CN101898908A - Culture medium formula for producing biological selenium-enriched cordceps militaris - Google Patents
Culture medium formula for producing biological selenium-enriched cordceps militaris Download PDFInfo
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- CN101898908A CN101898908A CN2009100622121A CN200910062212A CN101898908A CN 101898908 A CN101898908 A CN 101898908A CN 2009100622121 A CN2009100622121 A CN 2009100622121A CN 200910062212 A CN200910062212 A CN 200910062212A CN 101898908 A CN101898908 A CN 101898908A
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- cordceps militaris
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Abstract
The invention relates to a growth matrix formula used in a fungus cultivation method, in particular to a culture medium formula for producing biological selenium-enriched cordceps militaris, and provides a new growth matrix formula for producing the biological selenium-enriched cordceps militaris by the existing production method. The growth matrix formula comprises the following components based on the packed weight of 500ml of a culture vessel: 0.3g-2.0g of glucose, 0.03g-0.1g of KH2PO4, 0.01g-0.05g of magnesium sulphate, 12mg-8mg of vitamin B, 40g-120g of living silkworm chrysalis and 29.5ug-700ug of selenium. Compared with the prior art, the culture medium formula has the characteristics of simple operation, good stability, and being capable of manually controlling selenium content in a biological selenium-enriched cordyceps fruiting body cultured by the process of the invention.
Description
Technical field
The present invention relates to the growth matrix prescription that uses in the fungi cultivation method.Be particularly useful for producing the culture medium prescription of biological selenium-enriched cordceps militaris.
Technical background
Cordyceps militaris (L.) Link. has another name called Cordyceps militaris (L.) Link., and higher edibleness is not only arranged, and higher pharmaceutical use is arranged.Contain cordycepin, cordycepic acid, Cordyceps polysaccharide superoxide-dismutase, Nucleotide, microorganism etc. in the Cordyccps-militaris-(L.)-link. Sporophore, have effects such as calmness, antifatigue, antitumor, anti-ageing, anticancer.
Selenium-enriched cordceps militaris be to the north of the cordyceps species Cordyceps militaris (L.) Link. goods cultivating, process through the rich seleno culture medium of artificial organ and bio-transformation condition, experimental study shows, selenium-enriched cordceps militaris is except that chemistry industry composition with natural cs and pharmacological action, also contain abundant selenic ester polysaccharide, selenium-Dai methionine(Met), selenium-organic selenium compounds such as Dai Gelucystine, its anti-ageing, antitumor and strong nourishing are better than natural Cordyceps militaris (L.) Link. and other cultivation Cordyccps-militaris-(L.)-link. Sporophore.
Summary of the invention:
The purpose of this invention is to provide a kind of new growth matrix prescription,, can produce biological selenium-enriched cordceps militaris with existing production method.
The present invention realizes with following technical scheme; Produce the culture medium prescription of biological selenium-enriched cordceps militaris, contain in the 500ml flask culture base: glucose 0.3g-2.0g, KH
2PO
40.03g～0.1g, sal epsom 0.01g-0.05g, vitamins B
12mg～8mg, live body grasshopper silkworm chrysalis 40g-120g, selenium 29.5ug-700ug.
The selenium content that the present invention produces the culture medium prescription of biological selenium-enriched cordceps militaris is to use concentrated rich selenium spring to add.
Receive by glucose, biphosphate by containing for the culture medium prescription of producing biological selenium-enriched cordceps militaris, sal epsom, vitamins B
1, live body grasshopper silkworm chrysalis, to concentrate mineral water rich in selenium be that preparation of raw material forms.Its compound method is to get other raw material that removes live body grasshopper silkworm chrysalis in the prescription to mix, and adds an amount of rich selenium spring and the distilled water thermosol of concentrating, sterilization, and standby with a small amount of distilled water constant volume as nutritive medium.Get live body grasshopper silkworm chrysalis after the sterilization in culturing bottle, launch, evenly add above-mentioned nutritive medium, be the selenium-enriched cordceps militaris substratum.
The selenium content that the present invention has in the biological selenium worm grass sporophore that this technology cultivates compared with the prior art can Artificial Control, and stable good characteristics simple to operate.
There were significant differences for the culture medium prescription of production biological selenium worm grass of the present invention and literature method.On prescription, make full use of the local resource that enriches of Hubei Province's Enshitujianationalitymiaonationality Autonomous Prefecture, concentrated mineral water rich in selenium, the organic transformation rate height of selenium.The cultivation prescription of production selenium-enriched cordceps militaris of the present invention can be according to the organic selenium content standard of customer need, the selenium-enriched cordceps militaris of supply selenium content 5～110mg/kg.
Use the substratum production selenium-enriched cordceps militaris technological process of production of production biological selenium worm grass of the present invention as follows
1, the preparation of substratum: get glucose 10g～20g earlier, KH
2PO
40.5g～1g, sal epsom 0.1g～0.5g, vitamins B
10.05g be dissolved in 1000 milliliters the tap water, add an amount of selenium mineral water that concentrates, sterilization, and make nutrient solution with the distilled water constant volume, standby, select for use again the culturing bottle of diameter 10cm will be the live body grasshopper silkworm chrysalis of sterilization in culture bottle, launch, evenly add above-mentioned nutritive medium, bottleneck is tight with polypropylene foil film envelope.
2, inoculation: anti-selenium power is strong, and the high high-quality liquid spawn of selenium transformation efficiency in the ratio of every bottle of 2～10ml, is evenly injected the pupal cell surface under aseptic cleaning condition, simultaneously with the bottleneck envelope tightly.
3, the culturing bottle with inoculation moves in the dark place, and 10～22 ℃ of controlled temperature treat that mycelia infects polypide, and mycelia was infected polypide fully in general 12～20 days.
4, treat that mycelia is infected polypide fully after, gaining in strength is the careless and sloppy rayed of 100～1000 luxs, is no less than 14 hours every day, mycelia transfers orange to by white after 3～5 days, 15～25 ℃ of controlled temperature are treated the sporophore germination and growth.
5, after the cultivation in about 50～70 days, the sporophore maturation is gathered.
Domestic data shows, study the few of the rich selenium of Cordyceps militaris (L.) Link., and the natural cs selenium content is extremely low, only between 0.22～0.96mg/kg, the culture medium prescription of production selenium-enriched cordceps militaris of the present invention and cultivation processing condition, can be according to the organic selenium content standard of customer need, for the selenium-enriched cordceps militaris that contains selenium 5～110mg/kg.
Embodiment
The present invention is further illustrated below in conjunction with embodiment:
It is that the culture medium prescription of 5ug/g selenium-enriched cordceps militaris sporophore is that embodiment 1 the present invention produces selenium content: by 500ml culture vessel Intake Quantity: contain glucose 1g, KH
2PO
40.08g, vitamins B
14mg, MgSO
40.05g, live body grasshopper silkworm chrysalis 70g, getting other raw material that removes live body grasshopper silkworm chrysalis in the prescription mixes, add an amount of mineral water rich in selenium and tap water thermosol, and it is standby as nutritive medium to be settled to 10ml with a small amount of distilled water, regulates addition according to the selenium concentration of selenium mineral water simultaneously, making every bottle, to contain the selenium total amount be 29.5ug, live body grasshopper silkworm chrysalis after will sterilizing then launches in culturing bottle, evenly adds above-mentioned nutritive medium, finishes culture medium preparation.Cultivate Cordyccps-militaris-(L.)-link. Sporophore by above-mentioned technical process, can get the selenium-enriched cordceps militaris sporophore of selenium content 5ug/g.
It is that the culture medium prescription of 20ug/g selenium-enriched cordceps militaris sporophore is that embodiment 2 the present invention produce selenium content: by 500ml culture vessel Intake Quantity: contain glucose 1g, KH
2PO
40.08g, vitamins B
14mg, MgSO
40.05g, live body grasshopper silkworm chrysalis 70g, getting other raw material that removes live body grasshopper silkworm chrysalis in the prescription mixes, add an amount of mineral water rich in selenium and tap water thermosol, and it is standby as nutritive medium to be settled to 10ml with a small amount of distilled water, regulates addition according to the selenium concentration of selenium mineral water simultaneously, making every bottle, to contain the selenium total amount be 116.5ug, live body grasshopper silkworm chrysalis after will sterilizing then launches in culturing bottle, evenly adds above-mentioned nutritive medium, finishes culture medium preparation.Cultivate Cordyccps-militaris-(L.)-link. Sporophore by above-mentioned technical process, can get the selenium-enriched cordceps militaris sporophore of selenium content 20ug/g.
It is that the culture medium prescription of 40ug/g selenium-enriched cordceps militaris sporophore is that embodiment 3 the present invention produce selenium content: by 500ml culture vessel Intake Quantity: contain glucose 2g, KH
2PO
40.1g, vitamins B
14mg, MgSO
40.05g, live body grasshopper silkworm chrysalis 70g, getting other raw material that removes live body grasshopper silkworm chrysalis in the prescription mixes, add an amount of mineral water rich in selenium and tap water thermosol, and it is standby as nutritive medium to be settled to 10ml with a small amount of distilled water, regulates addition according to the selenium concentration of selenium mineral water simultaneously, making every bottle, to contain the selenium total amount be 233ug, live body grasshopper silkworm chrysalis after will sterilizing then launches in culturing bottle, evenly adds above-mentioned nutritive medium, finishes culture medium preparation.Cultivate Cordyccps-militaris-(L.)-link. Sporophore by above-mentioned technical process, can get the selenium-enriched cordceps militaris sporophore of selenium content 40ug/g.
It is that the culture medium prescription of 80ug/g selenium-enriched cordceps militaris sporophore is that embodiment 4 the present invention produce selenium content: by 500ml culture vessel Intake Quantity: contain glucose 2g, KH
2PO
40.1g, vitamins B
15mg, MgSO
40.05g, live body grasshopper silkworm chrysalis 70g, getting other raw material that removes live body grasshopper silkworm chrysalis in the prescription mixes, add an amount of mineral water rich in selenium and tap water thermosol, and it is standby as nutritive medium to be settled to 10ml with a small amount of distilled water, regulates addition according to the selenium concentration of selenium mineral water simultaneously, making every bottle, to contain the selenium total amount be 466.5ug, live body grasshopper silkworm chrysalis after will sterilizing then launches in culturing bottle, evenly adds above-mentioned nutritive medium, finishes culture medium preparation.Cultivate Cordyccps-militaris-(L.)-link. Sporophore by above-mentioned technical process, can get the selenium-enriched cordceps militaris sporophore of selenium content 80ug/g.
It is that the culture medium prescription of 110ug/g selenium-enriched cordceps militaris sporophore is that embodiment 5 the present invention produce selenium content: by 500ml culture vessel Intake Quantity: contain glucose 2g, KH
2PO
40.1g, vitamins B
15mg, MgSO
40.05g, live body grasshopper silkworm chrysalis 70g, getting other raw material that removes live body grasshopper silkworm chrysalis in the prescription mixes, add an amount of mineral water rich in selenium and tap water thermosol, and it is standby as nutritive medium to be settled to 10ml with a small amount of distilled water, regulates addition according to the selenium concentration of selenium mineral water simultaneously, making every bottle, to contain the selenium total amount be 700ug, live body grasshopper silkworm chrysalis after will sterilizing then launches in culturing bottle, evenly adds above-mentioned nutritive medium, finishes culture medium preparation.Cultivate Cordyccps-militaris-(L.)-link. Sporophore by above-mentioned technical process, can get the selenium-enriched cordceps militaris sporophore of selenium content 110ug/g.
Claims (2)
1. a culture medium prescription of producing biological selenium-enriched cordceps militaris is characterized in that containing in the 500ml flask culture base: glucose 0.3g-2.0g, KH
2PO
40.03g～0.1g, sal epsom 0.01g-0.05g, vitamins B
12mg～8mg, live body grasshopper silkworm chrysalis 40g-120g, selenium 29.5ug-700ug.
2. want 1 described a kind of culture medium prescription of producing biological selenium-enriched cordceps militaris according to right, it is characterized in that described selenium content is to use concentrated rich selenium spring to add.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100622121A CN101898908A (en) | 2009-05-26 | 2009-05-26 | Culture medium formula for producing biological selenium-enriched cordceps militaris |
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|---|---|---|---|
| CN2009100622121A CN101898908A (en) | 2009-05-26 | 2009-05-26 | Culture medium formula for producing biological selenium-enriched cordceps militaris |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138436A (en) * | 2010-12-28 | 2011-08-03 | 湖北圣峰药业有限公司 | Method for culturing selenium-enriched Cordyceps militaris |
| CN103030452A (en) * | 2013-01-17 | 2013-04-10 | 李卫旗 | Method for preparing liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris |
| CN104770660A (en) * | 2014-01-13 | 2015-07-15 | 于汇 | Cordyceps sinensis rice with rich cordyceps militaris nutrients and rich medicinal values, and production process thereof |
| CN106957190A (en) * | 2017-04-24 | 2017-07-18 | 广西环江仙草农舍生物科技有限公司 | Cordyceps militaris solid medium |
| CN106978300A (en) * | 2017-04-24 | 2017-07-25 | 广西环江仙草农舍生物科技有限公司 | The preparation method of cordyceps wine |
| CN107043707A (en) * | 2016-11-30 | 2017-08-15 | 广州市瑞硒康生物科技有限公司 | A kind of selenium-enriched cordceps militaris inoculation method |
-
2009
- 2009-05-26 CN CN2009100622121A patent/CN101898908A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138436A (en) * | 2010-12-28 | 2011-08-03 | 湖北圣峰药业有限公司 | Method for culturing selenium-enriched Cordyceps militaris |
| CN102138436B (en) * | 2010-12-28 | 2012-08-22 | 湖北圣峰药业有限公司 | Method for culturing selenium-enriched Cordyceps militaris |
| CN103030452A (en) * | 2013-01-17 | 2013-04-10 | 李卫旗 | Method for preparing liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris |
| CN104770660A (en) * | 2014-01-13 | 2015-07-15 | 于汇 | Cordyceps sinensis rice with rich cordyceps militaris nutrients and rich medicinal values, and production process thereof |
| CN107043707A (en) * | 2016-11-30 | 2017-08-15 | 广州市瑞硒康生物科技有限公司 | A kind of selenium-enriched cordceps militaris inoculation method |
| CN106957190A (en) * | 2017-04-24 | 2017-07-18 | 广西环江仙草农舍生物科技有限公司 | Cordyceps militaris solid medium |
| CN106978300A (en) * | 2017-04-24 | 2017-07-25 | 广西环江仙草农舍生物科技有限公司 | The preparation method of cordyceps wine |
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Application publication date: 20101201 |