CN107041343B - Method for catching artificially cultured leeches - Google Patents
Method for catching artificially cultured leeches Download PDFInfo
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- CN107041343B CN107041343B CN201611151103.3A CN201611151103A CN107041343B CN 107041343 B CN107041343 B CN 107041343B CN 201611151103 A CN201611151103 A CN 201611151103A CN 107041343 B CN107041343 B CN 107041343B
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- catching
- leech
- adsorption film
- acetate fiber
- fiber cloth
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- 241000545744 Hirudinea Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000001179 sorption measurement Methods 0.000 claims description 35
- 229920006221 acetate fiber Polymers 0.000 claims description 25
- 239000008280 blood Substances 0.000 claims description 24
- 210000004369 blood Anatomy 0.000 claims description 24
- 239000004744 fabric Substances 0.000 claims description 22
- 238000009423 ventilation Methods 0.000 claims description 9
- 238000009395 breeding Methods 0.000 claims description 8
- 230000001488 breeding effect Effects 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 240000008564 Boehmeria nivea Species 0.000 abstract description 2
- 238000012136 culture method Methods 0.000 abstract description 2
- 230000008014 freezing Effects 0.000 abstract description 2
- 238000007710 freezing Methods 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 2
- 239000012528 membrane Substances 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 239000002984 plastic foam Substances 0.000 description 2
- 102000007625 Hirudins Human genes 0.000 description 1
- 108010007267 Hirudins Proteins 0.000 description 1
- 241000237903 Hirudo Species 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 244000078703 ectoparasite Species 0.000 description 1
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 1
- 229940006607 hirudin Drugs 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K80/00—Harvesting oysters, mussels, sponges or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a leech culture method, in particular to an artificial culture leech catching method. The leech catching method is very simple, aquatic plants in the water tank do not need to be removed during catching, the water tank can also keep the original ecological environment after catching, the catching process cannot cause any pollution to the water body, the absorption time of the induced leech is short, the induced leech is not influenced by temperature, and the absorption film can be completely covered by a layer of leech with dense ramie within 1 hour even when the temperature of the water body is at a critical freezing point.
Description
Technical Field
The invention relates to a leech culture method, in particular to an artificial culture leech catching method.
Background
Leeches, commonly known as leeches, also known as leeches, live on the blood of vertebrates or invertebrates. Some are more or less fixedly living on an individual animal, close to the ectoparasites; is a hirudin contained in saliva of aquatic animals with various functions, such as Hirudo, and can be used for inhibiting thrombin activity, preventing fibrin from forming fibrin, and inhibiting thrombosis.
At present, although artificial breeding of leeches is successful, fishing has more difficulties, the fishing efficiency is low, and a large amount of leeches in water can not be captured in a short time.
Application No. 201310421233.4, the patent name is a leech catching method, provides a technical scheme for catching leeches: firstly, adjusting the water depth of a culture pond; (b) removing aquatic weeds and various floating sundries in the culture water area; (c) uniformly sprinkling the nutrient consuming microorganisms with certain solubility in the leech culture water body; (d) covering a layer of black plastic film or foam board on the surface of the water body; (e) after 5 hours, the leeches "adsorbed on the black plastic film or foam board were collected manually.
When leeches are caught and the temperature is low, the water temperature is required to be not lower than 15 ℃, the original ecological environment of a culture water body is required to be damaged, the consumed microorganisms with certain solubility are splashed into the water body, the damage to phytoplankton and plankton in the water body can be caused, a new culture environment needs to be established again after catching is completed, and the restoration cost is high after catching.
Disclosure of Invention
In order to solve the difficulty of the existing leech catching, the invention aims to provide a leech catching method through artificial breeding.
The invention adopts the technical scheme that the artificial breeding leech catching method comprises the following steps,
an adsorption film is covered on the water body of the culture pond close to the water surface, a full-shading black film is covered above the adsorption film, an LED lamp is arranged between the adsorption film and the full-shading black film, the LED lamp emits a green light source, the wavelength of the green light is 550-560nm, the illumination intensity is 5000-5800lux, and the LED lamp is turned on during trapping.
The adsorption film is prepared by taking acetate fiber cloth as a raw material, when the adsorption film is manufactured, a layer of fishy smell-increasing pig blood is uniformly sprayed on the surface of the acetate fiber cloth, when the humidity of the acetate fiber cloth is 85-88%, the acetate fiber is arranged in a closed chamber with the temperature of 45-50 ℃, the closed chamber is quickly vacuumized until the vacuum degree in the closed chamber is-0.03 MPa to-0.025 MPa, after the treatment is carried out for 15-18 minutes, the acetate fiber cloth is placed in a high-pressure chamber for the treatment for 20-23 minutes, and the air pressure in the high-pressure chamber is 0.5-0.8MPa, so that the adsorption film is finally obtained;
when the fishy smell-increased pig blood is prepared, sterilizing the pig blood, inoculating yeast, wherein the inoculation proportion is 2%, and culturing for 15 hours under the conditions that the ventilation ratio is 2-2.5VVM for 0-12 hours and the ventilation ratio is 0.1-0.13VVM for 12-15 hours to obtain the fishy smell-increased pig blood;
the thickness of the adsorption film is between 0.05 and 0.1 cm.
The leech catching method has the advantages that the leech catching method is very simple, only one layer of adsorption film needs to be covered on the water surface in a close mode in the catching process, the leech is attracted to be dragged out of the water surface after being adsorbed, the leech adsorbed on the adsorption film is manually collected, the aquatic plants in the water tank do not need to be removed in the catching process, the water tank can keep the original ecological environment after the leech is caught, the catching process cannot pollute the water body, the attraction leech adsorption time is short, the temperature influence is avoided, the adsorption film can be completely covered by one layer of dense ramie leech within 1 hour even when the water body temperature is at a critical freezing point, in addition, the adsorption film, the full light-shielding black film and the LED lamp can be repeatedly used, and the catching cost is extremely low.
The adsorption membrane prepared by taking the acetate fiber cloth as the raw material has better adhesion performance to leeches, a layer of fishy smell-increasing pig blood is uniformly sprayed on the surface of the acetate fiber cloth and then is placed in a closed chamber at the temperature of 45-50 ℃, the closed chamber is quickly vacuumized until the vacuum degree in the closed chamber is-0.03 MPa-0.025 MPa, after the treatment is carried out for 15-18 minutes, the acetate fiber cloth is placed in a high-pressure chamber with the air pressure of 0.5-0.8MPa for the treatment of 20-23 minutes, so that the fishy smell-increasing pig blood can be fully and completely immersed on the acetate fiber cloth, and the phenomenon of color loss and water quality pollution can not occur when the treated acetate fiber cloth is immersed in water completely;
inoculating yeast after sterilizing the pig blood, ventilating according to different ventilation ratios, and effectively improving the fishy smell of the pig blood, so that the obtained fishy smell-increased pig blood has excellent food calling performance, and can form strong food calling performance on leeches in a water body only by spraying a small amount of the fishy smell-increased pig blood on the surface of an adsorption film;
the leech has strong phototaxis to green light with the wavelength of 550-560nm, the illumination intensity is set to be 5000-5800lux, the leech can attract hidden leech, and meanwhile, the attraction of fishy pig blood is combined, and finally, a better catching effect is realized.
Detailed Description
Embodiment 1, an artificial breeding leech catching method, comprising the following steps,
the water body of the culture pond clings to the water surface and is covered with an adsorption film, a layer of full-shading black film covers above the adsorption film, an LED lamp is arranged between the adsorption film and the full-shading black film, the LED lamp emits a green light source, the wavelength of green light is 550nm, the illumination intensity is 5000lux, and the LED lamp is turned on during trapping.
The adsorption membrane is prepared by taking acetate fiber cloth as a raw material, when the adsorption membrane is manufactured, a layer of fishy smell-enriched pig blood is uniformly sprayed on the surface of the acetate fiber cloth, when the humidity of the acetate fiber cloth is 85%, the acetate fiber cloth is arranged in a closed chamber with the temperature of 45 ℃, the vacuum degree in the closed chamber is quickly vacuumized to be-0.03 MPa, after the treatment is carried out for 15 minutes, the acetate fiber cloth is placed in a high-pressure chamber for the treatment for 20 minutes, and the air pressure in the high-pressure chamber is 0.5MPa, so that the adsorption membrane is finally obtained;
when the fishy smell-increased pig blood is prepared, sterilizing the pig blood, inoculating yeast, wherein the inoculation proportion is 2%, and culturing for 15 hours under the conditions that the ventilation ratio is 2VVM for 0-12 hours and the ventilation ratio is 0.1VVM for 12-15 hours to obtain the fishy smell-increased pig blood;
the thickness of the adsorption film is between 0.05 and 0.1 cm.
Embodiment 2, an artificial breeding leech catching method, comprising the following steps,
an adsorption film is covered on the water body of the culture pond close to the water surface, a full-shading black film is covered above the adsorption film, an LED lamp is arranged between the adsorption film and the full-shading black film, the LED lamp emits a green light source, the wavelength of green light is 560nm, the illumination intensity is 5800lux, and the LED lamp is turned on during trapping.
The adsorption membrane is prepared by taking acetate fiber cloth as a raw material, when the adsorption membrane is manufactured, a layer of fishy smell-enriched pig blood is uniformly sprayed on the surface of the acetate fiber cloth, when the humidity of the acetate fiber cloth is 88%, the acetate fiber is arranged in a closed chamber with the temperature of 50 ℃, the vacuum degree in the closed chamber is quickly vacuumized to be-0.025 Mpa, after 18 minutes of treatment, the acetate fiber cloth is placed in a high-pressure chamber for treatment for 23 minutes, and the air pressure in the high-pressure chamber is 0.8Mpa, so that the adsorption membrane is finally obtained;
when the fishy smell-increased pig blood is prepared, sterilizing the pig blood, inoculating yeast, wherein the inoculation proportion is 2%, and culturing for 15 hours under the conditions that the ventilation ratio is 2.5VVM for 0-12 hours and the ventilation ratio is 0.13VVM for 12-15 hours to obtain the fishy smell-increased pig blood;
the thickness of the adsorption film is between 0.05 and 0.1 cm.
Claims (4)
1. A method for catching artificially cultured leeches, which is characterized by comprising the following steps,
covering an adsorption film on the water body of the culture pond close to the water surface, covering a full-shading black film above the adsorption film, arranging an LED lamp between the adsorption film and the full-shading black film, emitting a green light source by the LED lamp, wherein the illumination intensity is 5000-5800lux, and turning on the LED lamp during trapping;
the adsorption film is prepared by taking acetate fiber cloth as a raw material, when the adsorption film is manufactured, a layer of fishy smell-increasing pig blood is uniformly sprayed on the surface of the acetate fiber cloth, when the humidity of the acetate fiber cloth is 85-88%, the acetate fiber is arranged in a closed chamber with the temperature of 45-50 ℃, the closed chamber is quickly vacuumized until the vacuum degree in the closed chamber is-0.03 MPa to-0.025 MPa, after the treatment is carried out for 15-18 minutes, the acetate fiber cloth is placed in a high-pressure chamber for the treatment for 20-23 minutes, and the air pressure in the high-pressure chamber is 0.5-0.8MPa, so that the adsorption film is finally obtained;
when the fishy smell-increased pig blood is prepared, the pig blood is sterilized and inoculated with yeast, and is cultured for 15 hours under the condition that the ventilation ratio is 2-2.5VVM for 0-12 hours and the ventilation ratio is 0.1-0.13VVM for 12-15 hours, so that the fishy smell-increased pig blood is obtained.
2. The artificial breeding leech catching method according to claim 1,
the green light wavelength is 550-560 nm.
3. The artificial breeding leech catching method according to claim 1,
the proportion of yeast inoculation was 2%.
4. The artificial breeding leech catching method according to claim 1,
the thickness of the adsorption film is between 0.05 and 0.1 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611151103.3A CN107041343B (en) | 2016-12-14 | 2016-12-14 | Method for catching artificially cultured leeches |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611151103.3A CN107041343B (en) | 2016-12-14 | 2016-12-14 | Method for catching artificially cultured leeches |
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| CN107041343A CN107041343A (en) | 2017-08-15 |
| CN107041343B true CN107041343B (en) | 2020-05-19 |
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| CN107897064A (en) * | 2017-11-20 | 2018-04-13 | 广西远程水蛭养殖有限公司 | Propagate the catching method of leech artificially |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103430919B (en) * | 2013-09-17 | 2015-03-11 | 童水明 | Leech catching method |
| CN103621478B8 (en) * | 2013-11-19 | 2016-08-17 | 靖江市明星水蛭养殖专业合作社 | A kind of device attracting and trap leech |
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Effective date of registration: 20200423 Address after: Room 127, Chenzhuang, wangfuying administrative village, Zhongshi street, Yingquan District, Fuyang City, Anhui Province Applicant after: Fuyang Yikai Agricultural Technology Co., Ltd Address before: Wuhe County Creek Zhen Ou Tang Cun 233300 Anhui city of Bengbu province No. 127 Applicant before: WUHE MAOYUAN LEECH ECOLOGICAL BREEDING SPECIALIZED COOPERATIVE |
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