JP6275638B2 - Flavor improver - Google Patents

Flavor improver Download PDF

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JP6275638B2
JP6275638B2 JP2014513366A JP2014513366A JP6275638B2 JP 6275638 B2 JP6275638 B2 JP 6275638B2 JP 2014513366 A JP2014513366 A JP 2014513366A JP 2014513366 A JP2014513366 A JP 2014513366A JP 6275638 B2 JP6275638 B2 JP 6275638B2
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flavor
food
hydrolyzate
parts
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JPWO2013164982A1 (en
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田 陽 平 藤
田 陽 平 藤
上 典 明 井
上 典 明 井
田 隆 生 木
田 隆 生 木
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MC Food Specialties Inc
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/56Flavouring or bittering agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/20Products from fruits or vegetables; Preparation or treatment thereof by pickling, e.g. sauerkraut or pickles
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00Soups; Sauces; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/21Synthetic spices, flavouring agents or condiments containing amino acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/60Salad dressings; Mayonnaise; Ketchup
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/88Taste or flavour enhancing agents

Description

関連出願の参照Reference to related applications

本特許出願は、2012年5月1日に出願された日本国特許出願2012−104640号に基づく優先権の主張を伴うものであり、かかる先の特許出願における全開示内容は、引用することにより本明細書の一部とされる。   This patent application is accompanied by a priority claim based on Japanese Patent Application No. 2012-104640 filed on May 1, 2012, and the entire disclosure in such earlier patent application is incorporated by reference. It is made a part of this specification.

本発明は、飲食品の風味改良剤および風味改良方法に関する。   The present invention relates to a flavor improving agent and a flavor improving method for foods and drinks.

アミノ酸は、種々の食材に含まれ、食品の味に大きく寄与している。従来、鏡像異性体のないグリシン以外は、L−体のみが風味に関与していると考えられていた。例えば、非特許文献1には、大豆蛋白質の加水分解によりD−アミノ酸が生じることが記載されている。しかしながら、D−アミノ酸の風味への関与については何ら記載されていない。   Amino acids are contained in various foods and greatly contribute to the taste of food. Conventionally, it was thought that only the L-form was involved in the flavor, except for glycine without an enantiomer. For example, Non-Patent Document 1 describes that D-amino acid is generated by hydrolysis of soybean protein. However, there is no description about the involvement of D-amino acids in the flavor.

近年の研究から、D−アミノ酸のなかにも味に関与するものがあることが示され始めている。例えば、特許文献1には、D−アラニンを添加して旨みを増加させる日本酒の製造方法が記載されている。   Recent research has begun to show that some D-amino acids are also related to taste. For example, Patent Document 1 describes a method for producing sake that adds D-alanine to increase umami.

また、非特許文献2には、D−アスパラギン酸高含有の日本酒が報告されている。   Non-Patent Document 2 reports sake containing a high content of D-aspartic acid.

特開2012―005425公報JP 2012-005425 A

農芸化学会誌、第36巻、第6号、p.469〜473, 1962Agricultural Chemical Society Journal, Vol. 36, No. 6, p. 469-473, 1962 2012年農芸化学会要旨 講演番号2C20p132012 Agricultural Chemistry Society Abstract No. 2C20p13

本発明は、風味改良効果に優れた蛋白質加水分解物、または風味改良剤を提供することを目的とする。   An object of this invention is to provide the protein hydrolyzate excellent in the flavor improvement effect, or a flavor improving agent.

本発明によれば以下の発明が提供される。
(1)D−プロリンまたはその塩を含有する蛋白質加水分解物であって、
無機塩を除いた蛋白質加水分解物の固形分100質量部に対してD−プロリンを0.5質量部以上含有する蛋白質加水分解物。
(2)無機塩を除いた蛋白質加水分解物の固形分100質量部に対してD-アスパラギン酸を1質量部以上含有する、(1)に記載の蛋白質加水分解物。
(3)無機塩を除いた蛋白質加水分解物の固形分100質量部に対してD−グルタミン酸を0.7質量部以上含有する、(1)または(2)に記載の蛋白質加水分解物。
(4)蛋白質加水分解物が、蛋白質を水素イオン濃度5mol/L以上の溶液中で加水分解して得られる蛋白質である、(1)〜(3)のいずれか一つに記載の蛋白質加水分解物。
(5)加水分解が、100℃以上180℃以下、25時間以上で行われる、(4)に記載の蛋白質加水分解物。
(6)(1)〜(5)のいずれか一つに記載の蛋白質加水分解物を含有する、風味改良剤。
(7)(1)〜(5)のいずれか一つに記載の蛋白質加水分解物または(6)に記載の風味改良剤を含有させてなる、飲食品。
(8)(1)〜(5)のいずれか一つに記載の蛋白質加水分解物または(6)に記載の風味改良剤を飲食品に含有させる工程を含む、風味の改良された飲食品の製造方法。
(9)(1)〜(5)のいずれか一つに記載の蛋白質加水分解物または(6)に記載の風味改良剤を飲食品に含有させる工程を含む、飲食品の風味改良方法。
(10)風味改良剤としての、(1)〜(5)のいずれか一つに記載の蛋白質加水分解物の使用。
(11)(1)〜(5)のいずれか一つに記載の蛋白質加水分解物を飲食品に含有させる、(10)に記載の使用。
According to the present invention, the following inventions are provided.
(1) A protein hydrolyzate containing D-proline or a salt thereof,
A protein hydrolyzate containing 0.5 parts by mass or more of D-proline with respect to 100 parts by mass of a solid content of the protein hydrolyzate excluding inorganic salts.
(2) The protein hydrolyzate according to (1), containing 1 part by mass or more of D-aspartic acid with respect to 100 parts by mass of the solid content of the protein hydrolyzate excluding inorganic salts.
(3) The protein hydrolyzate according to (1) or (2), containing 0.7 parts by mass or more of D-glutamic acid with respect to 100 parts by mass of the solid content of the protein hydrolyzate excluding inorganic salts.
(4) The protein hydrolyzate according to any one of (1) to (3), wherein the protein hydrolyzate is a protein obtained by hydrolyzing a protein in a solution having a hydrogen ion concentration of 5 mol / L or more. object.
(5) The protein hydrolyzate according to (4), wherein the hydrolysis is performed at 100 ° C. or higher and 180 ° C. or lower for 25 hours or longer.
(6) A flavor improving agent containing the protein hydrolyzate according to any one of (1) to (5).
(7) A food or drink comprising the protein hydrolyzate according to any one of (1) to (5) or the flavor improving agent according to (6).
(8) A food / beverage product with improved flavor, comprising the step of incorporating the protein hydrolyzate according to any one of (1) to (5) or the flavor improver according to (6) into a food / beverage product. Production method.
(9) A method for improving the flavor of a food or drink, comprising the step of incorporating the protein hydrolyzate according to any one of (1) to (5) or the flavor improving agent according to (6) into the food or drink.
(10) Use of the protein hydrolyzate according to any one of (1) to (5) as a flavor improver.
(11) Use as described in (10) which makes food-drinks contain the protein hydrolyzate as described in any one of (1)-(5).

本発明によれば、飲食品において優れた風味改良効果を奏することができる。   ADVANTAGE OF THE INVENTION According to this invention, there exists an outstanding flavor improvement effect in food-drinks.

発明の具体的説明Detailed description of the invention

本明細書において、「D−アミノ酸」とはアミノ酸の立体異性体のうちD−体を意味する。本発明におけるD−プロリン、D−アスパラギン酸またはD−グルタミン酸は、それぞれのアミノ酸のD−体である。   In the present specification, “D-amino acid” means a D-isomer among stereoisomers of amino acids. D-proline, D-aspartic acid or D-glutamic acid in the present invention is a D-form of each amino acid.

蛋白質加水分解物
本発明の蛋白質加水分解物は、少なくともD−プロリンまたはその塩を含有することを一つの特徴としている。本発明の蛋白質加水分解物における無機塩を除いた蛋白質加水分解物の固形分(以下、「非無機塩性固形分」ともいう)あたりのD−プロリン自体の含有量は、乾燥質量基準で、非無機塩性固形分100質量部に対し、好ましくは0.5質量部以上100質量部以下であり、より好ましくは1質量部以上95質量部以下であり、さらに好ましくは、1.5質量部以上95質量部以下であり、さらに一層好ましくは2質量部以上95質量部以下であり、特に好ましくは4質量部以上95質量部以下である。
Protein hydrolyzate The protein hydrolyzate of the present invention is characterized by containing at least D-proline or a salt thereof. The content of D-proline per solid content (hereinafter also referred to as “non-inorganic salt solid content”) of the protein hydrolyzate excluding inorganic salts in the protein hydrolyzate of the present invention is on a dry mass basis, Preferably it is 0.5 mass part or more and 100 mass parts or less with respect to 100 mass parts of non-inorganic salt solid content, More preferably, it is 1 mass part or more and 95 mass parts or less, More preferably, it is 1.5 mass parts. It is 95 mass parts or less, More preferably, it is 2 mass parts or more and 95 mass parts or less, Especially preferably, they are 4 mass parts or more and 95 mass parts or less.

本発明の蛋白質加水分解物中の非無機塩性固形分の質量%は、蛋白質加水分解物の全固形分(質量%)から無機塩含量(質量%)を引くことにより容易に算出することができる。ここで、蛋白質加水分解物の全固形分(質量%)は、例えば、加熱乾燥式水分計等によって定量することができる。   The mass% of the non-inorganic salt solid content in the protein hydrolyzate of the present invention can be easily calculated by subtracting the inorganic salt content (mass%) from the total solid content (mass%) of the protein hydrolyzate. it can. Here, the total solid content (mass%) of the protein hydrolyzate can be quantified by, for example, a heat-drying moisture meter or the like.

また、蛋白質加水分解物の無機塩含量(質量%)は、例えば、無機塩が塩化ナトリウムの場合、電位差自動滴定装置等によって定量することができる。無機塩としては、例えば、塩化アンモニウム、硫酸銅、塩化ナトリウム、塩化カルシウム、酢酸ナトリウム、炭酸ナトリウム、硫酸バリウム、リン酸カルシウム、硫酸水素ナトリウム、リン酸二水素ナトリウム、炭酸水素ナトリウム、リン酸水素二ナトリウム、塩化水酸化カルシウム、塩化水酸化マグネシウム、塩化マグネシウム、硫酸ナトリウム、等が挙げられる。   Further, the inorganic salt content (mass%) of the protein hydrolyzate can be quantified by, for example, an automatic potentiometric titrator when the inorganic salt is sodium chloride. Examples of inorganic salts include ammonium chloride, copper sulfate, sodium chloride, calcium chloride, sodium acetate, sodium carbonate, barium sulfate, calcium phosphate, sodium hydrogen sulfate, sodium dihydrogen phosphate, sodium hydrogen carbonate, disodium hydrogen phosphate, Examples thereof include calcium chloride hydroxide, magnesium chloride hydroxide, magnesium chloride, sodium sulfate, and the like.

また、本発明の蛋白質加水分解物中のD−プロリンをはじめとするD−アミノ酸の含有量は、例えば、ガスクロマトグラフィー等(たとえば、分析方法についてはH.Frank,G.J.Nicholson,E.Bayer: J.Chromatogr.Sci.,15,174(1977)Rapid gas chromatographic separation of amino acid enantiomers with a novel chiral stationary phase.記載の方法)によって定量することができる。   In addition, the content of D-amino acids including D-proline in the protein hydrolyzate of the present invention is, for example, gas chromatography and the like (for example, H. Frank, GJ Nicholson, E. Bayer: J. Chromatogr. Sci., 15, 174 (1977) Rapid gas chromatographic separation of amino acid enantiomers with a novel chiral stationary phase.

本発明の蛋白質加水分解物は、全窒素が1質量%である場合において、D−プロリンを0.05質量%以上8.2質量%以下、好ましくは0.1質量%以上8.2質量%以下、より好ましくは0.15質量%以上8.2質量%以下、さらに好ましくは0.2質量%以上6.25質量%以下、特に好ましくは0.25質量%以上5.55質量%以下含有する。   In the protein hydrolyzate of the present invention, when the total nitrogen is 1% by mass, D-proline is 0.05% by mass or more and 8.2% by mass or less, preferably 0.1% by mass or more and 8.2% by mass. Or less, more preferably 0.15% by mass or more and 8.2% by mass or less, further preferably 0.2% by mass or more and 6.25% by mass or less, and particularly preferably 0.25% by mass or more and 5.55% by mass or less. To do.

また、本発明の蛋白質加水分解物はD−アスパラギン酸を含んでいることが好ましい。
本発明の蛋白質加水分解物における無機塩を除いた蛋白質加水分解物の固形分あたりのD−アスパラギン酸自体の含有量は、乾燥質量基準で、無機塩を除いた蛋白質加水分解物の固形分100質量部に対し、好ましくは1質量部以上95質量部以下であり、より好ましくは1.1質量部以上95質量部以下であり、さらに好ましくは1.3質量部以上95質量部以下であり、特に好ましくは1.5質量部以上95質量部以下である。
The protein hydrolyzate of the present invention preferably contains D-aspartic acid.
The content of D-aspartic acid itself per solid content of the protein hydrolyzate excluding the inorganic salt in the protein hydrolyzate of the present invention is 100% solid content of the protein hydrolyzate excluding the inorganic salt on a dry mass basis. Preferably, it is 1 part by mass or more and 95 parts by mass or less, more preferably 1.1 parts by mass or more and 95 parts by mass or less, and further preferably 1.3 parts by mass or more and 95 parts by mass or less, with respect to parts by mass. Particularly preferably, it is 1.5 parts by mass or more and 95 parts by mass or less.

また、本発明の蛋白質加水分解物は、全窒素が1質量%である場合において、D−アスパラギン酸を、好ましくは0.07質量%以上9.5質量%以下であり、より好ましくは0.10質量%以上9.5質量%以下、さらに好ましくは0.11質量%以上6.25質量%以下、特に好ましくは0.12質量%以上5.55質量%以下含有する。   In the protein hydrolyzate of the present invention, when the total nitrogen is 1% by mass, D-aspartic acid is preferably 0.07% by mass to 9.5% by mass, and more preferably 0.8%. 10 mass% or more and 9.5 mass% or less, More preferably, it is 0.11 mass% or more and 6.25 mass% or less, Most preferably, it contains 0.12 mass% or more and 5.55 mass% or less.

また、本発明の蛋白質加水分解物はD−グルタミン酸を含んでいることが好ましい。本発明の蛋白質加水分解物における無機塩を除いた蛋白質加水分解物の固形分あたりのD−グルタミン酸自体の含有量は、乾燥質量基準で、無機塩を除いた蛋白質加水分解物の固形分100質量部に対し、好ましくは0.7質量部以上94質量部以下であり、より好ましくは1質量部以上94質量部以下であり、さらに好ましくは1.5質量部以上94質量部以下であり、特に好ましくは2質量部以上94質量部以下である。   The protein hydrolyzate of the present invention preferably contains D-glutamic acid. The content of D-glutamic acid per solid content of the protein hydrolyzate excluding the inorganic salt in the protein hydrolyzate of the present invention is 100 masses of the solid content of the protein hydrolyzate excluding the inorganic salt on a dry mass basis. Is preferably 0.7 parts by weight or more and 94 parts by weight or less, more preferably 1 part by weight or more and 94 parts by weight or less, still more preferably 1.5 parts by weight or more and 94 parts by weight or less. Preferably they are 2 to 94 mass parts.

また、本発明の蛋白質加水分解物は、全窒素が1質量%である場合において、D−グルタミン酸を、好ましくは0.05質量%以上10.5質量%以下、より好ましくは0.07質量%以上10.5質量%以下、さらに好ましくは0.1質量%以上6.25質量%以下、特に好ましくは0.15質量%以上5.55質量%以下含有する。   Further, the protein hydrolyzate of the present invention, when the total nitrogen is 1% by mass, is preferably D-glutamic acid, preferably 0.05% by mass to 10.5% by mass, more preferably 0.07% by mass. The content is 10.5% by mass or less, more preferably 0.1% by mass or more and 6.25% by mass or less, and particularly preferably 0.15% by mass or more and 5.55% by mass or less.

本発明の蛋白質加水分解物におけるD−アミノ酸(D−プロリン、D−アスパラギン酸およびD−グルタミン酸)の塩は、飲食品への添加が許容される塩であれば特に制限されず、例えば、酸付加塩、金属塩、アンモニウム塩、有機アミン付加塩またはアミノ酸付加塩等が挙げられる。   The salt of D-amino acid (D-proline, D-aspartic acid and D-glutamic acid) in the protein hydrolyzate of the present invention is not particularly limited as long as it is a salt that can be added to foods and drinks. Examples include addition salts, metal salts, ammonium salts, organic amine addition salts, amino acid addition salts, and the like.

また、酸付加塩としては、塩酸塩、硫酸塩、硝酸塩、リン酸塩等の無機酸塩、酢酸塩、マレイン酸塩、フマル酸塩、クエン酸塩、リンゴ酸塩、乳酸塩、α−ケトグルタル酸塩、グルコン酸塩またはカプリル酸塩等の有機酸塩が挙げられる。   Acid addition salts include inorganic acid salts such as hydrochloride, sulfate, nitrate and phosphate, acetate, maleate, fumarate, citrate, malate, lactate, α-ketoglutar Organic acid salts such as acid salts, gluconates or caprylates.

また、金属塩としては、ナトリウム塩、カリウム塩等のアルカリ金属塩、マグネシウム塩、カルシウム塩等のアルカリ土類金属塩、アルミニウム塩または亜鉛塩等が挙げられる。   Examples of the metal salt include alkali metal salts such as sodium salt and potassium salt, alkaline earth metal salts such as magnesium salt and calcium salt, aluminum salt and zinc salt.

また、アンモニウム塩としては、アンモニウム、テトラメチルアンモニウム等の塩があげられる。有機アミン付加塩としては、モルホリンまたはピペリジン等の塩が挙げられる。   Examples of ammonium salts include ammonium and tetramethylammonium salts. Examples of organic amine addition salts include salts such as morpholine and piperidine.

また、アミノ酸付加塩としては、グリシン、フェニルアラニン、リジン、アスパラギン酸またはグルタミン酸等の塩が挙げられる。   Examples of amino acid addition salts include salts of glycine, phenylalanine, lysine, aspartic acid or glutamic acid.

本発明の蛋白質加水分解物の原料となる蛋白質は、特に限定されず、アミノ酸またはペプチドから合成された蛋白質であってもよいし、生物由来のものを用いてもよい。
上記蛋白質は、好ましくは生物由来の蛋白質であり、より好ましくは植物由来、動物由来または微生物由来(酵母または乳酸菌等)であり、さらに好ましくは動物由来であり、さらに一層好ましくはゼラチンまたはコラーゲンである。ゼラチンまたはコラーゲンをはじめとする蛋白質の由来する動物は、特に限定されず、例えば、牛、豚、羊、山羊、鶏、家鴨、鴨、鯨、硬骨魚類、軟骨魚類(サメ、エイ等)等が挙げられるが、好ましくは牛、豚または軟骨魚類であり、より好ましくは豚である。
The protein used as the raw material of the protein hydrolyzate of the present invention is not particularly limited, and may be a protein synthesized from an amino acid or a peptide, or a biological one may be used.
The protein is preferably a biological protein, more preferably a plant-derived, animal-derived, or microorganism-derived (yeast or lactic acid bacterium), more preferably an animal-derived, and still more preferably gelatin or collagen. . The animal from which the protein such as gelatin or collagen is derived is not particularly limited, and examples thereof include cattle, pigs, sheep, goats, chickens, domestic duck, duck, whales, teleosts, cartilaginous fish (sharks, rays, etc.). Among them, cows, pigs or cartilaginous fish are preferable, and pigs are more preferable.

本発明において蛋白質を加水分解する方法は、特に限定されず、例えば、アルカリによる加水分解、酸による加水分解、酵素による加水分解等が挙げられるが、好ましくは酸加水分解である。   In the present invention, the method for hydrolyzing the protein is not particularly limited, and examples thereof include alkali hydrolysis, acid hydrolysis, enzyme hydrolysis, and the like, and acid hydrolysis is preferable.

酸による加水分解時に用いる酸の種類は、特に限定されず、例えば、塩酸、硫酸、酢酸または乳酸等が挙げられるが、蛋白質の加水分解のし易さ等を考慮すると、好ましくは硫酸または塩酸であり、より好ましくは塩酸である。   The type of acid used at the time of hydrolysis with an acid is not particularly limited, and examples thereof include hydrochloric acid, sulfuric acid, acetic acid, and lactic acid. In consideration of easiness of protein hydrolysis, sulfuric acid or hydrochloric acid is preferable. Yes, more preferably hydrochloric acid.

また、酸による加水分解時の酸濃度は、特に限定されないが、好ましくは水素イオン濃度として、5mol/L以上である。より具体的には、酸として塩酸を用いた場合、塩酸の濃度としては5mol/L以上である。
かかる加水分解の溶液に用いられる媒体は、水等の水性媒体が挙げられる。
The acid concentration during hydrolysis with an acid is not particularly limited, but is preferably 5 mol / L or more as the hydrogen ion concentration. More specifically, when hydrochloric acid is used as the acid, the concentration of hydrochloric acid is 5 mol / L or more.
Examples of the medium used for the hydrolysis solution include aqueous media such as water.

また、酸による加水分解時の反応温度は、特に限定されないが、好ましくは100℃以上であり、より好ましくは100℃以上180℃以下であり、さらに好ましくは105℃以上160℃以下である。   The reaction temperature during hydrolysis with an acid is not particularly limited, but is preferably 100 ° C or higher, more preferably 100 ° C or higher and 180 ° C or lower, and further preferably 105 ° C or higher and 160 ° C or lower.

酸による加水分解の時間は、特に限定されないが、好ましくは、25時間以上であり、より好ましくは、5時間以上72時間以下であり、さらに好ましくは30時間以上72時間以下である。   The time for hydrolysis with an acid is not particularly limited, but is preferably 25 hours or more, more preferably 5 hours or more and 72 hours or less, and further preferably 30 hours or more and 72 hours or less.

上記条件は、D−プロリン、D−アスパラギン酸もしくはD−グルタミン酸またはそれらの塩を高レベルで含有する蛋白質加水分解物を提供するために好ましい。   The above conditions are preferable for providing a protein hydrolyzate containing a high level of D-proline, D-aspartic acid or D-glutamic acid or a salt thereof.

また、本発明の蛋白質加水分解物は、ラセマーゼ等の酵素を用いてD−プロリン、D−アスパラギン酸またはD−グルタミン酸の含有量を増加させて用いることもでき、本発明にはかかる態様も包含される。   The protein hydrolyzate of the present invention can also be used by increasing the content of D-proline, D-aspartic acid or D-glutamic acid using an enzyme such as racemase, and the present invention includes such an embodiment. Is done.

本発明の蛋白質加水分解物を風味改良効果が求められている飲食品に添加することにより、飲食品の風味を改良することができる。したがって、本発明の好ましい態様によれば、蛋白質加水分解物を含有する風味改良剤が提供される。また、別の好ましい態様によれば、風味改良剤としての、上記蛋白質加水分解物の使用が提供される。   By adding the protein hydrolyzate of the present invention to a food or drink for which a flavor improving effect is required, the taste of the food or drink can be improved. Therefore, according to the preferable aspect of this invention, the flavor improving agent containing a protein hydrolyzate is provided. Moreover, according to another preferable aspect, use of the said protein hydrolyzate as a flavor improving agent is provided.

本発明における風味改良の効果は、特に限定されないが、例えば、飲食品の甘味の向上、味のまとまりの向上、濃厚さの向上、こく味の向上、持続性の向上、煮込み感の向上、卵感の向上、油脂感の向上、香辛料感の向上、およびチーズ感の向上等が挙げられ、好ましくは、味のまとまりの向上、濃厚さの向上、こく味の向上、持続性の向上、卵感の向上、香辛料感の向上またはチーズ感の向上であり、より好ましくはこく味の向上、持続性の向上、香辛料感の向上またはチーズ感の向上である。したがって、一つの態様によれば、本発明の風味改良剤は、こく味向上剤、持続性の向上剤、香辛料感の向上剤またはチーズ感向上剤である。   The effect of improving the flavor in the present invention is not particularly limited. For example, the sweetness of food and drink is improved, the taste is improved, the richness is improved, the kokumi is improved, the sustainability is improved, the feeling of stew is improved, and the egg Improvement of feeling, improvement of oil and fat feeling, improvement of spice feeling, improvement of cheese feeling, and the like, preferably improvement of taste unity, improvement of richness, improvement of kokumi, improvement of sustainability, feeling of egg Improvement, improvement of spice feeling or improvement of cheese feeling, more preferably improvement of body taste, improvement of sustainability, improvement of spice feeling or improvement of cheese feeling. Therefore, according to one aspect, the flavor improving agent of the present invention is a kokumi improving agent, a sustaining improving agent, a spice improving agent, or a cheese feeling improving agent.

また、本発明の風味改良剤は、本発明の蛋白質加水分解物をそのまま風味改良剤として用いてもよいが、他の食品衛生上許容可能な添加物をさらに含んでいてもよい。例えば、飲食品の風味の効果的な改良を勘案して、D−アミノ酸またはその塩等を単独で、または二種以上組み合わせてさらに含有させてもよい。そして、本発明の好ましい態様によれば、風味改良剤は、D−プロリン、D−アスパラギン酸およびD−グルタミン酸からなる群から選択されるアミノ酸をさらに含んでなる。   Further, the flavor improving agent of the present invention may use the protein hydrolyzate of the present invention as it is as a flavor improving agent, but may further contain other food hygiene acceptable additives. For example, in consideration of effective improvement of the flavor of food and drink, D-amino acids or salts thereof may be further contained alone or in combination of two or more. And according to the preferable aspect of this invention, a flavor improving agent further comprises the amino acid selected from the group which consists of D-proline, D-aspartic acid, and D-glutamic acid.

本発明の風味改良剤におけるD−プロリン、D−アスパラギン酸およびD−グルタミン酸自体の含有量、全窒素含有量、全窒素1質量%当たり当該D−アミノ酸含有量は、添加対象の飲食品の種類または性質に応じて適宜決定してよいが、好ましくは上述した蛋白質加水分解物と同様とされる。   The content of D-proline, D-aspartic acid and D-glutamic acid itself, the total nitrogen content, and the D-amino acid content per 1% by mass of total nitrogen in the flavor improving agent of the present invention are the types of foods and beverages to be added. Alternatively, it may be appropriately determined depending on the properties, but is preferably the same as the protein hydrolyzate described above.

また、上記の他の食品衛生上許容可能な添加物としては、特に限定されないが、例えば、塩化ナトリウム等の無機塩、アスコルビン酸、フマル酸、リンゴ酸、酢酸、酒石酸、クエン酸、脂肪酸等のカルボン酸等の酸、L−グルタミン酸ナトリウム、L−グリシン、L−アラニン等のL−アミノ酸、イノシン酸ナトリウム、グアニル酸ナトリウム等の核酸、ショ糖、ブドウ糖、乳糖等の糖類、デキストリン、各種澱粉等の賦形剤、増粘剤(キサンタンガム、グァーガム、カラギーナン等)、pH調整剤、甘味料(スクラロース、ソーマチン、アセスルファムカリウム、アスパルテーム、キシリトール、カンゾウ抽出物、サッカリンおよびその塩、ステビア、ソルビトール等)、酸化防止剤(エリソルビン酸、カテキン、トコフェロール等)、着色料(アナトー色素、ウコン色素、β−カロチン等)、香料(アセト酢酸エチル、アセトフェノン、アニスアルデヒド等の合成香料、または天然香料)、保存料(安息香酸ナトリウム、しらこたん白抽出物、ソルビン酸カリウム、プロピオン酸カルシウム、ポリリジン等)、栄養強化剤(ビタミンA、ビタミンC等のビタミン類、塩化カルシウム等のミネラル類等)、水等の飲食品に使用可能な添加物、醤油、味噌、畜肉エキス、家禽エキス、魚介エキス、酵母エキス、蛋白質加水分解物等の天然調味料、スパイス類、ハーブ類等の香辛料、デキストリン、各種澱粉等の賦形剤等を含有してもよい。また、本発明の風味改良剤は、調味料として用いてもよい。   In addition, the other food hygiene acceptable additive is not particularly limited, for example, inorganic salts such as sodium chloride, ascorbic acid, fumaric acid, malic acid, acetic acid, tartaric acid, citric acid, fatty acid, etc. Acids such as carboxylic acids, L-amino acids such as sodium L-glutamate, L-glycine and L-alanine, nucleic acids such as sodium inosinate and sodium guanylate, sugars such as sucrose, glucose and lactose, dextrin, various starches, etc. Excipients, thickeners (xanthan gum, guar gum, carrageenan, etc.), pH adjusters, sweeteners (sucralose, thaumatin, acesulfame potassium, aspartame, xylitol, licorice extract, saccharin and its salts, stevia, sorbitol, etc.), Antioxidants (erythorbic acid, catechin, tocopherol, etc.), wearing (Anato dye, turmeric dye, β-carotene, etc.), fragrance (synthetic fragrance such as ethyl acetoacetate, acetophenone, anisaldehyde, or natural fragrance), preservative (sodium benzoate, shirako protein extract, sorbic acid) Potassium, calcium propionate, polylysine, etc.), nutrient enhancers (vitamins such as vitamin A and vitamin C, minerals such as calcium chloride), additives that can be used for food and drink such as water, soy sauce, miso, livestock meat Extracts, poultry extracts, seafood extracts, yeast extracts, natural seasonings such as protein hydrolysates, spices such as spices and herbs, dextrins, excipients such as various starches, and the like may also be included. Moreover, you may use the flavor improving agent of this invention as a seasoning.

また、本発明の風味改良剤は、液状、粉状、顆粒状等のいずれの形状を有するものであってもよい。あるいは、油脂と乳化剤および必要に応じて増粘安定剤を添加して乳化することにより、乳液状とすることもできる。   Moreover, the flavor improving agent of this invention may have any shapes, such as liquid form, a powder form, and a granular form. Or it can also be made into emulsion by adding fats and oils, an emulsifier, and a thickening stabilizer as needed, and emulsifying.

本発明の風味改良剤中の蛋白質加水分解物の含有量は、特に規定されないが、例えば、0.5〜100質量%とすることができる。   The content of the protein hydrolyzate in the flavor improving agent of the present invention is not particularly defined, but can be, for example, 0.5 to 100% by mass.

飲食品
本発明の風味改良剤を風味改良効果が求められている飲食品に含有させることにより、飲食品の風味を改良することができる。したがって、本発明の一つの態様によれば、風味改良剤を含有させてなる、飲食品が提供される。
Food / beverage products By including the flavor improving agent of the present invention in a food / beverage product for which a flavor improvement effect is required, the flavor of the food / beverage products can be improved. Therefore, according to one aspect of the present invention, there is provided a food or drink product containing a flavor improver.

本発明の風味改良剤を飲食品に含有させる工程では、風味改良剤を飲食品の最終製品に加えても、飲食品の製造工程前および製造工程中に加えてもよく、飲食品の製造工程には加熱工程(例えば、煮込み工程)を含んでいてもよい。また、本発明の風味改良剤は、摂食時直前に飲食品に加えてもよい。さらに、本発明の風味改良剤を2回以上に分けて、飲食品に加えてもよい。   In the step of adding the flavor improver of the present invention to the food or drink, the flavor improver may be added to the final product of the food or drink, or may be added before and during the manufacturing process of the food or drink. May include a heating step (for example, a stew step). Moreover, you may add the flavor improving agent of this invention to food-drinks just before eating. Furthermore, you may add the flavor improving agent of this invention to food-drinks in 2 steps or more.

また、風味改良剤を飲食品へ含有させる手法としては、本発明の風味改良剤を飲食品に加えてもよいし、飲食品を本発明の風味改良剤に加えてもよく、または飲食品と、本発明の風味改良剤と混合して用いてもよい。飲食品を本発明の風味改良剤に加えるとは、例えば、本発明の風味改良剤が既に存在する容器等に、飲食品が加える態様も含まれる。   Moreover, as a method of including a flavor improver in a food or drink, the flavor improver of the present invention may be added to the food or drink, the food or drink may be added to the flavor improver of the present invention, or The flavor improving agent of the present invention may be used as a mixture. Adding food and drink to the flavor improver of the present invention includes, for example, a mode in which the food and drink is added to a container in which the flavor improver of the present invention already exists.

本発明の風味改良剤が添加される飲食品は、特にその種類は限定されず、液体、固体、または半固体のいずれの形態のものであってもよく、他の食品原料調味料または風味改良剤等が含有されてなる飲食品であってもよい。飲食品の具体的な例としては、飲料、マヨネーズ、ソース、漬物、たれ、インスタント食品、食パン、スープ、ラーメン、ビタミン剤等が挙げられるが、好適な例としては、カレー、ホワイトシチュー、グラタン、ラーメンスープ等の飲食品が挙げられる。   The food / beverage products to which the flavor improving agent of the present invention is added are not particularly limited, and may be in any form of liquid, solid, or semi-solid, and other food ingredient seasonings or flavor improvers. It may be a food or drink containing an agent or the like. Specific examples of the food and drink include beverages, mayonnaise, sauces, pickles, sauce, instant food, bread, soup, ramen, vitamins, etc., but suitable examples include curry, white stew, gratin, Food and drink such as ramen soup.

本発明の風味改良剤の飲食品への添加量は、特に限定されず、添加対象や、加水分解する蛋白質の種類、性質等に応じて当業者が適宜決定することができるが、例えば、飲食品100質量部に対して、蛋白質加水分解物(乾燥質量を基準)として0.01〜99質量部であり、好ましくは0.1〜50質量部である。   The addition amount of the flavor improving agent of the present invention to foods and drinks is not particularly limited, and can be appropriately determined by those skilled in the art depending on the addition target, the type of protein to be hydrolyzed, the nature, etc. It is 0.01-99 mass parts as a protein hydrolyzate (on the basis of dry mass) with respect to 100 mass parts of goods, Preferably it is 0.1-50 mass parts.

より具体的には、例えば、カレーソースの場合、風味改良効果の向上を勘案すれば、風味改良剤の飲食品への添加量は、蛋白質加水分解物として、好ましくはカレーソース100質量部当たり0.1質量部以上であり、より好ましくは、0.2質量部以上である。   More specifically, for example, in the case of curry sauce, if the improvement in flavor improvement is taken into account, the amount of the flavor improver added to the food or drink is preferably 0 as the protein hydrolyzate per 100 parts by weight of curry sauce. .1 part by mass or more, and more preferably 0.2 part by mass or more.

また、本発明の一つの態様によれば、本発明の蛋白質加水分解物を飲食品に含有させる工程を含む、風味が改良された飲食品の製造方法が提供される。   Moreover, according to one aspect of this invention, the manufacturing method of the food / beverage products with improved flavor including the process which contains the protein hydrolyzate of this invention in food / beverage products is provided.

また、本発明の別の態様によれば、本発明の蛋白質加水分解物を飲食品に含有させる工程を含む、飲食品の風味改良方法が提供される。   Moreover, according to another aspect of this invention, the flavor improvement method of food-drinks including the process of making the food-drinks contain the protein hydrolyzate of this invention is provided.

本発明の飲食品の製造方法および本発明の風味改良方法は、本発明の風味改良剤に関する上記記載に基づいて実施することができる。すなわち、本発明の飲食品の製造方法および本発明の風味改良方法における蛋白質加水分解物を飲食品に含有させる工程は、本発明の風味改良剤の使用態様に準じて実施することができる。すなわち、蛋白質加水分解物を加える時期、加える方法およびその飲食品に含有させる量は、本発明の風味改良剤と同じあってよい。   The manufacturing method of the food / beverage products of this invention and the flavor improvement method of this invention can be implemented based on the said description regarding the flavor improvement agent of this invention. That is, the step of incorporating the protein hydrolyzate into the food or drink in the method for producing the food or drink of the present invention and the flavor improving method of the present invention can be carried out according to the use mode of the flavor improver of the present invention. That is, the timing of adding the protein hydrolyzate, the method of adding the protein hydrolyzate, and the amount of the protein hydrolyzate may be the same as the flavor improving agent of the present invention.

なお、蛋白質加水分解物の他、飲食品に使用可能な他の添加物を飲食品へ含有させる場合には、他の添加物は蛋白質加水分解物と同時に飲食品に加えてもよく、別々に加えてもよい。さらに、蛋白質加水分解物を飲食品に使用可能な他の添加物と混合して、飲食品へ加えてもよい。   In addition to protein hydrolyzate, when other additives usable for food and drink are included in the food and drink, other additives may be added to the food and drink simultaneously with the protein hydrolyzate. May be added. Furthermore, you may mix a protein hydrolyzate with the other additive which can be used for food / beverage products, and may add to food / beverage products.

以下、実施例により本発明をより具体的に説明するが、本発明の技術範囲はこれらの例示に限定されるものではない。なお、以下、「%」とあるのは、特に断りがない限り、いずれも質量%である。また、本実施例では、非無機塩性固形分を非食塩性固形分で示す。
食塩含量は電位差測定で測定した。また、D−ProはD−プロリンを、D−AspはD−アスパラギン酸を、D−GluはD−グルタミン酸を示す。
EXAMPLES Hereinafter, although an Example demonstrates this invention more concretely, the technical scope of this invention is not limited to these illustrations. Hereinafter, “%” means mass% unless otherwise specified. Moreover, in a present Example, non-inorganic salt solid content is shown by non-salt solid content.
The salt content was measured by potentiometric measurement. D-Pro represents D-proline, D-Asp represents D-aspartic acid, and D-Glu represents D-glutamic acid.

実施例1
10mol/Lの塩酸60mLとゼラチン28g(乾燥減量87.5%)をメディウム瓶中で混合し、試験液を調製した。次に、加圧容器を用いて、125℃にて18時間(試験液1)および72時間(試験液2)の各時間、撹拌加熱処理を行った。試験液を冷した後、水酸化ナトリウム水溶液を用いてpH4に調整した。pHを調整した試験液1および2を、水分量が5質量%以下になるように、熱風乾燥し、各粉末1および2を得た。得た粉末について、分析を行った結果を表2に示す。非無機塩(食塩)性固形分あたりのD−プロリン量は、全固形分中から無機塩(食塩)換算質量を除いた値のうち、GC(検出器はFID)を用いて測定したD−プロリン量の質量比率を用いて定量した。全窒素は全質量あたりの質量%で示す(ケルダール法による)。なお、無機塩(食塩)量の定量は電位差自動滴定装置を用いて行った。
Example 1
60 mL of 10 mol / L hydrochloric acid and 28 g of gelatin (loss on drying 87.5%) were mixed in a medium bottle to prepare a test solution. Next, using a pressurized container, stirring heat treatment was performed at 125 ° C. for 18 hours (test solution 1) and 72 hours (test solution 2). The test solution was cooled and then adjusted to pH 4 using an aqueous sodium hydroxide solution. Test solutions 1 and 2 with adjusted pH were dried with hot air so that the water content was 5% by mass or less to obtain powders 1 and 2, respectively. Table 2 shows the results of analysis of the obtained powder. The amount of D-proline per non-inorganic salt (salt) solid content is D- measured using GC (detector is FID) out of the total solid content excluding the mass converted to inorganic salt (salt). It quantified using the mass ratio of the amount of proline. Total nitrogen is expressed as mass% per total mass (by Kjeldahl method). The amount of inorganic salt (salt) was quantified using a potentiometric automatic titrator.

Figure 0006275638
Figure 0006275638

実施例2:カレーソース(1)
市販のカレールウ 100gを温水700mLに混合し、カレーソースを得た。
上記カレーソースを100gずつパウチ袋に分注し、実施例1で得た粉末1および2を用いて表2の配合で試験区1および2のカレーソースを調製した。
Example 2: Curry sauce (1)
100 g of commercially available curry roux was mixed with 700 mL of warm water to obtain a curry sauce.
100 g of the above curry sauce was dispensed into pouch bags, and curry sauces in test sections 1 and 2 were prepared using the powders 1 and 2 obtained in Example 1 with the composition shown in Table 2.

Figure 0006275638
Figure 0006275638

調製した各パウチ袋を、シーラーで密閉し、沸騰浴にて密閉した袋のまま10分間加熱した。
加熱後、パウチ袋の内容物の風味等について、コントロールと比較して、パネラー5名で官能検査を行った。官能検査の各項目は下記のような評価基準で行った。
Each prepared pouch bag was sealed with a sealer and heated for 10 minutes with the bag sealed in a boiling bath.
After heating, the sensory test was performed by five panelists on the flavor of the contents of the pouch bag compared to the control. Each item of the sensory test was performed according to the following evaluation criteria.

[評価基準]
7:コントロールと比較して明らかに向上している
6:コントロールと比較して向上している
5:コントロールと比較してやや向上している
4:コントロールと同等
3:コントロールと比較してやや低下している
2:コントロールと比較して低下している
1:コントロールと比較して明らかに低下している
[Evaluation criteria]
7: Clearly improved compared to control 6: Improved compared to control 5: Slightly improved compared to control 4: Equivalent to control 3: Slightly decreased compared to control 2: Reduced compared to control 1: Clearly decreased compared to control

その結果の平均値を、表3に示す。

Figure 0006275638
The average value of the results is shown in Table 3.
Figure 0006275638

表3に示すとおり、粉末2を添加した試験区1のカレーソース、ならびにD−プロリンおよび粉末1を添加した試験区2のカレーソースは、コントロールと比較して持続性、および持続性の関与するこく味(以下、同じ)、香辛料感が向上していた。比較区1では、コントロールと比べ、そのような傾向はほとんど見られなかった。   As shown in Table 3, the curry sauce of test section 1 to which powder 2 was added and the curry sauce of test section 2 to which D-proline and powder 1 were added were persistent and involved in sustainability compared to the control. The kokumi (hereinafter the same) and spices were improved. In Comparative Zone 1, such a tendency was hardly seen as compared with the control.

実施例3:カレーソース(2)
市販のカレールウ 100gを温水700mLに混合し、カレーソースを得た。
上記カレーソースを100gずつパウチ袋に分注し実施例1で得た粉末1および2を用いて表4の配合で各試験区を調製した。
Example 3: Curry sauce (2)
100 g of commercially available curry roux was mixed with 700 mL of warm water to obtain a curry sauce.
100 g of the curry sauce was dispensed into pouch bags, and each test section was prepared using the powders 1 and 2 obtained in Example 1 with the composition shown in Table 4.

Figure 0006275638
Figure 0006275638

調製したパウチ袋を、シーラーで密閉し、沸騰浴にて密閉した袋のまま10分間加熱した。
加熱後、各パウチ袋の内容物の風味について、コントロールと比較して、パネラー5名で官能検査を行った。官能検査の各項目は実施例2と同じ評価基準で行った。その結果の平均値を、表5に示す。
The prepared pouch bag was sealed with a sealer and heated for 10 minutes with the bag sealed in a boiling bath.
After heating, the sensory test was performed by five panelists on the flavor of the contents of each pouch bag compared to the control. Each item of the sensory test was performed according to the same evaluation criteria as in Example 2. The average value of the results is shown in Table 5.

Figure 0006275638
Figure 0006275638

表5に示すとおり、粉末2を添加した試験区3のカレーソース、ならびに、D−プロリン、D-アスパラギン酸、D-グルタミン酸および粉末1を添加した試験区4のカレーソースは、コントロールと比較して持続性、こく味および香辛料感が向上していた。比較区2では、コントロールと比べ、そのような傾向はほとんど見られなかった。   As shown in Table 5, the curry sauce of test group 3 to which powder 2 was added and the curry sauce of test group 4 to which D-proline, D-aspartic acid, D-glutamic acid and powder 1 were added were compared with the control. Sustainability, richness and spice feeling were improved. In Comparative Group 2, such a tendency was hardly seen as compared with the control.

実施例4:グラタンソース(1)
薄力粉 43.7gを無塩バター 4.37gで焦がさないように炒めた後、沸騰直前まで温めた成分無調整牛乳874gを少量ずつ添加した。食塩3.1g、ホワイトペッパー0.7g、ゴーダチーズ34.9gを袋に入れ、混合し、上記の薄力粉、無塩バター、牛乳に添加した。添加後、合計質量の850gになるまで煮詰めてグラタンソースを調製した。調製したグラタンソースを100gずつパウチ袋に分注し、実施例1で得た粉末1および2を用いて表6の配合で、各試験区のグラタンソースを調製した。
Example 4: Gratin sauce (1)
After fried 43.7 g of the flour at 437 g of unsalted butter to avoid scorching, 874 g of component-unadjusted milk warmed until just before boiling was added in small portions. 3.1 g of salt, 0.7 g of white pepper, and 34.9 g of Gouda cheese were put in a bag, mixed, and added to the above-described flour, unsalted butter, and milk. After the addition, the gratin sauce was prepared by boiling until the total mass reached 850 g. 100 g of the prepared gratin sauce was dispensed into a pouch bag, and using the powders 1 and 2 obtained in Example 1, the gratin sauce of each test section was prepared with the composition shown in Table 6.

Figure 0006275638
食塩添加量は、粉末1および2の無機塩(食塩)含有量から計算して、各区の無機塩(食塩)濃度が同等となるよう調整した。
粉末(g/100g)は、グラタンソース100gに対する添加量を示す。
ppm=質量/質量(グラタンソース100gに対してのD−プロリン添加量)
Figure 0006275638
The salt addition amount was calculated from the inorganic salt (salt) content of powders 1 and 2, and adjusted so that the inorganic salt (salt) concentration in each section was equivalent.
Powder (g / 100g) shows the addition amount with respect to 100g of gratin sauces.
ppm = mass / mass (addition amount of D-proline to 100 g of gratin sauce)

調製したパウチ袋を、シーラーで密閉し、沸騰浴にて密閉した袋のまま10分間加熱した。
加熱後、各グラタンソースの風味について、コントロールと比較して、パネラー3名で官能検査を行った。官能検査の各項目は実施例2と同じ評価基準で行った。
その結果の平均値を、表7に示す。
The prepared pouch bag was sealed with a sealer and heated for 10 minutes with the bag sealed in a boiling bath.
After heating, the sensory test was performed by three panelists on the flavor of each gratin sauce compared to the control. Each item of the sensory test was performed according to the same evaluation criteria as in Example 2.
The average value of the results is shown in Table 7.

Figure 0006275638
Figure 0006275638

表7に示すとおり、粉末2を添加した試験区5のグラタンソース、ならびに、D−プロリンおよび粉末1を添加した試験区6のグラタンソースは、コントロールと比較して持続性、こく味、チーズ感が向上していた。比較区3では、コントロールと比べ、そのような傾向はほとんど見られなかった。   As shown in Table 7, the gratin sauce in test group 5 to which powder 2 was added and the gratin sauce in test group 6 to which D-proline and powder 1 were added had durability, richness and cheese feeling as compared to the control. Had improved. In Comparative Zone 3, such a tendency was hardly seen as compared with the control.

実施例5:グラタンソース(2)
薄力粉 43.7gを無塩バター 4.37gで焦がさないように炒めた後、沸騰直前まで温めた成分無調整牛乳874gを少量ずつ添加した。食塩3.1g、ホワイトペッパー0.7g、ゴーダチーズ34.9gを袋に入れ、混合し、上記の薄力粉、無塩バター、牛乳に添加した。添加後、合計質量の850gになるまで煮詰めてグラタンソースを調製した。調製したグラタンソースを100gずつパウチ袋に分注し、実施例1で得た粉末1および2を用いて表8の配合で、各試験区のグラタンソースを調製した。
Example 5: Gratin sauce (2)
After fried 43.7 g of the flour at 437 g of unsalted butter to avoid scorching, 874 g of component-unadjusted milk warmed until just before boiling was added in small portions. 3.1 g of salt, 0.7 g of white pepper, and 34.9 g of Gouda cheese were put in a bag, mixed, and added to the above-described flour, unsalted butter, and milk. After the addition, the gratin sauce was prepared by boiling until the total mass reached 850 g. 100 g of the prepared gratin sauce was dispensed into a pouch bag, and using the powders 1 and 2 obtained in Example 1, the gratin sauce of each test section was prepared with the composition shown in Table 8.

Figure 0006275638
食塩添加量は、粉末1および2の食塩含有量から計算して、各区の食塩濃度が同等となるよう調整した。
粉末(g/100g)は、グラタンソース100gに対する添加量を示す。
ppm=質量/質量(グラタンソース100gに対してのD−アミノ酸それぞれの添加量)
Figure 0006275638
The salt addition amount was calculated from the salt content of powders 1 and 2, and adjusted so that the salt concentration in each section was equal.
Powder (g / 100g) shows the addition amount with respect to 100g of gratin sauces.
ppm = mass / mass (addition amount of each D-amino acid to 100 g of gratin sauce)

調製したパウチ袋を、シーラーで密閉し、沸騰浴にて密閉した袋のまま10分間加熱した。
加熱後、各パウチ袋の内容物の風味について、コントロールと比較して、パネラー5名で官能検査を行った。官能検査の各項目は実施例2と同じ評価基準で行った。
その結果の平均値を、表9に示す。
The prepared pouch bag was sealed with a sealer and heated for 10 minutes with the bag sealed in a boiling bath.
After heating, the sensory test was performed by five panelists on the flavor of the contents of each pouch bag compared to the control. Each item of the sensory test was performed according to the same evaluation criteria as in Example 2.
The average value of the results is shown in Table 9.

Figure 0006275638
Figure 0006275638

表9に示すとおり、粉末2を添加した試験区7のグラタンソース、ならびにD−プロリン、D-アスパラギン酸、D-グルタミン酸および粉末1を添加した試験区8のグラタンソースは、コントロールと比較して持続性、こく味、チーズ感が向上していた。比較区4では、コントロールと比べ、そのような傾向はほとんど見られなかった。   As shown in Table 9, the gratin sauce of test group 7 to which powder 2 was added and the gratin sauce of test group 8 to which D-proline, D-aspartic acid, D-glutamic acid and powder 1 were added were compared with the control. Sustainability, rich taste, and cheese feeling were improved. In Comparative Group 4, such a tendency was hardly seen as compared with the control.

Claims (8)

D−プロリンまたはその塩を含有するゼラチン加水分解物であって、無機塩を除いた前記ゼラチン加水分解物の固形分100質量部に対してD−プロリンを0.5質量部以上含有するゼラチン加水分解物を含有する、風味改良剤。 A D- proline or gelatin hydrolyzate containing a salt thereof, gelatin hydrolyzate containing D- proline least 0.5 parts by mass with respect to 100 parts by mass of the solid content of the gelatine hydrolyzate excluding inorganic salt A flavor improver containing a degradation product. 前記ゼラチン加水分解物が、無機塩を除いた前記ゼラチン加水分解物の固形分100質量部に対してD-アスパラギン酸を1質量部以上含有する、請求項1に記載の風味改良剤。 The gelatin hydrolyzate, contains more than 1 part by mass of D- aspartic acid relative to 100 parts by weight of the solid content of the gelatine hydrolyzate excluding inorganic salt, flavor improving agent according to claim 1. 前記ゼラチン加水分解物が、無機塩を除いた前記ゼラチン加水分解物の固形分100質量部に対してD−グルタミン酸を0.7質量部以上含有する、請求項1または2に記載の風味改良剤。 The gelatin hydrolyzate, contains more than 0.7 parts by mass of D- glutamic against 100 parts by weight of the solid content of the gelatine hydrolyzate excluding inorganic salt, flavor improving agent according to claim 1 or 2 . 無機塩を除いたゼラチン加水分解物の固形分100質量部に対して、D−プロリンを0.5質量部以上含有し、かつ、D-アスパラギン酸を1質量部以上含有する、ゼラチン加水分解物であって、ゼラチンを水素イオン濃度5mol/L以上の溶液中で加水分解して得られる、ゼラチン加水分解物を含有する、風味改良剤The solid content 100 parts by weight of the gelatine hydrolyzate excluding inorganic salt, a D- proline containing more than 0.5 part by weight, and containing D- aspartate 1 part by mass or more, gelatine hydrolyzate a is, gelatin obtained by hydrolyzing a hydrogen ion concentration 5 mol / L or more in the solution and contain gelatin hydrolyzate, flavor improving agents. 無機塩を除いた前記ゼラチン加水分解物の固形分100質量部に対してD−グルタミン酸を0.7質量部以上含有する、請求項4に記載のゼラチン加水分解物を含有する、風味改良剤The flavor improving agent containing the gelatin hydrolyzate of Claim 4 which contains 0.7 mass part or more of D-glutamic acid with respect to 100 mass parts of solid content of the said gelatin hydrolyzate except the inorganic salt. 前記加水分解が、100℃以上180℃以下で25時間以上行われる、請求項4または5に記載のゼラチン加水分解物を含有する、風味改良剤The flavor improving agent containing the gelatin hydrolyzate of Claim 4 or 5 with which the said hydrolysis is performed at 100 degreeC or more and 180 degrees C or less for 25 hours or more. 求項1〜のいずれか一項に記載の風味改良剤を飲食品に含有させる工程を含む、風味の改良された飲食品の製造方法。 Motomeko 1 comprising the step of incorporating the flavor improving agent according to any one of 6 to food or drink, the flavor improved process for the preparation of food and drink. 求項1〜のいずれか一項に記載の風味改良剤を飲食品に含有させる工程を含む、飲食品の風味改良方法。 Motomeko 1 the flavor improving agent according to any one of 6, including a step to be contained in food or drink, a food and beverage flavor improvement methods.
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