CN107164143B - Preparation method of cordyceps militaris three-penis wine - Google Patents
Preparation method of cordyceps militaris three-penis wine Download PDFInfo
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Abstract
The invention discloses a preparation method of cordyceps militaris three-penis wine, and belongs to the field of health-care wine. In the invention, part of the traditional Chinese medicines are fermented together with the glutinous rice, while other traditional Chinese medicines do not participate in fermentation; the fermentation bacteria are selected from three yeasts of alcohol yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 to be mixed with the distiller's yeast, so that the synergistic effect on the generation of esters and acid is realized, and the wine with high total ester content and high total acid content can be obtained; penis cervi, penis canitis, penis bovis seu Bubali and fructus amomi are selected to be fermented together with the glutinous rice, during the fermentation process, the components without medicinal effects are fermented, degraded and consumed, and the components with the medicinal effect of tonifying the kidney are well reserved and are more fully dissolved in the wine, so that the health-care efficacy of tonifying the kidney is enhanced; the wine provided by the invention has the advantages that the solubility of the active ingredients of the cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica which do not participate in fermentation is improved, and the kidney tonifying and health care effects are further improved.
Description
Technical Field
The invention relates to the field of health-care wine products, and particularly relates to a preparation method of cordyceps militaris three-penis wine.
Background
The medicinal liquor is a health-care product integrating traditional Chinese medicines for building up the body and wine, has stable medicinal properties, and is safe and effective. And because the alcohol is a good solvent, various active ingredients of the traditional Chinese medicine are easy to dissolve in the alcohol, and the efficacy of the traditional Chinese medicine is fully exerted by the aid of the alcohol and the medicinal effect of the liquor, so that the curative effect of the traditional Chinese medicine is improved.
The preparation method of the medicated liquor comprises soaking the Chinese medicinal materials in liquor, dissolving the effective components of the Chinese medicinal materials in the liquor after a certain period of time, and filtering to remove residues for drinking. According to the introduction of ancient and modern medical literature data and folk experience in China, the commonly used method for preparing the medicinal liquor mainly comprises a cold soaking method, a hot dipping method, a decocting method, a wine brewing method and the like.
The cold soaking method has very low utilization rate of medicinal materials, thereby causing waste; the utilization rate of the medicinal materials by a hot dipping method is improved, but the prepared medicinal liquor has poor taste; the decoction method sometimes destroys the property of Chinese herbs and is not suitable for herbs containing volatile effective components.
The brewing method comprises decocting the Chinese medicinal materials in water, filtering to remove residue, concentrating into medicinal juice, squeezing some medicinal materials to obtain juice, decocting Oryza Glutinosa to obtain cooked rice, mixing the medicinal juice, cooked Oryza Glutinosa and distiller's yeast, placing in a clean container, sealing, standing at a certain temperature for about 10 days while minimizing contact with air, maintaining at a certain temperature, fermenting, and filtering to obtain the final product. The existing wine brewing method is used for preparing medicinal liquor, all traditional Chinese medicines are fermented together without selection, so that active ingredients of some traditional Chinese medicines are damaged, and the obtained medicinal liquor is common in taste and relatively poor in texture by adopting distiller's yeast for fermentation.
The wine contains main components of ethanol, various substances for improving wine aroma, such as ester, aromatic compounds, ether, acid, ketone and the like, and different fermentation bacteria agents, and the types and the amounts of the substances for improving wine aroma in the obtained wine are different, so that the types and the amounts of effective components for dissolving traditional Chinese medicinal materials in the medicinal wine and the taste of the medicinal wine are different, and therefore, the problem to be solved is to obtain the fermentation bacteria agents capable of improving the wine aroma and the medicinal effect.
Disclosure of Invention
In order to make up for the defects of the prior art and solve the problems that the effective components of some medicines are damaged due to the fact that all traditional Chinese medicines are fermented by a wine brewing method in the prior art, and medicinal liquor obtained by fermenting distiller's yeast has common taste and relatively poor texture, the invention provides a preparation method of cordyceps militaris three-penis wine. The cordyceps militaris three-penis wine prepared by the invention has good taste and good drug effect.
The technical scheme of the invention is as follows:
a preparation method of cordyceps militaris three-penis wine comprises the following steps:
1) feeding 1000 parts of cooked glutinous rice, 20-80 parts of penis cervi, 10-30 parts of penis canitis, 10-30 parts of penis bovis seu Bubali and 5-25 parts of fructus amomi which are subjected to water absorption expansion and sterilization into a clean container;
2) adding 150 plus 250 portions of distiller's yeast and 50-150 portions of mixed brewing microbial inoculum, stirring uniformly, fermenting for 25-40 days at normal temperature; the mixed saccharomyces cerevisiae preparation comprises alcohol yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396;
3) filtering out solid residues, and sterilizing the filtrate;
4) adding 15-50 parts of cordyceps militaris, 30-60 parts of medlar, 30-60 parts of dried orange peel and 20-50 parts of angelica dahurica into the sterilized filtrate, soaking for 10-20 days at normal temperature, filtering out solid residues, and collecting the filtrate to obtain the cordyceps militaris three-penis wine.
As a preferred scheme, the preparation method of the mixed brewing microbial inoculum comprises the following steps:
a) respectively inoculating alcoholic yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 into sterilized wort triangular flask culture medium with sugar degree of 10-11 Balin, and shake culturing at 30-32 deg.C for 12-16 hr;
b) inoculating the bacterial liquid obtained in the step a) into the same solid culture medium according to the volume ratio of 1:0.3-0.8:0.3-0.8, and fermenting at normal temperature for 20-40 hours; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling;
c) vacuum drying at 30-35 deg.C, and vacuum packaging to obtain mixed brewing microbial inoculum when water content is less than 10%.
Further, in the step b), the alcohol yeast AS 2.300 bacterial liquid, the Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and the Issatchenkia orientalis NRRL Y-5396 bacterial liquid are inoculated according to the volume ratio of 1:0.4: 0.7.
Preferably, the concentration of the alcoholic yeast AS 2.300 bacterial liquid, the Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and the Issatchenkia orientalis NRRL Y-5396 bacterial liquid is 1 × 102-1×105cfu/mL。
As a preferred scheme, the cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica in the step 4) are crushed and then added into the sterilized filtrate.
Preferably, the penis cervi, the penis canitis, the penis bovis seu Bubali and the fructus amomi in the step 1) are respectively immersed in water, soaked for 3-8 hours, swollen by absorbing water and then sterilized.
The invention has the beneficial effects that:
according to the invention, penis cervi, penis canitis, penis bovis seu Bubali and fructus amomi are fermented together with glutinous rice, while cordyceps militaris, medlar, dried orange peel and radix angelicae do not participate in fermentation; the fermentation bacteria are selected from alcohol yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396, and mixed with distiller's yeast. On one hand, the fermentation strain selects three yeasts of alcohol yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 to be mixed with the distiller's yeast, so that the synergistic effect on the generation of esters and acid is realized, and the wine with high total ester content and high total acid content can be obtained; on the other hand, the deer penis, the dog penis, the bull penis and the amomum villosum are selected to be fermented together with the glutinous rice, during the fermentation process, the components without medicinal effects are fermented, degraded and consumed, and the components with the medicinal effects of tonifying the kidney are almost completely reserved and are more fully dissolved in the wine, so that the health-care efficacy of tonifying the kidney is enhanced; the cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica are not fermented but are cold-soaked in the wine, and the wine obtained by the invention has high total ester content and high total acid content, so that the solubility of the effective components in the cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica is improved, and the kidney tonifying and health care effects of the medicinal wine are further enhanced.
Detailed Description
The alcohol yeast AS 2.300 is a common microorganism strain, is carried in a catalogue of China general microorganism strain preservation management center and is in an open state, and scientific researchers can ask for the common microorganism strain preservation management center.
The Kluyveromyces marxianus standard strain AS2.1549 is a common microorganism strain, comes from the strain preservation center of Chinese academy of sciences, and can be requested by scientists from the strain preservation center of Chinese academy of sciences.
Issatchenkia orientalis NRRL Y-5396 is a common microorganism strain, is loaded in the American Ministry of agriculture strain collection center and is in an open state, and scientists can ask for the American Ministry of agriculture strain collection center.
Example 1
A preparation method of cordyceps militaris three-penis wine comprises the following steps:
1) feeding; putting 1000 parts of cooked glutinous rice, 50 parts of penis cervi, 20 parts of penis canitis, 20 parts of penis bovis seu Bubali and 15 parts of fructus amomi which are subjected to water swelling and sterilization into a clean container. Wherein the penis et testis Cervi, penis et testis Canis, testis Et penis bovis seu Bubali and fructus Amomi are respectively soaked in water for 4 hr, and then sterilized to obtain penis et testis Cervi, penis et testis Canis, testis Et penis bovis seu Bubali and fructus Amomi after swelling.
2) Adding 200 parts of distiller's yeast and 100 parts of mixed brewing microbial inoculum, stirring uniformly, and fermenting at normal temperature for 25-40 days;
the preparation method of the mixed brewing microbial inoculum comprises the following steps:
a) respectively inoculating alcoholic yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 into sterilized wort triangular flask culture medium with sugar degree of 10 Balin, and shake culturing at 30-32 deg.C for 15 hr; the obtained alcohol yeast AS 2.300 bacterial liquid, Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid have concentration of 1 × 103-1×104cfu/mL；
b) Inoculating the alcoholic yeast AS 2.300 bacterial liquid obtained in the step a), Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid in a volume ratio of 1:0.4:0.7 to the same solid culture medium, and fermenting at normal temperature for 30 hours; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling;
c) vacuum drying at 32 deg.C, and vacuum packaging when water content is less than 10% to obtain mixed brewing microbial inoculum.
3) Filtering out solid residues, and sterilizing the filtrate;
4) adding 30 parts of cordyceps militaris, 45 parts of medlar, 45 parts of dried orange peel and 35 parts of angelica dahurica into the sterilized filtrate, soaking for 15 days at normal temperature, filtering out solid residues, and collecting the filtrate to obtain the cordyceps militaris three-penis wine. Wherein the Cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica are crushed into 80 meshes.
Example 2
A preparation method of cordyceps militaris three-penis wine comprises the following steps:
1) feeding; putting 1000 parts of cooked glutinous rice, 60 parts of penis cervi, 15 parts of penis canis, 25 parts of penis bovis seu Bubali and 10 parts of fructus amomi which are subjected to water swelling and sterilization into a clean container. Wherein the penis et testis Cervi, penis et testis Canis, testis Et penis bovis seu Bubali and fructus Amomi are respectively soaked in water for 3 hr, and then are sterilized to obtain sterilized penis et testis Cervi, penis et testis Canis, testis Et penis bovis seu Bubali and fructus Amomi.
2) Adding 250 parts of distiller's yeast and 100 parts of mixed brewing microbial inoculum, stirring uniformly, and fermenting at normal temperature for 25-40 days;
the preparation method of the mixed brewing microbial inoculum comprises the following steps:
a) respectively inoculating alcoholic yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 into sterilized wort triangular flask culture medium with sugar degree of 10 Balin, and shake culturing at 30-32 deg.C for 16 hr; the obtained alcohol yeast AS 2.300 bacterial liquid, Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid have concentration of 1 × 103-1×105cfu/mL；
b) Inoculating the alcoholic yeast AS 2.300 bacterial liquid obtained in the step a), Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid in a volume ratio of 1:0.6:0.3 to the same solid culture medium, and fermenting at normal temperature for 30 hours; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling;
c) vacuum drying at 32 deg.C, and vacuum packaging when water content is less than 10% to obtain mixed brewing microbial inoculum.
3) Filtering out solid residues, and sterilizing the filtrate;
4) adding 40 parts of cordyceps militaris, 35 parts of medlar, 55 parts of dried orange peel and 45 parts of angelica dahurica into the sterilized filtrate, soaking for 14 days at normal temperature, filtering out solid residues, and collecting the filtrate to obtain the cordyceps militaris three-penis wine. Wherein the Cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica are crushed into 100 meshes.
Example 3
A preparation method of cordyceps militaris three-penis wine comprises the following steps:
1) feeding; putting 1000 parts of cooked glutinous rice, 30 parts of penis cervi, 25 parts of penis canitis, 15 parts of penis bovis seu Bubali and 25 parts of fructus amomi which are subjected to water swelling and sterilization into a clean container. Wherein the penis et testis Cervi, penis et testis Canis, testis Et penis bovis seu Bubali and fructus Amomi are respectively soaked in water for 6 hr, and then sterilized to obtain penis et testis Cervi, penis et testis Canis, testis Et penis bovis seu Bubali and fructus Amomi after swelling.
2) Adding 160 parts of distiller's yeast and 120 parts of mixed brewing microbial inoculum, stirring uniformly, and fermenting for 30 days at normal temperature;
the preparation method of the mixed brewing microbial inoculum comprises the following steps:
a) respectively inoculating alcoholic yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 into sterilized wort triangular flask culture medium with sugar degree of 10 Balin, and shake culturing at 30-32 deg.C for 12 hr; the obtained alcohol yeast AS 2.300 bacterial liquid, Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid have concentration of 1 × 102-1×103cfu/mL；
b) Inoculating the alcoholic yeast AS 2.300 bacterial liquid obtained in the step a), Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid in a volume ratio of 1:0.3:0.7 to the same solid culture medium, and fermenting at normal temperature for 30 hours; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling;
c) vacuum drying at 32 deg.C, and vacuum packaging when water content is less than 10% to obtain mixed brewing microbial inoculum.
3) Filtering out solid residues, and sterilizing the filtrate;
4) adding 20 parts of cordyceps militaris, 55 parts of medlar, 35 parts of dried orange peel and 25 parts of angelica dahurica into the sterilized filtrate, soaking for 12 days at normal temperature, filtering out solid residues, and collecting the filtrate to obtain the cordyceps militaris three-penis wine. Wherein the Cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica are crushed into 50 meshes.
Comparative example 1
Compared with the embodiment 1, the preparation method only mixing the brewing microbial inoculum is different.
The preparation method of the mixed brewing microbial inoculum in the comparative example comprises the following steps:
a) respectively inoculating alcohol yeast AS 2.300 and Kluyveromyces marxianus standard strain AS2.1549 in sterilized triangular flask culture medium of wort with sugar degree of 10 barlin, and shake culturing at 30-32 deg.C for 15 hr; the obtained alcohol yeast AS 2.300 bacterial liquid and Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid have concentration of 1 × 103-1×104cfu/mL；
b) Inoculating the alcohol yeast AS 2.300 bacterial liquid obtained in the step a) and the Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid into the same solid culture medium according to the volume ratio of 1:1.1, and fermenting for 30 hours at normal temperature; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling;
c) vacuum drying at 32 deg.C, and vacuum packaging when water content is less than 10% to obtain mixed brewing microbial inoculum.
Comparative example 2
Compared with the embodiment 1, the preparation method only mixing the brewing microbial inoculum is different.
The preparation method of the mixed brewing microbial inoculum in the comparative example comprises the following steps:
a) respectively inoculating alcoholic yeast AS 2.300 and Issatchenkia orientalis NRRL Y-5396 into sterilized triangular flask culture medium of malt wort with sugar degree of 10 Balin, and shake culturing at 30-32 deg.C for 15 hr; the obtained alcoholic yeast AS 2.300 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid have concentration of 1 × 103-1×104cfu/mL；
b) Inoculating the alcoholic yeast AS 2.300 bacterial liquid obtained in the step a) and the Issatchenkia orientalis NRRL Y-5396 bacterial liquid in a same solid culture medium according to the volume ratio of 1:1.1, and fermenting for 30 hours at normal temperature; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling;
c) vacuum drying at 32 deg.C, and vacuum packaging when water content is less than 10% to obtain mixed brewing microbial inoculum.
Comparative example 3
Compared with the embodiment 1, the preparation method only mixing the brewing microbial inoculum is different.
The preparation method of the mixed brewing microbial inoculum in the comparative example comprises the following steps:
a) respectively inoculating Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 into sterilized wort triangular flask culture medium with sugar degree of 10 Balin, and shake culturing at 30-32 deg.C for 15 hr; the obtained Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and Issatchenkia orientalis NRRL Y-5396 bacterial liquid have concentration of 1 × 103-1×104cfu/mL；
b) Inoculating the Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and the Issatchenkia orientalis NRRL Y-5396 bacterial liquid obtained in the step a) into the same solid culture medium according to the volume ratio of 0.4: 1.7, and fermenting for 30 hours at normal temperature; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling;
c) vacuum drying at 32 deg.C, and vacuum packaging when water content is less than 10% to obtain mixed brewing microbial inoculum.
Comparative example 4
50 parts of penis cervi, 20 parts of penis canitis, 20 parts of penis bovis seu Bubali, 15 parts of fructus amomi, 30 parts of cordyceps militaris, 45 parts of fructus lycii, 45 parts of pericarpium citri reticulatae and 35 parts of radix angelicae, and soaking in white wine for 30 days.
Comparative example 5
50 parts of penis cervi, 20 parts of penis canitis, 20 parts of penis bovis seu Bubali, 15 parts of fructus amomi, 30 parts of cordyceps militaris, 45 parts of fructus lycii, 45 parts of pericarpium citri reticulatae and 35 parts of radix angelicae are decocted by adding water, dregs are filtered to obtain medicine juice, the medicine juice is uniformly mixed with glutinous rice and distiller's yeast, the mixture is placed in a clean container and fermented, dregs are filtered after the fermentation is finished, and the filtrate is the medicinal liquor.
The results of the tests on the total ester content and total acid content of the sterile filtrate obtained in the respective examples and comparative step 3) are shown in table 1:
table 1:
as can be seen from Table 1, the mixed brewing microbial inoculum of the invention
The preparation method of the mixed brewing microbial inoculum comprises the following steps: the alcohol yeast AS 2.300, the Kluyveromyces marxianus standard strain AS2.1549 and the Issatchenkia orientalis NRRL Y-5396 have obvious synergistic effect on ester-producing and acid-producing; the effect of the invention cannot be achieved by replacing three strains with any two combinations thereof.
Mouse sperm impact test
120 clean male mice are taken and randomly divided into three groups, the mice are respectively fed with the medicinal liquor and the physiological saline of example 1, comparative example 4 and comparative example 5, 0.1mL/10g of the mice are re-filled into the stomach, the mice are killed after cervical vertebra removal once a day for 15 days continuously on the 16 th day, epididymis tissues are rapidly picked up, the mice are weighed and then placed into a mortar for grinding, the mice are diluted by 15 times by using wilted cell liquid, the number of sperms is counted, the activity and the survival rate of the sperms are observed, and the number of the sperms is converted into ten thousand/10 mg of epididymis tissues, and the result is shown in a table 2.
TABLE 2
As can be seen from Table 2, the present invention has very good kidney tonifying effect, which is significantly superior to comparative examples 4 and 5.
Compared with the cordyceps militaris three-penis wine prepared by the traditional method, the cordyceps militaris three-penis wine prepared by the method has better kidney tonifying and health care effects.
Claims (6)
1. A preparation method of cordyceps militaris three-penis wine is characterized by comprising the following steps: 1) feeding 1000 parts of cooked glutinous rice, 20-80 parts of penis cervi, 10-30 parts of penis canitis, 10-30 parts of penis bovis seu Bubali and 5-25 parts of fructus amomi which are subjected to water absorption expansion and sterilization into a clean container; 2) adding 150 plus 250 portions of distiller's yeast and 50-150 portions of mixed brewing microbial inoculum, stirring uniformly, fermenting for 25-40 days at normal temperature; the mixed saccharomyces cerevisiae preparation comprises alcohol yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396; 3) filtering out solid residues, and sterilizing the filtrate; 4) adding 15-50 parts of cordyceps militaris, 30-60 parts of medlar, 30-60 parts of dried orange peel and 20-50 parts of angelica dahurica into the sterilized filtrate, soaking for 10-20 days at normal temperature, filtering out solid residues, and collecting the filtrate to obtain the cordyceps militaris three-penis wine.
2. The preparation method of the cordyceps militaris three-penis wine as claimed in claim 1, wherein the preparation method of the mixed brewing microbial inoculum comprises the steps of: a) respectively inoculating alcoholic yeast AS 2.300, Kluyveromyces marxianus standard strain AS2.1549 and Issatchenkia orientalis NRRL Y-5396 into sterilized wort triangular flask culture medium with sugar degree of 10-11 Balin, and shake culturing at 30-32 deg.C for 12-16 hr; b) inoculating the bacterial liquid obtained in the step a) into the same solid culture medium according to the volume ratio of 1:0.3-0.8:0.3-0.8, and fermenting at normal temperature for 20-40 hours; the solid culture medium is prepared from 80% of bran and 20% of wheat flour by moistening with water, steaming, and cooling; c) vacuum drying at 30-35 deg.C, and vacuum packaging to obtain mixed brewing microbial inoculum when water content is less than 10%.
3. The preparation method of the cordyceps militaris three-animal penes wine as claimed in claim 2, which is characterized in that: in the step b), the alcohol yeast AS 2.300 bacterial liquid, the Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and the Issatchenkia orientalis NRRL Y-5396 bacterial liquid are inoculated according to the volume ratio of 1:0.4: 0.7.
4. The preparation method of the cordyceps militaris three-animal penes wine as claimed in claim 2, which is characterized in that: the concentration of the alcohol yeast AS 2.300 bacterial liquid, the Kluyveromyces marxianus standard strain AS2.1549 bacterial liquid and the Issatchenkia orientalis NRRL Y-5396 bacterial liquid is 1 multiplied by 102-1×105cfu/mL。
5. The preparation method of the cordyceps militaris three-animal penes wine as claimed in claim 1, which is characterized in that: and 4) crushing the cordyceps militaris, the medlar, the dried orange peel and the angelica dahurica, and adding the crushed cordyceps militaris, the crushed medlar, the crushed dried orange peel and the crushed angelica dahurica into the sterilized filtrate.
6. The preparation method of the cordyceps militaris three-penis wine as claimed in claim 1 or 2, which is characterized in that: step 1), respectively adding water to the penis cervi, the penis canitis, the penis bovis seu Bubali and the fructus amomi for immersing for 3-8 hours, absorbing water for swelling, and then sterilizing.
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| CN102586125A (en) * | 2012-02-21 | 2012-07-18 | 湖北白云边酒业股份有限公司 | Highly ester producing issatchenkia orientalis yeast and composition as well as application thereof |
| CN102703288A (en) * | 2012-05-23 | 2012-10-03 | 冷振民 | Cordyceps sinensis three-animal-penis wine and method for producing cordyceps sinensis three-animal-penis wine |
| CN103333811A (en) * | 2013-06-21 | 2013-10-02 | 武汉轻工大学 | Kluyveromyces marxianus as well as composition and application thereof |
| CN103725575A (en) * | 2014-01-22 | 2014-04-16 | 雷正新 | Bagged preparation of health care wine and preparation method thereof |
| CN104531477A (en) * | 2014-12-23 | 2015-04-22 | 重庆工商大学 | Peony kidney-nourishing wine and preparation method thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586125A (en) * | 2012-02-21 | 2012-07-18 | 湖北白云边酒业股份有限公司 | Highly ester producing issatchenkia orientalis yeast and composition as well as application thereof |
| CN102703288A (en) * | 2012-05-23 | 2012-10-03 | 冷振民 | Cordyceps sinensis three-animal-penis wine and method for producing cordyceps sinensis three-animal-penis wine |
| CN103333811A (en) * | 2013-06-21 | 2013-10-02 | 武汉轻工大学 | Kluyveromyces marxianus as well as composition and application thereof |
| CN103725575A (en) * | 2014-01-22 | 2014-04-16 | 雷正新 | Bagged preparation of health care wine and preparation method thereof |
| CN104531477A (en) * | 2014-12-23 | 2015-04-22 | 重庆工商大学 | Peony kidney-nourishing wine and preparation method thereof |
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