CN107114371A - Zymosan is used for the new application of Antiphytoviral - Google Patents
Zymosan is used for the new application of Antiphytoviral Download PDFInfo
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- CN107114371A CN107114371A CN201710421948.8A CN201710421948A CN107114371A CN 107114371 A CN107114371 A CN 107114371A CN 201710421948 A CN201710421948 A CN 201710421948A CN 107114371 A CN107114371 A CN 107114371A
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- zymosan
- disease
- virus
- antiphytoviral
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to the new application that zymosan is used for Antiphytoviral, the medicinal usage with antivirus action is prepared more particularly, to zymosan.Zymosan involved in the present invention has prevention effect to various plants virosis, such as tobacco mosaic virus disease, yellow stunt of wheat viral disease or oryza virus 3 disease.It can use method known in the art that the present invention zymosan is made into pharmaceutical composition, and acceptable formulation, such as aqua, soluble liquid, missible oil, microemulsion, suspending agent, aqueous emulsion, wettable powder or water dispersible granules pharmaceutical composition in any Pesticide Science.In addition, zymosan is acted on people and animals and ecological environment nonhazardous, it is development green, the ideal biological agricultural chemicals of organic agriculture.
Description
Technical field
The present invention relates to technical field of pesticide, particularly Antiphytoviral pesticide field, yeast is further related to many
Sugar acts on antiviral new application.
Background technology
The viroses of plant are the important plant diseases of a class, the whole world it is annual because the viroses of plant endanger and caused by economical damage
Up to 60,000,000,000 dollars are lost, wherein only just up to 20,000,000,000 dollars of the loss of cereal crops one.Plant virus does not have complete thin
Born of the same parents' structure, it is absolute parasitic to plant, and along with plant lacks complete immune system in itself, plant is once invaded by virus
After dye, just band is malicious throughout one's life, continues to suffer from harm, this brings great difficulty to the preventing and treating of the viroses of plant.At present, agricultural is raw
Do not have specific Antiphytoviral medicament in production also.It is serious in face of viroses of plant harm, the office of Antiphytoviral medicament shortage
Face, develops new anti-phytoviral activity composition and studies its antiviral-mechanism and have great importance.In recent years, it is high living
Property, good hypotoxicity, Environmental compatibility, Small side effects biological source polysaccharide, glycopeptide, the research of protein isoreactivity composition are received
The favor of numerous researchers.More there are numerous studies to show, many biological source polysaccharide, glycopeptide and oligosaccharides etc. are to plant defense
There is preferable immunoregulation to act on for the invasion of pathogen.These exploitations for being found to be Antiphytoviral medicament provide new think of
Road.
This laboratory early stage carried out the screening of biological source anti-phytoviral activity material, the separation of active component identification and
Its antiviral-mechanism is studied, and zymosan is obtained in microbial source material and is had to tobacco mosaic virus (TMV) and luteovirus etc.
There is higher inhibitory activity.Zymosan molecular structure stabilized, can extract largely to obtain, it ferments by the fermentation of saccharomycete
Procurement cost is low, extraction process is simple and is not limited by external environmental condition.Importantly, the zymosan is microorganism
Source natural products, with being had no side effect to person poultry safety, to plant, noresidue and is not likely to produce drug-fast feature, is agricultural
Hypotoxicity, the antiviral substance of high activity being badly in need of in production, the potentiality with great commercial application.
On the bioactivity of zymosan, forefathers think the polysaccharide have clearing heat and detoxicating, antitumor, strengthen immunity and
Hypotensive etc. is acted on, and in terms of the activity and its mechanism of action of Antiphytoviral, related research report is at home and abroad had no always
Road.This laboratory is found that it has higher anti-phytoviral activity and further studies its antiviral-mechanism, finds yeast
Polysaccharide processing is remarkably improved the antiviral activity of plant.
The content of the invention
The invention provides the new application that a kind of zymosan is used for Antiphytoviral.
Further, since the zymosan of the present invention has good inhibitory action to various plants virus, the present invention is also carried
The medicinal usage with antivirus action is prepared for a kind of zymosan.
Above-mentioned zymosan is used to prevent and treat tobacco mosaic virus disease (Tobacco mosaic virus, TMV), wheat yellow dwarf
Virosis (Barley yellow dwarf virus, BYDV) or oryza virus 3 disease (Rice stipe virus,
RSV), compared to existing antivirotic, the antivirotic such as with Ningnanmycin for main component, with more preferable effect,
When its concentration is 500 μ g/ml, the prevention effect to tobacco mosaic virus (TMV) is at least 75%.
The pharmaceutical composition containing zymosan can be made using method known in the art in above-mentioned zymosan, with
And acceptable formulation in any Pesticide Science, such as aqua, soluble liquid, missible oil, microemulsion, suspending agent, aqueous emulsion, can
WP or water dispersible granules pharmaceutical composition etc..
Above-mentioned zymosan has significant growth-promoting functions to tobacco, and has to person poultry safety, plant is made without pair
With, noresidue and drug-fast feature is not likely to produce, is hypotoxicity, the antiviral substance of high activity being badly in need of in agricultural production,
Potentiality with very big commercial application.
Brief description of the drawings
Fig. 1：Half leaf method is determined after therapeutic effect of the zymosan to TMV, tobacco leaf inoculation TMV viruses, left side blade
Handled with zymosan, right side blade water process is used as control.A：Zymosan concentration is 500 μ g mL-1；B：Zymosan
Concentration is 1000 μ g mL-1；
Fig. 2：Dot-ELISA experiments determine inhibitory activity of the zymosan to TMV viruses, and the spot colors depth represents virus
Content height, 1：Positive control, 2：Negative control, 3：500μg·mL-1Zymosan processing, 4：1000μg·mL-1Yeast is more
Sugar processing.
Embodiment
In order to understand the present invention, the present invention is further illustrated with embodiment below, but following embodiments are not intended to limit this hair
It is bright.
Embodiment 1：Indoor therapeutic effect of the zymosan to TMV viruses.
(1) experiment condition：Crop choice indoor pot tobacco, kind is Nicotiana glutinosa；Target is TMV.
(2) experimental design and arrangement：Test medicine is zymosan, sets up 8% Ningnanmycin aqua comparison medicament and clear
Water is compareed.3 processing, are repeated 3 times.
(3) application method：Nicotiana glutinosa length is to the 5-6 leaf phases, for testing.Using half leaf withered spot method, whole blade is first inoculated with
TMV viruses, after being inoculated with 2 hours, half leaf of a blade left side smears zymosan or comparison medicament, and right half leaf smears clear water and is used as control.
(4) investigation method：5 days after daubing medicament, the withered spot quantity that statistics left and right half vane is produced, according to equation below meter
Calculate prevention effect：
Prevention effect (%)=(right half leaf withered spot number-left half leaf withered spot number)/left half leaf withered spot number × 100
(5) experimental result：Zymosan is shown in Table 1 to TMV therapeutic effect, by the visible zymosan of table 1 concentration be 500
μg mL-1When be 75.1% to TMV indoor therapeutic effect.
Indoor control effect of the zymosan of table 1 to tobacco mosaic virus disease
Embodiment 2：Control in field effect of the zymosan to tobacco mosaic virus disease.
(1) experiment condition：Crop choice crop field tobacco, kind is K326；Target is TMV.
(2) experimental design and arrangement：Test medicine is zymosan, sets up 8% Ningnanmycin aqua comparison medicament and clear
Water is compareed.3 processing, are repeated 3 times, 9 cells totally.Experimental plot is arranged by random district's groups.
(3) application method：Carry out spray pesticide within 20 days after tobacco transplant, sprayed every second after 7 days.
(4) investigation method：Efficacy survey is carried out after second of dispenser after 14 days, according to following standard recording incidence：
0 grade：Complete stool is disease-free；1 grade：Young leaves shows slight floral leaf；2 grades：/ to two/3rd blade shows floral leaf；
3 grades：2/to three/2nd blades show floral leaf；4 grades：Complete stool blade floral leaf.
Disease index=∑ (diseased plant numbers at different levels × relative value of series)/(investigation total strain number × highest ranked value) × 100%
Prevention effect (%)=(control disease index-processing disease index)/control disease index × 100
(5) experimental result：Zymosan is shown in Table 2 to the prevention effect of tobacco mosaic virus disease, by the visible zymosan of table 2
It is 500 μ g mL in concentration-1When be 75.0% to the prevention effect of tobacco mosaic virus disease.
Control in field effect of the zymosan of table 2 to tobacco mosaic virus disease
Embodiment 3：Control in field effect of the zymosan to yellow stunt of wheat viral disease.
(1) experiment condition：Crop choice crop field wheat, kind is No.6 Xiaoyan；Target is BYDV.
(2) experimental design and arrangement：Test medicine is zymosan, sets up 8% Ningnanmycin aqua comparison medicament and clear
Water is compareed.3 processing, are repeated 3 times, 9 cells totally.Experimental plot is arranged by random district's groups.
(3) application method：Carry out spray pesticide within 20 days after wheat is turned green, sprayed every second after 7 days.
(4) investigation method：Efficacy survey is carried out after second of dispenser after 14 days, investigate 10 plants of connected wheats, often at every
Cell investigates 250 plants, according to following standard recording incidence：
0 grade：Complete stool is disease-free；1 grade：Part blade tip yellow；2 grades：The lower 1 leaf yellow of boot leaf；3 grades：The lower 2 leaf Huangs of boot leaf
Change；4 grades：Boot leaf yellow 1/4, the lower 1 leaf yellow of boot leaf；5 grades：Boot leaf yellow 1/4, the lower 2 leaf yellows of boot leaf；6 grades：Boot leaf is yellow
Change；7 grades：Boot leaf yellow, the lower 1 leaf yellow of boot leaf；8 grades：Boot leaf yellow, the lower 2 leaf yellows of boot leaf；9 grades：Plant is downgraded, but
Can heading；10 grades：Plant is downgraded notable, it is impossible to ear.
Disease index=∑ (diseased plant numbers at different levels × relative value of series)/(investigation total strain number × highest ranked value) × 100%
Prevention effect (%)=(control disease index-processing disease index)/control disease index × 100
(5) experimental result：Zymosan is shown in Table 3 to the prevention effect of yellow stunt of wheat viral disease, by the visible zymosan of table 3
It is 500 μ g mL in concentration-1When be 77.7% to the prevention effect of yellow stunt of wheat viral disease.
Control in field effect of the zymosan of table 3 to yellow stunt of wheat viral disease
Embodiment 4：Control in field effect of the zymosan to oryza virus 3 disease.
(1) experiment condition：Crop choice field rice, kind is two line system；Target is RSV.
(2) experimental design and arrangement：Test medicine is zymosan, sets up 8% Ningnanmycin aqua comparison medicament and clear
Water is compareed.3 processing, are repeated 3 times, 9 cells totally.Experimental plot is arranged by random district's groups.
(3) application method：Carry out spray pesticide within 20 days after rice transplanting, sprayed every second after 7 days.
(4) investigation method：Efficacy survey is carried out after second of dispenser after 14 days, investigate 10 plants of connected paddy rice, often at every
Cell investigates 250 plants, according to following standard recording incidence：
0 grade：Complete stool is disease-free；1 grade：There is a small amount of chlorisis macula lutea along vein in lobus cardiacus base portion, not curled；3 grades：Newborn Leaves occur
The yellowish green alternate striped parallel with vein, slight curling；5 grades：There is yellowish green alternate striped largely parallel with vein in Newborn Leaves,
It is leaf rolling, thin and delicate；7 grades：Plant is downgraded, and blade yellow-white striped occurs and rolled, and Newborn Leaves distortion is sagging, it is impossible to just normally opened
；9 grades：Plant is seriously downgraded, chlorosis or death.
Disease index=∑ (diseased plant numbers at different levels × relative value of series)/(investigation total strain number × highest ranked value) × 100%
Prevention effect (%)=(control disease index-processing disease index)/control disease index × 100
(5) experimental result：Zymosan is shown in Table 4 to the prevention effect of oryza virus 3 disease, by the visible yeast of table 4
Polysaccharide is 500 μ g mL in concentration-1When to oryza virus 3 disease prevention effect be 81.6%.
Control in field effect of the zymosan of table 4 to yellow stunt of wheat viral disease
Claims (8)
1. zymosan is used for the purposes of Antiphytoviral.
2. zymosan prepares the medicinal usage acted on Antiphytoviral.
3. purposes according to claim 1 or 2, zymosan is used to prevent and treat crop disease viral disease.
4. purposes according to claim 1 or 2, wherein in antiviral purposes, the consumption of zymosan is >=100.0
μg/ml。
5. purposes according to claim 1 or 2, wherein in antiviral purposes, the consumption of zymosan is 100.0-
2000.0μg/ml。
6. purposes according to claim 1 or 2, wherein zymosan are made or manual from nature or biotechnology
Learn synthesis.
7. zymosan according to claim 6 is made from biotechnology, it is characterised in that essentially from saccharomycete warp
The method such as physical wall breaking or enzymolysis or acidolysis is made.
8. crop disease viral disease according to claim 3 be tobacco mosaic virus disease (Tobacco mosaic virus,
TMV), yellow stunt of wheat viral disease (Barley yellow dwarf virus, BYDV) or oryza virus 3 disease (Rice
Stipe virus, RSV).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710421948.8A CN107114371A (en) | 2017-05-26 | 2017-05-26 | Zymosan is used for the new application of Antiphytoviral |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710421948.8A CN107114371A (en) | 2017-05-26 | 2017-05-26 | Zymosan is used for the new application of Antiphytoviral |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107114371A true CN107114371A (en) | 2017-09-01 |
Family
ID=59730141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710421948.8A Pending CN107114371A (en) | 2017-05-26 | 2017-05-26 | Zymosan is used for the new application of Antiphytoviral |
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| Country | Link |
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| CN (1) | CN107114371A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832300A (en) * | 2019-03-08 | 2019-06-04 | 成都新朝阳作物科学有限公司 | The composition pesticide and its preparation method and application for preventing and treating the viroses of plant |
-
2017
- 2017-05-26 CN CN201710421948.8A patent/CN107114371A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832300A (en) * | 2019-03-08 | 2019-06-04 | 成都新朝阳作物科学有限公司 | The composition pesticide and its preparation method and application for preventing and treating the viroses of plant |
| CN109832300B (en) * | 2019-03-08 | 2021-10-26 | 成都新朝阳作物科学股份有限公司 | Pesticide composition for preventing and treating plant virus diseases and preparation method and application thereof |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170901 |