CN107058166A - The Lactobacillus plantarum of one plant of extracellular polysaccharide - Google Patents
The Lactobacillus plantarum of one plant of extracellular polysaccharide Download PDFInfo
- Publication number
- CN107058166A CN107058166A CN201710025113.0A CN201710025113A CN107058166A CN 107058166 A CN107058166 A CN 107058166A CN 201710025113 A CN201710025113 A CN 201710025113A CN 107058166 A CN107058166 A CN 107058166A
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- CN
- China
- Prior art keywords
- adsorption
- lactobacillus plantarum
- polysaccharide
- exocellular polysaccharide
- lcc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 66
- 150000004676 glycans Chemical class 0.000 title claims abstract description 65
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 65
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 30
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 30
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 30
- 238000001179 sorption measurement Methods 0.000 claims abstract description 46
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 30
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229960000907 methylthioninium chloride Drugs 0.000 claims abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims abstract description 7
- 239000002105 nanoparticle Substances 0.000 claims abstract description 3
- 238000004321 preservation Methods 0.000 claims abstract description 3
- 239000000975 dye Substances 0.000 claims description 18
- 229920006395 saturated elastomer Polymers 0.000 claims description 13
- 239000010865 sewage Substances 0.000 claims description 4
- 238000002845 discoloration Methods 0.000 abstract description 4
- 238000001338 self-assembly Methods 0.000 abstract description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 34
- 239000000243 solution Substances 0.000 description 23
- 241000894006 Bacteria Species 0.000 description 20
- 239000000523 sample Substances 0.000 description 18
- 235000014655 lactic acid Nutrition 0.000 description 17
- 239000004310 lactic acid Substances 0.000 description 17
- 238000010521 absorption reaction Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 12
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 10
- 229920002444 Exopolysaccharide Polymers 0.000 description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
- 150000002500 ions Chemical class 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 239000008186 active pharmaceutical agent Substances 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- XIEPJMXMMWZAAV-UHFFFAOYSA-N cadmium nitrate Inorganic materials [Cd+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XIEPJMXMMWZAAV-UHFFFAOYSA-N 0.000 description 4
- XTVVROIMIGLXTD-UHFFFAOYSA-N copper(II) nitrate Chemical compound [Cu+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XTVVROIMIGLXTD-UHFFFAOYSA-N 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- MCPLVIGCWWTHFH-UHFFFAOYSA-L methyl blue Chemical compound [Na+].[Na+].C1=CC(S(=O)(=O)[O-])=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[NH+]C=2C=CC(=CC=2)S([O-])(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S([O-])(=O)=O)=CC=2)C=C1 MCPLVIGCWWTHFH-UHFFFAOYSA-L 0.000 description 4
- NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 4
- 241000192125 Firmicutes Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 229920001503 Glucan Polymers 0.000 description 2
- 229920000869 Homopolysaccharide Polymers 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002389 environmental scanning electron microscopy Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- 230000006920 protein precipitation Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 238000005491 wire drawing Methods 0.000 description 2
- ZGXJTSGNIOSYLO-UHFFFAOYSA-N 88755TAZ87 Chemical compound NCC(=O)CCC(O)=O ZGXJTSGNIOSYLO-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 229910052695 Americium Inorganic materials 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000589776 Pseudomonas putida Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 229910052776 Thorium Inorganic materials 0.000 description 1
- 229910052770 Uranium Inorganic materials 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229910052768 actinide Inorganic materials 0.000 description 1
- 150000001255 actinides Chemical class 0.000 description 1
- VBIXEXWLHSRNKB-UHFFFAOYSA-N ammonium oxalate Chemical compound [NH4+].[NH4+].[O-]C(=O)C([O-])=O VBIXEXWLHSRNKB-UHFFFAOYSA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000021001 fermented dairy product Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009854 hydrometallurgy Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 229940118199 levulan Drugs 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000013528 metallic particle Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- -1 mining industry Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000006872 mrs medium Substances 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000006385 ozonation reaction Methods 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000010970 precious metal Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 229910052705 radium Inorganic materials 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000021108 sauerkraut Nutrition 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/285—Treatment of water, waste water, or sewage by sorption using synthetic organic sorbents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/308—Dyes; Colorants; Fluorescent agents
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/40—Organic compounds containing sulfur
Abstract
The invention discloses the Lactobacillus plantarum of one plant of extracellular polysaccharide, its Classification And Nomenclature is Lactobacillus plantarum, and bacterial strain number is LCC 605, has been preserved in China typical culture collection center, deposit number is CCTCC M 2016491, and preservation date is September 18 in 2016.Compared with existing bacterial strain, the extracellular polysaccharide of the institutes of Lactobacillus plantarum LCC 605 of the invention finds that nano particle can be self-assembly of first, and Adsorption of Heavy Metals and methylene blue ability are most strong, to methylene blue, Pb2+、Cd2+And Cu2+Adsorbance be respectively 3029mg/g, 1513mg/g, 2097mg/g and 2987mg/g, available for biological prosthetic heavy metal and dye discoloration with Environment control, with environment friendly and sustainable developability.
Description
Technical field
The invention belongs to biotechnology and field of food fermentation, and in particular to the Lactobacillus plantarum of one plant of extracellular polysaccharide.
Background technology
Exopolysaccharides Produced by Lactic Acid Bacteria is the macromolecule polysaccharide polymer produced by lactic acid bacteria, can be divided into capsular polysaccharide and extracellular many
Sugar.Capsular polysaccharide is typically distributed across on the cell membrane of thalline, and exocellular polysaccharide is generally released in nutrient solution.Lactic acid bacteria is extracellular
Polysaccharide has fabulous application (FEMS in terms of rheological properties, texture and the palatability of fermented dairy product is improved
microbiology reviews,1999,23(2):153-177).Exopolysaccharides Produced by Lactic Acid Bacteria can for a long time exist in enteron aisle
And then increase field planting (International dairy journal, 2002,12 (2) of the probiotics in enteron aisle:163-171).
In addition, it has been found that Exopolysaccharides Produced by Lactic Acid Bacteria heavy metal ion and dye molecule also have certain adsorption effect
(Bioresource technology,2014,160:15-23；Plos one,2016,11(2):e0148430).Lactic acid mycetocyte
Exo polysaccharides can be divided into homopolysaccharide and different polysaccharide according to its Nomenclature Composition and Structure of Complexes.Homopolysaccharide mainly has glucan and levulan.Compare
Under, different polysaccharide is then made up of the oligosaccharide of multiple repetitions, and each repeat unit usually contains two or more monose simultaneously
With different connected mode (Biotechnology advances, 2001,19 (8):597-625).Exopolysaccharides Produced by Lactic Acid Bacteria
With some charged groups, such as carboxyl, phosphate radical and hydroxyl.These groups contribute to exocellular polysaccharide Adsorption of Heavy Metals.
Many metal ions or metallic particles, including poisonous heavy metal ion such as Pb, Cd, Cr are there is in natural environment
Deng, some yttriums such as Au, Ag, Pd, Pt etc., and some actinides such as U, Th, Ra, Am etc..Environment and food
The phenomenon of heavy metal accumulation has great threat to human health in organism caused by the heavy metal pollution of product.Meanwhile, weight
Metallic pollution toxicity is larger and is widely present.In addition to the natural activity of the mankind, other human production activities can also produce
Heavy metal ion such as mining industry, refining industry, industry processes, food processing process, personal care product, industrial discharge,
Medicine company and cosmetics discarded object.In addition to heavy metal pollution, dye discoloration also can not be ignored.The color that the discharge of dyestuff is brought
Not only make us not pleasing, but also light can be hindered to permeate, endanger the growth of aquatic organism.Meanwhile, most of dyestuff is all poisonous
It is even carcinogenic, there is larger toxic action to organism.Traditional heavy metal, dyestuff minimizing technology include cohesion and
Flocculation, oxidation and ozonisation, membrane separation technique and biosorption technology.It is difficult processing by the waste water of dye discoloration, because
Belong to diehard for dye molecule, have certain repellence to aerobic digestion；In addition, dye molecule comparision contents in waste water
It is low, it is not easy to carry out the processing of extensive low cost.Discovered in recent years biosorption process can solve these problems, and into
This is relatively low, it is verified that effective and feasible (Biotechnology advances, 2008,26 (3):266-291).Also someone uses
Biosorption process reclaims Precious Metals Resources (Hydrometallurgy 103,180-189).Therefore, environmental protection is selected, can be held
The sorbing material heavy metal of supervention exhibition and dye discoloration progress processing are very important.
Exocellular polysaccharide is used for Adsorption of Heavy Metals it has been reported that as from Bacillus subtilis and Pseudomonas
Putida EPS can dramatically increase Cu2+Absorption (Bioresource technology, 2011,102 (2):1137-1141；
International biodeterioration&Biodegradation,2010,64(8):734-741)。Bacillus
licheniformis(Environmental pollution,2011,159(5):EPS heavy metals absorption 1369-1374)
Selectivity also have been reported that.Arthrobacter ps-5 exocellular polysaccharides can be with Adsorption of Cu2+、Pb2+And Cd2+(Carbohydrate
polymers,2014,101:50-56).In a word, the adsorption capacity of EPS heavy metals and EPS species, the kind of heavy metal ion
Class and environmental condition have much relations.But existing most of sorbing materials or without preferable adsorption effect, or
Person is to need the long period to adsorb.Accordingly, it would be desirable to develop that a kind of adsorption time is shorter and adsorption effect preferably have can
The biological adsorption agent of sustainable development, the improvement for heavy metal and dyestuff.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of Lactobacillus plantarum of extracellular polysaccharide, to solve prior art
Not the problems such as adsorption effect of presence is not good and adsorption time is longer.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
The Lactobacillus plantarum of one plant of extracellular polysaccharide, its Classification And Nomenclature is Lactobacillus plantarum, bacterial strain number
For LCC-605, China typical culture collection center has been preserved in, address is Wuhan University of Wuhan, China city, and postcode is
430072, deposit number is CCTCC M 2016491, and preservation date is September 18 in 2016.
The Lactobacillus plantarum LCC-605 of above-mentioned extracellular polysaccharide is that on January 15th, 2015 is sour from Yunnan Province Fuyuan County natural resources
Screening is obtained in dish.
The lactic acid bacteria LCC-605 of above-mentioned extracellular polysaccharide is gram-positive bacteria, catalase reaction negative；It is accredited as through API
Lactobacillus plantarum；Through molecular biology identification, it is nearest with Lactobacillus plantarum affiliation；Apply for gene accession number on NCBI
For KX443590 (undisclosed).
A kind of exocellular polysaccharide, it is produced as the Lactobacillus plantarum LCC-605 described in claim 1, is named as
EPS-605。
Wherein, it can be self-assembled into 50~300nm of diameter spherical nanoparticle.
Applications of the above-mentioned exocellular polysaccharide EPS-605 in Adsorption of Heavy Metals and dyestuff is also within the scope of the present invention.
Wherein, described heavy metal is Pb2+、Cd2+And Cu2+。
Wherein, described dyestuff is methylene blue.
Wherein, described heavy metal is the heavy metal in sewage.
Wherein, described dyestuff is the dyestuff in sewage.
Wherein, adsorption temp is 15~40 DEG C；
Wherein,
In Pb2+Initial concentration when being 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is 1513mg/g,
Saturated adsorption time is 6~8h；
In Cd2+Initial concentration when being 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is 2097mg/g,
Saturated adsorption time is 18~24h；
In Cu2+Initial concentration when being 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is 2987mg/g,
Saturated adsorption time is 8~12h；
When the initial concentration of methylene blue is 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is
3029mg/g, saturated adsorption time is 18~24h.
Above-mentioned application process comprises the following steps：
(1) prepared by Lactobacillus plantarum LCC-605 and purify exocellular polysaccharide EPS-605；
(2) the exocellular polysaccharide EPS-605 absorption dyestuffs prepared in step (1) are utilized.
Wherein, it can adopt and prepare and purify exocellular polysaccharide by Lactobacillus plantarum with the following method：
Lactobacillus plantarum LCC-605 thalline sample is taken, is activated three times with MRS culture mediums.4 DEG C, 14000g centrifugations
30min, to remove thalline.Supernatant liquor is poured into beaker after centrifugation, remaining thalline is centrifuged once again, and supernatant liquor is again
Pour into beaker.Then poured into beaker 80% trichloroacetic acid, until trichloroacetic acid final concentration of 4%, be put into 4 DEG C of refrigerator mistakes
Night, protein precipitation.Every other day, the solution in beaker is centrifuged again, with the protein of precipitation and separation.Precipitation is removed, upper strata is poured out
Clear liquid, adds the absolute ethyl alcohol of three times volume, 4 DEG C of refrigerator overnights is put into, to precipitate exocellular polysaccharide.Every other day, by beaker
Solution centrifugal, precipitation is polysaccharide.Polysaccharide is taken out and adds a small amount of ultra-pure water to dissolve, is fitted into bag filter, being put into large beaker makes
It is dialysed three days, a water is changed per 4h, to remove monose therein.After three days, it is placed in vacuum freeze drier and freezes, produce
Lactobacillus plantarum LCC-605 exocellular polysaccharide EPS-605.
Beneficial effect：
Compared with prior art, the present invention has following advantage：
1st, the new Lactobacillus plantarum LCC-605 identified that separates has biological safety, does not produce toxin.
2nd, exocellular polysaccharide EPS-605 Adsorption of Heavy Metals and methylene blue ability are strong, are highest in domestic and foreign literature report.
It is to methylene blue, Pb2+、Cd2+And Cu2+Adsorbance be respectively 3029mg/g, 1513mg/g, 2097mg/g and 2987mg/
g。
3rd, exocellular polysaccharide ferments from Lactobacillus plantarum, therefore with environment friendly and sustainable developability.
4th, find that non-glucan exocellular polysaccharide EPS-605 can be self-assembly of 80~300nm nanometer spherical structure first.
Brief description of the drawings
Figure 1A is lactic acid bacteria plate screening schematic diagram in embodiment 1；
Figure 1B is the fermentation broth viscosity schematic diagram of production polysaccharide-forming lactic acid bacteria in embodiment 1；
Fig. 1 C are wire drawing experimental result schematic diagram in embodiment 1；
Fig. 1 D are Gram's staining result schematic diagram in embodiment 2；
The scanning electron microscope (SEM) photograph that Fig. 2 is Lactobacillus plantarum LCC-605 in embodiment 2；
Fig. 3 is the exocellular polysaccharide produced under the conditions of different carbon source in embodiment 3 to Pb2+、Cd2+、Cu2+Absorption comparison diagram；
Fig. 4 be in embodiment 3 the different adsorption times of the exocellular polysaccharide that produces to Pb2+、Cd2+、Cu2+Absorption comparison diagram；
Fig. 5 is the exocellular polysaccharide produced under the conditions of different carbon source in embodiment 3 to Pb2+ESEM contrast after absorption
Figure；
Fig. 6 is the result of exocellular polysaccharide is shown before exocellular polysaccharide absorption methylene blue in embodiment 4 and after absorption methyl blue
It is intended to；
Fig. 7 adsorbs the schematic diagram that methyl blue is changed over time for exocellular polysaccharide in embodiment 4.
Fig. 8 is the Mb residual quantity change schematic diagrams in solution in embodiment 4.
Embodiment
Embodiment 1
Lactobacillus plantarum LCC-605 separation screening, comprises the following steps：
(1) fermented sample liquid sample is derived from the local Homemade sauerkraut zymotic fluid in Qujing.
(2) dilution of sample liquid：With sterile 1mL pipette, extract 1mL sample liquids nothing is used in sterile small test tube
Bacterium normal saline dilution into dilution factor be 10-1、10-2、10-3、10-4、10-5、10-6、10-7、10-8、10-9、10-10、10-11、10-12.It is 10 to select dilution factor-4To 10-12It is standby.
(3) flat board is separated：First on super-clean bench under conditions of sterile working, by sterilized containing calcium carbonate
MRS solid mediums are down flat plate, and it is 10 that above dilution factor is drawn after temperature is room temperature-4To 10-12Zymotic fluid 0.1mL.Respectively
On the culture dish for pouring into and being applied to the MRS solid mediums containing calcium carbonate with tilt-pour process and rubbing method, each dilution factor is done
Two repetitions, and installed with preservative film in the insulating box as 37 DEG C and cultivate 48h.As shown in figure 1, filtering out the bacterium of transparent circle
Fall, and carry out Liquid Culture, obtain the Lactobacillus plantarum LCC-605 of extracellular polysaccharide thalline sample (Figure 1A).
(4) wire drawing experiment determination production polysaccharide, as illustrated in figures ib and 1 c, bacterial strain production are carried out to the Lactobacillus plantarum of acquisition
Raw viscous polysaccharide.
The identification of the bacterial strain of embodiment 2 and the preparation of exocellular polysaccharide
(1) thalli morphology observation and Gram's staining
The thalline sample for choosing the collocation prepared in a small amount of embodiment 1 with oese in super-clean bench is uniformly applied to load glass
On piece, then with alcolhol burner heating kill bacterium, ammonium oxalate crystal violet is added dropwise, 1min is dyed.Washed out with water and wash unnecessary on slide
Dyeing liquor, then blotted with blotting paper.A small amount of Wagner's reagent is added dropwise, stands and is washed after 1min, blotted with blotting paper, even
The continuous ethanol that is added dropwise is decolourized, until the liquid colorless of outflow, is then washed.Finally 30s is redyed with Huang red dye liquor.Dyeing terminates
Afterwards, observed with microscope, gram-positive bacteria is in purple, Gram-negative bacteria takes on a red color.Qualification result shows separation
Lactic acid bacteria is gram-positive bacteria (Fig. 1 D).
(2) API is tested
The Lactobacillus plantarum LCC-605 of the extracellular polysaccharide obtained in Example 1 thalline sample, in MRS culture dishes
Middle line, is put into 37 DEG C of 24~48h of incubator culture.The picking individual colonies from culture dish, are inserted in API 50CHL culture mediums,
Indentifying substance bar is accessed, 24~48h is further cultured in 37 DEG C of incubators, record sample bacterial strain is in 24h and 48h for carbon aquation
The result of compound fermentation, the data obtained input API LAB PLUS softwares are identified.API testing results are shown in Table 1.LCC-
605 can utilize several kinds of carbon source, such as glucose, galactolipin, xylose, antierythrite, sucrose, rhamnose.
The API experimental results of table 1
(2) prepared by exocellular polysaccharide
The Lactobacillus plantarum LCC-605 of the extracellular polysaccharide obtained in Example 1 thalline sample, is lived with MRS nutrient solutions
Change three times, 4 DEG C, 14000g centrifugation 30min, to remove thalline.Supernatant liquor is poured into beaker after centrifugation, remaining thalline is again
Once, supernatant liquor pours into beaker again for centrifugation.Then poured into beaker 80% trichloroacetic acid, until trichloroacetic acid is dense eventually
Spend for 4%, be put into 4 DEG C of refrigerator overnights, protein precipitation.Every other day, the solution in beaker is centrifuged again, with the albumen of precipitation and separation
Matter.Precipitation is removed, supernatant liquor is poured out, adds the absolute ethyl alcohol of three times volume, be put into 4 DEG C of refrigerator overnights, it is extracellular to precipitate
Polysaccharide.Every other day, by solution centrifugal, polysaccharide precipitation.Polysaccharide is taken out and adds a small amount of ultra-pure water to dissolve, is fitted into bag filter, is put into
Large beaker makes it dialyse three days, a water is changed per 4h, to remove monose therein.After three days, it is placed in vacuum freeze drier
It is lyophilized, produce the Lactobacillus plantarum LCC-605 of extracellular polysaccharide exocellular polysaccharide.
(3) LCC-605 ESEMs result can be seen that lactic acid bacteria for rod-short and secret out of exocellular polysaccharide spherical in shape
Particle (Fig. 2).
The detection of the heavy metal adsorption of embodiment 3：
(1) influence of the different carbon source to biological adsorption effect
In order to probe into the carbon source for causing Exopolysaccharides Produced by Lactic Acid Bacteria to produce best biological adsorption effect, using containing different carbon
Source MRS culture mediums are fermented.With not carbonaceous sources and respectively containing glucose, mannose, sucrose, lactose MRS medium cultures
After lactic acid bacteria, fermentation, extraction exocellular polysaccharide, this six kinds of polysaccharide are each configured to 0.8mg/mL solution, are divided into 18 parts, loading
In bag filter.10mg/L plumbi nitras, cadmium nitrate and each 6 parts of copper nitrate solution is configured, by the extracellular many of 6 different carbon source cultures
Sugared bag filter is respectively charged into 3 kinds of solions, is stood and is carried out concentration of metal ions detection to solion sampling after 24h.It is real
Test result and see Fig. 3, the exocellular polysaccharide produced by carbon source of glucose is to Pb2+Adsorption capacity it is most strong.
(2) influence that adsorption time is acted on biological adsorption
10mg/L plumbi nitras, cadmium nitrate, copper nitrate solution is prepared, is poured into 9 beakers.By lactic acid bacterium bag exo polysaccharides sample
Product are made into 0.8mg/mL solution with deionized water, are fitted into 9 bag filters, and being respectively put into 9 beakers makes it start biological suction
Attached effect.The sample for respectively taking a heavy metal ion solution every 0,4,8,12,24,28,48h carries out Concentration Testing, as a result such as
Shown in Fig. 4, to Cd2+And Cu2+Be adsorbed onto 12h nearly reach stabilization, to Pb2+Absorption 24h nearly reach stabilization.
(3) influence that pH is acted on biological adsorption
10mg/L plumbi nitras, cadmium nitrate, copper nitrate solution is prepared, pours into 10 beakers, pH is tuned into 3,5,7,9 and
11.Every group two parallel.Exopolysaccharides Produced by Lactic Acid Bacteria sample is made into 0.8mg/mL solution with deionized water, loads 10 dialysis
In bag, being respectively put into 10 beakers makes it start biological adsorption effect.The sample of heavy metal ion solution is taken to carry out after absorption 24h
Concentration Testing.As a result such as Fig. 5, Pb2+The adsorbance highest in the range of pH 5-11.
(4) influence that temperature is acted on biological adsorption
Prepare 10mg/L plumbi nitras, cadmium nitrate, copper nitrate solution.Lactic acid bacterium bag exo polysaccharides sample is matched somebody with somebody with deionized water
Into 0.8mg/mL solution, be fitted into bag filter, be respectively put into beaker respectively 25,28,31,34 and 37 DEG C carry out biological adsorption
Effect.The sample of heavy metal ion solution is taken to carry out Concentration Testing after 24h.As a result as shown in fig. 6, temperature heavy metal ion
Adsorption Effect is little.
The methylene blue adsorption number ability of embodiment 4 is detected.
Methylene blue is made into 20mg/L solution, takes 50mL to be placed in small beaker；The born of the same parents that will be prepared in embodiment 2
Exo polysaccharides are made into 0.8mg/mL, take 5mL to be put in 7KDa bag filter, put it into the small beaker containing methyl blue solution
In 25 DEG C of static 24h, a sample is taken every 2h, remaining methyl blue content in solution is determined, experimental result is shown in Fig. 7 and 8, EPS
The color of Methylene Blue in Solution substantially weakens after absorption, and absorption nearly reaches stabilization in 12h.
Claims (9)
1. the Lactobacillus plantarum of one plant of extracellular polysaccharide, its Classification And Nomenclature is Lactobacillus plantarum, bacterial strain number is
LCC-605, has been preserved in China typical culture collection center, and deposit number is CCTCC M 2016491, and preservation date is
September 18 in 2016.
2. a kind of exocellular polysaccharide, it is characterised in that it is produced as the Lactobacillus plantarum LCC-605 described in claim 1, life
Entitled EPS-605.
3. exocellular polysaccharide according to claim 2, it is characterised in that it can be self-assembled into the spherical of 50~300nm of diameter
Nano particle.
4. the exocellular polysaccharide EPS-605 that the Lactobacillus plantarum LCC-605 described in claim 2 is produced is in Adsorption of Heavy Metals and dyestuff
In application.
5. application according to claim 4, it is characterised in that described heavy metal is Pb2+、Cd2+And Cu2+。
6. application according to claim 4, it is characterised in that described dyestuff is methylene blue.
7. application according to claim 4, it is characterised in that described heavy metal is the heavy metal in sewage.
8. application according to claim 4, it is characterised in that described dyestuff is the dyestuff in sewage.
9. the application according to claim 5 or 6, it is characterised in that adsorption temp is 15~40 DEG C；
Wherein,
In Pb2+Initial concentration when being 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is 1513mg/g, saturation
Adsorption time is 6~8h；
In Cd2+Initial concentration when being 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is 2097mg/g, saturation
Adsorption time is 18~24h；
In Cu2+Initial concentration when being 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is 2987mg/g, saturation
Adsorption time is 8~12h；
When the initial concentration of methylene blue is 0~1000mg/L, exocellular polysaccharide EPS-605 saturated extent of adsorption is 3029mg/
G, saturated adsorption time is 18~24h.
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