CN107125243B - Sterilant for inhibiting insect reproduction and preparation method thereof - Google Patents
Sterilant for inhibiting insect reproduction and preparation method thereof Download PDFInfo
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- CN107125243B CN107125243B CN201710411633.5A CN201710411633A CN107125243B CN 107125243 B CN107125243 B CN 107125243B CN 201710411633 A CN201710411633 A CN 201710411633A CN 107125243 B CN107125243 B CN 107125243B
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- gossypol
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- oligosaccharin
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- acetone
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- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 26
- 241000238631 Hexapoda Species 0.000 title abstract description 22
- 238000002360 preparation method Methods 0.000 title abstract description 14
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 claims abstract description 210
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 claims abstract description 105
- 229930000755 gossypol Natural products 0.000 claims abstract description 105
- 229950005277 gossypol Drugs 0.000 claims abstract description 105
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 100
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000012153 distilled water Substances 0.000 claims abstract description 50
- 239000000877 Sex Attractant Substances 0.000 claims abstract description 49
- 239000000203 mixture Substances 0.000 claims abstract description 39
- 238000003756 stirring Methods 0.000 claims abstract description 36
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims abstract description 27
- 239000002994 raw material Substances 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 238000005303 weighing Methods 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims description 9
- 241000255967 Helicoverpa zea Species 0.000 claims description 6
- 230000035755 proliferation Effects 0.000 claims 4
- 230000000694 effects Effects 0.000 abstract description 14
- 238000002474 experimental method Methods 0.000 abstract description 13
- 239000003814 drug Substances 0.000 abstract description 8
- 229940079593 drug Drugs 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 229920000742 Cotton Polymers 0.000 description 8
- 241001147381 Helicoverpa armigera Species 0.000 description 8
- 230000036512 infertility Effects 0.000 description 6
- 230000012447 hatching Effects 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 241001124076 Aphididae Species 0.000 description 3
- 241000238876 Acari Species 0.000 description 2
- 241000256248 Spodoptera Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 239000005886 Chlorantraniliprole Substances 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- NIOHNDKHQHVLKA-UHFFFAOYSA-N acetic acid;7-(8-formyl-1,6,7-trihydroxy-3-methyl-5-propan-2-ylnaphthalen-2-yl)-2,3,8-trihydroxy-6-methyl-4-propan-2-ylnaphthalene-1-carbaldehyde Chemical compound CC(O)=O.CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 NIOHNDKHQHVLKA-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- PSOVNZZNOMJUBI-UHFFFAOYSA-N chlorantraniliprole Chemical group CNC(=O)C1=CC(Cl)=CC(C)=C1NC(=O)C1=CC(Br)=NN1C1=NC=CC=C1Cl PSOVNZZNOMJUBI-UHFFFAOYSA-N 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an sterilant for inhibiting insect reproduction and a preparation method thereof, wherein the drug composition comprises the following raw materials in parts by weight: 1 to 20 parts of gossypol, 0.5 to 10 parts of amino-oligosaccharin, 0.1 to 5 parts of sex attractant, 0.1 to 5 parts of acetone and 1500 to 5000 parts of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product. Experiments show that after the gossypol and the auxiliary material amino-oligosaccharin are used according to a certain dosage, the sterile effect is obviously improved under the same dosage, and the amino-oligosaccharin can effectively reduce the dosage of the gossypol and has the synergistic effect.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to an sterilant for inhibiting insect reproduction and a preparation method thereof.
Background
Cotton bollworms belong to the genus spodoptera of the family spodoptera and are widely distributed in cotton areas of yellow river basin and cotton areas of Yangtze river basin. When bollworms are harmful to cotton, the larvae are mainly used for eating the buds, flowers and bolls of the cotton. The buds are eaten by the moths, the buds are opened and yellow, and the buds fall off after 2-3 d; after the stigma and anther of the flower are damaged, the flower cannot pollinate and bell; after the green bell is eaten into a cavity, pathogen infection is often induced to cause rotting bell, and the larvae eat tender tips and tender leaves of cotton to form holes and nicks, so that headless cotton is caused, and normal development of the cotton is affected. Cotton bollworms affect major crops such as wheat, corn, soybean, peanut, tomato, and the like, in addition to cotton.
With respect to the control of cotton bollworms, the traditional methods include agricultural control, biological control, physical control and chemical control. The common medicines are Du Bangkang wide and Fugoer medicines. Du Bangkang the main active ingredient is chlorantraniliprole, the principle of the pesticide is to prevent the absorption of calcium ions in pests, accelerate the release of calcium by the pests, prevent the pests from moving, and starve the pests after feeding, but act slowly, and the pesticide effect can last for 30 days due to the systemic conduction. However, the use of any chemical has a degree of pollution to the soil and the environment.
Disclosure of Invention
The invention aims to provide an sterilant for inhibiting insect reproduction, which has the characteristics of simple composition, novel formula, low cost and good effect.
The aim of the invention is realized by the following technical scheme:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 1 to 20 parts of gossypol, 0.5 to 10 parts of amino-oligosaccharin, 0.1 to 5 parts of sex attractant, 0.1 to 5 parts of acetone and 1500 to 5000 parts of distilled water.
Preferably, the weight parts of the raw materials are as follows: 3-16 parts of gossypol, 1-8 parts of amino-oligosaccharin, 0.5-4 parts of sex attractant, 0.5-4 parts of acetone and 2000-4000 parts of distilled water.
Preferably, the weight parts of the raw materials are as follows: 5-12 parts of gossypol, 3-6 parts of amino-oligosaccharin, 1-3 parts of sex attractant, 1-3 parts of acetone and 3000-3500 parts of distilled water.
Preferably, the weight parts of the raw materials are as follows: 1 part of gossypol, 0.5 part of amino-oligosaccharin, 0.1 part of sex attractant, 0.1 part of acetone and 2500 parts of distilled water.
A method of preparing the sterilant of any preceding claim, wherein: the method comprises the following steps:
s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby;
s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use;
s3: dissolving gossypol with acetone to obtain gossypol solution;
s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution;
s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
The beneficial effects of the invention are as follows: the sterilant of the invention can effectively reduce the egg drop of cotton bollworms, effectively reduce the number of larvae, and further improve the crop yield. In addition, the sterilant of the invention can effectively prevent and treat aphids and mites from breeding. The sterilant of the invention has the advantages of simple composition, novel formula, low cost, good control effect, environmental friendliness and the like, and is suitable for industrial production.
Detailed Description
The technical scheme of the present invention is described in further detail below with reference to specific embodiments, but the scope of the present invention is not limited to the following description.
Example 1:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 1g of gossypol, 0.5g of amino-oligosaccharin, 0.1g of sex attractant, 0.1g of acetone and 1500g of distilled water.
Example 2:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 20g of gossypol, 10g of amino-oligosaccharin, 5g of sex attractant, 5g of acetone and 5000g of distilled water.
Example 3:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 3g of gossypol, 1g of amino-oligosaccharin, 0.5g of sex attractant, 0.5g of acetone and 2000g of distilled water.
Example 4:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 16g of gossypol, 8g of amino-oligosaccharin, 4g of sex attractant, 4g of acetone and 4000g of distilled water.
Example 5:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: gossypol 5g, amino oligosaccharin 3g, sex attractant 1g, acetone 1g and distilled water 3000g.
Example 6:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 12g of gossypol, 6g of amino-oligosaccharin, 3g of sex attractant, 3g of acetone and 3500g of distilled water.
Example 7:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: gossypol 5g, amino oligosaccharin 1g, sex attractant 0.1g, acetone 0.1g, distilled water 2500g.
Example 8:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 1g of gossypol, 0.5g of amino-oligosaccharin, 0.1g of sex attractant, 0.1g of acetone and 1500g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 9:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 20g of gossypol, 10g of amino-oligosaccharin, 5g of sex attractant, 5g of acetone and 5000g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 10:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 3g of gossypol, 1g of amino-oligosaccharin, 0.5g of sex attractant, 0.5g of acetone and 2000g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 11:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 16g of gossypol, 8g of amino-oligosaccharin, 4g of sex attractant, 4g of acetone and 4000g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 12:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 3g of gossypol, 3g of amino-oligosaccharin, 0.1g of sex attractant, 0.1g of acetone and 3000g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 13:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 12g of gossypol, 6g of amino-oligosaccharin, 3g of sex attractant, 3g of acetone and 3500g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 14:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 1.5g of gossypol, 0.5g of amino-oligosaccharin, 0.1g of sex attractant, 0.1g of acetone and 2500g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 15:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: gossypol 5g, amino oligosaccharin 1g, sex attractant 0.1g, acetone 0.1g, distilled water 2500g. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
Example 16:
an sterilant for inhibiting insect reproduction comprises the following raw materials in parts by weight: 10g of gossypol, 1g of amino-oligosaccharin, 0.1g of sex attractant, 0.1g of acetone and 2500g of distilled water. The preparation method comprises the following steps: s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby; s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use; s3: dissolving gossypol with acetone to obtain gossypol solution; s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution; s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
The beneficial effects of the invention are verified by specific experimental study:
earlier studies found that when the concentration of gossypol was 800mg/kg, the egg mass was reduced by 97.6% compared with the control, the hatchability was 0, the sterility effect was 100%, and other treatments greater than 800mg/kg could achieve 100%. The following experiments continued with intensive studies based on earlier findings.
1. Impact of gossypol on control of bollworms.
The gossypol is selected as 95% acetic acid gossypol raw powder, after the gossypol is dissolved by acetone, the gossypol is diluted into gossypol liquid with different concentrations by 0.8% syrup, and the amino-oligosaccharin is directly added into distilled water to be diluted into amino-oligosaccharin solution with different concentrations. The experiments were divided into 8 test groups, group A (gossypol concentration 400 mg/kg), group B (gossypol concentration 600 mg/kg), group C (gossypol concentration 800 mg/kg), group D (gossypol concentration 1000 mg/kg), group E (amino-oligosaccharin 1000 mg/kg), group F (gossypol concentration 400mg/kg + amino-oligosaccharin 400 mg/kg), group G (gossypol concentration 600mg/kg + amino-oligosaccharin 600 mg/kg), group H (gossypol concentration 800mg/kg + amino-oligosaccharin 800 mg/kg), control group I (8% sugar water). Cotton bollworms were harvested from the eastern cotton-boring area and adults were fed in 8 cages of 30cm×30 cm. Each cage is respectively fed with the liquid medicine.
The result shows that when the concentration of gossypol is 400mg/kg, the egg mass is reduced by 88.46% compared with the control group I, and the hatching rate is 13.48%; when the concentration of gossypol is 600mg/kg, the egg mass is reduced by 91.18% compared with the control group I, and the hatching rate is 13.24%; when the concentration of gossypol is 800mg/kg, the egg mass is reduced by 95.33% compared with the control group I, the hatching rate is 0, and the sterile effect reaches 100%. When the concentration of gossypol is 1000mg/kg, the sterility effect reaches 100%. The concentration of the amino-oligosaccharin is 1000mg/kg, and the sterile effect is avoided. After the gossypol concentration is 400mg/kg and the amino-oligosaccharin concentration is 400mg/kg, the egg mass is reduced by 91.30 percent compared with the control group I, and the hatching rate is 14.93 percent; the egg laying amount is reduced by 97.41 percent after the gossypol concentration is 600mg/kg and the amino-oligosaccharin concentration is 600mg/kg, and the hatching sterile effect reaches 100 percent; after the gossypol concentration is 800mg/kg and the amino-oligosaccharin concentration is 800mg/kg, the spawning quantity is 0, and the sterility effect reaches 100%; the results are shown in Table 1.
TABLE 1 determination of the sterility Effect of different concentrations of liquid medicine on Heliothis armigera
The experimental result shows that after the gossypol is mixed with the auxiliary material amino-oligosaccharin for use, the sterile effect is obviously improved under the same dosage, and the amino-oligosaccharin can effectively reduce the dosage of the gossypol and has the synergistic effect.
2. The effect of different dosage ratios of gossypol on preventing and controlling cotton bollworms.
The experiments were divided into 5 test groups, group a (gossypol concentration: amino-oligosaccharin=1:1, see example 12), group B (gossypol concentration: amino-oligosaccharin=3:1, see example 14), group C (gossypol concentration: amino-oligosaccharin=5:1, see example 15), group D (gossypol concentration: amino-oligosaccharin=10:1, see example 16), control group E (8% sugar water). The experiment A, the experiment B, the experiment C and the experiment D are uniformly dosed according to the dosage of 600mg/kg of the drug concentration through the dosage proportion. Cotton bollworms were harvested from the eastern cotton-boring area and adults were fed in 5 cages of 30cm×30 cm. Each cage is respectively fed with the liquid medicine.
The result shows that when the concentration of gossypol is 600mg/kg and the concentration of amino-oligosaccharin is 600mg/kg, the spawning quantity is 0, and the sterility effect reaches 100%; the spawning amount of the experiment A, the experiment B and the experiment C is 0, the sterile effect can reach 100%, and the experiment D reaches 95.68%. From the perspective of saving medicines and pharmaceutic adjuvants, the experimental group C has the best compatibility because the application of the adjuvants is the least, but still has the same therapeutic effect.
In addition, in aphid and mite sterility experiments, the sterilant can effectively inhibit the propagation of aphids and mites.
Claims (5)
1. A method of inhibiting the proliferation of cotton bollworms, comprising: the sterilant is prepared from the following raw materials in parts by weight: 1 to 20 parts of gossypol, 0.5 to 10 parts of amino-oligosaccharin, 0.1 to 5 parts of sex attractant, 0.1 to 5 parts of acetone and 1500 to 5000 parts of distilled water; wherein, the concentration of the gossypol is 600mg/kg or 800mg/kg, and the weight ratio of the gossypol to the amino-oligosaccharin is 1:1, 3:1 or 5:1.
2. A method of inhibiting the proliferation of cotton bollworms according to claim 1, wherein: the sterilant comprises the following raw materials in parts by weight: 3-16 parts of gossypol, 1-8 parts of amino-oligosaccharin, 0.5-4 parts of sex attractant, 0.5-4 parts of acetone and 2000-4000 parts of distilled water.
3. A method of inhibiting the proliferation of cotton bollworms according to claim 1, wherein: the sterilant comprises the following raw materials in parts by weight: 5-12 parts of gossypol, 3-6 parts of amino-oligosaccharin, 1-3 parts of sex attractant, 1-3 parts of acetone and 3000-3500 parts of distilled water.
4. A method of inhibiting the proliferation of cotton bollworms according to claim 1, wherein: the sterilant comprises the following raw materials in parts by weight: 5 parts of gossypol, 1 part of amino-oligosaccharin, 0.1 part of sex attractant, 0.1 part of acetone and 2500 parts of distilled water.
5. A method of preparing the sterilant according to any one of claims 1 to 4, wherein: the method comprises the following steps:
s1: weighing gossypol, amino oligosaccharin, sex attractant, acetone and distilled water according to the weight ratio for standby;
s2: mixing amino oligosaccharin and sex attractant to obtain a mixture for later use;
s3: dissolving gossypol with acetone to obtain gossypol solution;
s4: mixing the mixture obtained in the step S2 with the gossypol dissolving solution obtained in the step S3, and then pouring the mixture into distilled water for fully stirring to obtain a stirring solution;
s5: and (3) subpackaging and sterilizing the stirring liquid obtained in the step (S4) to obtain a finished product.
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