CN107164289A - A kind of microorganism formulation of efficient degradation deodorization and preparation method thereof - Google Patents
A kind of microorganism formulation of efficient degradation deodorization and preparation method thereof Download PDFInfo
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- CN107164289A CN107164289A CN201710586365.0A CN201710586365A CN107164289A CN 107164289 A CN107164289 A CN 107164289A CN 201710586365 A CN201710586365 A CN 201710586365A CN 107164289 A CN107164289 A CN 107164289A
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- bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a kind of microorganism formulation of efficient degradation deodorization and preparation method thereof, following microorganism fungus kind is contained in the microorganism formulation：Bacillus subtilis, Bacillus acidi lactici, Bacillus foecalis alkaligenes, aspergillus niger, microphenomenon of degradation of aniline, mucus colloid bacillus cereus and Bacillus circulans, the microorganism formulation also contain bacterium powder solid matrix and fermentation medium；By reasonable preparation technology, by adding microbial fixed carrier, enzyme, the strain of dominant microflora can be formed in biochemical processing procedure of sewage by various, biomass is big, and breeding is fast.Advantage can be formed in dyeing and printing sewage processing system rapidly, thus rapidly, sewage of targetedly degrading；Purification efficiency is high, and good purification.
Description
Technical field
The present invention relates to microbial technology field, and in particular to a kind of microorganism formulation of efficient degradation deodorization and its preparation
Method.
Background technology
The core of Sewage Biological Treatment is to cultivate to tame out to be adapted to need the superior microorganism flora of sewage feature to be processed,
And keep and the adaptable microbial biomass of pollutant concentration, property and activity, superior microorganism flora is in optimal degraded
Activity, gives full play to its degradation function, and the biological treatment capabilities and treatment effeciency of system are improved to greatest extent.Current sewage
Biological treatment, however it remains many defects, biochemical wastewater treatment is inefficient.
The content of the invention
In view of this, it is an object of the invention to provide a kind of microorganism formulation of efficient degradation deodorization and preparation method thereof.
In order to solve the above-mentioned technical problem, the present invention is adopted the following technical scheme that：
Contain following microorganism fungus kind in a kind of microorganism formulation of efficient degradation deodorization, the microorganism formulation：Withered grass
Bacillus, Bacillus acidi lactici, Bacillus foecalis alkaligenes, aspergillus niger, microphenomenon of degradation of aniline, mucus colloid bacillus cereus and ring-type gemma bar
Bacterium, the microorganism formulation also contains bacterium powder solid matrix and fermentation medium.
The present invention provides a kind of preparation side of the microorganism formulation of efficient degradation deodorization for another technical problem to be solved
Method, comprises the following steps：
1) by each microorganism fungus kind, separately made dilution bacterium solution is divided obtained dilution bacterium solution by plate streaking
From the culture medium in erlenmeyer flask used in addition seed selection is inoculated with the single strain obtained after separation, is then placed in incubator
Individually culture；Microbial strain culture Jing Guo above-mentioned steps is carried out to be mixed to form mixed bacterial culture；
2) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, stir 10 minutes, be stirred end after send out
Ferment culture 30 days, it is standby；
3) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, carry out continuous 5 days, be passed through 1 every 15 minutes
It is standby after minute pressure is 0.2MPa purity oxygen fermentation process；
4) by step 2) and step 3) preparation for preparing according to volume ratio is to be mixed at 2.5: 1, and micro- life is added after mixing
After thing carrier and enzyme preparation, microbial fermentation nutrient solution is sprayed, fermented and cultured carries out vacuum freeze drying, i.e., after 15 days again
Obtain the microorganism formulation.
Further, the step 1) in, by weight, the consumption during culture mixing of each strain is respectively：It is withered
Careless bacillus 10-15 parts, 13-17 parts of Bacillus acidi lactici, 11-19 parts of Bacillus foecalis alkaligenes, 12-16 parts of aspergillus niger, microphenomenon of degradation of aniline
11-17 parts, 21-33 parts of mucus colloid bacillus cereus, 7-11 parts of Bacillus circulans.
Further, the solid matrix is the mixture of peat powder and calcium carbonate, and the mass fraction of wherein calcium carbonate is
13%.
Further, the microbe carrier is made up of wheat-middlings and dregs of beans.
Further, the enzyme preparation is by alpha-amylase, β-glucolase, glucoamylase, lipase, cellulase
With the one or more composition of protease.
Beneficial effects of the present invention are：The microorganism formulation of the present invention is significantly carried by adding microbial fixed carrier
The high adhesion amount of microorganism.Alpha-amylase, β-glucolase, glucose is selectively added during the fermentation simultaneously to form sediment
The enzymes such as powder enzyme, lipase, cellulase, protease, can be abolished and non-starch in catalytic matrix carrier in the decomposition of cell membrane
The hydrolysis of polysaccharide (NSP), contributes to microorganism fast decoupled to utilize the nutriment in fermentation substrate, promotes microorganism growth numerous
Grow；The addition of exogenous enzymes simultaneously helps to improve the activity of microorganism endogenous enzymes, supplements the deficiency of endogenous enzymes, promotes various effective
The secretion and release of enzyme so that in microorganism formulation in addition to containing a large amount of beneficial microbes, are also compounded with various effective enzymes.
These enzymes additionally aid catalysis by microorganism adsorption in extracellular organic contamination after input dyeing and printing sewage biochemical treatment system
The hydrolysis and conversion of composition, beneficial to microorganism panning, accelerate microbial reproduction speed, improve the degradation rate of polluter；This
Invention can form the strain of dominant microflora by various in biochemical processing procedure of sewage, and biomass is big, and breeding is fast.It can exist rapidly
Form advantage in dyeing and printing sewage processing system, thus rapidly, sewage of targetedly degrading, reach the effect of high-efficient purification.
Embodiment
Embodiment 1
Contain following microorganism fungus kind in a kind of microorganism formulation of efficient degradation deodorization, the microorganism formulation：Withered grass
Bacillus, Bacillus acidi lactici, Bacillus foecalis alkaligenes, aspergillus niger, microphenomenon of degradation of aniline, mucus colloid bacillus cereus and ring-type gemma bar
Bacterium, the microorganism formulation also contains bacterium powder solid matrix and fermentation medium.
The present invention provides a kind of preparation side of the microorganism formulation of efficient degradation deodorization for another technical problem to be solved
Method, comprises the following steps：
1) by each microorganism fungus kind, separately made dilution bacterium solution is divided obtained dilution bacterium solution by plate streaking
From the culture medium in erlenmeyer flask used in addition seed selection is inoculated with the single strain obtained after separation, is then placed in incubator
Individually culture；Microbial strain culture Jing Guo above-mentioned steps is carried out to be mixed to form mixed bacterial culture；
2) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, stir 10 minutes, be stirred end after send out
Ferment culture 30 days, it is standby；
3) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, carry out continuous 5 days, be passed through 1 every 15 minutes
It is standby after minute pressure is 0.2MPa purity oxygen fermentation process；
4) by step 2) and step 3) preparation for preparing according to volume ratio is to be mixed at 2.5: 1, and micro- life is added after mixing
After thing carrier and enzyme preparation, microbial fermentation nutrient solution is sprayed, fermented and cultured carries out vacuum freeze drying, i.e., after 15 days again
Obtain the microorganism formulation.
Further, the step 1) in, by weight, the consumption during culture mixing of each strain is respectively：It is withered
Careless 10 parts of bacillus, 13 parts of Bacillus acidi lactici, 11 parts of Bacillus foecalis alkaligenes, 12 parts of aspergillus niger, 11 parts of microphenomenon of degradation of aniline, mucus colloid
21 parts of bacillus, 7 parts of Bacillus circulans.
Further, the solid matrix is the mixture of peat powder and calcium carbonate, and the mass fraction of wherein calcium carbonate is
13%.
Further, the microbe carrier is made up of wheat-middlings and dregs of beans.
Further, the enzyme preparation is by alpha-amylase, β-glucolase, glucoamylase, lipase, cellulase
With the one or more composition of protease.
Embodiment 2
Contain following microorganism fungus kind in a kind of microorganism formulation of efficient degradation deodorization, the microorganism formulation：Withered grass
Bacillus, Bacillus acidi lactici, Bacillus foecalis alkaligenes, aspergillus niger, microphenomenon of degradation of aniline, mucus colloid bacillus cereus and ring-type gemma bar
Bacterium, the microorganism formulation also contains bacterium powder solid matrix and fermentation medium.
The present invention provides a kind of preparation side of the microorganism formulation of efficient degradation deodorization for another technical problem to be solved
Method, comprises the following steps：
1) by each microorganism fungus kind, separately made dilution bacterium solution is divided obtained dilution bacterium solution by plate streaking
From the culture medium in erlenmeyer flask used in addition seed selection is inoculated with the single strain obtained after separation, is then placed in incubator
Individually culture；Microbial strain culture Jing Guo above-mentioned steps is carried out to be mixed to form mixed bacterial culture；
2) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, stir 10 minutes, be stirred end after send out
Ferment culture 30 days, it is standby；
3) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, carry out continuous 5 days, be passed through 1 every 15 minutes
It is standby after minute pressure is 0.2MPa purity oxygen fermentation process；
4) by step 2) and step 3) preparation for preparing according to volume ratio is to be mixed at 2.5: 1, and micro- life is added after mixing
After thing carrier and enzyme preparation, microbial fermentation nutrient solution is sprayed, fermented and cultured carries out vacuum freeze drying, i.e., after 15 days again
Obtain the microorganism formulation.
Further, the step 1) in, by weight, the consumption during culture mixing of each strain is respectively：It is withered
Careless 15 parts of bacillus, 17 parts of Bacillus acidi lactici, 19 parts of Bacillus foecalis alkaligenes, 16 parts of aspergillus niger, 17 parts of microphenomenon of degradation of aniline, mucus colloid
33 parts of bacillus, 11 parts of Bacillus circulans.
Further, the solid matrix is the mixture of peat powder and calcium carbonate, and the mass fraction of wherein calcium carbonate is
13%.
Further, the microbe carrier is made up of wheat-middlings and dregs of beans.
Further, the enzyme preparation is by alpha-amylase, β-glucolase, glucoamylase, lipase, cellulase
With the one or more composition of protease.
Embodiment 3
Contain following microorganism fungus kind in a kind of microorganism formulation of efficient degradation deodorization, the microorganism formulation：Withered grass
Bacillus, Bacillus acidi lactici, Bacillus foecalis alkaligenes, aspergillus niger, microphenomenon of degradation of aniline, mucus colloid bacillus cereus and ring-type gemma bar
Bacterium, the microorganism formulation also contains bacterium powder solid matrix and fermentation medium.
The present invention provides a kind of preparation side of the microorganism formulation of efficient degradation deodorization for another technical problem to be solved
Method, comprises the following steps：
1) by each microorganism fungus kind, separately made dilution bacterium solution is divided obtained dilution bacterium solution by plate streaking
From the culture medium in erlenmeyer flask used in addition seed selection is inoculated with the single strain obtained after separation, is then placed in incubator
Individually culture；Microbial strain culture Jing Guo above-mentioned steps is carried out to be mixed to form mixed bacterial culture；
2) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, stir 10 minutes, be stirred end after send out
Ferment culture 30 days, it is standby；
3) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial is trained after seed selection
Foster thing is inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, carry out continuous 5 days, be passed through 1 every 15 minutes
It is standby after minute pressure is 0.2MPa purity oxygen fermentation process；
4) by step 2) and step 3) preparation for preparing according to volume ratio is to be mixed at 2.5: 1, and micro- life is added after mixing
After thing carrier and enzyme preparation, microbial fermentation nutrient solution is sprayed, fermented and cultured carries out vacuum freeze drying, i.e., after 15 days again
Obtain the microorganism formulation.
Further, the step 1) in, by weight, the consumption during culture mixing of each strain is respectively：It is withered
Careless 12 parts of bacillus, 15 parts of Bacillus acidi lactici, 15 parts of Bacillus foecalis alkaligenes, 14 parts of aspergillus niger, 14 parts of microphenomenon of degradation of aniline, mucus colloid
27 parts of bacillus, 9 parts of Bacillus circulans.
Further, the solid matrix is the mixture of peat powder and calcium carbonate, and the mass fraction of wherein calcium carbonate is
13%.
Further, the microbe carrier is made up of wheat-middlings and dregs of beans.
Further, the enzyme preparation is by alpha-amylase, β-glucolase, glucoamylase, lipase, cellulase
With the one or more composition of protease.
Experimental example
Experimental subjects：3 tons of identical sewage, are divided into 3 groups, every group of 1 ton of sewage according to experimental method.
Experimental method and time：Control group 1 uses common microorganism formulation, and control group 2 uses the microorganism system of import
Agent, experimental group is using the microorganism formulation of the efficient degradation deodorization of the present invention, and experimental period is 15 days, is recorded the 1st day and the 15th
It situation situation.
Effect compares after three groups of microorganism formulations deliver the 1st, as a result see the table below
Effect compares after three groups of microorganism formulations deliver the 15th, as a result see the table below
By experiment, three groups of situations have highly significant sex differernce, and the microorganism formulation of efficient degradation deodorization of the invention is net
Change efficiency high, and good purification.
Certainly, it is the representative instance of the present invention above, in addition, the present invention can also have other a variety of specific implementations
The technical scheme of mode, all use equivalent substitutions or equivalent transformation formation, all falls within the scope of protection of present invention.
Claims (6)
1. a kind of microorganism formulation of efficient degradation deodorization, it is characterised in that contain following microorganism in the microorganism formulation
Strain：Bacillus subtilis, Bacillus acidi lactici, Bacillus foecalis alkaligenes, aspergillus niger, microphenomenon of degradation of aniline, mucus colloid bacillus cereus and ring
Shape bacillus, the microorganism formulation also contains bacterium powder solid matrix and fermentation medium.
2. a kind of preparation method of the microorganism formulation of efficient degradation deodorization, it is characterised in that comprise the following steps：
1) by each microorganism fungus kind, separately made dilution bacterium solution is separated obtained dilution bacterium solution by plate streaking,
The culture medium used in seed selection is added in erlenmeyer flask, the single strain obtained after separation is inoculated with, is then placed in incubator individually
Culture；Microbial strain culture Jing Guo above-mentioned steps is carried out to be mixed to form mixed bacterial culture；
2) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial culture after seed selection
Be inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, stir 10 minutes, be stirred end after fermentation training
Support 30 days, it is standby；
3) bacterium powder solid matrix and fermentation medium are added in fermentation tank, half step 1 is taken) mixed bacterial culture after seed selection
Be inoculated into fermentation tank, temperature be 35 DEG C, pH value be 7.2 under conditions of, carry out continuous 5 days, be passed through every 15 minutes 1 minute
It is standby after pressure is 0.2MPa purity oxygen fermentation process；
4) by step 2) and step 3) preparation for preparing according to volume ratio is to be mixed at 2.5: 1, and microorganism load is added after mixing
After body and enzyme preparation, microbial fermentation nutrient solution is sprayed, fermented and cultured carries out vacuum freeze drying, produce institute after 15 days again
State microorganism formulation.
3. a kind of preparation method of the microorganism formulation of efficient degradation deodorization according to claim 2, it is characterised in that institute
State step 1) in, by weight, the consumption during culture mixing of each strain is respectively：10-15 parts of bacillus subtilis, breast
13-17 parts of acidfast bacilli, 11-19 parts of Bacillus foecalis alkaligenes, 12-16 parts of aspergillus niger, 11-17 parts of microphenomenon of degradation of aniline, mucus colloid gemma
21-33 parts of bacillus, 7-11 parts of Bacillus circulans.
4. a kind of microorganism formulation of efficient degradation deodorization according to claim 1, it is characterised in that the solid matrix
For peat powder and the mixture of calcium carbonate, the wherein mass fraction of calcium carbonate is 13%.
5. a kind of preparation method of the microorganism formulation of efficient degradation deodorization according to claim 2, it is characterised in that institute
Microbe carrier is stated to be made up of wheat-middlings and dregs of beans.
6. a kind of preparation method of the microorganism formulation of efficient degradation deodorization according to claim 2, it is characterised in that institute
State the one kind or many of enzyme preparation by alpha-amylase, β-glucolase, glucoamylase, lipase, cellulase and protease
Plant composition.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110282751A (en) * | 2019-06-26 | 2019-09-27 | 天津市蓟县绿普生蔬菜种植有限公司 | A kind of biological deodorant |
| CN112159060A (en) * | 2020-09-29 | 2021-01-01 | 重庆大学 | Microorganism-combined plant-type composite deodorant for black-odor rivers and preparation method thereof |
| CN113980802A (en) * | 2021-10-12 | 2022-01-28 | 湖北中向环保科技有限公司 | Sewage treatment microbial preparation, production system and production method |
| CN114437957A (en) * | 2020-11-05 | 2022-05-06 | 中石化石油工程技术服务有限公司 | Degradable microbial composition for environment-friendly toilet and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110282751A (en) * | 2019-06-26 | 2019-09-27 | 天津市蓟县绿普生蔬菜种植有限公司 | A kind of biological deodorant |
| CN112159060A (en) * | 2020-09-29 | 2021-01-01 | 重庆大学 | Microorganism-combined plant-type composite deodorant for black-odor rivers and preparation method thereof |
| CN114437957A (en) * | 2020-11-05 | 2022-05-06 | 中石化石油工程技术服务有限公司 | Degradable microbial composition for environment-friendly toilet and application thereof |
| CN113980802A (en) * | 2021-10-12 | 2022-01-28 | 湖北中向环保科技有限公司 | Sewage treatment microbial preparation, production system and production method |
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