CN107365181A - Hedgehog hydnum mushroom culture medium and preparation method thereof - Google Patents

Hedgehog hydnum mushroom culture medium and preparation method thereof Download PDF

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Publication number
CN107365181A
CN107365181A CN201710469260.7A CN201710469260A CN107365181A CN 107365181 A CN107365181 A CN 107365181A CN 201710469260 A CN201710469260 A CN 201710469260A CN 107365181 A CN107365181 A CN 107365181A
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parts
culture medium
preparation
hericium erinaceus
described step
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CN201710469260.7A
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Chinese (zh)
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李健
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Lanxi Goth Biological Technology Co Ltd
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Lanxi Goth Biological Technology Co Ltd
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Priority to CN201710469260.7A priority Critical patent/CN107365181A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

Abstract

The invention discloses hedgehog hydnum mushroom culture medium and preparation method thereof, the composition and its parts by weight of culture medium are:47 52 parts of stalk, 15 19 parts of weed tree sawdust, 9 12 parts of animal manure, 12 parts of leavening, 35 parts of bean dregs, 5 10 parts of wheat bran, 12 parts of calcium superphosphate, 12 parts of ammonium hydrogen carbonate, 12 parts of land plaster, 12 parts of quick lime, 0.5 0.8 parts of urea.Have the beneficial effect that:The good permeability of the culture medium of the present invention, retention ability is strong, and nitrogen content is sufficient, provides the reserve strength of abundance for the cultivation of Hericium erinaceus, suitable Hericium erinaceus growth, raw material sources are extensive, and cost is low, realize the ecological balance, improve economic benefit, realize the reasonable utilization of resource;The present invention uses secondary fermentation so that culture medium quality is high, and pest and disease damage is few, and mycelia is energetic after inoculation, and uniformity coefficient is good, increases the yield of Hericium erinaceus, has broader practice prospect.

Description

Hedgehog hydnum mushroom culture medium and preparation method thereof
Technical field
The present invention relates to fungus growing technique field, more particularly, to hedgehog hydnum mushroom culture medium and preparation method thereof.
Background technology
Hericium erinaceus(Scientific name:Hericium erinaceus), be Chinese tradition luxury dish, meat tenderness, it is savory, delicious can Mouthful, it is four big famous dishes(Hedgehog hydnum, bear's paw, sea cucumber, shark's fin)One of.There is the title of " mountain delicacy hedgehog hydnum, seafood delights bird's nest ".The nutrition of Hericium erinaceus Composition is very high, is qualified high protein, low-fat food, is also rich in various vitamins and inorganic salts in addition.Hericium erinaceus has Improve a poor appetite, strengthen barrier of gastric mucosa function, improve the effect such as lymphocyte transformation rate, lifting leucocyte, therefore human body can be made Improve the immunocompetence to disease.Hedgehog hydnum or good nourishing food, there is good efficacy to neurasthenia, digestive tract ulcer. In screening anticancer medicine, it is found that it has obvious anticancer function to skin, muscle cancerous swelling.Hericium erinaceus is a kind of domestomycetes, is had Stronger decomposes lignin, the ability of cellulose, can use under the agricultural byproducts such as cotton seed hulls, corncob, weed tree sawdust, bagasse, straw Heel needs abundant carbon source and nitrogen source as culturing raw material, the growth of Hericium erinaceus, and especially nitrogen source is abundanter, and mycelial growth is got over Good, yield is also higher, but nowadays, " bacterium-woods " contradiction, cotton seed hulls class cost of material continuous rise, therefore each production Unit is all attached great importance to look for cheap and good-quality alternative materials.It is organic that agricultural crop straw contains abundant cellulose and lignin etc. Thing, it is the good material for cultivating edible mushroom.The edible mushrooms such as Hericium erinaceus, flat mushroom, agaric, mushroom, asparagus are cultivated using rice wheat straw, Higher economic benefit can be obtained, its culture medium can also make high-quality organic fertilizer.China is large agricultural country, agricultural crop straw (Such as:Peanut shell, corn stalk, corncob, wheat straw, beanstalk, straw etc.)Aboundresources, still, peasant but make most of stalk Burnt up for fuel, this not only waste of resource, and pollute environment.Edible mushroom is produced using stalk, it is possible to achieve the resource of stalk Change, commercialization, for cleaning and protecting rural environment, promote increasing peasant income, if resource-efficient and environment friendly society, Promoting The Construction of New Countryside is all of great practical significance.
Prior art such as Authorization Notice No. is the B of CN 103641557 Chinese invention patent, discloses a kind of utilize to day The method that certain herbaceous plants with big flowers accessory substance makes hericium erinaceus culture material, the planting material is in accessory substance-sunflower disk of sunflower, Sunflower Stalk, sunflower Raw material is wanted based on seed shell and sunflower seeds grouts, it is auxiliary to be aided with cotton seed hulls, wheat bran, calcium superphosphate, potassium dihydrogen phosphate and land plaster etc. Material, the water content of planting material is 63%-65%.The planting material reasonable raw material proportioning, promote planting material physical behavior complementation and The complementation of nutrient, make bacterium material good permeability, water-retaining property strong, hericium mycelium growth is vigorous, but planting material fermentation is not thorough, Hericium erinaceus can be made to produce putrefactive phenomenon.
The content of the invention
It is an object of the invention to provide a kind of good permeability, retention ability is strong, and nitrogen content is sufficient, suitable Hericium erinaceus growth, former Expect wide material sources, the low hedgehog hydnum mushroom culture medium of cost and preparation method thereof.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is:Hedgehog hydnum mushroom culture medium, composition and its Parts by weight are:Stalk 47-52 parts, weed tree sawdust 15-19 parts, animal manure 9-12 parts, leavening 1-2 parts, bean dregs 3-5 parts, wheat bran 5-10 Part, calcium superphosphate 1-2 parts, ammonium hydrogen carbonate 1-2 parts, land plaster 1-2 parts, quick lime 1-2 parts, urea 0.5-0.8 parts.Leavening comes Come from Henan Shen Yu bio tech ltd.The good permeability of above-mentioned culture medium, retention ability is strong, and nitrogen content is sufficient, is hedgehog hydnum The cultivation of mushroom provides the reserve strength of abundance, and very suitable Hericium erinaceus growth, raw material sources are extensive, and cost is low, realize the ecological balance, carry High economic benefit, while the reasonable utilization of resource is realized to a certain extent.
The preparation method of hedgehog hydnum mushroom culture medium, specifically includes following steps:
By formula ratio stalk, weed tree sawdust, animal manure, land plaster, quick lime, stir to form compound, per turning one in 5-7 days It is secondary, stack 20-25d and carry out first time fermentation, produce one time fermentation product, the quick lime in the step can not only be killed certain Miscellaneous bacteria and pest and disease damage, increase outside the effect of mineral element such as calcareous, most important effect is exactly the pH value for adjusting compost, is used Agricultural crop straw matrix replace generally by the use of cotton seed hulls be used as Hericium erinaceus culture major ingredient, can not only make agricultural crop straw become give up For treasured, solve crop straw burning and cause problem of environmental pollution, while also achieve with lower cost, obtain the effect of notable benefits;
Leavening is added in one time fermentation product, adds bioactive peptide, addition is the 0.008- of bio-fermentation agent weight 0.01%, stir, add water to make the water content of one time fermentation product be 65-70%, seal, ferment 30-33h at 23-28 DEG C, Secondary fermentation material is produced, the amino acid sequence of bioactive peptide is HSHACLHPGCVNCYRCSR, and the bioactive peptide is rich in growth The factor, absorption and utilization of the microorganism to nutritional ingredient can be promoted, promote the growth of microorganism, accelerate the breeding speed of bacterium Degree, shorten CDC, promote the breeding of mushroom in leavening, shorten fermentation time, by secondary fermentation so that culture Matrix amount is high, and pest and disease damage is few, and mycelia is energetic after inoculation, and uniformity coefficient is good, increases the yield of Hericium erinaceus;
Formula ratio bean dregs, wheat bran, calcium superphosphate, ammonium hydrogen carbonate, urea are added in secondary fermentation product, is well mixed, uses water Compost moisture content is adjusted to 60-70%, acid-base modifier is added and make it that the pH of cultivation matrix is 3-5, pack and seal, every bag Fed 0.2-0.3Kg in terms of siccative, and packed compost is sterilized 3-5h at 120-130 DEG C, produces hedgehog hydnum mushroom culture medium, the training The good permeability of base is supported, retention ability is strong, and nitrogen content is sufficient, and the reserve strength of abundance, very suitable hedgehog hydnum are provided for the cultivation of Hericium erinaceus Mushroom is grown, while chemicals are not added in the sterilization process of culture matrix, and takes pure natural substance and physical method Sterilized, ensure that safety while yield is ensured, the Hericium erinaceus for making to produce will not be polluted by culture matrix, very Positive has accomplished free of contamination organic food.
Compared with prior art, the advantage of the invention is that:1)The good permeability of the culture medium of the present invention, retention ability is strong, Nitrogen content is sufficient, and the reserve strength of abundance is provided for the cultivation of Hericium erinaceus, and very suitable Hericium erinaceus growth, raw material sources are extensive, cost It is low, the ecological balance is realized, improves economic benefit, while realizes the reasonable utilization of resource to a certain extent;2)Using agriculture Crop material matrix replace generally by the use of cotton seed hulls be used as Hericium erinaceus culture major ingredient, can not only make agricultural crop straw change give up into Treasured, solve crop straw burning and cause problem of environmental pollution, while also achieve with lower cost, obtain the effect of notable benefits;3) The present invention uses secondary fermentation so that culture medium quality is high, and pest and disease damage is few, and mycelia is energetic after inoculation, and uniformity coefficient is good, increase The yield of Hericium erinaceus, there is broader practice prospect.
Embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
Hedgehog hydnum mushroom culture medium, composition and its parts by weight are:50 parts of stalk, weed tree sawdust 185-19 parts, 10 parts of animal manure, leavening 1.5 Part, 4 parts of bean dregs, 8 parts of wheat bran, 1.4 parts of calcium superphosphate, 1.2 parts of ammonium hydrogen carbonate, 1 part of land plaster, 2 parts of quick lime, 0.6 part of urea. The good permeability of above-mentioned culture medium, retention ability is strong, and nitrogen content is sufficient, and the reserve strength of abundance is provided for the cultivation of Hericium erinaceus, very suitable Suitable Hericium erinaceus growth, raw material sources are extensive, and cost is low, realize the ecological balance, improve economic benefit, while to a certain extent Realize the reasonable utilization of resource.
The preparation method of hedgehog hydnum mushroom culture medium, specifically includes following steps:
1)By formula ratio stalk, weed tree sawdust, animal manure, land plaster, quick lime, stir to form compound, it is high to pile 70cm Ji Dui, every turning in 6 days once, stack 23d, produce one time fermentation product, the quick lime in the step can not only be killed certain Miscellaneous bacteria and pest and disease damage, increase outside the effect of mineral element such as calcareous, most important effect is exactly the pH value for adjusting compost, is used Agricultural crop straw matrix replace generally by the use of cotton seed hulls be used as Hericium erinaceus culture major ingredient, can not only make agricultural crop straw become give up For treasured, solve crop straw burning and cause problem of environmental pollution, while also achieve with lower cost, obtain the effect of notable benefits;
2)Leavening is added in one time fermentation product, adds bioactive peptide, addition is bio-fermentation agent weight 0.009%, stir, add water to make the water content of one time fermentation product be 68%, sealed with covered rearing with plastic film, issued at 25 DEG C Ferment 32h, secondary fermentation material is produced, the amino acid sequence of bioactive peptide is HSHACLHPGCVNCYRCSR, and the bioactive peptide is rich Containing growth factor, absorption and utilization of the microorganism to nutritional ingredient can be promoted, promote the growth of microorganism, accelerate the numerous of bacterium Speed is grown, shortens CDC, promotes the breeding of mushroom in leavening, shortens fermentation time, by secondary fermentation so that Culture medium quality is high, and pest and disease damage is few, and mycelia is energetic after inoculation, and uniformity coefficient is good, increases the yield of Hericium erinaceus;
3)Formula ratio bean dregs, wheat bran, calcium superphosphate, ammonium hydrogen carbonate, urea are added in secondary fermentation product, are well mixed, is used Compost moisture content is adjusted to 68% by water, is added acid-base modifier and make it that the pH of cultivation matrix is 4, loads 15 × 55cm low pressure Polyethylene plastic bag simultaneously seals, every bag in terms of siccative feed 0.25Kg, by packed compost at 125 DEG C autoclaving 4h, i.e., Hedgehog hydnum mushroom culture medium, the good permeability of the culture medium, retention ability is strong, and nitrogen content is sufficient, provides and fills for the cultivation of Hericium erinaceus The reserve strength of foot, very suitable Hericium erinaceus growth, while chemicals are not added in the sterilization process of culture matrix, and take pure Natural materials and physical method are sterilized, and safety is ensure that while yield is ensured, make the Hericium erinaceus produced will not be by To the pollution of culture matrix, free of contamination organic food is really accomplished.
Embodiment 2:
Hedgehog hydnum mushroom culture medium, composition and its parts by weight are:51 parts of stalk, 17 parts of weed tree sawdust, 11 parts of animal manure, 2 parts of leavening, bean dregs 4 parts, 7 parts of wheat bran, 2 parts of calcium superphosphate, 1 part of ammonium hydrogen carbonate, 1.5 parts of land plaster, 1.8 parts of quick lime, 0.7 part of urea.Above-mentioned training The good permeability of base is supported, retention ability is strong, and nitrogen content is sufficient, and the reserve strength of abundance, very suitable hedgehog hydnum are provided for the cultivation of Hericium erinaceus Mushroom grows, and raw material sources are extensive, and cost is low, realize the ecological balance, improve economic benefit, while realize to a certain extent The reasonable utilization of resource.
The preparation method of hedgehog hydnum mushroom culture medium, specifically includes following steps:
By formula ratio stalk, weed tree sawdust, animal manure, land plaster, quick lime, stir to form compound, per turning one in 5-7 days It is secondary, stack 24d and carry out first time fermentation, produce one time fermentation product, the quick lime in the step can not only be killed certain miscellaneous Bacterium and pest and disease damage, increase outside the effect of mineral element such as calcareous, most important effect is exactly the pH value for adjusting compost, using agriculture Crop material matrix replace generally by the use of cotton seed hulls be used as Hericium erinaceus culture major ingredient, can not only make agricultural crop straw change give up into Treasured, solve crop straw burning and cause problem of environmental pollution, while also achieve with lower cost, obtain the effect of notable benefits;
Leavening is added in one time fermentation product, adds bioactive peptide, addition is the 0.01% of bio-fermentation agent weight, Stir, add water to make the water content of one time fermentation product be 70%, sealing, ferment 30h at 26 DEG C, produces secondary fermentation thing Matter, the amino acid sequence of bioactive peptide is HSHACLHPGCVNCYRCSR, and the bioactive peptide is rich in growth factor, can promoted micro- Absorption and utilization of the biology to nutritional ingredient, promote the growth of microorganism, accelerate the reproduction speed of bacterium, shorten cell division week Phase, promote the breeding of mushroom in leavening, shorten fermentation time, by secondary fermentation so that culture medium quality is high, pest and disease damage Few, mycelia is energetic after inoculation, and uniformity coefficient is good, increases the yield of Hericium erinaceus;
Formula ratio bean dregs, wheat bran, calcium superphosphate, ammonium hydrogen carbonate, urea are added in secondary fermentation product, is well mixed, uses water Compost moisture content is adjusted to 68%, acid-base modifier is added and make it that the pH of cultivation matrix is 3, pack and seal, every bag with siccative Meter charging 0.2Kg, packed compost is sterilized 4h at 126 DEG C, produces hedgehog hydnum mushroom culture medium, the good permeability of the culture medium, Retention ability is strong, and nitrogen content is sufficient, and the reserve strength of abundance is provided for the cultivation of Hericium erinaceus, very suitable Hericium erinaceus growth, while is training Support in the sterilization process of matrix and do not add chemicals, and take pure natural substance and physical method to be sterilized, ensureing Safety is ensure that while yield, the Hericium erinaceus for making to produce will not be polluted by culture matrix, really accomplish without dirt The organic food of dye.
Embodiment 3:
Hedgehog hydnum mushroom culture medium, composition and its parts by weight are:50 parts of stalk, 16 parts of weed tree sawdust, 10 parts of animal manure, 1 part of leavening, bean dregs 4 parts, 7 parts of wheat bran, 1.5 parts of calcium superphosphate, 1.5 parts of ammonium hydrogen carbonate, 2 parts of land plaster, 1.6 parts of quick lime, 0.7 part of urea.
The preparation method of hedgehog hydnum mushroom culture medium, specifically includes following steps:By formula ratio stalk, weed tree sawdust, animal manure, gypsum Powder, quick lime, stir to form compound, every turning in 5 days once, stacks 25d, produces one time fermentation product;By leavening Add in one time fermentation product, add bioactive peptide, addition is the 0.01% of bio-fermentation agent weight, stirs, adds water The water content for making one time fermentation product is 65%, and sealing, ferment 30-33h at 23-28 DEG C, produces secondary fermentation material;It will match somebody with somebody Side's amount bean dregs, wheat bran, calcium superphosphate, ammonium hydrogen carbonate, urea are added in secondary fermentation product, are well mixed, with water by compost Water content is adjusted to 68%, adds acid-base modifier and make it that the pH of cultivation matrix is 5, packs and seal, sterilize, produce Hericium erinaceus training Support base.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>The Gothic Bioisystech Co., Ltd of Lanxi City
<120>Hedgehog hydnum mushroom culture medium and preparation method thereof
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 18
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Leu His Pro Gly Cys Val Asn Cys Tyr Arg Cys
1 5 10 15
Ser Arg

Claims (9)

1. hedgehog hydnum mushroom culture medium, it is characterised in that:The composition and its parts by weight of described culture medium be:Stalk 47-52 parts, weedtree Consider 15-19 parts, animal manure 9-12 parts, leavening 1-2 parts, bean dregs 3-5 parts, wheat bran 5-10 parts, calcium superphosphate 1-2 parts, ammonium hydrogen carbonate to be worth doing 1-2 parts, land plaster 1-2 parts, quick lime 1-2 parts, urea 0.5-0.8 parts.
2. the preparation method of hedgehog hydnum mushroom culture medium, it is characterised in that:Described preparation method specifically includes following steps:
1)By formula ratio stalk, weed tree sawdust, animal manure, land plaster, quick lime, stir to form compound, stack and carry out first Secondary fermentation, produce one time fermentation product;
2)Leavening is added in one time fermentation product, bioactive peptide is added, stirs, regulation water content is to seal, hair Ferment, produce secondary fermentation material;
3)Formula ratio bean dregs, wheat bran, calcium superphosphate, ammonium hydrogen carbonate, urea are added in secondary fermentation product, are well mixed, is adjusted Moisture content and pH, pack and seal, sterilize, produce hedgehog hydnum mushroom culture medium.
3. the preparation method of coprinus comatus culture matrix according to claim 2, it is characterised in that:One in described step 1 During secondary fermentation per turning in 5-7 days once, 20-25d is stacked.
4. the preparation method of coprinus comatus culture matrix according to claim 2, it is characterised in that:It is living in described step 2 Property small peptide addition be bio-fermentation agent weight 0.008-0.01%, its amino acid sequence is HSHACLHPGCVNCYRCSR.
5. the preparation method of coprinus comatus culture matrix according to claim 2, it is characterised in that:Adjusted in described step 2 The water content for saving one time fermentation product is 65-70%.
6. the preparation method of coprinus comatus culture matrix according to claim 2, it is characterised in that:Sent out in described step 2 Ferment temperature is 23-28 DEG C, time 30-33h.
7. the preparation method of coprinus comatus culture matrix according to claim 2, it is characterised in that:Trained in described step 3 Nutriment water content is adjusted to 60-70%, and pH is adjusted to as 3-5.
8. the preparation method of coprinus comatus culture matrix according to claim 2, it is characterised in that:It is every in described step 3 Bag charging 0.2-0.3Kg in terms of siccative.
9. the preparation method of coprinus comatus culture matrix according to claim 2, it is characterised in that:Gone out in described step 3 Bacterium temperature is 120-130 DEG C, time 3-5h.
CN201710469260.7A 2017-06-20 2017-06-20 Hedgehog hydnum mushroom culture medium and preparation method thereof Pending CN107365181A (en)

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CN109220554A (en) * 2018-11-19 2019-01-18 遵义市播州区铃经纬香菇种植有限公司 A kind of monkey mushroom culture substrate formula
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CN109122028A (en) * 2018-09-30 2019-01-04 安徽神州生态农业发展有限公司 A kind of planting technique of Hericium erinaceus
CN109220554A (en) * 2018-11-19 2019-01-18 遵义市播州区铃经纬香菇种植有限公司 A kind of monkey mushroom culture substrate formula
CN109429901A (en) * 2018-12-14 2019-03-08 马山县山山农业科技有限公司 The preparation method of edible fungus production increasing culture medium

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Application publication date: 20171121