CN105900983A - Application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance - Google Patents
Application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance Download PDFInfo
- Publication number
- CN105900983A CN105900983A CN201610277808.3A CN201610277808A CN105900983A CN 105900983 A CN105900983 A CN 105900983A CN 201610277808 A CN201610277808 A CN 201610277808A CN 105900983 A CN105900983 A CN 105900983A
- Authority
- CN
- China
- Prior art keywords
- plant
- leaf curl
- phytosulfokine
- preparation
- yellow leaf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses an application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance and an application of the phytosulfokine-alpha in preparing preparations capable of improving plant yellow leaf curl virus resistance. According to the preparations prepared by the active ingredient, namely the phytosulfokine-alpha, signal paths of auxin in plants are induced to improve the resistance to the yellow leaf curl virus remarkably, so that economic loss caused by the yellow leaf curl virus to plants is decreased. The way of applying the preparations to prevent the plant yellow leaf curl virus is simple and easy and low in cost, capable of delaying and suppressing growth of the plant yellow leaf curl virus on leaves and spreading of diseases remarkably and improving the plant yellow leaf curl virus resistance greatly.
Description
Technical field
The present invention relates to chemical protection of plant technical field, particularly relate to phytosulfokine-α-α and improving aetiolation Qu Ye
Application in virosis resistance.
Background technology
Fructus Lycopersici esculenti is occupied first place in the world in cultivated area and the equal position of total output of China, and demand and cultivated area are all increasing year by year
Add.The production safety of Fructus Lycopersici esculenti in virosis serious threat, wherein by tomato yellow leaf curl virus (Tomato yellow leaf
Curl virus, TYLCV) virosis late 1930s of causing finds first in Israel, passes the nineties in 20th century
Enter China, become the destructive disease of serious harm tomato production.
The primary vehicle of tomato yellow leaf curl China virus is Bemisia tabaci, can infect the crops such as Solanaceae, pulse family and Cruciferae.Should
Sick symptom on Fructus Lycopersici esculenti mainly shows themselves in that primary growth is slow or stagnates, delay of blooming, flower and solid minimizing.Plant is bright
Aobvious dwarfing, vein purpling, deformable blade is shrivelled, and yellowish green uneven speckle occurs in young leaves, has rough shrinkage or deformation, seriously
Time blade atrophy, Post flowering not result or many malformed fruits, yield and quality reduce and even have no harvest, and cause sternly to grower
The economic loss of weight.
Deriving from that the tomato yellow leaf curl China virus resistant gene of wild species is at present the most after positioning 5, China utilizes resistance base
Because having given birth to some resistant varieties.But disease-resistant varieties generally comprises only 1 or 2 resistant genes mostly, in the face of TYLCV's
Easily variability, the usual resistance of such kind is unstable.In addition, it is susceptible for producing the upper tomato variety majority promoted at present
Kind.In production, it is preventing and treating tomato yellow leaf curl virus disease and the main path passing poison medium that chemical pesticide is used.
Wherein, Junkeduke, ZhiBingLing(SIC) or Moroxydine ethyl ketone etc. are used to prevent and treat virosis, but the actual preventive effect in field
Difference；Imidacloprid, fourth sulfur imidacloprid, buprofezin, ethiprole etc. are used to kill Bemisia tabaci, but very big at Bemisia tabaci population number
In the case of, these insecticides are the best to the effect that preventing and treating tomato yellow leaf curl virus is sick, and have asking of pesticide residues
Topic.Therefore, finding the disease control means of a kind of effective, harmless is crops tomato yellow leaf curl China viral disease study on prevention and sending out from now on
The direction of exhibition.
On Induced Disease Resistance In Plants is the defence system utilizing plant itself, excites plant entirety resistance by means of inducible factor
Level, makes original susceptible reaction produces local or the resistance of system, improves the ability of opposing disease, exempt from also known as acquired
Epidemic disease, is a kind of new approaches of control of plant disease.
In general, plant produces systemic disease resistance by inducer, is transferred to whole plant by endogenous signal subsequently,
Produce a series of biochemical reactions, the disease-resistant gene in activated plant body, produce some materials with antiviral activity.
On Induced Disease Resistance In Plants has the advantages such as disease resistance expression time and the space that disease-resistant spectrum is wide, the persistent period is longer, can control, its
In the most important thing is most of inducer environmentally safe of having now been found that, if the actual application in field, it will in certain journey
Reducing the use of chemical pesticide on degree, to reducing, agriculture is residual, accelerates non-poisonous pesticides and replaces the process of toxic pesticides, and improves ecology
Environment and ecological balance are the most significant.
Lipid, polysaccharide, nucleic acid, protein matter all can induce host plant to produce systemic disease resistance.China at present
Preventing and treating vegetable virus disease biological preparation effective ingredient mainly include lentinan, Portugal's polyene sugar, oligosaccharide element, Ningnanmycin,
Amino-oligosaccharide, matrine etc., these compositions can excite the system of defense of plant self, simultaneously to the duplication of some virus and
Diffusion has certain effect.But carry out the research of biological preparation that prevents and treats currently for TYLCV and application is the most relatively fewer.
Phytosulfokine-α (PSK) is a kind of plant peptide, initially obtains in the culture medium in Germinatus Phragmitis mesophyll cell suspension cultures
Separate to identifying.PSK is by the precursor peptide of about 80 amino acid longs sulfonation and Proteolytic enzyme processing after the translation of tyrosine residue
And produce (Yang etc., " Oryza sativa PSK gene encodes a precursor of phytosulfokine-α,
a sulfated peptide growth factor found in plants”Proceedings of the National
Academy of Sciences of the United States of America, 1999,96:13560-13565).Shear
The PSK modifying after ripening exists in two forms: pentapeptide PSK α ([H-Tyr (SO3H)-Ile-Tyr (the SO3H)-Thr-of sulfonation
Gln-OH]) and the tetrapeptide PSK β ([H-Tyr (SO3H)-Ile-Tyr (SO3H)-Thr-OH]) of C-end truncate
(Matsubayashi Y. etc., " Phytosulfokine, sulfated peptides that induce the
proliferation of single mesophyll cells of Asparagus oficinalis L.。”
Proceedings of the National Academy of Sciences of the United States of
America, 1996,93:7623-7627) it is considered to be a kind of novel " peptide hormone ".
Its structural formula is as follows:
At present, research existing to PSK α shows, its in cell proliferation and differentiation, separate living tissue is formed, selfing is the most affine
Property, organ come off, stomatal movement, be respectively provided with the attribute of plant growth regulating substance during Resistant reaction etc.
(Matsubayashi etc., " Peptide hormones in plants." Annual Reviewof Plant Biology,
2006,57:649-674).
In the research of PSK α involved in plant disease resistance, PSK α is different to the effect of different pathogen.Arabidopsis
The receptor pskr1/pskr2 double-mutant relatively WT lines of plant PSK α is to alternaria Alternariabrassicicola
More sensitive.In contrast, arabidopsis pskr1/pskr2 mutant is but to bacterial leaf spot Pseudomonas
Syringae more resistance (Igarashi etc., " The peptide growth factor, phytosulfokine,
attenuatespattern-triggered immunity." The Plant Journal, 2012,71:194-204).
Summary of the invention
Present invention discover that phytosulfokine-α-α (PSK α) has auxin (IAA) signal path of induction plant, raising is planted
The thing resistance to tomato yellow leaf curl China virus, reduces the generation of aetiolation leaf curl viral disease.
Find based on above, the invention provides phytosulfokine-α-α in improving aetiolation leaf curl viral disease resistance
Application.
Present invention also offers PSK α application in preparation improves the preparation of aetiolation leaf curl viral disease resistance.
Described plant is specifically as follows Fructus Lycopersici esculenti.
PSK α is sulfonation and the Proteolytic enzyme processing after the translation of tyrosine residue of the precursor peptide by about 80 amino acid longs
And the pentapeptide produced；Auxin (IAA) synthesis and signaling genes FZY2, TAA1, the PIN1 etc. improving plant can be induced
Express thus improve the plant resistance to tomato yellow leaf curl China viral disease.
Present invention also offers a kind of preparation improving aetiolation leaf curl viral disease resistance, the effective ingredient of described preparation
For phytosulfokine-α-α.
As preferably, described preparation, in terms of 1L, including: phytosulfokine-α-α 6.5～10.5g；Surfactant 0.02
～0.03L；Water 0.97～0.98L.
Described surfactant can make preparation the moistening of plant surface, disperse, opens up and permeance property is obviously enhanced, and has
Effect reduces after preparation sprays with wind drift, improves ability to resist splash erosion and drug effect, the consumption of minimizing preparation of preparation, extends system
The effect duration of agent.
Described surfactant can use the one in organosilicon, polysorbate60 or Silwet-L77；It is preferably organosilicon, has
Organic silicon surface active agent price is the cheapest, and the most aobvious improving effect on the ductility of preparation, reduction dosage surface tension force
Write, make preparation be more easy to by plant and absorb.
It is further preferred that described preparation, in terms of 1L, including: phytosulfokine-α-α 8.47g；Surfactant 0.025L；Water
0.975L.In the preparation prepared under above-mentioned each component proportion, phytosulfokine-α-α the dispersibility of plant surface, plate-out ability with
And permeability effects is optimal.
Described preparation is prepared by the following method:
(1) phytosulfokine-α-α is first dissolved in a small amount of water, adds water, obtain mixed solution；
(2) in mixed solution, surfactant is added, it is thus achieved that described preparation.
Present invention also offers a kind of method improving aetiolation leaf curl viral disease resistance, including: by claim 4～
Spray after 6 arbitrary described preparation dilute with waters in plant leaf blade surface or drip irrigation near plant root.
Invention formulation can in the environment of continuous high light, high temperature, arid etc. easily send out tomato yellow leaf curl virus disease or
Occur that the period of slight tomato yellow leaf curl China virus disease symptoms uses.
In the concentration of preparation, number of times, especially preparation, the concentration of PSK α can be according to the seedling age of plant, specifically grow feelings
Condition and plant growth environment situation, degree of disease determine.
As preferably, after described preparation diluent, spraying the concentration in the phytosulfokine-α-α on plant leaf blade surface is
67.76～101.64mg/L.
It is an advantage of the current invention that:
(1) present invention has excavated new raising aetiolation leaf curl viral disease resistance for known compound sulphur Copeptin-α
Purposes, has opened up a new application.
(2) the sulphur Copeptin-α in invention formulation is plant friendly material, can degrade in environmental and biological materials, uses
After also noresidue, to people and animals and environment nonhazardous.
(3) preparation that the present invention is prepared with sulphur Copeptin-α for principle active component, by the auxin in induction plant
(IAA) signal path, can be obviously enhanced the plant resistance to tomato yellow leaf curl China viral disease, reduces because tomato yellow leaf curl China viral disease is to planting
The economic loss that strain brings.
(4) use invention formulation preventing and treating aetiolation leaf curl viral disease simple, cost is relatively low, can significantly postpone and
Suppress tomato yellow leaf curl China virus growth on blade and the diffusion of disease, substantially increase plant and tomato yellow leaf curl China viral disease is resisted
Property.
Accompanying drawing explanation
Fig. 1 be in embodiment 1 after tomato yellow leaf curl virus inoculation the 7th day, 14 days, after 21 days, spraying preparation is clear with spray
The comparison of TYLCV relative dna content in Fructus Lycopersici esculenti young leaves between water comparison；
Open tubular column represents adjoining tree, and twill post representative processes plant；Lower case a, b represent between different disposal 5%
Significant difference in level.
Fig. 2 is to spray after preparation carries out tomato yellow leaf curl virus inoculation with the spray plant that compares of clear water in embodiment 2
After process, the comparison that a situation arises of tomato leaf disease；
A is that tomato yellow leaf curl virus inoculates 21 days restrovirus disease sickness rate；B is that tomato yellow leaf curl virus is inoculated 21 days
TYLCV relative dna content in rear blade；
Open tubular column represents adjoining tree, and twill post representative processes plant；Lower case a, b represent between different disposal 5%
Significant difference in level.
Fig. 3 is the field effect of invention formulation in embodiment 3, sprayed preparation and sends out with the spray tomato disease that compares of clear water
Raw rate and the comparison that serious conditions occurs；
A is tomato yellow leaf curl virus disease incidence rate；B is healthy after spraying and do not spray invention formulation and morbidity Fructus Lycopersici esculenti
The Net Photosynthetic Rate (Pn) of blade；Open tubular column represents adjoining tree, and twill post representative processes plant；Lower case a, b, c represent
Significant difference in 5% level between different disposal.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further explained.
Embodiment 1
One, the preparation of preparation
Take sulphur Copeptin-α (PSK α) 2.54g (medicine is purchased from NeoMPS company of France)；It is slowly added to the water of 294.0mL, and
By mixed solution mix homogeneously, the most backward mixed solution adds the organic silicon solution of 6.0mL, after stirring, it is thus achieved that preparation.
Two, the using and detecting of preparation
Take 100 milliliters of above-mentioned preparation, add 10 liters of clear water, after mix homogeneously, the most uniformly spray in seedling age be four leaves one
The tomato leaf surface of the heart, till blade is moistening, persistently sprays 2 days, to spray the tomato plant of clear water as comparison.
To above-mentioned process plant with sterilization bamboo let picking Agrobacterium infectious clone thalline in Fructus Lycopersici esculenti 1-2 sheet true leaf stem stalk
Place's stab inoculation tomato yellow leaf curl virus.All plant of above-mentioned process are placed in 28 DEG C, 70% humidity, 12h illumination/12h
Dark, light intensity is 400 μm ol m-2s-1In the environment of cultivate.
After inoculation, within the 7th day, 14 days, 21 days, take the top tender young leaves of children the most at random, utilize Beijing TIANGEN company to produce
Plant Genomic DNA Kit test kit carry out DNA extraction, after utilize Applied Biosystems company of the U.S. to produce
Step ONE Plus Real-Time PCR System instrument and the SYBR RT-PCR Kit fluorescent dye reagent of the said firm
The method that box carries out fluorescent quantitation qPCR to the relative DNA expression of TYLCV detects, and the TYLCV special primer of employing is:
F:5 '-CTTCAAGGAATTCATGGGGG-3 ', R:5 '-GTCCCCAAGTAGTCTGTCTT-3 '.
Result is as it is shown in figure 1, the plant leaf that above-mentioned preparation processes has significantly stronger than the plant leaf of comparison
TYLCV resistance.After inoculation the 7th day, 14 days, after 21 days, TYLCV in the plant top young leaflet tablet that preparation processed virus
DNA relative amount is respectively the 14.8% of adjoining tree, 27.9% and 39.6%.
Embodiment 2
One, the preparation of preparation
Take sulphur Copeptin-α (PSK α) 2.03g (medicine is purchased from NeoMPS company of France)；It is slowly added to the water of 292.5mL, and
By mixed solution mix homogeneously, the most backward mixed solution adds the organic silicon solution of 7.5mL, after stirring, it is thus achieved that preparation.
Two, the using and detecting of preparation
Take 100 milliliters of above-mentioned preparation, add 10 liters of clear water, after mix homogeneously, the most uniformly spray in seedling age be SANYE one
The tomato leaf surface of the heart, till blade is moistening, persistently sprays 2 days, to spray the tomato plant of clear water as comparison.
To above-mentioned process plant with sterilization bamboo let picking Agrobacterium infectious clone thalline in Fructus Lycopersici esculenti 1-2 sheet true leaf stem stalk
Place's stab inoculation tomato yellow leaf curl virus.All plant of above-mentioned process are placed in 32 DEG C, 70% humidity, 12h illumination/12h
Dark, light intensity is 400 μm ol m-2s-1In the environment of cultivate and observe plant incidence.
In inoculation after the 21st day, tomato yellow leaf curl virus disease sickness rate is added up；And it is tender newly to take top children at random
Leaf, the Plant Genomic DNA Kit test kit utilizing Beijing TIANGEN company to produce carries out DNA extraction, after utilize the U.S.
Step ONE Plus Real-Time PCR System instrument that Applied Biosystems company produces and the said firm
The method that SYBR RT-PCR Kit fluorescent dye test kit carries out fluorescent quantitation qPCR to the relative DNA expression of TYLCV is carried out
Detection, the TYLCV special primer of employing is:
F:5 '-CTTCAAGGAATTCATGGGGG-3 ', R:5 '-GTCCCCAAGTAGTCTGTCTT-3 '.
As shown in figure 2 a andb, after inoculating the 21st day, in adjoining tree, after artificial vaccination, the sickness rate of TYLCV is result
89%, the sickness rate of the tomato yellow leaf curl virus disease after said preparation process then have dropped 52.8%, and blade TYLCV's is relative
DNA expression also have dropped 56.5% than comparison.
Embodiment 3
One, the preparation of preparation
Take sulphur Copeptin-α (PSK α) 3.05g (medicine is purchased from NeoMPS company of France)；It is slowly added to the water of 291.0mL, and
By mixed solution mix homogeneously, the most backward mixed solution adds the organic silicon solution of 9.0mL, after stirring, it is thus achieved that preparation.
Two, the using and detecting of preparation
Choose cultivation technique and manage on all four two adjacent hot houses, in high temperature and little rainfall season at the beginning of the autumn, right
Process canopy and use above-mentioned preparation, take 200 milliliters of preparation, add 20 liters of clear water, after mix homogeneously, in the morning with the most uniform
Spraying in seedling age is, seven leaves tomato leaf surface wholeheartedly moistens to blade, persistently to spray 2 days；And compare canopy and only spray phase
The water of same volume.
After 20 days, random statistical processes canopy and compares the sickness rate of each 60 strain Fructus Lycopersici esculenties in canopy, and by photosynthetic parameters ratio
Relatively process canopy and the health status compareing canopy plant.Photosynthesis rate uses the portable photosynthetic of Li-Cor company of U.S. production
Instrument Li-6400 measures Net Photosynthetic Rate, leaf temperature, light intensity and CO2Concentration is respectively set as 25 DEG C, 800 μm ol m-2s-1、360
μmol mol-1。
Result is as it is shown on figure 3, after the sick phase process invention formulation occurred frequently of tomato yellow leaf curl virus 20 days, hence it is evident that increase
The strong tomato plant resistance to TYLCV, the plant that invention formulation processes have dropped 57.1% than the diseased plant rate of adjoining tree.
Processing plant similar to the Net Photosynthetic Rate (Pn) of healthy plant of not falling ill in adjoining tree, above-mentioned preparation is still fallen ill after processing
Plant also than Net Photosynthetic Rate (Pn) mean height 46.5% of disease plant under the conditions of comparison.
Claims (8)
1. phytosulfokine-α-α application in improving aetiolation leaf curl viral disease resistance.
2. phytosulfokine-α-α application in preparation improves the preparation of aetiolation leaf curl viral disease resistance.
Apply the most as claimed in claim 1 or 2, it is characterised in that described plant is Fructus Lycopersici esculenti.
4. a preparation, it is characterised in that the effective ingredient of described preparation is phytosulfokine-α-α.
5. preparation as claimed in claim 4, it is characterised in that in terms of 1L, including:
Phytosulfokine-α-α 6.5～10.5g；
Surfactant 0.02～0.03L；
Water 0.97～0.98L.
6. preparation as claimed in claim 4, it is characterised in that in terms of 1L, including:
Phytosulfokine-α-α 8.47g；
Surfactant 0.025L；
Water 0.975L.
7. the method improving aetiolation leaf curl viral disease resistance, it is characterised in that including: by arbitrary for claim 4～6
Spray after described preparation dilute with water in plant leaf blade surface or drip irrigation near plant root.
8. method as claimed in claim 7, it is characterised in that after described preparation diluent, spray in plant leaf blade surface
The concentration of phytosulfokine-α-α is 67.76～101.64mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610277808.3A CN105900983B (en) | 2016-04-27 | 2016-04-27 | Applications of the phytosulfokine-α α in aetiolation leaf curl viral disease resistance is improved |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610277808.3A CN105900983B (en) | 2016-04-27 | 2016-04-27 | Applications of the phytosulfokine-α α in aetiolation leaf curl viral disease resistance is improved |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105900983A true CN105900983A (en) | 2016-08-31 |
| CN105900983B CN105900983B (en) | 2018-03-16 |
Family
ID=56752360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610277808.3A Active CN105900983B (en) | 2016-04-27 | 2016-04-27 | Applications of the phytosulfokine-α α in aetiolation leaf curl viral disease resistance is improved |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105900983B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114369A (en) * | 2017-03-24 | 2017-09-01 | 浙江大学 | Applications of the phytosulfokine-α α in plant botrytis resistance is improved |
| CN109374763A (en) * | 2018-10-18 | 2019-02-22 | 中国科学院遗传与发育生物学研究所 | The quantitative detecting method of plant endo phytosulfokine-α content |
| CN109938027A (en) * | 2019-04-03 | 2019-06-28 | 江西鑫邦科技有限责任公司 | A kind of composition pesticide of anti-plant viral disease and its preparation method and application |
| CN112877326A (en) * | 2021-04-06 | 2021-06-01 | 浙江大学 | Application of aluminum ion receptor ALR1 gene or protein for regulating and controlling aluminum resistance of plants |
| CN114752622A (en) * | 2022-05-05 | 2022-07-15 | 安庆市长三角未来产业研究院 | Application of polypeptide receptor PSKR1 gene in improving high-temperature stress resistance of tomato plants and/or tomato pollen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2719763A1 (en) * | 2012-10-10 | 2014-04-16 | VIB, vzw | Heterodimeric transcription factor involved in PSK synthesis |
| CN103976908A (en) * | 2014-05-29 | 2014-08-13 | 暨南大学 | Plant extract composition containing plant polypeptides and application thereof in cosmetics |
-
2016
- 2016-04-27 CN CN201610277808.3A patent/CN105900983B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2719763A1 (en) * | 2012-10-10 | 2014-04-16 | VIB, vzw | Heterodimeric transcription factor involved in PSK synthesis |
| CN103976908A (en) * | 2014-05-29 | 2014-08-13 | 暨南大学 | Plant extract composition containing plant polypeptides and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
|---|
| 陈新建 等: "植物新型肽类生长调节物质——植物磺肽素", 《植物生理学通讯》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114369A (en) * | 2017-03-24 | 2017-09-01 | 浙江大学 | Applications of the phytosulfokine-α α in plant botrytis resistance is improved |
| CN109374763A (en) * | 2018-10-18 | 2019-02-22 | 中国科学院遗传与发育生物学研究所 | The quantitative detecting method of plant endo phytosulfokine-α content |
| CN109938027A (en) * | 2019-04-03 | 2019-06-28 | 江西鑫邦科技有限责任公司 | A kind of composition pesticide of anti-plant viral disease and its preparation method and application |
| CN112877326A (en) * | 2021-04-06 | 2021-06-01 | 浙江大学 | Application of aluminum ion receptor ALR1 gene or protein for regulating and controlling aluminum resistance of plants |
| CN112877326B (en) * | 2021-04-06 | 2022-07-08 | 浙江大学 | Application of aluminum ion receptor ALR1 gene or protein for regulating and controlling aluminum resistance of plants |
| CN114752622A (en) * | 2022-05-05 | 2022-07-15 | 安庆市长三角未来产业研究院 | Application of polypeptide receptor PSKR1 gene in improving high-temperature stress resistance of tomato plants and/or tomato pollen |
| CN114752622B (en) * | 2022-05-05 | 2023-09-01 | 安庆市长三角未来产业研究院 | Application of polypeptide receptor PSKR1 gene in improving high-temperature stress resistance of tomato plants and/or tomato pollen |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105900983B (en) | 2018-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pérez-Jiménez | Significant avocado diseases caused by fungi and oomycetes | |
| CN105900983B (en) | Applications of the phytosulfokine-α α in aetiolation leaf curl viral disease resistance is improved | |
| Follak et al. | Biological flora of central Europe: Cyperus esculentus L. | |
| CN102687730B (en) | Biological compound preparation for controlling plant virus disease and application thereof | |
| Trzewik et al. | The influence of Piriformospora indica on the resistance of two rhododendron cultivars to Phytophthora cinnamomi and P. plurivora | |
| Jambhulkar et al. | Potential native Trichoderma strains against Fusarium verticillioides causing post flowering stalk rot in winter maize | |
| CN104388448B (en) | A kind of corn phospholipase A_2 gene ZmsPLA2-1 and its application | |
| CN107114369B (en) | Application of phytosulfokine-alpha in improving gray mold resistance of plants | |
| Massee | A text-book of plant diseases caused by cryptogamic parasites | |
| Bondarenko et al. | Major cucumber diseases and the crop immunity | |
| CN105028036A (en) | Prevention and control method for pests during grape planting period | |
| CN105900981B (en) | Application of the sub- Oleoyl monoethanolamide in aetiolation leaf curl viral disease resistance is improved | |
| CN105002111B (en) | It is a kind of prevent and treat cucumber downy mildew bacterial strain TDJN1 and its application | |
| Pandey | Plant pathology (Pathogen and Plant disease) | |
| Gurung | Morphological and molecular identification of major fungal disease of large cardamom (Amomum subulatum Roxb.) in Sikkim and its management using botanicals | |
| Sofy et al. | Salix alba extract induces systemic resistance in Cucumis sativus infected by Cucumber mosaic virus | |
| CN106906228A (en) | GhLMM genes and its application of Cotton disease resistance can be significantly improved | |
| Millhollon | Loose kernel smut for biocontrol of Sorghum halepense in Saccharum sp. hybrids | |
| Barriga Medina | Exploring fungal pathogens to control invasive raspberry (Rubus niveus) in Galapagos Islands | |
| Gupta | Text Book of Plant Diseases | |
| Nikolić et al. | Nutritional quality and digestibility of maize hybrid plants for silage. Life sciences in dialogue of generations: connections between universities, academia and business | |
| Ayaa | Infection of Phaseolus vulgaris with the plant pathogen Colletotrichum species: A thesis submitted in partial fulfillment of the requirements for the Degree of Master at Lincoln University | |
| Rashwan et al. | Impact of Certain Eco-Friendly Agents and Safety Commercial Nematicide Compared with The Chemical Nematicide Vydate for Controlling Meloidogyne javanica Infected Tomato Plants | |
| HU202064B (en) | Weed controlling process with utilizing spore culture of phomopsis convolvulus and process for producing the culture the culture of fungi | |
| Abou Shanab et al. | Impact of Certain Eco-Friendly Agents and Safety Commercial Nematicide Compared with The Chemical Nematicide Vydate for Controlling Meloidogyne javanica Infected Tomato Plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |