CN107058529A - A kind of method of seed selection stripe rust of wheat durable resistance material - Google Patents
A kind of method of seed selection stripe rust of wheat durable resistance material Download PDFInfo
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Abstract
The invention belongs to wheat breeding for disease resistance field, specifically disclose a kind of using the identification of mixing microspecies high pressure and many for baclccrossing techniques, the Adult plant disease-resistant genes such as seedling stage disease-resistant gene and Yr18 are combined, high effect culture obtains the selection of stripe rust durable resistance breeding material.This method can provide excellent intermediate materials for rust-proofing breeding.
Description
Technical field
The invention belongs to wheat breeding for disease resistance field, and in particular to a kind of side of seed selection stripe rust of wheat durable resistance material
Method.
Background technology
Stripe rust of wheat is by caused by wheat bar shaped handle rest fungus (Puccinia striiformis f.sp.tritici)
Fungoid aeroborne disease, can cause the regional underproduction 0.1-5% of wheat, the underproduction reaches 5-25% when serious.China is in the world most
Big Epidemics of Wheat Strip Rust area, once repeatedly outburst stripe rust was very popular, according to statistics, and 2002-2012 China stripe rust is averaged
The hm of occurring area about 4,200,0002In/year, grave danger is constituted to Wheat Production.Wheat stripe rust uredospore can carry out long distance with air-flow
From the characteristic of propagation, the disease area circulation that collapses can be caused popular rapidly, Shu great areas prevalence disease.
It is the mode for controlling stripe rust most economical and environmentally friendly using Stripe Rust Resistance Gene breeding resistant variety.Based on to wheat
The Resistant reaction of stripe rust, wheat stripe rust resistance genes are divided into two classes：Time of infertility resistance (seedling resistance) and Adult plant are anti-
Property.Time of infertility resistance (seedling resistance) typically lasts for whole growing stage, and the resistant gene has Race specificity, resistance water
Flat height, it is easy to observe, category qualitative character heredity.And Adult plant disease-resistant gene typically has certain resistance to extensive pathogen,
Resistance level is low, is difficult observation, belongs to Inheritance of Quantitative Characters.Because in stripe rust of wheat breeding, breeder is more prone to make
With resistance level it is high, be easy to the time of infertility resistant gene of observation, huge selection pressure is applied with to wheat stripe rust, causes bar
The variation of rest fungus dominant race is fast, novel physiological microspecies easily occur, and this is cause wheat stripe rust resistance rapid loss main
Reason.Exploitation resistance is lasting and does not have the strain-forming period resistance gene of Race specificity, is a Changzhi for controlling stripe rust
Effective measures.But problems faced is that the resistance that single or a small number of strain-forming period resistance genes or QTL are provided does not reach in production
Requirement to wheat breed resistance level.
The content of the invention
The purpose of the present invention includes：
A kind of breeding method for solving seedling resistance and strain-forming period resistance gene individually with limitation is provided；
The method that a kind of lasting, the stable breeding material of seed selection wheat stripe rust resistance is provided.
Specifically, the invention provides a kind of method of seed selection stripe rust of wheat durable resistance material, comprising the following steps：
1) stripe rust of wheat strain is collected, inoculated identification filters out time of infertility resistant material P1；
2) with P1For female parent, with Adult plant containing Yr18 and anti-section bar material P2For male parent, configuration hybridization obtains cenospecies F1；
3) with F1For female parent, with P1For recurrent parent, many generation backcrossings are carried out；From BC1F1To BC5F1, per before generation backcrossing, first select
The time of infertility disease-resistant strain in backcross progeny is selected, winning single-strain blade before heading carries DNA, it is common using Yr18 Adult plants and anti-type
Dominant marker is detected, it is ensured that selected maternal containing Yr18 Adult plants and anti-type gene, to the material for obtaining resistance.
It is preferred that, step 1) the stripe rust of wheat strain be CYR29, CYR30, CYR31, CYR32, CYR33, V26 and/
Or water source 14, it is described to be accredited as the identification of mixed at room temperature microspecies high pressure；
Step 1) inoculation inoculation material be 10P4-8,13P2-7 and/or 14P532.
It is preferred that, step 2) Adult plant containing Yr18 and anti-type male parent material be 13KG458 and/or 13KG662.
It is preferred that, step 3) it is described backcrossing be using the summer numerous added-generation technology backcrossing；
Step 3) the Yr18 Adult plants and anti-type codominance STS be labeled as csLv34；
Step 3) described in detection method be Markers for Detection, wherein PCR amplification system totally 20 μ L, including 2 μ L10 ×
Buffer, 1UTaq archaeal dna polymerase, 0.4 μ L dNTPs, 0.5 μ L primers, 50ng template DNAs, wherein 4 kinds of dNTP concentration is each
For 10mmol L–1, the concentration of Taq archaeal dna polymerases is 2.5U μ L–1, the concentration of forward and reverse primer is 4 μm of ol L -1；PCR
Amplification program is：94 DEG C of denaturation 1min, 57 DEG C of annealing 1min, 72 DEG C of extension 2min, 5 circulations；94 DEG C of denaturation 30s, 57 DEG C are moved back
Fiery 30s, 72 DEG C of extension 1min, 30 circulations；Last 94 DEG C of denaturation 1min, 57 DEG C of annealing 30s, 72 DEG C of extension 5min；
The sequence of the primer is：
Forward primer：5′-GTTGGTTAAGACTGGTGATGG-3′；
Reverse primer：5′-TGCTTGCTATTGCTGAATAGT-3′.
Further, by step 3) pcr amplification product carries out electrophoresis detection, 150bp band occurs in amplified production, then
Material contains Yr18 Adult plants and anti-type gene.
The material selected the invention also discloses any of the above method participates in strain as the sick strain of wheat stripe rust resisting
Comparative experiments or the purposes as breeding intermediate materials.
The nucleotide sequence of the Yr18 Adult plants and anti-type gene, reference can be made to document:Krattinger S G,
Lagudah E S,Spielmeyer W,Singh R P,Huerta-Espino J,McFadden H,Bossolini E,
Selter L L,Keller B.A putative ABC transporter confers durable resistance to
multiple fungal pathogens in wheat.Science,2009,323:1360–1363
The Yr18 Adult plants and anti-type codominance STS mark csLv34, reference can be made to document：Lagudah E S,
McFadden H,Singh R P,Huerta-Espino J,Bariana H S,Spielmeyer W.Molecular
genetic characterization of the Lr34/Yr18slow rusting resistance gene region
in wheat.Theor Appl Genet,2006,114:21–30.Beneficial effect
The inventive method using mixing microspecies high pressure identification and many for baclccrossing techniques, by seedling stage disease-resistant gene and Yr18 etc. into
Strain the phase disease-resistant gene combine, can high effect culture obtain stripe rust durable resistance breeding material, provide excellent centre for rust-proofing breeding
Material.
Brief description of the drawings
Fig. 1 stripe rust of wheat durable resistance high efficiency selected techniqueflow charts
Fig. 2 polyacrylamide gel electrophoresis testing result figures
Wherein, ☆ symbols represent that individual plant contains target gene, may be selected to do backcrossing female parent.
Embodiment
Embodiment one, mixed at room temperature microspecies high pressure identification selection time of infertility resistant material P1Experiment.
By strain mixing for standby use such as collected CYR29, CYR30, CYR31, CYR32, CYR33, V26, water sources 14, in temperature
Material to be screened is planted using potted plant mode in room, each material sowing 5-8 is to be grown to the leaf phase of 1 heart 1, using semar technique
Mixed bacteria is inoculated with, humidifier humidifies 24h is used after inoculation, dark condition is kept, room temperature is controlled at 10-16 DEG C, afterwards regulation room
Temperature is to 20 DEG C or so, and light application time 12h, 15-20d after inoculation after educating 12 abundant morbidities after susceptible control river, identify detected materials
Seedling restance, filter out the good elite clone of the seedling restances such as 10P4-8,13P2-7,14P532.
Embodiment two, P1×P2Hybridization obtains F1 generation experiment.
The elite clone (10P4-8,13P2-7,14P532 etc.) filtered out with embodiment 1 is female parent P1, contained with known
Yr18 Adult plants and anti-section bar material (such as 13KG458,13KG662) are male parent P2, cross combination configuration is carried out, F1 materials are obtained
Material.
Embodiment three, backcrossing experiment and field test
The F1 materials obtained using embodiment two are female parent, simultaneous anti-with the same Adult plant containing Yr18 described in embodiment two
Section bar material (such as 13KG458,13KG662) P2For male parent, hybridization obtains BC1F1Germplasm；The next generation is again with BC1F1For female parent, with
P2For male parent, hybridization obtains BC2F1；In this way, until BC6F1, obtain disease-resistant objective trait and the stable material of economical character
YrP.Above implementation process is carried out under many microspecies induced conditions of field advantage, selected backcrossing maternal individual plant seedling stage and heading
Bar rust resistant phenotype is high anti-afterwards.Inducing materials plantation alternate with backcrossing test material, respectively at the leaf phase of 1 heart 1,3 leaf phases and
Using semar technique inoculation mixing dominant races before heading, 20-30d is carried out after seedling stage assay is inoculated with first, and Adult plant identification exists
Identified after heading.Positive season carries out in Chengdu new capital experimental plot from generation to generation, and Wheat summer sowing adds generation to be carried out in Mt. Luoji.
Example IV, the time of infertility disease-resistant strain Yr18 Adult plants of backcross progeny and the experiment of anti-type identified for genes.
DNA is first extracted before the hybridization of the selected female parent material of embodiment three, is carried out using codominance STS marks csLv34
Yr18 Adult plants and anti-type genescreen, PCR reaction systems totally 20 μ L, including 2 μ L10 × buffer, 1UTaq archaeal dna polymerase
(2.5U μ L -1), 0.4 μ L dNTPs (4 kinds of dNTP concentration is 10mmol L -1), 0.5 μ L primers (4 μm of ol L -1), 50ng
Template DNA.PCR programs are 94 DEG C of denaturation 1min, 57 DEG C of annealing 1min, 72 DEG C of extension 2min, 5 circulations；94 DEG C of denaturation 30s,
57 DEG C of annealing 30s, 72 DEG C of extension 1min, 30 circulations；Last 94 DEG C of denaturation 1min, 57 DEG C of annealing 30s, 72 DEG C of extension 5min.
Pcr amplification product is detected by 6% denaturing polyacrylamide gel electrophoresis, it is contemplated that amplified production is 150bp (bases containing purpose
Cause) and 229bp (being free of target gene).The individual plant that selection is 150bp containing amplified production is that the backcrossing in embodiment three is maternal.
The detection experiment of 6% denaturing polyacrylamide gel electrophoresis：
1st, glass plate prepares：It is flat board by one piece and one piece is that concave-board is fully rinsed well with clear water, then uses anhydrous second
Alcohol is cleaned one time, and then ventilation is dried.
2nd, coated plate：After alcohol is dry, uniform smear peels off silane on concave-board, dries；It is uniform on flat board to smear parent
With silane (affine silane stoste 8ul+ ice ethanol 8ul+ absolute ethyl alcohol 2ml), dry.
3rd, plate is filled：Glass plate is under, and electrophoresis frid is in both sides upper, that edge strip is placed between 2 plates, after alignment, with orchid to folder
Son is by ripple plate and electrophoresis tank board clamping, horizontal positioned.
4th, encapsulating；The 50mL 6% cruel amine gel solutions of poly- third Parties are measured, 350ulAPS (i.e. 10% persulfuric acid ingots are added
And 28ulTEMED), pour into encapsulating device and mix, it is slow uniformly to pour into glue from encapsulating mouthful, then the flat face of comb is inserted
In glue inlet, aeration is careful not to, is placed 1 hour or so.
5th, prerunning：Clip is removed, extracts slotting comb, offset plate is put into electrophoretic, clamping pours into buffer solution, i.e.,
IxTBE.80W weighs power electrophoresis half an hour.
6th, point sample：Comb is extracted, the scrap rubber of encapsulating mouthful is blown clean with liquid-transfering gun, by comb along insertion encapsulating mouthful, then with shifting
Liquid rifle blows clean each comb hole, starts point sample.
7th, electrophoresis：80W weighing apparatus power electrophoresis 1 hour or so.
8th, silver staining：Offset plate is removed from electrophoresis tank, two glass plates is separated, the glass plate glue surface with glue is put into silver upward
15min is contaminated, silver staining process needs lucifuge.
9th, wash：Glass plate is taken out from silver staining liquid, 15s is quickly washed with chain water is steamed.
10th, develop：The glass plate washed is put into developer solution, until band is clear.
11st, wash again：Flushing is gently connect with running water for a moment, the NaOH in glue surface is washed off, placement is dried.
12nd, statistical observation：Glass plate is placed on viewbox the juice strip data that can directly unite；
The gel detection result of part backcross progeny, as shown in Figure of description 2.
Sequence table
<110>Crops Inst., Sichuan Provincial Agricultural Science Academy
<120>A kind of method of seed selection stripe rust of wheat durable resistance material
<130> 2017
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 21
<212> DNA
<213>Artificial sequence
<400> 1
gttggttaag actggtgatg g 21
<210> 2
<211> 21
<212> DNA
<213>Artificial sequence
<400> 2
tgcttgctat tgctgaatag t 21
Claims (9)
1. a kind of method of seed selection stripe rust of wheat durable resistance material, comprises the following steps：
1) stripe rust of wheat strain is collected, inoculated identification filters out time of infertility resistant material P1；
2) with P1For female parent, with Adult plant containing Yr18 and anti-section bar material P2For male parent, configuration hybridization obtains cenospecies F1；
3) with F1For female parent, with P1For recurrent parent, many generation backcrossings are carried out；From BC1F1To BC5F1, per before generation backcrossing, first select back
The time of infertility disease-resistant strain in offspring is handed over, winning single-strain blade before heading carries DNA, using Yr18 Adult plants and anti-type codominance
Mark is detected, it is ensured that selected maternal containing Yr18 Adult plants and anti-type gene, to the material for obtaining resistance.
2. according to the method described in claim 1, it is characterised in that step 1) the stripe rust of wheat strain be CYR29,
CYR30, CYR31, CYR32, CYR33, V26 and/or water source 14, it is described to be accredited as the identification of mixed at room temperature microspecies high pressure.
3. method according to claim 2, it is characterised in that step 1) inoculation material of the inoculation is 10P4-8,
13P2-7 and/or 14P532.
4. method according to claim 3, it is characterised in that step 2) Adult plant containing Yr18 and anti-type male parent material
For 13KG458 and/or 13KG662.
5. method according to claim 4, it is characterised in that step 3) backcrossing is to be returned using summer numerous added-generation technology
Hand over.
6. method according to claim 5, it is characterised in that step 3) the Yr18 Adult plants and anti-type codominance STS
Labeled as csLv34.
7. method according to claim 6, it is characterised in that step 3) described in detection method be Markers for Detection,
Wherein PCR amplification system totally 20 μ L, including 2 μ L10 × buffer, 1UTaq archaeal dna polymerase, 0.4 μ L dNTPs, 0.5 μ L draw
Thing, 50ng template DNAs, wherein 4 kinds of dNTP concentration is respectively 10mmol L–1, the concentration of Taq archaeal dna polymerases is 2.5U μ L–1,
The concentration of forward and reverse primer is 4 μm of ol L–1；PCR amplification programs are：94 DEG C of denaturation 1min, 57 DEG C of annealing 1min, 72 DEG C are prolonged
Stretch 2min, 5 circulations；94 DEG C of denaturation 30s, 57 DEG C of annealing 30s, 72 DEG C of extension 1min, 30 circulations；Last 94 DEG C of denaturation
1min, 57 DEG C of annealing 30s, 72 DEG C of extension 5min；
The sequence of the primer is：
Forward primer：5′-GTTGGTTAAGACTGGTGATGG-3′；
Reverse primer：5′-TGCTTGCTATTGCTGAATAGT-3′.
8. method according to claim 7, it is characterised in that step 3) it is described be detected as, pcr amplification product is subjected to electrophoresis
There is 150bp band in detection, amplified production, then material contains Yr18 Adult plants and anti-type gene.
9. the material that any one of claim 1-8 method is selected as the sick strain of wheat stripe rust resisting participate in strain comparative experiments or
It is used as the purposes of breeding intermediate materials.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107586868A (en) * | 2017-09-11 | 2018-01-16 | 四川农业大学 | With from the mutually chain molecular labeling in wheatgrass P genome Introgressed line Adult plant stripe rust resistances site and its application |
| CN110663542A (en) * | 2019-11-19 | 2020-01-10 | 中国科学院西北高原生物研究所 | Breeding method for long-acting resistance of wheat stem rust Ug99 |
| CN112704005A (en) * | 2020-12-29 | 2021-04-27 | 石河子大学 | Rapid directional improvement technology for wheat target character |
| CN114600767A (en) * | 2022-04-07 | 2022-06-10 | 四川省农业科学院作物研究所 | Method for rapidly breeding wheat durable stripe rust resistant variety |
| CN114731947A (en) * | 2022-05-20 | 2022-07-12 | 四川省农业科学院作物研究所 | Breeding method of novel micro-effect polygene durable disease-resistant wheat variety |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558735A (en) * | 2009-06-03 | 2009-10-21 | 西北农林科技大学 | Novel method for cultivating wheat stripe rust resistant polygene polymerized flower |
| CN103931484A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of widely-resistive wheat variety |
| US20150181875A1 (en) * | 2013-12-31 | 2015-07-02 | Dow Agrosciences Llc | Synergistic fungicidal mixtures for fungal control in cereals |
-
2017
- 2017-03-23 CN CN201710179548.0A patent/CN107058529B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558735A (en) * | 2009-06-03 | 2009-10-21 | 西北农林科技大学 | Novel method for cultivating wheat stripe rust resistant polygene polymerized flower |
| US20150181875A1 (en) * | 2013-12-31 | 2015-07-02 | Dow Agrosciences Llc | Synergistic fungicidal mixtures for fungal control in cereals |
| CN103931484A (en) * | 2014-04-04 | 2014-07-23 | 福建农林大学 | Breeding method of widely-resistive wheat variety |
Non-Patent Citations (1)
| Title |
|---|
| 刘金栋等: ""兼抗型成株抗性小麦品系的培育、鉴定与分子检测"", 《作物学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107586868A (en) * | 2017-09-11 | 2018-01-16 | 四川农业大学 | With from the mutually chain molecular labeling in wheatgrass P genome Introgressed line Adult plant stripe rust resistances site and its application |
| CN107586868B (en) * | 2017-09-11 | 2021-01-15 | 四川农业大学 | Molecular marker linked with stripe rust resistance locus from introgression line of wheatgrass P genome in adult stage and application thereof |
| CN110663542A (en) * | 2019-11-19 | 2020-01-10 | 中国科学院西北高原生物研究所 | Breeding method for long-acting resistance of wheat stem rust Ug99 |
| CN112704005A (en) * | 2020-12-29 | 2021-04-27 | 石河子大学 | Rapid directional improvement technology for wheat target character |
| CN114600767A (en) * | 2022-04-07 | 2022-06-10 | 四川省农业科学院作物研究所 | Method for rapidly breeding wheat durable stripe rust resistant variety |
| CN114731947A (en) * | 2022-05-20 | 2022-07-12 | 四川省农业科学院作物研究所 | Breeding method of novel micro-effect polygene durable disease-resistant wheat variety |
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|---|---|
| CN107058529B (en) | 2022-01-25 |
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