CN107200641A - A kind of lentinus edodes strain stick and preparation method thereof - Google Patents
A kind of lentinus edodes strain stick and preparation method thereof Download PDFInfo
- Publication number
- CN107200641A CN107200641A CN201710513492.8A CN201710513492A CN107200641A CN 107200641 A CN107200641 A CN 107200641A CN 201710513492 A CN201710513492 A CN 201710513492A CN 107200641 A CN107200641 A CN 107200641A
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- lentinus edodes
- edodes strain
- strain stick
- carbendazim
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
Abstract
The invention provides a kind of lentinus edodes strain stick and preparation method thereof, the lentinus edodes strain stick is using the raw material of konjaku bar, corncob, sunflower pole, rice bran, carbendazim, gypsum and ammonium sulfate mixing as lentinus edodes strain stick, it ensure that the nutrition needed for mushroom cultivating process, improve the yield and quality of mushroom, and raw material sources are abundant, cost is low, obtained lentinus edodes strain stick is reusable, it is especially suitable for the cultivation of mushroom, and this lentinus edodes strain stick preparation method is simple to operate, the operating time is short, it is adapted to large-scale production, with important economic benefit.
Description
Technical field
The present invention relates to mushroom cultivation field, and in particular to a kind of lentinus edodes strain stick and preparation method thereof.
Technical background
Mushroom [Lentinus edodes (Berk.) Sing] alias mushroom, dried mushroom, trade name flower mushroom.It is under the jurisdiction of fungi
Door Basidiomycetes Agaricales Tricholomataceae Lentinus, it is nutritious with fragrance, delicious characteristic, it is that whole world famous dish is conventional
One of major ingredient and dispensing, are liked by numerous users.Mushroom is described as " edible mushroom queen ", is domestic and international foremost food
With one of bacterium.Contain the mineral matters such as protein, fat, carbohydrate, crude fibre, calcium iron phosphorus and the life of substantial amounts of dimension in mushroom
Plain composition, strengthens resistance of human body, contributes to children's bone and tooth development, while preventing the elderly to suffer from osteoporosis.It is fragrant
Mushroom is sweet mild-natured, has effects that QI invigorating qi-restoratives, strengthening the spleen and stomach, and it can also reduce the cholesterol level in blood, follow blood
Ring remains normal, improves heart and brain microcirculation blood supply.
Using wood chip as primary raw material, but in today of scarcity of resources more than cultivating champignon, wood chip often causes ring
Border is destroyed, and it is higher using wood chip to cultivate mushroom cost, therefore, and the material of wood chip can be substituted and given birth to as primary raw material by searching out
Production mushroom simultaneously ensure that mushroom quality and yield have important economic benefit.
The content of the invention
The present invention is solves above-mentioned deficiency of the prior art, and there is provided one for progress inventive improvements in existing technology
Lentinus edodes strain stick and preparation method thereof is planted, the lentinus edodes strain stick uses konjaku bar, corncob, sunflower pole, rice bran, carbendazim, gypsum
The raw material as lentinus edodes strain stick is mixed with ammonium sulfate, the nutrition needed for mushroom cultivating process is ensure that, the production of mushroom is improved
Amount and quality, and raw material sources are abundant, cost is low, and obtained lentinus edodes strain stick is reusable, non-with important economic benefit
The often cultivation of suitable mushroom.
Specifically, the invention provides a kind of lentinus edodes strain stick, by weight, by 30-40 parts of konjaku bar, corncob 12-
19 parts, 30-40 parts of sunflower pole, 25-33 parts of rice bran, 0.1-0.3 parts of carbendazim, 0.8-1.5 parts of gypsum, 2-8 parts of ammonium sulfate system
Into.
It is preferred that, lentinus edodes strain stick, by weight, by 33-38 parts of konjaku bar, 13-18 parts of corncob, sunflower pole 34-
37 parts, 28-31 parts of rice bran, 0.1-0.3 parts of carbendazim, 1.0-1.4 parts of gypsum, 4-6 parts of ammonium sulfate is made.
It is furthermore preferred that lentinus edodes strain stick, by weight, by 35 parts of konjaku bar, 16 parts of corncob, sunflower pole 36 part, meter
30 parts of chaff, 0.2 part of carbendazim, 1.2 parts of gypsum, 5 parts of ammonium sulfate are made.
Present invention also offers a kind of preparation method of lentinus edodes strain stick, comprise the following steps：
(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, adds after carbendazim and gypsum, stir, made
Into bacterium rod；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5-6 hours,
In the indoor cooling by sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 1-2min, punching after inoculation, is used
Nanometer oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
It is preferred that, the preparation method of lentinus edodes strain stick comprises the following steps：
(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, added after carbendazim, ammonium sulfate and gypsum, stirred
Uniformly, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5 hours,
By the indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 2min, punching, after inoculation, with receiving
Rice oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
Further, described thimerosal is 0.1% liquor potassic permanganate.
Due to using above-mentioned technical proposal, the present invention has the advantages that：
The present invention is mixed according to a certain percentage using konjaku bar, corncob, sunflower pole, instead of wood chip, konjaku bar, jade
Meter Xin, sunflower pole belong to discarded object, and it is reusable to be fabricated to lentinus edodes strain stick, has saved resource, has turned waste into wealth, has greatly reduced
Production cost；Konjaku bar, corncob, sunflower pole, rice bran, carbendazim, gypsum and ammonium sulfate mixing are used as lentinus edodes strain stick
Raw material, can guarantee that mushroom cultivates required nutrition, improves the yield and quality of mushroom；In addition, lentinus edodes strain stick preparation side of the present invention
In method using can autoclave bags pack and autoclaving, using 25% alcohol solution dipping strain, 0.1% Gao Meng is used after inoculation
Sour potassium solution spray disinfectant, by multi-step sterilization, sterilization program in strict control lentinus edodes strain stick production process has
Effect prevent living contaminants, it is ensured that grand style, annesl, fruiting, product quality and the yield of lentinus edodes strain stick, can autoclave bags can
Reuse, save production cost, and this lentinus edodes strain stick preparation method is simple to operate, the operating time is short, is adapted to large-scale production,
It is economical.
Beneficial effect of the present invention is investigated：
1st, influence of the lentinus edodes strain stick raw material of the present invention to cultivating champignon
1.1 test strain
Xianggu mushroom strain L607.
1.2 lentinus edodes strain sticks are prepared and are grouped
Using preparation method of the present invention, lentinus edodes strain stick is made according to different material formula respectively in each group.
Test group 1：By konjaku bar 28kg, corncob 11kg, sunflower pole 28kg, rice bran 24kg, carbendazim 0.08kg, stone
Lentinus edodes strain stick made from cream 0.7kg, ammonium sulfate 1kg；(low component)
Test group 2：By konjaku bar 30kg, corncob 12kg, sunflower pole 30kg, rice bran 25kg, carbendazim 0.1kg, stone
Lentinus edodes strain stick made from cream 0.8kg, ammonium sulfate 2kg；
Test group 3：By konjaku bar 33kg, corncob 13kg, sunflower pole 34kg, rice bran 28kg, carbendazim 0.1kg, stone
Lentinus edodes strain stick made from cream 1.0kg, ammonium sulfate 4kg；
Test group 4：By konjaku bar 35kg, corncob 16kg, sunflower pole 36kg, rice bran 30kg, carbendazim 0.2kg, stone
Lentinus edodes strain stick made from cream 1.2kg, ammonium sulfate 5kg；
Test group 5：By konjaku bar 38kg, corncob 18kg, sunflower pole 37kg, rice bran 31kg, carbendazim 0.3kg, stone
Lentinus edodes strain stick made from cream 1.4kg, ammonium sulfate 6kg；
Test group 6：By konjaku bar 40kg, corncob 19kg, sunflower pole 40kg, rice bran 33kg, carbendazim 0.3kg, stone
Lentinus edodes strain stick made from cream 1.5kg, ammonium sulfate 8kg；
Test group 7：By konjaku bar 42kg, corncob 20kg, sunflower pole 42kg, rice bran 26kg, carbendazim 0.4kg, stone
Lentinus edodes strain stick made from cream 1.6kg, ammonium sulfate 1kg；(high component)
Test group 8：Poplar bits 70kg, wheat bran 20kg, gypsum 1%, sugar 1kg, carbendazim 0.1kg；(traditional lentinus edodes strain stick is former
Material)
Test group 9：According to lentinus edodes strain stick made from CN201510298578.4；
Using preparation method of the present invention, lentinus edodes strain stick is made according to different material formula respectively in test group 1-8, then
Each test group mushroom growth situation is observed, is recorded.
Biological indicator：
Yield：In whole cultivating champignon season, each is formulated the average value (g) of each bacterium rod output mushroom gross weight
Gross dry weight × 100% of total fresh weight/cultivation matrix of the mushroom of biological efficiency %=outputs
Psychrometric ratio：In the mushroom fruiting body obtained from each formula harvesting, if each picking dried thin mushroom, it is ensured that gross weight is more than
200g, G is recorded after being weighed with assay balance.In electric drying oven with forced convection, 80 DEG C are baked and banked up with earth 8 hours to constant weight, use assay balance
Weigh, record g, then single psychrometric ratio calculation formula：R=(g/G) × 100%.
1.3 result of the test
| Yield g/ bacterium rods | Biological efficiency % | Psychrometric ratio % | |
| Test group 1 | 780.9 | 64.7 | 12.4 |
| Test group 2 | 895.3 | 69.2 | 13.7 |
| Test group 3 | 1013.5 | 72.5 | 14.8 |
| Test group 4 | 1145.6 | 75.1 | 15.6 |
| Test group 5 | 1107.8 | 74.8 | 15.3 |
| Test group 6 | 1046.2 | 74.2 | 15.0 |
| Test group 7 | 927.5 | 73.3 | 14.3 |
| Test group 8 | 690.4 | 61.2 | 11.3 |
| Test group 9 | 712.8 | 62.9 | 11.6 |
It can be seen that from upper table, test group 2-6 yield, biological efficiency and psychrometric ratio are above (the tradition perfume of test group 8
Mushroom rod raw material), test group 9, test group 1 (low component), show test group 2-6 more suitable for cultivating champignon, mushroom quality is more
Excellent, test group 7 is high component group, and yield, biological efficiency and psychrometric ratio slightly have reduction, therefore give up；The mushroom of test group 4 is produced
Amount, biological efficiency and psychrometric ratio highest, therefore, lentinus edodes strain stick preferred ingredient proportioning of the present invention is 30-40 parts of konjaku bar, corn
12-19 parts of core, 30-40 parts of sunflower pole, 25-33 parts of rice bran, 0.1-0.3 parts of carbendazim, 0.8-1.5 parts of gypsum, ammonium sulfate 2-8
Part, optimal component proportioning is：35 parts of konjaku bar, 16 parts of corncob, 36 parts of sunflower pole, 30 parts of rice bran, 0.2 part of carbendazim, stone
1.2 parts of cream, 5 parts of ammonium sulfate.
2nd, influence of the lentinus edodes strain stick preparation method of the present invention to cultivating champignon
Test method and packet：
Raw material：Konjaku bar 35kg, corncob 16kg, sunflower pole 36kg, rice bran 30kg, carbendazim 0.2kg, gypsum
1.2kg, ammonium sulfate 5kg
Test group 1：Lentinus edodes strain stick, which is made, according to preparation method of the present invention is used for cultivating champignon；
Test group 2：Lentinus edodes strain stick, which is conventionally made, is used for cultivating champignon：By konjaku bar, corncob, sunflower
Bar, rice bran are crushed, and are added after carbendazim, ammonium sulfate and gypsum, are stirred, bacterium rod is made；Filled using low-pressure polyethylene film
Bag, charging before polybag one end is shut, reach definitely it is air tight be defined, build soil steamer：From 1.5 meters of big iron of diameter
Pot, it is 2 × 2 × 2.5 meters to be built into length × width × height, and volume is 8~10 cubic metres of native steamer, external application height cement in every pot wall
Troweling, the curtain of 1.7 meters of diameter is made of flitch or reinforcing bar, is placed on pot face, gunnysack is spread above, to prevent puncturing pocket.Use again
Thickness is made a diameter of 2.1 meters of pot cover for 1.5 millimeters of galvanized sheet, steaming stock pot build up it is rear standby, toward pot in fill up enough water,
20 centimeters of water surface distance curtain, then the pocket consistency from top to bottom for tying mouth, puts on the curtain in steamer in batches in pot, on fire
Heating sterilizing, temperature reaches 100 DEG C in 4 hours, keeps this temperature 10 hours, then a vexed night, is robbed when material temperature is reduced to 70 DEG C
Temperature is taken the dish out of the pot, and cooling chamber is moved into rapidly, after being inoculated after temperature drop in pocket；The instrument of discharging is put into room using first 4 days
Interior sealing door and window, reaches that indoor sealing is air tight, room is fumigated after each room is lighted with sulphur, when pocket temperature drops to 30
DEG C when start to plant, light alcolhol burner, strain punching takes strain, mixes a bag tying point strain, seals；Indoor half-light is kept, is sent out
Ferment；
Biological indicator：
Yield：In whole cultivating champignon season, each is formulated the average value (g) of each bacterium rod output mushroom gross weight
Gross dry weight × 100% of total fresh weight/cultivation matrix of the mushroom of biological efficiency %=outputs
Psychrometric ratio：In the mushroom fruiting body obtained from each formula harvesting, if each picking dried thin mushroom, it is ensured that gross weight is more than
200g, G is recorded after being weighed with assay balance.In electric drying oven with forced convection, 80 DEG C are baked and banked up with earth 8 hours to constant weight, use assay balance
Weigh, record g, then single psychrometric ratio calculation formula：R=(g/G) × 100%.
Result of the test
| Yield g/ bacterium rods | Biological efficiency % | Psychrometric ratio % | |
| Test group 1 | 1137.2 | 74.6 | 15.6 |
| Test group 2 | 992.3 | 72.6 | 14.4 |
It can be seen that from upper table, lentinus edodes strain stick prepared using preparation method of the present invention, because sterilisation step of the present invention is strict,
And employ can autoclave bags pack and autoclaving, 25% alcohol solution dipping strain uses 0.1% potassium permanganate after inoculation
Spray solution is sterilized, and is compared with conventional method, can more be improved the yield of mushroom, it is ensured that the quality of mushroom.
Embodiment
Embodiment 1：The preparation of lentinus edodes strain stick
Formula：Konjaku bar 30kg, corncob 12kg, sunflower pole 30kg, rice bran 25kg, carbendazim 0.1kg, gypsum
0.8kg, ammonium sulfate 2kg
Preparation method：(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, adds carbendazim, ammonium sulfate and gypsum
Afterwards, stir, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5 hours,
By the indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 2min, punching, after inoculation, with receiving
Rice oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
Embodiment 2：The preparation of lentinus edodes strain stick
Formula：Konjaku bar 33kg, corncob 13kg, sunflower pole 34kg, rice bran 28kg, carbendazim 0.1kg, gypsum
1.0kg, ammonium sulfate 4kg
Preparation method：(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, adds carbendazim, ammonium sulfate and gypsum
Afterwards, stir, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5 hours,
By the indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 2min, punching, after inoculation, with receiving
Rice oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
Embodiment 3：The preparation of lentinus edodes strain stick
Formula：Konjaku bar 35kg, corncob 16kg, sunflower pole 36kg, rice bran 30kg, carbendazim 0.2kg, gypsum
1.2kg, ammonium sulfate 5kg
Preparation method：(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, adds carbendazim, ammonium sulfate and gypsum
Afterwards, stir, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5 hours,
By the indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 2min, punching, after inoculation, with receiving
Rice oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
Embodiment 4：The preparation of lentinus edodes strain stick
Formula：Konjaku bar 38kg, corncob 18kg, sunflower pole 37kg, rice bran 31kg, carbendazim 0.3kg, gypsum
1.4kg, ammonium sulfate 6kg
Preparation method：(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, adds carbendazim, ammonium sulfate and gypsum
Afterwards, stir, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5 hours,
By the indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 2min, punching, after inoculation, with receiving
Rice oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
Embodiment 5：The preparation of lentinus edodes strain stick
Formula：Konjaku bar 40kg, corncob 19kg, sunflower pole 40kg, rice bran 33kg, carbendazim 0.3kg, gypsum
1.5kg, ammonium sulfate 8kg
Preparation method：(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, adds carbendazim, ammonium sulfate and gypsum
Afterwards, stir, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5 hours,
By the indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 2min, punching, after inoculation, with receiving
Rice oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
Claims (6)
1. a kind of lentinus edodes strain stick, by weight, it is characterised in that by 30-40 parts of konjaku bar, 12-19 parts of corncob, sunflower
30-40 parts of bar, 25-33 parts of rice bran, 0.1-0.3 parts of carbendazim, 0.8-1.5 parts of gypsum, 2-8 parts of ammonium sulfate are made.
2. lentinus edodes strain stick according to claim 1, by weight, it is characterised in that by 33-38 parts of konjaku bar, corn
13-18 parts of core, 34-37 parts of sunflower pole, 28-31 parts of rice bran, 0.1-0.3 parts of carbendazim, 1.0-1.4 parts of gypsum, ammonium sulfate 4-6
Part is made.
3. lentinus edodes strain stick according to claim 1, by weight, it is characterised in that by 35 parts of konjaku bar, corncob 16
Part, 36 parts of sunflower pole, 30 parts of rice bran, 0.2 part of carbendazim, 1.2 parts of gypsum, 5 parts of ammonium sulfate are made.
4. a kind of preparation method of lentinus edodes strain stick, it is characterised in that comprise the following steps：
(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, added after carbendazim, gypsum and ammonium sulfate, stirring is equal
It is even, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5-6 hour, passing through
Cross the indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 1-2min, punching, after inoculation, with nanometer
Oxygen supply film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
5. the preparation method of lentinus edodes strain stick according to claim 4, it is characterised in that comprise the following steps：
(1) konjaku bar, corncob, sunflower pole, rice bran are crushed, added after carbendazim, ammonium sulfate and gypsum, stirring is equal
It is even, bacterium rod is made；
(2) using can autoclave bags pack, be placed in closed steamer, 0.1MPa, 121 DEG C of autoclavings 5 hours, passing through
The indoor cooling of sterilization；
(3) select that mycelia is healthy and strong, free of contamination strain, with 25% alcohol solution dipping 2min, punching after inoculation, is supplied with nanometer
Oxygen film is sealed, then in surface spray disinfectant；
(4) selection ventilation, the environment fermentation dried.
6. the preparation method of the lentinus edodes strain stick according to claim 4 or 5, it is characterised in that described thimerosal is 0.1%
Liquor potassic permanganate.
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| CN201710513492.8A CN107200641A (en) | 2017-06-29 | 2017-06-29 | A kind of lentinus edodes strain stick and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710513492.8A CN107200641A (en) | 2017-06-29 | 2017-06-29 | A kind of lentinus edodes strain stick and preparation method thereof |
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ID=59910437
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948355A (en) * | 2010-09-26 | 2011-01-19 | 黑龙江省农垦科学院 | Preparation method of culture medium of edible fungi |
| CN104350940A (en) * | 2014-10-17 | 2015-02-18 | 上海智城分析仪器制造有限公司 | Preparing method for edible fungi cultivation pressing blocks |
| CN105036924A (en) * | 2015-07-24 | 2015-11-11 | 中南民族大学 | Edible fungus culture medium with tea leaf waste as main raw materials |
-
2017
- 2017-06-29 CN CN201710513492.8A patent/CN107200641A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948355A (en) * | 2010-09-26 | 2011-01-19 | 黑龙江省农垦科学院 | Preparation method of culture medium of edible fungi |
| CN104350940A (en) * | 2014-10-17 | 2015-02-18 | 上海智城分析仪器制造有限公司 | Preparing method for edible fungi cultivation pressing blocks |
| CN105036924A (en) * | 2015-07-24 | 2015-11-11 | 中南民族大学 | Edible fungus culture medium with tea leaf waste as main raw materials |
Non-Patent Citations (1)
| Title |
|---|
| 王松良 等: "《食用菌栽培技术问答》", 31 March 1993 * |
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