CN110089666A - A kind of lactobacillus plantarum and its exocellular polysaccharide - Google Patents
A kind of lactobacillus plantarum and its exocellular polysaccharide Download PDFInfo
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- CN110089666A CN110089666A CN201910406712.6A CN201910406712A CN110089666A CN 110089666 A CN110089666 A CN 110089666A CN 201910406712 A CN201910406712 A CN 201910406712A CN 110089666 A CN110089666 A CN 110089666A
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- lactobacillus plantarum
- heavy metal
- exocellular polysaccharide
- lactobacillus
- eps
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- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 50
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 50
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 48
- 150000004676 glycans Chemical class 0.000 title claims abstract description 16
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 16
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 16
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 34
- 238000001179 sorption measurement Methods 0.000 claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 238000009629 microbiological culture Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 11
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000000729 antidote Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims description 2
- 229920002684 Sepharose Polymers 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 238000000502 dialysis Methods 0.000 claims description 2
- 229940097043 glucuronic acid Drugs 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 238000003809 water extraction Methods 0.000 claims description 2
- 241000186660 Lactobacillus Species 0.000 claims 2
- 229940039696 lactobacillus Drugs 0.000 claims 2
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 claims 1
- 239000012043 crude product Substances 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 10
- 238000009825 accumulation Methods 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000000274 adsorptive effect Effects 0.000 abstract description 2
- 238000013329 compounding Methods 0.000 abstract description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 16
- 241000894006 Bacteria Species 0.000 description 10
- 239000004310 lactic acid Substances 0.000 description 8
- 235000014655 lactic acid Nutrition 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 150000002500 ions Chemical class 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 229920002271 DEAE-Sepharose Polymers 0.000 description 2
- 229920002444 Exopolysaccharide Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
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- 238000013459 approach Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
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- 210000002249 digestive system Anatomy 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
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- 235000013601 eggs Nutrition 0.000 description 1
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- 210000000720 eyelash Anatomy 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
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- 230000006870 function Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 235000021474 generally recognized As safe (food) Nutrition 0.000 description 1
- 235000021473 generally recognized as safe (food ingredients) Nutrition 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 231100000003 human carcinogen Toxicity 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- -1 saccharide compound Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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- Organic Chemistry (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
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- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
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- Polymers & Plastics (AREA)
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Abstract
The invention discloses a kind of lactobacillus plantarum and its exocellular polysaccharides, strain classification is named as Lactobacillus plantarum, bacterial strain number is Lactobacillus plantarum 18-2, it has been preserved in China General Microbiological culture presevation administrative center, deposit number is CGMCC No.17566, and the deposit date is on April 12nd, 2019.Lactobacillus plantarum and its exocellular polysaccharide provided by the invention, it is obvious to the adsorption effect of heavy metal lead, on the one hand, the Lactobacillus plantarum 18-2 bacterial strain with heavy metal adsorption effect can be used as leavening applied in food production, prevent the accumulation of heavy metal lead；On the other hand, the produced EPS of Lactobacillus plantarum 18-2 bacterial strain has the function of excellent absorption heavy metal lead, can be used as heavy metal lead Adsorptive ansidoie individually or compounding is added, prevent the pollution and accumulation of heavy metal lead, ensure food safety.
Description
Technical field
The present invention relates to microorganisms technical field more particularly to a kind of lactobacillus plantarums and its exocellular polysaccharide.
Background technique
With the propulsion of process of industrialization, heavy metal pollution is increasingly severe, and heavy metal pollution not only causes greatly
Economic loss, and jeopardize human health, lead (Pb) is announced strongest to human toxicity as FAO (Food and Agriculture Organization of the United Nation) (FAO)
One of heavy metal, it is widely distributed in the environment, and be easy to be enriched with by bio-absorbable, then pass through the accumulation amplification mechanism of food chain
The mankind are acted on, and then endanger human health.
Cannot effectively degrade heavy metal and heavy metal of microorganism in environment can not be decomposed by chemical reaction, therefore a huge sum of money
The toxicity of category is not directly eliminated, can only by change heavy metal existence form or remove it from food or human body come
Mitigate the toxic action of heavy metal.At this stage mitigate heavy metal pollution harm main method have physical method, chemical method with
And biological method.Physical means mainly have membrane separation process and active carbon adsorption etc., and chemical means mainly have the precipitation method and electrolysis
Method etc..Compared to traditional physics and chemical method, microbial adsorbent is abundant with strain, cost is relatively low, without secondary pollution
The advantages that.
In the ecosystem, the main path of heavy metal pollution is water and food.Aquatic animal, such as fish are animal eggs
White effective source also thus becomes one of the main source of heavy metal in human body pollution.Heavy metal in food processing and production
Pollution source mainly have contaminated food supplementary material, food additives, packaging material for food and food processing, storage and
The heavy metal pollution etc. of transport.Lead is considered as human carcinogen, and respiratory system and digestive system are the weights of human body lead contamination
Approach is wanted, the mankind are exposed in the lead ring border of certain level possible injuring nerve system, hemopoietic system and cardiovascular system, even
Lead to the decline of memory and intelligence.In face of such severe health problem and food-safety problem, traditional control method effect
It is poor and unsuitable applied in food industry.Therefore, the safely and effectively food-grade control agent of research absorption heavy metal has been compeled
In the eyebrows and eyelashes.
Lactic acid bacteria is generally existing in the gastrointestinal tract of animal and people, food and environment, not pathogenic, some lactic acid bacterias are reasonable
The medical care effect thought and important physiological function, it is considered to be safe (Generally regarded as safe,
GRAS) food-grade microorganisms.It is reported that lactic acid bacteria has certain suction-operated to lead (Pb), and it can protect host's intestines
Road barrier.Exopolysaccharides Produced by Lactic Acid Bacteria (EPS) is that lactic acid bacteria is secreted into outside cell wall during growth metabolism, seeps in culture medium
A kind of saccharide compound.Viscosity, stability, emulsibility and the guarantor of food can be increased when lactic acid bacteria EPS is added in food
It is aqueous, while the mouthfeel, quality and flavor of food can be improved.It is reported that EPS has antitumor, anti-oxidant, the immune function of adjusting
The effects significant to human health such as energy.However existing Exopolysaccharides Produced by Lactic Acid Bacteria secretory volume is extremely limited, leads to cream
The adsorption effect of sour bacterium heavy metal be not very well, it is good, sustainable development therefore, it is necessary to develop the adsorption effect of a heavy metal species
Biological adsorption agent.
Summary of the invention
The present invention in view of the above technical problems, provides a kind of lactobacillus plantarum and its exocellular polysaccharide.On the one hand, there is a huge sum of money
The Lactobacillus plantarum 18-2 bacterial strain (CGMCC No.17566) for belonging to suction-operated can be used as leavening application
In food production, the accumulation of heavy metal lead is prevented；On the other hand, Lactobacillus plantarum 18-2 bacterial strain is produced
EPS has the function of excellent absorption heavy metal lead, can be used as heavy metal lead Adsorptive ansidoie individually or compounding is added, prevent weight
The pollution and accumulation of metallic lead, ensure food safety.
To achieve the goals above, the invention provides the following technical scheme:
A kind of lactobacillus plantarum provided by the invention, classification naming are Lactobacillus plantarum, bacterial strain number
For Lactobacillus plantarum 18-2, it is preserved in China General Microbiological culture presevation administrative center, preservation is compiled
Number be CGMCC No.17078, the deposit date is on April 23rd, 2019.
Lactobacillus plantarum 18-2 of the present invention is the nothing from the traditional dairy products dried milk cake of the Inner Mongol
Bacterium sampling, is used using coubling dilution and contains 0.5%CaCO3MRS agar medium separation, then with MRS agar medium scribing line
Acquisition is further purified in culture.
The gram stain microscopy result of Lactobacillus plantarum 18-2 is the positive (as shown in Figure 1, cell
It is dyed to purple).
Lactobacillus plantarum 18-2 cytomorphology is as follows: with physicochemical property
Through being sequenced, the 16s rDNA sequence of Lactobacillus plantarum 18-2 is different from other bacterial strains, sequence
Column are as shown in SEQ ID No.1.
SEQ ID No.1
ATACATGCAGTCGAACGAACTCTGGTATTGATTGGTGCTTGCATCATGATTTACATTTGAGTGAGTGGC
GAACTGGTGAGTAACACGTGGGAAACCTGCCCAGAAGCGGGGGATAACACCTGGAAACAGATGCTAATACCGCATAA
CAACTTGGACCGCATGGTCCGAGTTTGAAAGATGGCTTCGGCTATCACTTTTGGATGGTCCCGCGGCGTATTAGCTA
GATGGTGGGGTAACGGCTCACCATGGCAATGATACGTAGCCGACCTGAGAGGGTAATCGGCCACATTGGGACTGAGA
CACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCG
TGAGTGAAGAAGGGTTTCGGCTCGTAAAACTCTGTTGTTAAAGAAGAACATATCTGAGAGTAACTGTTCAGGTATTG
ACGGTATTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGG
ATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGTGAAAGCCTTCGGCTCAACCGAAGAAGTGCA
TCGGAAACTGGGAAACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGA
AGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGTATGGGTAGCAAACAGGATT
AGATACCCTGGTAGTCCATACCGTAAACGATGAATGCTAAGTGTTGGAGGGTTTCCGCCCTTCAGTGCTGCAGCTAA
CGCATTAAGCATTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGG
TGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATACTATGCAAATCTAAGAGATTA
GACGTTCCCTTCGGGGACATGGATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC
CCGCAACGAGCGCAACCCTTATTATCAGTTGCCAGCATTAAGTTGGGCACTCTGGTGAGACTGCCGGTGACAAACCG
GAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAA
CGAGTTGCGAACTCGCGAGAGTAAGCTAATCTCTTAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTAC
ATGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG
TCACACCATGAGAGTTTGTAACACCCAAAGTCGGTGGGGTAACCTTTTAGGAACCAGCCGCCTAAGGTGGGACAGAT
GATTAGGGTGAAGTCGTAACAAG
The present invention also provides the exocellular polysaccharide of above-mentioned lactobacillus plantarum, preparation methods are as follows: by Lactobacillus
Plantarum 18-2 is inoculated in the MRS culture medium containing 2% sucrose, centrifuging and taking supernatant, is extracted by alcohol precipitation water extraction thick
Product EPS, and sterling EPS is obtained by dialysis and Sepharose CL-6B chromatographic column method, the EPS is containing there are two component EPSA
With EPSB (as shown in Figures 2 and 3)；There is polysaccharide characteristic absorption peak (such as Fig. 4 and Fig. 5 institute through infrared spectroscopy detection EPSA and EPSB
Show)；Sour water Xie Houjing high-efficient ion chromatogram detects that EPSA is made of galactolipin, glucose and xylose, molar ratio 1.0:
5.1:3.8, EPSB are made of galactolipin, glucose and xylose, and molar ratio 1.0:3.2:6.8, EPSB is also containing 2.85%
Glucuronic acid (such as Fig. 6 and Fig. 7)；Waters high performance liquid chromatograph GPC method detects that its number-average molecular weight is 5800
Er Dun (as shown in Figure 8 and Figure 9).
Compared with prior art, the invention has the benefit that
Lactobacillus plantarum provided by the invention derives from Inner Mongol pastoral area traditional dairy products, has natural, green, safety
Advantage, the Lactobacillus plantarum 18-2 thallus filtered out and its exocellular polysaccharide EPS after purification are to heavy metal
The adsorption effect of lead is obvious, can be applied in leavening and heavy metal adsorption antidote.
Detailed description of the invention
In order to illustrate the technical solutions in the embodiments of the present application or in the prior art more clearly, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only one recorded in the present invention
A little embodiments are also possible to obtain other drawings based on these drawings for those of ordinary skill in the art.
Fig. 1 is the gram stain microscopy photo of Lactobacillus plantarum 18-2 provided by the invention
(MRS, 1000 ×)；
Fig. 2 is the DEAE Sepharose Fast of Lactobacillus plantarum 18-2 provided by the invention
Flow column chromatographic purifying elution curve；
Fig. 3 is the DEAE Sepharose CL-6B column of Lactobacillus plantarum 18-2 provided by the invention
Chromatographic purifying elution curve；
Fig. 4 is the EPSA infrared spectrogram of Lactobacillus plantarum 18-2 provided by the invention；
Fig. 5 is the EPSA infrared spectrogram of Lactobacillus plantarum 18-2 provided by the invention；
Fig. 6 is the EPSA hydrolysate high-efficient ion chromatogram of Lactobacillus plantarum 18-2 provided by the invention
Figure；
Fig. 7 is the EPSB hydrolysate high-efficient ion chromatogram of Lactobacillus plantarum 18-2 provided by the invention
Figure；
Fig. 8 is the EPSA high-efficient liquid phase chromatogram of Lactobacillus plantarum 18-2 provided by the invention.
Fig. 9 is the EPSB high-efficient liquid phase chromatogram of Lactobacillus plantarum 18-2 provided by the invention.
Specific embodiment
In order to make those skilled in the art more fully understand technical solution of the present invention, below in conjunction with embodiment to this
Invention is further detailed.
The test of embodiment 1Lactobacillus plantarum 18-2 heavy metal tolerance
Lactobacillus plantarum 18-2 is inoculated in the MRS culture medium of different concentration of heavy metal ion gradients
Middle culture for 24 hours, observes the growing state of every strains of lactic acid bacteria, is determined to the highest ion concentration of heavy metal tolerance.The result shows that
(being shown in Table 2), Lactobacillus plantarum 18-2 are resistant to 200mg/L Cu2+, 1100mg/L Pb2+And 200mg/
L Cd2+, there is good application potential.Lactobacillus plantarum 28-2 is resistant to 200mg/L in contrast
Cu2+, 600mg/L Pb2+With 900mg/L Cd2+.Suction of the Lactobacillus plantarum 18-2 thallus to heavy metal lead
Attached effect is obvious.
2 Lactobacillus plantarum of table is to various concentration Pb2+Adsorption rate (unit: %)
The EPS heavy metal lead adsorption test of embodiment 2Lactobacillus plantarum 18-2
It is molten to the EPS that 9mL concentration is 0mg/mL, 1mg/mL, 1.5mg/mL, 2mg/mL, 2.5mg/mL and 3mg/mL respectively
It is 1mg/mL Pb that 1mL concentration is added in liquid2+Solution, with turbula shaker be uniformly mixed be placed in 37 DEG C of thermostat water baths
4h is kept, often to stir it is allowed sufficiently to adsorb therebetween, then under conditions of revolving speed is 4000rpm, is centrifuged with centrifuge
20min takes supernatant to carry out Pb later2+Measurement.As a result, it has been found that 2 components of Lactobacillus plantarum 18-2
EPS is when concentration is 1mg/mL and 1.5mg/ml, to Pb2+Show very high adsorption rate (being shown in Table 3).
3 various concentration EPS of table is to Pb2+Adsorption rate (unit: %)
Therefore, EPSA and EPSB is best to the adsorption effect of lead in the present invention, to Pb when concentration is 1mg/ml concentration2+Suction
Attached rate highest, up to 69.59% and 71.61%, better than the xanthan gum of same concentration and the exocellular polysaccharide of other bacterial strains.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Example documented by technical solution modify perhaps equivalent replacement of some of the technical features but these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.It is described above
Only presently preferred embodiments of the present invention is not intended to limit the invention, and all within the spirits and principles of the present invention, that is done appoints
What modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Sequence table
<110>University of the Inner Mongol
<120>a kind of lactobacillus plantarum and its exocellular polysaccharide
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1478
<212> DNA
<213>lactobacillus plantarum (Lactobacillus plantarum)
<400> 1
atacatgcag tcgaacgaac tctggtattg attggtgctt gcatcatgat ttacatttga 60
gtgagtggcg aactggtgag taacacgtgg gaaacctgcc cagaagcggg ggataacacc 120
tggaaacaga tgctaatacc gcataacaac ttggaccgca tggtccgagt ttgaaagatg 180
gcttcggcta tcacttttgg atggtcccgc ggcgtattag ctagatggtg gggtaacggc 240
tcaccatggc aatgatacgt agccgacctg agagggtaat cggccacatt gggactgaga 300
cacggcccaa actcctacgg gaggcagcag tagggaatct tccacaatgg acgaaagtct 360
gatggagcaa cgccgcgtga gtgaagaagg gtttcggctc gtaaaactct gttgttaaag 420
aagaacatat ctgagagtaa ctgttcaggt attgacggta tttaaccaga aagccacggc 480
taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg 540
gcgtaaagcg agcgcaggcg gttttttaag tctgatgtga aagccttcgg ctcaaccgaa 600
gaagtgcatc ggaaactggg aaacttgagt gcagaagagg acagtggaac tccatgtgta 660
gcggtgaaat gcgtagatat atggaagaac accagtggcg aaggcggctg tctggtctgt 720
aactgacgct gaggctcgaa agtatgggta gcaaacagga ttagataccc tggtagtcca 780
taccgtaaac gatgaatgct aagtgttgga gggtttccgc ccttcagtgc tgcagctaac 840
gcattaagca ttccgcctgg ggagtacggc cgcaaggctg aaactcaaag gaattgacgg 900
gggcccgcac aagcggtgga gcatgtggtt taattcgaag ctacgcgaag aaccttacca 960
ggtcttgaca tactatgcaa atctaagaga ttagacgttc ccttcgggga catggataca 1020
ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag 1080
cgcaaccctt attatcagtt gccagcatta agttgggcac tctggtgaga ctgccggtga 1140
caaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac ctgggctaca 1200
cacgtgctac aatggatggt acaacgagtt gcgaactcgc gagagtaagc taatctctta 1260
aagccattct cagttcggat tgtaggctgc aactcgccta catgaagtcg gaatcgctag 1320
taatcgcgga tcagcatgcc gcggtgaata cgttcccggg ccttgtacac accgcccgtc 1380
acaccatgag agtttgtaac acccaaagtc ggtggggtaa ccttttagga accagccgcc 1440
taaggtggga cagatgatta gggtgaagtc gtaacaag 1478
Claims (7)
1. a kind of lactobacillus plantarum, classification naming is Lactobacillus plantarum, and bacterial strain number is
Lactobacillus plantarum18-2, has been preserved in China General Microbiological culture presevation administrative center, and deposit number is
CGMCC No.17566, the deposit date is on April 12nd, 2019.
2. lactobacillus plantarum described in claim 1 is preparing the application in leavening.
3. a kind of exocellular polysaccharide of lactobacillus plantarum, as secreted by lactobacillus plantarum as described in claim 1.
4. the exocellular polysaccharide of lactobacillus plantarum as claimed in claim 3, which is characterized in that the EPS contain there are two component EPSA and
EPSB, number-average molecular weight are 5800 dalton, and EPSA is made of galactolipin, glucose and xylose, molar ratio 1.0:
5.1:3.8, EPSB are made of galactolipin, glucose and xylose, and molar ratio 1.0:3.2:6.8, EPSB is also containing 2.85%
Glucuronic acid.
5. the exocellular polysaccharide of lactobacillus plantarum as claimed in claim 3, which is characterized in that the EPS is prepared by the following method: will
Lactobacillus plantarum18-2 is inoculated in the MRS culture medium containing 2% sucrose, and centrifuging and taking supernatant passes through alcohol precipitation
Water extraction extracts crude product EPS, and obtains sterling EPS by dialysis and Sepharose CL-6B chromatographic column method.
6. the exocellular polysaccharide of the described in any item lactobacillus plantarums of claim 3-5 is preparing answering in heavy metal adsorption antidote
With.
7. the exocellular polysaccharide of lactobacillus plantarum as claimed in claim 6 is preparing the application in heavy metal adsorption antidote, special
Sign is that the heavy metal is lead.
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| CN114317621A (en) * | 2021-10-15 | 2022-04-12 | 重庆医药高等专科学校附属第一医院(重庆市职业病防治院 重庆市第六人民医院 重庆市中毒控制中心) | Lactobacillus metabolite and preparation method and application thereof |
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| CN114317621A (en) * | 2021-10-15 | 2022-04-12 | 重庆医药高等专科学校附属第一医院(重庆市职业病防治院 重庆市第六人民医院 重庆市中毒控制中心) | Lactobacillus metabolite and preparation method and application thereof |
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