CN107183320A - Application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared - Google Patents
Application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared Download PDFInfo
- Publication number
- CN107183320A CN107183320A CN201710296996.9A CN201710296996A CN107183320A CN 107183320 A CN107183320 A CN 107183320A CN 201710296996 A CN201710296996 A CN 201710296996A CN 107183320 A CN107183320 A CN 107183320A
- Authority
- CN
- China
- Prior art keywords
- powder
- euphausia superba
- fancy carp
- vitamin
- hyperchromic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses euphausia superba powder prepare the hyperchromic feed of fancy carp in application and the hyperchromic feed of fancy carp.The hyperchromic feed is made up of the component of following mass ratio：Euphausia superba powder 5~72%, FSPC 1~20%, barley worm powder 1~5%, fly maggot powder 1~5%, Self- raising flour 5~24%, beer yeast powder 2~10%, starch 1~10%, fairy shrimp 4~20%, spirulina 2~4%, soybean lecithin 1~4%, glycine betaine 2~4%, fish oil 1~3%, vitamin mixtures 1~2.5%, mineral element mixture 1~5%, immunopotentiator 1~5%, lactic acid bacillus 0.5~5%, lilac 1~3%, Rhodopseudomonas palustris 0.1~1%, Paprika 1~3%.The hyperchromic feed has the hyperchromic effect of good color development to fancy carp, and fish body edible 5 days can be in gorgeous color and colour-fast, and attractant is good, can improve immunity, anti-stress ability and survival rate.
Description
Technical field
The invention belongs to technical field of aquaculture.The hyperchromic feed of fancy carp is being prepared more particularly, to euphausia superba powder
The application of aspect.
Background technology
Fancy carp body length is up to 1~1.5 meter, and athletic looking, beautiful in colour, decorative pattern are changeable, stroke is male so, have high view and admire
And Feeding value, it is a kind of high-grade ornamental fish of the fashionable world today, there is " funny fellow's stone in water ", the U.S. of " the natatory art work "
Claim.
Therefore, the cultivation of fancy carp has important economic benefit, and is used as a kind of important fancy fishes, it is necessary to fish
Body progress is hyperchromic, so that fish body shows more bright-coloured beautiful color, the need for satisfaction is viewed and admired.
In addition, euphausia superba powder has preferably effect in terms of aquatic feeds, it is that one kind has before larger exploitation
The high-quality resource of scape.But euphausia superba powder Oil repellent is high, this is also the problem of its maximum is most difficult to overcome place, and limiting it should
With.
The content of the invention
The technical problem to be solved in the present invention be overcome the shortcomings of in existing fancy carp breeding process the hyperchromic technology of color development and
The problem of euphausia superba powder Oil repellent is high, is prepared for the euphausia superba powder that Oil repellent is less than 1mg/kg, and then a kind of brocade of optimization
The special hyperchromic feed of carp, has the hyperchromic effect of good color development to fancy carp, and edible 5 days or so of fish body can in gorgeous color and not
Fade, body line is graceful, color is beautiful, and there is excellent food calling performance to fancy carp, easy to digest, not polluted water, it is possible to increase
Immunity, anti-stress ability and survival rate, significantly improve the economic benefit of fancy carp cultivation, with good application prospect.
It is an object of the invention to provide application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared, especially low fluorine south
Pole krill meal.
Another object of the present invention is to provide a kind of special hyperchromic feed of fancy carp.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
Application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared.
It is further preferred that the Oil repellent of the euphausia superba powder is 0~1mg/kg.
It is further preferred that the euphausia superba powder is prepared by the following method：
S1. krill keeps 5~15min, then be dipped in pH 4.6~5.0 after pretreatment in 80~85 DEG C of water
Distilled water in, ultrasound carries out pre- defluorinate processing；
S2. defluorinate processing is carried out using modified bagasse powder：Modified bagasse powder is added according to 15~20g/L ratio
In distilled water, regulation pH is 4.5~5.0, and krill is immersed, and is reacted under ultrasound condition；
S3. the krill after step S2 processing is soaked into 20~30min in 75~85 DEG C of distilled water, then 40~
45 DEG C of drying to water content is 20~40%, is 10~15% then at 50~55 DEG C of drying to water content；
S4. the krill of drying is crushed to fineness for 120~220 mesh, it is 1~5% then to dry to water content, is obtained
To low fluorine euphausia superba powder, Oil repellent is below 1mg/kg.
Wherein, the preparation method of modified bagasse powder is described in step S2：
S21. pulverized and sieved by bagasse drying or after drying, obtain bagasse powder；
S22. bagasse powder is soaked after 6~7h in 20~30% sodium hydroxide solutions, washing, filtering；
S23. the product after S22 is handled is soaked in 1.5~2g/L liquor potassic permanganates, 50~80 DEG C reaction 1.5~
3h；
S24. the product after S23 is handled is washed, filters, dried, and produces modified bagasse powder.
Thaw, screen, clean furthermore it is preferred that being pre-processed described in step S1 and including hydrostatic successively.Wherein, screen preferred
Want the full krill of screening high-quality in ground.
Preferably, condition ultrasonic described in step S1 is the ultrasonically treated 3~5h of 80~150W.
It is highly preferred that every 1 hour of step S1 ultrasonically treated periods changed first water.
Preferably, condition ultrasonic described in step S2 is the ultrasonically treated 2~3.5h of 80~150W.
In addition, the baking step of krill uses two step oven drying methods in step S3, krill meal yield can be significantly improved.
Not only Oil repellent is very low for low fluorine euphausia superba powder prepared by the above method, and very limits remain krill
Active material and nutriment.The application of modified bagasse powder simultaneously also achieves twice laid and its value is reclaimed.
A kind of special hyperchromic feed of fancy carp, is made up of the component of following mass percent：It is euphausia superba powder 5~72%, big
Beans protein concentrate 1~20%, barley worm powder 1~5%, fly maggot powder 1~5%, Self- raising flour 5~24%, beer yeast powder 2~
10%th, starch 1~10%, fairy shrimp 4~20%, spirulina 2~4%, soybean lecithin 1~4%, glycine betaine 2~4%, fish oil 1
~3%, vitamin mixtures 1~2.5%, mineral element mixture 1~5%, immunopotentiator 1~5%, lactic acid bacillus
0.5~5%, lilac 1~3%, Rhodopseudomonas palustris 0.1~1%, Paprika 1~3%.
Preferably, the special hyperchromic feed of described fancy carp is made up of the component of following mass percent：By following quality hundred
The component of ratio is divided to be made：Euphausia superba powder 10~40%, FSPC 10~20%, barley worm powder 3~4%, fly maggot powder 3
~4%, Self- raising flour 10~20%, beer yeast powder 5~8%, starch 5~8%, fairy shrimp 8~15%, spirulina 2~
4%th, soybean lecithin 2~4%, glycine betaine 2~4%, fish oil 1~2.5%, vitamin mixtures 1~2.5%, mineral element are mixed
Compound 2~5%, immunopotentiator 1~5%, lactic acid bacillus 1~3%, lilac 2~3%, Rhodopseudomonas palustris 0.5
~1%, Paprika 1.5~2.5%.
It is highly preferred that the special hyperchromic feed of described fancy carp is made up of the component of following mass percent：Euphausia superba powder
30%th, FSPC 6%, barley worm powder 4%, fly maggot powder 4%, Self- raising flour 10%, beer yeast powder 5%, starch
7%th, fairy shrimp 8%, spirulina 3%, soybean lecithin 3%, glycine betaine 3%, fish oil 2%, vitamin mixtures 2%, mineral member
It is plain mixture 3%, immunopotentiator 2.5%, lactic acid bacillus 2%, lilac 2.5%, Rhodopseudomonas palustris 1%, red
Green pepper powder 2%.
Wherein it is preferred to, the euphausia superba powder is low fluorine euphausia superba powder, and Oil repellent is less than 1mg/kg.
It is highly preferred that method is made low fluorine euphausia superba powder by mentioned earlier.
The fly maggot powder is made by raw material of the fly maggot propagated artificially obtained by powder.
Preferably, the immunopotentiator is：β -1,3 glucans and/or β -1,6 glucans.
Preferably, the vitamin mixtures include：Vitamin A, vitamine D3, vitamin E, Vitamin K3, vitamin
B1, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, calcium pantothenate, biotin, inositol, folic acid, vitamin C.
It is highly preferred that weight ratio of each vitamin component in total feed is as follows：35~45g/kg vitamin As, 2~4g/
Kg vitamine D3s, 65~75g/kg vitamin Es, 5~8g/kg Vitamin K3s, 35~45g/kg vitamin B1s, 2~5g/kg dimension lifes
Plain B2,60~80g/kg vitamin B6s, 5~7g/kg vitamin B12s, 20~40g/kg nicotinic acid, 20~40g/kg calcium pantothenates, 0.2
~0.25g/kg biotins, 180~280g/kg inositols, 5~8g/kg folic acid, 300~500g/kg vitamin Cs.
Preferably, the mineral element mixture includes：Copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, cobalt chloride, Asia
Sodium selenate, potassium dihydrogen phosphate, sodium dihydrogen phosphate.
It is highly preferred that weight ratio of each vitamin component in total feed is as follows：20~40g/kg of copper sulphate, ferrous sulfate
40~50g/kg, 10~25g/kg of zinc sulfate, 5~10g/kg of manganese sulfate, 0.2~0.5g/kg of cobalt chloride, sodium selenite 0.2~
0.5g/kg, 15~20g/kg of potassium dihydrogen phosphate, 15~20g/kg of sodium dihydrogen phosphate.
In the formula of the special hyperchromic feed of fancy carp of the present invention, material is feed grade, described mineral element mixture
It is that its component is well mixed.Described vitamin mixtures are that its component is well mixed.
Specifically, the preparation method of the special hyperchromic feed of fancy carp is as follows：
(1) according to the content of each component in formula, each component is weighed；
(2) by euphausia superba powder, FSPC, barley worm powder, fly maggot powder, beer yeast powder, starch, Self- raising flour
With the mixing of mineral element mixture, 50~250 mesh are crushed to, are stirred for uniformly, obtaining powder；
(3) fairy shrimp, spirulina, lilac are homogenized to 50~250 mesh through colloid mill, then wherein add glycine betaine,
Soybean lecithin, fish oil, vitamin mixtures, lactic acid bacillus, immunopotentiator, Rhodopseudomonas palustris and Paprika, enter
Row emulsification homogenate, obtains emulsion；
(4) powder of step (2) and the emulsion of step (3) are mixed, adds water and size mixing, water is raw material gross mass
30~50%, through roller drying, drying temperature is 100~200 DEG C, then vacuum dried, and vacuum is 60~90kPa, cold again
But the hyperchromic feed of finished product is obtained afterwards.
The present invention has shown that a kind of modified bagasse is used to handle krill, and pin by substantial amounts of research and exploration
Preparation to euphausia superba powder, especially in terms of focusing on reduction Oil repellent and ensureing the reservation to greatest extent of active material, takes
Obtained preferable achievement.The Preference and nutritional need that have studied different fish are taken into account simultaneously, substantial amounts of screening study work has been done
Make, have developed a kind of special hyperchromic feed of fancy carp, there is good color development hyperchromic effect and attractant to fancy carp fancy carp.
The invention has the advantages that：
Low fluorine euphausia superba powder Oil repellent prepared by the present invention can be less than 1mg/kg, for the development and application of euphausia superba powder
With significant meaning.And the twice laid of bagasse, value recovery are also achieved, with important economic value and environmental protection
Meaning.
The special hyperchromic feed of fancy carp that the present invention is provided has the hyperchromic effect of good color development to fancy carp, and fish body is edible 5 days
Left and right can be in gorgeous color and colour-fast, and fine figure, color are beautiful, and sight is good.
Meanwhile, the hyperchromic feed has excellent food calling performance to fancy carp, easy to digest, is remarkably improved the immune of fancy carp
Power, anti-stress ability and survival rate；And raw material sources are reliable, not polluted water, steady quality, safety, feed manipulation is easy, can
The economic benefit of fancy carp cultivation is improved, with good application prospect.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment, but embodiment does not do any type of to the present invention
Limit.Unless stated otherwise, the reagent of the invention used, method and apparatus is the art conventional reagent, methods and apparatus.
Unless stated otherwise, following examples agents useful for same and material are purchased in market.
Embodiment 1 prepares low fluorine euphausia superba powder
1st, modified bagasse powder is prepared：
(1) bagasse powder is pulverized and sieved to obtain by bagasse drying or after drying；
(2) bagasse powder is soaked after 6.5h in 25% sodium hydroxide solution, washing, filtering；
(3) it is soaked in again in 1.7g/L liquor potassic permanganates, 60 DEG C of reaction 2h；
(4) product after (3) are handled, washs, filters, dries, produce modified bagasse powder.
2nd, low fluorine euphausia superba powder is prepared：
(1) after krill thaws through hydrostatic, screened, cleans, 10min is kept in 80 DEG C of water, then be dipped in pH 4.8 steaming
In distilled water, the ultrasonically treated 4h of 100W carry out pre- defluorinate processing, change first water within every 1 hour during processing；
(2) defluorinate processing is carried out using modified bagasse powder：Ratio according to 20g/L, which adds modified bagasse powder, distills
In water, regulation pH is 4.5, and krill is immersed, 3h is reacted under 100W ultrasound conditions；
(3) krill after step (2) processing is soaked into 25min in 80 DEG C of distilled water, then dried extremely for 45 DEG C
Water content is 30%, is 10% then at 55 DEG C of drying to water content；
(4) krill of drying is crushed to fineness for 120~220 mesh, it is 1~5% then to dry to water content, is obtained
To low fluorine euphausia superba powder.
3rd, by experimental test verification, the Oil repellent of euphausia superba powder is less than 1mg/kg, and shrimp med yield can reach 90%
More than.Moreover, can also very limits reservation krill bioactive substance and nutriment.
The low fluorine euphausia superba powder optimum preparation condition of embodiment 2
1st, influence of the modified bagasse powder to euphausia superba powder Oil repellent
(1) influence of the concentration of sodium hydroxide solution to Oil repellent in krill meal
According to the method that modified bagasse powder is prepared in embodiment 1, using concentration of sodium hydroxide solution as single argument, one is prepared
Series modified bagasse powder.A series of euphausia superba powders are obtained according still further to the method that euphausia superba powder is prepared in embodiment 1.Determine
Oil repellent in euphausia superba powder, calculates shrimp med yield.
As a result as shown in table 1, when concentration of sodium hydroxide solution is 25~35%, the Oil repellent of euphausia superba powder is lower.
Influence of the concentration of sodium hydroxide solution to Oil repellent in krill meal when the bagasse powder of table 1 is modified
(2) influence of the liquor potassic permanganate concentration to Oil repellent in krill meal
According to the method that modified bagasse powder is prepared in embodiment 1, using liquor potassic permanganate concentration as single argument, one is prepared
Series modified bagasse powder.A series of euphausia superba powders are obtained according still further to the method that euphausia superba powder is prepared in embodiment 1.Determine
Oil repellent in euphausia superba powder, calculates shrimp med yield.
As a result as shown in table 2, when liquor potassic permanganate concentration is 1.5~2.2g/L, the Oil repellent of euphausia superba powder is more
It is low.
Influence of the liquor potassic permanganate concentration to Oil repellent in krill meal when the bagasse powder of table 2 is modified
| KMnO4Solution concentration | Shrimp med Oil repellent | Shrimp med yield |
| 1g/L | 10mg/kg | 91% |
| 1.1g/L | 12mg/kg | 92% |
| 1.3g/L | 5mg/kg | 94% |
| 1.5g/L | 1mg/kg | 95% |
| 1.7g/L | 0.5mg/kg | 97% |
| 1.9g/L | 0.6mg/kg | 96% |
| 2.0g/L | 0.9mg/kg | 93% |
| 2.2g/L | 1mg/kg | 91% |
| 2.4g/L | 23mg/kg | 90% |
(3) influence of the NaOH solution soak time to Oil repellent in krill meal
According to the method that modified bagasse powder is prepared in embodiment 1, using sodium hydroxide solution soak time as single argument, system
A series of standby modified bagasse powder.A series of euphausia superba powders are obtained according still further to the method that euphausia superba powder is prepared in embodiment 1.
Oil repellent in euphausia superba powder is determined, shrimp med yield is calculated.
As a result as shown in table 3, when sodium hydroxide solution soak time is 5.5~7.5h, the Oil repellent of euphausia superba powder
It is lower.
Influence of the NaOH solution soak time to Oil repellent in krill meal when the bagasse powder of table 3 is modified
| NaOH solution soak time | Shrimp med Oil repellent | Shrimp med yield |
| 4.0h | 2.6mg/kg | 91% |
| 5.0h | 3mg/kg | 93% |
| 5.5h | 1.1mg/kg | 92% |
| 6.0h | 1.2mg/kg | 95% |
| 6.5h | 0.6mg/kg | 96% |
| 7.0h | 0.8mg/kg | 97% |
| 7.5h | 1mg/kg | 90% |
| 8.0h | 3.1mg/kg | 92% |
| 9.0h | 3.0mg/kg | 91% |
(4)KMnO4Solution soaks influence of the reaction temperature to Oil repellent in krill meal
According to the method that modified bagasse powder is prepared in embodiment 1, reaction temperature is soaked to be monotropic using liquor potassic permanganate
Amount, prepares a series of modified bagasse powder.A series of South Pole phosphorus are obtained according still further to the method that euphausia superba powder is prepared in embodiment 1
Shrimp med.Oil repellent in euphausia superba powder is determined, shrimp med yield is calculated.
As a result as shown in table 4, when liquor potassic permanganate immersion reaction temperature is 40~80 DEG C, the fluorine of euphausia superba powder contains
Amount is lower.
The KMnO when bagasse powder of table 4 is modified4Solution soaks influence of the reaction temperature to Oil repellent in krill meal
(5) in addition, by multifactor Variable Selection, as a result showing, bagasse powder method of modifying condition is：Sodium hydroxide is molten
Liquid concentration is 20~30%, and soak time soaks 6~7h；Liquor potassic permanganate concentration is 1.5~2g/L, 50~80 DEG C of reactions
1.5~3h.Optimal bagasse powder method of modifying condition is as shown in the Examples.
2nd, influence of the euphausia superba powder defluorinate condition to krill meal Oil repellent
(1) krill pre-processes the influence to krill meal Oil repellent
Experiment display, after krill is thawed, should screen full, coloury krill, first be soaked after cleaning up with hot water
Bubble, water temperature is advisable with 80~85 DEG C, soaks 5~15min.
(2) influence of the pH value of pre- defluorinate processing distilled water used to euphausia superba powder Oil repellent
According to the method that euphausia superba powder is prepared in embodiment 1, the pH value of distilled water used is handled to be monotropic using pre- defluorinate
Amount, is made a series of euphausia superba powders.Oil repellent in euphausia superba powder is determined, shrimp med yield is calculated.
As a result as shown in table 5, when the pH value that pre- defluorinate handles distilled water used is 4.8~5.2, the fluorine of euphausia superba powder
Content is lower.
Influence of the pH value of the pre- defluorinate of table 5 processing distilled water used to krill meal Oil repellent
| The pH value of pre- defluorinate processing distilled water used | Shrimp med Oil repellent | Shrimp med yield |
| 2.0 | 3.6mg/kg | 92% |
| 3.0 | 3.1mg/kg | 90% |
| 4.0 | 3.5mg/kg | 91% |
| 4.2 | 2.3mg/kg | 92% |
| 4.4 | 1.9mg/kg | 93% |
| 4.6 | 2.1mg/kg | 94% |
| 4.8 | 0.7mg/kg | 95% |
| 5.0 | 0.8mg/kg | 93% |
| 5.2 | 0.6mg/kg | 92% |
| 5.4 | 2.4mg/kg | 94% |
| 5.6 | 2.6mg/kg | 93% |
| 5.8 | 2.2mg/kg | 90% |
| 6.0 | 2.5mg/kg | 91% |
(3) influence of the consumption of defluorinate processing modified bagasse powder used to euphausia superba powder Oil repellent
According to the method that euphausia superba powder is prepared in embodiment 1, using defluorinate handle the consumption of modified bagasse powder used as
Single argument, is made a series of euphausia superba powders.Oil repellent in euphausia superba powder is determined, shrimp med yield is calculated.
As a result as shown in table 6, when defluorinate processing modified bagasse powder consumption used is 15~20g/L, euphausia superba powder
Oil repellent it is lower.
Influence of the pH value of the pre- defluorinate of table 6 processing distilled water used to krill meal Oil repellent
| Modified bagasse powder consumption | Shrimp med Oil repellent | Shrimp med yield |
| 5g/L | 25mg/kg | 93% |
| 10g/L | 12mg/kg | 92% |
| 11g/L | 8mg/kg | 94% |
| 12g/L | 3mg/kg | 95% |
| 13g/L | 2mg/kg | 96% |
| 14g/L | 1.8mg/kg | 94% |
| 15g/L | 0.9mg/kg | 93% |
| 16g/L | 0.7mg/kg | 95% |
| 17g/L | 0.5mg/kg | 97% |
| 18g/L | 0.7mg/kg | 95% |
| 19g/L | 0.6mg/kg | 93% |
| 20g/L | 0.7mg/kg | 91% |
| 21g/L | 0.8mg/kg | 92% |
(4) influence of the pH value of solution to krill meal Oil repellent when the defluorinate of modified bagasse powder is handled
According to the method that euphausia superba powder is prepared in embodiment 1, the pH value of solution when being handled with the defluorinate of modified bagasse powder
For single argument, a series of euphausia superba powders are made.Oil repellent in euphausia superba powder is determined, shrimp med yield is calculated.
As a result as shown in table 7, when when the defluorinate of modified bagasse powder is handled, the pH value of solution is 4.5~5.0, krill
The Oil repellent of powder is lower.
Influence of the pH value of solution to krill meal Oil repellent when the modified bagasse powder defluorinate of table 7 is handled
(5) influence of the modified bagasse powder defluorinate processing reaction time to krill meal Oil repellent
According to the method that euphausia superba powder is prepared in embodiment 1, the reaction time is handled to be single using the defluorinate of modified bagasse powder
Variable, is made a series of euphausia superba powders.Oil repellent in euphausia superba powder is determined, shrimp med yield is calculated.
As a result as shown in table 8, when the modified bagasse powder defluorinate processing reaction time is 2~4, the fluorine of euphausia superba powder contains
Amount is lower.
The modified bagasse powder defluorinate of table 8 handles influence of the reaction time to krill meal Oil repellent
| The defluorinate of modified bagasse powder handles the reaction time | Shrimp med Oil repellent | Shrimp med yield |
| 0.5h | 26mg/kg | 90% |
| 1.0h | 15mg/kg | 91% |
| 1.5h | 8mg/kg | 92% |
| 2.0h | 2.0mg/kg | 93% |
| 2.5h | 0.9mg/kg | 94% |
| 3.0h | 0.7mg/kg | 95% |
| 3.5h | 0.5mg/kg | 93% |
| 4.0h | 0.6mg/kg | 92% |
| 4.5h | 1.6mg/kg | 94% |
| 5.0h | 3.5mg/kg | 93% |
(6) in addition, being shown by multifactor Variable Selection result, the preparation process condition of low fluorine euphausia superba powder is：In advance
The pH value of defluorinate processing distilled water used is 4.5~5.0, and defluorinate processing modified bagasse powder consumption used is 15~20g/L, is changed
Property bagasse powder defluorinate processing when solution pH value be 4.5~5.0, the modified bagasse powder defluorinate processing reaction time is 2~
3.5h when.The preparation process condition of optimal low fluorine euphausia superba powder is as described in Example 1.
3rd, defluorination process contrast test
(1) low fluorine euphausia superba powder is prepared, experiment is set：
Experimental group：Embodiment 1.
Contrast groups 1：The method of be the same as Example 1, difference is：In step (1), after krill screening, cleaning, omit
" the step of keeping 10min " in 80 DEG C of water.
Contrast groups 2：The method of be the same as Example 1, difference is：In step (1), the pre- defluorinate processing of distilled water is eliminated
Step (eliminate " in the distilled water for being dipped in pH 4.8 again, the pre- defluorinate processing of the ultrasonically treated 4h progress of 100W, every 1 during processing
Hour changes first water ").
Contrast groups 3：The method of be the same as Example 1, difference is：Modified bagasse powder is carried out after defluorinate processing, step (3)
In " the step of soaking 25min " in 80 DEG C of distilled water is omitted.
Contrast groups 4：The method of be the same as Example 1, difference is：Modified bagasse powder defluorinate process step is eliminated, i.e.,
Eliminate step (2).
(2) Oil repellent in euphausia superba powder prepared by each group is determined, shrimp med yield is calculated.
As a result as shown in Table 9：The maximum effect for modified bagasse powder is contributed euphausia superba powder defluorination effect, its
Secondary, the pre- defluorinate processing of distilled water and water immersion etc. also have important influence to defluorination effect.
Table 9
| Group | Shrimp med Oil repellent | Shrimp med yield |
| Experimental group | 0.4mg/kg | 92% |
| Contrast groups 1 | 3.4mg/kg | 90% |
| Contrast groups 2 | 9.5mg/kg | 91% |
| Contrast groups 3 | 6.1mg/kg | 93% |
| Contrast groups 4 | 46mg/kg | 90% |
The preparation and application of the hyperchromic feed of the fancy carp of embodiment 3
1st, the present invention is explored and verified by many experiments, draws a kind of special hyperchromic feed of fancy carp, is had very to fancy carp
Good color development hyperchromic effect, effect is notable, and attractant is good, can greatly improve resistance, survival rate.Specific implementation case is for example
Under：
Experimental example 1：A kind of special hyperchromic feed of fancy carp, is made up of the component of following mass percent：Euphausia superba powder
30%th, FSPC 6%, barley worm powder 4%, fly maggot powder 4%, Self- raising flour 10%, beer yeast powder 5%, starch
7%th, fairy shrimp 8%, spirulina 3%, soybean lecithin 3%, glycine betaine 3%, fish oil 2%, vitamin mixtures 2%, mineral member
It is plain mixture 3%, immunopotentiator 2.5%, lactic acid bacillus 2%, lilac 2.5%, Rhodopseudomonas palustris 1%, red
Green pepper powder 2%.
Experimental example 2：A kind of special hyperchromic feed of fancy carp, is made up of the component of following mass percent：Euphausia superba powder
40%th, FSPC 9%, barley worm powder 3%, fly maggot powder 3%, Self- raising flour 8%, beer yeast powder 5%, starch 7%,
Fairy shrimp 9%, spirulina 2%, soybean lecithin 2%, glycine betaine 2%, fish oil 1%, vitamin mixtures 1%, mineral element are mixed
Compound 2%, immunopotentiator 1%, lactic acid bacillus 1%, lilac 2%, Rhodopseudomonas palustris 0.5%, Paprika
1.5%.
Experimental example 3：A kind of special hyperchromic feed of fancy carp, is made up of the component of following mass percent：Euphausia superba powder
12%th, FSPC 10%, barley worm powder 4%, fly maggot powder 4%, Self- raising flour 10%, beer yeast powder 8%, starch
8%th, fairy shrimp 10%, spirulina 4%, soybean lecithin 3%, glycine betaine 3%, fish oil 2.5%, vitamin mixtures 2.5%, ore deposit
Thing element mixture 4.5%, immunopotentiator 5%, lactic acid bacillus 3%, lilac 3%, Rhodopseudomonas palustris 1%,
Paprika 2.5%.
Experimental example 4：A kind of special hyperchromic feed of fancy carp, is made up of the component of following mass percent：Euphausia superba powder
72%th, FSPC 2.4%, barley worm powder 1%, fly maggot powder 1%, Self- raising flour 5%, beer yeast powder 2%, starch
1%th, fairy shrimp 4%, spirulina 2%, soybean lecithin 1%, glycine betaine 2%, fish oil 1%, vitamin mixtures 1%, mineral member
It is plain mixture 1%, immunopotentiator 1%, lactic acid bacillus 0.5%, lilac 1%, Rhodopseudomonas palustris 0.1%, red
Green pepper powder 1%.
Experimental example 5：A kind of special hyperchromic feed of fancy carp, is made up of the component of following mass percent：Euphausia superba powder
5%th, FSPC 10%, barley worm powder 5%, fly maggot powder 5%, Self- raising flour 10%, beer yeast powder 5.5%, starch
10%th, fairy shrimp 10%, spirulina 4%, soybean lecithin 4%, glycine betaine 4%, fish oil 3%, vitamin mixtures 2.5%, ore deposit
It is thing element mixture 5%, immunopotentiator 5%, lactic acid bacillus 5%, lilac 3%, Rhodopseudomonas palustris 1%, red
Green pepper powder 3%.
Meanwhile, control group is set：Carp compound feed is viewed and admired as hyperchromic feed using purchased in market.The hyperchromic feed it is main into
It is divided into：Imported fish meal 15~20%, shrimp med 10~20%, corn protein powder 5~12%, flour 5~40%, Tubifex powder 3~
5%th, wheat embryo 2~3%, spirulina 3~6%, fish oil 4~6%, trace element 0.1~0.3%, vitamin 0.1~
0.5%th, calcium dihydrogen phosphate 2~3%, astaxanthin 0.3~0.5%.
2nd, in addition, to be also provided with contrast experiment as follows：
Comparative example 1：Substantially with experimental example 1, difference is euphausia superba powder being all changed to fish meal.
Comparative example 2：Substantially with experimental example 1, difference is all to change fly maggot powder into barley worm powder into.
Comparative example 3：Substantially with experimental example 1, difference is all to change barley worm powder into fly maggot powder.
Comparative example 4：Substantially with experimental example 1, difference is that the consumption for improving barley worm powder is 15%.
Comparative example 5：Substantially with experimental example 1, difference is that the consumption for improving fly maggot powder is 15%.
3rd, administering transgenic
(1) administering transgenic is carried out using the hyperchromic feed of above-mentioned each experimental example, comparative example.
Test place：Guangdong Jieyang plant of Guangdong Yuequn Ocean Biological Research Development Co., Ltd..
Experimental animal：The fancy carp fry of same batch hatching, cultivating is selected, is divided into 12 groups and is tested, every group of 3 repetitions,
It is each to repeat 1000 tails or so fry.Data statistics is averaged.
Feeding management is operated according to conventional cultivation.Fed once every timing in 8 hours in daily, every group each hyperchromic
Feedstuff feeding amount is 1.5g.
(2) result is counted
Observation statistics fry ingests situation, and record has fry to start the time ingested first, and fry more than half opens
Begin the time ingested.In order to reduce error as far as possible, three batch datas of three repetitions of each group are completed by three personal responsibilities respectively,
As a result take the mean.
The survival rate of fry after statistics is calculated 45.
Record starts the color development hyperchromic time.
(3) result is as shown in table 10.From experimental result, in the special hyperchromic feed of fancy carp of the present invention, each component
And its proportioning forms the organic whole of the coordinating and unifying, especially euphausia superba powder, fly maggot powder, barley worm powder, lactic acid gemma bar
Bacterium, Rhodopseudomonas palustris and Paprika show obvious synergistic function, and the hyperchromic effect of color development is notable, and can be notable
The attractant of feed is lifted, contributes to growing for fry.
Table 10
Influence of the Oil repellent of euphausia superba powder to fancy carp fry in the hyperchromic feed of embodiment 4
1st, based on the experimental example 1 in embodiment 3, using the euphausia superba powder of different Oil repellents as variable, preparing one is
Hyperchromic feed is arranged, is tested.Every group sets 3 repetitions, results averaged.
2nd, result is as shown in table 11
Table 11
Experimental result shows that euphausia superba powder Oil repellent height is not only there is fluorine in fish body the problem of high residue, and
And on fry grow and survival rate has obvious influence.When euphausia superba powder Oil repellent is higher, to the color development of fancy carp
It is hyperchromic also to have obvious influence.
Influence of the variety classes high protein insect powder of embodiment 5 to fancy carp fry
1st, based on the experimental example 1 in embodiment 3, difference is barley worm powder and fly maggot powder replacing with difference
The combination of insect albumen powder, prepares a series of hyperchromic feeds, is tested.Wherein, dried silkworm chrysalis meal, cricket powder, cicada pupa powder, bee pupa powder
It is that powder is made as raw material and obtains using the silkworm chrysalis propagated artificially, cricket, cicada pupa, bee pupa respectively.
Every group sets 3 repetitions, results averaged.Experimental design timing node is set to every 4 hour record once.
2nd, result is as shown in table 12
Table 12
Experimental result shown, variety classes high protein insect powder is hyperchromic to the color development of fancy carp to be had a certain impact.In addition, bright and beautiful
Carp more preference barley worm powder, fly maggot powder, cricket powder, bee pupa powder, optimal, ration size is combined as with barley worm powder and fly maggot powder
More preferably, to growing with more preferable facilitation effect, therefore, each factor, present invention selection barley worm powder and fly are considered
The combination of maggot powder.
Influence of the different types of microorganisms bacterium of embodiment 6 to fancy carp fry
1st, based on the experimental example 1 in embodiment 3, difference is lactic acid bacillus is all changed into table respectively
Strain shown in 13.Every group sets 3 repetitions, results averaged.
2nd, result is as shown in table 13
Table 13
As a result show, different microorganisms strain is hyperchromic to the color development of fancy carp to be had a certain impact；Meanwhile, to the food calling of fry
Property also have a certain impact, influence fry grows and survival rate, using lactic acid bacillus is optimal.
Influence of the use of the plant flowers of embodiment 7 to fancy carp fry
1st, based on the experimental example 1 in embodiment 3, difference is using different plant flowers as single argument, prepares one
The hyperchromic feed of series is tested.Every group sets 3 repetitions, results averaged, as shown in table 14.
Table 14
2nd, based on the experimental example 1 in embodiment 3, difference is using lilac consumption as single argument, prepares one
The hyperchromic feed of series is tested.Every group sets 3 repetitions, results averaged, as shown in Table 15.
Table 15
Above experimental result shows, different plants spend the attractant effect to juvenile fish seedling to have significant difference, so that aobvious
Write growing and survival rate for influence fancy carp fry.Wherein, lilac has significant attractant effect to fancy carp fry, and has agent
Measure dependence so as to fancy carp fry grow and survival rate has a certain impact.Meanwhile, color development of the plant flowers to fancy carp
It is hyperchromic also to have a certain impact, influence maximum with lilac, plum blossom and rose.Consider each factor, present invention selection fourth
Fragrant flower adds the hyperchromic feed of fancy carp.
Influence of the different pigments of embodiment 8 to fancy carp fry
Screened by substantial amounts of research, can promoting ornamental fish color development hyperchromic effect, significantly chromogenic plain bacterium has marsh red
Pseudomonad, monascus, rhodotorula glutinis etc., pigment have dragon fruit haematochrome, Red Pigment from Persimmon, Paprika, prodigiosin etc.；And
Both can show certain synergistic function by suitable use cooperatively.
This experiment is based on the experimental example 1 in embodiment 3, and difference is with different chromogenic plain bacterium and different pigments
For variable, prepare a series of hyperchromic feeds and tested.Every group sets 3 repetitions, results averaged, as shown in table 16.
Table 16
As a result show, under the premise of ensureing that attractant is more preferable, for promoting fancy carp color development hyperchromic effect to be most significantly
Rhodopseudomonas palustris and Paprika.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, should be all included within protection scope of the present invention.
Claims (10)
1. application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared.
2. application according to claim 1, it is characterised in that the Oil repellent of the euphausia superba powder is 0~1mg/kg.
3. application according to claim 2, it is characterised in that the euphausia superba powder is prepared by the following method：
S1. krill keeps 5~15min, then be dipped in pH 4.6~5.0 steaming after pretreatment in 80~85 DEG C of water
In distilled water, ultrasound carries out pre- defluorinate processing；
S2. defluorinate processing is carried out using modified bagasse powder：Ratio according to 15~20g/L, which adds modified bagasse powder, distills
In water, regulation pH is 4.5~5.0, and krill is immersed, and is reacted under ultrasound condition；
S3. the krill after step S2 processing is soaked into 20~30min in 75~85 DEG C of distilled water, then 40~45 DEG C
Drying to water content is 20~40%, is 10~15% then at 50~55 DEG C of drying to water content；
S4. the krill of drying is crushed to fineness for 120~220 mesh, it is 1~5% then to dry to water content, obtains low
Fluorine euphausia superba powder；
Wherein, the preparation method of modified bagasse powder is described in step S2：
S21. bagasse powder is pulverized and sieved to obtain by bagasse drying or after drying；
S22. bagasse powder is soaked after 6~7h in 20~30% sodium hydroxide solutions, washing, filtering；
S23. the product after S22 is handled is soaked in 1.5~2g/L liquor potassic permanganates, 50~80 DEG C of 1.5~3h of reaction；
S24. the product after S23 is handled is washed, filters, dried, and produces modified bagasse powder.
4. a kind of special hyperchromic feed of fancy carp, it is characterised in that be made up of the component of following mass percent：Euphausia superba powder 5
~72%, FSPC 1~20%, barley worm powder 1~5%, fly maggot powder 1~5%, Self- raising flour 5~24%, beer yeast powder 2
~10%, starch 1~10%, fairy shrimp 4~20%, spirulina 2~4%, soybean lecithin 1~4%, glycine betaine 2~4%, fish oil 1~
3%th, vitamin mixtures 1~2.5%, mineral element mixture 1~5%, immunopotentiator 1~5%, lactic acid bacillus 0.5~
5%th, lilac 1~3%, Rhodopseudomonas palustris 0.1~1%, Paprika 1~3%.
5. the special hyperchromic feed of fancy carp according to claim 4, it is characterised in that by the component system of following mass percent
Into：Euphausia superba powder 10~40%, FSPC 10~20%, barley worm powder 3~4%, fly maggot powder 3~4%, Self- raising flour 10
~20%, beer yeast powder 5~8%, starch 5~8%, fairy shrimp 8~15%, spirulina 2~4%, soybean lecithin 2~4%, glycine betaine
2~4%, fish oil 1~2.5%, vitamin mixtures 1~2.5%, mineral element mixture 2~5%, immunopotentiator 1~5%, breast
Sour bacillus 1~3%, lilac 2~3%, Rhodopseudomonas palustris 0.5~1%, Paprika 1.5~2.5%.
6. the special hyperchromic feed of fancy carp according to claim 4, it is characterised in that by the component system of following mass percent
Into：Euphausia superba powder 30%, FSPC 6%, barley worm powder 4%, fly maggot powder 4%, Self- raising flour 10%, beer yeast powder 5%,
Starch 7%, fairy shrimp 8%, spirulina 3%, soybean lecithin 3%, glycine betaine 3%, fish oil 2%, vitamin mixtures 2%, mineral element are mixed
Compound 3%, immunopotentiator 2.5%, lactic acid bacillus 2%, lilac 2.5%, Rhodopseudomonas palustris 1%, Paprika 2%.
7. according to any described special hyperchromic feed of fancy carp of claim 4~6, it is characterised in that the euphausia superba powder
Oil repellent is less than 1mg/kg.
8. according to any described special hyperchromic feed of fancy carp of claim 4~6, it is characterised in that the immunopotentiator is：
β -1,3 glucans and/or β -1,6 glucans.
9. according to any described special hyperchromic feed of fancy carp of claim 4~6, it is characterised in that the vitamin mixtures
Including：Vitamin A, vitamine D3, vitamin E, Vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12,
Nicotinic acid, calcium pantothenate, biotin, inositol, folic acid, vitamin C.
10. according to any described special hyperchromic feed of fancy carp of claim 4~6, it is characterised in that the mineral element mixing
Thing includes：Copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, cobalt chloride, sodium selenite, potassium dihydrogen phosphate, sodium dihydrogen phosphate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710296996.9A CN107183320A (en) | 2017-04-28 | 2017-04-28 | Application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710296996.9A CN107183320A (en) | 2017-04-28 | 2017-04-28 | Application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107183320A true CN107183320A (en) | 2017-09-22 |
Family
ID=59873055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710296996.9A Withdrawn CN107183320A (en) | 2017-04-28 | 2017-04-28 | Application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107183320A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108185117A (en) * | 2018-01-04 | 2018-06-22 | 广东越群海洋生物研究开发有限公司 | Hyperchromic feed of a kind of east star spot and preparation method thereof |
| CN110178971A (en) * | 2019-04-12 | 2019-08-30 | 华中农业大学 | Red white fancy carp is bred as mixed feed and its application |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690538A (en) * | 2009-09-24 | 2010-04-07 | 中国海洋大学 | Method for preparing low-fluorine Euphausia superba protein base materials |
| CN102559825A (en) * | 2012-01-18 | 2012-07-11 | 辽宁省大连海洋渔业集团公司 | Method for preparing antarctic krill low-fluorine hydrolysis polypeptide |
| CN103583931A (en) * | 2013-11-11 | 2014-02-19 | 山东师范大学 | Ultrasonic defluorination method for whole euphausia superba |
| CN104996784A (en) * | 2015-06-26 | 2015-10-28 | 浙江海洋学院 | Fish feed using euphausia superba as the main raw material and a preparation method thereof |
| CN105231088A (en) * | 2015-08-31 | 2016-01-13 | 青岛海之源智能技术有限公司 | Compound feed capable of enabling color of cryprinus carpiod to be enhanced and preparation method of compound feed |
| CN105419936A (en) * | 2015-11-04 | 2016-03-23 | 大连工业大学 | Preparation method of low fluorine content Euphausia superba oil |
| CN105664842A (en) * | 2016-04-07 | 2016-06-15 | 景德镇陶瓷学院 | Calcium-based adsorbent for defluorination and application thereof |
-
2017
- 2017-04-28 CN CN201710296996.9A patent/CN107183320A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690538A (en) * | 2009-09-24 | 2010-04-07 | 中国海洋大学 | Method for preparing low-fluorine Euphausia superba protein base materials |
| CN102559825A (en) * | 2012-01-18 | 2012-07-11 | 辽宁省大连海洋渔业集团公司 | Method for preparing antarctic krill low-fluorine hydrolysis polypeptide |
| CN103583931A (en) * | 2013-11-11 | 2014-02-19 | 山东师范大学 | Ultrasonic defluorination method for whole euphausia superba |
| CN104996784A (en) * | 2015-06-26 | 2015-10-28 | 浙江海洋学院 | Fish feed using euphausia superba as the main raw material and a preparation method thereof |
| CN105231088A (en) * | 2015-08-31 | 2016-01-13 | 青岛海之源智能技术有限公司 | Compound feed capable of enabling color of cryprinus carpiod to be enhanced and preparation method of compound feed |
| CN105419936A (en) * | 2015-11-04 | 2016-03-23 | 大连工业大学 | Preparation method of low fluorine content Euphausia superba oil |
| CN105664842A (en) * | 2016-04-07 | 2016-06-15 | 景德镇陶瓷学院 | Calcium-based adsorbent for defluorination and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 李芬芳: ""KMnO4改性甘蔗渣对水中Pb2+的吸附"", 《工业水处理》 * |
| 牛显春: ""氯化锌与高锰酸钾混合改性甘蔗渣重金属吸附剂的研究"", 《化学工程师》 * |
| 田野等: ""纤维素基吸附剂-绿色、经济的水处理材料"", 《环境化学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108185117A (en) * | 2018-01-04 | 2018-06-22 | 广东越群海洋生物研究开发有限公司 | Hyperchromic feed of a kind of east star spot and preparation method thereof |
| CN108185117B (en) * | 2018-01-04 | 2021-06-01 | 广东越群海洋生物研究开发有限公司 | Dongxiang spot color-increasing feed and preparation method thereof |
| CN110178971A (en) * | 2019-04-12 | 2019-08-30 | 华中农业大学 | Red white fancy carp is bred as mixed feed and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103719004B (en) | A kind of efficient pond rearing method of loach large size seedling seed | |
| CN103478081B (en) | Indoor high-density cultivation method of artemia | |
| CN104171583B (en) | A kind of Procambarus clarkii feedstuff and preparation method thereof | |
| CN103535555B (en) | Feed for tiger frog tadpoles and bullfrog tadpoles and preparation method of feed | |
| CN101779748B (en) | Activity enhancer for shrimps, crabs and fish in aquaculture and preparation method thereof | |
| CN103404472B (en) | Industrialized ecological cultivation method of ribbonfish | |
| CN106376778A (en) | Turbot fry starter feed formula | |
| CN105123563A (en) | Mixed culture method for penaeus vannamei and macrobrachium rosenbergii | |
| CN102150754A (en) | Organic Se-rich shrimp and breeding method thereof | |
| CN106798196A (en) | A kind of low fish meal jewfish expanding compound feed of recycling glasswort | |
| CN106417122B (en) | A kind of breeding method improving high-quality freshwater shrimp seed yield | |
| CN107529411A (en) | A kind of mixed feed of Scylla paramamosain soft crab and preparation method thereof | |
| CN107156426A (en) | Application of the euphausia superba powder in terms of the hyperchromic breeding feed of Salmons is prepared | |
| CN107183320A (en) | Application of the euphausia superba powder in terms of the hyperchromic feed of fancy carp is prepared | |
| CN103549185B (en) | Artificial microparticle feed for clam worm larva and preparation method thereof | |
| CN107410757A (en) | The preparation method of the composite fluid bait of coral artificial farming | |
| CN100398001C (en) | Bait with enzymolysis being needed before use for shellfish | |
| CN108633788A (en) | A kind of Pangasius sutchi low salinity stocking method | |
| CN108496856A (en) | A kind of cultural method of grass carp | |
| CN107646765A (en) | The cultural method of cray | |
| CN107156427A (en) | Application of the euphausia superba powder in terms of the hyperchromic feed of Poecilia is prepared | |
| CN108117436A (en) | A kind of zinc-rich nutrient liquid and its preparation method and application | |
| CN107252007A (en) | Application of the euphausia superba powder in terms of the hyperchromic feed of golden dragonfish is prepared | |
| CN107198058A (en) | Application of the euphausia superba powder in terms of Pseudorabora parva opening material is prepared | |
| CN107156441A (en) | Application of the euphausia superba powder in terms of blood parrot color lifting is prepared |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170922 |