CN107182775A - The sterile line rapid transfer method of Shan 5201 - Google Patents
The sterile line rapid transfer method of Shan 5201 Download PDFInfo
- Publication number
- CN107182775A CN107182775A CN201710450100.8A CN201710450100A CN107182775A CN 107182775 A CN107182775 A CN 107182775A CN 201710450100 A CN201710450100 A CN 201710450100A CN 107182775 A CN107182775 A CN 107182775A
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- China
- Prior art keywords
- sterile
- transformation
- shan
- sterile line
- rapid transfer
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the sterile line rapid transfer method in Shan 5201, the selection comprises the following steps：Self incompatible line 5201, is implanted into effect preferably using purple green stem vegetable sterile source, can reach 100% sterile rate and 100% sterile degree；During the sterile line transformation of Shan 5201, by seed selection normal individual plant continuation transformation of similar and flower bud development to maintainer from 2nd generation segregating population, the purpose of rapid transfer can be so obtained.Meanwhile, the high individual plant of seed selection index of affinity carries out sterile line transformation, can greatly reduce the cost of breeding parent, improve hybrid seed yield.
Description
Technical field
The present invention relates to agricultural breeding field, and in particular to a kind of sterile line rapid transfer method in Shan 5201.
Background technology
With system, (the recessive male of single-gene is or not the male sterility two that Chinese scholar found and initiated in early 1970s
Educate) once once it had been bred as kind and in production using (such as Chinese cabbage 156, the fast dish in Shenyang, Zhengzhou early black leaf), but due to system
50% fertile plant need to be repeatedly pulled out when planting, it is time-consuming to take a lot of work, and also often hybrid seed yield is relatively low, so as to limit the sterile type
Development and utilization.Academy of agricultural sciences of Shenyang City Zhang Shufang cultivates nucleus gene interactive effect male sterile line first and improved crossing kind is " fast
Dish one " etc. is widely applied.Such male sterile line, sterile degree and sterile rate can reach 100%, can make
High-quality hybrid, seed collecting amount is also higher, and it has the disadvantage that, equivalent to triple hybrid, hybrid regularity is under some influence, is additionally present of
The problem of transformation is more difficult.Agricultural University Of Shenyang to instruct, is designed with " multiple allele heredity hypothesis " and implements new core
Genic male sterility transformation scheme.Chinese cabbage genetic male sterile line with 100% sterile plant rate, its maintainer can only make
The sterility of sterile plant temporarily holds a generation, it is thus impossible to transformation be carried out with general saturation backcross method, so as to limit this not
Educate the application in source.It is male that vegetables institute of academy of agricultural sciences of Shaanxi Province Ke Gui orchids (1992) cultivate Chinese cabbage cabbage type rape kytoplasm first
Property sterile line, has been bred as the miscellaneous 13 grade popularization and application in production of cenospecies.Easily, parent does not degenerate for such male sterility transformation,
But category temperature sensitive type infertility recovers fertility in temperature change.So, male sterile line of cabbage transfer technology is badly in need of further complete
Kind and research.
The content of the invention
To solve the above problems, the invention provides a kind of sterile line rapid transfer method in Shan 5201.
To achieve the above object, the technical scheme taken of the present invention is：
The sterile line rapid transfer method in Shan 5201, comprises the following steps：
Using purple green stem vegetable sterile source to Shan 5201 carry out transformation, during transformation, from 2nd generation segregating population seed selection with
Maintainer is similar and the normal individual plant of flower bud development continues transformation.
The invention has the advantages that：
The sterile line of Shan 5201 obtained by the present invention, the implantation using purple green stem vegetable sterile source to the Chinese cabbage inbred lines of Shan 5201
Effect preferably, can reach 100% sterile rate and 100% sterile degree；During the sterile line transformation of Shan 5201, by from
Seed selection is similar to maintainer in 2nd generation segregating population and the normal individual plant of flower bud development continues transformation, can so obtain quick
The purpose of transformation.Meanwhile, the high individual plant of seed selection index of affinity carries out sterile line transformation, can greatly reduction breeding parent into
This, improves hybrid seed yield.
Brief description of the drawings
Fig. 1 is the flow chart of the embodiment of the present invention.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Embodiment
1st, transformation is carried out to the Chinese cabbage inbred lines of Shan 5201 with three kinds of sterile sources
The source of three kinds of sterile sources and sterile mechanism and performance：
(1) Chinese cabbage sterile source：Belong to Chinese cabbage cabbage type rape cytoplasmic male sterilty source.Temperature sensitive type, low temperature is fertile, high
Warm infertility；There is dead flower bud phenomenon.
(2) tender flower stalk sterile source：It is the nucleo-cytoplasmic interreaction sterile source that applicant has found and purified from tender flower stalk original variety.With white
Dish self-mating system transformation, can have two kinds of restorer and maintainer.When material is fully purified, maintainer can be obtained
100% sterile rate and 100% sterile degree, dead flower bud phenomenon are lighter.
(3) purple green stem vegetable sterile source：Found from the purple green stem vegetable kind of introduction, belong to cytoplasmic sterility, sterile rate and not
Degree of educating is 100%.Between green stem vegetable kind during transformation, dead flower bud phenomenon is serious.
2nd, optimal sterile source is selected
(1) three kind of sterile source transformation effect (being shown in Table 1)
Transformation effect of the 1 three kinds of sterile sources of table to suitable Inbred Lines
As it can be seen from table 1 after three kinds of sterile source transformations, wherein after Chinese cabbage sterile source and tender flower stalk sterile source transformation,
Performance half is sterile, and purple green stem vegetable sterile source shows complete sterility；In addition, Chinese cabbage sterile source and purple green stem vegetable sterile source have dead flower bud
Phenomenon, a kind of sedge sterile source is without dead flower bud phenomenon.It is observed that half sterile phenomenon has certain correlation with temperature, performance can during low temperature
Educate, after temperature gradually rises, start to show complete sterility.
As can be seen from the above results, three kinds of sterile sources are larger to the implantable difference on effect of the self-mating system of Shan 5201.Wherein
Purple green stem vegetable sterile source is best to the implantation effect of Chinese cabbage inbred lines, can reach 100% sterile rate and 100% infertility
Degree.
During the sterile line transformation of Shan 5201, seed selection is similar to maintainer from 2nd generation segregating population and flower bud development
Normal individual plant continues transformation.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (1)
1. the sterile line rapid transfer method in Shan 5201, it is characterised in that using Shan 5201 of the purple green stem vegetable sterile source to gained
Transformation is carried out, during transformation, seed selection is similar to maintainer from 2nd generation segregating population and the normal individual plant of flower bud development continues
Transformation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710450100.8A CN107182775A (en) | 2017-06-07 | 2017-06-07 | The sterile line rapid transfer method of Shan 5201 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710450100.8A CN107182775A (en) | 2017-06-07 | 2017-06-07 | The sterile line rapid transfer method of Shan 5201 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107182775A true CN107182775A (en) | 2017-09-22 |
Family
ID=59878016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710450100.8A Pending CN107182775A (en) | 2017-06-07 | 2017-06-07 | The sterile line rapid transfer method of Shan 5201 |
Country Status (1)
| Country | Link |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1144036A (en) * | 1996-05-27 | 1997-03-05 | 河南省新乡市农业科学研究所 | Crossbreeding method for Chinese cabbage |
| US20080098495A1 (en) * | 2006-09-13 | 2008-04-24 | Syngenta Participations Ag | Novel rucola plants with cytoplasmic male sterility (cms) |
| CN101480164B (en) * | 2009-02-18 | 2011-03-23 | 西北农林科技大学 | Method for breeding novel germplasm of Chinese cabbage with purple core |
-
2017
- 2017-06-07 CN CN201710450100.8A patent/CN107182775A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1144036A (en) * | 1996-05-27 | 1997-03-05 | 河南省新乡市农业科学研究所 | Crossbreeding method for Chinese cabbage |
| US20080098495A1 (en) * | 2006-09-13 | 2008-04-24 | Syngenta Participations Ag | Novel rucola plants with cytoplasmic male sterility (cms) |
| CN101480164B (en) * | 2009-02-18 | 2011-03-23 | 西北农林科技大学 | Method for breeding novel germplasm of Chinese cabbage with purple core |
Non-Patent Citations (4)
| Title |
|---|
| 张德双等: "紫色大白菜育种思路初探", 《长江蔬菜（学术版）》 * |
| 曹华等: "紫红色蔬菜品种", 《蔬菜》 * |
| 胡天华等: "彩色白菜雄性不育系紫A的选育初报", 《浙江农业科学》 * |
| 辛彬等: "青梗菜核基因雄性不育系的转育", 《中国蔬菜》 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170922 |