CN107125025A - A kind of cordyceps militaris cultivation method - Google Patents
A kind of cordyceps militaris cultivation method Download PDFInfo
- Publication number
- CN107125025A CN107125025A CN201710366919.6A CN201710366919A CN107125025A CN 107125025 A CN107125025 A CN 107125025A CN 201710366919 A CN201710366919 A CN 201710366919A CN 107125025 A CN107125025 A CN 107125025A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- weight
- parts
- temperature
- mycelia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of cordyceps militaris cultivation method, it includes following step：（1）After the 0.03 0.04 special anti-fouling agents of parts by weight cordyceps sinensis are substantially dissolved in the water of 50 DEG C of 1.5 parts by weight, in the rice or broken corn that pour into 1 parts by weight, culture medium is made after high-temperature sterilization；（2）Cordceps militaris original seed strain is accessed in above-mentioned culture medium；（3）Temperature keeps 15 18 DEG C of shading cultures, when mycelial growth to culture medium 1/2 2/3, temperature can be risen to mycelia after 20 DEG C, 20 days can send out full, when occurring mycelia projection on culture medium, 10 15 DEG C of thermal stimulation is given, and increases light stimulation annesl, has turned color within 7 10 days, when stroma length is to 1 centimetre, fully ventilation, temperature keeps 18 22 DEG C, relative air humidity 80 90%；（4）Stroma can be harvested when being in Chinese red or bar-shaped crocus.Cordyceps militaris cultivation method of the invention is pollution-free, cost is low, simplicity is convenient.
Description
Technical field
The present invention relates to plant cultivation technology, and in particular to a kind of cordyceps militaris cultivation method.
Background technology
Cordceps militaris is also known as Cordyceps militaris, belongs to ascomycetous ergot Zoopagales, Clavicipitaceae.Wild resource is increasingly deficient in recent years
It is weary, and the Cordceps militaris of artificial cultivation is very popular with good medicinal health care function.And existing Cordceps militaris is planted at present
Easily pollution, cost are high mostly for training technology.
The content of the invention
For above-mentioned problems of the prior art, the present invention provides a kind of cordyceps militaris cultivation method.The north of the present invention
Cordyceps sinensis cultural method is pollution-free, cost is low, method is easy.
Specifically, the present invention provides a kind of cordyceps militaris cultivation method, and it comprises the steps：
（1）After the special anti-fouling agent of 0.03-0.04 parts by weight cordyceps sinensis is substantially dissolved in the water of 50 DEG C of 1.5 parts by weight, 1 weight is poured into
In the rice or broken corn of measuring part, culture medium is made after high-temperature sterilization；
（2）Cordceps militaris original seed strain is accessed in above-mentioned culture medium；
（3）Temperature keeps 15-18 DEG C of shading culture, and when mycelial growth is to culture medium 1/2-2/3, temperature can be risen to 20 DEG C,
Mycelia can send out full after 20 days, when occurring mycelia projection on culture medium, give 10-15 DEG C of thermal stimulation, and increase illumination thorn
Swash annesl, turned color within 7-10 days, when stroma length is to 1 centimetre, fully ventilation, temperature keeps 18-22 DEG C, relative air humidity 80-
90%；
（4）Stroma can be harvested when being in Chinese red or bar-shaped crocus.
Wherein, the special anti-fouling agent of the cordyceps sinensis is 0.035 parts by weight.
In addition, the Cordceps militaris original seed strain is slant tube kind or liquid spawn.
In addition, the special anti-fouling agent of cordyceps sinensis is the special specific anti-fouling agent of edible mushroom.
Cordceps militaris is the fungi with higher nourishing and medical value, wide market.Reasonably base carbon-nitrogen ratio is
The requirement of artificial cultivation Cordceps militaris.Carbon source and nitrogen source necessary to being provided for growth as culture medium by the use of rice,
And the addition of anti-fouling agent then solves the problems, such as living contaminants in Edible Fungi.
Embodiment
Technical scheme is described in detail with reference to embodiments, but the present invention is not limited to this.
Embodiment
Raw material requirement：
1st, from mycelia is pure white, strong adaptability, see annesl after light, go out the stable fast growing and high yield good quality strain of careless fast, character.
2nd, gone mouldy from fresh nothing, free of contamination rice.
3rd, from more than 0.05 millimeter of thickness, specification is 30 centimetres of 15 cm x, and thin and thick is uniform, strong without run-down, toughness
High-quality polypropylene plastics pocket is cultivating container.
4th, it is loss of preventing and remedying pollution, the special anti-fouling agent of cordyceps sinensis, which is selected, integrates nutrition, edible mushroom that is antifouling, encouraging effect
Special specific anti-fouling agent.
Cultural method：
1st, culture medium prescription：1 kilogram of rice, 35 grams of the special anti-fouling agent of cordyceps sinensis, 1.5 kilograms of water.
2nd, compound method：Rice is distributed into polybag by 50 grams every bag.50 DEG C of hot water is poured into clean basin, will be eaten
Be put into the special specific anti-fouling agent of bacterium in hot water, stir to be completely dissolved nutrient solution.By material-water ratio（That is rice and nutrient solution
Amount ratio）1 gram:1.5 milliliters weigh nutrient solution and pour into the polybag for installing rice.With plastic ties by each plastics after having dispensed
Together with bag middle part is respectively pricked with oral area.
3rd, sterilize：Add suitable quantity of water in autoclave, culture pocket is put into autoclave, be warming up to 100 DEG C, kept for 1 hour.
Take out, transfer room is moved into after cooling.With the closed sterilization of high-quality aerial fog disinfectant or conventional method 30 minutes, you can wearing sterilization clothing
Clothes enter transfer room inoculation.
4th, it is inoculated with：It should be shut up the door carefully rapidly into transfer room（Prevent the air containing miscellaneous bacteria from entering）.Outside inoculating tool, strain
Wall, operating personnel's both hands etc., are sterilized with 75% alcohol wipe or dipping.
The expansion that turns of test tube original seed is cultivated：The strain 2-3 blocks for taking beans size after test tube plug with the inoculation hook of sterilization are taken out,
On access culture basal plane, sack is slightly tightened.As conditions permit can suitably increase inoculum concentration, comparatively fast grown with sharp mycelia.Connect
Sack is slightly tightened by former tying method after finishing, moves into and is cultivated through sterilizing in the culturing room with insect-prevention treatment.
5th, cultivate：Temperature is initially kept into 15-18 DEG C of shading culture, can be by when mycelial growth is to culture medium 1/2-2/3
Temperature rises to 20 DEG C, and mycelia can send out purseful within 20 days.After a few days, when mycelia projection occurs in culture medium top, 10-15 DEG C is given
Thermal stimulation, and increase light stimulation annesl.Turned within 7-10 days after color to culturing room sterilize 30 minutes, then solution go cultivate pocket
Bag, straight-tube shape is converted into hand by middle part jag, and space is provided for stroma growth.It is logical to cultivating bag in time when stroma length is to 1 centimetre
Gas, temperature keeps 18-22 DEG C, relative air humidity 80-90%.
6th, harvest：When stroma is in Chinese red or crocus bar-shaped not regrowth, open sack pocket knife or scissors by its from
Base portion is adopted down, is put into clean utensil and is dried or dry in time, is not exposed to the sun in order to avoid fading.It is stored in after drying in sealed plastic bag
Or sell.
Claims (4)
1. a kind of cordyceps militaris cultivation method, it is characterised in that comprise the steps：
（1）After the special anti-fouling agent of 0.03-0.04 parts by weight cordyceps sinensis is substantially dissolved in the water of 50 DEG C of 1.5 parts by weight, 1 weight is poured into
In the rice or broken corn of measuring part, culture medium is made after high-temperature sterilization；
（2）Cordceps militaris original seed strain is accessed in above-mentioned culture medium；
（3）Temperature keeps 15-18 DEG C of shading culture, and when mycelial growth is to culture medium 1/2-2/3, temperature can be risen to 20 DEG C,
Mycelia can send out full after 20 days, when occurring mycelia projection on culture medium, give 10-15 DEG C of thermal stimulation, and increase illumination thorn
Swash annesl, turned color within 7-10 days, when stroma length is to 1 centimetre, fully ventilation, temperature keeps 18-22 DEG C, relative air humidity 80-
90%；
（4）Stroma can be harvested when being in Chinese red or bar-shaped crocus.
2. cultural method according to claim 1, it is characterised in that the special anti-fouling agent of cordyceps sinensis is 0.035 parts by weight.
3. cultural method according to claim 1, it is characterised in that the Cordceps militaris original seed strain be slant tube kind or
Liquid spawn.
4. cultural method according to claim 1, it is characterised in that the special anti-fouling agent of cordyceps sinensis is the special spy of edible mushroom
Imitate anti-fouling agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366919.6A CN107125025A (en) | 2017-05-23 | 2017-05-23 | A kind of cordyceps militaris cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366919.6A CN107125025A (en) | 2017-05-23 | 2017-05-23 | A kind of cordyceps militaris cultivation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107125025A true CN107125025A (en) | 2017-09-05 |
Family
ID=59733290
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710366919.6A Pending CN107125025A (en) | 2017-05-23 | 2017-05-23 | A kind of cordyceps militaris cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107125025A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114175984A (en) * | 2022-01-18 | 2022-03-15 | 宜兴乾元黑色食品科技有限公司 | Method for inducing leaves of Vaccinium bracteatum plants to rapidly turn red through temperature difference treatment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101173221A (en) * | 2007-11-09 | 2008-05-07 | 乔善成 | Wheat cultivation Cordyceps militaris method |
| CN104472208A (en) * | 2014-11-11 | 2015-04-01 | 冀宏 | Liquid culture method for cordyceps militaris stroma |
| CN106520565A (en) * | 2016-10-24 | 2017-03-22 | 蔡凤文 | Method for preparing cordyceps mycelium through glutinous rice |
| CN106613359A (en) * | 2016-12-30 | 2017-05-10 | 临沂大学 | Cordyceps liquid cultivation medium bottle cultivation mushroom fruiting cultivation method |
-
2017
- 2017-05-23 CN CN201710366919.6A patent/CN107125025A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101173221A (en) * | 2007-11-09 | 2008-05-07 | 乔善成 | Wheat cultivation Cordyceps militaris method |
| CN104472208A (en) * | 2014-11-11 | 2015-04-01 | 冀宏 | Liquid culture method for cordyceps militaris stroma |
| CN106520565A (en) * | 2016-10-24 | 2017-03-22 | 蔡凤文 | Method for preparing cordyceps mycelium through glutinous rice |
| CN106613359A (en) * | 2016-12-30 | 2017-05-10 | 临沂大学 | Cordyceps liquid cultivation medium bottle cultivation mushroom fruiting cultivation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114175984A (en) * | 2022-01-18 | 2022-03-15 | 宜兴乾元黑色食品科技有限公司 | Method for inducing leaves of Vaccinium bracteatum plants to rapidly turn red through temperature difference treatment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105532266B (en) | A kind of collybia albuminosa cultural method | |
| CN105940960B (en) | A kind of soil covering culture method of interplanting ganoderma lucidum in tea gardens | |
| CN101720627A (en) | Method for culturing cordyceps militaris by living silkworm chrysalises | |
| CN103270887B (en) | Silkworm chrysalis northern Chinese caterpillar Fungus industrial cultivation technique | |
| CN102047811A (en) | High-quality and high-yield culturing method of agrocybe aegerita | |
| CN108782009A (en) | A kind of hickory chick method for increasing | |
| CN106034749B (en) | A kind of ganoderma lucidum and oyster mushroom rotation cropping method | |
| CN105532264A (en) | Out-of-season efficient pleurotus geesteranus cultivation method | |
| CN104521569B (en) | A kind of implantation methods of Auricularia | |
| CN104956912A (en) | Cultivation method for golden mushroom | |
| CN108707527A (en) | High conidia powder cordyceps wine enjoys assembly production method and its culture assembly | |
| CN109220545A (en) | Using pine cedar sawdust as the Sparassis crispa cultivation matrix of raw material and its Sparassis crispa cultural method | |
| CN106508422A (en) | Cultivation method for hypsizygus marmoreus | |
| CN104756754B (en) | A kind of cultural method of Antrodia camphorata fructification | |
| CN102812847A (en) | Cultivating method of pleurotus nebrodensis | |
| CN107586725A (en) | A kind of cordyceps sinensis fluid nutrient medium and the method using medium culture cordyceps sinensis | |
| CN107047068A (en) | Greenhouse mushroom yield-increasing cultivation method | |
| CN107164235A (en) | A kind of breeding method of Cordceps militaris | |
| CN103782912B (en) | Culture medium for red cassia tree tissue culture | |
| CN105724056A (en) | Planting technologies for pleurotus eryngii and pleurotus geesteranus | |
| CN105557303A (en) | Indoor precise cultivation method of pleurotus tuber regium | |
| CN102505009A (en) | Pseudomonas aurantiaca preparation as well as preparation method and application thereof | |
| CN107125025A (en) | A kind of cordyceps militaris cultivation method | |
| CN116396869A (en) | Domesticated strain of Morchella and application thereof | |
| CN110291922A (en) | Breeding method is planted in a kind of high protein tremella room |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170905 |