CN107182949A - A kind of test device and its method of testing of insect repellent behavior - Google Patents

A kind of test device and its method of testing of insect repellent behavior Download PDF

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Publication number
CN107182949A
CN107182949A CN201710446539.3A CN201710446539A CN107182949A CN 107182949 A CN107182949 A CN 107182949A CN 201710446539 A CN201710446539 A CN 201710446539A CN 107182949 A CN107182949 A CN 107182949A
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China
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casing
insect
interface channel
test device
insect repellent
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CN201710446539.3A
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陈俊铭
肖宽诚
张秋金
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Fujian Normal University
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Fujian Normal University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Catching Or Destruction (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of test device and its method of testing of insect repellent behavior, test device includes the first casing, second casing, 3rd casing, first interface channel and the second interface channel, the two ends of first interface channel are respectively communicated with first casing and the second casing, the two ends of second interface channel are respectively communicated with second casing and the 3rd casing, first interface channel is respectively equipped with the first infrared sensor and the first counter close to the exit of the first casing, first infrared sensor is electrically connected with first counter, second interface channel is respectively equipped with the second infrared sensor and the second counter close to the exit of the 3rd casing, second infrared sensor is electrically connected with second counter.When carrying out the test of insect repellent behavior using the test device, it can reliably reflect that insect walks quickly and keeps away behavior to medicine.

Description

A kind of test device and its method of testing of insect repellent behavior
Technical field
The present invention relates to test device and its test side of technical field of measurement and test, more particularly to a kind of insect repellent behavior Method.
Background technology
Cockroach belongs to Insecta, and Blattaria is the time-honored insect of a class, and will of the original cockroach about before 400,000,000 years is stayed Come across in discipline on the earth.At present with human lives it is in close relations mainly have American cockroach, Groton bug, the small Lian of black chest, Australia The big Lian in the continent and big Lian of Japan, and it is most commonly that American cockroach and Groton bug in China.
Cockroach is more than allowing people to seem nausea, it is often more important that it carries various bacteria and parasite egg, is a lot The disseminator of human diseases.Cockroach has been demonstrated to carry about 40 kinds of mushrooms caused a disease to vertebrate, such as infects the leprosy of leprosy Mycobacterium, the yersinia pestis for infecting bubonic plague etc., cockroach can also carry roundworm, Ancylostoma duodenale, beef tapeworm, pinworm A variety of worm egg such as worm, whipworm.
Cockroach is more than spreading disease, and cockroach also often stings Shiqi his article, such as cotton wool product, leather and fur products, paper, book Nationality etc..Therefore cockroach not just a kind of sanitary insect pest, while being also archives insect and textile insect.In addition, cockroach is also Usually in the electrical equipment such as drilling computer, refrigerator, communication apparatus, electrical short-circuit is caused to burn.
The method one that the current mankind tackle cockroach is cleaning ambient, breaks off the food source of cockroach, this is in real life It is difficult to accomplish completely;Two be to kill cockroach using insecticide.Cockroach is a kind of fertility and survival ability all unusual strong elder brothers Worm, insecticide never really solved problem, also created the pollution of environment.In some places such as kitchen, food trough The place such as storehouse can have safety problem using insecticide, and for Buddhism personage, the theory of ahimsa also prevents them to make Cockroach is tackled with insecticide.Therefore the research to cockroach repellant has critically important practical significance.
Cockroach repellent agent is studied, first have to research cockroach walks quickly and keeps away behavior.The research of cockroach repellent behavior is usual at present Using filter paper method of testing, small container method of testing and labyrinth method of testing etc..Patent No. CN201120218624.2 Chinese patent A kind of insect olfaction performance testing device is disclosed, but there are various problems in test process, such as odoring substance is detained, thing Matter itself is volatile not to cause test result untrue on an equal basis.And the common ground of these existing methods is:Pass through dispenser Statistics cockroach assesses repellent effect having medicine direction with the distribution number without medicine direction in the short time afterwards, uses these methods Have the shortcomings that some can not overcome when studying the repellent behavior of cockroach, it is impossible to the true shadow for reflecting medicine to cockroach activity Ring.Cockroach activity, which can be impacted, for example when someone aside observes causes result of study inaccurate;Cockroach is a kind of night Behavior pattern under row insect, illumination from it is dark in behavior pattern be different.Therefore, these test dresses used at present Put and its method for testing degree of reliability is not high, it is difficult to truly reflect anti-repelling properties of the cockroach to medicine.
The content of the invention
The technical problems to be solved by the invention are:A kind of test device and its method of testing of insect repellent behavior, energy Reliably reflect that insect walks quickly and keeps away behavior to medicine.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is:
A kind of test device of insect repellent behavior, including the first casing, the second casing, the 3rd casing, the first connection are logical Road and the second interface channel, the two ends of first interface channel are respectively communicated with first casing and the second casing, described The two ends of two interface channels are respectively communicated with second casing and the 3rd casing, and first interface channel is close to the first casing Exit is respectively equipped with the first infrared sensor and the first counter, first infrared sensor and first counter electricity Connection, second interface channel is respectively equipped with the second infrared sensor and the second counter close to the exit of the 3rd casing, Second infrared sensor is electrically connected with second counter.
Another technical scheme that the present invention is used for:
A kind of method of testing of insect repellent behavior, is put into the food and water of preset weight, described in the first casing The food, water and medicine of preset weight are put into 3rd casing, the insect of predetermined number is put into the second casing;Interval is pre- If the time weighs the weight of remaining food in the first casing and the 3rd casing respectively, the consumption for obtaining food is calculated;According to The consumption of the food calculate obtain insect walk quickly and keep away rate.
The beneficial effects of the present invention are:The second casing is set between the first casing and the 3rd casing, elder brother can be increased The scope of activities of worm, when holding medicine in the 3rd casing, the second casing can also not allow in the 3rd casing as buffer zone Medicine enter the first casing so as to influenceing experimental result;And in the exit of interface channel, infrared sensor and counting are set Device, the number of times progress accurate counting of insect can be come in and gone out the first casing and the 3rd casing;The insect repellent rate of the present invention program Method of testing it is not only workable, be easily achieved, and experimental result is really reliable, and the cockroach that is particularly suitable for use in walks quickly and keeps away behavior Test analysis.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the test device of the insect repellent behavior of the embodiment of the present invention one;
Fig. 2 is the profile of the test device of the insect repellent behavior of the embodiment of the present invention one.
Label declaration:
1st, the first casing;2nd, the second casing;3rd, the 3rd casing;4th, the first interface channel;5th, the second interface channel;6th, it is infrared Transmitter;7th, infrared remote receiver;8th, pore structure.
Embodiment
To describe technology contents, the objects and the effects of the present invention in detail, below in conjunction with embodiment and coordinate attached Figure is explained.
The design of most critical of the present invention is:Second casing is set between the first casing and the 3rd casing, and even Connect and infrared sensor and counter are set on road.
It refer to Fig. 1 and Fig. 2, a kind of test device of insect repellent behavior, including the first casing, the second casing, Three casings, the first interface channel and the second interface channel, the two ends of first interface channel are respectively communicated with first casing With the second casing, the two ends of second interface channel are respectively communicated with second casing and the 3rd casing, first connection Passage is respectively equipped with the first infrared sensor and the first counter, first infrared sensor close to the exit of the first casing Electrically connected with first counter, second interface channel is respectively equipped with the second infrared biography close to the exit of the 3rd casing Sensor and the second counter, second infrared sensor are electrically connected with second counter.
It was found from foregoing description, the beneficial effects of the present invention are:Second is set between the first casing and the 3rd casing Casing, can increase the scope of activities of insect, when placing different medicines respectively in the first casing and the 3rd casing, the second case Body can also be as buffer zone, and the medicine not allowed in the first casing or the 3rd casing enters another casing to influence experiment As a result, the shapes and sizes of the first casing, the second casing and the 3rd casing can be configured as needed;In interface channel Exit sets infrared sensor and counter, and the number of times of insect the first casing of discrepancy and the 3rd casing accurately can be counted Number, can further be verified with number of pass times to the behavior of walking quickly and keeping away of insect;Interface channel can be square or circle, with Insect can be by being defined.
Further, it is respectively equipped with dismountable door on first casing and the 3rd casing.
Seen from the above description, setting dismountable door on the first casing and the 3rd casing can facilitate to the first case Food for being tested etc. is put into body and the 3rd casing, the position of door and size can be configured as needed.
Further, pore structure is respectively equipped with first casing and the 3rd casing, the pore structure is provided with gauze.
Seen from the above description, apertured structure and be covered with gauze on casing, available for ventilating, and prevent insect from Climbed out of in pore structure, the sizing grid of gauze can be configured as needed, pore structure can not be set by being defined by insect Ventilation can make test environment be more nearly living environment so that test result is more accurate.
Further, second casing is provided with pore structure.
Seen from the above description, set pore structure to be ventilated on the second casing, can prevent from being placed with the case of medicine Drug molecule in body enters in the casing without holding medicine, makes test result relatively reliable.
Further, adsorbent equipment is provided with second casing.
Seen from the above description, adsorbent equipment can be used for the drug molecule absorbed into the second casing.
Further, first casing and the 3rd casing are symmetrical arranged relative to second casing.
Further, first interface channel is removably connected under first casing and the second casing respectively Portion, second interface channel removably connects the bottom of second casing and the 3rd casing respectively.
Seen from the above description, it is detachably connected between interface channel and casing, convenient storage and transport, and connect Passage connects the bottom of casing, facilitates insect to enter interface channel.
A kind of method of testing of insect repellent behavior, is put into the food and water of preset weight in first casing, The food, water and medicine of preset weight are put into 3rd casing, the insect of predetermined number is put into the second casing;Between The weight of remaining food in the first casing and the 3rd casing is weighed respectively every preset time, calculates the consumption for obtaining food; Calculated according to the consumption of the food obtain insect walk quickly and keep away rate.
Seen from the above description, the rate of walking quickly and keeping away is calculated by the consumption of the first casing of calculating and the food of the 3rd casing, Method of testing is simple, easy.
Further, calculated according to formula (W1-W3)/(W1+W3) × 100% obtain insect walk quickly and keep away rate, wherein, W1 is The consumption of food in first casing, W3 be the 3rd casing in food consumption.
Further, include the number of times of record insect the first casing of discrepancy and the 3rd casing, calculated according to the number of times Obtain insect walks quickly and keeps away rate.
Seen from the above description, what the number of times for passing in and out casing according to insect can further assess insect walks quickly and keeps away behavior.
Embodiment one
Fig. 1 and Fig. 2 is refer to, embodiments of the invention one are:
A kind of test device of insect repellent behavior, can reliably reflect that insect is walked quickly and kept away to medicine using the test device Behavior, is particularly suitable for use in and the research of behavior is walked quickly and kept away to cockroach.
As shown in figure 1, the test device includes the first casing 1, the second casing 2, the 3rd casing 3, the first interface channel 4 With the second interface channel 5, the two ends of first interface channel 4 are respectively communicated with the casing 2 of the first casing 1 and second, described The two ends of second interface channel 5 are respectively communicated with the casing 3 of the second casing 2 and the 3rd, it is preferred that first interface channel 4 The bottom of the casing 2 of the first casing 1 and second is removably connected respectively, and the difference of the second interface channel 5 is removably The bottom of the casing 3 of the second casing 2 and the 3rd is connected, the casing 3 of the first casing 1 and the 3rd is relative to second casing 2 are symmetrical arranged.In the present embodiment, the first casing 1, the second casing 2 and the 3rd casing 3 are square, and three casings is big It is small consistent, it is of course also possible to be designed as other shapes as needed, such as cylindrical or spherical, three casings it is big It is small to may be designed in difference.First casing 1, the second casing 2 and the 3rd casing 3 can be by transparent, translucent or opaque Material be made, walk quickly and keep away behavior with study the insect under different illumination intensity.First interface channel 4 and second is connected Passage 5 can be round tubular structure, naturally it is also possible to be square tubular structure, the first interface channel 4 and the second interface channel 5 Length can be configured as needed, for example can be designed as 1m or so.First interface channel 4 and the second interface channel 5 It can also be made up of transparent, translucent or opaque material.
In the present embodiment, dismountable door is respectively equipped with the casing 3 of the first casing 1 and the 3rd, door Shapes and sizes can be configured as needed, for example, can be designed as circular or square, size can be passed through with the hand of people It is defined.Pore structure 8 is further respectively had on the first casing 1 and the 3rd casing 3, the pore structure 8 is provided with gauze, the net of gauze Hole size can be selected as needed.Pore structure 8 can be arranged at least one side of casing, can also be arranged on The top surface of casing, it is preferred that pore structure 8 is arranged on two relative sides of casing, so as to air formation convection current.Described second It also is provided with being also covered with gauze in pore structure 8, pore structure 8 on casing 2, multiple pore structures 8, institute can be set on the second casing 2 State and adsorbent equipment is additionally provided with the second casing 2, adsorbent equipment is built with adsorbents such as activated carbons.
As shown in Fig. 2 in the present embodiment, first interface channel 4 is respectively equipped with close to the exit of the first casing 1 One infrared sensor and the first counter, first infrared sensor are electrically connected with first counter, it is preferred that by One infrared sensor is arranged on the first interface channel 4 close to the exit of the first casing 1.Second interface channel 5 is close to The exit of three casings 3 is respectively equipped with the second infrared sensor and the second counter, second infrared sensor and described the Two counters are electrically connected, it is preferred that the second infrared sensor is arranged on into the second interface channel 5 close to the outlet of the 3rd casing 3 Place.First infrared sensor and the second infrared sensor include infrared transmitter 6 and infrared remote receiver 7, described infrared Transmitter 6 and infrared remote receiver 7 are oppositely arranged.
The present embodiment further relates to a kind of method of testing of insect repellent behavior, mainly comprises the following steps:
1st, the food and water of preset weight are put into first casing 1, default weight is put into the 3rd casing 3 Food, water and the medicine of amount, the first casing 1 and food and the equal equivalent of water in the 3rd casing 3, the difference is that the 3rd casing 3 In be put into medicine, the medicine can be slow-release medicine.The insect of predetermined number is put into the second casing 2.
2nd, interval preset time weighs the weight of remaining food in the first casing 1 and the 3rd casing 3 respectively, and calculating is obtained The consumption of food;Calculated according to the consumption of the food obtain insect walk quickly and keep away rate.That is, according to formula (W1-W3)/(W1+ W3) × 100% calculate obtain insect walk quickly and keep away rate, wherein, W1 is the consumption of the food in the first casing 1, and W3 is the 3rd case The consumption of food in body 3.
3rd, by infrared sensor and the number of times of counter records insect the first casing 1 of discrepancy and the 3rd casing 3, according to institute State number of times calculate obtain insect walk quickly and keep away rate.
Embodiment two
Embodiments of the invention two are the concrete application scene of embodiment one, and specially a kind of cockroach walks quickly and keeps away the test of behavior Method.
First, Japan pepper essential oil sustained release agent is prepared.Four groups of sustained release agents are prepared, each group is respectively first distilled 1g agar and 30mL Water is poured into beaker and heated, and makes agar all dissolvings.0.2mL absolute ethyl alcohols are added with liquid-transfering gun is each in EP pipes, then are added Enter 0.05mL Tween 80s, Japan pepper essential oil 0.1mL, 0.2mL, 0.3mL and 0.4mL are separately added into after being mixed, EP pipes are put in Vibrated on vibrating machine, mix solution.When agar solution is cooled to room temperature, the essential oil solution in EP pipes is added in beaker Agar solution in, agitate by adding agar solution, make to be poured slowly into 10mL culture dishes after it is well mixed, to agar again Solidification, with film seal to avoid essential oil from volatilizing.
During test, by Nature enemy 20 American cockroaches of 12 hours (or 100 Groton bugs) are put into second in advance In casing, it is put into the first casing in 1g foods and 50mL water (being attached separately in small culture dish), the 3rd casing and is also placed in 1g foods Be free of in thing and 50mL water (being attached separately in small culture dish), and the Japan pepper essential oil sustained release agent for testing, control group sustained release agent Japan pepper essential oil.Test under field conditions (factors) (25~38 DEG C of temperature, humidity 70~85%) is carried out, leftover amount of weighing daily, Terminate when control group food consumption total amount is equal to about half of the total quantity of goods produced in both sides in experiment, the present embodiment, be at the 7th day Terminate experiment.First casing and the are passed in and out with the food consumption quantity situation in the first casing in the time and the 3rd casing and cockroach The number of times of three casings assesses the drive cockroach effect of medicine.Calculated according to formula (W1-W3)/(W1+W3) × 100% and obtain insect Walk quickly and keep away rate, the cockroach number of times of statistics the first casing of turnover and the 3rd casing daily is walked quickly and kept away the complementary of behavior as cockroach and commented Sentence index.
The experimental result of the present embodiment is as follows:
Various dose Japan pepper essential oil is shown in Tables 1 and 2 to American cockroach and Groton bug repellent effect, and experimental data is 3 The average value of secondary experimental data.
Scattering ratio (seven day) of the Japan pepper essential oil agar sustained release agent of table 1 to American cockroach
Scattering ratio (seven day) of the Japan pepper essential oil agar sustained release agent of table 2 to Groton bug
It will be evident that Japan pepper essential oil has significantly repellent effect to American cockroach and Groton bug from Tables 1 and 2, American cockroach is more sensitive to Japan pepper essential oil.
Influence (number of times that enters casing) of the Japan pepper essential oil agar sustained release agent of table 3 to American cockroach activity
Influence (number of times that enters casing) of the Japan pepper essential oil agar sustained release agent of table 4 to Groton bug activity
From table 3 and table 4 as can be seen that Japan pepper essential oil all has preferable repellent to imitate to American cockroach or Groton bug Really.In the case of no medicine, cockroach passes in and out the first casing and the frequency of the 3rd casing is essentially identical.Cockroach is to Japan pepper essential oil There are obvious anti-repelling properties:In the case where early stage food is sufficient, cockroach evident from the anti-repelling properties gone out to Japan pepper essential oil, and With concentration dependent.With the food consumption in the first casing, the frequency that cockroach passes in and out the 3rd casing just gradually increases, explanation Japan pepper essential oil has avoidance effect to cockroach.
In summary, the test device and its method of testing for a kind of insect repellent behavior that the present invention is provided, test device Simple in construction, it is easy to make, when carrying out the test of insect repellent behavior using the test device, obtained test result is trueer Real reliable, be particularly suitable for use in the test for walking quickly and keeping away behavior to cockroach.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair The equivalents that bright specification and accompanying drawing content are made, or the technical field of correlation is directly or indirectly used in, similarly include In the scope of patent protection of the present invention.

Claims (10)

1. a kind of test device of insect repellent behavior, it is characterised in that including the first casing, the second casing, the 3rd casing, A connection channel and the second interface channel, the two ends of first interface channel are respectively communicated with first casing and the second case Body, the two ends of second interface channel are respectively communicated with second casing and the 3rd casing, and first interface channel is close The exit of first casing is respectively equipped with the first infrared sensor and the first counter, first infrared sensor and described the One counter is electrically connected, and second interface channel is respectively equipped with the second infrared sensor and the close to the exit of the 3rd casing Two counters, second infrared sensor is electrically connected with second counter.
2. the test device of insect repellent behavior according to claim 1, it is characterised in that first casing and the 3rd Dismountable door is respectively equipped with casing.
3. the test device of insect repellent behavior according to claim 1, it is characterised in that first casing and the 3rd Pore structure is respectively equipped with casing, the pore structure is provided with gauze.
4. the test device of insect repellent behavior according to claim 1, it is characterised in that second casing is provided with Pore structure.
5. the test device of insect repellent behavior according to claim 1, it is characterised in that be provided with second casing Adsorbent equipment.
6. the test device of insect repellent behavior according to claim 1, it is characterised in that first casing and the 3rd Casing is symmetrical arranged relative to second casing.
7. the test device of insect repellent behavior according to claim 1, it is characterised in that first interface channel point The bottom of first casing and the second casing is not connected removably, second interface channel removably connects institute respectively State the bottom of the second casing and the 3rd casing.
8. a kind of method of testing of the insect repellent behavior of the test device based on described in claim 1-7 any one, it is special Levy and be, the food and water of preset weight are put into first casing, preset weight is put into the 3rd casing Food, water and medicine, are put into the insect of predetermined number in the second casing;It is spaced after preset time, the first case is weighed respectively The weight of remaining food in body and the 3rd casing, calculates the consumption for obtaining food;Calculated according to the consumption of the food Obtain insect walks quickly and keeps away rate.
9. the method for testing of insect repellent behavior according to claim 8, it is characterised in that according to formula (W1-W3)/ (W1+W3) × 100% calculate obtain insect walk quickly and keep away rate, wherein, W1 is the consumption of the food in the first casing, and W3 is the 3rd The consumption of food in casing.
10. the method for testing of insect repellent behavior according to claim 8, it is characterised in that also go out including record insect Enter the number of times of the first casing and the 3rd casing, calculated according to the number of times obtain insect walk quickly and keep away rate.
CN201710446539.3A 2017-06-14 2017-06-14 A kind of test device and its method of testing of insect repellent behavior Pending CN107182949A (en)

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CN110521675A (en) * 2019-09-18 2019-12-03 清华大学 Y type labyrinth for the experiment of honeybee praxiology research
CN110672827A (en) * 2019-10-21 2020-01-10 重庆师范大学 Pesticide effect experimental device and method for indoor space insect repellents
CN111937813A (en) * 2020-07-13 2020-11-17 河南工业大学 Insect behavior testing arrangement that keeps away
TWI754882B (en) * 2020-01-08 2022-02-11 國立高雄大學 An acrawl pest repellent test device
CN114217016A (en) * 2021-11-29 2022-03-22 佛山欧神诺陶瓷有限公司 Device and method for testing cockroach prevention performance of ceramic tile

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Publication number Priority date Publication date Assignee Title
CN110521675A (en) * 2019-09-18 2019-12-03 清华大学 Y type labyrinth for the experiment of honeybee praxiology research
CN110672827A (en) * 2019-10-21 2020-01-10 重庆师范大学 Pesticide effect experimental device and method for indoor space insect repellents
TWI754882B (en) * 2020-01-08 2022-02-11 國立高雄大學 An acrawl pest repellent test device
CN111937813A (en) * 2020-07-13 2020-11-17 河南工业大学 Insect behavior testing arrangement that keeps away
CN114217016A (en) * 2021-11-29 2022-03-22 佛山欧神诺陶瓷有限公司 Device and method for testing cockroach prevention performance of ceramic tile

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Application publication date: 20170922