CN107326070A - The special codominant marker of one haynaldia villosa 4VL chromosome and its primer and purposes - Google Patents
The special codominant marker of one haynaldia villosa 4VL chromosome and its primer and purposes Download PDFInfo
- Publication number
- CN107326070A CN107326070A CN201710473068.5A CN201710473068A CN107326070A CN 107326070 A CN107326070 A CN 107326070A CN 201710473068 A CN201710473068 A CN 201710473068A CN 107326070 A CN107326070 A CN 107326070A
- Authority
- CN
- China
- Prior art keywords
- primer
- haynaldia villosa
- chromosomes
- cinau321
- molecular marker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses the special codominant marker of a haynaldia villosa 4VL chromosomes and its primer and purposes, belong to biological technical field.The molecular labeling CINAU321 of haynaldia villosa 4VL chromosomes can be followed the trail of by disclosing first.In the material containing haynaldia villosa 4VL chromosomes, labeled primer CINAU321F/CINAU321R can amplify about 1150bp product, and this can specifically follow the trail of haynaldia villosa 4VL chromosomes to primer.The compensatory translocation line of haynaldia villosa T4DS4VL homozygosis disclosed by the invention, can provide germ plasm resource for the excavation of haynaldia villosa 4VL chromosome favorable genes；The specific molecular marker of haynaldia villosa 4VL chromosomes can be specifically followed the trail of, available for molecular marker assisted selection breeding.
Description
Technical field
The invention belongs to biological technical field, be related to a special codominant marker of haynaldia villosa 4VL chromosomes and
Its primer and purposes.
Background technology
Cultivar hereditary basis is increasingly narrow to have turned into the main bottleneck (Li Zhen that wheat breeding makes a breakthrough
Sound, 2010).Wheat relative category contains abundant antibiont and the genetic resources such as abiotic stress and high yield, FOM,
Common wheat is imported by the excellent genes in distant hybridization nearly edge species, is to widen wheat genetic basis, promote wheat to educate
Kind make a breakthrough effective way (it is lucky perfectly sound etc., 2001；Zhang Zengyan etc., 2004；Wang Shumin etc., 2011).
Haynaldia villosa (Haynaldia villosa, genome VV) originates in Mediterranean, is the diploid of cultivated wheat
Nearly edge species, biological strain performance nearly all to powdery mildew is high anti-or immune, high anti-leaf rust, bar rust and stem rust, to total eclipse
Disease, eye spot, yellow mosaic also have preferable resistance, and also with winter resistance is good, drought-enduring, tillering ability is strong, spikelet number is more
With a variety of merits such as grain protein content height, be Genetic Characters In Wheat improvement important gene resource (De Pace C,
2011).Studies have found that on haynaldia villosa 4VL chromosomes have anti-wheat eye spot, control wheat coding alcohol soluble protein and
The gene of salt tolerance of wheat.In order to which using the favorable genes of haynaldia villosa 4VL chromosomes, this laboratory is currently same using part
Source pairing control system creates the different structure variation for being related to 4VL chromosomes of more clip sizes for carrying target gene
Body.The errorless identification exogenous chromosome identity of precise and high efficiency, to accelerating Wheat Germplasm Resources innovation and utilizing, improves external source dyeing
The efficiency of selection of body, reduction labour cost has important practical significance.Highdensity molecular labeling is for accurate identification external source
The transposition identity of chromosome is significant.But the specific molecular marker negligible amounts of each chromosome of current haynaldia villosa, and
And it is mainly distributed on 4VS and the 6VS dye that haynaldia villosa contains anti yellow flower leaf disease (WYMV) Wss1 genes and mildew-resistance Pm21 genes
On colour solid arm, and be distributed on other chromosomes it is less, thus limit favorable genes on other chromosomes excavation, positioning and profit
With.
EST (EST) is that into cDNA and mRNA reverse transcriptions are cloned into vector construction into after cDNA library, greatly
Scale selects cDNA clone at random, the short cDNA partial sequences that one-step method sequencing is obtained is carried out to itself 3' or 5' end, typically
Length is 300~500bp, represents a part for a complete genome.In addition to EST marks are the characteristics of with general molecular labeling,
Also have contain much information, versatility is good, exploitation is simple, quick, expense is low, easily operated, stability is good and repeated high excellent
Point, is received significant attention.EST comes from transcriptional domain, and its conservative is higher, and the versatility ratio between race and kind is from non-
The mark of EST is more preferable.Est sequence according to each section on chromosome of wheat is positioned at develops molecular labeling, can
With the homologue or chromosome segment of the corresponding nearly edge species of fast track, or even the target gene near respective regions is entered
Row positioning.In recent years, with the implementation that the plant such as wheat EST plans, the wheat crops logged in every year in ncbi database
(including wheat, barley, rye, oat and aegilops tauschii etc.) EST number rapid growths, for opening for the molecular labeling based on EST
Hair and the sequence information resource enriched using providing.Therefore, dyed using existing est sequence information development haynaldia villosa 4VL
The specific molecular marker of body has master for the excavation of the precise Identification and target gene of haynaldia villosa 4VL chromosomal structural variation bodies
Want meaning.
Source of plant material used is as follows in this research：Haynaldia villosa (Haynaldia villosa) is planted from Britain Camb
Introduce in thing garden；Durum wheat (Triticum durum L., numbering " in draw 1286 ", genome AABB) by Scientia Agricultura Sinica
Institute introduces a fine variety group and introduced from the international corn improvement center of Mexico；Common Wheat Varieties China spring (T.aestivum cv.Chinese
Spring, CS), durum-h. villosa amphiploid (T.durum-H.villosa amphiploid, also known as hard cluster wheat, base
Because of a group AABBVV), a set of common wheat China spring-haynaldia villosa alien addition lines DA1V-DA7V, T4DL4VS homozygosis transposition
System, T5DL4VL homozygosis translocation line and T4DS4VL homozygosis translocation line are provided by Agricultural University Of Nanjing's cytogenetics.
The content of the invention
It is an object of the invention to the drawbacks described above for prior art, there is provided a species specific molecular labeling primer.
It is a further object of the present invention to provide the application of the molecular labeling primer.
The purpose of the present invention can be achieved through the following technical solutions：
The specific molecular marker of haynaldia villosa 4VL chromosomes, selected from mark CINAU321；Mark CINAU321 primer sequence
It is classified as：CINAU321F：SEQ ID NO.1, CINAU321R：SEQ ID NO.2, with mark CINAU321 primer from containing cluster
About 1150bp specific band can be amplified in the plant of dirty wheat 4VL chromosomes.
The specific molecular marker primer of haynaldia villosa 4VL chromosomes of the present invention, selected from CINAU321F/
CINAU321R, primer CINAU321F sequences are：SEQ ID NO.1, primer CINAU321R sequences are：SEQ ID NO.2.This
The primer that the mark of haynaldia villosa 4VL chromosomes can be specifically followed the trail of in invention is to be set using wheat EST (BE637255) sequence as template
Obtained from meter.
Application of the molecular labeling of the present invention in identification carries haynaldia villosa 4VL chromosomes.
Application of the molecular labeling of the present invention in molecular marker assisted selection breeding.
The method that haynaldia villosa 4VL chromosomes are followed the trail of using molecular labeling of the present invention, including：With material to be identified
DNA enters performing PCR as template, with the primer of the specific molecular marker CINAU321 described in claim 2 and expanded；PCR primer
Native polyacrylamide gel electrophoresis is carried out, the plant that can amplify 1150bp specific bands is containing haynaldia villosa 4VL dyeing
The plant of body.
The preferred PCR amplification system of described method is：The 1 μ L DNA profilings containing 20-100ngDNA, 1.0 μ L 10 ×
PCR buffer, 0.8 μ L MgCl2, 0.8 μ L dNTP, primer CINAU321F/CINAU321R each 0.2 μ L, 0.15 μ L Taq
DNA polymerase, 5.85 μ L ddH2O；PCR programs are：94 DEG C of pre-degenerations 3 minutes；94 DEG C are denatured 30 seconds, 55 DEG C of annealing 50
Second, 72 DEG C extend 1 point 10 seconds, 35 circulations；72 DEG C extend 10 minutes；10 DEG C of preservations.
The preferred PCR primer of described method is in acrylamide and methene the proportion of acylamide 39:1 non denatured polypropylene
It is separated by electrophoresis, then is detected with argentation on acrylamide gel, the plant that can amplify 1150bp specific bands is containing haynaldia villosa 4VL
The plant of chromosome.
Beneficial effect：
1st, the primer of the molecular labeling that can specifically follow the trail of haynaldia villosa 4VL chromosomes disclosed in the present invention
(CINAU321F/CINAU321R) it is to be designed based on wheat est sequence, for identifying in plant whether contain
Haynaldia villosa 4VL chromosomes, simple and easy to apply, convenient and swift, amplification is stable.
2nd, the product and haynaldia villosa 4VL chromosomes of labeled primer CINAU321F/CINAU321R amplifications are closely coupled, so
Enter performing PCR to labeled primer with this to expand, it is high using the accuracy of molecular marker assisted selection haynaldia villosa 4VL chromosomes.
3rd, labeled primer CINAU321F/CINAU321R is codominant marker, can both follow the trail of exogenous chromosome, while
Chromosome of wheat can be followed the trail of.Therefore, such phenotypic marker can not only detect the exogenous chromosome imported in Wheat Background, also may be used
To analyze the change of chromosome of wheat.
Brief description of the drawings
Fig. 1：The compensatory translocation line of wheat-haynaldia villosa T4VL4DS homozygosis.
(a):The compensatory translocation line chromosome sectioning DAPI dyeing of wheat-haynaldia villosa T4VL4DS homozygosis；(b):Wheat-
Haynaldia villosa T4VL4DS homozygosis translocation line tip of a root mitotic chromosome mid-terms GISH schemes, and haynaldia villosa total genomic dna is used
Fluorescein-12-dUTP is marked, and green is presented；(c):The wheat-haynaldia villosa T4VL4DS homozygosis translocation line tips of a root have
Silk division chromosome mid-term FISH figures, oligonucleotide probe (pAs1-1,4,6) and pSc119.2-1 are used in 5 '-end respectively
TAMRA (6-carboxytetramethylrhodamine) is modified, and danger signal is presented.
Fig. 2：Wheat EST (BE637255) sequences and primer sequence corresponding to labeled primer CINAU321F/CINAU321R
Column position
Fig. 3：With haynaldia villosa, wheat-haynaldia villosa amphidiploid, wheat-haynaldia villosa 1V-7V alien addition lines, 4V (4D)
Alien substitution, the compensatory translocation line of T4DL4VS homozygosis, the DNA of T5DL4VL homozygosis translocation line and common wheat China spring make
For template, enter performing PCR with the labeled primer CINAU321F/CINAU321R in the present invention respectively and expand, as a result show that mark draws
Thing CINAU321F/CINAU321R haynaldia villosa, wheat-haynaldia villosa amphidiploid, wheat-haynaldia villosa 4V alien addition lines,
About 1150bp specific band is amplified in T5DL4VL homozygosis translocation lines.Arrow show specific band.
Note：
M：Molecular weight marker DL2000；1：Haynaldia villosa；2：Hard cluster wheat；3：Durum wheat；4：China spring；5：T4DL·4VS
Homozygosis translocation line；6：T5DL4VL homozygosis translocation lines；7-13：Wheat-haynaldia villosa alien addition lines DA1V-DA7V；14：Lack
The body 4B of body 4A- tetra-；15：The body 4B of nullisomic 4D- tetra-.
Embodiment
Embodiment 1
1st, the design of the special molecular labeling primer for following the trail of haynaldia villosa 4VL chromosomes
In developing and screening the specific molecular marker research of haynaldia villosa 4VL chromosomes, molecular labeling CINAU321 can be special
Different tracking haynaldia villosa 4VL chromosomes (Fig. 3).This primer to mark is according to the est sequence on the homologous group of wheat Part IV
(BE637255) (see reference document Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD et
al(2004)A chromosome bin map of 16,000expressed sequence tag loci and
distribution of genes among the three genomes of polyploid wheat.Genetics
168:701-712), designed and screened into (http using online primer-design software Primer3V0.4.0://
frodo.wi.mit.edu/primer3/)。
The primer for marking CINAU321 is CINAU321F：5'-TGTTGATGACATTCTGCACT-3'(SEQ ID NO.1)
And CINAU321R：5'-GCTGACGTATTTGTCTTTCC-3'(SEQ ID NO.2)；(Fig. 2)
Molecular Detections of the labeled primer CINAU321F/CINAU321R in haynaldia villosa 4VL chromosome materials are related to
Carried out using labeled primer CINAU321F/CINAU321R in haynaldia villosa 4VL chromosomal structural variation bodies are related to
PCR augmentation detections.As a result show：It is different additional in parent's haynaldia villosa, wheat-haynaldia villosa amphidiploid, wheat-haynaldia villosa 4V disomes
System,
About 1150bp specific band is amplified in T5DL4VL homozygosis translocation lines, and is not containing haynaldia villosa 4VL dyes
The specific band (Fig. 3) is not amplified in the material of colour solid.
PCR reagent is constituted：The 1.0 μ L DNA profilings of the DNA containing 20-100ng, 1.0 μ L10 × PCR buffer, 0.8 μ
LMgCl2, 0.8 μ LdNTP, primer CINAU321F and CINAU321R each 0.2 μ L, 0.15 μ L Taq DNA polymerase,
5.85μL ddH2O。
PCR programs are：94 DEG C of pre-degenerations 3 minutes；94 DEG C are denatured 30 seconds, and 55 DEG C are annealed 50 seconds, and 72 DEG C extend 1 point 10 seconds,
35 circulations；72 DEG C extend 10 minutes；10 DEG C of preservations, PCR primer is 39 in acrylamide and methene the proportion of acylamide:1
Non-denaturing polyacrylamide gel on be separated by electrophoresis, then dyed with argentation.
<110>Agricultural University Of Nanjing
<120>The special codominant marker of one haynaldia villosa 4VL chromosome and its primer and purposes
<160> 2
<210> 1
<211> 20
<212> DNA
<213>Artificial sequence
<220>
<223>Primer CINAU321F
<400> 1
tgttgatgac attctgcact 20
<210> 2
<211> 20
<212> DNA
<213>Artificial sequence
<220>
<223>Primer CINAU321R
<400> 2
gctgacgtat ttgtctttcc 20
Sequence table
1
Claims (7)
1. the specific molecular marker CINAU321 of haynaldia villosa 4VL chromosomes, it is characterised in that described mark CINAU321's
Primer sequence is：CINAU321F：SEQ ID NO.1, CINAU321R：SEQ ID NO.2, with above-mentioned primer from containing haynaldia villosa
About 1150bp specific band can be amplified in the plant of 4VL chromosomes.
2. the specific molecular marker CINAU321 of the haynaldia villosa 4VL chromosomes described in claim 1 primer, it is characterised in that
Primer CINAU321F sequences are：SEQ ID NO.1, primer CINAU321R sequences are：SEQ ID NO.2.
3. the primer of the specific molecular marker CINAU321 described in claim 2 carries haynaldia villosa 4VL chromosomes in identification
In application.
4. the primer of the specific molecular marker CINAU321 described in claim 2 answering in molecular marker assisted selection breeding
With.
5. follow the trail of the side of haynaldia villosa 4VL chromosomes using the primer of the specific molecular marker CINAU321 described in claim 2
Method, including：Using the DNA of material to be identified as template, with drawing for the specific molecular marker CINAU321 described in claim 2
Thing enters performing PCR amplification；PCR primer carries out native polyacrylamide gel electrophoresis, can amplify the plant of 1150bp specific bands
Strain is the plant containing haynaldia villosa 4VL chromosomes.
6. method according to claim 5, it is characterised in that PCR amplification system is：The 1 μ L DNA containing 20-100ngDNA
Template, 1.0 μ 10 × PCR of L buffer, 0.8 μ L MgCl2, 0.8 μ L dNTP, primer CINAU321F/CINAU321R each 0.2
μ L, 0.15 μ L Taq DNA polymerase, 5.85 μ L ddH2O；PCR programs are：94 DEG C of pre-degenerations 3 minutes；94 DEG C of denaturation
30 seconds, 55 DEG C were annealed 50 seconds, and 72 DEG C extend 1 point 10 seconds, 35 circulations；72 DEG C extend 10 minutes；10 DEG C of preservations.
7. method according to claim 5, it is characterised in that PCR primer is in acrylamide and methene the proportion of acylamide
39:It is separated by electrophoresis, then is detected with argentation on 1 non-denaturing polyacrylamide gel, 1150bp specific bands can be amplified
Plant is the plant containing haynaldia villosa 4VL chromosomes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710473068.5A CN107326070A (en) | 2017-06-21 | 2017-06-21 | The special codominant marker of one haynaldia villosa 4VL chromosome and its primer and purposes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710473068.5A CN107326070A (en) | 2017-06-21 | 2017-06-21 | The special codominant marker of one haynaldia villosa 4VL chromosome and its primer and purposes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107326070A true CN107326070A (en) | 2017-11-07 |
Family
ID=60195493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710473068.5A Pending CN107326070A (en) | 2017-06-21 | 2017-06-21 | The special codominant marker of one haynaldia villosa 4VL chromosome and its primer and purposes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107326070A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109492706A (en) * | 2018-11-27 | 2019-03-19 | 微医云(杭州)控股有限公司 | A kind of chromosome classification prediction meanss based on Recognition with Recurrent Neural Network |
| CN113528695A (en) * | 2021-06-23 | 2021-10-22 | 淮阴师范学院 | Haynaldia villosa 2VL chromosome specific RFLP-STS molecular marker and application thereof |
-
2017
- 2017-06-21 CN CN201710473068.5A patent/CN107326070A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| ZHAO REN-HUI ET AL.: "Development of EST-PCR Markers for the Chromosome 4V of Haynaldia villosa and Their Application in Identification of 4V Chromosome Structural Aberrants", 《JOURNAL OF INTEGRATIVE AGRICULTURE》 * |
| 袁静娅: "普通小麦-加州野大麦双二倍体辐射回交后代的分子细胞遗传学分析", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 陈全战 等: "普通小麦-簇毛麦易位系T4VS·4VL-4AL的选育与鉴定", 《作物学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109492706A (en) * | 2018-11-27 | 2019-03-19 | 微医云(杭州)控股有限公司 | A kind of chromosome classification prediction meanss based on Recognition with Recurrent Neural Network |
| CN109492706B (en) * | 2018-11-27 | 2020-12-01 | 微医云(杭州)控股有限公司 | Chromosome classification prediction device based on recurrent neural network |
| CN113528695A (en) * | 2021-06-23 | 2021-10-22 | 淮阴师范学院 | Haynaldia villosa 2VL chromosome specific RFLP-STS molecular marker and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Luo et al. | Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers | |
| CN105385697B (en) | Sesame inflorescence finite basis is because of Sidt1 and its SNP marker | |
| Gupta et al. | Integration of EST-SSR markers of Medicago truncatula into intraspecific linkage map of lentil and identification of QTL conferring resistance to ascochyta blight at seedling and pod stages | |
| CN107201404B (en) | Molecular biological identification method for sex of asparagus hermaphrodite plants and application thereof | |
| CN105838712B (en) | The SSR molecular marker isolated with cucumber powdery mildew resistance main effect QTL | |
| CN108330163B (en) | Characteristic sequence, primer and identification method of apocarya variety Nacono and Sumner | |
| CN103740828B (en) | SNP (Single Nucleotide Polymorphism) molecular marking method for major QTL (Quantitative Trait Locus) in fruit stem length of pear fruit and application thereof | |
| CN107058529A (en) | A kind of method of seed selection stripe rust of wheat durable resistance material | |
| US20190380290A1 (en) | Methods and compositions for watermelon sex expression | |
| CN107326070A (en) | The special codominant marker of one haynaldia villosa 4VL chromosome and its primer and purposes | |
| CN105821153B (en) | The relevant molecular labeling of rape cracking resistance angle character main effect QTL and application | |
| CN107619875A (en) | A kind of insertion and deletion marker site, primer and application for being used to identify Watermelon Fruit shape | |
| CN107586881B (en) | Corn molecular marker and application thereof in detecting corn flowering phase related traits | |
| CN106480066B (en) | The new gene Wbph9 (t) and its molecule labelling method of a kind of Rice Resistance white backed planthopper and application | |
| KR20150121794A (en) | Primer set for selecting cucumber downy mildew disease resistant variety and selection method using the same | |
| CN108588261A (en) | A kind of identification is located at InDel primers and its application of the late bolting QTL on radish R02 chromosomes | |
| CN107746895A (en) | It is a kind of it is low-phosphorous under the conditions of lifted barley harvest index QTL site molecular labeling and application | |
| CN104762299B (en) | A kind of rice seedling resistant gene of salt qST2 and its molecule labelling method | |
| CN106995846A (en) | The detection and application of a kind of Henan south aromatic rice Badh2 gene functions mark | |
| CN105695589A (en) | Complete set of reagent and molecular marker for detecting containing of 6V#4S chromosome arm of dasypyrum villosum in wheat | |
| CN113278723A (en) | Composition for analyzing genetic diversity of Chinese cabbage genome segment or genetic diversity introduced in synthetic mustard and application | |
| CN116144820B (en) | Indel mark with cucumber root re-regulating and controlling gene linkage and application thereof | |
| CN105385772B (en) | Primer for screening wheat leaf rust resistance QTL and its application | |
| CN111004857A (en) | Molecular marker primer of major QTL (quantitative trait locus) site of soybean branch number and application of molecular marker primer | |
| CN110195125A (en) | The linkage molecule of Parthenocarpy in Cucumber main effect QTL Parth2.1 marks and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171107 |