CN107216496B - 一种可控氨基含量的壳聚糖材料及其制备方法 - Google Patents

一种可控氨基含量的壳聚糖材料及其制备方法 Download PDF

Info

Publication number
CN107216496B
CN107216496B CN201710447589.3A CN201710447589A CN107216496B CN 107216496 B CN107216496 B CN 107216496B CN 201710447589 A CN201710447589 A CN 201710447589A CN 107216496 B CN107216496 B CN 107216496B
Authority
CN
China
Prior art keywords
chitosan
solution
content
amino
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710447589.3A
Other languages
English (en)
Other versions
CN107216496A (zh
Inventor
徐永祥
张学慧
韩建民
袁慎坡
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Peking University School of Stomatology
Original Assignee
Peking University School of Stomatology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peking University School of Stomatology filed Critical Peking University School of Stomatology
Priority to CN201710447589.3A priority Critical patent/CN107216496B/zh
Publication of CN107216496A publication Critical patent/CN107216496A/zh
Application granted granted Critical
Publication of CN107216496B publication Critical patent/CN107216496B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
    • A61L26/0023—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0061—Use of materials characterised by their function or physical properties
    • A61L26/0085—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/02—Inorganic materials
    • A61L27/12—Phosphorus-containing materials, e.g. apatite
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
    • C08J5/18—Manufacture of films or sheets
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
    • C08J9/28—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a liquid phase from a macromolecular composition or article, e.g. drying of coagulum
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
    • C08J9/36—After-treatment
    • C08J9/40—Impregnation
    • C08J9/405—Impregnation with polymerisable compounds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00—Use of inorganic substances as compounding ingredients
    • C08K3/16—Halogen-containing compounds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00—Use of inorganic substances as compounding ingredients
    • C08K3/32—Phosphorus-containing compounds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K5/00—Use of organic ingredients
    • C08K5/04—Oxygen-containing compounds
    • C08K5/07—Aldehydes; Ketones
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F1/00—General methods for the manufacture of artificial filaments or the like
    • D01F1/02—Addition of substances to the spinning solution or to the melt
    • D01F1/10—Other agents for modifying properties
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F9/00—Artificial filaments or the like of other substances; Manufacture thereof; Apparatus specially adapted for the manufacture of carbon filaments
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
    • C08J2405/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00—Use of inorganic substances as compounding ingredients
    • C08K3/32—Phosphorus-containing compounds
    • C08K2003/321—Phosphates
    • C08K2003/325—Calcium, strontium or barium phosphate
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00—Polymer mixtures characterised by other features
    • C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
    • C08L2205/025—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Materials Engineering (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Manufacturing & Machinery (AREA)
  • Inorganic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Dispersion Chemistry (AREA)
  • Materials For Medical Uses (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

本发明涉及一种可控氨基含量的壳聚糖材料及其制备方法，其解决了现有方法及材料难以控制未反应氨基的含量及无法用于大块材料的制备的技术问题，其为壳聚糖、化学改性壳聚糖及其与其它有机或无机材料的复合物。本发明同时提供了其制备方法。本发明可用于医用修复材料的制备领域。

Description

一种可控氨基含量的壳聚糖材料及其制备方法
技术领域
本发明涉及医用修复材料方法技术领域，特别涉及一种可控氨基含量的壳聚糖材料及其制备方法。
背景技术
壳聚糖是一种半合成高分子氨基多糖，具有独特的化学结构，主要存在于动植物虾蟹壳中，由于对人体无毒、无刺激、无致敏、生物可降解等特性，是广泛应用的一类生物降解材料并且已广泛应用于生物医用材料领域如生物组织工程、药物释放载体和医用敷料方面等。
壳聚糖随其分子中含氨基数量的增多，其氨基特性越显著，这正是其独特性质的所在，由此奠定了壳聚糖的许多生物学特性及加工特性的基础。壳聚糖中氨基的正电荷和细菌细胞膜上负电荷的相互作用，使细胞内的蛋白酶和其他成分泄漏，从而达到抗菌、杀菌作用。此外，壳聚糖所携带的氨基正电荷能主动吸引带负电的血小板凝聚并促使红血球聚集。高度聚集的血小板破裂后，加快启动凝血反应，加速凝血酶、血清素以及血小板活化因子二磷酸腺苷、血栓素等凝血因子释放与活化，使血纤维胶原蛋白快速聚合，共同形成凝块，同时诱导红血球细胞聚集，形成血栓封堵创面，达到止血效果。
目前，壳聚糖的材料主要利用氨基进行交联的方式制备。制备过程中难以控制未反应氨基的含量，影响了壳聚糖材料的物理机械性能和生物学性能，也断绝了进一步修饰改性的可能。虽然，已有利用交联液的pH一定程度上可以改变壳聚糖微纳米颗粒的氨基含量，但是该方法无法用于大块材料如组织工程支架和敷料等的制备【Mi,F.L.；Shyu,S.S.；Lee,S.T.；Wong,T.B.,Kinetic study of chitosan‐tripolyphosphate complexreaction and acid‐resistive properties of the chitosan‐tripolyphosphate gelbeads prepared by in‐liquid curing method.Journal of Polymer Science Part BPolymer Physics 2015,37(14),1551-1564.】。
发明内容
本发明就是为了解决现有方法及材料难以控制未反应氨基的含量及无法用于大块材料的制备的技术问题，提供一种可以控制未反应氨基的含量且能应用于组织工程支架和敷料等大块材料的可控氨基含量的壳聚糖材料及其制备方法。
为此，本发明提供一种可控氨基含量的壳聚糖材料，其为壳聚糖、化学改性壳聚糖及其与其它有机或无机材料的复合物。化学改性壳聚糖是指通过有机合成方法结合其它基团，但是不改变化学物基本骨架结构的壳聚糖化合物。即，首先制备壳聚糖支架，然后在壳聚糖支架上通过有机合成方法结合其它基团。合成过程中，保持了壳聚糖支架的结构不受破坏。
优选的，有机材料是指聚乳酸、聚己内酯、聚酰胺、透明质酸、胶原、蛋白质合物。
优选的，无机材料是指碳酸钙、磷酸钙、羟基磷灰石或生物活性玻璃。
本发明同时提供一种制备可控氨基含量的壳聚糖材料的方法，其包括以下步骤：(1)将壳聚糖材料溶解到酸性溶液中，然后成型；(2)将成型壳聚糖浸入交联溶液中，交联成型；利用溶剂洗去未交联物质等，得到最终材料。
优选的，酸性溶液为甲酸、乙酸、盐酸、醋酸水溶液，所述溶液的重量百分比浓度为0.1-10％。
优选的，步骤(1)中的成型是指成为一维纤维、二维膜和三维海绵，成型方法为纺丝、流延或冻干。
优选的，步骤(2)中交联溶液至少包括一种盐和一种壳聚糖交联剂；所述盐是指具有高于15g/100ml的盐离子浓度的盐，所述盐包括卤素盐中的一种或多种的复合；所述壳聚糖交联剂包括磷酸钠、戊二醛和京尼平的一种或多种复合。
优选的，步骤(3)中溶剂为去离子水。
本发明利用壳聚糖溶解度的离子强度相关性的原理，利用离子强度维持壳聚糖材料的形态，同时保护一部分氨基不与交联剂反应，从而得到可控氨基含量的壳聚糖材料。
附图说明
图1是根据本发明的壳聚糖海绵材料制备方法的示意性流程图和原理图；
图2是根据本发明的壳聚糖材料吸水前后的实物照片和吸水溶胀率变化；使用不同交联剂溶液(A)1％(w/w)TPP,(B)3M NaCl+1％(w/w)TPP,(C)6M NaCl+1(w/w)％TPP,(D)3M NaCl+0.25(w/w)％TPP,(E)3M NaCl+2％(w/w)TPP,(F)3M NaCl+6(w/w)％TPP交联后样品，吸水前后的示意图以及对溶胀率的影响；
图3是根据本发明的方法制备的壳聚糖材料中氨基含量的变化；利用Ninhydrinassays方法测试样品中氨基的含量；如图所示，随着氯化钠浓度(CNaCl)提高，或者磷酸三钠(CTPP)浓度降低，交联纤维中的自由(未交联)氨基含量提高。
图4是根据本发明的方法制备的壳聚糖材料的氨基含量和性能的关系；样品中氨基含量与储能模量(G’)、耗能模量(G”)和弹性模量(E)的关系；
图5是根据本发明的方法制备的壳聚糖材料的细胞粘附性能；样品中氨基含量与骨髓干细胞在上面的粘附增长情况。
具体实施方式
本发明提供了一种可控氨基含量的壳聚糖材料及其制备方法和应用，下面结合附图和具体实施方式对本发明做进一步说明。
实施例1
壳聚糖的制备方法参考公告号为103665191B、申请号为01107664.X的中国发明专利进行，简述如下：对鲜虾躯壳或鲜蟹腿筛选精处理，经过三酸三碱处理，脱除虾躯壳或蟹腿的蛋白质、脂肪、色素和钙质等，制备不同脱乙酰度的壳聚糖。称取1.0g壳聚糖粉末，其中壳聚糖的脱乙酰度为85％，粘度为116cp.s。将壳聚糖溶于100mL 1％(v/v)醋酸水溶液中，配制得到1.0％(w/v)的壳聚糖溶液。
利用湿法纺丝【周家村,林亮,付晓.纯壳聚糖纤维关键技术研究及其产业化[J].高科技纤维与应用,2012,37(3):5-10.】和静电纺丝【孙康,王丽平.壳聚糖静电纺纳米纤维的制备和特点[J].应用化学,2011,28(2):123-130.】的方法制备壳聚糖纤维。
将壳聚糖纤维浸入(氯化钠+京尼平)的交联液中，搅拌过夜。用去离子水清洗样品数次，洗去残余的氯化钠和京尼平。干燥后，得到一维壳聚糖纤维。
通过调节溶液中氯化钠和磷酸三钠的浓度，调节氨基含量。随着氯化钠浓度提高，或者磷酸三钠浓度降低，氨基含量提高。
实施例2
称取1.5g壳聚糖粉末，其中壳聚糖的脱乙酰度为90％，粘度为85cp.s。取纳米羟基磷灰石0.15g，超声分散到100mL水溶液。加入1.5g壳聚糖粉末，其中壳聚糖的脱乙酰度为90％，粘度为85cp.s。搅拌下，加入2ml冰醋酸，配制得到的壳聚糖溶液。
将壳聚糖溶液成膜，然后浸入(氯化钾+戊二醛)的交联液中，搅拌过夜。用去离子水清洗样品数次，洗去残余的氯化钾+戊二醛。干燥后，得到二维壳聚糖膜。
通过调节溶液中氯化钾和戊二醛的浓度，调节氨基含量。随着氯化钾浓度提高，或者戊二醛浓度降低，氨基含量提高。
实施例3
称取2.0g透明酸钠和壳聚糖，其中壳聚糖的脱乙酰度为95％，粘度为204cp.s。溶于100mL 1％(v/v)醋酸水溶液中，配制得到2.0％(w/v)的壳聚糖溶液。然后将溶液倒在模具中，于-20℃冰箱中进行预冷冻。将冻结后的溶液取出后，放置于-20℃的饱和氯化钠水溶液中浸泡48h，得到海绵。
将得到的海绵从饱和氯化钠水溶液中取出，浸入(氯化钠+磷酸三钠)的交联液中，搅拌过夜。用去离子水清洗样品数次，洗去残余的氯化钠和磷酸三钠。然后冷冻干燥，即得到一定氨基含量的多孔壳聚糖海绵。
通过调节溶液中氯化钾和磷酸三钠的浓度，调节氨基含量。随着氯化钾浓度提高，或者磷酸三钠浓度降低，氨基含量提高。
参见图5，在支架上培养牙髓干细胞，可以明显看到，随着自由氨基含量的增加，有利于细胞的粘附增殖。

Claims (4)

1.一种制备可控氨基含量的壳聚糖材料的方法，其特征是包括以下步骤：
(1)将壳聚糖材料溶解到酸性溶液中，然后成型；
(2)将成型壳聚糖浸入交联溶液中，交联成型；所述交联溶液至少包括一种盐和一种壳聚糖交联剂；所述盐为浓度为3M或6M的氯化钠；所述壳聚糖交联剂为质量百分比浓度为0.25％、1％、2％或6％的磷酸钠；
(3)利用溶剂洗去未交联物质等，得到最终材料；
所述壳聚糖材料为壳聚糖、化学改性壳聚糖及其与其它有机或无机材料的复合物；
所述有机材料是指聚乳酸、聚己内酯、聚酰胺、透明质酸、胶原、蛋白质合物；
所述无机材料是指碳酸钙、磷酸钙、羟基磷灰石或生物活性玻璃。
2.根据权利要求1所述的制备可控氨基含量的壳聚糖材料的方法，其特征在于所述酸性溶液为甲酸、乙酸、盐酸、醋酸水溶液，所述溶液的重量百分比浓度为0.1-10％。
3.根据权利要求1所述的制备可控氨基含量的壳聚糖材料的方法，其特征在于所述步骤(1)中的成型是指成为一维纤维、二维膜和三维海绵，成型方法为纺丝、流延或冻干。
4.根据权利要求1所述的制备可控氨基含量的壳聚糖材料的方法，其特征在于所述步骤(3)中溶剂为去离子水。
CN201710447589.3A 2017-06-14 2017-06-14 一种可控氨基含量的壳聚糖材料及其制备方法 Active CN107216496B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710447589.3A CN107216496B (zh) 2017-06-14 2017-06-14 一种可控氨基含量的壳聚糖材料及其制备方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710447589.3A CN107216496B (zh) 2017-06-14 2017-06-14 一种可控氨基含量的壳聚糖材料及其制备方法

Publications (2)

Publication Number Publication Date
CN107216496A CN107216496A (zh) 2017-09-29
CN107216496B true CN107216496B (zh) 2020-11-06

Family

ID=59947508

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710447589.3A Active CN107216496B (zh) 2017-06-14 2017-06-14 一种可控氨基含量的壳聚糖材料及其制备方法

Country Status (1)

Country Link
CN (1) CN107216496B (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108339153A (zh) * 2018-02-06 2018-07-31 北京大学口腔医学院 一种3d打印多孔组织工程支架复合材料及其制备方法
CN110124635A (zh) * 2019-05-17 2019-08-16 南京工业大学 一种脂肪族聚酰胺-胺聚合物接枝壳聚糖染料吸附剂及其制备方法
CN113186649B (zh) * 2021-04-30 2022-11-08 河北宁纺集团有限责任公司 一种多彩抗菌纳米纤维膜及其制备方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106633213A (zh) * 2016-12-15 2017-05-10 李华玲 一种壳聚糖凝胶的制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101081314B (zh) * 2007-06-27 2010-12-08 浙江大学 一种复合羟基磷灰石的胶原/壳聚糖支架的制备方法
CN102274192B (zh) * 2011-08-09 2013-08-28 山西医科大学第一医院 一种羧甲基壳聚糖载药微球及其制备方法
TWI477295B (zh) * 2013-07-04 2015-03-21 Benq Materials Corp 止血敷料及其製造方法
CN105170107B (zh) * 2015-10-16 2017-11-07 西安科技大学 一种绿色重金属捕集剂的制备方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106633213A (zh) * 2016-12-15 2017-05-10 李华玲 一种壳聚糖凝胶的制备方法

Also Published As

Publication number Publication date
CN107216496A (zh) 2017-09-29

Similar Documents

Publication Publication Date Title
Meng et al. Chitosan/alginate/hyaluronic acid polyelectrolyte composite sponges crosslinked with genipin for wound dressing application
Kanimozhi et al. Development of biomimetic hybrid porous scaffold of chitosan/polyvinyl alcohol/carboxymethyl cellulose by freeze-dried and salt leached technique
Sionkowska et al. Modification of collagen and chitosan mixtures by the addition of tannic acid
JP4463702B2 (ja) 伸縮性コラーゲン成形体、その製造方法および用途
CN107216496B (zh) 一种可控氨基含量的壳聚糖材料及其制备方法
Balitaan et al. Innovation inspired by nature: Biocompatible self-healing injectable hydrogels based on modified-β-chitin for wound healing
JP4753525B2 (ja) 組織再生用基材、移植用材料及びその製法
Saarai et al. A comparative study of crosslinked sodium alginate/gelatin hydrogels for wound dressing
CN105597156B (zh) 水凝胶及其制备方法和应用
CN112156222B (zh) 一种止血抗菌促愈合的冷冻凝胶海绵制备方法
Correia et al. Chitosan scaffolds with a shape memory effect induced by hydration
Yan et al. Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance
CN105142399A (zh) 生物相容的和生物可吸收的衍生的壳聚糖组合物
JP7494395B2 (ja) 筋肉幹細胞培養用架橋型ハイドロゲルの調製方法及びその使用
CN113769156A (zh) 兼具止血和创面修复的杂化纤维海绵及其制备方法
CN105617459A (zh) 一种纳米聚吡咯甲壳素神经导管的制备方法
Kangjian et al. Preparation and characterization of collagen–chitosan–chondroitin sulfate composite membranes
CN110003499A (zh) 一种抗菌水凝胶及其制备方法
Feng et al. A hemostatic keratin/alginate hydrogel scaffold with methylene blue mediated antimicrobial photodynamic therapy
Yin et al. Synthesis of chemical cross-linked gelatin hydrogel reinforced with cellulose nanocrystals (CNC)
JP2012001859A (ja) コラーゲン・キトサン複合繊維状多孔体及びその製造方法
CN110028685A (zh) 一种丝素/壳聚糖/多孔氧化石墨烯复合三维支架的制备方法
Sionkowska et al. Preparation and characterization of new materials based on silk fibroin, chitosan and nanohydroxyapatite
CN109897194B (zh) 一种仿昆虫表皮结构的高强度水凝胶制备方法
CN110613864A (zh) 一种改性竹纤维增强的壳聚糖基多孔材料及其制备方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant