CN107183356A - The preparation method of bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine - Google Patents
The preparation method of bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine Download PDFInfo
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
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Abstract
The invention discloses the preparation method of bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine, it is related to agriculture and animal husbandry livestock and poultry technical field.Preparation method is：The preparation of beef-protein medium, the preparation of potato culture, the preparation of czapek's medium, activation, the culture of strain：Raw material for bacterium peptide feed of the fermenting and producing containing taurine is uniformly mixed; in 110 DEG C~130 DEG C high-temp steam sterilizings; it is solid fermentation thing after cooling; by the seed bacteria suspension prepared in step 2; respectively it is inoculated into by 4%~8% volume ratio in the solid fermentation thing for bacterium of having gone out; 65h~70h is cultivated, the bacterium peptide feed rich in taurine is produced.Tunning contains multiple polypeptides there is provided abundant nutrition and promotes fully absorbing for nutrition.As the sesame seed meal of fermentation substrate, wide material sources are cheap.Therefore the bacterium peptide feed containing taurine can substitute fish meal completely or partially, so as to reduce feed cost.
Description
Technical field：
The present invention relates to the preparation method of bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine, belong to farming animals
Animal husbandry technical field.
Background technology：
Feed manufacturing industry dog-eat-dog, feed price remains high, and for example dregs of beans, fish meal are in short supply causes valency for routine protein raw material
Lattice rise steadily, and feed cost, which sharply increases, cultivate income constantly reduces, and how to reduce feed cost as market extensive concern
The problem of.The most frequently used method for reducing cost is come instead of parts of traditional quality using more economical sesame seed meal, cotton dregs etc.
Higher dregs of beans and fish meal.
The plant-derived albumens such as sesame seed meal, cotton dregs lack taurine this important substance compared with fish meal, and for livestock and poultry fish
For class animal, taurine is conditionally essential amino acid because its it is spontaneous can only synthesize 30%~40% needed for its physiology,
So a part must be supplemented from food.Taurine, which has, improves growth of animals or poultry, reproductive performance, improves its immunity, promotes
The biological functions such as feeding.Research shows, lack the plant-derived albumen bait of taurine can cause fish body occur it is slow-growing and
Some pathological phenomenons, such as green liver syndrome and hemolytic anemia.
China is the most country of world's Sesame Seed Yield, and sesame seed meal is the accessory substance after sesame oil expression, and cost is than dregs of beans also
Will low 20%-30%.Its crude protein is up to 45% or so, and methionine content is higher by dregs of beans 40%, and methionine exactly taurine
The precursor substance of route of synthesis.The bacterium peptide feed of multi-strain solid-state fermented Semen Sesami dregs of rice production, can solve the problem that the trophism abdomen of animal
Rush down；Conditioning and the overall activity of active cell and body, promote the gastrointestinal function of animal, improve anti-disease resistance against diseases；Also
A large amount of active probiotics can be supplemented, strengthen beneficial microorganism growth and breeding, suppress the harmful bacterias such as Escherichia coli, salmonella
Growth, improves the microecological balance of enteron aisle, reduces the generation of disease；The immunologic function of animal body is improved, antibiotic is reduced
Consumption；Nutriment Neng Bei roads mucous membrane therein directly absorbs, and absorption rate is fast, nutritious and raising immunity dual-use function.
Meanwhile, acted on by microbial fermentation, can effectively reduce ANFs in dregs of rice class.
With the development of biotechnology, microbe fermentation technique degrading plant protein resource prepares the feeding of active peptide albumen
Material, the important means of the bioactivity as research small peptide, makes it possible efficiently and safely to obtain active peptides products.Sesame
Cake protein is the peptide resources bank of a very abundant, and small peptide feed and additive are prepared with sesame seed meal, will turn into and improve sesame pair
Added value of product, improves livestock and poultry production performance, improves an effective way of livestock and poultry quality.Prepared by microbial fermentation new
The feeding active small peptide product of green, is highly beneficial to improving Animal nutrition and promoting the sound development of aquaculture.
The content of the invention：
In view of the above-mentioned problems, being rich in ox sulphur the technical problem to be solved in the present invention is to provide the production of multi-strain fermentation sesame seed meal
The preparation method of the bacterium peptide feed of acid.
The preparation method of bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine of the present invention be：Step 1:
The preparation of beef-protein medium：Accurately weigh beef extract 3g, peptone 5g, sodium chloride 10g to be put into beaker, add
1000mL water is stirred with glass bar, then adjusts pH 7.0~7.2；
The preparation of potato culture：Peeling potatoes, are cut into block and add water and boil 30min, wherein potato:Water=1:4
~6, then with filtered through gauze, 20g glucose is added, water is supplied after stirring to 1000mL；
The preparation of czapek's medium：It is accurate weigh sodium nitrate 2g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g,
Ferrous sulfate 0.01g, sucrose 30g are put into beaker, are added 1000mL water and are stirred with glass bar.
Step 2: the activation of strain, culture, by bacillus subtilis YS-45, yeast S-78, aspergillus niger PL-39 activation
Strain is separately added into beef-protein medium, potato culture and the Cha Shi culture of 15mL~20mL steps one preparation
Base, is made bacteria suspension, then bacteria suspension is expanded into culture 12h~14h in 30 DEG C~32 DEG C, 250r/min shaking tables, will after centrifugation
Supernatant is removed, that is, respectively obtains bacillus subtilis YS-45 seeds bacteria suspension, yeast S-78 seeds bacteria suspension and aspergillus niger
PL-39 seed bacteria suspensions；
Step 3：By the raw material for bacterium peptide feed of the fermenting and producing containing taurine：Sesame seed meal, wheat bran, molasses, phosphoric acid hydrogen
Dipotassium, calcium chloride are uniformly mixed, in 110 DEG C~130 DEG C high-temp steam sterilizing 25min~35min, are sent out after cooling for solid
Ferment thing, the bacillus YS-45 prepared respectively in step 2, yeast S-78 and aspergillus niger PL-39 seed bacteria suspensions are respectively pressed
4%~8% volume ratio is inoculated into the solid fermentation thing sterilized, adds the 35%~40% of above-mentioned raw materials gross mass
Water, stirs, and then cultivates 65h~70h under the conditions of 30 DEG C~32 DEG C in solid-state fermenter, produces rich in taurine
Bacterium peptide feed.
As preferred, it is used to produce bacterium peptide feeding of the multi-strain fermentation sesame seed meal production rich in taurine in described step three
Material includes the raw material of following mass fraction：7-9 parts of sesame seed meal, 0.5-1.5 parts of wheat bran, 0.3-0.7 parts of molasses, dipotassium hydrogen phosphate
0.1-0.4 parts, 0.1-0.4 parts of calcium chloride.
Beneficial effects of the present invention：Bacillus subtilis YS-45 contains protease, lipase, amylase, can decompose sesame
Numb cake protein, produces small-molecule active substance, has stronger two packing spaces ability in animal intestinal tract.Yeast S-78, which has, to be promoted
Growth characteristics, intracellular containing abundant protein, vitamin, amino acid, wherein protein accounts for more than the 50% of its dry weight, energy
Substantial amounts of thalline nutrition is provided for animal, alcohols material can be produced during the fermentation, the local flavor of feed can be increased, improves suitable
Mouth property, while can improve the micro-ecological environment of animal intestinal tract, improves animal immunizing power；Aspergillus niger PL-39 can secrete generation plant
Sour enzyme and cellulase, can effectively degrade the ANFs such as phytic acid, cellulose.And three kinds of bacterium are acted on the equal nonhazardous of animal.
Three's collective effect produces polytype enzyme, and the generation of fermentative degradation sesame seed meal is rich in the bacterium peptide feed of taurine, and taurine is to adjust
The active material of body normal physiological activity is saved, with maintaining normal vision function, maintaining body osmotic balance, cell is adjusted
The advantages of calcium balance, and taurine is relevant with the mobility of cell membrane, can hypoglycemic, regulation nerve conduction, participation endocrine work
Dynamic, regulation lipid digestion is with absorbing, increase contractile ability, improve body immunity, enhancing cell membrane oxidation resistance,
Protect the biological action extensively such as cardiac muscle cell.Tunning contains multiple polypeptides there is provided abundant nutrition and promotes filling for nutrition
Divide and absorb.And as the sesame seed meal of fermentation substrate, wide material sources are cheap.Therefore the bacterium peptide feed containing taurine can be complete
Or part substitutes fish meal, so as to reduce feed cost.
Brief description of the drawings：
For ease of explanation, the present invention is described in detail by following specific implementations and accompanying drawing.
Fig. 1 is the high-efficient liquid phase chromatogram of taurine standard items in the embodiment of the present invention.
Fig. 2 is the height for being rich in taurine in taurine bacterium peptide feed that the embodiment of the present invention 1 is prepared in solid-state fermenter
Effect liquid phase chromatogram figure.
Fig. 3 substitutes the experimental data figure that fish meal influences on grass carp production performance for bacterium peptide feed in the embodiment of the present invention 2.
Embodiment：
Below in conjunction with specific embodiment, to bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine of the present invention
Preparation method be described in further detail.
Embodiment 1：Bacterium peptide feed of the multi-strain fermentation production rich in taurine includes the raw material of following mass fraction：Sesame
9 parts of the dregs of rice, 0.5 part of wheat bran, 0.3 part of molasses, 0.1 part of dipotassium hydrogen phosphate, 0.1 part of calcium chloride.
The preparation method step of bacterium peptide feed of the multi-strain fermentation production rich in taurine be：
Step 1: the preparation of beef-protein medium：Accurately beef extract 3g, peptone 5g, sodium chloride 10g is weighed to put
Enter in beaker, add 1000mL water and stirred with glass bar, then adjust pH 7.0；
The preparation of potato culture：Peeling potatoes, are cut into block and add water and boil 30min, wherein potato:Water=1:4,
Then filtered through gauze is used, 20g glucose is added, water is supplied after stirring to 1000mL；
The preparation of czapek's medium：It is accurate weigh sodium nitrate 2g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g,
Ferrous sulfate 0.01g, sucrose 30g are put into beaker, are added 1000mL water and are stirred with glass bar.
Step 2: the activation of strain, culture：By bacillus subtilis YS-45, yeast S-78, aspergillus niger PL-39 activation
Strain is separately added into beef-protein medium, potato culture and the czapek's medium of 15mL steps one preparation, is made
Bacteria suspension, then bacteria suspension is expanded into culture 12h in 30 DEG C, 250r/min shaking tables, supernatant is removed after centrifugation, i.e., obtained respectively
To bacillus subtilis YS-45, yeast S-78 and aspergillus niger PL-39 seed bacteria suspension；
Step 3：The raw material of the bacterium peptide feed rich in taurine will be produced for multi-strain fermentation：Sesame seed meal, wheat bran and
After the trophic factors such as molasses, dipotassium hydrogen phosphate, calcium chloride are weighed in proportion, it is uniformly mixed, high-temperature steam goes out at 110 DEG C
Bacterium 25min, is solid fermentation thing after cooling, by the bacillus YS-45 prepared respectively in step 2, yeast S-78 and aspergillus niger
PL-39 seed bacteria suspensions, are respectively inoculated into the solid fermentation thing sterilized by 8% volume ratio, add the total matter of above-mentioned raw materials
40% water of amount, stirs, then cultivates 70h under the conditions of 30 DEG C in solid-state fermenter, produce the bacterium rich in taurine
Peptide feed.
The detection of content of taurine：Bacterium peptide feed 30g rich in taurine prepared by Example 1 is dissolved in 50mL water, from
The heart takes supernatant, plus equivalent volumes trichloroacetic acid removing protein, and equivalent ether defatting freezes, takes 5mg freeze-dried powders to be dissolved in 100 μ L water,
300 μ L derivating agents, derivative 30min are added, then is freezed, taurine is detected using high performance liquid chromatography, Fig. 2 is richness in embodiment
The high-efficient liquid phase chromatogram of taurine in bacterium peptide feed containing taurine.
Imported fish meal content of taurine 1% or so, domestic fish meal content of taurine 0.5% or so is real shown in reference picture 2
Apply in example 1 that content of taurine is that 3.57% (i.e. the content of taurine is in 100g dries in the bacterium peptide feed rich in taurine
3.57g)。
By embodiment 1 it can be seen that using content of taurine ratio in the bacterium peptide feed rich in taurine of the method preparation
Content of taurine in imported fish meal is high.
Embodiment 2：Experiment is randomly divided into 4 using single factor experiment design, the grass carp for being about 10g from original body mass
Processing, each 3 repetitions of processing are each to repeat 20 tail fishes, and cultivation place aquaculture Research Base in Changsha University enters
OK, indoor circulation temperature-controlled glass aquarium specification is 120cm × 80cm × 80cm, carries out the experiment of 60 days by a definite date.Feeding 3 daily
It is secondary, respectively 8:00、12:00、16:00.Fish meal consumption is on the basis of 51% in control group of formula, respectively with 24%,
34% and 44% bacterium peptide feed replaces in formula 16%, 23% and 31% fish meal consumption, and research bacterium peptide feed replaces fish meal
Influence to Growth of Grass Carps Ctenopharyngodon Idellus performance (testing index includes survival rate, rate of body weight gain, feed coefficient and specific growth rate).
It is rate of body weight gain, feed coefficient, specific from figure 3, it can be seen that relative to control group, the culture efficiency of test group 1,2,3 is good
Growth rate etc. is all improved significantly, wherein replacing 23% imported fish meal, best results with 34% bacterium peptide feed.
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (2)
1. the preparation method of bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine, it is characterised in that：Described system
Preparation Method is comprised the steps of：Step 1: the preparation of beef-protein medium：It is accurate weigh beef extract 3g, peptone 5g,
Sodium chloride 10g is put into beaker, is added 1000mL water and is stirred with glass bar, then adjusts pH 7.0~7.2；
The preparation of potato culture：Peeling potatoes, are cut into block and add water and boil 30min, wherein potato:Water=1:4~6,
Then filtered through gauze is used, 20g glucose is added, water is supplied after stirring to 1000mL；
The preparation of czapek's medium：Accurately weigh sodium nitrate 2g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, sulfuric acid
Ferrous 0.01g, sucrose 30g are put into beaker, are added 1000mL water and are stirred with glass bar；
Step 2: the activation of strain, culture：By bacillus subtilis YS-45, yeast S-78, aspergillus niger PL-39 activated spawn
It is separately added into beef-protein medium, potato culture and the czapek's medium of the preparation of 15mL~20mL steps one, system
Expand culture 12h~14h in 30 DEG C~32 DEG C, 250r/min shaking tables into bacteria suspension, then by bacteria suspension, by supernatant after centrifugation
Remove, that is, respectively obtain bacillus subtilis YS-45 seeds bacteria suspension, yeast S-78 seeds bacteria suspension and aspergillus niger PL-39 kinds
Daughter bacteria suspension；
Step 3：By the raw material for bacterium peptide feed of the fermenting and producing containing taurine：Sesame seed meal, wheat bran and molasses, phosphoric acid hydrogen
After dipotassium, calcium chloride are weighed, it is uniformly mixed, in 110 DEG C~130 DEG C high-temp steam sterilizing 25min~35min, after cooling
For solid fermentation thing, by the seed bacteria suspension prepared in step 2, respectively consolidating for bacterium of having gone out is inoculated into by 4%~8% volume ratio
In body fermentate, 35%~40% water of above-mentioned raw materials gross mass is added, is stirred, then 30 in solid-state fermenter
DEG C~32 DEG C under the conditions of cultivate 65h~70h, produce the bacterium peptide feed rich in taurine.
2. according to the preparation method of bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine described in claim 1,
It is characterized in that：Included in described step three for producing bacterium peptide feed of the multi-strain fermentation sesame seed meal production rich in taurine
The raw material of following mass fraction：7-9 parts of sesame seed meal, 0.5-1.5 parts of wheat bran, 0.3-0.7 parts of molasses, dipotassium hydrogen phosphate 0.1-0.4
Part, 0.1-0.4 parts of calcium chloride.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331302A (en) * | 2021-05-14 | 2021-09-03 | 山东大益生物科技集团有限公司 | Fermented feed containing sesame oil residue and preparation method thereof |
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| CN1537445A (en) * | 2003-09-22 | 2004-10-20 | 湖南师范大学 | Method for producing forage protein contg. blood powder |
| CN102919565A (en) * | 2012-09-18 | 2013-02-13 | 沈阳巨源源生物科技有限公司 | Meat chick granulated feed containing active bacterium fermentation ingredient and traditional Chinese medicine ingredient |
| CN103609862A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Method for improving feeding nutritive value of sesame seed meal by combining enzymolysis method with fermentation method |
| CN106720929A (en) * | 2016-11-29 | 2017-05-31 | 长沙学院 | For the preparation method of bacterium peptide rice feed of the fermenting and producing rich in taurine |
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2017
- 2017-06-05 CN CN201710411390.5A patent/CN107183356A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537445A (en) * | 2003-09-22 | 2004-10-20 | 湖南师范大学 | Method for producing forage protein contg. blood powder |
| CN102919565A (en) * | 2012-09-18 | 2013-02-13 | 沈阳巨源源生物科技有限公司 | Meat chick granulated feed containing active bacterium fermentation ingredient and traditional Chinese medicine ingredient |
| CN103609862A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Method for improving feeding nutritive value of sesame seed meal by combining enzymolysis method with fermentation method |
| CN106720929A (en) * | 2016-11-29 | 2017-05-31 | 长沙学院 | For the preparation method of bacterium peptide rice feed of the fermenting and producing rich in taurine |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331302A (en) * | 2021-05-14 | 2021-09-03 | 山东大益生物科技集团有限公司 | Fermented feed containing sesame oil residue and preparation method thereof |
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Application publication date: 20170922 |