CN107266164A - A kind of preparation method of the crop nutrient solution containing extracts of red clover - Google Patents
A kind of preparation method of the crop nutrient solution containing extracts of red clover Download PDFInfo
- Publication number
- CN107266164A CN107266164A CN201710434752.2A CN201710434752A CN107266164A CN 107266164 A CN107266164 A CN 107266164A CN 201710434752 A CN201710434752 A CN 201710434752A CN 107266164 A CN107266164 A CN 107266164A
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- CN
- China
- Prior art keywords
- red clover
- preparation
- containing extracts
- nutrition liquid
- extracts
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of preparation of extracts of red clover and application, comprise the following steps：1) appropriate milk, honey and dusty yeast are added after the cleaning of screw oil expeller axle herb, fermented 57 days；2) add 50% ethanol solution appropriate, 55 DEG C of temperature control 45 DEG C extracts 2 3h, filters to obtain clear liquid；3) step 2) clear liquid hyperfiltration treatment, collect peritoneal effluent；4) continuously add ethanol to concentration of alcohol and reach 75%, cooling is stood, and is filtrated to get filtrate；5) filtrate nanofiltration is handled, and obtains concentrate；6) appropriate amino acid, potassium dihydrogen phosphate, sucrose are compounded, it is filling to produce crop nutrient solution.Foliage-spray is remarkably improved fruit quality, especially lifts effect significantly to fruit appearance quality, with major application value.
Description
Technical field
The present invention relates to a kind of preparation method of the crop nutrient solution containing extracts of red clover, belong to fertilizer production technology
Field.
Background technology
Red clover also known as red clover, red bean grass, red clover, safflower car, clover, bur clover etc..Originate in small
Sub- West Asia and the European west and south, are most important herbage and the feed in the marine climate such as Europe, eastern United States, New Zealand area
One of, there is cultivation China south and north.Red clover herb contains a variety of flavones ingredient compound such as Biochanin As
(bionchanin A) and B (formonetin), onocerin (Formononetin), onospin (Ononin), dyestuff
Lignin (Genistein), Dai (Daidzein), daidzein, daidzin, Genistin etc., wherein with Biochanin A
With onocerin content highest.
Research shows that extracts of red clover has multiple biological activities, extensively should in medicine, health products, cosmetic field
With.Patent CN200510031097.3, which is related to a kind of pratensein and prunetin pharmaceutical composition, has prevention and treatment painstaking effort
Guard system disease and osteoporosis etc. are acted on.Patent CN201210334753.7 discloses a kind of prevention and treatment chicken ascites symptoms
Feed addictive, it is combined with trifolium pratense L. isoflavones and alfalfa saponin two kinds of active components.Patent
CN201610447032.5 is related to prepares cosmetics by extracts of red clover and other plant extract compounding, with moisturizing,
Moisturizing, skin remarkable efficacy of releiving.Red clover is few in agriculture field especially fertilizer field application report up to now.
Extracts of red clover conventional preparation techniques mainly include extraction, adsorbing separation, extraction etc., but all exist substantially scarce
Fall into.Red clover is processed as powder by patent CN200810151104.7 by kneading and cutting machine, squeezer, disintegrating machine, dryer processing
End, improves alcoholic solution extraction efficiency to a certain degree, but pretreatment procedure is complicated, and equipment investment is more.Patent CN03128286.5,
CN200410013189.4 utilizes high-temperature activation silica white or alumina adsorption extracts of red clover.Column chromatography method product is pure
Degree is higher, but adsorbance is small, and resolution factor is low, and production efficiency is not high.Patent CN201110371742.1 is to red clover crude extract
In be proportionally added into ammonium sulfate and ethanol, isoflavone like substance is mainly distributed in the upper phase containing ethanol.Aqueous two-phase extraction can be
Product purity is improved to a certain extent, but separating degree is not high, and a large amount of brine wastes can be produced.
Prepared by high-purity extract is research and development focus in recent years.Patent CN201010603892.6 is related to a kind of high-purity thorn
The preparation technology of onocerin, using ethanol water as solvent, by microwave radiation technology ultrasonic extraction, further passes through macroreticular resin
Purifying.Patent CN201310349360.8 is related to red clover is crushed after using carbon dioxide supercritical extraction prepare clover
The method of alcohol.Patent CN201410681182.3 utilize amination graphene sorption extraction extracts of red clover in flavonoids into
Point, separating rate is fast, product purity is high.Above-mentioned three kinds of technique productions costs are high, and strongly limit extracts of red clover makes
Use scope.
In a word, extracts of red clover large-scale production difficulty is higher, causes extracts of red clover production cost to occupy high
Under not, thus limit application of the extracts of red clover in wide range of areas.
The content of the invention
The invention discloses a kind of nutrition liquid and preparation method thereof of crop containing extracts of red clover, it is characterised in that including such as
Lower step：
1) screw oil expeller axle herb add water cleaning after drain, be transferred in open-top receptacle, appropriate milk, honey and dusty yeast stirring
Uniformly, pour into container, maintain 25 DEG C -40 DEG C of vessel temp, ferment 5-7 days；
2) to step 1) container 2 times of ethanol solutions of volume 50% of interior addition, 45 DEG C -55 DEG C are warming up to, 2-3h, solid-liquid is extracted
Separate to obtain clear liquid；
3) by step 2) clear liquid progress hyperfiltration treatment, collect peritoneal effluent；
4) 95% ethanol to solution ethanol concentration is continuously added in peritoneal effluent and reaches 75%, 5 DEG C -10 DEG C of temperature is controlled, it is quiet
3h-4h is put, plus 2% filter aid obtains filtrate；
5) step 4) filtrate progress nanofiltration processing, purified water top, which is washed till, to meet the requirements, and stops top and washes, collects concentrate；
6) step 5) concentrate compounding 1%-5% amino acid, 2%-3% potassium dihydrogen phosphates, 1%-1.5% sucrose, it is filling
Produce crop nutrient solution.
Further, step 1) milk, honey, dusty yeast consumption be respectively the 2%-3% of red clover, 1%-2%,
0.2%-0.3%.
Further, step 1) fermentation process holding clear air.
Further, step 3) ultrafiltration membrane aperture be 10000-30000 Dalton molecular weights.
Further, step 4) use filter aid to be mixed for perlite, diatomite, bentonitic one or more.
Further, step 5) nanofiltration membrane aperture be 300-500 Dalton molecular weights.
Further, step 5) peritoneal effluent electrical conductivity is less than 25 μ s/cm, and stopping top being washed.
Further, step 5) nanofiltration concentrate fixing fabric structure is 0.2-0.25 times of stoste.
Further, step 6) amino acid be tryptophan, methionine, glutamic acid one or more of mixing.
The present invention be also claimed the nutrient solution of crop containing extracts of red clover that is prepared using the above method and its
Application in the kinds such as apple, strawberry, grape, citrus, winter jujube, peach, cherry.
The beneficial effects of the invention are as follows：
1) present invention prepares simple production process efficiently, and zymotechnique is combined with membrane separation technique, it is ensured that product is biological
Under the premise of activity, production efficiency is greatly improved.Equipment requirement is low simultaneously, it is easy to large-scale production.
2) the use scope purposes of extracts of red clover is further enriched.This technique prepares extracts of red clover dilution
Foliage-spray fruits and vegetables, can significantly improve fruit quality afterwards, and fruit mass color is good, colouring is fast, mouthfeel is good, and this application adds red
The application on agricultural fertilizer of clover.
3) raw material sources enrich, safe green.Red clover is planted extensively as herbage in China, and deposit enriches and valency
Lattice are cheap, and extracts of red clover is naturally harmless, are the preferable fertilizer of organic fruits and vegetables plantation.
Embodiment
The principle and feature of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
Embodiment 1
1) screw oil expeller axle herb adds water and drains and take 200kg after cleaning, is transferred in open-top receptacle, addition 4kg milk, 2kg honeybees
Honey and 0.4kg dusty yeasts stir, and pour into container, maintain 30 DEG C of vessel temp, ferment 5 days；
2) to step 1) the interior addition 400L50% ethanol solutions of container, 45 DEG C are warming up to, 2-3h is extracted, separation of solid and liquid is obtained clearly
Liquid about 320L；
3) by step 2) clear liquid progress hyperfiltration treatment, ultrafiltration membrane aperture is 10000 Dalton molecular weights, collects peritoneal effluent
270L；
4) 95% ethanol to solution ethanol concentration is continuously added in peritoneal effluent and reaches 75%, 5 DEG C of temperature is controlled, 3h is stood,
Plus 2% Perlite filter obtain filtrate 250L；
5) step 4) filtrate carries out nanofiltration processing, and nanofiltration membrane aperture is 300 Dalton molecular weights, and purified water top washes, to saturating
When going out liquid electrical conductivity less than 25 μ s/cm, stop top and wash, collect concentrate 50L；
6) step 5) concentrate compounding 0.5kg amino acid (tryptophan, methionine 1:1 mixing), 1kg potassium dihydrogen phosphates,
0.5kg sucrose, it is filling to produce crop nutrient solution.
7) crop nutrient solution is diluted into 200 times to spray in strawberry fruit expanding stage, often minor tick 7 days totally 3 times, plucked previous
Week plus spray are once, it can be seen that strawberry fruit color is gorgeous, and colouring is uniform, and just, fruit quality is obviously improved fruit.
Embodiment 2
1) screw oil expeller axle herb adds water and drains and take 600kg after cleaning, is transferred in open-top receptacle, addition 18kg milk, 12kg
Honey and 1.8kg dusty yeasts stir, and pour into container, maintain 35 DEG C of vessel temp, ferment 7 days；
2) to step 1) the interior addition 1200L50% ethanol solutions of container, 50 DEG C are warming up to, 3h is extracted, separation of solid and liquid is obtained clearly
Liquid about 1000L；
3) by step 2) clear liquid progress hyperfiltration treatment, ultrafiltration membrane aperture is 20000 Dalton molecular weights, collects peritoneal effluent
730L；
4) 95% ethanol to solution ethanol concentration is continuously added in peritoneal effluent and reaches 75%, 8 DEG C of temperature is controlled, 4h is stood,
Plus 2% filter aid (diatomite and perlite 1:1 mixing) it is filtrated to get filtrate 650L；
5) step 4) filtrate carries out nanofiltration processing, and nanofiltration membrane aperture is 500 Dalton molecular weights, and purified water top washes, to saturating
When going out liquid electrical conductivity less than 25 μ s/cm, stop top and wash, collect concentrate 160L；
6) step 5) concentrate compounding 4.5kg amino acid (tryptophan, methionine, glutamic acid 1:1:1 mixing), 4.8kg phosphorus
Acid dihydride potassium, 1.6kg sucrose, it is filling to produce crop nutrient solution.
7) crop nutrient solution is diluted into 300 times to spray in cherry fruit expanding stage, often minor tick 10 days totally 3 times, before harvesting
One week plus spray are once, it can be seen that cherry fruit epidermis light, colouring is more and uniform, and fruit is compared to overall bigger than normal, the fruit product of control
Matter is good.
Embodiment 3
1) screw oil expeller axle herb adds water and drains and take 1000kg after cleaning, is transferred in open-top receptacle, addition 30kg milk, 20kg
Honey and 3kg dusty yeasts stir, and pour into container, maintain 30 DEG C of vessel temp, ferment 7 days；
2) to step 1) the interior addition 2000L50% ethanol solutions of container, 55 DEG C are warming up to, 3h is extracted, separation of solid and liquid is obtained clearly
Liquid about 1600L；
3) by step 2) clear liquid progress hyperfiltration treatment, ultrafiltration membrane aperture is 30000 Dalton molecular weights, collects peritoneal effluent
1400L；
4) 95% ethanol to solution ethanol concentration is continuously added in peritoneal effluent and reaches 75%, 10 DEG C of temperature is controlled, 4h is stood,
Plus 2% filter aid (diatomite and bentonite 1:1 mixing) it is filtrated to get filtrate 1300L；
5) step 4) filtrate carries out nanofiltration processing, and nanofiltration membrane aperture is 500 Dalton molecular weights, and purified water top washes, to saturating
When going out liquid electrical conductivity less than 25 μ s/cm, stop top and wash, collect concentrate 300L；
6) step 5) concentrate compounding 15kg amino acid (methionine, glutamic acid 1:1 mixing), 9kg potassium dihydrogen phosphates, 4kg
Sucrose, it is filling to produce crop nutrient solution.
7) crop nutrient solution is diluted into 200 times to spray in Apple expanding stage, is spaced 10 days, sprays altogether 3 times, before harvesting
Every 7 days of two weeks sprayed once, sprayed 2 times altogether.The colouring of experimental result display processing group Apple is fast, uniform, and fruit is individual just, mass color
Good, high quality fruit rate substantially increases.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (10)
1. a kind of nutrition liquid and preparation method thereof of crop containing extracts of red clover, it is characterised in that comprise the following steps：
1) screw oil expeller axle herb adds water and drained after cleaning, is transferred in open-top receptacle, appropriate milk, honey and dusty yeast stirring are equal
It is even, pour into container, maintain 25 DEG C -40 DEG C of vessel temp, ferment 5-7 days；
2) to step 1) container 2 times of ethanol solutions of volume 50% of interior addition, 45 DEG C -55 DEG C are warming up to, 2-3h, separation of solid and liquid is extracted
Obtain clear liquid；
3) by step 2) clear liquid progress hyperfiltration treatment, collect peritoneal effluent；
4) 95% ethanol to solution ethanol concentration is continuously added in peritoneal effluent and reaches 75%, 5 DEG C -10 DEG C of temperature is controlled, 3h- is stood
4h, plus 2% filter aid obtain filtrate；
5) step 4) filtrate progress nanofiltration processing, purified water top, which is washed till, to meet the requirements, and stops top and washes, collects concentrate；
6) step 5) concentrate compounding 1%-5% amino acid, 2%-3% potassium dihydrogen phosphates, 1%-1.5% sucrose, it is filling to produce
Crop nutrient solution.
2. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 1) milk, honey, dusty yeast consumption be respectively red clover 2%-3%, 1%-2%, 0.2%-0.3%.
3. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 1) fermentation process holding clear air.
4. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 3) ultrafiltration membrane aperture be 10000-30000 Dalton molecular weights.
5. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 4) use filter aid to be mixed for perlite, diatomite, bentonitic one or more.
6. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 5) nanofiltration membrane aperture be 300-500 Dalton molecular weights.
7. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 5) peritoneal effluent electrical conductivity is less than 25 μ s/cm, and stopping top being washed.
8. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 5) nanofiltration concentrate fixing fabric structure is 0.2-0.25 times of stoste.
9. a kind of crop nutrition liquid and preparation method thereof containing extracts of red clover according to claim 1, it is characterised in that
Step 6) amino acid be tryptophan, methionine, glutamic acid one or more of mixing.
What 10. a kind of preparation method according to any one of claim 1-9 was prepared makees containing extracts of red clover
Thing nutrient solution and its application in the kinds such as apple, strawberry, grape, citrus, winter jujube, peach, cherry.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434752.2A CN107266164A (en) | 2017-06-10 | 2017-06-10 | A kind of preparation method of the crop nutrient solution containing extracts of red clover |
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| CN201710434752.2A CN107266164A (en) | 2017-06-10 | 2017-06-10 | A kind of preparation method of the crop nutrient solution containing extracts of red clover |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566234A (en) * | 2018-12-29 | 2019-04-05 | 山东省潍坊市农业科学院 | A kind of ilex verticillata cultivation matrix and preparation method thereof |
| CN112843045A (en) * | 2016-07-06 | 2021-05-28 | 天津中医药大学 | Application of 3' -hydroxybiochanin A and pharmaceutical composition containing same |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004084818A3 (en) * | 2003-03-21 | 2005-02-24 | Univ California | Measurement of estrogen activity in plants and a composition therefrom |
| CN104945144A (en) * | 2015-07-01 | 2015-09-30 | 德州鑫桥农化有限公司 | Agricultural planting liquid fertilizer prepared from pure traditional Chinese medicines and plant extracting solutions |
| CN106007846A (en) * | 2016-05-17 | 2016-10-12 | 烟台固特丽生物科技股份有限公司 | Preparation method of foliage fertilizer containing enteromorpha active substances |
| CN106576545A (en) * | 2016-10-31 | 2017-04-26 | 蚌埠市金牛湾农业科技发展有限公司 | Pomegranate yield increasing planting method |
-
2017
- 2017-06-10 CN CN201710434752.2A patent/CN107266164A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004084818A3 (en) * | 2003-03-21 | 2005-02-24 | Univ California | Measurement of estrogen activity in plants and a composition therefrom |
| CN104945144A (en) * | 2015-07-01 | 2015-09-30 | 德州鑫桥农化有限公司 | Agricultural planting liquid fertilizer prepared from pure traditional Chinese medicines and plant extracting solutions |
| CN106007846A (en) * | 2016-05-17 | 2016-10-12 | 烟台固特丽生物科技股份有限公司 | Preparation method of foliage fertilizer containing enteromorpha active substances |
| CN106576545A (en) * | 2016-10-31 | 2017-04-26 | 蚌埠市金牛湾农业科技发展有限公司 | Pomegranate yield increasing planting method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112843045A (en) * | 2016-07-06 | 2021-05-28 | 天津中医药大学 | Application of 3' -hydroxybiochanin A and pharmaceutical composition containing same |
| CN109566234A (en) * | 2018-12-29 | 2019-04-05 | 山东省潍坊市农业科学院 | A kind of ilex verticillata cultivation matrix and preparation method thereof |
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Application publication date: 20171020 |