CN107217010A - A kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium - Google Patents
A kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium Download PDFInfo
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- CN107217010A CN107217010A CN201610834887.3A CN201610834887A CN107217010A CN 107217010 A CN107217010 A CN 107217010A CN 201610834887 A CN201610834887 A CN 201610834887A CN 107217010 A CN107217010 A CN 107217010A
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- bacterium
- coronoid process
- dissipate capsule
- process dissipate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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Abstract
The invention discloses a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium, comprise the following steps：(1) it is separated by filtration；(2) wash；(3) dried in low-temperature vacuum environment；(4) coronoid process dissipate capsule bacterium powder will be obtained after dried thalline crushing, sieving；(5) it is mixed to get mixed liquor；(6) desalination and initial concentration processing；(7) cryogenic vacuum evaporation and concentration processing obtains no bacterium concentrate.The utilization that the present invention can be lost in endobacillary benefit materials and the metabolin being secreted into outside thalline, has been obviously improved the utilization rate of coronoid process dissipate capsule bacterium, has saved resource, enhanced product competitiveness.
Description
Technical field
The invention belongs to the product treatment processes field of the coronoid process dissipate capsule bacterium of Liquid Culture, more particularly to a kind of Liquid Culture
The bacterium solution separation method of coronoid process dissipate capsule bacterium.
Background technology
Coronoid process dissipate capsule bacterium is also known as " golden flower " bacterium as a kind of fungi of Eurotium, because it is main in Fu tea fermentation process
Produce a large amount of golden yellow cleistotheciums and gain the name.
The exclusive quality of other kind tea is different from as Fu-brick tea, " golden flower " bacterium produces in the metabolism of Fu-brick tea fermentation process
Thing contains a variety of components beneficial to human body, likewise, a large amount of materials beneficial to human body can be also produced in liquid incubation,
Research shows that coronoid process dissipate capsule bacterium and its metabolite contain nearly all human body essential amino acid, and also anti-aging, drop
The effects such as fat, raising body immunity.It is focus on carrying out purification utilization to the thalline of coronoid process dissipate capsule bacterium in existing technology more,
But also have many beneficial metabolites to discharge coronoid process dissipate capsule bacterium outside bacterial in the metabolic process of coronoid process dissipate capsule bacterium and
It is present in nutrient solution, accordingly, it would be desirable to develop a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium, is dissipated in purification coronoid process
Also rationally utilized to being present in benefit materials in nutrient solution during capsule bacterium thalline, to improve the utilization of coronoid process dissipate capsule bacterium
Rate.
The content of the invention
In order to overcome defect of the prior art, there is provided a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium.
The present invention is realized by following proposal：
A kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium, comprises the following steps：(1) liquid of coronoid process dissipate capsule bacterium
Cultivating system is filtered in gnotobasis by 200-600 mesh filter mediums, and thalline and nutrient solution are separated；
(2) with the bacterium obtained equivalent to the purified water rapid washing steps (1) of 0.5-1 times of nutrient solution volume in step (1)
Body；
(3) thalline obtained by step (2) is dried in low-temperature vacuum environment；
(4) coronoid process dissipate capsule bacterium powder will be obtained after dried thalline crushing, sieving；
(5) gained washing lotion is mixed to get mixing after being washed after being separated in step (1) in the nutrient solution and step (2) of gained
Liquid；
(6) mixed liquor obtained by step (5) is subjected to desalination by ultrafiltration and initial concentration is handled, obtain that liquid is concentrated by ultrafiltration；
(7) liquid will be concentrated by ultrafiltration obtained by step (6) and carries out cryogenic vacuum evaporation and concentration processing, obtain cultivating concentrated liquor,
Then cold filtration is degerming obtains no bacterium concentrate；
In step (1), the filter medium is filter cloth or stainless (steel) wire.
It is that temperature is 30-60 DEG C in step (3) described low-temperature vacuum environment, air pressure is 0.01-0.09MPa.
The screen cloth of sieving is 100-250 mesh in the step (4).
In the step (7) cryogenic vacuum evaporation and concentration processing be temperature be 30-60 DEG C, air pressure is 0.01-
Carried out under conditions of 0.09MPa.
The inventive method has the beneficial effect that：
The present invention is that a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium solves liquid from coronoid process dissipate capsule bacterium
The Related Technical Issues for separating nutrient solution and thalline in cultivating system, and thalline has been carried out to be dried to powder, to separation
Gained washing lotion carries out concentration after nutrient solution and washing obtained by afterwards, thus can be to endobacillary benefit materials and secretion
The utilization that metabolin outside to thalline is not lost in, has been obviously improved the utilization rate of coronoid process dissipate capsule bacterium, has saved resource, enhancing
Product competitiveness.
Embodiment
With reference to specific embodiment, the present invention is further described：
A kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium, comprises the following steps：(1) liquid of coronoid process dissipate capsule bacterium
Cultivating system is filtered in gnotobasis by 200-600 mesh filter mediums, and thalline and nutrient solution are separated；
(2) with the bacterium obtained equivalent to the purified water rapid washing steps (1) of 0.5-1 times of nutrient solution volume in step (1)
Body；
(3) thalline obtained by step (2) is dried in low-temperature vacuum environment；
(4) coronoid process dissipate capsule bacterium powder will be obtained after dried thalline crushing, sieving；
In actual use, coronoid process dissipate capsule bacterium powder obtained by step (4) can be added is pressed into again in Fu-brick tea instant tea powder
Mould assembly Fu buccal tablets；It can be sprayed onto in Fu-brick tea, loose tea or other tealeaves and be fermented as strain；Addition can be used as
Agent is added in food, health products or other products；Can by gained coronoid process dissipate capsule bacterium powder extraction active ingredient therein,
Further to utilize.
(5) gained washing lotion is mixed to get mixing after being washed after being separated in step (1) in the nutrient solution and step (2) of gained
Liquid；
(6) mixed liquor obtained by step (5) is subjected to desalination by ultrafiltration and initial concentration is handled, obtain that liquid is concentrated by ultrafiltration；
(7) liquid will be concentrated by ultrafiltration obtained by step (6) and carries out cryogenic vacuum evaporation and concentration processing, obtain cultivating concentrated liquor,
Then cold filtration is degerming obtains no bacterium concentrate；
Step (7) obtains culture concentrated liquor and can be used for preparing functional beverage；It can be added in white wine and work(is made
Can sexual health wine；Solubility coronoid process dissipate capsule bacterium metabolite powder product can be made and adds as additive by freeze-drying
It is added in other food or health products.
In step (1), the filter medium is filter cloth or stainless (steel) wire.
It is that temperature is 30-60 DEG C in step (3) described low-temperature vacuum environment, air pressure is 0.01-0.09MPa.
The screen cloth of sieving is 100-250 mesh in the step (4).
In the step (7) cryogenic vacuum evaporation and concentration processing be temperature be 30-60 DEG C, air pressure is 0.01-
Carried out under conditions of 0.09MPa.
The present invention is that a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium solves liquid from coronoid process dissipate capsule bacterium
The Related Technical Issues for separating nutrient solution and thalline in cultivating system, and thalline has been carried out to be dried to powder, to separation
Gained washing lotion carries out concentration after nutrient solution and washing obtained by afterwards, thus can be to endobacillary benefit materials and secretion
The utilization that metabolin outside to thalline is not lost in, has been obviously improved the utilization rate of coronoid process dissipate capsule bacterium, has saved resource, enhancing
Product competitiveness.
Although having done more detailed elaboration to technical scheme and having enumerated, it will be appreciated that for ability
For field technique personnel, modification is made to above-described embodiment or equivalent alternative solution is used, this is to those skilled in the art
It is it is clear that these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the present invention for member
Claimed scope.
Claims (5)
1. a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium, it is characterised in that comprise the following steps：(1) coronoid process dissipates
The Liquid Culture system of capsule bacterium is filtered in gnotobasis by 200-600 mesh filter mediums, by thalline and nutrient solution point
From；
(2) with the thalline obtained equivalent to the purified water rapid washing steps (1) of 0.5-1 times of nutrient solution volume in step (1)；
(3) thalline obtained by step (2) is dried in low-temperature vacuum environment；
(4) coronoid process dissipate capsule bacterium powder will be obtained after dried thalline crushing, sieving；
(5) gained washing lotion is mixed to get mixed liquor after being washed after being separated in step (1) in the nutrient solution and step (2) of gained；
(6) mixed liquor obtained by step (5) is subjected to desalination by ultrafiltration and initial concentration is handled, obtain that liquid is concentrated by ultrafiltration；
(7) liquid will be concentrated by ultrafiltration obtained by step (6) and carries out cryogenic vacuum evaporation and concentration processing, obtain cultivating concentrated liquor, then
Cold filtration is degerming to obtain no bacterium concentrate.
2. a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium according to claim 1, it is characterised in that：In step
Suddenly in (1), the filter medium is filter cloth or stainless (steel) wire.
3. a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium according to claim 1, it is characterised in that：In step
Suddenly (3) described low-temperature vacuum environment is that temperature is 30-60 DEG C, and air pressure is 0.01-0.09MPa.
4. a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium according to claim 1, it is characterised in that：Institute
The screen cloth for stating sieving in step (4) is 100-250 mesh.
5. a kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium according to claim 1, it is characterised in that：Institute
It in temperature is 30-60 DEG C to state in step (7) processing of cryogenic vacuum evaporation and concentration to be, air pressure is to enter under conditions of 0.01-0.09MPa
OK.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610834887.3A CN107217010A (en) | 2016-09-01 | 2016-09-01 | A kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium |
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| CN201610834887.3A CN107217010A (en) | 2016-09-01 | 2016-09-01 | A kind of bacterium solution separation method of Liquid Culture coronoid process dissipate capsule bacterium |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006095964A1 (en) * | 2005-03-08 | 2006-09-14 | Hyun Jin Jin | Method for abstract of liquid extract from chlorella |
| CN102120964A (en) * | 2010-12-21 | 2011-07-13 | 湖南城市学院 | Preparation method of eurotium cristatum spore suspension |
| CN103865810A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Method for culturing golden flower fungus powder by utilizing tea leftovers |
-
2016
- 2016-09-01 CN CN201610834887.3A patent/CN107217010A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006095964A1 (en) * | 2005-03-08 | 2006-09-14 | Hyun Jin Jin | Method for abstract of liquid extract from chlorella |
| CN102120964A (en) * | 2010-12-21 | 2011-07-13 | 湖南城市学院 | Preparation method of eurotium cristatum spore suspension |
| CN103865810A (en) * | 2014-03-26 | 2014-06-18 | 湖南城市学院 | Method for culturing golden flower fungus powder by utilizing tea leftovers |
Non-Patent Citations (1)
| Title |
|---|
| 王冰 等: "冠突散囊菌的营养作用研究进展", 《添加剂世界》 * |
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