CN107114609A - A kind of preparation method of complex microorganism additive - Google Patents
A kind of preparation method of complex microorganism additive Download PDFInfo
- Publication number
- CN107114609A CN107114609A CN201710407354.1A CN201710407354A CN107114609A CN 107114609 A CN107114609 A CN 107114609A CN 201710407354 A CN201710407354 A CN 201710407354A CN 107114609 A CN107114609 A CN 107114609A
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- CN
- China
- Prior art keywords
- preparation
- complex microorganism
- microorganism additive
- mixed
- additive
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- 239000000654 additive Substances 0.000 title claims abstract description 20
- 230000000996 additive effect Effects 0.000 title claims abstract description 20
- 244000005700 microbiome Species 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 7
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 7
- 229920001661 Chitosan Polymers 0.000 claims abstract description 7
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 7
- 241000191998 Pediococcus acidilactici Species 0.000 claims abstract description 7
- 230000001580 bacterial effect Effects 0.000 claims abstract description 7
- 239000012530 fluid Substances 0.000 claims abstract description 7
- 239000012456 homogeneous solution Substances 0.000 claims abstract description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 7
- 238000003760 magnetic stirring Methods 0.000 claims abstract description 7
- 239000011259 mixed solution Substances 0.000 claims abstract description 7
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000001694 spray drying Methods 0.000 claims description 12
- 239000006000 Garlic extract Substances 0.000 claims description 3
- 235000011609 Pinus massoniana Nutrition 0.000 claims description 3
- 241000018650 Pinus massoniana Species 0.000 claims description 3
- 235000020706 garlic extract Nutrition 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 229940067866 dandelion extract Drugs 0.000 claims description 2
- 235000020691 dandelion extract Nutrition 0.000 claims description 2
- 239000001845 taraxacum officinale leaf extract Substances 0.000 claims description 2
- 208000011580 syndromic disease Diseases 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 241000251468 Actinopterygii Species 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract 1
- 229940005550 sodium alginate Drugs 0.000 abstract 1
- 239000000661 sodium alginate Substances 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 208000031295 Animal disease Diseases 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/413—Acidilactici
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation method of complex microorganism additive, mainly comprising following step：（1）The bacillus subtilis activated, Pediococcus acidilactici, bacillus licheniformis, Lactobacillus plantarum are inoculated into broth bouillon with 1% concentration respectively and fermented, ferment 36h, single bacterial strain zymotic fluid is obtained, it is then that 4 kinds of single fermentation liquid mixed in equal amounts are uniformly standby；（2）Aseptically above-mentioned mixed fermentation liquid is added in the mixed solution containing 1 2% sodium alginate and chitosan, is sufficiently mixed uniformly with 120r/min on magnetic stirring apparatus, forms homogeneous solution；It is stirred until homogeneous rear mixing for standby use；（3）It is spray-dried, obtains powdery product.The method low manufacture cost of the present invention, effect preferably, overcomes the problem of conventional additive is soluble in water, fish body uptake ratio is low and wastes serious, is especially suitable for promoting the use of.
Description
Technical field
The present invention relates to animal feed, specifically a kind of preparation method of complex microorganism additive.
Background technology
In recent years, with the development of culture fishery, in order to improve aquatic products growth performance, aquaculture cost is reduced, is contained
The feed addictive of the chemical substances such as various hormone quasi-growth promoters, antibiotic is widely used.But such feed
Additive is long-term, largely use, and makes to accumulate substantial amounts of antibiotic, hormonal substance in body, result in organism drug resistance
Produce, water body immunity degradation, or even have a strong impact on the health of people.
The advantage of microorganism and Chinese herbal medicine with its " safety, green, noresidue " has turned into substitute antibiotics, treated and prevented
The most promising substitute of aquatic animal disease.But also there is price height, addition currently used for the microorganism formulation of aquatic products
Larger, cost is high, safety in utilization is low, the low problem of effect of increasing production.
The content of the invention
The purpose of the present invention is to provide a kind of preparation method of complex microorganism additive regarding to the issue above.
Technical scheme：A kind of preparation method of complex microorganism additive, mainly comprising following step：
（1）By the bacillus subtilis activated, Pediococcus acidilactici, bacillus licheniformis, Lactobacillus plantarum respectively with 1% it is dense
Degree, which is inoculated into broth bouillon, to be fermented, and ferment 36h, single bacterial strain zymotic fluid is obtained, then by 4 kinds of single fermentation liquid etc.
Amount is well mixed standby；
（2）Aseptically above-mentioned mixed fermentation liquid is added in the mixed solution containing 1-2% sodium alginates and chitosan,
It is sufficiently mixed uniformly with 120 r/min on magnetic stirring apparatus, forms homogeneous solution；It is stirred until homogeneous rear standby；
（3）It is spray-dried, obtains powdery product.
In the above method, the EAT of spray drying is 120 DEG C.
In the above method, 8 mL of charging rate/min of spray drying.
In order to reach the purpose to prevention from suffering from the diseases, the Pu that the mass concentration that described broth bouillon with the addition of 2-5% is 1%
Public English extract solution.
In order to reach the purpose to prevention from suffering from the diseases, it is 0.5% that described broth bouillon, which with the addition of 1-3% mass concentration,
Needles of pinus massoniana leaf extract.
In order to reach the purpose to prevention from suffering from the diseases, it is 0.5% that described broth bouillon, which with the addition of 1-3% mass concentration,
Garlic extract.
In the above method, the powder can individually be sprinkling upon in fishpond and use, and it sheds concentration for 2-3g/m3。
The beneficial effects of the invention are as follows：The present invention, which makes microorganism and Chinese herbal medicine as raw material, turns into additive, not only
It can add in feed and feed, can also individually be sprinkling upon in fishpond and use, " safely, the additive that the inventive method makes has
Green, noresidue " advantage, can substitute antibiotics, treat and prevent aquatic animal disease, meanwhile, method system of the invention
Make cost low, effect preferably, overcomes the problem of conventional additive is soluble in water, fish body uptake ratio is low and wastes serious,
It is especially suitable for promoting the use of.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment 1
A kind of preparation method of complex microorganism additive, mainly comprising following step：
（1）By the bacillus subtilis activated, Pediococcus acidilactici, bacillus licheniformis, Lactobacillus plantarum respectively with 1% it is dense
Degree, which is inoculated into broth bouillon, to be fermented, and ferment 36h, single bacterial strain zymotic fluid is obtained, then by 4 kinds of single fermentation liquid etc.
Amount is well mixed standby；
（2）Aseptically above-mentioned mixed fermentation liquid is added in the mixed solution containing 1-2% sodium alginates and chitosan,
It is sufficiently mixed uniformly with 120 r/min on magnetic stirring apparatus, forms homogeneous solution；It is stirred until homogeneous rear mixing for standby use；
（3）It is spray-dried, obtains powdery product.
In the above method, the EAT of spray drying is 120 DEG C.
In the above method, 8 mL of charging rate/min of spray drying.
Embodiment 2
A kind of preparation method of complex microorganism additive, mainly comprising following step：
（1）By the bacillus subtilis activated, Pediococcus acidilactici, bacillus licheniformis, Lactobacillus plantarum respectively with 1% it is dense
Degree is inoculated into the addition of to be fermented in broth bouillon of the 2-5% mass concentration for 1% dandelion extract solution, and ferment 36h,
Single bacterial strain zymotic fluid is obtained, it is then that 4 kinds of single fermentation liquid mixed in equal amounts are uniformly standby；
（2）Aseptically above-mentioned mixed fermentation liquid is added in the mixed solution containing 1-2% sodium alginates and chitosan,
It is sufficiently mixed uniformly with 120 r/min on magnetic stirring apparatus, forms homogeneous solution；It is stirred until homogeneous rear mixing for standby use；
（3）It is spray-dried, obtains powdery product.
In the above method, the EAT of spray drying is 120 DEG C.
In the above method, 8 mL of charging rate/min of spray drying.
Embodiment 3
A kind of preparation method of complex microorganism additive, mainly comprising following step：
（1）By the bacillus subtilis activated, Pediococcus acidilactici, bacillus licheniformis, Lactobacillus plantarum respectively with 1% it is dense
Degree is inoculated into the addition of to be fermented in broth bouillon of the 1-3% mass concentration for 0.5% needles of pinus massoniana leaf extract, is sent out
Ferment 36h, obtains single bacterial strain zymotic fluid, then that 4 kinds of single fermentation liquid mixed in equal amounts are uniformly standby；
（2）Aseptically above-mentioned mixed fermentation liquid is added in the mixed solution containing 1-2% sodium alginates and chitosan,
It is sufficiently mixed uniformly with 120 r/min on magnetic stirring apparatus, forms homogeneous solution；It is stirred until homogeneous rear mixing for standby use；
（3）It is spray-dried, obtains powdery product.
In the above method, the EAT of spray drying is 120 DEG C.
In the above method, 8 mL of charging rate/min of spray drying.
Embodiment 4
A kind of preparation method of complex microorganism additive, mainly comprising following step：
（1）By the bacillus subtilis activated, Pediococcus acidilactici, bacillus licheniformis, Lactobacillus plantarum respectively with 1% it is dense
Degree is inoculated into the addition of to be fermented in broth bouillon of the 1-3% mass concentration for 0.5% garlic extract, and ferment 36h,
Single bacterial strain zymotic fluid is obtained, it is then that 4 kinds of single fermentation liquid mixed in equal amounts are uniformly standby；
（2）Aseptically above-mentioned mixed fermentation liquid is added in the mixed solution containing 1-2% sodium alginates and chitosan,
It is sufficiently mixed uniformly with 120 r/min on magnetic stirring apparatus, forms homogeneous solution；It is stirred until homogeneous rear mixing for standby use；
（3）It is spray-dried, obtains powdery product.
In the above method, the EAT of spray drying is 120 DEG C.
In the above method, 8 mL of charging rate/min of spray drying.
Claims (6)
1. a kind of preparation method of complex microorganism additive, it is characterised in that mainly comprising following step：
（1）By the bacillus subtilis activated, Pediococcus acidilactici, bacillus licheniformis, Lactobacillus plantarum respectively with 1% it is dense
Degree, which is inoculated into broth bouillon, to be fermented, and ferment 36h, single bacterial strain zymotic fluid is obtained, then by 4 kinds of single fermentation liquid etc.
Amount is well mixed standby；
（2）Aseptically above-mentioned mixed fermentation liquid is added in the mixed solution containing 1-2% sodium alginates and chitosan,
It is sufficiently mixed uniformly with 120 r/min on magnetic stirring apparatus, forms homogeneous solution；It is stirred until homogeneous rear mixing for standby use；
（3）It is spray-dried, obtains powdery product.
2. the preparation method of complex microorganism additive as claimed in claim 1, it is characterised in that wind-warm syndrome is entered in spray drying
Spend for 120 DEG C.
3. the preparation method of complex microorganism additive as claimed in claim 1, it is characterised in that the charging speed of spray drying
Spend 8 mL/min.
4. the preparation method of complex microorganism additive as claimed in claim 1, it is characterised in that described broth bouillon
The mass concentration that with the addition of 2-5% is 1% dandelion extract solution.
5. the preparation method of complex microorganism additive as claimed in claim 1, it is characterised in that described broth bouillon
The mass concentration that with the addition of 1-3% is 0.5% needles of pinus massoniana leaf extract.
6. the preparation method of complex microorganism additive as claimed in claim 1, it is characterised in that described broth bouillon
The mass concentration that with the addition of 1-3% is 0.5% garlic extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710407354.1A CN107114609A (en) | 2017-06-02 | 2017-06-02 | A kind of preparation method of complex microorganism additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710407354.1A CN107114609A (en) | 2017-06-02 | 2017-06-02 | A kind of preparation method of complex microorganism additive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107114609A true CN107114609A (en) | 2017-09-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710407354.1A Pending CN107114609A (en) | 2017-06-02 | 2017-06-02 | A kind of preparation method of complex microorganism additive |
Country Status (1)
| Country | Link |
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| CN (1) | CN107114609A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821789A (en) * | 2017-11-08 | 2018-03-23 | 广州智龙生物科技有限公司 | A kind of biologic ferment for improving fishes and shrimps intestinal health degree and its preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283940A (en) * | 2013-05-02 | 2013-09-11 | 广西工学院 | Production method of microorganism particle forage |
-
2017
- 2017-06-02 CN CN201710407354.1A patent/CN107114609A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283940A (en) * | 2013-05-02 | 2013-09-11 | 广西工学院 | Production method of microorganism particle forage |
Non-Patent Citations (2)
| Title |
|---|
| 胡锐 等: "中药益生元微胶囊饲料添加剂对斜带石斑鱼生长和免疫性能的影响", 《饲料研究》 * |
| 贺普霄等: "《饲料与绿色食品》", 31 January 2004, 中国轻工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821789A (en) * | 2017-11-08 | 2018-03-23 | 广州智龙生物科技有限公司 | A kind of biologic ferment for improving fishes and shrimps intestinal health degree and its preparation method and application |
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