CN107089881A - A kind of mushroom culture medium using Cortex sophorae as raw material and preparation method thereof - Google Patents

A kind of mushroom culture medium using Cortex sophorae as raw material and preparation method thereof Download PDF

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Publication number
CN107089881A
CN107089881A CN201710496133.6A CN201710496133A CN107089881A CN 107089881 A CN107089881 A CN 107089881A CN 201710496133 A CN201710496133 A CN 201710496133A CN 107089881 A CN107089881 A CN 107089881A
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China
Prior art keywords
parts
raw
culture medium
mushroom
cortex sophorae
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CN201710496133.6A
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Chinese (zh)
Inventor
唐纪梅
杨正文
余世英
吴高齐
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Songtao Miao Autonomous County Farming Cooperatives After Mei
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Songtao Miao Autonomous County Farming Cooperatives After Mei
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Priority to CN201710496133.6A priority Critical patent/CN107089881A/en
Publication of CN107089881A publication Critical patent/CN107089881A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention belongs to mushroom cultivation technical field, especially a kind of mushroom culture medium using Cortex sophorae as raw material and preparation method thereof, using Cortex sophorae, watermelon peel, vinasse, Chinese prickly ash, watermifoil, wheat bran, fertile soil, myotonin, garlic, sucrose, gelatine, microcrystalline cellulose and bentonite are raw material, material source is wide, it is with low cost, and after reasonably arranging in pairs or groups, so that culture medium comprehensive nutrition, water retention property is good, it can also effectively kill germ simultaneously, promote mycelial growth, shorten growth cycle, improve the biological transformation ratio of mushroom, and then improve the yield and quality of mushroom.

Description

A kind of mushroom culture medium using Cortex sophorae as raw material and preparation method thereof
Technical field
The invention belongs to mushroom cultivation technical field, especially a kind of mushroom culture medium and its system using Cortex sophorae as raw material Preparation Method.
Background technology
Mushroom, also known as flower mushroom, Hericium erinaceus, mushroom, fragrant letter, fragrant bacterium, dried mushroom, fragrant wild rice, are that the son of Pleurotaceae plant mushroom is real Body.Mushroom is second-biggest-in-the-world edible mushroom, is also one of China's special product, in the title among the people for have " mountain delicacy ";It is a kind of growth Fungi on timber.Delicious flavour, fragrance make people mentally refreshing is nutritious;Mushroom is rich in vitamin B complex, iron, potassium, provitamin D It is (vitamin D is changed into after Exposure to Sunlight), sweet, it is mild-natured.Anorexia is cured mainly, few gas is weak;Mushroom have the title of " king of mountain delicacy ", It is high protein, low-fat nutritional health food;Chinese dynasties physician has famous discussion to mushroom.Modern medicine and nutrition Deepened continuously research, and the medical value of mushroom is also constantly exploited.Quantitative Determination of Ergosterol is very high in mushroom, to preventing and treating rickets Effectively;Lentinan (β~1,3 glucans) can strengthen cell immunocompetent, so as to suppress the growth of cancer cell;Mushroom contains six More than 40 kinds of enzyme of big enzyme, can correct human chitinase deficiency disease;Fatty institute's fatty acids in mushroom, have to human body reduction blood fat Benefit.Mushroom -- fungi queen.Mushroom, also known as mushroom, dried mushroom, are a kind of Mycophytas being grown on timber;Due to its taste compared with Perfume (or spice), pleasant aroma is nutritious, not only ranks straw mushroom, flat mushroom, on white mushroom, and have the reputation of " fungi queen ".
Traditional edible mushroom solid culture based raw material especially nitrogen source can often be used as feedstuff, cost compared with It is high;And the culture medium raw material quantity for producing edible mushroom consumption is very big, this causes edible mushroom cost to remain high, in world food and Under the background that feed price all constantly rises, using agricultural wastes or agricultural product accessory substance through reasonable combination and fortification Prepare high-quality, safe, inexpensive edible mushroom solid medium turns into the focus of research.
The content of the invention
In order to solve the above technical problems, the present invention provides a kind of mushroom culture medium using Cortex sophorae as raw material.
It is achieved particular by following technical scheme:
A kind of mushroom culture medium using Cortex sophorae as raw material, raw material is 80~100 parts of Cortex sophorae, watermelon peel in parts by weight 50~60 parts, 30~40 parts of vinasse, 15~20 parts of Chinese prickly ash, 10~15 parts of watermifoil, 14~18 parts of wheat bran, fertile soil 12~ 16 parts, 5~10 parts of myotonin, 1~3 part of garlic, 2~4 parts of sucrose, 6~9 parts of gelatine, 2~5 parts of microcrystalline cellulose, bentonite 4 ~7 parts.
Further preferred raw material is in parts by weight 92 parts of Cortex sophorae, 54 parts of watermelon peel, 38 parts of vinasse, Chinese prickly ash are sub 17 parts, It is 14 parts of watermifoil, 16 parts of wheat bran, 15 parts of fertile soil, 130~150 parts of myotonin, 2 parts of garlic, 3 parts of sucrose, 7 parts of gelatine, micro- 4 parts of crystalline cellulose, 5 parts of bentonite.
The culture medium use condition is 600~700lx in intensity of illumination, and temperature is 18~21 DEG C.
It is a further object to provide the preparation method of the mushroom culture medium, comprise the following steps:
(1) Cortex sophorae, vinasse, Chinese prickly ash, watermifoil, wheat bran, myotonin and garlic are put into concentration for 2~5% salt 12~16h is soaked in water, ultramicro grinding is carried out after mixing;
(2) after mixing sucrose, microcrystalline cellulose and gelatine, add ionized water and be adjusted to slurries;By watermelon peel, bentonite After being mixed with fertile soil, it is added to after being crushed to 100~200 mesh in slurries, 10 is handled using 100~150r/min mixers ~20min;
(3) step (1), step (2) resulting material are mixed, add ionized water stir to moisture reach 65~ 75%, and pH value is adjusted, most afterwards through high-temperature sterilization.
Watermelon peel water at a temperature of 120~140 DEG C boils 30~40min, and filtrate is obtained after filtering.
After step (3) material is mixed, using 15~20KHz 25~30s of ultrasonication, temperature is 35~40 ℃。
The regulation p H values are using any in sodium hydroxide, calcium hydroxide, potassium hydroxide.
Beneficial effect of the present invention:
Chinese scholartree as economic forest trim discarded object usually by when bavin standing grain burn-up or not available discarded object lose, therefore this Invention Cortex sophorae is raw material, has expanded the available sources of culture medium raw material, is coordinating other compositions reasonably to be arranged in pairs or groups, is being planted The single rate of the mushroom of training out is big, and the average single rate of each 1000mL culture bottle is 350g;The present invention uses watermelon peel It is special because it contains the materials such as abundant protein, sugar, acid, nitrogen, tannin, amino acid and other trace elements as raw material It is not, also containing citrulling, and then the nutrition species of rich medium is enriched, to improve the resistance against diseases of mushroom, accelerate the life of mushroom Long development;In the suitable illumination of cooperation with a temperature of, it is possible to increase accelerate the growth of fructification, while improving flatness and effective Mushroom flower bud number, thickening stem, and then improve yield;The bentonite of addition has the effect of absorption harmful heavy metal power, and then prevents Influence of the heavy metal to mushroom quality.
The present invention using Cortex sophorae, watermelon peel, vinasse, Chinese prickly ash, watermifoil, wheat bran, fertile soil, myotonin, garlic, Sucrose, gelatine, microcrystalline cellulose and bentonite are raw material, and material source is wide, with low cost, and by reasonably arranging in pairs or groups after, So that culture medium comprehensive nutrition, water retention property is good, while germ can also effectively be killed, promote mycelial growth, shorten growth week Phase, the biological transformation ratio of mushroom is improved, and then improve the yield and quality of mushroom.
Embodiment
Tuberculosis specific embodiment is limited technical scheme is further below, but claimed Scope is not only limited to made description.
Embodiment 1
Formula:Cortex sophorae 80kg, watermelon peel 50kg, vinasse 30kg, the sub- 15kg of Chinese prickly ash, watermifoil 10kg, wheat bran 14kg, Fertile soil 12kg, myotonin 5kg, garlic 1kg, sucrose 2kg, gelatine 6kg, microcrystalline cellulose 2kg, bentonite 4kg.
Preparation method:(1) Cortex sophorae, vinasse, Chinese prickly ash, watermifoil, wheat bran, myotonin and garlic are put into concentration is Soaked in 2% salt solution and ultramicro grinding is carried out after 12h, mixing;
(2) after mixing sucrose, microcrystalline cellulose and gelatine, add ionized water and be adjusted to slurries;By watermelon peel, bentonite After being mixed with fertile soil, it is added to after being crushed to 100 mesh in slurries, 10min is handled using 100r/min mixers;
(3) step (1), step (2) resulting material are mixed, adds ionized water and stir to moisture and reach 65%, and PH value is adjusted, most afterwards through high-temperature sterilization.
The culture medium use condition is 600lx in intensity of illumination, and temperature is 18 DEG C.
Watermelon peel water at a temperature of 120 DEG C boils 30min, and filtrate is obtained after filtering.
After step (3) material is mixed, using 15KHz ultrasonication 25s, temperature is 35 DEG C.
The regulation p H values use sodium hydroxide.
Test effect is verified:Test group uses the medium culture mushroom of embodiment 1, and control group uses conventional medium culture The growing state of mushroom is observed in mushroom, incubation, until after mushroom is ripe, to mushroom single bacterium weight, mycoderm thickness, total yield Amount, biological transformation ratio and sell price and measure and count that (wherein single bacterium weight is with mycoderm method for measuring thickness:Into 100 are randomly selected in ripe experiment mushroom to average), as a result such as following table:
As seen from the experiment, the single bacterium weight of test group is apparently higher than control group, and mycoderm thickness is higher than control group, and The body growing way of mycelia is denser than control group, and price is higher than control group, and yield is higher than control group, and biological transformation ratio reaches 112.7%.
Embodiment 2
Formula:Cortex sophorae 100kg, watermelon peel 60kg, vinasse 40kg, the sub- 20kg of Chinese prickly ash, watermifoil 15kg, wheat bran 18kg, fertile soil 16kg, myotonin 10kg, garlic 3kg, 4 parts of sucrose, gelatine 9kg, microcrystalline cellulose 5kg, bentonite 7kg.
Preparation method:(1) Cortex sophorae, vinasse, Chinese prickly ash, watermifoil, wheat bran, myotonin and garlic are put into concentration is Soaked in 5% salt solution and ultramicro grinding is carried out after 16h, mixing;
(2) after mixing sucrose, microcrystalline cellulose and gelatine, add ionized water and be adjusted to slurries;By watermelon peel, bentonite After being mixed with fertile soil, it is added to after being crushed to 200 mesh in slurries, 20min is handled using 150r/min mixers;
(3) step (1), step (2) resulting material are mixed, adds ionized water and stir to moisture and reach 75%, and PH value is adjusted, most afterwards through high-temperature sterilization.
The culture medium use condition is 700lx in intensity of illumination, and temperature is 21 DEG C.
Watermelon peel water at a temperature of 140 DEG C boils 40min, and filtrate is obtained after filtering.
After step (3) material is mixed, using 20KHz ultrasonication 30s, temperature is 40 DEG C.
The regulation p H values use calcium hydroxide.
Embodiment 3
Formula:Cortex sophorae 92kg, watermelon peel 54kg, vinasse 38kg, the sub- 17kg of Chinese prickly ash, watermifoil 14kg, wheat bran 16kg, Fertile soil 15kg, 130~150kg of myotonin, garlic 2kg, sucrose 3kg, gelatine 7kg, microcrystalline cellulose 4kg, bentonite 5kg.
Preparation method:(1) Cortex sophorae, vinasse, Chinese prickly ash, watermifoil, wheat bran, myotonin and garlic are put into concentration is Soaked in 3% salt solution and ultramicro grinding is carried out after 15h, mixing;
(2) after mixing sucrose, microcrystalline cellulose and gelatine, add ionized water and be adjusted to slurries;By watermelon peel, bentonite After being mixed with fertile soil, it is added to after being crushed to 170 mesh in slurries, 15min is handled using 130r/min mixers;
(3) step (1), step (2) resulting material are mixed, adds ionized water and stir to moisture and reach 72%, and PH value is adjusted, most afterwards through high-temperature sterilization.
The culture medium use condition is 640lx in intensity of illumination, and temperature is 19 DEG C.
Watermelon peel water at a temperature of 130 DEG C boils 35min, and filtrate is obtained after filtering.
After step (3) material is mixed, using 18KHz ultrasonication 27s, temperature is 37 DEG C.
The regulation p H values use potassium hydroxide.
Embodiment 4`
Formula:80 parts of Cortex sophorae, 60 parts of watermelon peel, 30 parts of vinasse, Chinese prickly ash 20 parts, 10 parts of watermifoil, 14 parts of wheat bran, 16 parts of fertile soil, 5 parts of myotonin, 1 part of garlic, 2 parts of sucrose, 7 parts of gelatine, 3 parts of microcrystalline cellulose, 4 parts of bentonite.
Preparation method:(1) Cortex sophorae, vinasse, Chinese prickly ash, watermifoil, wheat bran, myotonin and garlic are put into concentration is Soaked in 3% salt solution and ultramicro grinding is carried out after 15h, mixing;
(2) after mixing sucrose, microcrystalline cellulose and gelatine, add ionized water and be adjusted to slurries;By watermelon peel, bentonite After being mixed with fertile soil, it is added to after being crushed to 180 mesh in slurries, 18min is handled using 140r/min mixers;
(3) step (1), step (2) resulting material are mixed, adds ionized water and stir to moisture and reach 68%, and PH value is adjusted, most afterwards through high-temperature sterilization.
The culture medium use condition is 620lx in intensity of illumination, and temperature is 19 DEG C.
Watermelon peel water at a temperature of 125 DEG C boils 34min, and filtrate is obtained after filtering.
After step (3) material is mixed, using 18KHz ultrasonication 30s, temperature is 38 DEG C.
The regulation p H values use sodium hydroxide.
Embodiment 5
Formula:100 parts of Cortex sophorae, 50 parts of watermelon peel, 30 parts of vinasse, 15 parts of Chinese prickly ash, 15 parts of watermifoil, wheat bran 18 Part, 16 parts of fertile soil, 8 parts of myotonin, 1 part of garlic, 4 parts of sucrose, 7 parts of gelatine, 3 parts of microcrystalline cellulose, 5 parts of bentonite.
Preparation method:(1) Cortex sophorae, vinasse, Chinese prickly ash, watermifoil, wheat bran, myotonin and garlic are put into concentration is Soaked in 4% salt solution and ultramicro grinding is carried out after 15h, mixing;
(2) after mixing sucrose, microcrystalline cellulose and gelatine, add ionized water and be adjusted to slurries;By watermelon peel, bentonite After being mixed with fertile soil, it is added to after being crushed to 120 mesh in slurries, 20min is handled using 110r/min mixers;
(3) step (1), step (2) resulting material are mixed, adds ionized water and stir to moisture and reach 65%, and PH value is adjusted, most afterwards through high-temperature sterilization.
The culture medium use condition is 660lx in intensity of illumination, and temperature is 20 DEG C.
Watermelon peel water at a temperature of 135 DEG C boils 38min, and filtrate is obtained after filtering.
After step (3) material is mixed, using 19KHz ultrasonication 26s, temperature is 36 DEG C.
The regulation p H values use calcium hydroxide.

Claims (7)

1. a kind of mushroom culture medium using Cortex sophorae as raw material, it is characterised in that raw material is Cortex sophorae 80~100 in parts by weight Part, 50~60 parts of watermelon peel, 30~40 parts of vinasse, 15~20 parts of Chinese prickly ash, 10~15 parts of watermifoil, 14~18 parts of wheat bran, 12~16 parts of fertile soil, 5~10 parts of myotonin, 1~3 part of garlic, 2~4 parts of sucrose, 6~9 parts of gelatine, microcrystalline cellulose 2~5 Part, 4~7 parts of bentonite.
2. as claimed in claim 1 using Cortex sophorae as the mushroom culture medium of raw material, it is characterised in that the raw material is in parts by weight For 92 parts of Cortex sophorae, 54 parts of watermelon peel, 38 parts of vinasse, Chinese prickly ash 17 parts, 14 parts of watermifoil, 16 parts of wheat bran, 15 parts of fertile soil, 130~150 parts of myotonin, 2 parts of garlic, 3 parts of sucrose, 7 parts of gelatine, 4 parts of microcrystalline cellulose, 5 parts of bentonite.
3. as claimed in claim 1 or 2 using Cortex sophorae as the mushroom culture medium of raw material, it is characterised in that the culture medium is used Condition is 600~700lx in intensity of illumination, and temperature is 18~21 DEG C.
4. as claimed in claim 1 using Cortex sophorae as the preparation method of the mushroom culture medium of raw material, it is characterised in that including following Step:
(1) Cortex sophorae, vinasse, Chinese prickly ash, watermifoil, wheat bran, myotonin and garlic are put into concentration in 2~5% salt solution 12~16h is soaked, ultramicro grinding is carried out after mixing;
(2) after mixing sucrose, microcrystalline cellulose and gelatine, add ionized water and be adjusted to slurries;By watermelon peel, bentonite and corruption Grow soil mixing after, be added to after being crushed to 100~200 mesh in slurries, using 100~150r/min mixers processing 10~ 20min;
(3) step (1), step (2) resulting material are mixed, adds ionized water and stir to moisture and reach 65~75%, and PH value is adjusted, most afterwards through high-temperature sterilization.
5. as claimed in claim 4 using Cortex sophorae as the preparation method of the mushroom culture medium of raw material, it is characterised in that the watermelon Skin water at a temperature of 120~140 DEG C boils 30~40min, and filtrate is obtained after filtering.
6. as claimed in claim 4 using Cortex sophorae as the preparation method of the mushroom culture medium of raw material, it is characterised in that the step (3) after material is mixed, using 15~20KHz 25~30s of ultrasonication, temperature is 35~40 DEG C.
7. as claimed in claim 4 using Cortex sophorae as the preparation method of the mushroom culture medium of raw material, it is characterised in that the regulation PH value uses any in sodium hydroxide, calcium hydroxide, potassium hydroxide.
CN201710496133.6A 2017-06-26 2017-06-26 A kind of mushroom culture medium using Cortex sophorae as raw material and preparation method thereof Pending CN107089881A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109429902A (en) * 2018-12-18 2019-03-08 苏思晓 Improve the preparation method of mushroom Quantitative Determination of Ergosterol barrel

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