CN106007846A - Preparation method of foliage fertilizer containing enteromorpha active substances - Google Patents
Preparation method of foliage fertilizer containing enteromorpha active substances Download PDFInfo
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- CN106007846A CN106007846A CN201610332517.XA CN201610332517A CN106007846A CN 106007846 A CN106007846 A CN 106007846A CN 201610332517 A CN201610332517 A CN 201610332517A CN 106007846 A CN106007846 A CN 106007846A
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- foliage fertilizer
- entermorpha
- preparation
- active substance
- fertilizer containing
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Organic Chemistry (AREA)
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Abstract
The invention relates to a preparation method of a foliage fertilizer containing enteromorpha active substances. The preparation method comprises steps as follows: dried enteromorpha powder is added to lower alcohol with the volume being 2-6 times that of the dried enteromorpha powder to be soaked, water with the volume being 10-15 times that of the dried enteromorpha powder is added, the mixture is heated to 60-80 DEG C, thermal insulation is performed for 2-3 h, a dissolution liquid is obtained through filtration and subjected to ultrafiltration treatment, dialysate is collected and subjected to nanofiltration treatment, and a concentrated liquid is obtained; 5wt%-8wt% of monopotassium phosphate, 3wt%-5wt% of phenylalanine, 1wt%-2wt% of tryptophan and 3wt%-6wt% of glucose are added to the concentrated liquid and stirred until the components are completely dissolved, filling is performed, and the foliage fertilizer is obtained. The foliage fertilizer prepared with the method is diluted by 100-400 times and then sprayed to surfaces of leaves, the appearance quality of fruits can be remarkably improved, the foliage fertilizer has been applied to apples, strawberries, red bayberries, winter jujubes, grapes, oranges and other crops and has a good use effect, and use of the foliage fertilizer is not affected after fruit bagging.
Description
Technical field
The present invention relates to the preparation method of a kind of foliage fertilizer, particularly relate to a kind of leaf containing Entermorpha active substance
The preparation method of leaven, belongs to technical field of fertilizer production.
Background technology
In recent years, dioxy in air is caused due to a large amount of burnings of the chemical fuels such as oil and the destruction of vegetation
Change carbon content increase so that the marine green algaes such as Entermorpha breed in a large number and cause " green tide " frequency increase and
There is geographical expanded range.While how solving " green tide ", it is achieved the efficient utilization of Entermorpha, become
Hot issue for scientific research.
Chinese patent ZL201210486512.4 is directed to use with stepwise discretization method and processes sea grass polysaccharide；Patent
ZL201210398986.3 is dual degraded sea grass polysaccharide under the conditions of microwave and hydrogen peroxide；Patent
ZL200910017836.1 thermal cracking Entermorpha under 500-600 DEG C of hot conditions prepares bio oil.Above-mentioned three
Although Entermorpha can be changed into available material by kind of a method, but severe reaction conditions, do not possess on a large scale
The possibility promoted.
Fruit appearance is many important quality indexs of colour pattern fruit such as Fructus Mali pumilae, pears, Fructus Vitis viniferae etc., is also
The important embodiment that its fruit marketability is good and bad.China's cultivation of fruit tree area and total output are always situated in the world
One, but fruit quality is far from meeting international market demand, and annual fruit exit amount is only the 1% of annual production
Left and right, and export price is far below other developed countries.Promoting fruit color, improving fruit quality is
The most important thing of current fruit tree technical research.
Chinese patent ZL201310447309.0 relates to improving fruit quality with reflective membrane, and reflective membrane needs
Use to be reclaimed, takes a lot of work laborious；The sucrose of patent ZL201210129020.X use 3-8 μm ol/L is molten
Liquid smears strawberry surface, remarkably promotes Fructus Fragariae Ananssae coloring, effective just for monocrop；Patent
ZL201510590133.3, patent ZL201010596944.1 utilize anthocyanin or anthocyanidin preparation blade face
Fertilizer, promotes fruit color, and production cost is high；Patent ZL200910094749.6, patent
ZL200610109273.5, patent ZL200910220679.4 relate to the use of chelated microelement and promote fruit
Real coloring, narrow application range, it is necessary to use after fruit maturation；Patent ZL200410065334.3, specially
Profit ZL200510094393.8 relates to outer 5-ALA or GNT that execute and promotes the synthesis of Apple anthocyanidin,
But expensive starting materials, does not possess large-scale promotion and is worth.
Summary of the invention
The present invention is directed to effectively utilizing and the deficiency of fruit color technology existence of existing Entermorpha, it is provided that a kind of
Preparation method containing the foliage fertilizer of Entermorpha active substance.
The technical scheme is that
The preparation method of a kind of foliage fertilizer containing Entermorpha active substance, it is characterised in that comprise the steps:
1) Entermorpha dry powder is joined in the lower alcohol of its 2～6 times of volumes soak, after be added thereto to Entermorpha body
The water of long-pending 10～15 times, is warming up to 60～80 DEG C, is incubated 2～3 hours, filters to obtain lysate, described
Lower alcohol is the monohydric alcohol containing 1～3 carbon atom；
2) by step 1) the lysate hyperfiltration treatment of gained, collect peritoneal effluent, peritoneal effluent nanofiltration is processed dense
Contracting liquid；
3) to step 2) concentrated solution of gained adds the phenylpropyl alcohol of potassium dihydrogen phosphate, 3～the 5wt% of 5～8wt%
The glucose of the tryptophan of propylhomoserin, 1～2wt%, 3～6wt% stirs to being completely dissolved, and fill obtains foliage fertilizer.
Further, the particle diameter of described Entermorpha dry powder is 50～100 mesh.
Further, step 1) described in volume is Entermorpha volume 3～4 times of lower alcohol.
Further, step 1) in add water after be warming up to 65～70 DEG C.
Further, step 2) in the aperture of ultrafilter membrane be 10000～12000 Dalton molecular weights.
Further, step 2) in the aperture of NF membrane be 500～800 Dalton molecular weights.
The present invention is also claimed the foliage fertilizer making to prepare in aforementioned manners and this foliage fertilizer at Herba Marsileae Quadrifoliae
Really, the application on Fructus Fragariae Ananssae, Fructus Myricae rubrae, Fructus Jujubae, Fructus Vitis viniferae, Citrus.
The foliage fertilizer prepared through the method for the present invention after dilution 100～400 times in foliage-spray,
Fruit appearance quality can be significantly improved, be applied to Fructus Mali pumilae, Fructus Fragariae Ananssae, Fructus Myricae rubrae, Fructus Jujubae, Fructus Vitis viniferae, Citrus
Deng crop, using effect is good, and fruit bagging does not affect product and uses.
The invention has the beneficial effects as follows:
1) environmental benefit and social benefit are notable；Entermorpha is a kind of common large ocean chlorella, belongs to chlorella
Door Chlorophyceae Ulvales Ulvaceae, this abundant raw material source is cheap.The most since two thousand seven,
China's Huanghai Sea breaks out extensive floating Entermorpha tide for continuous 7 years, has a strong impact on view and the life in coastal cities.
This project extracts active substance as primary raw material with Entermorpha, it is provided that new utilization ways, has great
Promotional value.
2) using effect is notable, wide market；Low-molecular-weight Entermorpha polysaccharide, can significantly regulate and control chalcone
Synzyme and enzyme, namely chalcone isomerase activity, be obviously enhanced secretion and the synthesis of fruit endogenous pigment, improve fruit
Real exterior quality.This regulating effect is applicable to most of coloured fruit, and therefore the product of the present invention has extensively
Spectrum adaptability.Product uses and will not cause fruit radical response simultaneously, without accelerating the ripening effect, and any life of fruit
All can use for a long time.
3) production technology is simple, environmental protection.Production requirement configuration equipment is less, puts into the highest, is beneficial to rule
Modelling produces；Use organic reagent is less, and can efficient recovery, be difficult to environmental pollution.
Detailed description of the invention
Being described principle and the feature of the present invention below in conjunction with example, example is served only for explaining this
Invention, is not intended to limit the scope of the present invention.
Embodiment 1:
The preparation method of a kind of foliage fertilizer containing Entermorpha active substance, comprises the steps:
1) the Entermorpha dry powder 10Kg of 100 mesh is joined immersion 3 hours in the ethanol of 60L, the most wherein
The water adding 100L is warming up to 60 DEG C, is incubated 3 hours, filters to obtain active substance lysate 154L；
2) by step 1) the ultrafilter membrane ultrafiltration that lysate via hole diameter is 10000 Dalton molecular weights of gained, receive
Collection peritoneal effluent 145L, by the nanofiltration membrane treatment that peritoneal effluent via hole diameter is 500 Dalton molecular weights, pushes up wash water
Volume 500L, collects concentrated solution 15L；
3) in concentrated solution, 1kg potassium dihydrogen phosphate, 0.6kg phenylalanine, 0.2kg tryptophan, 0.9kg are added
Glucose stirs to being completely dissolved, and fill obtains foliage fertilizer；
4) by step 3) gained foliage fertilizer is diluted with water to 200 times, in Fructus Mali pumilae, grape fruit expanding stage blade face
Spraying 2～3 times, fruit appearance quality significantly improves, and bagging does not affect the use of product.
Embodiment 2:
The preparation method of a kind of foliage fertilizer containing Entermorpha active substance, comprises the steps:
1) the Entermorpha dry powder 20Kg of 50 mesh is joined in the isopropanol of 40L and soak 2 hours, then to it
The water of middle addition 300L is warming up to 70 DEG C, is incubated 2 hours, filters to obtain active substance lysate 325L；
2) by step 1) the ultrafilter membrane ultrafiltration that lysate via hole diameter is 12000 Dalton molecular weights of gained, receive
Collection peritoneal effluent 300L, by the nanofiltration membrane treatment that peritoneal effluent via hole diameter is 800 Dalton molecular weights, pushes up wash water
Volume 500L, collects concentrated solution 38L；
3) in concentrated solution, 2kg potassium dihydrogen phosphate, 1.2kg phenylalanine, 0.6kg tryptophan, 1.3kg are added
Glucose stirs to being completely dissolved, and fill obtains foliage fertilizer；
4) by step 3) gained foliage fertilizer is diluted with water to 300 times, in Fructus Fragariae Ananssae, Fructus Myricae rubrae young fruit period foliage-spray
1 time, fruit expanding period foliage-spray 1～2 times, fruit colouring is fast, and uniform coloring.
Embodiment 3:
The preparation method of a kind of foliage fertilizer containing Entermorpha active substance, comprises the steps:
1) the Entermorpha dry powder 15Kg of 80 mesh is joined immersion 3 hours in the ethanol of 45L, the most wherein
The water adding 180L is warming up to 80 DEG C, is incubated 2.5 hours, filters to obtain active substance lysate 210L；
2) by step 1) the ultrafilter membrane ultrafiltration that lysate via hole diameter is 10000 Dalton molecular weights of gained, receive
Collection peritoneal effluent 198L, by the nanofiltration membrane treatment that peritoneal effluent via hole diameter is 600 Dalton molecular weights, pushes up wash water
Volume 500L, collects concentrated solution 18L；
3) in concentrated solution add 1.5kg potassium dihydrogen phosphate, 0.7kg phenylalanine, 0.25kg tryptophan,
0.9kg glucose stirs to being completely dissolved, and fill obtains foliage fertilizer；
4) by step 3) gained foliage fertilizer is diluted with water to 200 times, in Fructus Jujubae, citrusfruit colour-change period blade face
Spray 2 times, remarkably promote fruit colouring.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all in the present invention
Spirit and principle within, any modification, equivalent substitution and improvement etc. made, should be included in this
Within bright protection domain.
Claims (8)
1. the preparation method of the foliage fertilizer containing Entermorpha active substance, it is characterised in that include as follows
Step:
1) Entermorpha dry powder is joined in the lower alcohol of its 2～6 times of volumes soak, after be added thereto to Entermorpha body
The water of long-pending 10～15 times, is warming up to 60～80 DEG C, is incubated 2～3 hours, filters to obtain lysate, described
Lower alcohol is the monohydric alcohol containing 1～3 carbon atom；
2) by step 1) the lysate hyperfiltration treatment of gained, collect peritoneal effluent, peritoneal effluent nanofiltration is processed dense
Contracting liquid；
3) to step 2) concentrated solution of gained adds the phenylpropyl alcohol of potassium dihydrogen phosphate, 3～the 5wt% of 5～8wt%
The glucose of the tryptophan of propylhomoserin, 1～2wt%, 3～6wt% stirs to being completely dissolved, and fill obtains foliage fertilizer.
The preparation method of the foliage fertilizer containing Entermorpha active substance the most according to claim 1, it is special
Levying and be, the particle diameter of described Entermorpha dry powder is 50～100 mesh.
The preparation method of the foliage fertilizer containing Entermorpha active substance the most according to claim 1, it is special
Levy and be, step 1) described in volume is Entermorpha volume 3～4 times of lower alcohol.
The preparation method of the foliage fertilizer containing Entermorpha active substance the most according to claim 1, it is special
Levy and be, described step 1) in add water after be warming up to 65～70 DEG C.
The preparation method of the foliage fertilizer containing Entermorpha active substance the most according to claim 1, it is special
Levy and be, step 2) in the aperture of ultrafilter membrane be 10000～12000 Dalton molecular weights.
The preparation method of the foliage fertilizer containing Entermorpha active substance the most according to claim 1, it is special
Levy and be, step 2) in the aperture of NF membrane be 500～800 Dalton molecular weights.
7. the foliage fertilizer prepared according to the preparation method according to any one of claim 1-6.
8. the foliage fertilizer described in claim 7 is at Fructus Mali pumilae, Fructus Fragariae Ananssae, Fructus Myricae rubrae, Fructus Jujubae, Fructus Vitis viniferae, Citrus
On application.
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| CN201610332517.XA CN106007846A (en) | 2016-05-17 | 2016-05-17 | Preparation method of foliage fertilizer containing enteromorpha active substances |
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| CN201610332517.XA CN106007846A (en) | 2016-05-17 | 2016-05-17 | Preparation method of foliage fertilizer containing enteromorpha active substances |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107266164A (en) * | 2017-06-10 | 2017-10-20 | 烟台固特丽生物科技股份有限公司 | A kind of preparation method of the crop nutrient solution containing extracts of red clover |
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| CN101485343A (en) * | 2009-03-02 | 2009-07-22 | 中国海洋大学生物工程开发有限公司 | Extraction technology of active ingredients of enteromorpha and preparation method of enteromorpha seaweed fertilizer |
| CN102696942A (en) * | 2012-05-24 | 2012-10-03 | 海南大学 | Method for preparing nutrient capsules containing marine organism polysaccharide |
| CN103304305A (en) * | 2012-03-13 | 2013-09-18 | 中国海洋大学生物工程开发有限公司 | Compound-microorganism functional enteromorpha foliar fertilizer and preparation method thereof |
| CN103771918A (en) * | 2014-01-17 | 2014-05-07 | 青岛海大生物集团有限公司 | Double-alga fertilizer and preparation method thereof |
| CN104353360A (en) * | 2014-11-11 | 2015-02-18 | 复旦大学 | Multi-stage membrane separation cascade process for stage utilization of biomass hydrothermal liquefaction liquid-phase product |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
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2016
- 2016-05-17 CN CN201610332517.XA patent/CN106007846A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485343A (en) * | 2009-03-02 | 2009-07-22 | 中国海洋大学生物工程开发有限公司 | Extraction technology of active ingredients of enteromorpha and preparation method of enteromorpha seaweed fertilizer |
| CN103304305A (en) * | 2012-03-13 | 2013-09-18 | 中国海洋大学生物工程开发有限公司 | Compound-microorganism functional enteromorpha foliar fertilizer and preparation method thereof |
| CN102696942A (en) * | 2012-05-24 | 2012-10-03 | 海南大学 | Method for preparing nutrient capsules containing marine organism polysaccharide |
| CN103771918A (en) * | 2014-01-17 | 2014-05-07 | 青岛海大生物集团有限公司 | Double-alga fertilizer and preparation method thereof |
| CN104353360A (en) * | 2014-11-11 | 2015-02-18 | 复旦大学 | Multi-stage membrane separation cascade process for stage utilization of biomass hydrothermal liquefaction liquid-phase product |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
Non-Patent Citations (1)
| Title |
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| 魏鉴腾等: "浒苔多糖的研究进展", 《海洋科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107266164A (en) * | 2017-06-10 | 2017-10-20 | 烟台固特丽生物科技股份有限公司 | A kind of preparation method of the crop nutrient solution containing extracts of red clover |
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Application publication date: 20161012 |
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