CN107125243A - A kind of apholate for suppressing insect reproduction and preparation method thereof - Google Patents
A kind of apholate for suppressing insect reproduction and preparation method thereof Download PDFInfo
- Publication number
- CN107125243A CN107125243A CN201710411633.5A CN201710411633A CN107125243A CN 107125243 A CN107125243 A CN 107125243A CN 201710411633 A CN201710411633 A CN 201710411633A CN 107125243 A CN107125243 A CN 107125243A
- Authority
- CN
- China
- Prior art keywords
- gossypol
- amino
- oligosaccharide
- gyplure
- distilled water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- PXZAWHSJYIECNQ-UHFFFAOYSA-N apholate Chemical compound C1CN1P1(N2CC2)=NP(N2CC2)(N2CC2)=NP(N2CC2)(N2CC2)=N1 PXZAWHSJYIECNQ-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 241000238631 Hexapoda Species 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title abstract description 14
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 claims abstract description 196
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 100
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 claims abstract description 98
- 229930000755 gossypol Natural products 0.000 claims abstract description 98
- 229950005277 gossypol Drugs 0.000 claims abstract description 98
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 60
- 239000012153 distilled water Substances 0.000 claims abstract description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- YKPQYKBIAFGWBT-KAMYIIQDSA-N [(Z)-18-hydroxyoctadec-9-en-7-yl] acetate Chemical compound CCCCCCC(C\C=C/CCCCCCCCO)OC(C)=O YKPQYKBIAFGWBT-KAMYIIQDSA-N 0.000 claims abstract description 49
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 239000002994 raw material Substances 0.000 claims abstract description 25
- 238000003756 stirring Methods 0.000 claims abstract description 23
- 239000006166 lysate Substances 0.000 claims abstract description 22
- 239000000203 mixture Substances 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 19
- 239000000463 material Substances 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 16
- 229920000742 Cotton Polymers 0.000 description 14
- 238000012360 testing method Methods 0.000 description 10
- 241000255967 Helicoverpa zea Species 0.000 description 8
- -1 amino Oligosaccharide Chemical class 0.000 description 8
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 6
- 108010000912 Egg Proteins Proteins 0.000 description 6
- 210000004681 ovum Anatomy 0.000 description 6
- 239000003814 drug Substances 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 208000000509 infertility Diseases 0.000 description 4
- 230000036512 infertility Effects 0.000 description 4
- 231100000535 infertility Toxicity 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 239000006188 syrup Substances 0.000 description 3
- 235000020357 syrup Nutrition 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001600407 Aphis <genus> Species 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000255990 Helicoverpa Species 0.000 description 1
- 241000256257 Heliothis Species 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- LJKDPUCDUDLUOM-UHFFFAOYSA-N [3,8-diacetyloxy-1-formyl-6-methyl-4-propan-2-yl-7-(1,6,7-triacetyloxy-8-formyl-3-methyl-5-propan-2-ylnaphthalen-2-yl)naphthalen-2-yl] acetate Chemical compound CC(C)C1=C(OC(C)=O)C(OC(C)=O)=C(C=O)C2=C(OC(C)=O)C(C=3C(OC(C)=O)=C4C(C=O)=C(OC(C)=O)C(OC(C)=O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 LJKDPUCDUDLUOM-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229940003871 calcium ion Drugs 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- PSOVNZZNOMJUBI-UHFFFAOYSA-N chlorantraniliprole Chemical compound CNC(=O)C1=CC(Cl)=CC(C)=C1NC(=O)C1=CC(Br)=NN1C1=NC=CC=C1Cl PSOVNZZNOMJUBI-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
Abstract
The invention discloses a kind of apholate for suppressing insect reproduction and preparation method thereof, the pharmaceutical composition is made up of the raw material of following ratio of weight and number：1~20 part of gossypol, 0.5~10 part of amino-oligosaccharide, 0.1~5 part of gyplure, 0.1~5 part of acetone, 1,500 5000 parts of distilled water.Preparation method comprises the following steps：S1：Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are weighed by weight, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, mixture is obtained, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The mixture that S2 is walked is mixed with the gossypol lysate that S3 is walked, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed, sterilized, is got product.After being shown experimentally that gossypol and auxiliary material amino-oligosaccharide by doses compatibility use, sterile effect is significantly improved under identical dosage, shows that amino-oligosaccharide can effectively reduce the usage amount of gossypol, both have the effect of Synergistic.
Description
Technical field
The invention belongs to biological technical field, it is related to a kind of apholate for suppressing insect reproduction and preparation method thereof.
Background technology
Genus heliothis is distributed widely in Huanghe valley cotton region, Yangtze river basin cotton in Noctuidae Helicoverpa borer pest
Area.Bollworm cause harm cotton when, mainly eaten into larva and eat the flower bud of cotton, flower, bell.Flower bud opens jaundice, 2-3d by bract after moth food
After come off；After colored column cap and flower pesticide are killed, it is impossible to pollination knot bell；Blue or green bell is eaten into behind cavity, often induces infection process, causes
Rotten bell, larva also eats the tender point of harmful cotton and tender leaf, forms hole and incises, cause cotton without a head, influences the normal development of cotton.Cotton
Before earworm is except influence cotton, the staple crops such as wheat, corn and soybean, peanut and tomato can be also influenceed.
On the preventing and treating of bollworm, conventional method has cultural control, biological control, physical control and chemical prevention.It is conventional
Medicine has Du Pont's health wide, the medicament such as Fu Ge.The wide active ingredient of Du Pont's health is Rynaxypyr, and insecticide principle is to prevent insect
Internal Calcium-ion absorption, accelerates the release of insect calcium, insect can not be moved, it is impossible to died of hunger after taking food, but effect come it is slow, because of it
There is uptake and translocation effect, lasting medicine was up to 30 days.But, the use of any chemicals has certain journey to soil and environment
The pollution of degree.
The content of the invention
It is an object of the present invention to provide one kind composition simply, the infertility for being used to suppress insect reproduction of novel formula
Agent, the apholate has the characteristics of cost is low, effect is good.
The purpose of the present invention is achieved through the following technical solutions：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：1~20 part of gossypol,
0.5~10 part of amino-oligosaccharide, 0.1~5 part of gyplure, 0.1~5 part of acetone, 1500-5000 parts of distilled water.
Preferably, the ratio of weight and number of described each raw material is：3~16 parts of gossypol, 1~8 part of amino-oligosaccharide, gyplure
0.5~4 part, 0.5~4 part of acetone, 2000-4000 parts of distilled water.
Preferably, the ratio of weight and number of described each raw material is：5~12 parts of gossypol, 3~6 parts of amino-oligosaccharide, gyplure
1~3 part, 1~3 part of acetone, 3000-3500 parts of distilled water.
Preferably, the ratio of weight and number of described each raw material is：1 part of gossypol, 0.5 part of amino-oligosaccharide, gyplure 0.1
Part, 0.1 part of acetone, 2500 parts of distilled water.
A kind of method for preparing the apholate described in above-mentioned any one claim, it is characterised in that：This method includes
Following steps：
S1：Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are weighed by weight, it is standby；
S2：Amino-oligosaccharide and gyplure are mixed, mixture is obtained, it is standby；
S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；
S4：The mixture that S2 is walked is mixed with the gossypol lysate that S3 is walked, and is then poured into distilled water and is sufficiently stirred for again, obtains
To stirring liquid；
S5：The S4 stirring liquid walked is dispensed, sterilized, is got product.
The beneficial effects of the invention are as follows：What apholate of the present invention can effectively reduce bollworm falls ovum amount, effectively reduces larva
Quantity, and then improve crop yield.In addition, apholate of the present invention can also effectively be prevented eliminating aphis, the breeding of mite class.The present invention is not
Educate agent and constitute simple, novel formula, have the advantages that with low cost, prevention effect is good, environmentally friendly, and be applicable to industry
Metaplasia is produced.
Embodiment
Technical scheme is described in further detail with reference to specific embodiment, but protection scope of the present invention is not
It is confined to as described below.
Embodiment 1：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 1g, amino is few
Sugared element 0.5g, gyplure 0.1g, acetone 0.1g, distilled water 1500g.
Embodiment 2：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 20g, amino
Oligosaccharide 10g, gyplure 5g, acetone 5g, distilled water 5000g.
Embodiment 3：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 3g, amino is few
Sugared element 1g, gyplure 0.5g, acetone 0.5g, distilled water 2000g.
Embodiment 4：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 16g, amino
Oligosaccharide 8g, gyplure 4g, acetone 4g, distilled water 4000g.
Embodiment 5：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 5g, amino is few
Sugared element 3g, gyplure 1g, acetone 1g, distilled water 3000g.
Embodiment 6：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 12g, amino
Oligosaccharide 6g, gyplure 3g, acetone 3g, distilled water 3500g.
Embodiment 7：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 5g, amino is few
Sugared element 1g, gyplure 0.1g, acetone 0.1g, distilled water 2500g.
Embodiment 8：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 1g, amino is few
Sugared element 0.5g, gyplure 0.1g, acetone 0.1g, distilled water 1500g.Specific preparation method comprises the following steps：S1：By weight
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are weighed, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, obtained
Mixture, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The cotton that the mixture that S2 is walked is walked with S3
Phenol lysate is mixed, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed,
Sterilizing, gets product.
Embodiment 9：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 20g, amino
Oligosaccharide 10g, gyplure 5g, acetone 5g, distilled water 5000g.Specific preparation method comprises the following steps：S1：By weight title
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are taken, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, mixed
Compound, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The gossypol that the mixture that S2 is walked is walked with S3
Lysate is mixed, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed, gone out
Bacterium, gets product.
Embodiment 10：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 3g, amino is few
Sugared element 1g, gyplure 0.5g, acetone 0.5g, distilled water 2000g.Specific preparation method comprises the following steps：S1：By weight title
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are taken, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, mixed
Compound, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The gossypol that the mixture that S2 is walked is walked with S3
Lysate is mixed, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed, gone out
Bacterium, gets product.
Embodiment 11：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 16g, amino
Oligosaccharide 8g, gyplure 4g, acetone 4g, distilled water 4000g.Specific preparation method comprises the following steps：S1：Weigh by weight
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, mixed
Thing, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The mixture that S2 is walked and the gossypol that S3 is walked are molten
Liquid mixing is solved, then pours into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed, gone out
Bacterium, gets product.
Embodiment 12：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 3g, amino is few
Sugared element 3g, gyplure 0.1g, acetone 0.1g, distilled water 3000g.Specific preparation method comprises the following steps：S1：By weight title
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are taken, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, mixed
Compound, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The gossypol that the mixture that S2 is walked is walked with S3
Lysate is mixed, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed, gone out
Bacterium, gets product.
Embodiment 13：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 12g, amino
Oligosaccharide 6g, gyplure 3g, acetone 3g, distilled water 3500g.Specific preparation method comprises the following steps：S1：Weigh by weight
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, mixed
Thing, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The mixture that S2 is walked and the gossypol that S3 is walked are molten
Liquid mixing is solved, then pours into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed, gone out
Bacterium, gets product.
Embodiment 14：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 1.5g, amino
Oligosaccharide 0.5g, gyplure 0.1g, acetone 0.1g, distilled water 2500g.Specific preparation method comprises the following steps：S1：By weight
It is standby than weighing gossypol, amino-oligosaccharide, gyplure, acetone and distilled water；S2：Amino-oligosaccharide and gyplure are mixed, obtained
It is standby to mixture；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The mixture that S2 is walked is walked with S3
Gossypol lysate is mixed, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is divided
Dress, sterilizing, get product.
Embodiment 15：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 5g, amino is few
Sugared element 1g, gyplure 0.1g, acetone 0.1g, distilled water 2500g.Specific preparation method comprises the following steps：S1：By weight title
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are taken, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, mixed
Compound, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The gossypol that the mixture that S2 is walked is walked with S3
Lysate is mixed, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed, gone out
Bacterium, gets product.
Embodiment 16：
A kind of apholate for suppressing insect reproduction, it is made up of the raw material of following ratio of weight and number：Gossypol 10g, amino
Oligosaccharide 1g, gyplure 0.1g, acetone 0.1g, distilled water 2500g.Specific preparation method comprises the following steps：S1：By weight
Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are weighed, it is standby；S2：Amino-oligosaccharide and gyplure are mixed, obtained
Mixture, it is standby；S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；S4：The cotton that the mixture that S2 is walked is walked with S3
Phenol lysate is mixed, and is then poured into distilled water and is sufficiently stirred for again, obtains stirring liquid；S5：The S4 stirring liquid walked is dispensed,
Sterilizing, gets product.
Beneficial effects of the present invention are verified below by specific experimental study：
Early-stage Study finds that, when gossypol concentration is 800mg/kg, ovum amount reduces 97.6% than control, and incubation rate is 0, no
Effect is educated up to 100%, and other processing more than 800mg/kg can reach 100% effect.Following experiments are according to early-stage Study
It was found that proceeding further investigation.
1. influence of the gossypol to preventing and treating bollworm.
It is the former powder of 95% gossypol acetic acid from gossypol, gossypol is diluted to difference first with after acetone solution, then with 0.8% syrup
The gossypol liquid of concentration, amino-oligosaccharide is directly added into distilled water diluting into the amino-oligosaccharide solution of various concentrations.By experiment point
For 8 test groups, experiment A groups (gossypol concentration 400mg/kg), experiment B groups (gossypol concentration 600mg/kg) test C group (gossypols
Concentration 800mg/kg), experiment D groups (gossypol concentration 1000mg/kg), experiment E groups (amino-oligosaccharide 1000mg/kg) test F groups
(gossypol concentration 400mg/kg+ amino-oligosaccharide 400mg/kg), tests G group (gossypol concentration 600mg/kg+ amino-oligosaccharides
600mg/kg), experiment H groups (gossypol concentration 800mg/kg+ amino-oligosaccharide 800mg/kg), control I groups (8% syrup).Cotton boll
Worm picks up from Distributions in Liaocheng of Shandong Province cotton region and adult is put in man-made feeds raising in 30cm × 30cm × 30cm 8 cages.Each cage
Above-mentioned decoction is fed respectively.
As a result show, when gossypol concentration is 400mg/kg, ovum amount reduces 88.46% than control I groups, and incubation rate is
13.48%；When gossypol concentration is 600mg/kg, ovum amount reduces 91.18% than control I groups, and incubation rate is 13.24%；Work as cotton
When phenol concentration is 800mg/kg, ovum amount is than control I groups reduction 95.33%, and incubation rate is 0, and sterile effect is up to 100%.Work as gossypol
When concentration is 1000mg/kg, sterile effect is up to 100%.Amino-oligosaccharide concentration is 1000mg/kg, without sterile effect.Gossypol is dense
Spend after 400mg/kg+ amino-oligosaccharides 400mg/kg mixing, ovum amount reduces 91.30% than control I groups, and incubation rate is 14.93%；
Egg laying amount reduces 97.41% after gossypol concentration 600mg/kg+ amino-oligosaccharides 600mg/kg mixing, and the sterile effect of hatching reaches
100%；After gossypol concentration 800mg/kg+ amino-oligosaccharides 800mg/kg mixing, egg laying amount is 0, and sterile effect is up to 100%；Knot
Fruit is shown in Table 1.
Sterile effect measuring of the various concentrations decoction of table 1 to bollworm
It is above-mentioned test result indicates that, after gossypol and auxiliary material amino-oligosaccharide are used in mixed way, the infertility effect under identical dosage
Fruit significantly improves, and shows that amino-oligosaccharide can effectively reduce the usage amount of gossypol, both have the effect of Synergistic.
2. gossypol various dose is with the influence for comparing preventing and treating bollworm.
Experiment is divided into 5 test groups, A group (gossypol concentration is tested：Amino-oligosaccharide=1:1, see embodiment 12), experiment
B groups (gossypol concentration：Amino-oligosaccharide=3:1, see embodiment 14), test C group (gossypol concentration：Amino-oligosaccharide=5:1, see
Embodiment 15), test D group (gossypol concentration：Amino-oligosaccharide=10:1, see embodiment 16), control E groups (8% syrup).Experiment
A groups, test B groups, and experiment C groups and experiment D groups are unified to be given by drug concentration 600mg/kg dosage by above-mentioned dosage ratio
Medicine.Bollworm picks up from Distributions in Liaocheng of Shandong Province cotton region and adult is put in man-made feeds raising in 30cm × 30cm × 30cm 5 cages.Often
Individual cage feeds above-mentioned decoction respectively.
As a result show, after gossypol concentration 600mg/kg+ amino-oligosaccharides 600mg/kg is mixed, egg laying amount is 0, infertility effect
Really up to 100%；A groups are tested, experiment B groups, experiment C group egg laying amounts are 0, sterile effect is up to 100%, and experiment D groups reach
95.68%.Said from medicament-saving and pharmaceutic adjuvant angle, test C groups, auxiliary material uses minimum, but still with identical treatment
Effect, therefore experiment C groups are optimal compatibility groups.
In addition, in aphid, mite class infertility it was found that, apholate of the present invention can also effectively suppress aphid, mite class it is numerous
Grow.
Claims (5)
1. a kind of apholate for suppressing insect reproduction, it is characterised in that：It is made up of the raw material of following ratio of weight and number：Gossypol
1~20 part, 0.5~10 part of amino-oligosaccharide, 0.1~5 part of gyplure, 0.1~5 part of acetone, 1500-5000 parts of distilled water.
2. a kind of apholate for suppressing insect reproduction according to claim 1, it is characterised in that：The weight of described each raw material
Measuring portion rate is：3~16 parts of gossypol, 1~8 part of amino-oligosaccharide, 0.5~4 part of gyplure, 0.5~4 part of acetone, distilled water
2000-4000 parts.
3. a kind of apholate for suppressing insect reproduction according to claim 1, it is characterised in that：The weight of described each raw material
Measuring portion rate is：5~12 parts of gossypol, 3~6 parts of amino-oligosaccharide, 1~3 part of gyplure, 1~3 part of acetone, distilled water 3000-
3500 parts.
4. a kind of apholate for suppressing insect reproduction according to claim 1, it is characterised in that：The weight of described each raw material
Measuring portion rate is：5 parts of gossypol, 1 part of amino-oligosaccharide, 0.1 part of gyplure, 0.1 part of acetone, 2500 parts of distilled water.
5. a kind of method for preparing the apholate in Claims 1 to 4 described in any one claim, it is characterised in that：Should
Method comprises the following steps：
S1：Gossypol, amino-oligosaccharide, gyplure, acetone and distilled water are weighed by weight, it is standby；
S2：Amino-oligosaccharide and gyplure are mixed, mixture is obtained, it is standby；
S3：Acetone solution gossypol is used, gossypol lysate is obtained, it is standby；
S4：The mixture that S2 is walked is mixed with the gossypol lysate that S3 is walked, and is then poured into distilled water and is sufficiently stirred for again, is stirred
Mix liquid；
S5：The S4 stirring liquid walked is dispensed, sterilized, is got product.
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| CN116784334A (en) * | 2023-06-26 | 2023-09-22 | 山东省农业科学院 | Composition containing gossypol for killing borer |
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