CN107129052A - A kind of biological flocculation for aquiculture fast culture process - Google Patents

A kind of biological flocculation for aquiculture fast culture process Download PDF

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Publication number
CN107129052A
CN107129052A CN201710257812.8A CN201710257812A CN107129052A CN 107129052 A CN107129052 A CN 107129052A CN 201710257812 A CN201710257812 A CN 201710257812A CN 107129052 A CN107129052 A CN 107129052A
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China
Prior art keywords
floc sedimentation
biological flocculation
culture
cultural method
daily
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CN201710257812.8A
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Inventor
刘青松
张家松
李华
董宏标
段亚飞
王涛
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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Priority to CN201710257812.8A priority Critical patent/CN107129052A/en
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/14NH3-N

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a kind of fast culture process of biological flocculation for aquiculture, its step is:Biological flocculation is concentrated, after vexed exposure, and feed is fed daily, adds carbon source, regulation temperature and pH, biological flocculation is arranged daily, set floc sedimentation residence time SRT, and the floc sedimentation discharged daily and the floc sedimentation of culture are merged and diluted, expands culture.Using the biological flocculation of the cultural method culture, aquaculture discharge water environment can be more rapidly adapted to, ammonia-nitrogen content relatively low in water environment is maintained, so that with efficient pollutant place to go performance.

Description

A kind of biological flocculation for aquiculture fast culture process
Technical field
The invention belongs to aquaculture water treatment field, more particularly to aquaculture process discharge residual bait excrement is in situ or different Position biologic treating technique.
Background technology
With being continuously increased for world population, protein requirement is just in sustainable growth.Aquatic products are sought as human protein Foster important sources, global demand amount is also increasingly improved.It is that cultivation scale constantly expands that such a demand, which increases incident, Proposals of Water Resources is caused acid test.Traditional extensive style pond is big to water demand, and with dirts such as substantial amounts of nitrogen phosphorus Thing discharge is contaminated, causes surrounding body environmental degradation, is developed against in culture fishery green and healthy.On the other hand, by a large amount of The extensive style pond culture mode for changing water significantly increases the probability of external source pathogenic infection aquaculture organism, so as to add cultivation Family carries out the frequency of disease prevention and cure using chemical agents such as antibiotic, and a large amount of use of these medicaments not only makes germ, parasite Etc. developing immunity to drugs, and water environment biological community structure stability can be damaged, and trigger food-safety problem.
These urgent problems to be solved promote many experts and scholars to start to probe into a kind of sustainability cultivation water processing mode, To promote culture fishery to develop in a healthy way.In recent years, the green cultivation technology based on biological flocculation is arisen at the historic moment.Biological flocculation skill Art(BFT)With regulation and control water quality, raising efficiency of feed utilization, strengthen the effect of biological prevention and control, and strong temperature in breeding process can be reduced The burst size of room gaseous nitrous oxide, causes the extensive concern of domestic and international related researcher and cultural technique personnel.
However, biological flocculation is generally used as inoculation floc sedimentation by adding biological agent and cultivated at present, or directly foster Grow and brown sugar and feed culture indigenous microorganism are added in pond to form floc sedimentation, cultivation cycle is long, and early stage floc sedimentation disposal ability has Limit, easily because the nitrogen pollutant load of cultivation discharge exceedes the load that can bear of floc sedimentation and caused by ammonia nitrogen, nitrite nitrogen etc. Accumulation, causes water quality deterioration.
For above-mentioned situation, the present invention proposes a kind of biological flocculation fast culture mode, and because of condition of culture and actual water Produce cultivation situation similar, aquaculture discharge water environment can be rapidly adapted to by cultivating floc sedimentation, so as to be gone with efficient pollutant Except performance.
The content of the invention
It is an object of the invention to disclose a kind of fast culture process of biological flocculation for aquiculture.
The technical solution used in the present invention is:
A kind of fast culture process of biological flocculation for aquiculture, its step is:Biological flocculation is concentrated, after vexed exposure, daily Feed is fed, carbon source, regulation temperature and pH is added, biological flocculation is arranged daily, floc sedimentation residence time SRT is set, will be discharged daily Floc sedimentation and culture floc sedimentation merge dilution, expand culture.
It is preferred that, selected carbon source is brown sugar.
It is preferred that, need to maintain C/N during addition carbon source 15~20.
It is preferred that, vexed quick-fried condition is:One day 2 cycle of operation, each cycle is aerated 8~11h, 0.5~2h of anaerobism, precipitation 0.5~1h.
It is preferred that, dissolved oxygen DO maintains 3.5~7mg/L in incubation.
It is preferred that, pH maintains 7~8.5 in incubation, and temperature maintains 25~35 DEG C.
It is preferred that, set and maintain the residence time SRT of floc sedimentation to be 10~30d.
It is preferred that, the biological flocculation and the biological flocculation of culture discharged daily merge dilution, it is ensured that biological flocculation concentration exists 2000~6000mg/L;Every SRT incubation time, expand culture once.
A kind of application of biological flocculation cultural method in aquaculture water treatment field.
The beneficial effects of the invention are as follows:The cultural method of the biological flocculation, can more rapidly adapt to aquaculture discharge water Environment, maintains ammonia-nitrogen content relatively low in water environment, so that with efficient contaminant-removal properties.
Embodiment
It is as described below, it is only the embodiment of the present invention, protection scope of the present invention is not limited to this.
Embodiment 1:
4520mg/L biological flocculation 5L is taken from Environment of Litopenaeus vannamei Low biological flocculation cultivating system, progress is vexed quick-fried, and vexed quick-fried condition is:One Its 2 cycle of operation, each cycle is aerated 8h, anaerobism 2h, precipitates 1h.After vexed exposure 48h, feed 2.5g~3.1g is fed daily, it is red Sugared addition is 2.3~2.8g, and it is that 15, DO maintains 3.8~5mg/L to maintain C/N, and pH maintains 7.2~8.1, and temperature is maintained 25 DEG C, row's biological flocculation 1/20, sets the floc sedimentation residence time daily(SRT)For 20d, after 20d, by the floc sedimentation discharged daily and training Foster floc sedimentation merges, while being diluted to 5760mg/L, biological flocculation volume expands culture to 20L from 5L;30d, floc sedimentation volume 25L is extended to, floc sedimentation concentration is 5760mg/L, and total floc sedimentation amount increases 122.75g.The floc sedimentation increment of this method is greatly increased, And required reactor volume is small, floor space is saved.After 60d, floc sedimentation volume expansion culture to 100L, concentration 5260mg/L, often Day, feed treating capacity increased to 43g, and according to water-quality determination result, ammonia nitrogen maintains below 0.05mg/L, and nitrite nitrogen is maintained In below 0.2mg/L, cultivation water demand is met.
Embodiment 2:
The biological flocculation 5L that concentration is 3600mg/L is taken out from Tilapia mossambica biological flocculation cultivating system, vexed quick-fried, vexed quick-fried condition is carried out It is:One day 2 cycle of operation, each cycle is aerated 11h, anaerobism 0.5h, precipitates 0.5h.After vexed exposure 48h, feed 1.5g is fed daily ~2.5g, brown sugar addition is 1.6~2.6g, and it is that 20, DO maintains 3.5~4.5mg/L to maintain C/N, pH maintains 7.1~ 7.9, temperature maintains 35 DEG C, daily row's biological flocculation 1/20, sets the floc sedimentation residence time(SRT), will be daily after 20d for 20d The floc sedimentation of discharge and the floc sedimentation of culture merge, while being diluted to 5260mg/L, biological flocculation volume expands culture to 15L from 5L; After 60d, floc sedimentation volume is extended to 88L, concentration 5040mg/L, and daily feed treating capacity increases to 31g, according to water-quality determination knot Really, ammonia nitrogen maintains below 0.05mg/L, and nitrite nitrogen maintains below 0.2mg/L, meets cultivation water demand.

Claims (9)

1. a kind of fast culture process of biological flocculation for aquiculture, its step is:Biological flocculation is concentrated, after vexed exposure, often Day feeds feed, adds carbon source, regulation temperature and pH, biological flocculation is arranged daily, set floc sedimentation residence time SRT, will arrange daily The floc sedimentation and the floc sedimentation of culture gone out merges dilution, expands culture.
2. cultural method according to claim 1, it is characterised in that the carbon source is brown sugar.
3. cultural method according to claim 1, it is characterised in that C/N maintains 15~20 during addition carbon source.
4. cultural method according to claim 1, it is characterised in that vexed quick-fried condition is:One day 2 cycle of operation, each Cycle is aerated 8~11h, 0.5~2h of Anaerobic culturel, precipitates 0.5~1h.
5. cultural method according to claim 1, it is characterised in that in incubation dissolved oxygen DO maintain 3.5~ 7mg/L。
6. cultural method according to claim 1, it is characterised in that regulation pH value is 7~8.5, regulation temperature is 25~ 35℃。
7. cultural method according to claim 1, it is characterised in that floc sedimentation residence time SRT is 10~30d.
8. cultural method according to claim 1, it is characterised in that the floc sedimentation and the floc sedimentation of culture discharged daily merge dilute Release, ensure biological flocculation concentration in 2000~6000mg/L after dilution;Every SRT incubation time, expand culture once.
9. application of any cultural methods of claim 1-8 in aquaculture water treatment field.
CN201710257812.8A 2017-04-19 2017-04-19 A kind of biological flocculation for aquiculture fast culture process Pending CN107129052A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109231503A (en) * 2018-11-15 2019-01-18 中国水产科学研究院珠江水产研究所 A kind of biological membrane anaerobic denitrification reinforcing carbon source screening technique for pond wastewater treatment
CN110255715A (en) * 2019-06-20 2019-09-20 中国水产科学研究院渔业机械仪器研究所 The method for cultivating diatom advantage limnobios floc sedimentation using cultivation tail water

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102487879A (en) * 2011-12-12 2012-06-13 中国水产科学研究院黑龙江水产研究所 Method for facilitating formation of microbial floccules in freshwater aquiculture pond
CN103627655A (en) * 2013-10-28 2014-03-12 中国水产科学研究院珠江水产研究所 Indoor culture method of biological flocs
KR20170030682A (en) * 2015-09-09 2017-03-20 이태영 Aquaculture tank with biofloc and system and method using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102487879A (en) * 2011-12-12 2012-06-13 中国水产科学研究院黑龙江水产研究所 Method for facilitating formation of microbial floccules in freshwater aquiculture pond
CN103627655A (en) * 2013-10-28 2014-03-12 中国水产科学研究院珠江水产研究所 Indoor culture method of biological flocs
KR20170030682A (en) * 2015-09-09 2017-03-20 이태영 Aquaculture tank with biofloc and system and method using the same

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Title
姚妙兰: "生物絮凝技术应用于卤虫养殖的初步研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109231503A (en) * 2018-11-15 2019-01-18 中国水产科学研究院珠江水产研究所 A kind of biological membrane anaerobic denitrification reinforcing carbon source screening technique for pond wastewater treatment
CN110255715A (en) * 2019-06-20 2019-09-20 中国水产科学研究院渔业机械仪器研究所 The method for cultivating diatom advantage limnobios floc sedimentation using cultivation tail water
CN110255715B (en) * 2019-06-20 2021-08-20 中国水产科学研究院渔业机械仪器研究所 Method for cultivating diatom dominant freshwater biological flocs by using culture tail water

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Application publication date: 20170905