CN109504619B - Lactobacillus plantarum and application thereof - Google Patents
Lactobacillus plantarum and application thereof Download PDFInfo
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- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K2035/11—Medicinal preparations comprising living procariotic cells
- A61K2035/115—Probiotics
Abstract
The invention discloses Lactobacillus plantarum and application thereof, wherein a strain of the Lactobacillus plantarum is PA01, and the preservation number is CGMCC No. 15660. The lactobacillus plantarum is high in safety and tolerance, capable of being fixedly planted and grown in intestinal tracts, capable of adjusting the balance of intestinal flora and capable of playing a probiotic role, capable of being used for preparing food or feed additives, adjusting agents for adjusting intestinal diseases of animals, animal antibiotic substitutes, immunomodulators and pathogenic bacteria bacteriostats, capable of being used for improving the production performance of broiler chickens, good in effect, safe and free of side effects.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum and application thereof.
Background
Salmonellosis is an important zoonosis, the etiological agent of which is salmonella enterobacteriaceae, a gram-negative enterobacterium. The clinical symptoms of the livestock infected by the salmonella aggravate the morbidity or mortality of the livestock or reduce the reproductive productivity of the livestock; and may also be asymptomatic with bacteria, and may cause severe bacterial food poisoning after humans eat these contaminated livestock products. In the process of poultry cultivation, the amount of salmonella in the digestive tract of the poultry is reduced, and the risk of the salmonella entering a meat processing plant can be reduced.
Because the addition of antibiotic growth promoters in animal production is limited, in order to effectively control the harm of salmonella, people have been exploring the substitute of antibiotics, and probiotics can promote the development of beneficial microflora of a host, competitively exclude the colonization of harmful pathogenic bacteria, enhance the immunity of mucous membranes, improve the digestive tract digestibility, reduce the pH, and enhance the maturity and integrity of intestinal tissues. Lactic acid bacteria are the first probiotics discovered, and are increasingly widely applied in the livestock industry as a micro-ecological feed additive, and the lactic acid bacteria competitively exclude the colonization of pathogenic bacteria in the intestinal tract of a host by adhering to the surface of epithelial cells of the intestinal tract of the host, strengthen the immune regulation response of the host and effectively control the infection of the poultry by the salmonella. Therefore, lactic acid bacteria are an effective alternative to control salmonella in the poultry industry. Although lactic acid bacteria are a very important probiotic of the animal's intestinal tract itself, their population is often reduced by external influences. Therefore, the lactic acid bacteria can be supplemented through an external source, the dominant flora structure of the lactic acid bacteria in the intestinal tracts of animals is maintained, and the screening of the lactic acid bacteria with better probiotic characteristics is more important.
Disclosure of Invention
Therefore, the invention aims to provide a Lactobacillus plantarum PA01 strain which has excellent acid resistance and cholate resistance, has a wide antibacterial spectrum and can effectively promote the growth of poultry, and the Lactobacillus plantarum PA01 strain is preserved in the China general microbiological culture collection center in 2018, 4 and 28 months, and has the preservation number of CGMCC No. 15660.
The Lactobacillus plantarum PA01 is found in lactic acid bacteria separated from traditional pickle soaking liquid in the region of Yangtze region in Shaanxi.
The Lactobacillus plantarum (Lactobacillus plantarum) PA01 has the following biological properties:
the characteristics of the thallus are as follows: gram-positive bacteria, short rod in the round end, single, paired or chain arrangement, no flagellum and no spore production. 0.6 to 1.0 μm × 2.0 to 5.0 μm.
Colony characteristics: milky white round bacterial colony is neat in edge, smooth and moist in surface and 1.2-3.0 mm in protrusion.
Growth characteristics: the lag phase was about 4h, and the stationary phase was reached after 16h of culture. The optimal growth temperature is 28-35 ℃, the minimum growth temperature of the strain is 15 ℃, and the maximum growth temperature of the strain is 40 ℃. The optimum growth initial pH was 6.5, and the highest and lowest initial growth pH were tolerated at 8.5 and 2.0.
The Lactobacillus plantarum (Lactobacillus plantarum) PA01 also has the following beneficial properties:
broad antibacterial spectrum: can inhibit the growth of escherichia coli, staphylococcus aureus, salmonella enteritidis and salmonella typhimurium, and especially has strong bacteriostatic property on salmonella.
Has the following acid resistance: the biological enzyme can well survive in an acidic environment with the pH value of 2.5, and the activity is almost consistent with that in a normal environment.
Has stronger bile salt resistance: after anaerobic sterile culture at 37 ℃ for 4h in MRS medium containing 0.1%, 0.3%, 0.5% and 1.0% bile salts, the concentration was substantially consistent with the initial concentration.
Moderate adhesion capacity: the adhesion rate to Caco-2 cells was 3.6%.
Has stronger capability of inhibiting the adhesion of salmonella enteritidis to epithelial cells: the competitive adhesion method is adopted to detect the adhesion of the lactobacillus plantarum PA01 to the epithelial cells in the inhibition of the salmonella enteritidis. Adhesion of Salmonella enteritidis in the rejection test and the competition test was 1.67% and 3.5% respectively of the adhesion rate without the addition of Lactobacillus plantarum PA 01.
Has the capability of producing exopolysaccharides: the content of extracellular polysaccharide secreted by the lactobacillus plantarum PA01 can reach 520 mg/L.
The culture method of the lactobacillus plantarum PA01 comprises the following steps: MRS solid culture medium, anaerobic environment, 37 degrees C cultured for 15 ~ 48 h.
The invention also provides application of the lactobacillus plantarum PA01, which comprises a regulator for preparing food or feed additives and regulating animal intestinal diseases, an animal antibiotic substitute, an immunomodulator, a pathogenic bacterium bacteriostatic agent and is also used for improving the production performance of broiler chickens.
The pathogenic bacteria are animal-derived pathogenic bacteria including Escherichia coli, Staphylococcus aureus, Salmonella enteritidis and Salmonella typhimurium.
The invention has the beneficial effects that:
1. the lactobacillus plantarum is separated from the traditional pickle soaking solution, has edible plant source, and fully ensures the application safety of the lactobacillus plantarum in livestock breeding and animal feed; the tolerance is strong, the low pH and the high bile salt concentration of the gastrointestinal tract can be tolerated, and the intestinal tract flora balance can be adjusted and the probiotic effect can be exerted through field planting and growth and propagation in the intestinal tract.
2. The lactobacillus plantarum provided by the invention has obvious bacteriostatic action on escherichia coli, staphylococcus aureus, salmonella enteritidis and salmonella typhimurium, particularly has better bacteriostatic effect on salmonella enteritidis and salmonella typhimurium, has better adhesion effect on salmonella enteritidis, reduces the possibility of contact of harmful bacteria and intestinal epithelial cells, and improves host immunity and non-immune defense systems.
3. The lactobacillus plantarum provided by the invention obviously improves the production performance of broiler chickens, can replace or partially replace antibiotics, and is expected to reduce the addition of the antibiotics serving as long-term sub-therapeutic doses acting on the feed for preventing animal diseases, and has no side effect.
Biological material preservation instructions
And (3) classification and naming: lactobacillus plantarum (Lactobacillus plantarum); the strain number is as follows: PA 01.
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Address: xilu No.1 Hospital No. 3 of Beijing market facing Yang district
The preservation date is as follows: 28 days 4 month 2018
Registration number of the preservation center: CGMCC No. 15660.
Drawings
FIG. 1 is an electrophoresis diagram of Lactobacillus plantarum PA 0116 SrDNA gel identification; wherein, Lane M is DNA molecular weight standard, and Lane 1-6 is Lactobacillus plantarum PA 0116 SrDNA.
Detailed Description
The present invention is further described with reference to the following specific examples, but the scope of the invention is not limited to the examples, and any other products in various forms can be obtained by anyone in the light of the present invention, and any changes in the shape or the component ratio thereof, which have the same or similar technical solutions as the present application, fall within the scope of the present invention. The materials and devices used in the present invention are commercially available unless otherwise specified.
EXAMPLE 1 isolation and purification of the Strain
Lactobacillus plantarum PA01 is found in lactic acid bacteria isolated from traditional pickle soaking liquid in Yangling region of Shaanxi province.
Collecting 50g of the obtained fermented sauerkraut under aseptic condition, and diluting with PBS solution with pH of 7.4 by 10 times gradient with dilution gradient of 102～106After dilution, 200. mu.L of each solution was applied to MRS solid medium (containing 2.0% CaCO)3) And performing inverted culture at 37 ℃ for 48h in an anaerobic environment. Then single colonies with different calcium-dissolving ring sizes and morphologies are picked and inoculated on an MRS liquid culture medium, and cultured overnight at 37 ℃. Uniformly mixing a liquid culture medium, dipping a small amount of bacterial liquid in an inoculating loop in an ultra-clean workbench, streaking the bacterial liquid onto an MRS solid culture medium, carrying out inverted culture for 36-48 h in an anaerobic environment at 37 ℃, completing primary purification, and generally purifying for 3-4 times to ensure that the colony forms on the flat plate are consistent. And uniformly mixing the purified bacterium liquid with sterile 50% glycerol in equal proportion, and placing in an ultra-low temperature refrigerator for later use.
The genome of the strain was extracted and then the strain 16SrDNA gene was amplified using the universal primers 27F and 1492R. Primer 27F was: AGAGTTTGATCCTGGCTCAG, primer 1492R is: GGTTACCTTGTTACGACTT. The PCR reaction conditions are as follows: 10min at 95 ℃; 30s at 95 ℃; 30s at 55 ℃; 90s at 72 ℃; 30 cycles, extension at 72 ℃ for 10 min. FIG. 1 is an electrophoresis diagram of the Lactobacillus plantarum PA 0116 SrDNA gel identification. The 1500bp 16SrDNA sequence (shown as SEQ ID NO.1 in the sequence table) of the strain is compared with the Lactobacillus plantarum 16S rDNA sequence obtained from a GenBank database, and the similarity is 97 percent.
Example 2 detection of bacteriostatic ability of Lactobacillus plantarum PA01
After the pathogenic bacteria are shake-cultured for 10 hours, inoculating the pathogenic bacteria into an LB solid culture medium at the temperature of about 45 ℃ in an inoculation amount of 0.1 percent; placing the sterilized and dried oxford cup in a culture dish; adding about 25mL of LB solid medium containing pathogenic bacteria to each dish; standing at room temperature for 1h, clamping an oxford cup by using sterilized tweezers on the premise of not damaging the structure of a culture hole, and then placing in an ultraclean workbench for 1h at room temperature; adding 200 mu L of lactobacillus bacterial liquid cultured for 20h into the culture hole, culturing overnight at 37 ℃ after the bacterial liquid is completely absorbed at room temperature, measuring the diameter of the inhibition zone, and expressing the inhibition effect of lactobacillus by the diameter of the inhibition zone.
Lactobacillus plantarum PA01 is capable of inhibiting the growth of e.coli (s.aureus ATCC 29213), Salmonella (s.enteritidis ATCC 13076) and Salmonella typhimurium (s.typhimurium ATCC 14082)) and staphylococcus aureus (s.aureus ATCC 29213) when these are used as indicator bacteria. As shown in Table 1, the MRS culture medium has no bacteriostatic effect on various intestinal pathogenic bacteria, and the Lactobacillus plantarum PA01 can strongly inhibit the growth of Escherichia coli, Staphylococcus aureus, Salmonella enteritidis and Salmonella typhimurium.
TABLE 1 Lactobacillus plantarum PA01 bacteriostatic circle diameter (mm)
Example 3 detection of Lactobacillus plantarum PA01 against the gastrointestinal environment
Acid resistance test
To a concentration of about 108～109CFU/mL of 150. mu.L Lactobacillus plantarum suspension was added to 4.85mL of acidic MRS liquid medium pH 2.5; uniformly mixing the culture medium, and then standing and culturing for 3h in a sterile and anaerobic environment at 37 ℃; 10-fold dilution of the Lactobacillus plantarum PA01 suspension to 105～106CFU/mL, respectively adding 500 mu L of lactobacillus plantarum suspension into the culture dish; pouring the MRS solid culture medium cooled to about 45 ℃ into a culture dish, mixing uniformly, naturally cooling for 30min, culturing in an anaerobic sterile environment at 37 ℃ for 48h, and detecting the concentration of lactobacillus plantarum to be 108.41CFU/mL, and maintenance of Normal culture 108.65CFU/mL was nearly as active.
Detection of bile salt resistance
To a concentration of about 108～109CFU/mL of 150. mu.L Lactobacillus plantarum suspension was added to 4.85mL of MRS liquid medium containing 0.1%, 0.3%, 0.5% and 1.0% bile salts, respectively; mixing, standing in 37 deg.C anaerobic sterile environmentCulturing for 4 h; 10-fold dilution of the Lactobacillus plantarum PA01 suspension to 105～106CFU/mL, respectively adding 500 mu L of lactobacillus plantarum suspension into the culture dish; and pouring the MRS solid culture medium cooled to about 45 ℃ into a culture dish, uniformly mixing, naturally cooling for 30min, and culturing for 48h in an anaerobic sterile environment at 37 ℃. The concentrations of the detected lactobacillus plantarum PA01 were 10 respectively8.39，108.11，107.85And 107.62CFU/mL, the strain has stronger bile salt tolerance.
Detection of adhesive Capacity
The method for labeling the lactobacillus plantarum by Fluorescein Isothiocyanate (FITC, Sigma, USA) is adopted to detect the adhesion capacity of the lactobacillus plantarum PA01 to human intestinal Caco-2 epithelial cells.
Centrifuging at room temperature to collect Lactobacillus plantarum PA01, washing the thallus with PBS for 3 times, resuspending the thallus, adding FITC (Sigma, USA) at a ratio of 1:50, and incubating at 37 deg.C in dark for 2 h; centrifuging, removing supernatant, washing with PBS for 3 times, resuspending thallus, and diluting to 5 × 108CFU/mL to obtain fluorescence-labeled Lactobacillus plantarum PA01 bacterial liquid.
The 24-well cell culture plates that had grown a monolayer of cells were washed 2 times with PBS, then 600 μ L of antibiotic-free cell culture medium was added to each well and cultured for 2 h; adding 100 mu L of marked lactobacillus plantarum PA01 bacterial liquid into a 24-pore plate cell culture plate, and culturing for 2 h; washing the mixed solution of the culture medium and the fluorescence-labeled lactobacillus plantarum with PBS for 3 times; then adding 200 mu L of pancreatin into each hole, digesting for 5min, and then adding 600 mu L of PBS; uniformly blowing and mixing the cells in the 24-well plate by using a pipette gun, transferring the cells into a 96-well plate, wherein each well is 200 mu L, and measuring the fluorescence value to be A1; then taking 100 mu L of fluorescence labeled lactobacillus plantarum PA01 bacterial liquid, adding 200 mu L of pancreatin and 500 mu L of PBS, mixing uniformly, subpackaging into 96-well plates with 200 mu L of each well, and determining the fluorescence value to be A2; mixing 200 μ L pancreatin and 600 μ L PBS, adding into 96-well plate, 200 μ L each, and measuring fluorescence value A0; the calculation formula of the adhesion rate of the lactobacillus plantarum is as follows: the adhesion rate was (a1-a0)/(a2-a0) × 100%.
All manipulations need to be performed in the dark to reduce the effect of light on fluorescein. The adhesion rate of the lactobacillus plantarum PA01 to Caco-2 cells is 3.6%, and the lactobacillus plantarum PA01 has moderate adhesion capacity.
Example 4 detection of inhibition of adhesion of Salmonella enteritidis to epithelial cells
The competitive adhesion method is adopted to detect the adhesion of the lactobacillus plantarum PA01 to the epithelial cells in the inhibition of the salmonella enteritidis.
Caco-2 cells were seeded into 24-well cell culture plates, and double-antibody-free DMEM medium (10% fetal bovine serum) was added to 24-well cells, and the mixture was incubated at 37 ℃ for CO2The cells were cultured in a 5% cell culture chamber until a monolayer of cells was formed in the cell culture plate. Mixing Lactobacillus plantarum PA01 and Salmonella, culturing overnight, centrifuging at 8000 Xg for 5min, collecting bacteria, and washing with PBS for 2 times. After centrifugation of the culture solution again, the suspension was suspended in a double-antibody-free DMEM medium (about 1X 10)7cfu/250. mu.L). After removal of Caco-2 cell culture medium, the cells were washed again 2 times with non-anti-PBS.
Rejection test: adding 250 mu L of Lactobacillus plantarum PA01 suspended in a DMEM medium into each 24-hole cell culture plate, incubating for 1h at 37 ℃ in a bacterial incubator, washing for 2 times by using nonreactive PBS, removing the non-adhered Lactobacillus plantarum PA01, adding 0.25% of pancreatin for digestion, adding DMEM containing serum into the culture plate after the cells are basically exfoliated, stopping digestion, collecting liquid, diluting with PBS in a gradient manner, coating the liquid with salmonella selective bright green agar, incubating for 18-24 h at 37 ℃ in the bacterial incubator, and counting.
And (3) competitive test: simultaneously adding lactobacillus plantarum and salmonella enteritidis, co-incubating for 1h under the condition of a bacteria incubator at 37 ℃, and performing subsequent operations and rejection tests. Replacement test: adding salmonella enteritidis, incubating in a bacterial incubator at 37 ℃ for 1h, adding 250 mu L of Lactobacillus plantarum PA01 suspended in DMEM medium, incubating for 1h, and performing subsequent rejection tests.
The salmonella enteritidis adhesion percentage formula: the adhesion rate was a1/a0 × 100%.
Wherein A1 is the number of bacteria of Salmonella enteritidis adhered to cells after lactobacillus plantarum PA01 is added; a0 is the number of bacteria of Salmonella enteritidis adhered to the cells without Lactobacillus plantarum PA 01.
In a rejection test, the lactobacillus plantarum PA01 obviously reduces the adhesion of salmonella enteritidis, which is only 1.67% of the adhesion rate when no lactobacillus plantarum PA01 is added; in a competitive test, the adhesion rate of the salmonella enteritidis is 3.5 percent of that of the salmonella enteritidis without adding the lactobacillus plantarum PA01, and the adhesion of the salmonella enteritidis is also obviously reduced; in the replacement test, the adhesion rate of the salmonella enteritidis was 92.9% of the adhesion rate without the addition of lactobacillus plantarum PA01, although the adhesion of the salmonella enteritidis was reduced but the difference was not significant.
Example 5: ability of lactobacillus plantarum PA01 to produce exopolysaccharides
Inoculating activated lactobacillus plantarum PA01 in an inoculum size of 1.0% into 100ml of MRS culture medium, statically culturing at 37 ℃ for 24h in an anaerobic environment, centrifuging at 10000 Xg for 10min at room temperature, collecting supernatant, adding equal-volume absolute ethyl alcohol placed at 4 ℃ into the supernatant, fully and uniformly mixing, placing in a refrigerator at 4 ℃, standing overnight, centrifuging at 14000 Xg for 5min at room temperature, discarding the supernatant, collecting precipitate, weighing the weight of the collected extracellular polysaccharide, recording the result, and finally converting the result into mg/L.
The exopolysaccharide of the lactobacillus is secreted into the environment in the growth and metabolism process of the lactobacillus, and has important significance for the survival and growth of the lactobacillus. The exopolysaccharide secreted by the lactobacillus can enhance the immunoregulation function of a host, and the secretion quantity of the exopolysaccharide is positively correlated with the immunoregulation capability of the lactobacillus. Analysis shows that the content of extracellular polysaccharide secreted by the lactobacillus plantarum PA01 can reach 520 mg/L.
Example 6 protective Effect of Lactobacillus plantarum PA01 on Salmonella enteritidis infection in broiler chicks
Randomly dividing 72 AA broiler chickens of 1 day old into 3 groups (6 repeats in each group and 4 repeats in Suyangyang city, Shaanxi province), wherein the 1 st group is a negative control group (CON), and feeding 24 broiler chickens with PBS buffer solution (0.2 mL/chicken) once every day in a gastric lavage manner; the second group was a positive control group (SE), 24 broilers were treated with Salmonella enteritidis (10) on day 48～109CFU/0.2 mL/one) infection; the third group was a test group (SE + PA01) fed with Lactobacillus plantarum PA01 (10) once a day9CFU/0.2 mL/mouse), Salmonella (10) on day 48～109CFU/0.2 mL/infection). Broiler livers, spleens and ceca (containing contents) were collected on three groups, day 1, day 3 and day 5 after salmonella infection for salmonella count detection.
Compared with the positive control group, the numbers of the infected salmonella caecum of the experimental group at 1d, 3d and 5d are obviously reduced (see table 2), and the lactobacillus plantarum PA01 can obviously reduce the colonization of the salmonella in caecum (P < 0.05).
Salmonella was detected in the broiler liver and spleen 1, 3 and 5 days post infection, indicating that salmonella was able to invade these tissues rapidly. And no salmonella is detected in the liver and spleen of the broiler chicken infected with salmonella and fed with lactobacillus, which shows that the lactobacillus plantarum PA01 can effectively inhibit infection of the tissues by the salmonella.
TABLE 2 number of Salmonella in cecum, liver and spleen of broiler chickens
Note: the same column is marked with significant differences (P <0.05) for different lower case letters, indicating no detection, the table is the same.
Example 7 Effect of Lactobacillus plantarum PA01 on serum cytokine levels in Salmonella-infected broiler chickens
On the 1 st day, the 3 rd day and the 5 th day after the broiler chicken is infected with salmonella, broiler chicken blood is extracted from a wing artery, transferred to a sterilized centrifugal tube, placed at room temperature for 30min, then centrifuged at low speed for 10min, serum is collected, and then stored in a refrigerator at the temperature of minus 20 ℃. And (3) detecting the titer of the antibody in the sample by adopting an ELISA method.
As shown in Table 3, compared with the positive control group, the Lactobacillus plantarum PA01 test group can significantly reduce the expression levels of proinflammatory cytokines LITAF, IL-1 beta, IL-6 and IL-12 in the blood of the chicks. IL-10 was expressed at a higher level in serum from chicks previously treated with Lactobacillus plantarum PA01 compared to the positive control group (P < 0.05). The anti-inflammatory factor IL-10 in the test group of the lactobacillus plantarum PA01 is improved compared with the positive control group and the negative control group (P <0.05), which indicates that the lactobacillus plantarum PA01 can promote the expression of the anti-inflammatory factor IL-10, activate inflammation and immunosuppressive factors, and regulate inflammatory reaction and immune reaction.
TABLE 3 Effect of Lactobacillus plantarum PA01 on serum cytokine levels in Salmonella-infected broiler chickens
Example 8 Effect of Lactobacillus plantarum PA01 on broiler productivity
288 healthy 1 day old AA broilers were randomly divided into 3 groups of 6 replicates each, 16 broilers per replicate. The group 1 is a control group, and the daily ration is a basic daily ration without antibiotic; the group 2 is antibiotic group, and 50mg/kg of aureomycin is added on the basis of the control group; group 3 was lactobacillus plantarum PA01 addition group (daily ration), 10 was added based on the control group9CFU/kg of Lactobacillus plantarum PA 01. The basic ration is the ration for brooding period in 0-21 days, and the basic ration is the ration for brooding period in 22-42 days.
The average daily gain, average daily feed intake and feed conversion ratio were counted at 42 days of age, and the results are shown in table 4, in which the average daily gain (P <0.05) was significantly increased by the addition of lactobacillus plantarum PA01 to the ration, and was not significantly different from the antibiotic group (P > 0.05). Lactobacillus plantarum PA01 had no significant effect on average daily food intake (P > 0.05). The 42-day-old body weight of the lactobacillus plantarum PA01 group was significantly higher than that of the control group (P <0.05), and was not significantly different from that of the antibiotic group (P > 0.05).
The test result shows that the lactobacillus plantarum PA01 added in the broiler feed has the same effect as the antibiotics, so that the lactobacillus plantarum PA01 can completely replace the antibiotics, thereby reducing the problems of drug resistance and antibiotic residue caused by long-term low-dose antibiotic addition in the diet.
TABLE 4 influence of Lactobacillus plantarum PA01 on broiler productivity
The same row is marked with the same lower case letters with no significant difference (P >0.05) and the same row is marked with different lower case letters with significant difference (P < 0.05).
Sequence listing
<110> northwest agriculture and forestry science and technology university
<120> Lactobacillus plantarum and application thereof
<130> seqlist
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agacccttag gcggctggtt cctaaaaggt taccccaccg actttgggtg ttacaaactc 60
tcatggtgtg acgggcggtg tgtacaaggc ccgggaacgt attcaccgcg gcatgctgat 120
ccgcgattac tagcgattcc gacttcatgt aggcgagttg cagcctacaa tccgaactga 180
gaatggcttt aagagattag cttactctcg cgagttcgca actcgttgta ccatccattg 240
tagcacgtgt gtagcccagg tcataagggg catgatgatt tgacgtcatc cccaccttcc 300
tccggtttgt caccggcagt ctcaccagag tgcccaactt aatgctggca actgataata 360
agggttgcgc tcgttgcggg acttaaccca acatctcacg acacgagctg acgacaacca 420
tgcaccacct gtatccatgt ccccgaaggg aacgtctaat ctcttagatt tgcatagtat 480
gtcaagacct ggtaaggttc ttcgcgtagc ttcgaattaa accacatgct ccaccgcttg 540
tgcgggcccc cgtcaattcc tttgagtttc agccttgcgg ccgtactccc caggcggaat 600
gcttaatgcg ttagctgcag cactgaaggg cggaaaccct ccaacactta gcattcatcg 660
tttacggtat ggactaccag ggtatctaat cctgtttgct acccatactt tcgagcctca 720
gcgtcagtta cagaccagac agccgccttc gccactggtg ttcttccata tatctacgca 780
tttcaccgct acacatggag ttccactgtc ctcttctgca ctcaagtttc ccagtttccg 840
atgcacttct tcggttgagc cgaaaggctt tcacatcaga cttaaaaaac cgcctgcgct 900
cgctttacgc ccaataaatc cggacaacgc ttgccaccta cgtattaccg cggctgctgg 960
cacgtagtta gccgtgcttt ctgttaaata ccgtcaatac tgaacagtac tctcagaata 1020
ttgtttcttc tttaacaaca gagtttttac gagcgaaccg tcttccctca cgcgcgtggc 1080
ttcatcagac ttcgtccatt gtgagatccc ctactgctgc tcggtagagt tcggcggatt 1140
ccagttccaa ttgtgacgga tttaccttac t 1171
Claims (7)
1. A Lactobacillus plantarum (A)Lactobacillus plantarum) The lactobacillus plantarum strain is characterized in that the name of the lactobacillus plantarum strain is PA01, and the preservation number is CGMCC No. 15660.
2. Use of the lactobacillus plantarum of claim 1 for the preparation of food and feed additives.
3. Use of lactobacillus plantarum as defined in claim 1 for the preparation of a modulator for modulating intestinal disorders in an animal.
4. Use of lactobacillus plantarum as defined in claim 1 for the preparation of an antibiotic substitute for animals.
5. Use of lactobacillus plantarum as defined in claim 1 for the preparation of an immunomodulator.
6. Use of a lactobacillus plantarum as claimed in claim 1 for the preparation of a bacteriostatic agent for pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella enteritidis and salmonella typhimurium.
7. The application of the lactobacillus plantarum of claim 1 in improving broiler chicken productivity.
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| CN110106121B (en) * | 2019-05-30 | 2020-12-01 | 江南大学 | Lactobacillus plantarum for producing extracellular polysaccharide |
| CN110835620B (en) * | 2019-12-16 | 2022-08-09 | 吉林农业大学 | Lactobacillus plantarum and application thereof |
| CN111235070B (en) * | 2020-03-18 | 2022-03-08 | 河北农业大学 | Breast milk infant source lactobacillus plantarum BF _15 and application thereof |
| CN111635873B (en) * | 2020-06-04 | 2021-02-02 | 山东宝来利来生物工程股份有限公司 | Lactobacillus plantarum, microecological preparation thereof, and preparation method and application thereof |
| JP2022549015A (en) * | 2020-06-12 | 2022-11-22 | ベイジン サンユアン フーズ カンパニー リミテッド | Lactobacillus plantarum derived from breast milk and uses thereof |
| CN111778180B (en) * | 2020-06-12 | 2021-12-14 | 北京三元食品股份有限公司 | Breast milk source lactobacillus plantarum and application thereof |
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| CN112167305B (en) * | 2020-09-27 | 2022-04-22 | 浙江青莲食品股份有限公司 | Cleaning method for removing peculiar smell of pig large intestine |
| CN112322524A (en) * | 2020-10-29 | 2021-02-05 | 青岛普罗百世生物科技有限公司 | Lactobacillus plantarum and application thereof in broiler feed |
| CN112852684B (en) * | 2021-04-02 | 2022-04-01 | 湖北均瑶大健康饮品股份有限公司 | Lactobacillus plantarum strain Y388 and application thereof |
| CN114561436A (en) * | 2022-02-28 | 2022-05-31 | 西北农林科技大学 | Preparation method and application of lactobacillus plantarum exopolysaccharide |
| CN115886149A (en) * | 2023-01-06 | 2023-04-04 | 吉林农业科技学院 | Application of lactobacillus plantarum LPJZ-658 in improving growth performance, meat quality and disease resistance of broiler chickens |
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