CN102173879A - Method for producing biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residue - Google Patents
Method for producing biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residue Download PDFInfo
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- CN102173879A CN102173879A CN2010105998196A CN201010599819A CN102173879A CN 102173879 A CN102173879 A CN 102173879A CN 2010105998196 A CN2010105998196 A CN 2010105998196A CN 201010599819 A CN201010599819 A CN 201010599819A CN 102173879 A CN102173879 A CN 102173879A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to a method for producing a biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residues, belonging to the field of microbial fertilizers. The biological potassium fertilizer is produced by adopting a solid-state fermentation process and carrying out solid-state fermentation on a mixture of the cellulose fermented waste mycelium, the biogas residues and potassium mineral powder by utilizing potassium bacteria. The method has the advantages of low cost, high fertilizer efficiency and obvious yield increase effect, has double advantages of environmental protection and resource utilization because a large amount of the celluloses fermented waste mycelium are utilized, and is suitable for large-scale production and popularization.
Description
Technical field
The invention belongs to the microbial fertilizer field in the biological fertilizer field, relating to a kind of is the method for main material production biological potassium fertilizer with cellulase fermentations waste mycelia and natural pond slag.
Background technology
Filamentous fungus is the important production bacterial classification of fermentation industry, is used in the middle of the production of multiple products such as methylene-succinic acid, citric acid, fungal amylase, cellulase, Sunmorl N 60S, penicillin.Current, the production of cellulase has become the fourth-largest enzyme of China, and its fermentation mode changes liquid state fermentation into from solid state fermentation, and a large amount of waste mycelia serious environment pollutions that produce in its fermenting process are demanded urgently handling.At present, the fermentation waste mycelia can extract (Du Yumin, Cai Jun, Yang Jianhong, Qiu Yanlin such as chitosan, ergosterol, protein.The method of separating husk glycan from the lemon acid waste mycelium.Chinese patent, CN 1260253C; Tan Tianwei, Luo Hui, Qi Yizheng.A kind of method of utilizing waste mycelia to extract ergosterol.Chinese patent, CN 1118472C; Liu Jianjun, Li Piwu.From the terreus waste mycelia that obtained by producing itaconic acid by fermentation produces, extract the method for chitosan, Chinese patent, CN 101538335A), but the effective treating method of the not mentioned cellulase fermentations waste mycelia of above-mentioned document, and the method for being addressed is because of its processing cost height, also be unsuitable for large-scale application, a large amount of waste mycelias still can not get effective solution.In waste mycelia, exist the chitin and the 30% above protein that account for its dry weight 20-30%, nutritional sufficiency very is suitable for the production of organic fertilizer.Wherein chitin produces chitosan after microbiological deterioration.Than the chitosan in shrimp, crab shell source, the radicula byssoidea chitosan has wide material sources, advantages such as seasonal effect are stablized, are not subjected to quality safety.In addition, cellulase activity remaining in the cellulase waste mycelia can be hydrolyzed to cellulose substances, improves the utilising efficiency of cellulosic waste.
The natural pond slag generally can be used as bio-feritlizer, but its fertilizer efficiency is lower and unstable.Consider and wherein still contain the part lignocellulose that utilizing the natural pond slag to prepare bio-feritlizer has had relevant patent disclosure.Application for patent 200810147682.3 discloses the method with preparation of microorganism organic fertilizer from methane residue.Its component is: organic fertilizer 60-80%, humic acid fertilizer 30-35%, microbial function bacterium (bacillus laterosporus and subtilis) 3-4%, the micro-1-1.5% of biogas slag after harmless treatment.Said components can obtain bio-feritlizer after stirring.Application for patent 200810219155.9 (CN101423425) discloses natural pond slag for comprehensive utilization and has produced active bio-organic fertilizer and method thereof.Its raw material weight proportioning is: natural pond slag: mud organic substance: the Herba Eichhorniae butt: molasses fermented waste water: EM bacterium=55-62: 15-20: 12.5-15: 10-14: 0.5-1.0.The active bio-organic fertilizer that is obtained can obviously promote plant growth, improves output.Application for patent 201010011344.4 discloses biogas residue organic fertilizer and preparation method thereof, with the natural pond slag is main raw material (consumption is greater than 80%), cooperates the back to make fertilizer after aerobic Secondary Fermentation, air-dry, curing in shallow pond with humic acids, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer and trace element.Application for a patent for invention book 200810025668.6 discloses a kind of production method of organic biofertilizer.This method comprises the steps: that mainly the raw material and the water that at first animal excrement, wooden broken end, waste residue, additional material and bacterial classification (genus bacillus is a yeast) are constituted mix; Secondly, mixture is carried out the normal temperature compost fermentation 40-55 days and charged into air 2-4 time, become fertilizer when water content reaches 20%-25% in mixture.Though above-mentioned patent utilizes the natural pond slag as raw material, specific aim is not done further degradation treatment to main component Mierocrystalline cellulose wherein, causes that the lignocellulose hydrolysis is insufficient in the slag of natural pond, fertilizer efficiency is not high, and practical application effect is undesirable.
Application for patent 200810025668.6 discloses a kind of production method of organic biofertilizer, with animal excrement, wooden broken end, waste residue, replenish and carry out the normal temperature compost fermentation after raw material that material and bacterial classification constitute and water mix, fermentation time is 40-55 days, bubbling air 2-4 time in mixture therebetween, when water content is 20-25% fertilizer.The used bacterial classification of this method is yeast and genus bacillus, and gained fertilizer nutritive ingredient is uncertain, and because of being conventional compost, potassium bacterium living beings vigor is uncertain, the fertilizer efficiency instability, and living bacteria count is difficult to up to standard, is unfavorable for commercialization.Application for patent 200410088855.0 (CN 1257267C) discloses a kind of potassium bacterium and has utilized this bacterium to produce the method for microorganism manure strain agent, provide novel bacterial and new solution to produce the microbial strains agent, the method that it adopted is a liquid fermentation method, and made microbial inoculum is used with conventional fertilizer again.This invention has better solved the effective viable bacteria concentration problems in the biological potassium fertilizer, but not mentioned waste ' s reclamation problem, and production cost is higher, is unfavorable for large-scale promotion.
The invention discloses a kind of method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer, utilize two kinds of agriculture and industry wastes to produce biological potassium fertilizer, can reduce production costs greatly, and solved the natural pond slag efficiently utilize problem, help the promotion and application of extensive biogas engineering.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer.
The present invention is made up of following steps: the pre-treatment of (1) natural pond slag: adopt the cellulase fermentations waste mycelia to mix with the natural pond slag, utilize the Mierocrystalline cellulose in the cellulase activity hydrolysis natural pond slag wherein; (2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with wherein cellulase fermentations waste mycelia, urea, calcium superphosphate, Repone K, potassium primary phosphate, potassium breeze, initial water content 60%-80%, pH 6-8. (3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering: two kinds of method for culturing bacteria can obtain from open source literature, its key step is shakes bottle back and cultivates acquisition shake-flask seed (first order seed) inoculating through the thalline of slant culture, shake-flask seed is inoculated into carries out amplification culture (secondary seed) in the fermentor tank again; (4) solid state fermentation is produced biological potassium fertilizer: Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water respectively, proportionally insert in the solid-state fermentation culture medium.The inoculation back mixes in fermentation vat, and fermentation time is 2-8 days; (5) drying.
The used bacterial classification of the present invention: Bacillus circulans and bacillusmusilaginosiengineering obtain from Chinese common micro-organisms culture presevation administrative center (CGMCC).Wherein Bacillus circulans can be numbered 1.383,1.553 for CGMCC, 1.554,1.2411,1.2394,1.2008 one of them; Bacillusmusilaginosiengineering can be numbered 1.231,1.232 for CGMCC, 1.2326 one of them.
If no special instructions, the raw material consumption is weight part among the present invention.
Mention the cellulase fermentations waste mycelia in the literary composition and be meant, the liquid fermentation ends of cellulase is after the resulting radicula byssoidea of filtration treatment.Related fungi is mainly the fungi of cellulase-producing, and is mould as aspergillus, mould and wood.
Natural pond slag mentioned in the literary composition is meant, utilize feces of livestock and poultry and cellulose waste to produce the solid ingredient of the waste gained after solid-liquid separation that discharges in the process for raw material, especially, be meant with the agricultural crop straw to be the solid ingredient that main raw material is produced the waste gained after solid-liquid separation that discharges in the biogas process.
Mentioned potassium breeze is meant one or more mixture of potassium felspar sand breeze, nepheline breeze, polyhalite breeze, alunite breeze in the literary composition.
Biological potassium fertilizer mentioned in the literary composition is meant: living bacteria count meets the solid silicate bacterial fertilizer of Chinese NY227-94 agricultural industry criteria regulation.
Concrete technical scheme of the present invention is as follows:
(1) pre-treatment of natural pond slag: cellulase fermentations waste mycelia 1-5 part, natural pond slag 2-6 part, regulating water content behind the thorough mixing in fermentation vat is 50%-70% (w/w), and adjusting pH is 4-6, handles 1-5 days under 20 ℃ of-50 ℃ of conditions, and stirring is 3-8 time therebetween.
(2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with wherein cellulase fermentations waste mycelia 100-200 part, urea 3-8 part, calcium superphosphate 5-10 part, Repone K 0-3 part, potassium primary phosphate 2-5 part, potassium breeze 10-20 part, initial water content 60%-80%, pH 6-8.Above-mentioned substratum is standby after 30 minutes through 121 ℃ of sterilizations.
(3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering.This step comprises that the slant medium of slant medium cultivation, liquid state fermentation cultivation and the bacillusmusilaginosiengineering of Bacillus circulans is cultivated, two portions are cultivated in liquid state fermentation.
1. the cultivation of described Bacillus circulans:
Slant medium: peptone 1, extractum carnis 0.3, sodium-chlor 0.5, agar 2, water 100, pH 7.0.Thalline on the above-mentioned slant medium in incubator 32 ℃ be cultured to a large amount of preparations that are used for Bacillus circulans after the inclined-plane covers with.
Liquid nutrient medium: starch 5, sucrose 1, CaCO
31, FeCl
30.1, soybean cake powder 0.5, ammonium sulfate 2, potassium primary phosphate 1, sal epsom 0.5, tap water 1000, pH 7.2.
Shake-flask seed is cultivated: the shaking table culture condition is: 200 rev/mins of rotating speeds, temperature 35-40 ℃, incubation time 20-30 hour acquisition shake-flask seed.
Fermentor tank seed culture: in fermentor tank, insert shake-flask seed with 10% (v/v) inoculum size, rotating speed 300-400 rev/min, culture temperature 35-40 ℃, dissolved oxygen is controlled at more than 20%, air flow is that 1: 1 (volume ratio is a benchmark with the liquid amount) cultivates 15-28 hour acquisition secondary seed.
2. the cultivation of described bacillusmusilaginosiengineering:
Slant medium: sucrose 10, SODIUM PHOSPHATE, MONOBASIC 2, sal epsom 0.5, ammonium sulfate 2, iron(ic) chloride 0.05, lime carbonate 1.0, yeast powder 2, agar 20 (inclined-plane), water 1000,7.0,121 ℃ of sterilizations of pH 30 minutes.Thalline is used for liquid shaking bottle seed preparation being cultured in 32 ℃ of incubators on the above-mentioned slant medium after the inclined-plane covers with.
Liquid nutrient medium: starch 7, sucrose 1, CaCO
31, FeCl
30.1, soybean cake powder 0.5, ammonium sulfate 2, potassium primary phosphate 1, sal epsom 0.5, tap water 1000, pH 7.2.
The shake-flask seed culture condition is: 200 rev/mins of shaking speed, temperature 30-35 ℃, incubation time 10-15 hour acquisition shake-flask seed.
Fermentor tank seed culture: insert shake-flask seed to fermentor tank with 10% (v/v) inoculum size, rotating speed 300-400 rev/min, culture temperature 30-35 ℃, dissolved oxygen is controlled at more than 20%, air flow is that 1: 1 (volume ratio is a benchmark with the liquid amount) cultivates 15-24 hour acquisition secondary seed.
(4) solid state fermentation.Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water respectively, insert in the solid-state fermentation culture medium according to following ratio.The compound method of solid-state fermentation culture medium is seen step (2).
Bacillus circulans seed liquor 5-10, bacillusmusilaginosiengineering 5-10, solid-state fermentation culture medium 100-250.The inoculation back mixes in fermentation vat, and controlled temperature is 25-40 ℃, and humidity is 90%-95%, and fermentation time is 2-8 days, and stirring is 3-10 time therebetween.
(5) drying.Solid state fermentation is dried the gained fermentation fertilizer or is dried after finishing under 40-60 ℃ of condition, control its water content and can obtain the biological potassium fertilizer product at 20-30%.
The invention has the beneficial effects as follows: adopt cellulase activity remaining in the cellulase fermentations waste mycelia to handle the natural pond slag first, wherein the lignocellulose of can partly degrading improves the microorganism utilization ratio of natural pond slag; The fermentable sugars that this process produced can be done carbon source and promote institute's microbe inoculation growth, saves commodity carbon sources (as glucose, starch etc.) cost, significantly improves the living bacteria count in the fertilizer under no additional carbon situation; Partial reduction sugar and protein in the waste mycelia can be used as required carbon source of microbial fermentation and nitrogenous source; Chitin/chitosan in the waste mycelia can be used as fertilizer slow-release formulation, guarantees that fertilizer efficiency is lasting.The present invention adopts the cellulase fermentations waste mycelia to improve the availability of natural pond slag, has solved natural pond slag as fertilizer sources fertilizer efficiency instability, problem that the commercialization difficulty is big.Implement by the present invention program, can solve a slag fertilizer commercial difficult problem in natural pond in the big-and-middle-sized biogas engineering, can obviously improve the economic benefit of these engineerings.
Embodiment
Below in conjunction with embodiment invention is further specified, but the present invention is not limited only to this.
Embodiment 1
A kind of method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer, it comprises the steps: the pre-treatment of (1) natural pond slag: 2 parts of cellulase fermentations waste mycelias, 3 parts of natural pond slags, regulating water content behind the thorough mixing in fermentation vat is 60% (w/w), regulating pH is 5, handled 3 days under 30 ℃ of conditions, stirring is 6 times therebetween.
(2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with 120 parts of wherein cellulase fermentations waste mycelias, 4 parts in urea, and 6 parts of calcium superphosphate, 1 part in Repone K, 3 parts of potassium primary phosphates, 15 parts of potassium breezes, initial water content 70%, pH 7.2.Above-mentioned substratum is standby after 30 minutes through 121 ℃ of sterilizations.
(3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering.
1. Bacillus circulans and bacillusmusilaginosiengineering are being activated on the slant medium respectively separately.
2. treat that the inclined-plane covers with the back and inserts respectively in separately the liquid nutrient medium with inoculating needle and carry out shake-flask culture.Bacillus circulans liquid nutrient medium: starch 5, sucrose 1, CaCO
31, FeCl
30.1, soybean cake powder 0.5, ammonium sulfate 2, potassium primary phosphate 1, sal epsom 0.5, tap water 1000, pH 7.2.Bacillusmusilaginosiengineering liquid nutrient medium: starch 7, sucrose 1, CaCO
31, FeCl
30.1, soybean cake powder 0.5, ammonium sulfate 2, potassium primary phosphate 1, sal epsom 0.5, tap water 1000, pH 7.2.
3. shake-flask seed is cultivated: be inoculated in the triangular flask that liquid nutrient medium (through 121 ℃ of sterilizations 30 minutes) are housed shaking table (the freezing shaking table of HQL300 constant temperature (section of section instrument technical development limited liability company in the Wuhan) cultivation on (300 milliliters triangular flask pack into liquid nutrient medium 100 milliliters) back with the thalline on a small amount of inclined-plane of inoculating needle picking.The Bacillus circulans culture condition is: 200 rev/mins of rotating speeds, and 35 ℃ of temperature, incubation time obtained shake-flask seed in 20 hours.The bacillusmusilaginosiengineering culture condition is: 200 rev/mins of rotating speeds, and 32 ℃ of temperature, incubation time obtained shake-flask seed in 12 hours.
4. fermentor tank seed culture: pack into 70 liters of liquid nutrient mediums of 100 liters of fermentor tanks, 4 milliliters of defoamers, 30 minutes postcooling of 121 ℃ of sterilizations insert shake-flask seed with 10% (v/v) inoculum size.The Bacillus circulans culture condition: 300 rev/mins of rotating speeds, 35 ℃ of culture temperature, dissolved oxygen is controlled at more than 20%, and air flow is that 1: 1 (volume ratio is a benchmark with the liquid amount) cultivates 22 hours acquisition secondary seeds; The bacillusmusilaginosiengineering culture condition is: 400 rev/mins of rotating speeds, and 33 ℃ of culture temperature, dissolved oxygen is controlled at more than 20%, and air flow is that 1: 1 (volume ratio is a benchmark with the liquid amount) cultivates 18 hours acquisition secondary seeds.Cell concentration reaches 10-15 more than hundred million/milliliter through dull and stereotyped live bacterial count in two kinds of bacterium seed liquor, in follow-up work as the solid state fermentation seed.
(4) solid state fermentation: Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water, insert in the solid-state fermentation culture medium according to following ratio.Bacillus circulans seed liquor 10, bacillusmusilaginosiengineering seed liquor 10, solid-state fermentation culture medium 100.The inoculation back mixes in fermentation vat, and controlled temperature is 32 ℃, and humidity is 90%, and fermentation time is 5 days, and stirring is 8 times therebetween.
(5) drying: with the gained fermentation fertilizer under 50 ℃ of conditions with oven dry, controlling moisture content is 25% promptly to get biological potassium fertilizer.Living bacteria count is hundred million of 4-5/gram in the biological potassium fertilizer, and assorted bacterium rate is lower than 10%.
Embodiment 2
A kind of method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer, it comprises the steps: the pre-treatment of (1) natural pond slag: 5 parts of cellulase fermentations waste mycelias, 2 parts of natural pond slags, regulating water content behind the thorough mixing in fermentation vat is 80% (w/w), regulating pH is 5, handled 2 days under 40 ℃ of conditions, stirring is 5 times therebetween.
(2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with 100 parts of wherein cellulase fermentations waste mycelias, 8 parts in urea, and 9 parts of calcium superphosphate, 3 parts in Repone K, 5 parts of potassium primary phosphates, 18 parts of potassium breezes, initial water content 75%, pH 7.2.Above-mentioned substratum is standby after 30 minutes through 121 ℃ of sterilizations.
(3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering is with embodiment 1 step (3).
(4) solid state fermentation: Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water, insert in the solid-state fermentation culture medium according to following ratio.Bacillus circulans seed liquor 5, bacillusmusilaginosiengineering seed liquor 5, solid-state fermentation culture medium 250.The inoculation back mixes in fermentation vat, and controlled temperature is 36 ℃, and humidity is 95%, and fermentation time is 6 days, and stirring is 6 times therebetween.
(5) with embodiment 1 step (5).Living bacteria count is hundred million of 2-3/gram in the biological potassium fertilizer, and assorted bacterium rate is lower than 10%.
Embodiment 3
A kind of method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer, it comprises the steps: the pre-treatment of (1) natural pond slag: 1 part of cellulase fermentations waste mycelia, 6 parts of natural pond slags, regulating water content behind the thorough mixing in fermentation vat is 50% (w/w), regulating pH is 4.8, handled 5 days under 30 ℃ of conditions, stirring is 8 times therebetween.
(2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with 200 parts of wherein cellulase fermentations waste mycelias, 8 parts in urea, and 10 parts of calcium superphosphate, 3 parts in Repone K, 5 parts of potassium primary phosphates, 10 parts of potassium breezes, initial water content 80%, pH 8.Above-mentioned substratum is standby after 30 minutes through 121 ℃ of sterilizations.
(3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering is with embodiment 1 step (3).
(4) solid state fermentation.Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water respectively, insert in the solid-state fermentation culture medium according to following ratio.Bacillus circulans seed liquor 5, bacillusmusilaginosiengineering 10, solid-state fermentation culture medium 150.The inoculation back mixes in fermentation vat, and controlled temperature is 25 ℃, and humidity is 90%, and fermentation time is 8 days, and stirring is 10 times therebetween.
(5) with embodiment 1 step (5).Living bacteria count is hundred million of 2-2.5/gram in the biological potassium fertilizer, and assorted bacterium rate is lower than 15%.
Embodiment 4
A kind of method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer, it comprises the steps: the pre-treatment of (1) natural pond slag: 5 parts of cellulase fermentations waste mycelias, 2 parts of natural pond slags, regulating water content behind the thorough mixing in fermentation vat is 60% (w/w), regulating pH is 6, handled 3 days under 32 ℃ of conditions, stirring is 6 times therebetween.
(2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with 150 parts of wherein cellulase fermentations waste mycelias, 6 parts in urea, and 10 parts of calcium superphosphate, 0 part in Repone K, 2 parts of potassium primary phosphates, 20 parts of potassium breezes, initial water content 75%, pH 7.5.Above-mentioned substratum is standby after 30 minutes through 121 ℃ of sterilizations.
(3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering is with embodiment 1 step (3).
(4) solid state fermentation.Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water respectively, insert in the solid-state fermentation culture medium according to following ratio.Bacillus circulans seed liquor 10, bacillusmusilaginosiengineering 10, solid-state fermentation culture medium 120.The inoculation back mixes in fermentation vat, and controlled temperature is 37 ℃, and humidity is 90%, and fermentation time is 6 days, and stirring is 10 times therebetween.
(5) with embodiment 1 step (5).Living bacteria count is hundred million of 5-6/gram in the biological potassium fertilizer, and assorted bacterium rate is lower than 10%.
Claims (6)
1. method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer, it is characterized in that it is made up of following steps: the pre-treatment of (1) natural pond slag: adopt the cellulase fermentations waste mycelia to mix, the Mierocrystalline cellulose in the utilization cellulase activity hydrolysis natural pond slag wherein with the natural pond slag; (2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with wherein cellulase fermentations waste mycelia and urea, calcium superphosphate, and Repone K, potassium primary phosphate, the potassium breeze mixes, initial water content 60%-80%, pH 6-8; (3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering: be and shake bottle back and cultivate the acquisition shake-flask seed, again shake-flask seed is inoculated into and carries out amplification culture in the fermentor tank and obtain secondary seed with inoculating through the thalline of slant culture; (4) solid state fermentation is produced biological potassium fertilizer: Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water respectively, proportionally insert in the solid-state fermentation culture medium; The inoculation back mixes in fermentation vat, and fermentation time is 2-8 days; (5) drying.
2. the method for utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer according to claim 1 is characterized in that described cellulase waste mycelia is meant, the liquid fermentation ends of cellulase is after the resulting radicula byssoidea of filtration treatment.
3. the method for utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer according to claim 2 is characterized in that described fungi is meant that aspergillus, mould or wood are mould.
4. the method for utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer according to claim 1 is characterized in that described natural pond slag is meant the solid ingredient of the waste gained after solid-liquid separation that discharges in the marsh gas fermentation processes.
5. the method for utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer according to claim 1 is characterized in that described potassium breeze is meant one or more mixture of potassium felspar sand breeze, nepheline breeze, polyhalite breeze, alunite breeze.
6. method of utilizing cellulase fermentations waste mycelia and natural pond slag to produce biological potassium fertilizer, it is characterized in that it is made up of following steps: the pre-treatment of (1) natural pond slag: cellulase waste mycelia 1-5 part, natural pond slag 2-6 part, regulating water content behind the thorough mixing in fermentation vat is 50%-70%w/w, adjusting pH is 4-6, handled 1-5 days under 20 ℃ of-50 ℃ of conditions, stirring is 3-8 time therebetween;
(2) preparation of solid-state fermentation culture medium: the good natural pond slag of pre-treatment is together with wherein cellulase fermentations waste mycelia 100-200 part, urea 3-8 part, calcium superphosphate 5-10 part, Repone K 0-3 part, potassium primary phosphate 2-5 part, potassium breeze 10-20 part, initial water content 60%-80%, pH 6-8; Above-mentioned substratum is standby after 30 minutes through 121 ℃ of sterilizations;
(3) cultivation of Bacillus circulans and bacillusmusilaginosiengineering:
1. the cultivation of described Bacillus circulans:
Slant medium: peptone 1, extractum carnis 0.3, sodium-chlor 0.5, agar 2, water 100, pH 7.0; Thalline on the above-mentioned slant medium in incubator 32 ℃ be cultured to a large amount of preparations that are used for Bacillus circulans after the inclined-plane covers with;
Liquid nutrient medium: starch 5, sucrose 1, CaCO
31, FeCl
30.1, soybean cake powder 0.5, ammonium sulfate 2, potassium primary phosphate 1, sal epsom 0.5, tap water 1000, pH 7.2;
Shake-flask seed is cultivated: the shaking table culture condition is: 200 rev/mins of rotating speeds, temperature 35-40 ℃, incubation time 20-30 hour acquisition shake-flask seed;
Fermentor tank seed culture: in fermentor tank, insert shake-flask seed with the 10%v/v inoculum size, rotating speed 300-400 rev/min, culture temperature 35-40 ℃, dissolved oxygen is controlled at more than 20%, air flow be 1: 1 be the volume ratio of benchmark with the liquid amount, cultivate and obtained secondary seed in 15-28 hour;
2. the cultivation of described bacillusmusilaginosiengineering:
Slant medium: sucrose 10, SODIUM PHOSPHATE, MONOBASIC 2, sal epsom 0.5, ammonium sulfate 2, iron(ic) chloride 0.05, lime carbonate 1.0, yeast powder 2, agar 20 inclined-planes, water 1000,7.0,121 ℃ of sterilizations of pH 30 minutes; Thalline is used for liquid shaking bottle seed preparation being cultured in 32 ℃ of incubators on the above-mentioned slant medium after the inclined-plane covers with;
Liquid nutrient medium: starch 7, sucrose 1, CaCO
31, FeCl
30.1, soybean cake powder 0.5, ammonium sulfate 2, potassium primary phosphate 1, sal epsom 0.5, tap water 1000, pH 7.2;
The shake-flask seed culture condition is: 200 rev/mins of shaking speed, temperature 30-35 ℃, incubation time 10-15 hour acquisition shake-flask seed;
Fermentor tank seed culture: insert shake-flask seed to fermentor tank with the 10%v/v inoculum size, rotating speed 300-400 rev/min, culture temperature 30-35 ℃, dissolved oxygen is controlled at more than 20%, air flow is to be the volume ratio 1: 1 of benchmark with the liquid amount, cultivates to obtain secondary seed in 15-24 hour;
(4) solid state fermentation; Bacillus circulans liquid seeds liquid and bacillusmusilaginosiengineering seed liquor are diluted to 1,000,000,000/milliliter with sterilized water respectively, insert in the solid-state fermentation culture medium according to following ratio; The compound method of solid-state fermentation culture medium is seen step (2);
Bacillus circulans seed liquor 5-10, bacillusmusilaginosiengineering 5-10, solid-state fermentation culture medium 100-250; The inoculation back mixes in fermentation vat, and controlled temperature is 25-40 ℃, and humidity is 90%-95%, and fermentation time is 2-8 days, and stirring is 3-10 time therebetween;
(5) drying; Solid state fermentation is dried the gained fermentation fertilizer or is dried after finishing under 40-60 ℃ of condition, control its water content and can obtain the biological potassium fertilizer product at 20-30%.
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| CN102942391A (en) * | 2012-11-19 | 2013-02-27 | 黑龙江省科学院微生物研究所 | Method for producing actinomyces fertilizer by utilizing fermentation of biogas residues and biogas slurry solids |
| CN104291882A (en) * | 2013-07-15 | 2015-01-21 | 华东理工大学 | Method of preparing potassic fertilizer by decomposing non-water-soluble potassium mineral through penicillium fungus |
| CN108794104A (en) * | 2017-04-28 | 2018-11-13 | 唐健发 | A kind of ecological utilization method of potassium sewage |
| CN108911887A (en) * | 2018-08-31 | 2018-11-30 | 青岛华理琅新技术产业股份有限公司 | A kind of preparation method rich in ocean source Organic biological fertilizer |
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Cited By (4)
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| CN102942391A (en) * | 2012-11-19 | 2013-02-27 | 黑龙江省科学院微生物研究所 | Method for producing actinomyces fertilizer by utilizing fermentation of biogas residues and biogas slurry solids |
| CN104291882A (en) * | 2013-07-15 | 2015-01-21 | 华东理工大学 | Method of preparing potassic fertilizer by decomposing non-water-soluble potassium mineral through penicillium fungus |
| CN108794104A (en) * | 2017-04-28 | 2018-11-13 | 唐健发 | A kind of ecological utilization method of potassium sewage |
| CN108911887A (en) * | 2018-08-31 | 2018-11-30 | 青岛华理琅新技术产业股份有限公司 | A kind of preparation method rich in ocean source Organic biological fertilizer |
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