CN107058141B - A kind of microbial bacterial agent and its application - Google Patents
A kind of microbial bacterial agent and its application Download PDFInfo
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- CN107058141B CN107058141B CN201710185202.1A CN201710185202A CN107058141B CN 107058141 B CN107058141 B CN 107058141B CN 201710185202 A CN201710185202 A CN 201710185202A CN 107058141 B CN107058141 B CN 107058141B
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- strawberry
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- saccharomycete
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- 230000000813 microbial effect Effects 0.000 title claims description 27
- 230000001580 bacterial effect Effects 0.000 title claims description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- 235000016623 Fragaria vesca Nutrition 0.000 claims abstract description 23
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims abstract description 23
- 230000000694 effects Effects 0.000 claims abstract description 21
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 15
- 239000000725 suspension Substances 0.000 claims abstract description 14
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 claims abstract description 11
- 244000253911 Saccharomyces fragilis Species 0.000 claims abstract description 11
- 235000018368 Saccharomyces fragilis Nutrition 0.000 claims abstract description 11
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 11
- 229940031154 kluyveromyces marxianus Drugs 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 210000001082 somatic cell Anatomy 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 2
- 240000009088 Fragaria x ananassa Species 0.000 claims 1
- 239000008223 sterile water Substances 0.000 claims 1
- 241000220223 Fragaria Species 0.000 abstract description 22
- 244000005700 microbiome Species 0.000 abstract description 20
- 239000000126 substance Substances 0.000 abstract description 11
- 239000000203 mixture Substances 0.000 abstract description 10
- 239000005740 Boscalid Substances 0.000 abstract description 9
- WYEMLYFITZORAB-UHFFFAOYSA-N boscalid Chemical compound C1=CC(Cl)=CC=C1C1=CC=CC=C1NC(=O)C1=CC=CN=C1Cl WYEMLYFITZORAB-UHFFFAOYSA-N 0.000 abstract description 9
- 229940118790 boscalid Drugs 0.000 abstract description 9
- 238000009472 formulation Methods 0.000 abstract description 9
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 7
- 239000003899 bactericide agent Substances 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000013329 compounding Methods 0.000 abstract description 4
- 230000002265 prevention Effects 0.000 abstract description 4
- 208000031968 Cadaver Diseases 0.000 abstract description 2
- 230000003449 preventive effect Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000002068 microbial inoculum Substances 0.000 abstract 1
- 238000005507 spraying Methods 0.000 abstract 1
- 239000000575 pesticide Substances 0.000 description 16
- 238000011161 development Methods 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000447 pesticide residue Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 239000004562 water dispersible granule Substances 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010068052 Mosaicism Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000003765 sex chromosome Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Dentistry (AREA)
- Genetics & Genomics (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to biological fields, and in particular to be using saccharomycete kluyveromyces marxianus microbial inoculum prevention corps diseases, especially grey mould fruit rot of strawberry purposes.The main component of the microorganism formulation of the present invention is kluyveromyces marxianus, by being prepared into bacteria suspension spraying prevention grey mould fruit rot of strawberry to strain, preventive effect is more than 45%, and when it is used in compounding with chemical bactericide Boscalid, it is possible to reduce the usage amount of chemical bactericide and maintain its preferable control effect.The microorganism formulation raw material of the present invention is easy to get, and preparation process is simple, environmentally safe using the microorganism formulation, green safe, and Social benefit and economic benefit is apparent after promoting.
Description
Technical field
The invention belongs to microorganism fields, and in particular to be it is a kind of for preventing the microorganism formulations of corps diseases,
The microorganism formulation is safe to the human body in the application, nuisanceless to environment.
Background technology
Biological pesticide refers to each for preventing using living microorganism, botanical pesticide, Secondary Metabolites of Microorganisms etc.
Kind insect and the pesticide variety of disease.Especially microbial pesticide, since microbial resources type potential in nature is enriched,
There is apparent advantage in terms of the product development of microbial pesticide, therefore, countries in the world have all put into a large amount of manpower and wealth
Power is studied, and in China, the development and exploitation of microbial pesticide have become national science and technology public relations and hi-tech development
Plan；Each place government is also using microbial pesticide as a research direction of biological field.
Microbial pesticide is the important guarantor for realizing development proposal adjustment, promoting circular economy and social sustainable development
Barrier, therefore, novel microbial pesticide of the research and development with independent intellectual property right, and other high-tech industries are driven with this, such as
The cooperative development of Fermentation Engineering, preparation processing, field of fine chemical is an important goal of microbial pesticide development.Microorganism
Pesticide not only have the characteristics that efficiently, it is safety, low-residual, degradable, but also microorganism in itself from environment in itself, to environment
Close friend, and microbial action complicated mechanism, action site is more, and general microorganism live bacteria and secondary metabolite can be applied to
Inhibit the growth of harmful organism, thus receive people's concern.At present, actinomyces, bacterium etc. are agriculturally being widely popularized
It uses, although however, carrying out biological control using saccharomycete has had research, that really applies is also less.
Saccharomycete is generally larger to chemical bactericide resistance or even can be used in combination with chemical bactericide, once it screens
To suitable compounding type, the dosage of chemical pesticide can not only be reduced, and can reduce potential to environment and human body
While causing harm, biological control effect is improved.In addition, saccharomycete has, inheritance stability, nutritional requirement is simple, antimicrobial spectrum is wide, raw
Long fireballing feature and stronger tolerance, energy insensitive to most chemical bactericides under various abiotic stress environment and adverse circumstance
With some, chemically or physically method is combined, and becomes the hot spot of microbial control research.
Strawberry is a kind of perennial root, perennial herb resource plant for integrating cultivation and watching.In cultivated strawberry area,
Gray mold, anthracnose, powdery mildew are to hinder the bottleneck of Strawberry industry development, wherein, the harm of grey mould fruit rot of strawberry is the most serious,
Annual strawberry growth period endangers stem, petiole, blade, stipule, the later stage endanger flower and fruit, directly influence yield of strawberry,
Cause very big economic loss.Most of prevention to grey mould fruit rot of strawberry causes soil using chemical pesticide sprinkling plant surface
Different degrees of pesticide residue and pollution problem seriously affect product quality and the health of human body.From preserving the ecological environment
It is punished with the angle of food security, strawberry production needs low toxicity, pollutes few, safe to the human body microorganism formulation to control grass
Certain kind of berries disease so as to achieve the purpose that strawberry safe edible, also complies with biological husbantry development and government, society and biological pesticide industry
Development.
Invention content
Situation about being impacted it is an object of the invention to be directed to grey mould fruit rot of strawberry to strawberry, provides a kind of microorganism live bacteria
Preparation, so as to solve largely to use pesticide residue, Environmental security sex chromosome mosaicism caused by chemical pesticide in the prior art.
Another object of the present invention is to provide the application of the microorganism live bacteria preparation, the microorganism live bacteria preparation is fitted
For preventing grey mould fruit rot of strawberry.
The raw material of microorganism live bacteria preparation that the present invention applies is kluyveromyces marxianus, and the barms can lead to
It crosses commercially available means to be commercially available, the kluyveromyces marxianus used in the present invention is purchased from Shanghai Fu Sheng Industrial Co., Ltd.s.
In order to achieve the above objectives, a kind of microorganism live bacteria preparation is prepared using following steps in the present invention：
1st, kluyveromyces marxianus strain is inoculated into NYDB fluid nutrient mediums, at 28 DEG C, is shaken in 200 r/min
48 h are cultivated in bed;
2nd, cultured saccharomycete bacterium solution under the conditions of 3000 r/min is centrifuged 10 minutes, collects somatic cells, then use
Twice, then washed lower saccharomycete with sterile distilled water and inhaled to beat repeatedly with liquid-transfering gun makes ferment to sterile distilled water washing thalline two or three times
Female bacterium resuspension is counted with blood counting chamber into bacteria suspension, calculates the concentration of yeast bacteria suspension, then adjusted with sterile distilled water
The concentration of bacteria suspension is to 1 × 109 CFU/mL。
Further, the method for application of the microbial bacterial agent is uniformly sprays using the kluyveromyces marxianus
Mist.
Preferably, the microbial bacterial agent of the present invention can be used in compounding with chemical bactericide Boscalid, it is better.
Further, the method for administration of microbial bacterial agent of the invention is that prevention processing is used in premorbid or early stage
Medicine, continuous dispenser three times, are spaced 7-10 days.
Further, when the microbial bacterial agent for using the present invention, crop species, controlling disease depending on application are not
On an equal basis, dosage can change in comparatively wide range.
Compared with prior art, microbial bacterial agent of the invention has the beneficial effect that：
1st, the present invention has widened type of the microorganism formulation for biological control, for the first time by the commercially available known bacterium that can be obtained
Strain finds that it can be used for preventing grey mould fruit rot of strawberry by a large amount of experiment；
2nd, microbial bacterial agent storage properties of the invention are stablized, and control effect is good, the zymotechnique of large-scale production is simple,
Low production cost；
3rd, after microbial bacterial agent of the invention is compounded with Boscalid, the two can perfection coexist, can preferably prevent
Grey mould fruit rot of strawberry；
4th, microbial bacterial agent of the invention is to crop safety, environmentally safe, and efficiency stabilization is more than 45%, with chemistry
Efficiency is more than 85% after fungicide Boscalid compounding, it is possible to reduce the dosage of chemical pesticide.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, present invention specific examples below
It illustrates, but the present invention is limited to absolutely not these examples.As described below is only the preferable embodiment of the present invention, is used only for explaining
The present invention, but cannot be therefore understands that for the limitation to the scope of the claims of the present invention.It should be pointed out that it is all the present invention spirit and
Any modification, equivalent replacement or improvement for being made within principle etc., should all be included in the protection scope of the present invention, therefore,
The protection domain of patent of the present invention should be determined by the appended claims.
Material, reagent used in the embodiment of the present invention etc. unless otherwise instructed, can be bought by commercial sources
It obtains and/or is prepared by known conventional method.
First, the preparation of kluyveromyces marxianus microbial bacterial agent
The preparation method of the kluyveromyces marxianus microbial bacterial agent of the present invention can be prepared via a method which to obtain：
1st, kluyveromyces marxianus strain is inoculated into NYDB fluid nutrient mediums, at 28 DEG C, is shaken in 200r/min
48 h are cultivated in bed;
2nd, cultured saccharomycete bacterium solution under the conditions of 3000 r/min is centrifuged 10 minutes, collects somatic cells, then use
Twice, then washed lower saccharomycete with sterile distilled water and inhaled to beat repeatedly with liquid-transfering gun makes ferment to sterile distilled water exercise thalline two or three times
Female bacterium resuspension is counted with blood counting chamber into bacteria suspension, calculates the concentration of yeast bacteria suspension, then adjusted with sterile distilled water
The concentration of bacteria suspension is to 1 × 109 CFU/mL。
2nd, indoor bioassay
The expection activity that the microbial bacteria suspension of the present invention gives with chemical bactericide Boscalid can be calculated as below（Ginseng
See, Colby, S.R. " Calculating Synergistic and Antagonistic Responses of
Herbicide Combinations”, weed 15, (1967),20-22）:
If
X is the effect when the dosage of x is applied as component A,
Y is the effect when the dosage of y is applied as component B,
E is the effect when component A and component B is applied respectively with the dosage of dosage x and y, then
E=X+Y-XY/100
Effect value is in terms of %, and 0% finger effect is equivalent to control, and effect 100% refers to not observe infection.
If practical Fungicidally active is more than calculated value, the activity of the composition is super adduction, that is, there is collaboration and increase
Effect acts on.In the case that secondary, the effect of actual observation rice has to be larger than the expection effect calculated by formula above-mentioned
（E）.
1st, grey mould fruit rot of strawberry determination of activity is prevented
To the determination of activity of grey mould fruit rot of strawberry using 50% Boscalid water dispersible granules as comparison medicament（Purchased from BASF
Company）, when measuring active protection, by Strawberry seedlings microbial bacterial agent suspension of the present invention and 50% Boscalid water
Dispersible granule is sprayed after being watered dilution, and after hydrojet layer is dried, the aqueous spore suspension of plant grey mould fruit rot of strawberry is inoculated with, it
Afterwards, plant is placed in 20 DEG C with relative humidity to be cultivated in 80% greenhouse.
Inoculation grey mould fruit rot of strawberry spore suspension is evaluated after three days, test group, dosage and result of the test such as table 1
It is shown：
When the microorganism formulation of the present invention is used alone it can be seen from more than result of the test, grey mould fruit rot of strawberry is prevented
It controls and shows certain control effect, when microorganism formulation and the Boscalid effective ingredient consumption 100g/hectare of the present invention
When, control effect can reach 89.7%, show apparent synergistic function, control effect is with 50% Boscalid with effective
200 g a.i./hm of Ingredient Amount2Using when, preventive effect effect is suitable.
Claims (2)
1. a kind of microbial bacterial agent is used to prevent the purposes of grey mould fruit rot of strawberry, which is characterized in that the activity of the microbial bacterial agent
Ingredient is kluyveromyces marxianus bacterium.
2. purposes according to claim 1, which is characterized in that the preparation method of the microbial bacterial agent includes following step
Suddenly：
（1）, kluyveromyces marxianus strain is inoculated into NYDB fluid nutrient mediums, at 28 DEG C, in 200 r/min shaking tables
48 h of middle culture;
（2）, cultured saccharomycete bacterium solution centrifuged 10 minutes under the conditions of 3000 r/min, collect somatic cells, then with nothing
Bacterium distilled water washing thalline twice, is then washed lower saccharomycete and is inhaled repeatedly with liquid-transfering gun and break saccharomycete two or three times with sterile distilled water
Resuspension is counted with blood counting chamber into bacteria suspension, calculates the concentration of yeast bacteria suspension, then with sterile water adjustment bacteria suspension
Concentration is to 1 × 109 CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710185202.1A CN107058141B (en) | 2017-03-25 | 2017-03-25 | A kind of microbial bacterial agent and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710185202.1A CN107058141B (en) | 2017-03-25 | 2017-03-25 | A kind of microbial bacterial agent and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107058141A CN107058141A (en) | 2017-08-18 |
| CN107058141B true CN107058141B (en) | 2018-06-26 |
Family
ID=59618129
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| CN201710185202.1A Active CN107058141B (en) | 2017-03-25 | 2017-03-25 | A kind of microbial bacterial agent and its application |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117737138A (en) * | 2024-02-07 | 2024-03-22 | 云南省林业和草原科学院 | Culture method for inducing Antrodia camphorata with high Li Gongniu to produce bacterial compounds for inhibiting plant diseases |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1813549A (en) * | 2006-03-06 | 2006-08-09 | 江苏大学 | Method for preventing postharvest disease of strawberry |
| CN105543116A (en) * | 2016-02-03 | 2016-05-04 | 北京市农林科学院 | Microzyme strain and application thereof in disease control after fresh fruit picking |
-
2017
- 2017-03-25 CN CN201710185202.1A patent/CN107058141B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1813549A (en) * | 2006-03-06 | 2006-08-09 | 江苏大学 | Method for preventing postharvest disease of strawberry |
| CN105543116A (en) * | 2016-02-03 | 2016-05-04 | 北京市农林科学院 | Microzyme strain and application thereof in disease control after fresh fruit picking |
Non-Patent Citations (2)
| Title |
|---|
| 啶酰菌胺对草莓灰霉病菌的毒力测定及田间防效;杨敬辉等;《江西农业学报》;20100930;94-95 * |
| 马克斯克鲁维酵母对柑橘采后绿霉病的抑制效果;耿鹏等;《华中农业大学学报》;20111231;712-713 * |
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Effective date of registration: 20190920 Address after: 137599 Sijiazi Village, Yongan Town, Tuquan County, Xingan League, Inner Mongolia Autonomous Region Patentee after: Inner Mongolia Yimin Biotechnology Co.,Ltd. Address before: 528000, room 1613, 2, 6 South Fenjiang Road, Chancheng District, Guangdong, Foshan, two Patentee before: FOSHAN RUISHENGTONG TECHNOLOGY Co.,Ltd. |
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Denomination of invention: A Microbial Agent and Its Application Effective date of registration: 20231117 Granted publication date: 20180626 Pledgee: Mengshang Bank Co.,Ltd. Xing'an League Tuquan Branch Pledgor: Inner Mongolia Yimin Biotechnology Co.,Ltd. Registration number: Y2023980066224 |