CN1072005C

CN1072005C - Nutritive soy sauce preparing process of hydrolyzed animal and plant protein mixture

Info

Publication number: CN1072005C
Application number: CN98104693A
Authority: CN
Inventors: 姚恕; 郑连英; 陈昌定
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 1998-02-19
Filing date: 1998-02-19
Publication date: 2001-10-03
Anticipated expiration: 2018-02-19
Also published as: CN1191687A

Abstract

The present invention discloses a method for preparing nutritive soy sauce from hydrolyzed animal and plant protein mixtures. The method comprises: pulverizing 1kg of silkworm kidney in 2 to 7kg of water into pulpous liquid; heating, cooling and deoiling the pulpous liquid; successively, adding lipase and proteinase for enzymolysis; mixing with 1kg of defatted soy bean, successively adding 2 to 7kg of edible hydrochloric acid for hydrolysis, and adding NaCO3 to the press filtrate for neutralization; then, filtering to obtain neutralized liquid, adding active carbon to the neutralized liquid for decolorization and deodorization; filtering once more, adding edible salt, sugar, caramel, seasoning and potassium sorbate to the filtrate, uniformly stirring, successively filling and sterilizing. The present invention has the advantages of low cost and wide raw material source, the proportion of eight kinds of amino acids essential to human bodies approaches to the proportioning mode recommended by FAO, and the sanitary indexes of products are advanced.

Description

Hydrolyzed animal and plant protein mixture prepares the method for nutrient soya sauce

The present invention relates to the preparation method of soy sauce, relate in particular to the method that hydrolyzed animal and plant protein mixture prepares nutrient soya sauce.

Soy sauce on the market is most for making soy sauce now.Generally fermenting by soybean and by microorganism gets, and it has certain pure fragrance, contains its attitude nitrogen of ammonia, is greater than 0.4g/100ml by national standard.Solid content is greater than 12%, and salt (in NaCl) is greater than 15%.Be the traditional flavouring of China people, it only has a little delicate flavour, and bacteria containing amount higher (about 50000/ml).

The purpose of this invention is to provide the method that a kind of hydrolyzed animal and plant protein mixture prepares nutrient soya sauce.

The present invention takes following measures in order to achieve the above object: at first 1 kilogram of silkworm chrysalis and (2～7) kg water are broken into pulpous state liquid in the pulverizer of (500～1000) rev/min, heating makes pulpous state liquid be warmed up to (50～100) ℃, after keeping (20～80) minute, be cooled to (0～5) ℃ and keep (1～6) hour, remove oil slick, then add lipase and protease (10000～100000) units/kg silkworm chrysalis, enzymolysis (10～24) hour, then with after 1 kilogram of defatted soybean cooperates, add (2～7) kilogram (4～6) N edible hydrochloric acid, place hydrolyzer in (101～106) ℃ following hydrolysis (12～20) hour, in (90～95) ℃ following press filtration, in the filtrate dislocation and in the pot, add edible NaCO ₃The saturated aqueous solution neutralization reaction to PH be 4.5～5.5, refilter and remove slag to such an extent that neutralizer is introduced in the Decolouring pot, the activated carbon that adds (0.02～0.12) kilogram is in (90～95) ℃ decolouring down, deodorization (30～60) minute, filter once more filtrate is moved into the allotment pot, at last with salt, sugar, burnt sugar coloring, edible catalpa regulating material and potassium sorbate, after stirring, can, sterilization.

Advantage of the present invention: 1. silkworm chrysalis and defatted soybean are made raw material production nutrient soya sauce, production cost is low, it is wide that raw material sources adapt to, and with the reasonable cooperation of animals and plants mixed protein, the proportioning pattern that 8 kinds of essential amino acid proportionings of gained are more recommended near FAO (Food and Agriculture Organization of the United Nation); 2. utilize enzyme to have single catalytic reaction principle, the enzyme reaction method of employing modern biotechnology removes fat and the stink in the silkworm chrysalis, method advanced person, and the degreasing deodorization is complete; 3. this method makes the sanitary index advanced person of product owing to do not add blended soy sauce and convert and join, and bacteria containing amount is less than 5000/ml.

Elaborate below in conjunction with embodiment.

At first 1 kilogram of silkworm chrysalis and (3～5) kg water are broken into pulpous state liquid in the pulverizer of (500～1000) rev/min, heating makes pulpous state liquid be warmed up to (50～100) ℃, after keeping (30～50) minute, be cooled to (0～4) ℃ and keep (1～4) hour, remove oil slick, then add lipase and protease (10000～100000) units/kg silkworm chrysalis, enzymolysis (10～16) hour, then with after 1 kilogram of defatted soybean cooperates, add (2～7) kilogram (4～6) N edible hydrochloric acid, place hydrolyzer in (101～106) ℃ following hydrolysis (12～16) hour, in (90～95) ℃ following press filtration, in the filtrate dislocation and in the pot, add edible NaCO ₃The saturated aqueous solution neutralization reaction to PH be 4.5～5.5, refilter and remove slag to such an extent that neutralizer is introduced in the Decolouring pot, the activated carbon that adds (0.02～0.12) kilogram is in (90～95) ℃ decolouring down, deodorization (30～40) minute, filter once more filtrate is moved into the allotment pot, at last with salt, sugar, burnt sugar coloring, edible catalpa regulating material and potassium sorbate, after stirring, can, sterilization.

Embodiment:

At first 1 kilogram of silkworm chrysalis and 3 kg water are broken into pulpous state liquid in 1000 rev/mins pulverizer, heating makes pulpous state liquid be warmed up to 50 ℃, keeps after 30 minutes, being cooled to 3 ℃ kept 3 hours, remove oil slick, then add lipase and protease 10000 units/kg silkworm chrysalises, enzymolysis 12 hours, then with after 1 kilogram of defatted soybean cooperates, add 6 kilograms of 6N edible hydrochloric acids, place hydrolyzer 105 ℃ of following hydrolysis 13 hours, 95 ℃ of following press filtrations, in the filtrate dislocation and in the pot, add edible NaCO ₃The saturated aqueous solution neutralization reaction to PH be 5, refilter and remove slag to such an extent that neutralizer is introduced in the Decolouring pot, the activated carbon that adds 0.10 kilogram is in 90 ℃ of decolourings down, deodorization 30 minutes, filter once more filtrate is moved into the allotment pot, at last with salt, sugar, burnt sugar coloring, edible catalpa regulating material and potassium sorbate, after stirring, can, sterilization.

Claims

1. a hydrolyzed animal and plant protein mixture prepares the method for nutrient soya sauce, it is characterized in that: at first 1 kilogram of silkworm chrysalis and (2～7) kg water are broken into pulpous state liquid in the pulverizer of (500～1000) rev/min, heating makes pulpous state liquid be warmed up to (50～100) ℃, after keeping (20～80) minute, be cooled to (0～5) ℃ and keep (1～6) hour, remove oil slick, then add lipase and protease (10000～100000) units/kg silkworm chrysalis, enzymolysis (10～24) hour, then with after 1 kilogram of defatted soybean cooperates, add (2～7) kilogram (4～6) N edible hydrochloric acid, place hydrolyzer in (101～106) ℃ following hydrolysis (12～20) hour, in (90～95) ℃ following press filtration, in the filtrate dislocation and in the pot, add edible NaCO ₃The saturated aqueous solution neutralization reaction to PH be 4.5～5.5, refilter and remove slag to such an extent that neutralizer is introduced in the Decolouring pot, the activated carbon that adds (0.021～0.12) kilogram is in (90～95) ℃ decolouring down, deodorization (30～60) minute, filter once more filtrate is moved into the allotment pot, at last with salt, sugar, burnt sugar coloring, edible catalpa regulating material and potassium sorbate, after stirring, can, sterilization.