CN107049863A - A kind of preparation method of plant compound skin-whitening agent nano particle - Google Patents
A kind of preparation method of plant compound skin-whitening agent nano particle Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0216—Solid or semisolid forms
- A61K8/022—Powders; Compacted Powders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The present invention relates to a kind of preparation method of the nano particle of plant compound skin-whitening agent, including by carragheen gellan gum, hydroxypropyl methyl cellulose, Sodium Hyaluronate, disperseed with glycerine, butanediol, water is added thereto, is opened stirring and is heated, is incubated after heating, after be cooled to 50 DEG C, the agent of plant compound skin-whitening, Phenoxyethanol are added thereto, are stirred, are obtained colloidal solution；Afterwards by colloidal solution by high-pressure homogeneous equipment, nanoscale sol solution is obtained；Sol solution is placed in liquid nitrogen afterwards and freezed, nano particle is obtained；The nano particle that this method is obtained, with particle diameter is small, loose structure, is added into cosmetic formulations, with improving the percutaneous absorbability of active material, slow release and reduce irritating effect.The skin irritation of former plant compound skin-whitening agent can be effectively reduced by skin irritation test；Loose structure can improve the slow release of active material, in testing in vitro, can improve inhibitory activity against tyrosinase 18.6%.
Description
Technical field
The present invention relates to a kind of preparation method of plant compound skin-whitening agent, more particularly to a kind of plant compound skin-whitening agent nanometer
The preparation method of particle, belongs to cosmetic technical field.
Background technology
It is beautiful standard that skin and muscles are fair, and everybody has a love of beauty, asian population expect Skin whitening care cosmeticses assign it is fair,
Bright and clean skin, American-European crowd is then mitigated with whitening product even eliminates the pigment depositions such as chloasma, senile plaque expelling.At present, it is domestic
The outer skin-lightening cosmetic market demand increasingly increases, and the research and development of whitening agent deepen continuously, and sequential use is in cosmetics, promotes
The development of beauty and skin care cosmetics.
With the continuous improvement of current people's health consciousness, the idea of rationality whitening turns into main flow, from physical screening
Lid agent (such as iron oxide, titanium dioxide and zinc oxide), the physiological whitening agent controlled to melanocytic system changes, that is, utilizes
Biological agent, Chinese herbal medicine extract etc., exploitation suppress the activity of tyrosinase, prevent melanin from being formed, promote melanin to be metabolized,
With the natural whiting cosmetics for safely and efficiently dispelling skin splash.
And the whitening compound extract of existing traditional Chinese herbal medicine, it there is big taste, color depth, easily separate out and real
The problem of duration is short during the use of border, effect is poor, thus significantly limit its application in skin-lightening cosmetic.
The content of the invention
Using the deficiency existed, there is provided one in skin-lightening cosmetic for existing Chinese herbal medicine whitening compound extract by the present invention
Plant the preparation method of the nano particle of plant compound skin-whitening agent.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) plant compound skin-whitening agent is extracted
Scutellaria, the root bark of white mulberry, oriental wormwood, jujube, radix glycyrrhizae are compared 2 according to weight：1：1：1：1 mixing, carries out water extraction, what is obtained carries
Take liquid to concentrate, after ethanol alcohol precipitation and elution, obtained precipitation is dried in vacuo, plant compound skin-whitening agent is obtained；
2) colloidal solution of plant compound skin-whitening agent is prepared
By carragheen 0.1-0.5 parts by weight, gellan gum 0.1-0.5 parts by weight, hydroxypropyl methyl cellulose 0.1-0.5 weight
Part, Sodium Hyaluronate 0.1-1 parts by weight are disperseed with glycerine 1-5 parts by weight, butanediol 1-5 parts by weight, and water is added thereto
20-50 parts by weight, open stirring and heat, be heated to after 60-90 DEG C being incubated 5-30min, after be cooled to 50 DEG C, add thereto
Plant compound skin-whitening agent 1-3 parts by weight, Phenoxyethanol 0.5-0.8 parts by weight, stir, obtain colloidal solution；
3) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 2) obtained by colloidal solution by high-pressure homogeneous equipment, material is sprayed with the flow velocity of 1000~1500 meter per seconds
Go out, by the void effect, knock-on effect, shearing effect of generation, obtain uniform, fine and smooth, nano level sol solution；
4) porous nano particle is prepared
By step 3) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Further, step 1) in the technological parameter of water extraction be：Solid-liquid ratio 1:15,80 DEG C of temperature, extraction time 4h extracts secondary
Number 3 times.
Further, step 3) described in high-pressure homogeneous equipment feed pressure control in 0.25-0.55MPa, homogenization pressure
Control is in 100-250MPa.
Further, step 1) described in mixing speed be 50-200r/min.
The beneficial effects of the invention are as follows：
Compound skin-whitening agent is wrapped up by high pressure freezing for this method and refinement obtains nano particle, makes its particle diameter
It is small, and be uniformly distributed in loose structure, it is added into cosmetic formulations, the percutaneous absorbability with raising active material,
Slow release and the irritating effect of reduction.The skin of former plant compound skin-whitening agent can be effectively reduced by skin irritation test
Excitant；Loose structure can improve the slow release of active material, in testing in vitro, can improve inhibitory activity against tyrosinase
18.6%.
Embodiment
The principle and feature of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
Plant compound skin-whitening agent used in the present invention is according to weight ratio by scutellaria, the root bark of white mulberry, oriental wormwood, jujube, radix glycyrrhizae
2：1：1：1：What the mixture water extraction of 1 mixing was obtained, extraction process is solid-liquid ratio 1:15th, 80 DEG C of temperature, extraction time 4h, extraction
Number of times 3 times.Obtained extract solution concentration, respectively through 60% ethanol alcohol precipitation and 80% ethanol alcohol precipitation, and with anhydrous ethanol elution 2
It is secondary to be finally dried in vacuo obtained precipitation with removing impurity therein, obtain plant compound skin-whitening agent solid.
Embodiment 1：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.1, the parts by weight of gellan gum 0.1, the parts by weight of hydroxypropyl methyl cellulose 0.1, hyaluronic acid
The parts by weight of sodium 0.1 are disperseed with the parts by weight of glycerine 1, the parts by weight of butanediol 1, and the parts by weight of water 20 are added thereto, open stirring
And heating, mixing speed is 100r/min, and 20min is incubated after being heated to 60 DEG C, after be cooled to 50 DEG C, thereto add plant answer
The square parts by weight of whitening agent 1, the parts by weight of Phenoxyethanol 0.5, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.25-0.35MPa,
Matter Stress control is sprayed with the flow velocity of 1000~1200 meter per seconds in 100-120MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Embodiment 2：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.5, the parts by weight of gellan gum 0.4, the parts by weight of hydroxypropyl methyl cellulose 0.2, hyaluronic acid
The parts by weight of sodium 0.2 are disperseed with the parts by weight of glycerine 5, the parts by weight of butanediol 1, and the parts by weight of water 35 are added thereto, open stirring
And heating, mixing speed is 150r/min, and 15min is incubated after being heated to 70 DEG C, after be cooled to 50 DEG C, thereto add plant answer
The square parts by weight of whitening agent 3, the parts by weight of Phenoxyethanol 0.8, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.35-0.45MPa,
Matter Stress control is sprayed with the flow velocity of 1200~1300 meter per seconds in 150-180MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Embodiment 3：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.5, the parts by weight of gellan gum 0.5, the parts by weight of hydroxypropyl methyl cellulose 0.5, hyaluronic acid
The parts by weight of sodium 1 are disperseed with the parts by weight of glycerine 5, the parts by weight of butanediol 5, thereto add the parts by weight of water 50, open stirring and
Heating, mixing speed is 50r/min, and 5min is incubated after being heated to 90 DEG C, after be cooled to 50 DEG C, plant compound is added thereto beautiful
The white parts by weight of agent 3, the parts by weight of Phenoxyethanol 0.8, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.45-0.55MPa,
Matter Stress control is sprayed with the flow velocity of 1300~1500 meter per seconds in 220-250MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；
3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Embodiment 4：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.1, the parts by weight of gellan gum 0.1, the parts by weight of hydroxypropyl methyl cellulose 0.1, hyaluronic acid
The parts by weight of sodium 0.1 are disperseed with the parts by weight of glycerine 5, the parts by weight of butanediol 5, and the parts by weight of water 35 are added thereto, open stirring
And heating, mixing speed is 100r/min, and 30min is incubated after being heated to 60 DEG C, after be cooled to 50 DEG C, thereto add plant answer
The square parts by weight of whitening agent 3, the parts by weight of Phenoxyethanol 0.5, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.25-0.35MPa,
Matter Stress control is sprayed with the flow velocity of 1000~1200 meter per seconds in 100-120MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；
3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Embodiment 5：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.5, the parts by weight of gellan gum 0.5, the parts by weight of hydroxypropyl methyl cellulose 0.5, hyaluronic acid
The parts by weight of sodium 1 are disperseed with the parts by weight of glycerine 1, the parts by weight of butanediol 1, thereto add the parts by weight of water 20, open stirring and
Heating, mixing speed is 200r/min, and 15min is incubated after being heated to 75 DEG C, after be cooled to 50 DEG C, thereto add plant compound
The parts by weight of whitening agent 1, the parts by weight of Phenoxyethanol 0.8, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.35-0.45MPa,
Matter Stress control is sprayed with the flow velocity of 1200~1300 meter per seconds in 150-180MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；
3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Embodiment 6：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.5, the parts by weight of gellan gum 0.4, the parts by weight of hydroxypropyl methyl cellulose 0.2, hyaluronic acid
The parts by weight of sodium 0.2 are disperseed with the parts by weight of glycerine 5, the parts by weight of butanediol 5, and the parts by weight of water 50 are added thereto, open stirring
And heating, mixing speed is 50r/min, and 20min is incubated after being heated to 90 DEG C, after be cooled to 50 DEG C, thereto add plant answer
The square parts by weight of whitening agent 1, the parts by weight of Phenoxyethanol 0.8, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.35-0.45MPa,
Matter Stress control is sprayed with the flow velocity of 1300~1500 meter per seconds in 180-200MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；
3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Embodiment 7：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.1, the parts by weight of gellan gum 0.1, the parts by weight of hydroxypropyl methyl cellulose 0.1, hyaluronic acid
The parts by weight of sodium 0.1 are disperseed with the parts by weight of glycerine 1, the parts by weight of butanediol 5, and the parts by weight of water 50 are added thereto, open stirring
And heating, mixing speed is 150r/min, and 15min is incubated after being heated to 75 DEG C, after be cooled to 50 DEG C, thereto add plant answer
The square parts by weight of whitening agent 3, the parts by weight of Phenoxyethanol 0.5, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.45-0.55MPa,
Matter Stress control is sprayed with the flow velocity of 1400~1500 meter per seconds in 220-250MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；
3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
Embodiment 8：
A kind of preparation method of the nano particle of plant compound skin-whitening agent, comprises the following steps：
1) colloidal solution of plant compound skin-whitening agent is prepared
By the parts by weight of carragheen 0.5, the parts by weight of gellan gum 0.3, the parts by weight of hydroxypropyl methyl cellulose 0.3, hyaluronic acid
The parts by weight of sodium 0.2 are disperseed with the parts by weight of glycerine 1, the parts by weight of butanediol 1, and the parts by weight of water 20 are added thereto, open stirring
And heating, mixing speed is 200r/min, and 5min is incubated after being heated to 90 DEG C, after be cooled to 50 DEG C, thereto add plant answer
The square parts by weight of whitening agent 1, the parts by weight of Phenoxyethanol 0.8, stir, obtain colloidal solution；
2) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 1) obtained by colloidal solution by high-pressure homogeneous equipment, feed pressure control is in 0.25-0.35MPa,
Matter Stress control is sprayed with the flow velocity of 1000~1200 meter per seconds in 100-120MPa, material, by the void effect of generation, hit
Effect, shearing effect are hit, uniform, fine and smooth, nano level sol solution is obtained；
3) porous nano particle is prepared
By step 2) obtained by sol solution be placed in liquid nitrogen and freeze, after being transferred to after product fully charge in refrigerator in -18
Preserved in environment below DEG C.
In order to verify the technique effect for the nano-solid that the preparation method of the present invention is obtained, We conducted following test：
First, security test
1.1 test material
Spot tries device：The production of Beijing North doctor's management of investment Co., Ltd, circular, diameter 12mm.
Spot tries thing：The ascorbic acid four of 5wt% Ascorbyl Tetraisopalmitate solution, the 5wt% gained of embodiment 2
Different palmitate nano-solid solution.
1.2 Subject Populations and testing time
Effective subject amounts to 30 people, and specific sex composition and age composition determine (to meet 2007 at random《Cosmetics health
Specification》Include, exclusion standard.
1.3 experimental method
Subject is selected by subject enrollment standard, number is 30.From qualified patch material, sample is put into spot
Try in device (amount of samples is 0.020g-0.025g), control wells are blank (not putting any material).Sample and blank control is equal
The bent side of forearm of subject is affixed on, is gently pressed with palm and is allowed to equably be pasted on skin, continue 24h.Between removing after patch device
Every 30min, dermoreaction is observed after impression disappears.24h and 48h are observed once again respectively after patch test, and observation skin is anti-
Should.Skin adverse reaction grade scale is shown in Table 1：
Table 1：Skin adverse reaction is classified
1.4 results are explained：
Skin closure type patch test results are explained：The number for occurring 1 grade of skin adverse reaction in 30 subjects is more than 5
Example, or the number of 2 grades of skin adverse reactions are more than 2, or when there is any 13 grades or more than 3 grades skin adverse reactions, sentence
Determine tested material has adverse reaction to human body.
1.5 experimental result
Result of the test is shown in Table 2,3,4.
Table 2 removes 0.5 hour observation result of spot examination device
Table 3 removes 24 hours observation results of spot examination device
Table 4 removes 48 hours observation results of spot examination device
Test result indicates that：This patch test results, according to 2007《Cosmetics health specification》Requirement, judge
5wt% Ascorbyl Tetraisopalmitate solution has adverse reaction, the 5wt% different palmitic acid of ascorbic acid four to human body skin
Ester nano-solid solution has no adverse reaction to human body.
2nd, tyrosinase inhibition rate is tested
2.1 instrument：Spectrophotometer, micropipettor (1mL), electronic balance, volumetric flask (10mL, 50mL, 250mL),
Constant temperature water box, beaker and graduated cylinder are some.
2.2 sample：Potato, TYR, sodium dihydrogen phosphate, disodium hydrogen phosphate, deionized water
2.3 preparation of reagents：
Phosphate buffer solution (pH=6.8)：0.2mol/L sodium dihydrogen phosphate and 0.2mol/L phosphorus are first prepared respectively
Sour disodium hydrogen.
0.2mol/L sodium dihydrogen phosphates：Weigh 71.6g Na2HPO4-12H2O, is dissolved in 1000mL deionized waters；
0.2mol/L disodium hydrogen phosphates：Weigh 31.2g NaH2PO4-2H2O, is dissolved in 1000mL deionized waters；Take 51mL phosphorus
Acid dihydride sodium+49mL disodium hydrogen phosphates produce 0.2mo l/L, pH=6.8 phosphate buffer.
TYR solution：TYR 25.6g is weighed, with phosphate buffer constant volume in 50mL volumetric flasks, L- is produced
Tyrosine solution.
Tyrosinase solution：Potato is cleaned, in 4 DEG C of precooling 4h or so.Peeling, is cut into about 1.0cm3Fourth shape, in -20
DEG C freeze overnight.Weigh, by 1：1(W:V ratio) adds the sodium phosphate buffer of 4 DEG C of precoolings, is made of tissue mashing machine even
Slurry, 3 layers of filtered through gauze, filtrate centrifuges 10min in 4000r/min, and supernatant is the tyrosinase crude enzyme liquid of gained, 4 DEG C of guarantors
Deposit, be finished in 2h.
Plant compound skin-whitening agent is prepared by test solution：Plant compound skin-whitening agent is made into 5mg/mL solution and dilute with methanol
Release 1mg/mL.
Plant compound skin-whitening agent nano particle is prepared by test solution：By the plant compound skin-whitening agent nano particle of embodiment 3
It is made into 5mg/mL solution and with methanol dilution to 1mg/mL.
2.4 experimental method
According to phosphate buffer solution, sample solution, tyrosine solution is sequentially added into test tube table 5 Suo Shi, in 35 DEG C
Water-bath 10 minutes.Then tyrosine enzyme liquid is added, is mixed, then 30min is incubated at 35 DEG C, is transferred quickly in cuvette,
Absorbance is determined at 475nm.
Table 5：The composition of prepare liquid
Using each group absorbance, by calculating tyrosinase inhibition rate, its calculation formula is as follows:
A:The absorbance A (A) that the enzyme-added mixed liquor of product is surveyed is not loaded；
B:The product absorbance A (B) that also not enzyme-added mixed liquor is surveyed is not loaded；
C:The absorbance A (C) that the mixed liquor of sample-adding product and enzyme is surveyed；
D:Sample-adding product and absorbance A (D) that not enzyme-added mixed liquor is surveyed.
Using each group absorbance, by calculating tyrosinase inhibition rate, its calculation formula is as follows:
2.5 experimental result
By above-mentioned detection method, 2 kinds are detected by test solution, the inhibiting rate of the two is drawn, is specifically shown in Table 6：
Table 6：Two kinds of inhibiting rates by test solution compare
Test result indicates that：Energy of the plant compound skin-whitening agent nano particle compared with the suppression tyrosinase of plant compound skin-whitening agent
Power is significantly increased.Tyrosinase inhibition rate is higher, and the melanin generated in skin is fewer, and whitening effect will be better, this
As a result the active matter prepared through high pressure freezing is further illustrated, whitening performance is increased dramatically.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (4)
1. a kind of preparation method of plant compound skin-whitening agent nano particle, it is characterised in that comprise the following steps：
1) plant compound skin-whitening agent is extracted
Scutellaria, the root bark of white mulberry, oriental wormwood, jujube, radix glycyrrhizae are compared 2 according to weight：1：1：1：1 mixing, carries out water extraction, obtained extract solution
Concentration, after ethanol alcohol precipitation and elution, obtained precipitation is dried in vacuo, plant compound skin-whitening agent is obtained；
2) colloidal solution of plant compound skin-whitening agent is prepared
By carragheen 0.1-0.5 parts by weight, gellan gum 0.1-0.5 parts by weight, hydroxypropyl methyl cellulose 0.1-0.5 parts by weight,
Sodium Hyaluronate 0.1-1 parts by weight are disperseed with glycerine 1-5 parts by weight, butanediol 1-5 parts by weight, and water 20- is added thereto
50 parts by weight, open stirring and heat, be heated to after 60-90 DEG C being incubated 5-30min, after be cooled to 50 DEG C, thereto add plant
Thing compound skin-whitening agent 1-3 parts by weight, Phenoxyethanol 0.5-0.8 parts by weight, stir, obtain colloidal solution；
3) the nanometer sol solution of plant compound skin-whitening agent is prepared
By step 2) obtained by colloidal solution by high-pressure homogeneous equipment, material is sprayed with the flow velocity of 1000~1500 meter per seconds,
By the void effect, knock-on effect, shearing effect of generation, uniform, fine and smooth, nano level sol solution is obtained；
4) porous nano particle is prepared
By step 3) obtained by sol solution be placed in liquid nitrogen freeze, after be transferred to after product fully charge in refrigerator in -18 DEG C with
Under environment in preserve.
2. preparation method according to claim 1, it is characterised in that step 1) in the technological parameter of water extraction be：Solid-liquid ratio
1:15,80 DEG C of temperature, extraction time 4h, extraction time 3 times.
3. preparation method according to claim 1, it is characterised in that step 3) the feed pressure control of mesohigh homogenizer
System is controlled in 100-250MPa in 0.25-0.55MPa, homogenization pressure.
4. the preparation method according to any one of claim 1-3, it is characterised in that step 1) described in mixing speed
For 50-200r/min.
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| Publication number | Priority date | Publication date | Assignee | Title |
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