CN114426934B - Lactobacillus plantarum for detecting biotoxicity of source wastewater and application thereof - Google Patents
Lactobacillus plantarum for detecting biotoxicity of source wastewater and application thereof Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention relates to the technical field of sewage treatment, and discloses lactobacillus plantarum for detecting biotoxicity of source wastewater and application thereof. The Lactobacillus plantarum is named CR3 and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) for 3 months and 15 days in 2021, and has an address of No. 1 and No. 3 of North Chen West Lu in the Korean area of Beijing, and a preservation number of CGMCC No.22011, and a microorganism classification of the Lactobacillus plantarumLactobacillus plantarum. The lactobacillus plantarum CR3 can hydrolyze esculin to generate black substances, has proper tolerance to wastewater toxicity, can intuitively reflect the strength of wastewater toxicity through color development, has obvious and stable color development, and can be used for detecting the biological toxicity of source wastewater.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to lactobacillus plantarum for detecting biotoxicity of source wastewater and application thereof.
Background
Along with the rapid development of the industry in China, the types of pollutants in water bodies are more and more complex. After the concentration of a plurality of special pollutants reaches a certain threshold, the biological treatment of the wastewater is inhibited, even destroyed, such as heavy metal, chloroaniline, cyanide, nitrobenzene and the like, and the water quality condition of the wastewater can not be objectively and accurately evaluated only by using conventional water quality indexes such as COD, BOD, TN, TP, so that the current situation of the current industrial wastewater treatment can not be satisfied by performing quality splitting only according to the conventional water quality indexes, and the source quality splitting based on the wastewater toxicity is needed.
Biotoxicity detection techniques (Bio-toxicity Tests) are methods for comprehensively evaluating the toxicity of substances by evaluating their response to organisms. Compared with a physicochemical method, the biological method can evaluate the influence of unknown toxic and harmful pollutants in the wastewater on the living beings, and can reflect complex interactions among a plurality of pollutants in the wastewater and the bioavailability of the pollutants, so that the biological method plays an important role in the control and management of the toxicity of the wastewater, can be used for searching the safety concentration of certain chemical substances or industrial wastewater on the living beings, provides scientific basis for formulating reasonable water quality standards and wastewater discharge standards, can also be used for evaluating the effective degree of wastewater treatment, and screens proper industrial wastewater toxicity reduction technology.
In industrial wastewater treatment, conventional activated sludge is a necessary treatment unit, and the nature of activated sludge is microorganism, so that a bacterial method is most suitable when developing a toxicity detection technology suitable for industrial wastewater to a rear-end conventional activated sludge treatment system. MIC (Minimum inhibitory concentration ) refers to the minimum drug concentration capable of inhibiting macroscopic growth of bacteria to be tested, originally applied to measurement of drug sensitivity, so as to accurately and effectively utilize the drug for treatment; and is also a traditional measure for measuring the efficacy of antibiotics on various pathogenic bacteria. The MIC biotoxicity detection technology is applied to wastewater biotoxicity detection, and the toxicity of the wastewater to microorganisms can be judged by gradient dilution of the wastewater, mixing the wastewater with a bacterial culture medium, and culturing for a period of time to find the lowest wastewater concentration capable of inhibiting bacterial growth in the culture medium. For example, patent CN201910403966.2 discloses a distinguishing management method of pharmaceutical wastewater based on MIC toxicity detection technology, which adopts G with first abundance ratio in activated sludge + The standard strains of bacteria and G-bacteria can judge the toxicity of the wastewater by observing the growth conditions of the two strains in wastewater with different dilutions, but the growth conditions of the strains can only be reflected by the abundance of the strains in the wastewater, and are difficult to observe intuitively.
Disclosure of Invention
In order to solve the technical problems, the invention provides lactobacillus plantarum for detecting biotoxicity of source wastewater and application thereof. The lactobacillus plantarum CR3 can hydrolyze esculin to generate black substances, has proper tolerance to wastewater toxicity, can intuitively reflect the strength of wastewater toxicity through color development, has obvious and stable color development, and can be used for detecting the biological toxicity of source wastewater.
The specific technical scheme of the invention is as follows:
in a first aspect, the invention provides a lactobacillus plantarum for detecting biotoxicity of source wastewater, wherein the lactobacillus plantarum is named CR3 and is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) for 3 months and 15 days in 2021, the address is North Chen West Lu No. 1, the Korean region of Beijing city, the preservation number is CGMCC No.22011, and the microorganism classification is named lactobacillus plantarum Lactobacillus plantarum.
The lactobacillus plantarum CR3 is easy to culture, can secrete esculin hydrolase, and can decompose esculin into glucose and esculin, the esculin reacts with iron ions to generate black compounds, and the more the lactobacillus plantarum CR3 is, the darker the black is, so that the inhibition effect of wastewater on the lactobacillus plantarum CR3 can be intuitively reflected by observing the color depth. In addition, the lactobacillus plantarum CR3 has proper tolerance to the toxicity of the wastewater, can show the strength of the toxicity of the wastewater, and has obvious and stable color development. Therefore, the lactobacillus plantarum CR3 can be used for detecting biological toxicity of source wastewater, judging the toxicity of the wastewater according to the color development condition of a culture system, and further judging whether the wastewater can directly enter a rear-end biochemical treatment system (usually, the wastewater is introduced into a rear-end comprehensive regulating tank, the wastewater treated by the comprehensive regulating tank is subjected to further toxicity detection, and the wastewater is introduced into a microbial treatment unit such as an activated sludge treatment unit after the wastewater is qualified in detection).
In a second aspect, the invention provides a mutant of lactobacillus plantarum for detecting biotoxicity of source wastewater, wherein the mutant is obtained by mutagenesis, domestication, gene recombination or natural mutation of the lactobacillus plantarum.
In a third aspect, the present invention provides a bacterial culture comprising said lactobacillus plantarum or comprising said mutant.
Preferably, the bacterial culture is a bacterial liquid or a bacterial agent.
In a fourth aspect, the invention provides the use of said lactobacillus plantarum or said mutant in the detection of biotoxicity of source wastewater.
Preferably, the application comprises the steps of:
(1) Adding tryptone, yeast extract, ferric citrate and esculin into water, and mixing to obtain culture medium;
(2) Adding the wastewater into a culture medium to prepare a mixed system; inoculating the lactobacillus plantarum or the mutant into a mixed system to prepare a culture system;
(3) Culturing the culture system, observing the color development condition, if the color development is not performed, the wastewater can not directly enter the rear-end biochemical treatment system, and if the color development is performed, the wastewater can directly enter the rear-end biochemical treatment system.
Preferably, in the step (2), the volume fraction of the wastewater in the mixed system is 25-35%; the inoculation amount of the lactobacillus plantarum or the mutant in the mixed system is 0.1-0.34g/L.
According to experience, the wastewater concentration of 25-35% (i.e. the volume of the wastewater after dilution is 25-35% of the total volume) is taken as a critical point for judging that the toxicity degree of the wastewater is high, and the wastewater concentration is preferably 30%. Namely, when the MIC is less than 25-35%, the wastewater is high in toxicity, has a large influence on the rear-end biochemical treatment system, and cannot directly enter the rear-end biochemical treatment system; when the MIC is more than 25-35%, the wastewater is non-toxic, has small influence on the rear-end biochemical treatment system, and can directly enter the rear-end biochemical treatment system.
Lactobacillus plantarum CR3 can distinguish high-toxic wastewater from non-high-toxic wastewater when the wastewater concentration is 25-35% under the condition that the inoculation amount is 0.1-0.34g/L, namely, the high-toxic wastewater does not appear black when cultured under the condition that the wastewater concentration is 25-35%, and the non-high-toxic wastewater does not appear black. Therefore, the lactobacillus plantarum CR3 can be applied to biological toxicity detection of source wastewater, and can judge whether the wastewater can directly enter a rear-end biochemical treatment system according to the color development condition when the wastewater is cultured at the concentration of 25-35%.
In addition, when the esculin and the ferric citrate are added into the conventional lactobacillus culture medium to culture the lactobacillus plantarum CR3, the culture system cannot develop color, which indicates that the conventional lactobacillus culture medium cannot be used for detecting the biotoxicity of the source wastewater. Therefore, the invention innovates a culture medium formula, and when the culture medium is adopted to culture lactobacillus plantarum CR3, the culture system can develop color, so that the invention can be used for detecting the biotoxicity of the source wastewater.
Preferably, in step (1), the concentrations of tryptone, yeast extract, ferric citrate and esculin in the medium are 3.3-10g/L, 1.67-5g/L, 0.5-0.6g/L and 1-1.2g/L, respectively, more preferably 3.3g/L, 1.67g/L, 0.5g/L and 1g/L.
Too high or too low concentrations of the components in the culture medium can affect the detection of the biotoxicity of the source wastewater, and specifically: when the concentration is too low, the culture system cannot develop color, so that the toxicity of the wastewater cannot be reflected; when the concentration is too high, the nutrient content in the culture medium is too high, so that the influence of the nutrient content in the wastewater on the growth of the strain can be covered. Thus, the concentrations of tryptone, yeast extract, ferric citrate and esculin are set to 3.3-10g/L, 1.67-5g/L, 0.5-0.6g/L and 1-1.2g/L, respectively, within the above ranges, the culture system is capable of developing color; further preferably 3.3g/L, 1.67g/L, 0.5g/L and 1g/L, at which the nutrient content in the medium is low, without masking the effect of the nutrient content in the wastewater on the growth of the strain.
Compared with the prior art, the invention has the following advantages:
(1) The lactobacillus plantarum CR3 can hydrolyze esculin, then generates black substances, has proper tolerance to wastewater toxicity, can intuitively reflect the strength of wastewater toxicity through color development, has obvious and stable color development, and is convenient for source wastewater biotoxicity detection;
(2) The front-end wastewater biotoxicity detection method can judge the high-toxicity wastewater and the non-high-toxicity wastewater by taking the wastewater concentration of 25-35% as a critical point with higher toxicity degree, thereby judging whether the wastewater can directly enter a rear-end biochemical treatment system.
Detailed Description
The invention is further described below with reference to examples.
Example 1: degree of color development of different strains
Through data investigation, a typical species with esculin hydrolase activity is enterococcus. Enterococcus is pathogenic and unsuitable for use. Enterococci belong to the order lactobacillus (Lactobacillales) in the microorganism classification, and it is presumed that lactobacillus species may have similar biochemical characteristics, so model species are focused on lactobacillus.
8 strains of lactic acid bacteria and enterococcus faecalis from different sources are selected as test bacteria, bacterial powder of each strain is inoculated into 2 nutrient media containing color former esculin according to the inoculum size of 1% (namely 0.1 g/L), and the culture media are placed in a constant temperature shaking incubator at 30 ℃ for culturing for 24 hours, and the color development condition is observed, and the result is shown in Table 1.
The formula of the culture medium 1 is as follows: 10g/L of tryptone, 5g/L of Yeast Extract (YE), 0.5g/L of ferric citrate and 1g/L of esculin, wherein the solvent is water; ph=6.2±0.2.
The formula of the culture medium 2 is as follows: peptone 10g/L, beef extract 5g/L, YE 4g/L, glucose 20g/L, K 2 HPO 4 2g/L, triamine citrate 2g/L, sodium acetate 5g/L, mgSO 4 0.2g/L，MnSO 4 0.05g/L, 1g/L Tween 80, 0.5g/L ferric citrate and 1g/L esculin, wherein the solvent is water; ph=6.2±0.2.
TABLE 1 degree of color development of different strains
As can be seen from table 1:
(1) Medium 1 was the medium designed by the inventors and medium 2 was a conventional lactic acid bacteria medium. Strain 1# -8# did not develop color in medium 2, indicating that conventional lactobacillus culture medium could not be used as a medium for lactobacillus in the source wastewater biotoxicity assay (probably due to the presence of some color inhibitor in medium 2).
(2) Different strains have different color development degrees in the culture medium 1, 1# -8# and enterococcus faecalis are gram positive bacteria, and can develop color, 1#, 6#, 7#, 8# are obvious, and 11# is gram negative bacteria and does not develop color, so 1#, 6#, 7# and 8# can be used as pseudomodel strains.
Example 2: effect of salinity on strain growth
Adding NaCl in different amounts into the culture medium 1, inoculating bacterial powder of each strain according to the inoculation amount of 1g/L, placing the strain into a constant-temperature shaking incubator at 30 ℃ for culturing for 24 hours, observing the growth condition of the strain, and quantitatively measuring the turbidity of bacterial liquid at the wavelength of 600nm by using a spectrophotometer, wherein the result is shown in Table 2.
TABLE 2 Effect of salinity on seed growth
The model strain needs to satisfy: can normally grow and develop color in the conventional salinity range (less than or equal to 3 weight percent); that is, this series of techniques does not characterize toxicity over a conventional salinity range (.ltoreq.3 wt%).
As can be seen from table 2: the addition of the salinity has obvious influence on the growth of strains, and the growth amounts of the No. 1 strain and the No. 5 strain are always arranged in the front in the salinity range of 0-3wt%, so that the No. 1 strain and the No. 5 strain can be used as pseudomodel strains.
Example 3: influence of organic matters and inorganic ions on color development
Various substances which possibly have influence on color development in the culture medium 2 are respectively added into the culture medium 1, bacterial powder of each bacterial strain is inoculated according to the inoculation amount of 1g/L, and then the bacterial powder is placed in a constant-temperature shaking incubator at 30 ℃ for culturing for 24 hours, and the color development condition is observed, and the result is shown in Table 3.
TABLE 3 influence of substances on the color development of strains
As can be seen from table 3: the addition of glucose can obviously inhibit the color development, and the addition of sodium acetate is beneficial to the color development, so the detection technology needs to be vigilant whether the water sample contains glucose and sodium acetate. Magnesium sulfate and manganese sulfate have little effect on strain # 1 and strain # 8, so strain # 1 and strain # 8 can be used as pseudomodel strains.
Example 4: influence of wastewater concentration on color development
In combination with the results of example 2 and example 3, 4 strains are preferred: # 1, # 2, # 6, # 8. The waste water with different contents is added into the culture medium 1, the final content of the waste water in the system is 10%, 30% and 50% (v/v), the strain powder of 1# strain, 2# strain, 6# strain and 8# strain are respectively inoculated into the waste water according to the inoculum size of 1g/L, and the waste water is placed into a constant temperature shaking incubator at 30 ℃ for culturing for 24 hours, and the color development condition is observed, and the result is shown in Table 4.
TABLE 4 wastewater MIC results for four strains
| Sequence number | 10％ | 30％ | 50％ |
| 1# | ++ | + | - |
| 2# | ++ | + | - |
| 6# | ++ | - | - |
| 8# | ++ | - | - |
Note that: "+" represents color development and "-" represents no color development.
From table 4, it can be seen that: the No. 1, no.2, no. 6 and No. 8 strains can show the toxicity of the wastewater.
Example 5: identification of strains
The results of examples 1-4 are combined, the strain 1 has obvious color development, the growth amount is maximum, the color development is stable, and the toxicity of wastewater can be reflected. A large number of subsequent wastewater tests show that the toxicity of the 1# bacteria for detecting various types of wastewater is basically consistent with that of the laboratory early detection result. Thus, strain # 1 was selected as a model strain.
The strain # 1 was subjected to 16S rRNA sequencing and aligned for highest homology with the known 16S rRNA gene in GenBank. The identified strain 1 belongs to Lactobacillus plantarum and is designated CR3.
Example 6: effect of sodium acetate on MIC results of wastewater from strain # 1
Adding 5g/L sodium acetate into the culture medium 1 containing esculin and not containing esculin respectively to obtain 2 culture mediums; 3 representative waste water (Lp3#, lp24#, and Lp27#) are selected, waste water with different contents is added into two culture mediums, the final content of the waste water in the system is 10%, 30% and 50% (v/v), 1# strain bacterial powder is inoculated into the waste water according to the inoculation amount of 1g/L, the waste water is placed into a constant temperature shaking incubator at 30 ℃ for culturing for 24 hours, then the bacterial growth condition in a culture system without esculin and the color development condition in a culture system with esculin are observed, and compared with the result of a conventional MIC method (namely the method in patent CN 201910403966.2), and the result is shown in Table 5.
TABLE 5 influence of sodium acetate addition on MIC detection results of wastewater from 1# bacteria
Note that: "+" indicates that the strain is growing, and "-" indicates that the strain is not growing.
From table 5, it can be seen that: the addition of sodium acetate can change the toxicity result of wastewater MIC detection, so that the strain 1 cannot accurately reflect the toxicity of wastewater, and although sodium acetate is beneficial to color development, the subsequent addition of sodium acetate into a culture medium is not considered.
Example 7: optimization of the addition amount of the fungus powder
2 representative waste water (Lp1# and Lp2#) are selected, and waste water with different contents is added into a culture medium 1, so that the final content of the waste water in a system is 10%, 30% and 50% (v/v); respectively adding different amounts of bacterial solutions of the No. 1 strain into the mixed system to obtain culture systems with bacterial concentrations of 1.0g/L, 1.7g/L and 3.4g/L respectively; after the culture system was cultured in a shaking incubator at a constant temperature of 30℃for 24 hours, the color development was observed, and the results are shown in Table 6.
TABLE 6 experimental results of different bacterial powder addition amounts
| Lp1# wastewater | Lp2# wastewater | |
| Results of conventional MIC method | 10％+，30％-，50％- | 10％+，30％-，50％- |
| The concentration of bacteria is 1.0g/L | 30% black green, 50% light green | 30% of black and green, 50% of light green |
| The concentration of bacteria is 1.7g/L | 30% of black and green, 50% of light green | 30% of black and green, 50% of light green |
| The concentration of bacteria is 3.4g/L | 30% of black and 50% of green | 30% of black and 50% of green |
Note that: "+" indicates that the strain is growing, and "-" indicates that the strain is not growing.
As can be seen from table 6: under the three concentrations, the toxicity of the wastewater is inconsistent with the conventional result, and the MIC value is higher, which indicates that the concentration of the bacterial powder is too high. Therefore, the mixed solution of the bacterial powder was further diluted, and a study experiment was performed to reduce the bacterial powder concentration, and the experimental results are shown in table 7.
TABLE 7 Experimental results of different bacterial powder addition amounts
| Lp1# wastewater | Lp2# wastewater | |
| Conventional MIC results | 10％+，30％- | 10％+，30％- |
| The concentration of bacteria is 0.1g/L | 10% of black and green, 30% of light green | 110% of black and green, 30% of light green |
| Bacteria concentration is 0.17g/L | 10% of black and green, 30% of light green | 10% of black and green, 30% of light green |
| Bacteria concentration is 0.34g/L | 10% of black, 30% of green | 10% of black, 30% of green |
Note that: "+" indicates that the strain is growing, and "-" indicates that the strain is not growing.
From table 7, it can be seen that: under the three concentrations, the toxicity of the wastewater is consistent with the conventional result, so that the inoculation amount of the bacterial powder in the invention can be 0.1-0.34g/L.
Example 8: optimization of the concentration of the Medium Components
The concentration of each component in the culture medium 1 is regulated to different degrees, 9 different culture media are obtained, 1# strain bacterial powder is inoculated according to the inoculation amount of 0.1g/L, the culture medium is placed in a constant temperature shaking incubator at 30 ℃ for culturing for 24 hours, the color development condition is observed, and the experimental results are shown in table 8.
TABLE 8 color development results after the concentration of each component in the medium was changed
From table 8, it can be seen that: the strain # 1 was cultured using the two media of experimental groups 1 and 2, which were able to develop black, and therefore, in the source wastewater biotoxicity test using the strain # 1, the two media were selected. The medium of experimental group 2 is preferred because: the nutrient content in the culture medium of the experiment group 1 is larger, and the influence of the nutrient content in the wastewater on the growth of the No. 1 strain is easily covered.
Example 9: biological toxicity detection of source wastewater
The culture medium of experiment group 2 in example 8 was used to dilute the waste water of Lp1#, lp2#, lp3#, lp24#, and Lp27#, and a mixed system with waste water concentrations of 10%, 30%, and 50% was obtained, and the mixed system was inoculated with strain 1# bacterial powder at inoculum sizes of 0.1g/L, 0.17g/L, and 0.34g/L, and after the culture in a 30 ℃ constant temperature shaking incubator for 24 hours, the color development was observed, and compared with the results of the conventional MIC method, and the results are shown in Table 9.
TABLE 9 toxicity detection of wastewater after concentration change
Note that: in the conventional MIC method, "+" represents strain growth and "-" represents strain no growth; in the method of the present invention, "+" represents color development and "-" represents no color development.
From table 9, it can be seen that: when the culture medium formulation of experiment group 2 in example 8 was used and the inoculation amount of strain # 1 was 0.1-0.34g/L, the test results were the same as the conventional MIC method, indicating that the above culture medium formulation and inoculation amount can be used for source wastewater biotoxicity detection.
The raw materials and equipment used in the invention are common raw materials and equipment in the field unless specified otherwise; the methods used in the present invention are conventional in the art unless otherwise specified.
The foregoing description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and any simple modification, variation and equivalent transformation of the above embodiment according to the technical substance of the present invention still fall within the scope of the technical solution of the present invention.
Claims (6)
1. Lactobacillus plantarum for detecting biotoxicity of source wastewater, characterized in that it is named CR3 and has been deposited on month 15 of 2021The collection number is CGMCC No.22011 in China general microbiological culture Collection center, and the microorganism classification is named as lactobacillus plantarumLactobacillus plantarum。
2. A bacterial culture comprising the lactobacillus plantarum of claim 1.
3. The bacterial culture according to claim 2, wherein the bacterial culture is a bacterial liquid or a bacterial agent.
4. Use of lactobacillus plantarum according to claim 1 for the detection of biotoxicity of source wastewater.
5. The use according to claim 4, comprising the steps of:
(1) Adding tryptone, yeast extract, ferric citrate and esculin into water, and mixing to obtain culture medium;
(2) Adding the wastewater into a culture medium to prepare a mixed system; inoculating the lactobacillus plantarum into a mixed system to prepare a culture system;
(3) Culturing the culture system, observing the color development condition, if the color development is not performed, the wastewater can not directly enter the rear-end biochemical treatment system, and if the color development is performed, the wastewater can directly enter the rear-end biochemical treatment system.
6. The use according to claim 5, wherein in step (2), the volume fraction of waste water in the mixed system is 25-35%; the inoculation amount of the lactobacillus plantarum in the mixed system is 0.1-0.34g/L.
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| WO2013082916A1 (en) * | 2011-12-06 | 2013-06-13 | 光明乳业股份有限公司 | Strain of exopolysaccharide-secreting lactobacillus plantarum and application thereof |
| CN105132322A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Lactobacillus plantarum and application thereof |
| CN107058166A (en) * | 2017-01-13 | 2017-08-18 | 东南大学 | The Lactobacillus plantarum of one plant of extracellular polysaccharide |
| CN110205356A (en) * | 2019-05-15 | 2019-09-06 | 杭州秀川科技有限公司 | A kind of differentiated management method of the pharmacy waste water based on MIC toxicity detection technology |
| CN111040960A (en) * | 2019-11-19 | 2020-04-21 | 西南大学 | Lactobacillus plantarum LP33 and application thereof in preparation of product for promoting lead excretion |
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| WO2013082916A1 (en) * | 2011-12-06 | 2013-06-13 | 光明乳业股份有限公司 | Strain of exopolysaccharide-secreting lactobacillus plantarum and application thereof |
| CN105132322A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Lactobacillus plantarum and application thereof |
| CN107058166A (en) * | 2017-01-13 | 2017-08-18 | 东南大学 | The Lactobacillus plantarum of one plant of extracellular polysaccharide |
| CN110205356A (en) * | 2019-05-15 | 2019-09-06 | 杭州秀川科技有限公司 | A kind of differentiated management method of the pharmacy waste water based on MIC toxicity detection technology |
| CN111040960A (en) * | 2019-11-19 | 2020-04-21 | 西南大学 | Lactobacillus plantarum LP33 and application thereof in preparation of product for promoting lead excretion |
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