CN107253870A - Coprinus comatus culture matrix - Google Patents

Coprinus comatus culture matrix Download PDF

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Publication number
CN107253870A
CN107253870A CN201710493555.8A CN201710493555A CN107253870A CN 107253870 A CN107253870 A CN 107253870A CN 201710493555 A CN201710493555 A CN 201710493555A CN 107253870 A CN107253870 A CN 107253870A
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China
Prior art keywords
parts
culture matrix
coprinus comatus
preparation
matrix according
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CN201710493555.8A
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Chinese (zh)
Inventor
谢雨欣
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Pujiang County Meize Biological Technology Co Ltd
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Pujiang County Meize Biological Technology Co Ltd
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Priority to CN201710493555.8A priority Critical patent/CN107253870A/en
Publication of CN107253870A publication Critical patent/CN107253870A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C9/00Fertilisers containing urea or urea compounds

Abstract

The invention discloses coprinus comatus culture matrix, the composition and its parts by weight of culture matrix are:55 63 parts of stalk, 15 19 parts of weed tree sawdust, 9 12 parts of animal manure, 8 12 parts of wheat bran, 12 parts of land plaster, 12 parts of quick lime, 12 parts of urea, 12 parts of phosphate fertilizer, 0.5 0.8 parts of bio-fermentation agent, 80 90 parts of water.Have the beneficial effect that:The good permeability of the culture matrix of the present invention, retention ability is strong, and nitrogen content is sufficient, and the reserve strength of abundance, the nutritious vitamin and trace element for providing abundance for coprinus comatus comprehensively are provided for the cultivation of coprinus comatus;The present invention is used as major ingredient using agricultural crop straw, it is possible to resolve crop straw burning causes problem of environmental pollution, realizes and uses lower cost, obtains the effect of notable benefits;Preparation method simple possible of the present invention, fermentation time is greatly shortened, and operating efficiency is improved, with broader practice prospect.

Description

Coprinus comatus culture matrix
Technical field
The present invention relates to field of edible fungus culture, more particularly, to coprinus comatus culture matrix.
Background technology
Coprinus comatus is being commonly called as coprinus comatus, because of its shape such as chicken leg, and meat meat flavour is gained the name like shredded chicken, is manually to open in recent years The rare bacterium product with business potential of hair, are described as " rising star in bacterium ".Chicken leg massee fruiting bodies are medium-and-large-sized all living creatures edible mushroom, Mushroom flower bud phase cap cylinder, the later stage is bell, high 7~20 centimetres, and dipped beam is slided when cap is young, after there is calm scale or surface to have to split Line.Cap, bacterial context, the lamella stem of the tender fructification of children are white, and stem slightly up to 1~2.5 centimetre, above there is collarium.Coprinus comatus is sought Abundant, delicious flavour is supported, mouthfeel is fabulous, it is often edible to help to improve a poor appetite, digest, strengthen body immunity, with very high Nutritive value.According to analysis, fresh mushroom moisture content 92.2%;Contain 25.4 grams of crude protein in every 100 grams of dry mushrooms(Its content is the 3 of rice Times, 2 times of wheat, 2.5 times of pork, 1.2 times of beef, 0.5 times of fish, 8 times of milk), 3.3 grams of fat, total reducing sugar 58.8 Gram, 7.3 grams of fiber, 12.5 grams of ash content;Also contain 1661.93 milligrams of potassium, 34.01 milligrams of sodium, 106.7 milligrams of calcium, magnesium 191.47 Milligram, macroelement and the microgram of iron 1376 such as 634.17 milligrams of phosphorus, the microgram of copper 45.37, the microgram of zinc 92.2, the microgram of manganese 29.221, The microgram of molybdenum 0.67, the trace element such as the microgram of cobalt 0.67.Coprinus comatus is also containing 20 kinds of amino acid(Including 8 kinds of amino needed by human Acid).Coprinus comatus is a kind of medicinal fungus, and mushroom body is pure white, attractive in appearance, and meat is fine and smooth;Stir-fry and eat, boiled dinner, Baoshang boils not rotten long, mouthfeel is slided Tender, delicate fragrance is delicious, thus popular with consumers again;Flat, the effects such as the having Energy benefit taste, clearing away the heart fire and tranquillizing, control hemorrhoid with sweet slip, warp Often to eat, which is used, aid digestion, increase appetite and treatment hemorrhoid.Coprinus comatus is tasty, is of high nutritive value, and to culture Expect that adaptability is high, nearly 2 years very rapid in whole nation development.China is large agricultural country, agricultural crop straw(Such as:Peanut shell, corn Straw, corncob, wheat straw, beanstalk, straw etc.)Aboundresources, still, peasant but burn up most of stalk as fuel, and this is not Only waste of resource, and pollution environment.For it is reasonable, effectively utilize resource, improve resource conversion utilization rate, use agricultural crop straw Wood chip and cotton seed hull that current price generally goes up are substituted, the Cultivation use of coprinus comatus is used as.
Prior art such as Authorization Notice No. is the B of CN 103848700 Chinese invention patent, discloses a kind of coprinus comatus and plants Matrix and preparation method thereof is trained, it is hybridly prepared into by the component of following weight:10-30 parts of oil-tea camellia husks, bagasse 20-40 parts, ramulus mori considers 20-40 parts, 10 parts of biogas residue, 4 parts of corn flour, 2 parts of wheat bran, 2 parts of rice bran, 1 part of pulverized limestone, 1 part of land plaster to be worth doing; The cultivation matrix pH value being configured to is naturally, water content is in 60-70%.The chicken leg mushroom cultivation medium pollution rate is low, and bacterium germination is fast, hair The bacterium rod quality brought out is good, and mycelial growth is pure white, healthy and strong, dense, and growing power is strong, long speed is uniform, diseases prevention performance is good, but the cultivation The training substrate fermentation time is longer, prepares the time long.
The content of the invention
It is an object of the invention to provide a kind of good permeability, retention ability is strong, and nitrogen content is sufficient, nutritious comprehensive, is Coprinus comatus provides sufficient vitamin and micro- coprinus comatus culture matrix.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is:Coprinus comatus culture matrix, composition and Its parts by weight is:55-63 parts of stalk, 15-19 parts of weed tree sawdust, 9-12 parts of animal manure, 8-12 parts of wheat bran, 1-2 parts of land plaster, quick lime 1-2 parts, 1-2 parts of urea, 1-2 parts of phosphate fertilizer, 0.5-0.8 parts of bio-fermentation agent, 80-90 parts of water.Stalk is peanut shell or corn stalk Or rape straw or wheat straw or beanstalk or straw.Bio-fermentation agent derives from Henan Shen Yu bio tech ltd.Above-mentioned culture The good permeability of matrix, retention ability is strong, and nitrogen content is sufficient, and the reserve strength of abundance is provided for the cultivation of coprinus comatus, nutritious complete Face, the vitamin and trace element of abundance are provided for coprinus comatus.
The preparation method of coprinus comatus culture matrix, specifically includes following steps:
Formula ratio urea, phosphate fertilizer is soluble in water, produce nutrient solution;
By formula ratio stalk, weed tree sawdust, animal manure, wheat bran, land plaster, quick lime, stir to form compound, quick lime is not only Play a part of the certain miscellaneous bacteria of killing and pest and disease damage, increase outside the mineral element such as calcareous, topmost effect is exactly regulation training The pH value of nutriment, usual cotton seed hulls is replaced as the major ingredient of cultivating chicken leg mushroom using agricultural crop straw matrix, not only can be with Agricultural crop straw is turned waste into wealth, solve crop straw burning and cause problem of environmental pollution, lower cost is used while also achieving, obtain The effect of notable benefits;
Nutrient solution and formula ratio bio-fermentation agent are added in compound, active peptides are added, addition is bio-fermentation agent The 0.005-0.008% of weight, stirs, and all siccatives is all suctioned moisture, sealing, and ferment 36- at 25-30 DEG C 40h, the amino acid sequences of active peptides is HSHACKLGAAKCYLCRVLHPGKLCVCNCSK, the active peptide rich in growth because Son, can promote absorption and utilization of the microorganism to nutritional ingredient, promote the growth of microorganism, accelerate the reproduction speed of bacterium, Shorten CDC, promote the breeding of mushroom in leavening, shorten fermentation time;
Compost moisture content is adjusted to water to pack and seal after 60-70%, the every bag of 1-1.2Kg that feeds in terms of siccative, by packed training Nutriment sterilizes 3-5h at 120-130 DEG C, produces coprinus comatus culture matrix, the good permeability of the culture matrix, and retention ability is strong, contains Nitrogen quantity is sufficient, and the reserve strength of abundance, the nutritious dimension life for providing abundance for coprinus comatus comprehensively are provided for the cultivation of coprinus comatus Element and trace element, while without chemicals in the sterilization process of culture matrix, and take pure natural substance and thing Reason method is sterilized, and be ensure that safety while yield is ensured, is made the coprinus comatus produced will not be by culture matrix Pollution, has really accomplished free of contamination organic food.
Compared with prior art, the advantage of the invention is that:The good permeability of the culture matrix of the present invention, retention ability is strong, Nitrogen content is sufficient, and the reserve strength of abundance, the nutritious dimension for providing abundance for coprinus comatus comprehensively are provided for the cultivation of coprinus comatus Raw element and trace element, while without chemicals in the sterilization process of culture matrix, and take pure natural substance and Physical method is sterilized, and be ensure that safety while yield is ensured, is made the coprinus comatus produced will not be by culture matrix Pollution, really accomplished free of contamination organic food;The present invention replaces cottonseed generally using agricultural crop straw matrix Shell can not only make agricultural crop straw turn waste into wealth as the major ingredient of cultivating chicken leg mushroom, solve crop straw burning and cause environmental pollution Problem, lower cost is used while also achieving, and obtains the effect of notable benefits;The present invention materials extensively, low cost, high efficiency and Material-saving, preparation method simple possible, fermentation time is greatly shortened, and operating efficiency is improved, with broader practice prospect.
Embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
Coprinus comatus culture matrix, composition and its parts by weight are:60 parts of stalk, 17 parts of weed tree sawdust, 10 parts of animal manure, 10 parts of wheat bran, stone 1.5 parts of cream powder, 1.4 parts of quick lime, 1.5 parts of urea, 1.3 parts of phosphate fertilizer, 0.6 part of bio-fermentation agent, 88 parts of water.Stalk is peanut shell Or corn stalk or rape straw or wheat straw or beanstalk or straw.
The preparation method of coprinus comatus culture matrix, specifically includes following steps:
1)Formula ratio urea, phosphate fertilizer is soluble in water, produce nutrient solution;
2)By formula ratio stalk, weed tree sawdust, animal manure, wheat bran, land plaster, quick lime, stir to form compound, quick lime is not Only play a part of the certain miscellaneous bacteria of killing and pest and disease damage, increase outside the mineral element such as calcareous, topmost effect is exactly to adjust The pH value of compost, using the cotton seed hulls of agricultural crop straw matrix replacement generally as the major ingredient of cultivating chicken leg mushroom, not only may be used So that agricultural crop straw is turned waste into wealth, solve crop straw burning and cause problem of environmental pollution, use lower cost while also achieving, obtain Obtain the effect of notable benefits;
3)Nutrient solution and formula ratio bio-fermentation agent are added in compound, active peptides are added, addition is biofermentation The 0.006% of agent weight, stirs, and all siccatives is all suctioned moisture, sealing, and ferment 37h, active peptides at 28 DEG C Amino acid sequence be HSHACKLGAAKCYLCRVLHPGKLCVCNCSK, the active peptide be rich in growth factor, can promote micro- Biological absorption and utilization to nutritional ingredient, promotes the growth of microorganism, accelerates the reproduction speed of bacterium, shortens cell division week Phase, promote the breeding of mushroom in leavening, shorten fermentation time;
4)Pack and seal after Compost moisture content is adjusted into 65% with water, the every bag of 1Kg that fed in terms of siccative, by packed compost Sterilize 4h at 125 DEG C, produces coprinus comatus culture matrix, the good permeability of the culture matrix, and retention ability is strong, and nitrogen content is sufficient, Provide the reserve strength of abundance for the cultivation of coprinus comatus, it is nutritious comprehensively, provide sufficient vitamin and micro for coprinus comatus Element, while without chemicals in the sterilization process of culture matrix, and take pure natural substance and physical method to enter Row sterilizing, ensure that safety while yield is ensured, the coprinus comatus produced is not polluted by culture matrix, really Accomplished free of contamination organic food.
Embodiment 2:
Coprinus comatus culture matrix, composition and its parts by weight are:22 parts of peanut shell, 36 parts of corn stalk, 16 parts of weed tree sawdust, animal manure 11 Part, 11 parts of wheat bran, 1.5 parts of land plaster, 1.8 parts of quick lime, 1.3 parts of urea, 1.5 parts of phosphate fertilizer, 0.7 part of bio-fermentation agent, water 90 Part.
The preparation method of coprinus comatus culture matrix, specifically includes following steps:
Formula ratio urea, phosphate fertilizer is soluble in water, produce nutrient solution;
By formula ratio peanut shell, corn stalk, weed tree sawdust, animal manure, wheat bran, land plaster, quick lime, stir to form compound, Quick lime not only acts as the certain miscellaneous bacteria of killing and pest and disease damage, increased outside the effect of mineral element such as calcareous, topmost effect The pH value of compost is exactly adjusted, cotton seed hulls generally is replaced as the master of cultivating chicken leg mushroom using agricultural crop straw matrix Material, can not only agricultural crop straw is turned waste into wealth, solve crop straw burning cause problem of environmental pollution, while also achieve with compared with Low cost, obtains the effect of notable benefits;
Nutrient solution and formula ratio bio-fermentation agent are added in compound, active peptides are added, addition is bio-fermentation agent The 0.007% of weight, stirs, and all siccatives is all suctioned moisture, sealing, and ferment 38h at 30 DEG C, active peptides Amino acid sequence is HSHACKLGAAKCYLCRVLHPGKLCVCNCSK, and the active peptide is rich in growth factor, can promote micro- life Absorption and utilization of the thing to nutritional ingredient, promote the growth of microorganism, accelerate the reproduction speed of bacterium, shorten cell division week Phase, promote the breeding of mushroom in leavening, shorten fermentation time;
Pack and seal after Compost moisture content is adjusted into 65% with water, the every bag of 1.2Kg that fed in terms of siccative, by packed compost Sterilize 5h at 128 DEG C, produces coprinus comatus culture matrix, the good permeability of the culture matrix, and retention ability is strong, and nitrogen content is sufficient, Provide the reserve strength of abundance for the cultivation of coprinus comatus, it is nutritious comprehensively, provide sufficient vitamin and micro for coprinus comatus Element, while without chemicals in the sterilization process of culture matrix, and take pure natural substance and physical method to enter Row sterilizing, ensure that safety while yield is ensured, the coprinus comatus produced is not polluted by culture matrix, really Accomplished free of contamination organic food.
Embodiment 3:
Coprinus comatus culture matrix, composition and its parts by weight are:60 parts of wheat straw, 17 parts of weed tree sawdust, 11 parts of animal manure, 11 parts of wheat bran, stone 1.5 parts of cream powder, 1.7 parts of quick lime, 1.8 parts of urea, 2 parts of phosphate fertilizer, 0.7 part of bio-fermentation agent, 85 parts of water.
The preparation method of coprinus comatus culture matrix, specifically includes following steps:Formula ratio urea, phosphate fertilizer is soluble in water, produce Nutrient solution;By formula ratio stalk, weed tree sawdust, animal manure, wheat bran, land plaster, quick lime, stir to form compound;By nutrition Liquid and formula ratio bio-fermentation agent are added in compound, add active peptides, and addition is bio-fermentation agent weight 0.008%, stir, seal, ferment 38h at 26 DEG C;Pack and seal after Compost moisture content is adjusted into 68% with water, often Bag is fed 1.2Kg in terms of siccative, and packed compost is sterilized 3h at 130 DEG C, coprinus comatus culture matrix is produced.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Pujiang County Mei Ze bio tech ltd
<120>Coprinus comatus culture matrix
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 30
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Lys Leu Gly Ala Ala Lys Cys Tyr Leu Cys Arg
1 5 10 15
Val Leu His Pro Gly Lys Leu Cys Val Cys Asn Cys Ser Lys
20 25 30

Claims (8)

1. coprinus comatus culture matrix, it is characterised in that:The composition and its parts by weight of described culture matrix be:55-63 parts of stalk, 15-19 parts of weed tree sawdust, 9-12 parts of animal manure, 8-12 parts of wheat bran, 1-2 parts of land plaster, 1-2 parts of quick lime, 1-2 parts of urea, phosphate fertilizer 1-2 Part, 0.5-0.8 parts of bio-fermentation agent, 80-90 parts of water.
2. coprinus comatus culture matrix according to claim 1, it is characterised in that:Described stalk is peanut shell or corn stalk Or rape straw or wheat straw or beanstalk or straw.
3. coprinus comatus culture matrix according to claim 1, it is characterised in that:The preparation method tool of described culture matrix Body comprises the following steps:
1)Formula ratio urea, phosphate fertilizer is soluble in water, produce nutrient solution;
2)By formula ratio stalk, weed tree sawdust, animal manure, wheat bran, land plaster, quick lime, stir to form compound;
3)Nutrient solution and formula ratio bio-fermentation agent are added in compound, active peptides is added, stirs, is made all dry Material all suctions moisture, seals, fermentation;
4)Water use regulation Compost moisture content, packs and seals, sterilizing, produces coprinus comatus culture matrix.
4. the preparation method of coprinus comatus culture matrix according to claim 3, it is characterised in that:It is living in described step 3 Property polypeptide addition be bio-fermentation agent weight 0.005-0.008%, its amino acid sequence is HSHACKLGAAKCYLCRVLHPGKLCVCNCSK。
5. the preparation method of coprinus comatus culture matrix according to claim 3, it is characterised in that:Sent out in described step 3 Ferment temperature is 25-30 DEG C, and the time is 36-40h.
6. the preparation method of coprinus comatus culture matrix according to claim 3, it is characterised in that:Used in described step 4 Compost moisture content is adjusted to 60-70% by water.
7. the preparation method of coprinus comatus culture matrix according to claim 3, it is characterised in that:It is every in described step 4 Bag charging 1-1.2Kg in terms of siccative.
8. the preparation method of coprinus comatus culture matrix according to claim 3, it is characterised in that:Gone out in described step 4 Bacterium temperature is 120-130 DEG C, and the time is 3-5h.
CN201710493555.8A 2017-06-26 2017-06-26 Coprinus comatus culture matrix Pending CN107253870A (en)

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CN110199780A (en) * 2019-06-20 2019-09-06 中国科学院东北地理与农业生态研究所 A kind of cultivation matrix with broomcorn straw part substitution sawdust mushroom cultivation

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Publication number Priority date Publication date Assignee Title
CN110199780A (en) * 2019-06-20 2019-09-06 中国科学院东北地理与农业生态研究所 A kind of cultivation matrix with broomcorn straw part substitution sawdust mushroom cultivation

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Application publication date: 20171017