CN107212002A - A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants and its application - Google Patents

A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants and its application Download PDF

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Publication number
CN107212002A
CN107212002A CN201710425346.XA CN201710425346A CN107212002A CN 107212002 A CN107212002 A CN 107212002A CN 201710425346 A CN201710425346 A CN 201710425346A CN 107212002 A CN107212002 A CN 107212002A
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China
Prior art keywords
wide
plants
spectrum
preventing
viral diseases
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CN201710425346.XA
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Inventor
吴元华
董蕴琦
宋影
赵秀香
安梦楠
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Priority to CN201710425346.XA priority Critical patent/CN107212002A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/541,3-Diazines; Hydrogenated 1,3-diazines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of wide-spectrum bactericide for preventing and treating viral diseases of plants, two kinds of pharmaceutical agent combinations of cytosintetidemycin and amino-oligosaccharide are prepared, with advantages below:Cytosintetidemycin and amino-oligosaccharide have induction, prevention and treatment multiple action after mixing by a certain percentage, its synergistic effect is notable;Diseases prevention spectrum is wide, has preventive and therapeutic effect to the multiple diseases of a variety of host plants;Induce plant to produce immunity, the defence capability of plant itself can be made full use of, reduce the risk for using and resisting property of medicine generation of chemical pesticide, preserve the ecological environment, meet the requirement of environment protective plant protecting and Ecological Civilization Construction.

Description

A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants and its application
Technical field
The present invention relates to technical field of pesticide, more particularly to a kind of wide-spectrum bactericide for preventing and treating viral diseases of plants and its should With.
Background technology
Cytosintetidemycin belongs to cytosine nucleoside novel antiviral farm antibiotics, is by S. ahygroscopicus (Streptomyces achygruscop icus) is produced, and has preventive and therapeutic action to the viroses of plant.Product low toxicity, Low-residual, virus structure, reduction virion concentration, the ability for improving plant resistance virus can be destroyed as pesticides application, from And the effect for preventing and treating virosis is reached, it also can induce plant and produce resistance protein, improve the immunity of plant.
Amino-oligosaccharide is agricultural chitosan oligosaccharide, by exciting the immune response of plant itself, plant is obtained systematicness anti- Property so as to playing degeneration-resistant, disease-resistant and production-increasing function.Amino-oligosaccharide product low toxicity, is absorbed after being easily degraded by microorganisms by environment, Noresidue;Resistance of wide spectrum is had by the plant of its induction, the duration is long, and is not likely to produce the resistance to the action of a drug;Also have and improve crop Yield, improves the effect of crop quality.
Chinese invention patent CN1930991A discloses a kind of composite bactericide containing bacillus licheniformis, and the bactericide exists On the basis of bacillus licheniformis, other bactericide are added, so as to expand the use unit of composite bactericide, although the invention A variety of biological species and chemical series bactericidal agent (including cytosintetidemycin and amino-oligosaccharide) are listed, but the invention is not to each Plant the synergy of bactericide each other to be studied, in order to further improve the bactericidal effect of wide-spectrum bactericide, it is necessary to The wide-spectrum bactericide for preventing and treating viral diseases of plants further is studied.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of wide-spectrum bactericide for preventing and treating viral diseases of plants.
Technical scheme is as follows:
A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants, including following active ingredient:Cytosintetidemycin and amino-oligosacchride Element.
It is preferred that, in described active ingredient, cytosintetidemycin:Amino-oligosaccharide=(0.05~1.0):(0.05~1.0).
It is preferred that, in described wide-spectrum bactericide, the total content of active ingredient cytosintetidemycin and amino-oligosaccharide is sterilization The 2.5%~25% of agent.
It is preferred that, in described wide-spectrum bactericide, also comprising sodium benzoate, ethyl acetate, diglycerol polypropylene glycol ester and Water.
It is preferred that, in described wide-spectrum bactericide, the content point of sodium benzoate, ethyl acetate and diglycerol polypropylene glycol ester Wei 0.5%~2%, 0.05%~0.3% and 5%~10%.
The present invention is advantageous in that:
The present invention prepares two kinds of pharmaceutical agent combinations of cytosintetidemycin and amino-oligosaccharide, with advantages below:1. cytosintetidemycin There is induction, prevention and treatment multiple action, its synergistic effect is notable after being mixed by a certain percentage with amino-oligosaccharide.2. diseases prevention Spectrum is wide, has preventive and therapeutic effect to the multiple diseases of a variety of host plants, the especially preventing and treating to virosis has more remarkable effect.③ Induce plant to produce immunity, the defence capability of plant itself can be made full use of, that reduces chemical pesticide uses and resist the property of medicine The risk of generation, preserves the ecological environment, and meets the requirement of environment protective plant protecting and Ecological Civilization Construction.
Embodiment
The application principle of the present invention is explained in detail with specific embodiment below.
Embodiment 1
A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants, cytosintetidemycin and amino-oligosaccharide active ingredient account for 2.5%. Calculated by 100kg, include the raw material of following percentage by weight:30% cytosintetidemycin 5kg, 90% amino-oligosaccharide 1.11kg, benzene Sodium formate 1.2kg, ethyl acetate 0.2kg, diglycerol polypropylene glycol ester 8kg, water surplus.
Embodiment 2
A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants, cytosintetidemycin and amino-oligosaccharide active ingredient are accounted for 11.4%.Calculated by 100kg, include the raw material of following percentage by weight:30% cytosintetidemycin 2kg, 90% amino-oligosaccharide 12kg, sodium benzoate 0.5kg, ethyl acetate 0.2kg, diglycerol polypropylene glycol ester 5kg, water surplus.
Embodiment 3
A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants, cytosintetidemycin and amino-oligosaccharide active ingredient are accounted for 5.22%.Calculated by 100kg, include the raw material of following percentage by weight:30% cytosintetidemycin 15kg, 90% amino-oligosaccharide 0.8kg, sodium benzoate 2kg, ethyl acetate 0.05kg, diglycerol polypropylene glycol ester 10kg, water surplus.
Comparative example 1
Diglycerol polypropylene glycol ester in embodiment 1 is removed, remaining proportioning is constant.
Application example 1
By taking the wide-spectrum bactericide of embodiment 1 as an example, research cytosintetidemycin and amino-oligosaccharide compounding are to tobacco mosaic virus (TMV) Inhibitory action.
1. material to be tested
Medicament:2.5% cytosintetidemycin and amino-oligosaccharide are compound aqueous, cytosintetidemycin, amino-oligosaccharide.
Pathogen:Tobacco mosaic virus (TMV) (TMV)
Host plant:Nicotiana glutinosa
2. experimental method
Using half leaf withered spot method.Zuo Banye smears 3 kinds of test medicines such as cytosintetidemycin and amino-oligosaccharide mix preparation, right Half leaf smears phosphate buffer, and TMV viruses are inoculated with after 12 hours.Withered spot number is investigated in inoculation after 5 days, calculate withered spot inhibiting rate, Formula:
Withered spot inhibiting rate (%)=[(control withered spot number-processing withered spot number)/control withered spot number] × 100%.
3. measurement result
Table 1 is the measurement result of cytosintetidemycin and amino-oligosaccharide compounding to the inhibitory action of tobacco mosaic virus (TMV).
4. conclusion
2.5% cytosintetidemycin and amino-oligosacchride promotor composition have obvious inhibitory action to TMV, better than its single dose;No In the case of adding diglycerol polypropylene glycol ester, the preventive effect to tobacco mosaic virus (TMV) has a certain impact.
Application example 2
This test arrangement, in Fuxin City Fu Meng counties of Liaoning Province, is on probationly Plain plot, soil is leaching dynamics.Cultivation pipe Reason is carried out according to the unified production specification in experimental plot.
1. material to be tested
Medicament:5.22% cytosintetidemycin and amino-oligosacchride promotor composition, cytosintetidemycin, amino-oligosaccharide.
Pathogen:Cucumber mosaic virus (CMV)
Host plant:Tomato
2. experimental method
Disease index is investigated, experiment sets zone, and four repetitions, plot area is 35 square metres, there are about 100 plants.Yu Fa Sprayed (before early stage ten days) for the first time when sick, medicament is sprayed again once after seven days, spray dose for 45L/ mus.
3. investigation method
Whole plant in the every cell of investigation, investigation morbidity grade scale is carried out by disease survey grade scale.
Tomato virus disease classification investigation standard (investigation is classified in units of strain):
0 grade:Without any symptom;
1 grade:Floral leaf is presented in the bright arteries and veins of lobus cardiacus or 1~2 leaf;
3 grades:Middle and upper part blade floral leaf;
5 grades:In addition to most blade floral leaves, a small number of leaf malformations;
7 grades:Most blade weight floral leaves, deformity, shrinkage or plant are downgraded;
9 grades:Most blade weight floral leaves, deformity, plant are substantially downgraded, or even withered death.
Disease index calculation formula:
4. result of the test
Table 2 is the measurement result of cytosintetidemycin and amino-oligosaccharide compounding to the inhibitory action of cucumber mosaic virus.
5. conclusion
5.22% cytosintetidemycin and amino-oligosacchride promotor composition have notable preventive effect to tomato virus disease, are superior to phonetic peptide mould The single dose of element and amino-oligosaccharide;In the case of being added without diglycerol polypropylene glycol ester, the preventive effect to tomato virus disease has necessarily Influence.
This medicament can be used for the multiple diseases as caused by virus on the crops such as preventing and treating tobacco, vegetables, corn, watermelon.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto, Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.

Claims (8)

1. a kind of wide-spectrum bactericide for preventing and treating viral diseases of plants, it is characterised in that including following active ingredient:Cytosintetidemycin and Amino-oligosaccharide.
2. the wide-spectrum bactericide of viral diseases of plants is prevented and treated as claimed in claim 1, it is characterised in that described active ingredient In, cytosintetidemycin:Amino-oligosaccharide=(0.05~1.0):(0.05~1.0).
3. the wide-spectrum bactericide of viral diseases of plants is prevented and treated as claimed in claim 1, it is characterised in that described broad-spectrum sterilization In agent, the total content of active ingredient cytosintetidemycin and amino-oligosaccharide is the 2.5%~25% of bactericide.
4. the wide-spectrum bactericide of the preventing and treating viral diseases of plants as described in claim 1-3, it is characterised in that described wide spectrum is killed In microbial inoculum, also comprising sodium benzoate, ethyl acetate, diglycerol polypropylene glycol ester and water.
5. the wide-spectrum bactericide of viral diseases of plants is prevented and treated as claimed in claim 4, it is characterised in that described broad-spectrum sterilization In agent, the content of sodium benzoate, ethyl acetate and diglycerol polypropylene glycol ester be respectively 0.5%~2%, 0.05%~0.3% With 5%~10%.
6. preventing and treating the wide-spectrum bactericide of viral diseases of plants as claimed in claim 4, it is characterised in that calculate by 100kg, wrap Include the raw material of following percentage by weight:30% cytosintetidemycin 5kg, 90% amino-oligosaccharide 1.11kg, sodium benzoate 1.2kg, second Acetoacetic ester 0.2kg, diglycerol polypropylene glycol ester 8kg, water surplus.
7. it is a kind of prevent and treat viral diseases of plants wide-spectrum bactericide, it is characterised in that the wide-spectrum bactericide be applied to preventing and treating tobacco, The multiple diseases as caused by virus on veterinary antibiotics crop.
8. a kind of wide-spectrum bactericide for preventing and treating viral diseases of plants, it is characterised in that the wide-spectrum bactericide is applied to preventing and treating tobacco Mosaic virus or cucumber mosaic virus.
CN201710425346.XA 2017-06-08 2017-06-08 A kind of wide-spectrum bactericide for preventing and treating viral diseases of plants and its application Pending CN107212002A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111320597A (en) * 2020-03-26 2020-06-23 吴元华 Anti-plant virus pyriminomycin and preparation process and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103430946A (en) * 2013-08-23 2013-12-11 北京明德立达农业科技有限公司 Plant-virus resisting composition and plant-virus resisting medicament as well as application of plant-virus resisting medicament

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103430946A (en) * 2013-08-23 2013-12-11 北京明德立达农业科技有限公司 Plant-virus resisting composition and plant-virus resisting medicament as well as application of plant-virus resisting medicament

Non-Patent Citations (2)

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Title
刘刚: "三氮唑核苷产品将淘汰", 《农药市场信息》 *
朱春玉 等: "嘧肽霉素对烟草花叶病毒抑制作用研究", 《植物保护》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111320597A (en) * 2020-03-26 2020-06-23 吴元华 Anti-plant virus pyriminomycin and preparation process and application thereof
CN111320597B (en) * 2020-03-26 2022-03-11 吴元华 Anti-plant virus pyriminomycin and preparation process and application thereof

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Application publication date: 20170929

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