CN1035425A

CN1035425A - The manufacture method of blue green alga-spiral alga drink

Info

Publication number: CN1035425A
Application number: CN89100036A
Authority: CN
Inventors: 冯春发; 彭素萍
Original assignee: Jiangxi Normal University
Current assignee: Jiangxi Normal University
Priority date: 1988-12-29
Filing date: 1988-12-29
Publication date: 1989-09-13

Abstract

A kind of natural colouring matter that is had that utilizes itself, make the method for the transparent protein amino acid beverage of blueness, it is to be primary raw material with blue-green algae-spirulina, through levigate, leaching, at the control solution temperature, the pH value, under the condition in reaction time, adopt enzymatic hydrolysis, obtain the protein amino acid solution, this solution assistant is with other acids, sweetener, an amount of essence makes the beverage that contains higher white matter and contain 18 seed amino acids, especially contains the necessary 8 kinds amino acid of human body in this beverage, vitamin C and carrotene, beverage does not use anticorrisive agent, and it is a kind of nutritious, the health drink of sweet and sour taste and have the low characteristics of production cost.

Description

The manufacture method of blue green alga-spiral alga drink

The invention belongs to beverage manufacturing method, particularly utilize blue-green algae to have the transparent protein amino acid beverage of self natural blue for the raw material manufacturing.

Blue green alga-spiral alga (Spinuling platensis) is a kind of plant protein resource that has developed on the international market since nineteen seventies.It contains rich in protein, natural blue-green element, glucan, mineral matter and beta carotene.For the development and use of blue-green algae, following several approach is arranged roughly: 1, keep the processing method of original composition and local flavor, it is that assistant is edible together with other food in clean blue-green algae; 2, in blue-green algae, mix the method for other additive improved local flavor, as making cake, biscuit, ice river in Henan Province beautiful jade or add in other food blue algae powder and cheese, milk powder, essence being mixed, to improve its quality as additive.And directly from blue-green algae, utilize self blueness, and need not add pigment, make transparently, rather than the method for the protein amino acid beverage of suspension is not appeared in the newspapers as yet.

This is protein and the natural blue pigment that utilizes blue green alga-spiral alga itself to give with bright purpose, and a kind of method of making the transparent protein amino acid beverage of blueness is provided.This method is to utilize blue-green algae dry powder, to make catalyst through selected enzyme, under the condition of certain acidity, temperature and activator, with the cyanophycin hydrolysis, produces a kind of being easy to and digests and assimilates, transparent and nutritious protein amino acid beverage.

The present invention utilizes blue green alga-spiral alga to be raw material, obtains the beverage of certain polypeptide, amino acid and soluble protein through enzymatic hydrolysis.With regard to blue-green algae, its protein content is up to more than 60%, and content beta-carotene reaches 1.90 gram/kilograms, contains the carbohydrate of the activity of earning a bare living in addition and regulates the plurality of inorganic salt and the trace element of vital movement.

Table 1 blue-green algae and the mainly comparison of animals and plants nutrition

The cell membrane of blue-green algae is that crude protein is formed, and lignocellulose-containing is few.Therefore be easy to digest and assimilate, its digestibility can reach 85%.Has multiple natural colouring matter in the blue-green algae, as chlorophyll, lutein, beta carotene, algocyan etc., in process, as long as the technical conditions of controlling well, these natural colouring matters can not destroy, and this just provides necessary material conditions for making blue-green algae natural colouring matter beverage.

The method that the present invention makes the blue-green algae beverage is, get clean, dry algae powder through sterilization carries out levigate, add a certain amount of water extraction 2～3 times, filter, get filtrate or without the solution and the algae mud that filter, drip an amount of acid, make pH value transfer to PH=4～9, in 30～62 ℃ of temperature ranges, with enzyme hydrolysis 1.5～6 hours, adjustment of acidity makes PH=2.8 in the solution～3.5 then, places 4～8 hours, isolate solid slag, supernatant is by the vacuum removal peculiar smell, and the hydrolysate assistant that obtains is like this prepared burden with other, can make to have the transparent of natural blue pigment and contain soluble protein and amino acid whose beverage.The inventor thinks that selecting suitable enzyme is important to hydrolytic process, and the present invention uses that trypsase, aspergillus oryzae, papain, enzyme are clear, bromelain and the protease and the starch that extract from hay bacillus all are effective.Simultaneously, the inventor notices that the pH value of solution is very big to the hydrolysis influence of enzyme, and it not only may cause inactivation, the substrate precipitation of enzyme, the color and luster of also strong left and right sides hydrolysate.When using certain enzyme hydrolysis, as PH＞8.5, then solution is colourless after the hydrolysis, and other enzymes can obtain more stable color and luster with this understanding, certainly, it also is a key factor that the concentration of substrate is determined, concentration of substrate is too high, yield descends, the cost height, when concentration was on the low side, hydrolysis rate was slow, reaction time is long, and yield is also low.At last, enzyme is in hydrolytic process, and it also is vital being subjected to some cationic activation, otherwise even other condition is selected well, the hydrolysis yield is also very low for a long time.The effect of blue-green algae hydrolysis is subjected to the control of six factors, that is, and and the kind of enzyme, concentration of substrate, pH value, reaction time, reaction temperature and activator.In general, enzyme dry algae powder=1: 200～400(is in weight hundred ratios) be that effectively the solution pH value is 4～9, temperature is controlled at 30～62 ℃, reacts 1.5～6 hours, uses Na ⁺, Ca ²⁺Or Mg ²⁺Make activator, can obtain effect preferably.Its product can concentrate or towards rare, be made into the beverage of various concentration, now lists in table 2 with a kind of amino acid composition of product.Its soluble protein content is 6 grams per liters.

Method provided by the present invention can obtain having the beverage of following character:

Color and luster: light blue clarity: limpid transparent nothing precipitation

Smell: free from extraneous odour

Amino acid content in the table 2 blue-green algae beverage

Mouthfeel: sweet and sour taste preservative free

Soluble protein 4～8 grams per liters

Total amino acid content 250～1000 mg/litre

Calcium 1150 mg/litre iron 1～3 mg/litre

Potassium 42～50 mg/litre magnesium 10～15 mg/litre

Vitamin C 60～200 mg/litre

Total acid content: 0.15～0.20% white sugar 8～10%

Other is Co ₂And water.

1), raw material nutrition is many present invention compares with the existing beverage technology of making, and has following distinguishing feature:, preparing high-quality beverage does not need or seldom needs to add other additives; 2), the wide material sources of enzyme, beverage need not add pigment, the intrinsic natural colouring matter of utilization itself is as colouring agent; 3), beverage does not add anticorrisive agent, the drinking safety height; 4), protein and amino acid content are higher and contain multiple natural mineral matter, be easy to digest and assimilate.

Embodiment

Example 1. is levigate blue green alga-spiral alga powder totally, uses flooding 2～3 times, gets supernatant in PH=5～8,50 ℃ of temperature, and enzyme: dry algae powder=1: 300 adds micro-Ca ²⁺, with trypsin hydrolysis 1.5～2.5 hours, filter, to regulate PH=3 and make enzyme deactivation, sterilization again after vacuum is taken off peculiar smell adds other additives and makes limpid transparently, has the protein amino acid beverage of natural blue pigment.

Example 2, with blue-green algae dry powder with flooding 1～2 time, supernatant is with algae mud, sterilization adds the Na of trace ⁺Or Ca ²⁺, in PH=7.5～8.5,45～62 ℃ of temperature were separated 2～3 hours enzyme: blue-green algae dry powder=1: 400 with papain or enzyme clear water.Filter, make filtrate inactivation under PH=3.5, vacuum is taken off peculiar smell, and sterilization mixes with citric acid, granulated sugar and vitamin C, is mixed with sweet and sour taste, the transparent protein beverage with natural blue.

Example 3, dry algae powder is become pasty state with water mill, with protease and the amylase that extracts from hay bacillus: algae is stuck with paste=1: 250 ratio, makes PH=6～8, and 50 ℃ of temperature add a small amount of Mg ²⁺Or Ca ²⁺, hydrolysis 3～4 hours, centrifugal filtration, filtrate is transferred PH=3.2, vacuum deodorization, sterilization, assistant has the transparent protein beverage of natural blue with other raw material preparations.

Example 4, dry algae powder 5 gram ground to form pasty state with low amounts of water after, add 20 times water extraction, sterilization makes phycocolloid mud PH=5～8, adds micro-Na ⁺With a certain amount of aspergillus oryzae extract, enzyme: dry algae powder=1: 200,45～52 ℃ of following hydrolysis of temperature 2～4 hours, centrifugation, filtrate are transferred after the PH=3, static 3～4 hours, sediment separate out, filtrate are through vacuum deodorization, after the sterilization, add 100 milligrams of vitamin Cs again, white granulated sugar 40 grams, a small amount of essence adds water to 500 milliliters, regulating total acid content with citric acid is 1.8 grams per liters, promptly makes blue transparent protein amino acid beverage.

Claims

1, a kind of blue-green algae--spirulina manufacturing protein that utilizes, the method of amino acid beverage, it be with blue-green algae levigate after, with flooding 2～3 times, with its supernatant or extract with algae mud, the acid-base value of regulator solution is PH=4～9, add a certain amount of enzyme again as catalyst, temperature is controlled at 30～62 ℃ of scopes, hydrolysis 1.5～6 hours, centrifugation, solution PH transfers to 2.8～3.5 and makes enzyme deactivation, then with other acids, sweetener, vitamin C and carbonated water are mixed with the natural blue transparent protein of sweet and sour taste, amino acid beverage, its Main Ingredients and Appearance is:

Content of soluble protein 4～8 grams per liters

Total amino acid content content 280～1000 mg/litre

Calcium: 1150 mg/litre iron, 1～3 mg/litre

Potassium: 42～50 mg/litre magnesium, 10～15 mg/litre

Vitamin C 60～200 mg/litre

Total acid content: 0.15～0.20% sugar 8～10%

Other is CO ₂And water.

2, according to the blue-green algae beverage manufacturing method of claim 1, it has used enzyme to be hydrolyzed as catalyst, it is characterized in that having used at least a kind of in the following enzyme to be hydrolyzed a) papain as catalyst; B) trypsase; C) aspergillus oryzae; D) bromelain; E) protease that from hay bacillus, extracts or amylase.

3, according to the blue-green algae beverage manufacturing method of claim 1 or 2, when it is characterized in that used enzyme is made catalyst, the weight ratio of enzyme and blue-green algae dry powder is: enzyme: dry algae powder=1: 200～400.

4, according to the blue-green algae beverage manufacturing method of claim 3, it is hydrolysis under acidity or weak basic condition, it is characterized in that:

A) pH value of solution should be controlled under the condition of PH=4～9 and carry out;

B) temperature of solution is controlled at 30～62 ℃, reacts 1.5～6 hours;

C) enzyme of Shi Yonging has adopted Na at least through activating ⁺, Ca ²⁺Or Mg ²⁺In one or more make activator.

5, according to the preparation method of the blue-green algae beverage of claim 4, the mixed solution that obtains behind enzymatic hydrolysis must carry out inactivation and handle, and it is characterized in that selecting the acidity condition of PH=2.8～3.5 to make enzyme deactivation in the solution.

6, according to the blue-green algae beverage manufacturing method of claim 1, it has selected vitamin C for use is acid, it is characterized in that ascorbic addition is 60～200 mg/litre.

7, according to the preparation method of the blue-green algae beverage of claim 1, it is characterized in that the blue color of beverage is the blue phycochrome of blue-green algae itself, need not to add other pigment.

8,, it is characterized in that not adding in the beverage anticorrisive agent according to the blue-green algae beverage manufacturing method of claim 1 or 6 or 7.