CN107118998B - Bacterial strains, microbial agents and uses thereof - Google Patents
Bacterial strains, microbial agents and uses thereof Download PDFInfo
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- CN107118998B CN107118998B CN201710564350.4A CN201710564350A CN107118998B CN 107118998 B CN107118998 B CN 107118998B CN 201710564350 A CN201710564350 A CN 201710564350A CN 107118998 B CN107118998 B CN 107118998B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to the technical field of microorganism application, and particularly relates to a bacterial strain, a microbial agent and application thereof. The strain is Vanilla Bacillus B2, is preserved in China general microbiological culture Collection center, and has the preservation number: CGMCC NO. 14090; the preservation time is as follows: year 2017, month 5 and day 4. The microbial inoculum containing the strain is particularly suitable for the rapid degradation of northern corn straws, plays an important role in the field in-situ field returning of crop straws, and simultaneously has the functions of increasing soil humus and improving soil fertility.
Description
Technical Field
The invention relates to the technical field of microorganism application, and particularly relates to a bacterial strain, a microbial agent and application thereof.
Background
The crop straw resources in China are rich, the number is huge, the varieties are various, and the annual crop straw output is 7-8 hundred million tons. Since the straws are not easy to decay in a short period of time and have low utilization efficiency, most straws are abandoned or burnt, and the crop straws become obstacles in production in areas mainly in the planting industry. Therefore, the straw is incinerated throughout vast rural areas, thereby causing environmental pollution and serious waste of organic resources. According to statistics, the crop straws used as returning field and organic fertilizer in China only account for about 20 percent of the total amount, the incineration accounts for 17 percent, and 50 percent of the crop straws are not utilized.
A plurality of provinces in northern areas of China are agricultural provinces, and the demand for straw treatment is more urgent. The northern area has cold weather in autumn and winter, which is not beneficial to the degradation of lignin and cellulose of the corn stalks. Therefore, breaking through the technical bottleneck of large-scale and rapid decomposition and returning of the straws under the condition of adverse climate in northern areas is the problem to be solved urgently in realizing the utilization of the straws in northern areas of China. The method is one of important development trends for solving the problem of straw degradation in northern China.
The straw degradation microorganism is utilized to degrade the straws of corn, wheat and rice and return the straws to the field in situ, so that the method has great application potential. The screening research of the compound bacterial system has also been reported, and the products related to the straw fermentation bacterial agent are very few, and no system is formed, and no stable and efficient straw rapid degradation bacterial flora is produced and used. The rapid straw decomposition technology is not effectively solved, and the efficient utilization of straw resources is limited.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
An aspect of the present invention is to provide a bacterial strain having a cellulose degradation function, which has a high low temperature resistance and a very good cellulose degradation function.
The invention also aims to provide a microbial agent containing the bacterial strains, wherein the mixed microbial agent has obviously stronger cellulose decomposing capacity than any single bacterial strain, and can be well applied to straw degradation.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
according to one aspect of the invention, the bacterial strain is Vanilla Bacillus B2, which is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number: CGMCC NO. 14090; the preservation time is as follows: year 2017, month 5 and day 4.
The Latin school name of the strain is Bacillus vanillicus, the Chinese school name is Vanilla Bacillus, and the strain name is B2.
The strain has a high-efficiency straw degradation function and is high in low-temperature tolerance.
The bacterial colony of the isolated and purified vanilla bacillus is round, milk white and slightly convex in the middle.
The microbial agent containing the vanilla bacillus B2 also belongs to the scope of the present application. For the strain, considering the straw degradation efficiency in application, transportation possibly and other reasons, the strain needs to be expanded and cultured into a microbial agent form to expand the application range of the strain.
Preferably, the microbial agent further comprises one, two or all of the following strains:
bacillus flexus A1 with preservation number of CGMCC NO. 14088;
bacillus thuringiensis A2 with the preservation number of CGMCC NO. 14089; and
bacillus flexus D3 with the preservation number of CGMCC NO. 14091;
the phages are all preserved in the China general microbiological culture Collection center, and the preservation time is as follows: year 2017, month 5 and day 4.
In some embodiments, the microbial agent comprises the bacillus vanilloidis B2 and the bacillus curvatus a 1;
in some embodiments, the microbial agent comprises the bacillus vanilloids B2 and the bacillus thuringiensis a 2;
in some embodiments, the microbial agent comprises the bacillus vanilloidis B2 and the bacillus curvatus D3;
in some embodiments, the microbial agent comprises the bacillus vanilloids B2, the bacillus curvatus a1, and the bacillus thuringiensis a 2;
in some embodiments, the microbial agent comprises the bacillus vanilloidis B2, the bacillus curvatus a1, and the bacillus curvatus D3;
in some embodiments, the microbial agent comprises the bacillus vanilloids B2, the bacillus thuringiensis a2, and the bacillus curvatus D3;
preferably, the microbial agent comprises, in terms of viable count, the mixing ratio of bacillus curvatus A1, bacillus thuringiensis A2, bacillus vanilloidis B2 and bacillus curvatus D3 in the microbial agent is (1-5): (1-5).
More preferably, the agent is a mixture of Bacillus curvatus A1, Bacillus thuringiensis A2, Bacillus vanicus B2 and Bacillus curvatus D3 in equal ratio.
The microbial inoculum is preferably a liquid inoculum, and the liquid inoculum is culturedThe liquid may preferably be a liquid medium such as LB liquid medium, or further supplemented with nutrients such as glucose, thiamine, biotin and nicotinic acid, preferably, in order to achieve good cellulose degradation effect, the number of viable bacteria per strain in the liquid microbial inoculum is not less than 1 × 107CFU/ml, preferably, the number of viable bacteria per strain is 1 × 107-16CFU/ml, more preferably 1 × 108-15CFU/ml, alternatively 1 × 109CFU/ml,1×1010CFU/ml,1×1011CFU/ml,1×1012CFU/ml,1×1013CFU/ml,1×1014CFU/ml, etc.
Preferably, the preparation method of the microbial agent comprises the following steps:
the invention provides a preparation method based on the liquid microbial agent, which comprises the following steps:
inoculating the strain into a liquid culture medium for culturing to obtain a liquid microbial inoculum;
optionally, the liquid inoculum is filtered and/or concentrated.
The whole microbial agent has simple preparation method, can be stored for a short time and is convenient to transport, and can be directly added into soil according to a certain proportion when in use, so that the use is very convenient.
The microbial inoculum is preferably a solid inoculum prepared by adding a solid carrier and/or an auxiliary agent to the strain.
Preferably, the solid carrier includes one or more of grass carbon, bran powder, wheat bran, kaolin, light calcium carbonate, diatomite, white carbon black, talcum powder, fine sand and clay.
Preferably, the microbial agent is one or a mixture of two or more of sucrose, glucose, peptone, soybean meal, sodium dodecylbenzenesulfonate and sodium alkylnaphthalenesulfonate polycondensates.
The invention provides a preparation method based on the solid microbial agent, which comprises the following steps:
and mixing the purified strain with a solid carrier and/or an auxiliary agent to obtain the strain.
The application of the strain or the microbial inoculum in straw digestion.
The microbial inoculum can be used for degradation and in-situ returning of corn, wheat and rice straws.
Compared with the prior art, the invention provides the microbial strain for efficiently degrading the straws and the microbial agent containing the strain, wherein the microbial agent mainly comprises four microbial strains, is particularly suitable for fast degradation of the corn straws in the north, and has an important function in field in-situ returning of the crop straws to the field. Meanwhile, the fertilizer has the functions of increasing soil humus and improving soil fertility.
The Bacillus flexus strain provided by the invention has the strain name of A1 and the preservation number of CGMCC NO. 14088;
the Bacillus thuringiensis provided by the invention has a strain name of A2 and a preservation number of CGMCC NO. 14089;
the bacterial strain name of the vanilla Bacillus (Bacillus vanillica) provided by the invention is B2, and the preservation number is CGMCC NO. 14090;
the strain name of the Bacillus flexus provided by the invention is D3, and the preservation number is CGMCC NO. 14091;
the strains are all preserved in China general microbiological culture Collection center, and the preservation addresses are as follows: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation time is as follows: year 2017, month 5 and day 4. The strains were detected as viable by the depository at 2017, 5 and 4 days.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by manufacturers, and are all conventional products available on the market.
Example 1
This example provides a method for screening individual microorganism strains and a method for combining microbial agents.
1. Experimental Material
1.1 strains and soil
Test strains: the bacterial seed bank stores cellulose-degrading bacteria.
Black soil in northeast: 0-20 cm of surface soil in the field of Jilin princess mountains.
1.2 culture Medium
1.2.1 bacterial culture Medium: 3g of beef extract, 10g of peptone, 5g of NaCl, 16-18 g of agar and 1L of deionized water.
1.2.2 bacterial fermentation Medium (YPD Total nutrient Medium): dissolving yeast extract/yeast powder 10g/L, peptone 20g/L in 1L deionized water, pH 7.0.
1.2.3 cellulose degrading bacteria culture medium: KH (Perkin Elmer)2PO40.5g、MgSO40.25g, peptone 2g, sodium carboxymethylcellulose (CMC)1.88g, Congo red 0.2g, agar 16-18 g, deionized water 1L, and pH 7.0.
1.2.4 basic nutrient solution (hertzson medium): 1.0g of monopotassium phosphate, 0.1g of NaCl and NaNO32.5g, 1L deionized water, and natural pH.
Single strain low-temperature activity screening method
2.1 Strain activation and rejuvenation
The colony in the preservation inclined plane tube is picked up by the inoculating loop and inoculated in a bacterial liquid culture medium (1.2.1) without agar, and is cultured for 72 hours at the temperature of 28 ℃ to prepare a single bacterial strain test bacterial liquid.
2.2 Low temperature Activity screening of strains
Adding 40 g of soil into a 250ml triangular shake flask, spraying water to moisten the soil until the water content is 30-50% of the field water retention rate, sealing a breathable film, carrying out moist heat sterilization for 3-5 times, carrying out plate inspection on the soil without viable bacteria by using a bacterial culture medium (1.2.1), inoculating 2ml of test bacteria liquid, culturing for 3 days at 28 ℃, and then carrying out heat preservation for 24 hours at-20 ℃; the temperature is kept at 20 ℃ for 24h for one cycle. After the freeze thawing cycle is carried out for 9 times, 100ml of sterile water is added into a shake flask, and the shake flask is vibrated repeatedly (the rotating speed is 170r/min) for 30 minutes to prepare the soilAnd (4) diluting the soil suspension step by step, and carrying out viable bacteria counting determination by a flat counting method. The number of cells in the soil after freeze-thaw cycles>1011The inoculated strain was judged to have high activity at low temperature at cfu/g soil.
TABLE 1
Inoculating strains | Count after 9 cycles of freeze-thaw cycle (cfu/g. soil) |
A1 | 1.38×1012 |
A2 | 2.72×1011 |
B1 | 9.50×1012 |
B2 | 1.16×1012 |
D1 | 6.50×1012 |
D2 | 5.60×1016 |
D3 | 1.26×1012 |
2.3 Shake flask method for screening high-efficiency straw degradation bacterial strain
Adding 50mL of basic nutrient solution (1.2.4) and 5g of dried crushed corn straws into a 100mL triangular flask, inoculating high-activity low-temperature strains in the table 1 after sterilization by a wet-heat method, performing shaking culture at 30 ℃ and 170r/min for 10 days, 15 days and 20 days, repeating each treatment for three times, simultaneously performing basic nutrient solution and straw control CK, determining the weight loss rate of the straws cultured for 10 days, 15 days and 20 days, and respectively determining the strains with strong degradation function by increasing the weight loss rate of the straws treated by the inoculated strains by more than 10% compared with the weight loss rate of the straws of the blank control without the inoculated strains.
TABLE 2
TABLE 3
According to tables 2 and 3, the strains with good degradation effect after being inoculated for 10 days comprise A1, A2, B1 and B2, the strains with strong straw degradation function after being inoculated for 15 days comprise B1, B2, D1, D2 and D3, and the high-efficiency straw degradation strains after being inoculated for 20 days comprise A1, A2, B2 and D3.
2.4 Complex strains screening
A combination of A1A 2-type 4B-D strains, i.e., any combination of B1 or B2 strains with D1, D2 or D3 after 2 strains were removed, was set. Mixing the bacterial suspensions of each bacterial strain combination according to the volume ratio of 1: 1, respectively inoculating 6mL of the bacterial suspensions into sterilized vermiculite mixed with 5g of dried straws, keeping the vermiculite in a saturated water absorption state, and culturing for 20 days (25 ℃ day and 20 ℃ night) in an artificial climate chamber; and (4) determining the weight loss rate of the straws at 20d, repeating each time for 3 times, and simultaneously preparing a basic nutrient solution and a straw control CK. The percentage of weight loss ratio and relative blank increase of the inoculated microbial inoculum straw is the decay promoting rate, and the composite bacterial system which is screened is the one with the decay promoting rate more than 10%. As a result of the experiment, 5 groups of complex strains such as A1A2B3D3, A1A2B2D3, A1A2B2D1, A1A2B2D5 and A1A2B2D4 were obtained.
Wherein, A1 corresponds to Bacillus curvatus A1; a2 corresponds to Bacillus thuringiensis A2 deposited in the present invention; b2 corresponds to Vanilla Bacillus deposited herein B2; d3 corresponds to Bacillus curvatus D3;
TABLE 4
Example 2
The embodiment provides a liquid microbial inoculum and a preparation method thereof, and the preparation method specifically comprises the following steps:
preparing single strain bacterial liquid in a bacterial fermentation culture medium (1.2.2), wherein the bran powder is prepared according to a solid-liquid ratio of 1: 2, adsorbing the single bacterium liquid in proportion to prepare single bacterium agents A1, A2, B2 and D3; and (3) mixing the 4 single bacteria agents in equal proportion, air-drying and bagging to complete the preparation of the composite bacteria agent.
The content of each strain in the obtained liquid microbial inoculum is 1 × 1010-11CFU/ml。
Example 3
In this example, the preparation operation was the same as in example 1, and different from example 1, in this example, the single microbial agents a1, B2, D3 were prepared; 3 single bactericides are mixed according to the ratio of viable count of 1:3:5, and are air-dried and bagged to complete the preparation of the composite bactericide.
Example 4
The embodiment provides a solid microbial inoculum and a preparation method thereof, and the preparation method specifically comprises the following steps:
the liquid microbial inoculum prepared in the embodiment 2 is added into talcum powder according to the proportion that 30 g of talcum powder (used as a solid carrier) is added into 1 kg of liquid microbial inoculum, and the mixture is fully stirred, uniformly mixed, filtered, dried and crushed to obtain the solid microbial inoculum.
Example 5
In this example, the procedure was the same as in example 4, except that in this example, the solid carrier used was a mixture of talc, fine sand and clay in equal proportions.
Experimental example field effect verification
The corn is harvested for 10 months and then crushed, 5 kg of the compound microbial inoculum prepared in the embodiment 2 is uniformly spread on the surface of the straw, the straw is buried in the soil by deeply turning over for 30 cm, meanwhile, the straw sample bag is buried, and the decomposition rate of the straw sample (calculated by the weight loss rate) is measured in the spring of the next year.
TABLE 5
TABLE 6
TABLE 7
As shown in the results in tables 6 and 7, the composite microbial inoculum provided by the invention has good corrosion promoting effect on the buried straws in the field in the growth stage of the corn seedling stage and the large flare stage (5-7 months) in spring of princess mountains and double cities.
While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.
Claims (10)
1. The bacterial strain is Vanilla bacillus B2, and is preserved in China general microbiological culture Collection center (CGMCC), wherein the preservation numbers are as follows: CGMCC NO. 14090; the preservation time is as follows: year 2017, month 5 and day 4.
2. A microbial agent comprising the strain of claim 1.
3. The microbial agent according to claim 2, wherein the microbial agent is a liquid microbial agent, and the number of viable bacteria of a strain in the liquid microbial agent is not less than 1 × 107CFU /ml。
4. The microbial agent according to claim 2, wherein the microbial agent is a solid microbial agent prepared by adding a solid carrier and/or an auxiliary agent to the strain.
5. The microbial inoculant according to claim 4, wherein the solid carrier comprises one or more of peat, bran powder, wheat bran, kaolin, precipitated calcium carbonate, diatomaceous earth, white carbon, talc, fine sand and clay.
6. The microbial inoculant according to claim 4, wherein the auxiliaries are selected from one or more of sucrose, glucose, peptone, soybean meal, sodium dodecylbenzene sulfonate, sodium lignosulfonate, sodium alkyl naphthalene sulfonate polycondensates.
7. The method for preparing a microbial agent according to claim 3, comprising the steps of:
and inoculating the strain into a liquid culture medium for culture to obtain a liquid microbial inoculum.
8. The method for preparing a microbial agent according to claim 7, further comprising the steps of: filtering and/or concentrating the liquid microbial inoculum.
9. The process for the preparation of a microbial inoculant according to any one of claims 4 to 6, comprising the steps of:
and mixing the purified strain with a solid carrier and/or an auxiliary agent to obtain the strain.
10. The strain of claim 1 or the microbial agent of any one of claims 2 to 6, for use in straw digestion.
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0866179A (en) * | 1994-08-30 | 1996-03-12 | Fujisawa Pharmaceut Co Ltd | New bacillus sp. bacillus carbophilus |
CN102747001A (en) * | 2011-07-05 | 2012-10-24 | 天津理工大学 | Physical pretreatment fermentation process for corn straws by using flora degradation |
CN103159511A (en) * | 2011-12-16 | 2013-06-19 | 河北农业大学 | Bacillus thuringiensis solid fermentation process and application of same in agriculture |
CN104789503A (en) * | 2015-04-16 | 2015-07-22 | 乐山师范学院 | Straw decomposition microbial agent with insecticidal effect and application thereof |
CN104894007A (en) * | 2015-05-12 | 2015-09-09 | 安徽农业大学 | Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses |
CN106479917A (en) * | 2016-09-30 | 2017-03-08 | 洛阳茂生生物技术有限公司 | A kind of microbial bacterial agent for municipal sludge process |
CN106804628A (en) * | 2017-01-20 | 2017-06-09 | 北京裕丰力多金肥业有限公司 | A kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent and its preparation technology |
-
2017
- 2017-07-12 CN CN201710564350.4A patent/CN107118998B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0866179A (en) * | 1994-08-30 | 1996-03-12 | Fujisawa Pharmaceut Co Ltd | New bacillus sp. bacillus carbophilus |
CN102747001A (en) * | 2011-07-05 | 2012-10-24 | 天津理工大学 | Physical pretreatment fermentation process for corn straws by using flora degradation |
CN103159511A (en) * | 2011-12-16 | 2013-06-19 | 河北农业大学 | Bacillus thuringiensis solid fermentation process and application of same in agriculture |
CN104789503A (en) * | 2015-04-16 | 2015-07-22 | 乐山师范学院 | Straw decomposition microbial agent with insecticidal effect and application thereof |
CN104894007A (en) * | 2015-05-12 | 2015-09-09 | 安徽农业大学 | Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses |
CN106479917A (en) * | 2016-09-30 | 2017-03-08 | 洛阳茂生生物技术有限公司 | A kind of microbial bacterial agent for municipal sludge process |
CN106804628A (en) * | 2017-01-20 | 2017-06-09 | 北京裕丰力多金肥业有限公司 | A kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent and its preparation technology |
Non-Patent Citations (1)
Title |
---|
高效玉米秸秆降解复合菌系的筛选;王珊珊 等;《黑龙江农业科学》;20160627(第4期);第39-41页 * |
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