CN107114562A - A kind of anti-inflammatory has composite plant water extract of antibacterial and anti-oxidation function and its preparation method and application concurrently - Google Patents
A kind of anti-inflammatory has composite plant water extract of antibacterial and anti-oxidation function and its preparation method and application concurrently Download PDFInfo
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Abstract
Composite plant water extract of antibacterial and anti-oxidation function and its preparation method and application is had concurrently the present invention relates to a kind of composite plant water extract and its application in feed and animal health product scope, more particularly to a kind of anti-inflammatory.A kind of anti-inflammatory has antibacterial and anti-oxidation function composite plant water extract concurrently, and the low cost vegetable plant water extract is extracted by the plant material of following components be made by mass percentage：Granatum 4.17%, lemon 54.16%, cassia twig 4.17%, sweet wormwood 16.67%, the bark of official magnolia 20.83%.The present invention gropes to establish a set of Simple process for being applied to extract plant various active composition, it is adaptable to each medium-sized and small enterprises' processing, production.The several plant raw material for being used to constitute composite vegetables extractive in the present invention belongs to low price or medium-priced and at home aboundresources, cost performance height.Compared with original formula, the higher granatum of cost performance is remained, the application dose that significantly reduces the cost of compound and can be substantially reducing on piglet.
Description
Technical field
The present invention relates to a kind of composite plant water extract and its application in feed and animal health product scope, especially relate to
And a kind of anti-inflammatory has composite plant water extract of antibacterial and anti-oxidation function and its preparation method and application concurrently.
Background technology
Feeding antibiotic once served indelible contribution to the development of livestock and poultry breeding industry, but its drug resistance and medicine are residual
The problems such as staying also increasingly highlights.After antibiotic is disabled, the research of Substitutes For Antibiotic also just naturally turns into aquaculture
The problem of primary solution.Plant extracts because rich in the various active composition such as albumen, glucosides and phenolic acid, have concurrently it is antibacterial, anti-oxidant,
The functions such as resisting stress, antiviral and immunological regulation, and the problems such as be not likely to produce bacterial drug resistance and medicament residue, as antibiotic
The study hotspot of substitute.But the extract studied at present is extracted from single plant material mostly, therefore is often existed
The problems such as bioactive ingredients content is relatively low, function is single, effect is not notable.And for composite extract, exist again multiple
The scientific not defect such as strong, adding too much, costly in side.Therefore, if can filter out with a variety of functions and cost performance compared with
High composite vegetables extractive is used to substitute piglet feeding antibiotic, can undoubtedly solve a great problem of livestock breeding industry.Application
The compound plant extract that people's early stage is developed has preferable growth promotion on weanling pig and substitutes feeding antibiotic effect, but because
It is expensive, the problems such as addition is big, limit its application prospect.
The content of the invention
First purpose of the present invention is to provide a kind of composite plant water extract, for substituting feeding antibiotic, final real
The cultivation target of existing " environmentally friendly, nontoxic, safety and green ".Second object of the present invention is to provide above-mentioned composite plant water extraction
The preparation method of thing, this method technique is simple, and cost is low.Third object of the present invention is to provide above-mentioned composite plant water extraction
The application of thing.
In order to realize first above-mentioned purpose, present invention employs following technical scheme：
A kind of anti-inflammatory has antibacterial and anti-oxidation function composite plant water extract concurrently, the low cost vegetable plant water extract by mass percentage by
The plant material of following component, which is extracted, to be made：
Granatum 4.17%
Lemon 54.16%
Cassia twig 4.17%
Sweet wormwood 16.67%
The bark of official magnolia 20.83%.
In order to realize second above-mentioned purpose, present invention employs following technical scheme：
The method of above-mentioned composite plant water extract, this method comprises the following steps：
1）Plant material is ground, mixed, by material-water ratio 1：20 are put into extraction equipment groove, are warming up to 80 DEG C, extract 6h
After go in thickener, proportion is concentrated under the conditions of 0.085MPa, 60 DEG C to 1.25；
2）Enriched product is gone into spray drying device, in 150 DEG C of EAT, air mass flow 500L/H, inlet amount 20%
It is spray-dried under part.
In order to realize the 3rd above-mentioned purpose, present invention employs following technical scheme：
Above-mentioned composite plant water extract is used to prepare the application in the animal feed additive for replacing antibiotic.
Present invention also offers a kind of animal feed additive, the composite plant water extraction described in animal feed additive bag
Thing.
Present invention also offers a kind of animal feed, animal feed includes the composite plant water extract described in power.
In summary, the technical solution adopted by the present invention is as follows：
（1）In order to realize the primary and foremost purpose of the present invention, grope to establish first and a set of be applied to extract plant various active composition
Simple process parameter：Material-water ratio 1：20th, 80 DEG C are extracted after 6h, and concentrated extracting solution proportion is to 1.25 at 60 DEG C, then is sprayed
Mist dries (150 DEG C of EAT, air mass flow 500L/H, inlet amount 20%).
（2）Then using extracorporeal anti-inflammatory, antibacterial and oxidation resistance as evaluation index, screened tentatively from 23 kinds of plant materials
Go out the raw material composition of composite vegetables extractive：Granatum, the bark of official magnolia, cassia twig, sweet wormwood and lemon.Originally formula material is：Granatum,
Folium artemisiae argyi, kadsura longepedunculata and evodia rutaecarpa.
（3）The formula of clear and definite composite vegetables extractive is further tested by Orthogonal Composite（Granatum 1g, cassia twig 1g, it is blue or green
Wormwood artemisia 4g, bark of official magnolia 5g and lemon 13g）And verify its bacteria resistance function（MIC is 125mg/mL）Anti-inflammatory (IC50For 0.138mg/mL) and it is anti-
Oxidability (IC50For 0.40mg/mL).Originally it is formulated（Granatum 1g, folium artemisiae argyi 3g, kadsura longepedunculata 9g and evodia rutaecarpa 9g）And checking
Its bacteria resistance function（MIC is 250mg/mL）With oxidation resistance (IC50For 6.46mg/mL), its anti-inflammatory activity is not detected.
（4）The mg/kgbw of mouse continuous gavage 125,250,500,1000 is given respectively-1The compound extract of dosage 21 days,
The mouse state of mind is good, hair color light, the no abnormality seen phenomena of mortality, after comprehensive analysis, the addition of composite vegetables extractive
Amount should be less than 125 mg/kgbw-1。
（5）0.3mg/ml is added in mouse drinks water（Equivalent to 100 mg/kgbw-1Dosage）21 days, mouse day can be made
Weightening pole significantly improves 13.3%（P=0.008）, feed-weight ratio significantly reduces 10.8%（P<0.05）.Originally formula does not do this content
Research, only finds daily to the mg/kgbw of mouse stomach 1000-1Dosage, continuous gavage 14 days can have mouse daily gain
It is improved trend.
（6）0.3mg/ml is added in mouse drinks water（Equivalent to 100 mg/kgbw-1Dosage）21 days, mouse was carried out
After LPS modelings processing, it is possible to decrease the diarrhea rate of mouse, the height of naps and height of naps/Crypt depth of small intestine are significantly improved
Value（P<0.05）；Significantly reduce small intestinal mucosa proinflammatory factor IL-1 β and IL-6 gene expression（P<0.05）.
（7）Compared with original formula, the consumption of composite vegetables extractive of the invention on mouse is only to be formulated originally
10%, its cost performance is largely improved, there is preferable application prospect in livestock and poultry cultivation.
Advantages of the present invention is as follows：
1st, the present invention gropes to establish a set of Simple process for being applied to extract plant various active composition, it is adaptable to each middle-size and small-size
Enterprise's processing, production.
2nd, the present invention in be used for constitute composite vegetables extractive several plant raw material belong at a low price or medium-priced and
Domestic Resources are abundant, cost performance is high.
3rd, compared with original formula, remain the higher granatum of cost performance, significantly reduce compound cost and can
It is substantially reducing at the application dose on piglet.
Brief description of the drawings
Fig. 1 is the Section for light microscopy figure of lps injection mouse jejunum fine hair after 4 hours.Figure 1A is Normal group（Physiological saline
Inject 4h）, Figure 1B is LPS groups（Lps injection 4h）, Fig. 1 C are anti-inflammatory compound group（Lps injection 4h）.
Fig. 2 is the Section for light microscopy figure of lps injection mouse jejunum fine hair after 24 hours.Fig. 2A is LPS groups（Lps injection
24h）, Fig. 2 B are anti-inflammatory compound group（Lps injection 24h）.
Fig. 3 is the gene expression dose figure of lps injection mouse jejunum mucous membrane proinflammatory factor after 4 hours.
Fig. 4 is the gene expression dose figure of lps injection mouse jejunum mucous membrane proinflammatory factor after 24 hours.
Embodiment
First, the extraction of plant material, screening, compound active composition detection
1st, extract include sweet wormwood, the fruit of glossy privet, lemon, wartwort, cassia twig, granatum, lemon, wilsonii, the root of bidentate achyranthes, gynostemma pentaphylla, ginkgo,
The bark of ash, cordate houttuynia, psoralea corylifolia, the bark of official magnolia, the rhizome of nutgrass flatsedge, Artemisia capillaris, rosemary, Chinese cassia tree, the bighead atractylodes rhizome, barrenwort, radix glycyrrhizae, the leaf of bamboo, mosquito
23 kinds of plant materials such as grass.
2nd, extraction process：Plant material is ground, by material-water ratio 1：20 are put into extraction equipment groove, are warming up to 80
DEG C, gone to after extracting 6h in thickener, proportion is concentrated under the conditions of 0.085MPa, 60 DEG C to 1.25.Enriched product is gone into spray
Mist drying equipment, is spray-dried under the conditions of 150 DEG C of EAT, air mass flow 500L/H, inlet amount 20% to it.
3rd, anti-inflammatory rate is determined：Ultraviolet specrophotometer determines 23 kinds of low cost vegetable plant water extracts to lipoxidase（LOX）Activity
Inhibiting rate is active come the extracorporeal anti-inflammatory for characterizing low cost vegetable plant water extract.Concrete operations are as follows：（1）Test group：Take in 1 μ L 0.5g/mL
Medicinal material water extract is added into the 1.5mL eppendorf pipes for having contained 20 μ L enzyme liquids, after 30 DEG C of water-bath 30min, adds 150 μ L bottoms
Thing, 30 DEG C of 3 min of reaction, adds 500 μ L absolute ethyl alcohol terminating reactions, then adds the mixing of 500 μ L distilled water to be measured；（2）Sun
Property control group：Chinese medicine water extract is substituted with 1 μ L distilled water, remaining step is same（1）；（3）Negative control group：Take 1 μ L Chinese medicines
Water extract, replaces enzyme liquid with 20 μ L distilled water, and remaining step is same（1）；（4）Blank control group：Chinese medicine is substituted with 1 μ L distilled water
Water extract, first adds 500 μ L absolute ethyl alcohol terminating reactions before substrate is added, and remaining step is same（1）.Respectively take 200 μ L prepare liquids
To 96 hole ultraviolet detection plates, the absorbance OD values of 234nm wavelength detecting reaction solutions are chosen on ELIASA.Chinese medicine water extract
Inhibitory activity represents that formula is as follows with anti-inflammatory rate：
ODa, ODb, ODc, ODd represent the OD values of test group, positive controls, negative control group, blank control group respectively.
Inhibitory activity of the low cost vegetable plant water extract of table 1 to lipoxidase（Anti-inflammatory rate）
| Extract species | Cassia twig | The rhizome of nutgrass flatsedge | Artemisia capillaris | The fruit of glossy privet | Barrenwort | Chinese cassia tree |
| Anti-inflammatory rate（%） | 68.06 | 12.0 | 72.01 | 20.0 | 72.3 | 85.66 |
| Extract species | Wartwort | Gynostemma pentaphylla | The root of bidentate achyranthes | The bark of official magnolia | The bighead atractylodes rhizome | Radix glycyrrhizae |
| Anti-inflammatory rate（%） | 89.34 | 32.78 | 6.71 | 91.25 | 5.88 | 1.13 |
| Extract species | Ginkgo leaf | The bark of ash | Palmate meadowsweet | Rosemary | Granatum | Wilsonii |
| Anti-inflammatory rate（%） | 23.5 | 47.4 | 88.92 | 90.84 | 96.06 | 34.29 |
| Extract species | Sweet wormwood | Psoralea corylifolia | Lemon | The leaf of bamboo | Cordate houttuynia | |
| Anti-inflammatory rate（%） | — | — | 91.47 | — | — |
Note：- without antiphlogistic effects
The research (table 1) more than, filtering out the stronger plant material of anti-inflammatory power is：Granatum, lemon magnolia obovata.
4th, bacteriostasis rate is determined：Using Escherichia coli C83912 as strain subject, extract crude drug concentration be 0.5g/mL, by
Volume ratio 1：10 add to each culture medium, inoculation bacterium solution be 1：1000, in 37 DEG C, 210r/min culture 24h on ELISA Plate,
600nm surveys OD values.Bacteriostasis rate calculation formula is：
Bacteriostasis rate of the low cost vegetable plant water extract of table 2 to Escherichia coli
| Extract species | Cassia twig | The rhizome of nutgrass flatsedge | Artemisia capillaris | The fruit of glossy privet | Barrenwort | Chinese cassia tree |
| Bacteriostasis rate | +++ | — | — | — | — | +++ |
| Extract species | Wartwort | Gynostemma pentaphylla | The root of bidentate achyranthes | The bark of official magnolia | The bighead atractylodes rhizome | Radix glycyrrhizae |
| Bacteriostasis rate | — | — | — | + | — | ++ |
| Extract species | Ginkgo leaf | The bark of ash | Palmate meadowsweet | Rosemary | Granatum | Wilsonii |
| Bacteriostasis rate | ++ | — | + | — | +++ | + |
| Extract species | Sweet wormwood | Psoralea corylifolia | Lemon | The leaf of bamboo | Cordate houttuynia | |
| Bacteriostasis rate | +++ | — | — | — | — |
Note：+++ bacteriostasis rate>50%, ++ bacteriostasis rate is 30% ~ 50% ,+bacteriostasis rate<30% ,-without fungistatic effect
Studied more than（Table 2）, filtering out the stronger plant material of bacteriostasis rate is：Granatum, cassia twig and sweet wormwood.
5th, determination oxidative：When Detection and Extraction thing crude drug concentration is respectively 100,50,25,12.5 and 6.25mg/mL,
Using ELISA Plate（100 μ L/ holes）It is 1 in volume ratio：1st, 37 DEG C, under the conditions of 30min to DPPH（0.5mg/mL）Clearance rate,
516nm surveys OD values, by formula：
Calculate free radical scavenging activity and utilize the semi-free base clearance rate of CurveExpert fitting regression equation conversions（IC50）.
Removing IC of the low cost vegetable plant water extract of table 3 to DPPH free radicals50Value（Anti-oxidant IC50）
| Extract species | Cassia twig | The rhizome of nutgrass flatsedge | Artemisia capillaris | The fruit of glossy privet | Barrenwort | Chinese cassia tree |
| Anti-oxidant IC50, mg/ml | 10.41 | 36.08 | 1.04 | 2.55 | 5.34 | 1.5 |
| Extract species | Wartwort | Gynostemma pentaphylla | The root of bidentate achyranthes | The bark of official magnolia | The bighead atractylodes rhizome | Radix glycyrrhizae |
| Anti-oxidant IC50, mg/ml | 13.08 | 1.1 | 199.27 | 1.54 | 10.91 | 78.45 |
| Extract species | Ginkgo leaf | The bark of ash | Palmate meadowsweet | Rosemary | Granatum | Wilsonii |
| Anti-oxidant IC50, mg/ml | 2.87 | 0.71 | 8.52 | 0.31 | 0.08 | 50.62 |
| Extract species | Sweet wormwood | Psoralea corylifolia | Lemon | The leaf of bamboo | Cordate houttuynia | |
| Anti-oxidant IC50, mg/ml | 2.71 | 149.17 | 20.56 | 25.34 | 5.19 |
Studied more than（Table 3）, filtering out the stronger plant material of oxidation resistance is：Granatum.
5th, quadratic-orthogonal experiment：Using L16(54) experimental design（Table 4）, prepare respectively containing lemon, the bark of official magnolia, sweet wormwood, cassia twig
With 16 kinds of composite vegetables extractives of granatum different proportion and detect its In Vitro Bacteriostasis rate and oxidation resistance, tied according to experiment
Fruit show that best complex extracts composition formula granatum 1g, cassia twig 1g, sweet wormwood 4g, bark of official magnolia 5g, lemon 13g）And verify its antibacterial work(
Energy（MIC is 125mg/mL）Anti-inflammatory (IC50For 0.138mg/mL) and oxidation resistance (IC50For 0.40mg/mL).
The Orthogonal Composite Design form of 4 five kinds of plant materials of table
In Vitro Bacteriostasis rate, anti-inflammatory rate and the anti-oxidant IC of each combination low cost vegetable plant water extract of the orthogonal design of table 550Value
| Combination | Bacteriostasis rate（%） | Anti-inflammatory rate（%） | Anti-oxidant IC50（mg/mL） |
| 1 | 34.53 | 86.93 | 0.82 |
| 2 | 33.73 | 78.53 | 0.82 |
| 3 | -4.99 | 69.55 | 0.67 |
| 4 | 36.53 | 75.03 | 0.41 |
| 5 | 26.95 | 57.12 | 0.29 |
| 6 | 31.74 | 73.07 | 0.27 |
| 7 | 17.76 | 79.87 | 0.48 |
| 8 | 38.52 | 94.31 | 0.69 |
| 9 | 59.08 | 72.21 | 0.72 |
| 10 | 79.44 | 91.81 | 2.01 |
| 11 | -1.20 | 91.67 | 0.33 |
| 12 | 79.64 | 92.81 | 0.26 |
| 13 | 60.50 | 73.51 | 0.29 |
| 14 | 28.54 | 79.71 | 0.25 |
| 15 | 81.04 | 93.47 | 1.09 |
| 16 | 71.06 | 103.91 | 0.72 |
According to orthogonal design and the antibacterial of each combination, anti-inflammatory and anti-oxidant result（Table 5）, using the clear and definite anti-inflammatory of range analysis method
The influence factor of effect is followed successively by：Lemon>Granatum>The bark of official magnolia>Sweet wormwood>Cassia twig, extracorporeal anti-inflammatory best results and have concurrently it is antibacterial and
The combined horizontal of anti-oxidation function is：Granatum is 1, and cassia twig is 1, and sweet wormwood is 1, and the bark of official magnolia is 4, and lemon is 4, and each level is corresponding
Material quality be respectively 1g, 1g, 4g, 5g and 13g, the compound is labeled as anti-inflammatory compound.Clearly resisted using range analysis method
The influence factor of oxidation effectiveness is followed successively by：Granatum>Cassia twig>The bark of official magnolia>Lemon>Sweet wormwood, Antioxidtive Activities in Vitro is optimal and has concurrently
It is antibacterial to be with anti-inflammatory properties combined horizontals：Granatum is 4, and cassia twig is 1, and sweet wormwood is 1, and the bark of official magnolia is 2, and lemon is 4, each level phase
Corresponding material quality is respectively 7g, 1g, 4g, 3g and 13g, and the compound is labeled as anti-oxidant compound.
6. the preparation of composite vegetables extractive, antibacterial, anti-inflammatory and Antioxidative Activity Determination
The formula of composite vegetables extractive is as follows：
Granatum 4.17%
Lemon 54.16%
Cassia twig 4.17%
Sweet wormwood 16.67%
The bark of official magnolia 20.83%.
Preparation method：1）Plant material is ground, by material-water ratio 1：20 are put into extraction equipment groove, are warming up to 80
DEG C, gone to after extracting 6h in thickener, proportion is concentrated under the conditions of 0.085MPa, 60 DEG C to 1.25；
2）Enriched product is gone into spray drying device, in 150 DEG C of EAT, air mass flow 500L/H, inlet amount 20%
It is spray-dried under part.
（1）Anti-inflammatory IC50The detection of value：By water extract proportional diluted, the anti-inflammatory rate of each concentration gradient is determined, method is same
On.Further according to anti-inflammatory rate and water extract concentration, regression equation is fitted using CurveExpert softwares, when calculating anti-inflammatory rate is 50%
Water extract concentration, i.e. anti-inflammatory IC50。
（2）The detection of antibacterial MIC value：Using constant broth dilution method.Compound water extract is concentrated into crude drug concentration 1g/
ML, with MH fluid nutrient mediums with 1:The Maxwell of 1000 dilution proportion 0.5 is inoculated with bacterium solution.Sterilizing test tubes are taken to add the sterile broth cultivations of 1mL
Base is supported, water extract concentration of the doubling dilution into each test tube is respectively 500,250,125,62.5 and 31.25 mg/mL；And simultaneously
Prepare positive control and negative control.All test tubes are placed in 37 DEG C of constant incubators and cultivate 24h.After culture terminates, observation
Bacterial growth situation, the sterile test tube of least concentration is the minimal inhibitory concentration of the water extract（MIC）.
（3）Anti-oxidant IC50The detection of value：Method is ibid.
Antibacterial MIC, the anti-inflammatory IC of the anti-inflammatory compound of table 6 and anti-oxidant compound50And anti-oxidant IC50As a result
From table 6, the antibacterial MIC value of anti-inflammatory prescription is 125mg/mL, anti-inflammatory IC50It is worth for 0.14, anti-oxidant IC50It is worth and is
0.40。
7. application effect of the composite vegetables extractive of various dose on mouse
54 Balb/c female mices are chosen, 3 processing are randomly divided into by body weight：Control group（Gavage physiological saline）, anti-inflammatory is multiple
Side's group and anti-oxidant compound group, two compounds are all provided with 125,250,500,1000 mg/kgbw-14 levels（The corresponding water of gavage
Extract）, each 6 mouse of level, 21 days experimental periods.During whole feeding experiment, all experiment mice state of mind are good, hair
Coloured light is bright, the no abnormality seen phenomena of mortality；From table 7-12,2 compound high dose additions can be dirty to mouse daily gain and part
Device index causes to significantly affect（P<0.05）, the part organ index of particularly anti-oxidant compound mouse is even in 250mg/kg
bw-1During dosage still with control group significant difference（P<0.05）.Therefore the growth-promoting effect on mouse only has been carried out to anti-inflammatory compound
Research.
The daily gain and feed intake of the different disposal group mouse of table 7
Note：* represent there is significant difference P compared with control group<0.05
Organ index --- the liver of the different disposal group mouse of table 8
Organ index --- the kidney of the different disposal group mouse of table 9
Organ index --- the thymus gland of the different disposal group mouse of table 10
Organ index --- the spleen of the different disposal group mouse of table 11
Organ index --- the heart of the different disposal group mouse of table 12
8. research of the anti-inflammatory compound to mouse growth-promoting effect
60 15g or so Balb/c female mices are chosen, are divided into 2 groups：Control group（Normal water）, anti-inflammatory compound group（Drinking-water
In add 0.3mg/ml）, every group sets 3 repetitions, 21 days experimental periods.Result of the test shows（Table 13）：Compared with control group, anti-inflammatory is multiple
Mouse daily gain pole can be made to significantly improve 13.3%（P=0.008）, feed-weight ratio significantly reduces 10.8%（P<0.05）.
Influence of the anti-inflammatory compound of table 13 to mouse growth performance
| Control | Anti-inflammatory compound group | |
| Daily ingestion amount g | 3.11±0.09 | 3.25±0.08 |
| Daily gain g | 0.15±0.01a | 0.17±0.01b |
| Feed-weight ratio | 21.21±1.63a | 18.91±1.09b |
9. protective effect of the anti-inflammatory compound to mouse intestinal health
45 Balb/c female mices are chosen, are divided into 3 processing：Control group（Normal water）, LPS groups（Normal water）, LPS+
Compound group（0.3mg/ml anti-inflammatory compounds are added in drinking-water）, feeding experiment was to the 21st day, control group injecting normal saline, and other 2
Group injection LPS, respectively at 4h after lps injection（Every group 8）And 24h（Every group 7）Butcher, sample.From Fig. 1 and Fig. 2,
4h result of the tests show after lps injection（Table 14-16）：LPS group mouse are all suffered from diarrhoea, and diarrhea rate is 100%, with compareing
Group is compared, and Jejunum Crypt depth CD is significantly raised（P<0.05）, VH/CD significantly reduces（P<0.05）；LPS+ compound group mouse
Diarrhea rate is 75%, and compared with LPS groups, VH/CD is significantly raised（P<0.05）；LPS+ compound group IL-1 β and IL-6 gene table
40.1% and 60.8% are reduced respectively up to level（P<0.01）.24h result of the tests show after Fig. 3 and Fig. 4, lps injection,
LPS group diarrhea of mouse rate is 71.43%；Compared with LPS groups, LPS+ compound group jejunums VH is significantly raised（P<0.05）、IL-1β
52.27% and 59.46% are reduced respectively with IL-6 gene expression dose（P<0.01）.
4h and 24h different disposals group diarrhea of mouse situation after the lps injection of table 14
Different disposal group mouse jejunum height of naps after the lps injection 4h of table 15, Crypt depth and height of naps/Crypt depth
Value
| Control group | LPS groups | LPS+ compound groups | |
| Height of naps（VH） | 474.23±41.25 | 431.55±42.14 | 457.79±44.13 |
| Crypt depth（CD） | 155.90±21.92a | 225.54±27.05b | 201.04±30.45b |
| Height of naps/Crypt depth（VH/CD） | 3.23±0.28c | 1.95±0.11a | 2.35±0.25b |
Different disposal group mouse jejunum height of naps, Crypt depth and height of naps/Crypt depth after the lps injection 24h of table 16
| LPS groups | LPS+ anti-inflammatory compound groups | |
| Height of naps（VH） | 331.00±60.26a | 462.49±38.93b |
| Crypt depth（CD） | 168.15±15.74a | 219.87±31.75a |
| Height of naps/Crypt depth（VH/CD） | 2.01±0.34a | 2.20±0.30ab |
Claims (5)
1. a kind of anti-inflammatory has antibacterial and anti-oxidation function composite plant water extract concurrently, it is characterised in that the low cost vegetable plant water extract presses matter
Measure percentages and extract obtained by the plant material of following components：
Granatum 4.17%
Lemon 54.16%
Cassia twig 4.17%
Sweet wormwood 16.67%
The bark of official magnolia 20.83%.
2. a kind of method of the composite plant water extract prepared described in claim 1, it is characterised in that this method includes following
Step：
1）Plant material is ground, by material-water ratio 1：20 are put into extraction equipment groove, are warming up to 80 DEG C, are gone to after extracting 6h
In thickener, proportion is concentrated under the conditions of 0.085MPa, 60 DEG C to 1.25；
2）Enriched product is gone into spray drying device, in 150 DEG C of EAT, air mass flow 500L/H, inlet amount 20%
It is spray-dried under part.
3. the composite plant water extract described in claim 1 is used to prepare answering in the animal feed additive for replacing antibiotic
With.
4. a kind of animal feed additive, this feature is that animal feed additive includes the composite plant described in claim 1
Water extract.
5. a kind of animal feed, this feature is that animal feed includes the composite plant water extract described in claim 1 or 2.
Priority Applications (1)
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| US20130078322A1 (en) * | 2007-12-28 | 2013-03-28 | Liveleaf, Inc. | Therapeutic composition produced using camellia sinensis leaves and hydrogen peroxide |
| CN104274543A (en) * | 2014-09-29 | 2015-01-14 | 浙江海洋学院 | Medicine for treating sessilinasis of rainbow trout |
| CN105267396A (en) * | 2015-11-25 | 2016-01-27 | 严瑾 | Antibacterial anti-inflammatory composition as well as preparation method and pharmaceutical preparation thereof |
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| US20130078322A1 (en) * | 2007-12-28 | 2013-03-28 | Liveleaf, Inc. | Therapeutic composition produced using camellia sinensis leaves and hydrogen peroxide |
| CN104274543A (en) * | 2014-09-29 | 2015-01-14 | 浙江海洋学院 | Medicine for treating sessilinasis of rainbow trout |
| CN105267396A (en) * | 2015-11-25 | 2016-01-27 | 严瑾 | Antibacterial anti-inflammatory composition as well as preparation method and pharmaceutical preparation thereof |
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| 冯彬彬主编: "《中药药理与应用》", 30 November 2015, 中国中医药出版社 * |
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