CN107174536A - 一种大鲵美容护肤品及其制备方法 - Google Patents
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Abstract
本发明提供一种大鲵美容护肤品及其制备方法，包括油相基料和水相基料，油相基料中含有护肤品总重量１－１０％的大鲵胶原蛋白。本发明提供一种大鲵美容护肤品的其制备方法。本发明中的大鲵胶原蛋白其细胞相溶性好，易被皮肤吸收，其内含丰富的高效能营养成份，具有保护、改善、调理皮肤的作用，其性能温和，刺激小，补充皮肤细胞生长所必须营养及维护皮肤的健康，可有效地提高人体皮肤免疫能力，调节新陈代谢，延缓衰老，维持皮肤的健康。
Description
技术领域
本发明提供一种美容化装品，具体地说，是一种含有从大鲵中提取的活性物质的护肤品。
背景技术
胶原蛋白是人体中一种重要的蛋白质，约占人体中所有蛋白质总量的30％ 左右，相当于人体体重的6％，遍及全身各个组织器官。胶原蛋白是组织的支持 物和填充物，参与细胞的迁移、分化及增殖，并与创伤的修复及胚胎的发育密切 相关。胶原蛋白已广泛用于美容化妆品领域。目前临床上应用的胶原蛋白制品 主要是从哺乳动物猪、牛皮肤中获得。这类胶原蛋白的提取处理比较麻烦。为得 到干净卫生的皮肤原材料，屠宰前后需要大量的工作来清洁皮肤，成本相应提高。 而且这类动物胶原蛋白由于分子量较大，细胞相溶性相对较差，存在排异性。
大鲵是一种具有三亿多年演化史的两栖动物，被誉为“活化石”。大鲵具有 很高的保健药用价值和生态价值，其在生物医药、保健品和美容化妆品领域的应 用潜力很大。近几年来，大鲵人工繁殖和养殖越来越受到人们的重视，随着大鲵 人工繁养技术的成熟和推广，大鲵产量显著提高，国家有关部门已批准人工繁养 大鲵子二代可以加工和销售，但目前是市场上没有大鲵深加工产品出现，大鲵在 美容化妆品领域的研究报道极为少见。
发明内容
本发明的目的就在于提供一种含有大鲵有效成份的美容护肤品，其细胞相溶 性好，易被皮肤吸收，具有使皮肤组织内弹性蛋白含量增加、促进衰老细胞再生， 增强皮肤免疫力等效果。
本发明还提供一种大鲵抗皱美白护肤品的制备方法。
为达到上述目的，本发明的实施方案为：一种大鲵美容护肤品，包括油相基料和水相基料，其特征在于，油相基料中含有护肤品总重量1-10％的大鲵胶原蛋 白。
本发明由包括以下重量百分比的组份组成：
油相基料：大鲵胶原蛋白1-10％、十六醇磷酸酯钾1.5％、橄榄油2.5％、棕 榈酸异丙酯5.5％、聚氧乙烯月桂醚-72.0％、维生素B醋酸酯0.4％、碳酸二辛 酯4.5％、聚苯基二甲基硅氧烷2.0％；
水相基料：甘油4.0％、维生素C磷酸酯钠1.4％、Germaben II 0.5％、去离子 水至100％。
本发明的制备方法包括以下步骤：
(1)分别将油相基料和水相基料加热至70 ℃，保持20min；
(2)将水相记料慢慢加人油相记料，搅拌均质；
(3)冷却至30℃， 充分搅拌使之均匀；(4)按量分装，密封保存。
本发明所述大鲵胶原蛋白的提取方法包括以下步骤：
(1)、将大鲵剖杀去内脏，洗净后用绞肉机捣碎；
(2)、用高速组织捣碎机把细胞粉碎，在每毫升悬液中加100μg至1mg溶菌 酶，在37℃下保温10min，细胞被破坏后，使活性物质充分释放到溶液内；
(3)、采用胃蛋白酶在酸性条件下组织制成悬浮液∶乙酸浓度为0.5mol/L、 pH值2～2.5，胃蛋白酶用量0.5～5.0mol/L酶/克组织，温度4℃～15℃；
(4)、悬浮液超速低温离心40min，把收集的沉淀溶解在醋酸中，0.5μm滤膜 过滤，对0.005mol/L醋酸溶液在4℃下透析16h；
(5)、粗样品通过0.5μm滤膜过滤后，提取滤液上层进行色谱分离，色谱条 件：9.4mm×25cm半制备色谱柱，流动相水∶甲醇＝80∶20，进样量300μL，采用 线形梯度的方法洗脱，流速1.5mL/min，检测波长200nm，柱温20℃，收集主峰， 冻干。
本发明中的主要成份大鲵胶原蛋白分子量相对较小，细胞相溶性好，渗透效 果好，容易被人体皮肤、毛孔吸收，对人体皮肤具有保湿、防皱、营养作用，可 令肌肤娇嫩，有光泽，有弹性；十六醇磷酸酯钾为一种乳化剂，是至关重要的护 肤品的基础配方，保证护肤的稳定性、细腻程度及滋润保湿效果；橄榄油富含维 生素A、D、E、K、F，有明显滋润营养肌肤的效果；棕榈酸异丙酯为一种润肤 剂，易被皮肤组织吸收，可滋润皮肤并有助于营养成份向皮肤深处的渗透，赋予 护肤品以良好的涂敷展开性；聚氧乙烯月桂醚一7为一种增稠剂，其可使产品触 感更佳，柔软又光滑细腻；维生素B醋酸酯对肌肤有美白、营养和保湿作用；碳 酸二辛酯，它具有极干爽的肤感和良好的铺展性，可与挥发性硅油相媲美，具有 极佳的皮肤相容性，对皮肤和粘膜的刺激性很低；聚苯基二甲基硅氧烷能渗入皮 肤内部及具有凝水性能；甘油具有保湿性能，使用后有保湿感觉，更使肌肤营养 滋润、充满生机活力。维生素C磷酸酯钠为一种水溶性美白剂，经皮肤吸收后， 能有效地抵抗紫外线侵袭，并可捕获氧自由基，促进胶原蛋白生成，能有效防治 色素沉着，祛除各种皮肤色斑；Germaben II为一种防腐剂，有效对抗革兰氏阴、 革兰氏阳细菌、酵母菌、霉菌，它的作用是保护产品免受微生物污染，在产品保 质期内保持品质的稳定。
以大鲵美容因子胶原蛋白为主复合而成的大鲵美容护肤品，可促进已衰老细 胞的再生技能，强化皮肤免疫能力，使各种问题皮肤得以修复改善，更能延缓衰 老，恢复青春。其内含丰富的高效能营养成份，具有保护、改善、调理皮肤的作 用，其性能温和，刺激小，补充皮肤细胞生长所必须营养及维护皮肤的健康，可 有效地提高人体皮肤免疫能力，调节新陈代谢，延缓衰老，维持皮肤的健康。
具体实施方式
下面给出本发明的三个实施例：
原料名称
实施例1(％) 实施例2(％) 实施例3(％)
油相 原料
大鲵胶原蛋白 1 5 10
十六醇磷酸酯钾 1.5 1.5 1.5
橄榄油 2.5 2.5 2.5
棕榈酸异丙酯 5.5 5.5 5.5
聚氧乙烯月桂醚一7 2.0 2.0 2.0
维生素B醋酸酯 0.4 0.4 0.4
碳酸二辛酯 4.5 4.5 4.5
聚苯基二甲基硅氧烷 2.0 2.0 2.0
水相 原料
甘油 4.0 4.0 4.0
维生素C磷酸酯钠 1.4 1.4 1.4
Germaben II 0.5 0.5 0.5
去离子水 至100 至100 至100
香精 适量 适量 适量。
上述实施例的制备方法是：(1)分别将油相基料和水相记料加热至70℃，保持 20min；(2)将水相基料慢慢加人油记记料，搅拌均质；(3)冷却至30℃，加人香 精，充分搅拌使之均匀；(4)按量分装，密封保存。
其中大鲵胶原蛋白的提取方法是：
①原料及预处理：原料从健康的大鲵上获得(包括大鲵的肉、骨头等)。将 大鲵剖杀去内脏，洗净后用绞肉机捣碎；
②粉碎细胞：用高速组织捣碎机(5000rpm，5min)把细胞粉碎，在每毫升 悬液中加100μg至1mg溶菌酶，在37℃下保温10min，细胞被破坏后，使活性物质 充分释放到溶液内；
③抽取：采用胃蛋白酶在酸性条件下组织制成悬浮液∶乙酸浓度为0.5mol /L，pH值2～2.5，胃蛋白酶用量0.5mol～5.0mol/L酶/克组织，温度4℃～1 5℃。悬浮液超速低温离心40min，把收集的沉淀溶解在醋酸中，0.5μm滤膜过滤， 对0.005mol/L醋酸溶液在4℃下透析16h；
④色谱分离：粗样品通过0.5μm滤膜过滤后，提取滤液上层进行色谱分离， 色谱条件：ZORBAX300SB-C18半制备色谱柱(9.4mm×25cm)，流动相：A水，B 甲醇，水∶甲醇＝80∶20(体积比)，进样量：300μL；采用线形梯度的方法洗脱；流 速：1.5mL/min；检测波长200nm；柱温：20℃。收集主峰，冻干。
Claims (4)
1.一种大鲵美容护肤品及其制备方法，包括油相基料和水相基料，其特征在于，油相基料中含有护肤品总重量1-10％的大鲵胶原蛋白。
2.根据权利要求1所述的大鲵美容护肤品，其特征在于，其由包括以下重量 百分比的组份组成： 油相基料：大鲵胶原蛋白5-20％、十六醇磷酸酯钾1.5％、橄榄油2.5％、棕 榈酸异丙酯5.5％、聚氧乙烯月桂醚一72.0％、维生素B醋酸酯0.4％、碳酸二辛 酯4.5％、聚苯基二甲基硅氧烷2.0％； 水相基料：甘油4.0％、维生素C磷酸酯钠1.4％、Germaben II 0.5％、去离子 水至100％。
3.根据权利要求1所述的大鲵美容护肤品，其特征在于，所述大鲵胶原蛋白 的提取方法包括以下步骤： (1)、将大鲵剖杀去内脏，洗净后用绞肉机捣碎； (2)、用高速组织捣碎机把细胞粉碎，在每毫升悬液中加100μg至1mg溶菌 酶，在37℃下保温10min，细胞被破坏后，使活性物质充分释放到溶液内； (3)、采用胃蛋白酶在酸性条件下组织制成悬浮液：乙酸浓度为0.5mol/L、 pH值2～2.5，胃蛋白酶用量0.5～5.0mol/L酶/克组织，温度4℃～15℃；(4)、悬浮液超速低温离心40min，把收集的沉淀溶解在醋酸中，0.5μm滤膜 过滤，对0.005mol/L醋酸溶液在4℃下透析16h； (5)、粗样品通过0.5μm滤膜过滤后，提取滤液上层进行色谱分离，色谱条 件：9.4mm×25cm半制备色谱柱，流动相水∶甲醇＝80∶20，进样量300μL，采用线 形梯度的方法洗脱，流速1.5mL/min，检测波长200nm，柱温20℃，收集主峰， 冻干。
4.根据权利要求1或2所述的大鲵美容护肤品的制备方法，其特征在于，包 括以下步骤：(1)分别将油相基料和水相记料加热至70℃，保持20min；(2)将水相 记料慢慢加人油相基料中，搅拌均质；(3)冷却至30℃，充分搅拌使之均匀；(4) 按量分装，密封保存。
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| CN107625722A (zh) * | 2017-10-16 | 2018-01-26 | 徐宏伟 | 一种植物提取的美容养颜的护肤品及其制备工艺 |
| CN108272651A (zh) * | 2018-03-13 | 2018-07-13 | 汉中天成生物工程有限公司 | 大鲵皮胶原蛋白的应用及含大鲵皮胶原蛋白的面膜及其制备方法 |
| CN109330969A (zh) * | 2018-11-12 | 2019-02-15 | 汉中市汉鲵食品加工有限公司 | 含大鲵油的中药洗发护发剂 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107625722A (zh) * | 2017-10-16 | 2018-01-26 | 徐宏伟 | 一种植物提取的美容养颜的护肤品及其制备工艺 |
| CN108272651A (zh) * | 2018-03-13 | 2018-07-13 | 汉中天成生物工程有限公司 | 大鲵皮胶原蛋白的应用及含大鲵皮胶原蛋白的面膜及其制备方法 |
| CN109330969A (zh) * | 2018-11-12 | 2019-02-15 | 汉中市汉鲵食品加工有限公司 | 含大鲵油的中药洗发护发剂 |
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