fish protein peptide phagostimulant product
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 60
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Ser Tyr Ser Arg Arg Cys Gly Tyr Tyr Val
1 5 10 15
Ser Lys Ala His Cys Arg Arg Ala Arg Arg Cys Gly Tyr Tyr Val Ser
20 25 30
His Arg Gly Arg Cys Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu His
35 40 45
Pro Gly Lys Leu Cys Val Cys Val Asn Cys Ser Arg
50 55 60
Claims (7)
1. a kind of fish protein peptide phagostimulant product, it is characterised in that：The phagostimulant product is through enzymolysis, Mei La using fish meat emulsion
Moral reaction, microfiltration of ceramic membrane and spray drying are made.
2. a kind of preparation method of fish protein peptide phagostimulant product according to claim 1, it is characterised in that：Described enzyme
Solution step is stirring evenly and then adding into papain to be added water in fish meat emulsion, and addition is 600~1700IU/g, is in pH
5.5 ~ 6.8, temperature be 55~60 DEG C under conditions of, digest 4~6h.
3. a kind of preparation method of fish protein peptide phagostimulant product according to claim 1, it is characterised in that：Described U.S.
Maillard reaction step is the Corncob hydrolysate of addition enzymolysis liquid volume fraction 17%~21%, enzymolysis liquid first in enzymolysis liquid
0.4%~0.8% traditional Chinese medicine liquid of weight, the active peptides of enzymolysis liquid weight 0.2%~0.4%, are followed by stirring for mixing and send
Into Maillard reaction kettle, it is rapidly heated to 105~115 DEG C, the time for carrying out Maillard reaction is 1.5~2.5h.
4. a kind of preparation method of fish protein peptide phagostimulant product according to claim 1, it is characterised in that：Described pottery
Porcelain membrane microfiltration step is that the liquid that the ceramic membrane for using aperture for 60-210nm is obtained to Maillard reaction carries out micro-filtration, and liquid exists
The 1/4 ~ 3/4 of original volume is concentrated in vacuo under the conditions of absolute pressure 0.01-0.02MPa, 65-75 DEG C of temperature.
5. a kind of preparation method of fish protein peptide phagostimulant product according to claim 3, it is characterised in that：Described work
The amino acid sequence of property polypeptide is：HSHACASYSRRCGYYVSKAHCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCV
NCSR。
6. a kind of preparation method of fish protein peptide phagostimulant product according to claim 3, it is characterised in that：Described jade
The preparation method of rice core hydrolyzate：Take in the market to buy corncob, with pulverizer crushing maize core, cross 30 ~ 60 mesh sieves and divide, and in
Dried under the conditions of 90 ~ 110 DEG C to constant weight；By maize cob meal and 1.6%~1.8% sulfuric acid or hydrochloric acid solution of 5 ~ 9 times of quality
20~25min of immersion is mixed, mixed liquor 125~135 DEG C of 70 ~ 100min of hydrolysis process are heated to, to reacted mixture
Suction filtration is carried out, Corncob hydrolysate is obtained.
7. a kind of preparation method of fish protein peptide phagostimulant product according to claim 3, it is characterised in that：In described
The preparation method of medicine decoction：By weight Chinese grass, 25~45 parts of Radix Codonopsis, 75~95 parts of peppermints, 5~15 are warded off by 100~130 parts
Part Asiatic plantain, 20~30 parts of wild purple perillas, 15~20 portions of saline cistanches, 20~30 portions of Semen Cuscutaes crushed after 70 ~ 110 mesh sieves with
600~650 parts of water 100~110min of boiling, are then concentrated into water content 20 ~ 50% by decoction and obtain traditional Chinese medicine liquid.
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| CN201710562860.8A CN107279597A (en) | 2017-07-11 | 2017-07-11 | A kind of fish protein peptide phagostimulant product |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710562860.8A CN107279597A (en) | 2017-07-11 | 2017-07-11 | A kind of fish protein peptide phagostimulant product |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102366026A (en) * | 2011-09-30 | 2012-03-07 | 徐州联益生物科技开发有限公司 | Method for making pet food attractant |
| CN103005167A (en) * | 2012-12-12 | 2013-04-03 | 孙卫青 | Fish bone pet attractant and preparation method thereof |
| CN105851682A (en) * | 2016-04-15 | 2016-08-17 | 浙江省海洋开发研究院 | Method for processing leftovers through aquatic products to prepare aquatic feed phagostimulant |
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2017
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102366026A (en) * | 2011-09-30 | 2012-03-07 | 徐州联益生物科技开发有限公司 | Method for making pet food attractant |
| CN103005167A (en) * | 2012-12-12 | 2013-04-03 | 孙卫青 | Fish bone pet attractant and preparation method thereof |
| CN105851682A (en) * | 2016-04-15 | 2016-08-17 | 浙江省海洋开发研究院 | Method for processing leftovers through aquatic products to prepare aquatic feed phagostimulant |
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Application publication date: 20171024 |