CN107043707A - A kind of selenium-enriched cordceps militaris inoculation method - Google Patents
A kind of selenium-enriched cordceps militaris inoculation method Download PDFInfo
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- CN107043707A CN107043707A CN201611075838.2A CN201611075838A CN107043707A CN 107043707 A CN107043707 A CN 107043707A CN 201611075838 A CN201611075838 A CN 201611075838A CN 107043707 A CN107043707 A CN 107043707A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention belongs to cordyceps sinensis inoculation method technical field, and in particular to a kind of selenium-enriched cordceps militaris inoculation method.A kind of selenium-enriched cordceps militaris inoculation method of the present invention, conventionally by solid medium culture and fluid nutrient medium culture, the compound method of described fluid nutrient medium is：Take the composition of following portions by weight：Potassium dihydrogen phosphate 0.03 0.06, magnesium sulfate 0.03 0.05, ammonium citrate 8 10, glucose 15 25, peptone 68 and vitamin B1 0.01 0.02 are put into selenium-rich water 900 1000, the autoclaving 30 minutes under the conditions of temperature is 120-125 °.The present invention adds selenium-rich raw material in the culture medium of Cordyceps militaris so that Se content increase in the cordyceps sinensis of cultivation, further improves the value of Cordyceps militaris.
Description
Technical field
The invention belongs to cordyceps sinensis inoculation method technical field, and in particular to a kind of selenium-enriched cordceps militaris inoculation method.
Background technology
The nutritive value and medical value of Cordyceps militaris are constantly discovered in recent years, the cordycepin in Cordyceps militaris, can it is antiviral,
Antibacterial, the tumour growth that substantially suppresses, interference human body RNA and DNA synthesis；Riched Cordyceps Militaris contains cordycepic acid, and cordycepic acid can effectively prevent
Cerebral thrombus, cerebral hemorrhage, renal failure are treated, while also having diuresis；Adenosine in Cordyceps militaris, can be with antiviral, anti-
Bacterium, suppresses Platelet congregation preventing and treating thrombosis, can also eliminate face spot, anti-ageing wrinkle resistant；Cordyceps militaris is also rich in selenium, substantial amounts of science
It facts have proved that selenium can significantly suppress the growth of cancer cell, stimulate the generation of immunoglobulin and antibody, strengthen immunity of organism
With oxidation resistance etc., therefore it is most important to the value for improving Cordyceps militaris to improve the Se content in Cordyceps militaris.
The content of the invention
It is an object of the invention to provide a kind of selenium-enriched cordceps militaris inoculation method.
The technical scheme is that：
A kind of selenium-enriched cordceps militaris inoculation method, is conventionally trained by solid medium culture and fluid nutrient medium
Support, the compound method of described fluid nutrient medium is：Take the composition of following portions by weight：Potassium dihydrogen phosphate 0.03-0.06, sulfuric acid
Magnesium 0.03-0.05, ammonium citrate 8-10, glucose 15-25, peptone 6-8 and vitamin B1 0.01-0.02 are put into selenium-rich water
In 900-1000, the autoclaving 30 minutes under the conditions of temperature is 120-125 °；
Described selenium-rich water is the one or two kinds of any of the above ratio in selenium-rich potato, selenium-rich rice and selenium-rich corn
Mixture add according to every 100 grams of solids and boiled in the ratio boiling water of 1 liter of water 1-2 hours, take filtrate cooling to be made after filtering.
The present invention uses above-mentioned technical proposal, and selenium-rich raw material is added in the culture medium of Cordyceps militaris so that the worm of cultivation
Se content increase in grass, further improves the value of Cordyceps militaris.
Embodiment
Embodiment 1
A kind of selenium-enriched cordceps militaris inoculation method, is conventionally trained by solid medium culture and fluid nutrient medium
Support, the compound method of described fluid nutrient medium is：Take the composition of following portions by weight：Potassium dihydrogen phosphate 0.03, magnesium sulfate
0.03rd, ammonium citrate 8, glucose 15, peptone 6 and vitamin B1 0.01 are put into selenium-rich water 900, are 120 ° of bars in temperature
Autoclaving 30 minutes under part；
Described selenium-rich water adds for selenium-rich potato according to every 100 grams of selenium-rich potatoes boils 1 hour in the ratio boiling water of 1 liter of water,
Filtrate cooling is taken to be made after filtering.
Embodiment 2
A kind of selenium-enriched cordceps militaris inoculation method, is conventionally trained by solid medium culture and fluid nutrient medium
Support, the compound method of described fluid nutrient medium is：Take the composition of following portions by weight：Potassium dihydrogen phosphate 0.06, magnesium sulfate
0.05th, ammonium citrate 10, glucose 25, peptone 8 and vitamin B1 0.02 are put into selenium-rich water 1000, are 125 ° in temperature
Under the conditions of autoclaving 30 minutes；
Described selenium-rich water adds the ratio of 1 liter of water for the mixture of selenium-rich potato and selenium-rich rice according to every 100 grams of solids
Boiled in boiling water 2 hours, take filtrate cooling to be made after filtering.
Embodiment 3
A kind of selenium-enriched cordceps militaris inoculation method, is conventionally trained by solid medium culture and fluid nutrient medium
Support, the compound method of described fluid nutrient medium is：Take the composition of following portions by weight：Potassium dihydrogen phosphate 0.04, magnesium sulfate
0.04th, ammonium citrate 9, glucose 20, peptone 7 and vitamin B1 0.01 are put into selenium-rich water 950, are 121 ° of bars in temperature
Autoclaving 30 minutes under part；
Described selenium-rich water adds the ratio of 1 liter of water for the mixture in selenium-rich rice and selenium-rich corn according to every 100 grams of solids
Boiled 1.5 hours in example boiling water, take filtrate cooling to be made after filtering.
Claims (2)
1. a kind of selenium-enriched cordceps militaris inoculation method, conventionally by solid medium culture and fluid nutrient medium culture
Two, it is characterized in that the compound method of described fluid nutrient medium is：Take the composition of following portions by weight：Potassium dihydrogen phosphate 0.03-
0.06th, magnesium sulfate 0.03-0.05, ammonium citrate 8-10, glucose 15-25, peptone 6-8 and vitaminB10 .01-0.02 are put
Enter in selenium-rich water 900-1000, the autoclaving 30 minutes under the conditions of temperature is 120-125 °.
2. selenium-enriched cordceps militaris inoculation method according to claim 1, it is characterized in that described selenium-rich water is selenium-rich potato, richness
The mixture of one or two kinds of any of the above ratio in selenium rice and selenium-rich corn adds 1 liter of water according to every 100 grams of solids
Boiled in ratio boiling water 1-2 hours, take filtrate cooling to be made after filtering.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611075838.2A CN107043707A (en) | 2016-11-30 | 2016-11-30 | A kind of selenium-enriched cordceps militaris inoculation method |
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| CN201611075838.2A CN107043707A (en) | 2016-11-30 | 2016-11-30 | A kind of selenium-enriched cordceps militaris inoculation method |
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| CN107043707A true CN107043707A (en) | 2017-08-15 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220536A (en) * | 2018-11-20 | 2019-01-18 | 常德炎帝牧源农业发展有限公司 | A kind of inoculation method of organic selenium-rich Cordyceps militaris |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998037172A1 (en) * | 1997-02-21 | 1998-08-27 | Viva America Marketing, Inc. | Dietary supplementation with, and methods for preparation of yeast-derived selenium salts |
| CN101898908A (en) * | 2009-05-26 | 2010-12-01 | 湖北圣峰药业有限公司 | Culture medium formula for producing biological selenium-enriched cordceps militaris |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
-
2016
- 2016-11-30 CN CN201611075838.2A patent/CN107043707A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998037172A1 (en) * | 1997-02-21 | 1998-08-27 | Viva America Marketing, Inc. | Dietary supplementation with, and methods for preparation of yeast-derived selenium salts |
| CN101898908A (en) * | 2009-05-26 | 2010-12-01 | 湖北圣峰药业有限公司 | Culture medium formula for producing biological selenium-enriched cordceps militaris |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
Non-Patent Citations (4)
| Title |
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| 于田田等: "生物富硒对蛹虫草菌丝体化学成分的影响", 《食品科技》 * |
| 周毅峰等: "硒对蛹虫草菌丝代谢和总抗氧化能力的影响", 《中国酿造》 * |
| 袁泰斗: "影响蛹虫草子实体产量及质量的因素研究", 《广东农业科学》 * |
| 钟鸣等: "蛹虫草中硒的赋存形态及蛋白硒分析", 《广东微量元素科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220536A (en) * | 2018-11-20 | 2019-01-18 | 常德炎帝牧源农业发展有限公司 | A kind of inoculation method of organic selenium-rich Cordyceps militaris |
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Application publication date: 20170815 |