CN107041299A - A kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity - Google Patents
A kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The present invention relates to breeding technique, a kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity is specifically disclosed.With reference to Biology Breeding technologies such as DH breeding techniques, DNA molecular marker technologies, propose a kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity, the torrid zone/subtropical zone germplasm is imported into Temperate zone germplsam by 2 years 4 generations, and obtain the good inbred lines (DH systems) of complete homozygosis, so as to realize that the torrid zone/Subtropical Corn Germplasms quickly introduce Temperate zone germplsam, improve breeding efficiency, it is ensured that the stable sustainable development of breeding work.
Description
Technical field
The present invention relates to breeding technique, specifically, it is related to a kind of Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity
Method.
Background technology
The torrid zone/Subtropical Corn Germplasms are unique corn germplasm monoids, with good lodging resistance, drought tolerance, disease-resistant
Worm property etc., can further improve Temperate maize germplasm economical character as the excellent material for widening Temperate maize Germplasm Bases,
Enhance disease resistance and adaptability.But in Temperate Region in China, plantation has stronger photosensitivity to the torrid zone/Subtropical Corn Germplasms, can produce
Raw breeding time and florescence delay, or even solid can not breed, it is difficult to directly utilize.Need to use introduction and acclimatization and genetic improvement
Its light sensitivity is passivated etc. method.
The conventional torrid zone/Subtropical Corn Germplasms acclimation method is mainly selected by being mixed in different regions at present, by
Domestication is walked, domestication is either directly selected in Temperate Region in China or by a variety of methods such as single cross, three friendships, backcrossing and composite-crossings
The torrid zone/subtropical zone germplasm is imported into Temperate zone germplsam.However, these methods are required to 5-6 or longer time, process is tamed
Slowly, and the remaining germplasm, less efficient of donor system can not be excluded completely.Therefore, a kind of Rapid deactivation torrid zone/Asia of proposition is needed badly
The method of tropical corn germplasm light sensitivity, it is intended to the torrid zone/subtropical zone germplasm is imported into Temperate zone germplsam by 2 years 4 generations, obtained
The corn new germplasm for the complete homozygosis that can be used in Temperate Region in China.
The content of the invention
In order to solve problems of the prior art, technical scheme is as follows:
A kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity, comprises the following steps:
(1) first season, is acceptor, selection and Temperate maize kind according to miscellaneous excellent monoid selection Temperate maize Inbred Lines A
Matter has coordinate force and the torrid zone/subtropical zone germplasm B with the merit such as Resistant, drought-enduring, anti-fall is donor, in the torrid zone/Asia
Torrid areas is planted, and Post flowering is that male parent is hybridized by maternal, Tropical germplsam B of Temperate maize Inbred Lines A, ripe
Afterwards, harvest hybrid seed C;
(2) second season, hybrid seed C is planted in multiple differences such as Beijing, Inner Mongol, Shanxi, Liaoning, Heilungkiang, Gansu
Temperate Region in China, while maize planting haploid inducing line D;In florescence using hybrid seed C as maternal, corn haploid induction
It is that D is hybridized for male parent, after maturation, harvest hybridization fruit ear;And screened according to seed embryo and endosperm top purple mark, select
Go out seed top purple, the colourless seed of embryo for monoploid seed, obtain the monoploid seed E of more than 10000;
(3) third quarter, in the torrid zone/subtropical zone such as Hainan, Guangxi, Yunnan or Beijing, Inner Mongol, Shanxi, Liaoning, black dragon
Monoploid seed E kinds are planted in river, Gansu zone of constant temperature area, and florescence carries out selfing according to haplobiont loose powder situation, ripe
Afterwards, self-fertility fruit ear, as DH pure lines F (DH0 generations) are harvested;
(4) fourth season, selfing is carried out in Temperate Region in China plantation pure lines F (DH0 generations), by serious lodging, male and female it is uncomfortable and
The delay of blooming for producing photosensitized reaction, the pure lines for being unable to selfing are eliminated,
After maturation, will there are serious plant disease and the solid pure lines for being less than 50 to eliminate, harvest self-fertility more than 50
It is sheerly G (DH1 generations);
(5) G will be sheerly in the torrid zone/subtropical zone such as Hainan, Guangxi, Yunnan or Beijing, Inner Mongol, Shanxi, Liaoning, black dragon
River, the plantation of Gansu zone of constant temperature area, Seedling Stage carry out homozygosity identification using DNA molecular marker technology, set up DNA fingerprint figure
Spectrum, analysis pure lines G often uses the genetic distance and miscellaneous excellent monoid of self-mating system with existing Different groups;
According to classification results, cross combination H is assembled with the Inbred Lines of corresponding miscellaneous excellent monoid;
(6) cross combination H is planted in into more than 10 planted in different ecological areas to be identified, selection adaptation is strong, quality is excellent, production
The high cross combination of amount is the Hybrid K of seed selection, and its corresponding DH pure lines G is elite germplasm.
Further, strong adaptability described in step (6) shows as reaching ecotope Major Diseases moderate resistance above rank
Resistance, lodging rate are less than 3% less than 10%, empty bar rate;The quality is excellent to show as crude protein content >=8%, and Crude starch contains
Amount >=72%, crude fat content >=3%, unit weight >=690g/L;The yield height shows as yield higher than control.
The control is local conventional Hybrid.
Preferably, corn haploid induction line D described in step (2) induces haploid induction germplasm from corn
Stock6 Improved lines.
More preferably, the corn haploid induction line D lures 044, Jing Ke You045Huo Jing sections to lure 046 selected from capital section.
Further, the serious plant disease described in step (4) refers mainly to leaf blight, stem rot, ear rot etc..
Preferably, the Inbred Lines described in step (5) are selected from capital 2416, Zheng 58, capital MC01 or capital 92.
Further, technical solution of the present invention is in development, and the torrid zone/subtropical zone selection is Hainan, Guangxi
And Yunnan.The Temperate Region in China selection is Beijing, Gansu, Shanxi, Liaoning, Heilungkiang.
The present invention also provides a kind of Rapid deactivation tropical and semi-tropical population germplasm light sensitivity and imports Temperate maize germplasm
Application of the method in corn breeding.
The beneficial effects of the present invention are:
Deficiency for commonly using breeding method, the present invention combines the Biology Breedings such as DH breeding techniques, DNA molecular marker technology
A kind of technology, it is proposed that method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity, by 2 years 4 generations by the torrid zone/
Subtropical zone germplasm imports Temperate zone germplsam, and obtains the good inbred lines (DH systems) of complete homozygosis, so as to realize tropical sub- heat
Band corn germplasm quickly introduces Temperate zone germplsam, improves breeding efficiency and excellent effect, it is ensured that breeding work is constantly brought forth new ideas
With stablize sustainable development, be a kind of breeding side for the highly effective that tropical and semi-tropical population germplasm is quickly introduced to Temperate zone germplsam
Method.
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It will be appreciated that following real
Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this area
Art personnel can carry out various modifications and replacement in the case of without departing substantially from spirit of the invention and spirit to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material, reagent used etc., unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1
(1) it is acceptor basic material, selection according to miscellaneous excellent monoid selection Temperate maize Inbred Lines capital 2416, Zheng 58 etc.
Tropical germplsam SUWAN 1 is donor, and in Hainan Region plantation, Post flowering is female parent with temperate material capital 2416 and Zheng 58, with heat
Band/subtropical zone material SUWAN 1 is that male parent hybridizes the two.After maturation, harvest hybrid seed 2416 × SUWAN of capital 1, Zheng 58
×SUWAN 1。
(2) 2416 × SUWAN of hybrid seed capital 1,58 × SUWAN of Zheng 1 are planted in Beijing, Shanxi, Liaoning, Heilungkiang
Zone of constant temperature area, at the same plantation induced from corn haploid induction germplasm Stock6 improvement, with seed mark substantially,
The corn haploid induction line capital section for the advantages of growth potential is strong, loose powder is good, pollen amount is sufficient lures 044 etc..
(3) in florescence using 2416 × SUWAN of capital 1 and the hybrid seeds of 58 × SUWAN of Zheng 1 as maternal, corn monoploid
Induction Xi Jing sections lure 044 to be hybridized for male parent.After maturation, harvest hybridization fruit ear.And according to seed embryo and endosperm top purple
Mark is screened, and picks out seed top purple, the colourless seed of embryo for monoploid seed, and 2416 × SUWAN of capital 1 and Zheng 58 ×
SUWAN 1 obtains monoploid seed 4000 respectively.
(4) it will respectively plant, bloom from 2416 × SUWAN of capital 1, the monoploid seeds of 58 × SUWAN of Zheng 1 in Hainan
Phase carries out selfing according to haplobiont loose powder situation.After maturation, self-fertility fruit ear is harvested, as DH is sheerly, capital 2416 ×
SUWAN 1 and 58 × SUWAN of Zheng 1 obtain DH pure lines (2416 × SUWAN of capital 1) DH01, (2416 × SUWAN of capital 1) respectively
DH02, (2416 × SUWAN of capital 1) DH03 etc., (58 × SUWAN of Zheng 1) DH01, (58 × SUWAN of Zheng 1) DH02, (Zheng 58 ×
SUWAN 1) DH03 etc. 120.
(5) in Beijing plantation pure lines (2416 × SUWAN of capital 1) DH01, (2416 × SUWAN of capital 1) DH02, (capital 2416
× SUWAN 1) DH03 etc., (58 × SUWAN of Zheng 1) DH01, (58 × SUWAN of Zheng 1) DH02, (58 × SUWAN of Zheng 1) DH03
Deng, carry out selfing expand it is numerous.By serious lodging, male and female are uncomfortable and produce the delay of blooming of photosensitized reaction, be unable to the pure lines of selfing
Eliminate.After maturation, then will there are the serious plant diseases such as leaf blight, helminthosporium maydis, stem rot, ear rot and fecundity extreme difference (to be less than 50
Grain) pure lines eliminate, pure lines (2416 × SUWAN of capital 1) DH11, the (2416 × SUWAN of capital of harvest self-fertility more than 50
1) DH12, (2416 × SUWAN of capital 1) DH13 etc., (58 × SUWAN of Zheng 1) DH11, (58 × SUWAN of Zheng 1) DH12, (Zheng 58
× SUWAN 1) DH13 etc. 80.
(6) by harvest be respectively derived from 2416 × SUWAN of capital 1,58 × SUWAN of Zheng 1 DH11, DH12, DH13,
80 pure lines such as DH14 are in Hainan plantation, and Seedling Stage carries out homozygosity identification using DNA molecular marker technology, sets up DNA fingerprint
Collection of illustrative plates, analyzes genetic distance and miscellaneous excellent monoid that this 80 pure lines often use self-mating system with existing Different groups.
According to classification results, cross combination is assembled with the Inbred Lines of corresponding miscellaneous excellent monoid, such as derive from capital 2416 ×
The Inbred Lines such as SUWAN 1 DH systems and Zheng 58, capital MC01 assemble (2416 × SUWAN of capital 1) DH11 × Zheng 58, (capital 2416
× SUWAN 1) DH12 × Zheng 58, (2416 × SUWAN of capital 1) DH13 × Zheng 58, (2416 × SUWAN of capital 1) DH11 × capital
The cross combinations 80 such as MC01, (2416 × SUWAN of capital 1) DH12 × capital MC01, (2416 × SUWAN of capital 1) DH13 × capital MC01
It is individual, it is that capital 2416, prosperous 7-2 assemble (58 × SUWAN of Zheng 1) DH11 × capital from Zheng 58 × SUWAN 1DH systems and backbone
2416th, (58 × SUWAN of Zheng 1) DH12 × capital 2416, (58 × SUWAN of Zheng 1) DH13 × capital 2416, (58 × SUWAN of Zheng 1)
The cross combinations 80 such as DH11 × prosperous 7-2, (58 × SUWAN of Zheng 1) DH12 × prosperous 7-2, (58 × SUWAN of Zheng 1) DH13 × prosperous 7-2
It is individual.
(7) this 160 cross combinations are planted in Heilungkiang, Liaoning, Jilin, the Inner Mongol, Beijing, Shanxi, Hebei, river
Identified more than 10 planted in different ecological areas such as south, Shandong, Shaanxi, Xinjiang.
Selection reaches moderate resistance above rank resistance to Major Diseases such as stem rot, QTL mapping, leaf blight, helminthosporium maydis, fallen
Volt rate is less than 10%, empty bar rate be less than 3%, crude protein content >=8%, Crude starch content >=72%, crude fat content >=
3%th, unit weight >=690g/L, yield higher than control etc. excellent (58 × SUWAN of Zheng 1) DH11 × capital 2416 of comprehensive agronomy character,
(58 × SUWAN of Zheng 1) DH12 × capital 2416, (58 × SUWAN of Zheng 1) DH11 × prosperous 7-2, (58 × SUWAN of Zheng 1) DH12 ×
Prosperous 7-2, (2416 × SUWAN of capital 1) DH13 × Zheng 58, (2416 × SUWAN of capital 1) DH13 × capital MC01 cross combinations are seed selection
Hybrid, its corresponding (58 × SUWAN of Zheng 1) DH11, (58 × SUWAN of Zheng 1) DH12, (2416 × SUWAN of capital 1)
DH13 pure lines are to include the Tropical germplsam but elite germplasm without photosensitized reaction.
Embodiment 2
Self-mating system capital SV19.
1st, parental source
Self-mating system capital SV19 is Corn Rearch Center, Beijing Farming & Forestry Research Academy's self bred inbred line, using Suwan1 × PH4CV as base
Plinth material, is formed by DH breeding technique seed selections, and the self-mating system can be to public granting.
2nd, Breeding Process
In October, 2009 completes Suwan1 and PH4CV hybridization in 20 plants of Tropical germplsam Suwan1 of Hainan Screening and Identification.
Hybridize after F1 maturations, mix threshing, be basic material as choosing.
In May, 2010 plants 100 plants of F1 in Beijing with 4000 plants/acre of density, is lured while planting 20 plants of corn monoploid
Dao Xi capital section lures 045.In florescence, the two is hybridized.After maturation, harvest 80 plants of fruit ear of hybridization.And according to seed embryo and
Endosperm top purple mark is screened, and picks out seed top purple, the colourless seed of embryo for monoploid seed 1100.
In October, 2010, florescence dissipated according to haplobiont in Hainan by 1000 monoploid seeds whole monoseedings
Powder situation carries out selfing, obtains DH and is sheerly 112 plants.
In May, 2011 is planted in Beijing will obtain 112 plants of DH pure lines.Will lodging more than 10% and generation light
The delay of blooming of quick reaction, male and female are uncomfortable, be unable to selfing pure lines eliminate.After maturation, then will there are leaf blight, helminthosporium maydis, stem rot
The serious plant diseases such as disease, ear rot and the pure lines of fecundity extreme difference (being less than 50) are eliminated, harvest self-fertility more than 50
DH is sheerly 20 plants.
Plant 20 plants of DH pure lines in October, 2011 in Hainan, Seedling Stage is carried out using DNA molecular marker assistant breeding technology
Homozygosity is identified, sets up DNA fingerprinting, 20 plants of DH pure lines of analysis and the genetic distance and miscellaneous excellent class of existing Inbred Lines
Group, while carrying out expanding numerous and being assembled with existing conventional self-mating system PH6WC.
In May, 2012, will assemble cross combination be planted in Heilungkiang, Liaoning, Jilin, the Inner Mongol, Beijing, Shanxi, Hebei,
Identified more than 10 planted in different ecological areas such as Henan, Shandong, Shaanxi, Xinjiang.Selection to stem rot, QTL mapping, leaf blight,
The Major Diseases such as helminthosporium maydis reach that moderate resistance above rank resistance, lodging rate are that less than 3%, crude protein contains less than 10%, empty bar rate
Amount >=8%, Crude starch content >=72%, crude fat content >=3%, unit weight >=690g/L, yield are higher than comprehensive agronomies such as controls
The excellent PH6WC of character × capital SV19 cross combinations are the Hybrid of seed selection, and its corresponding pure lines capital SV19 is good species
Matter.
3rd, feature
Seedling leaf sheath aubergine, flower pesticide lavender, leaf green, plant type half is compact, plant height 176cm, fringe position 98cm, tassel
Branch 5-7, filigree pale red, spike length 17.2cm, the thick 4.9cm of fringe, the row of head progeny row 16, cartridge type fringe, cob is red, and seed is pale yellow
Color, half Hard grain type.The high Resistance To Helminthosporium Turcicum of the self-mating system, helminthosporium maydis, curved spore leaf spot, lodging resistance are good.Pollen amount is big, the loose powder time
It is long, it is suitable for use as male parent.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (9)
1. a kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity, it is characterised in that comprise the following steps:
(1) first season, is acceptor according to miscellaneous excellent monoid selection Temperate maize Inbred Lines A, selection has with Temperate maize germplasm
The coordinate force and torrid zone/subtropical zone germplasm B with Resistant, drought-enduring, anti-fall merit is donor, in the torrid zone/subtropical zone
Area is planted, and Post flowering is that male parent is hybridized by maternal, Tropical germplsam B of Temperate maize Inbred Lines A, after maturation, harvest
Hybrid seed C;
(2) second season, hybrid seed C is planted in multiple different Temperate Region in China, while maize planting haploid inducing line D;
By maternal, corn haploid induction line D of hybrid seed C be that male parent is hybridized in florescence, it is ripe after, harvest allocarpy
Fringe;And screened according to seed embryo and endosperm top purple mark, seed top purple, the colourless seed of embryo are picked out for monoploid seed
Grain, the monoploid seed E that acquisition is more than 10000;
(3) third quarter, monoploid seed E kinds are planted in the torrid zone/subtropical zone, florescence is according to haplobiont loose powder situation
Carry out after selfing, maturation, harvest self-fertility fruit ear, as DH pure lines F (DH0 generations);
(4) fourth season, selfing is carried out in Temperate Region in China plantation pure lines F (DH0 generations), by serious lodging, male and female are uncomfortable and produce
The delay of blooming of photosensitized reaction, the pure lines for being unable to selfing are eliminated, it is ripe after, will have serious plant disease and solid be less than 50 pure
System eliminates, the pure lines G (DH1 generations) that harvest self-fertility is more than 50;
(5) pure lines G is planted in the torrid zone/subtropical zone or Temperate Region in China, Seedling Stage is carried out pure using DNA molecular marker technology
Right identification, sets up DNA fingerprinting, analysis pure lines G is with existing Different groups often with the genetic distance and miscellaneous excellent class of self-mating system
Group;
According to classification results, cross combination H is assembled with Inbred Lines;
(6) cross combination H is planted in into more than 10 planted in different ecological areas to be identified, selection adaptation is strong, quality is excellent, yield is high
Cross combination be seed selection Hybrid K, its corresponding DH pure lines G is elite germplasm.
2. according to the method described in claim 1, it is characterised in that strong adaptability is shown as to ecotope described in step (6)
Major Diseases reach that moderate resistance above rank resistance, lodging rate are less than 3% less than 10%, empty bar rate;The quality is excellent to be shown as
Crude protein content >=8%, Crude starch content >=72%, crude fat content >=3%, unit weight >=710g/L;The yield height performance
It is that yield is higher than control.
3. method according to claim 2, it is characterised in that the control is local conventional Hybrid.
4. the method according to claims 1 to 3 any one, it is characterised in that corn monoploid lures described in step (2)
Lead the Improved lines for being D from corn induction haploid induction germplasm Stock6.
5. method according to claim 4, it is characterised in that the corn haploid induction line D lures 044 selected from capital section,
You045Huo Jing sections of capital section lure 046.
6. the method according to claims 1 to 3 any one, it is characterised in that the serious plant disease master described in step (4)
To be leaf blight, helminthosporium maydis, stem rot or ear rot.
7. method according to claim 3, it is characterised in that the Inbred Lines described in step (5) be selected from capital 2416,
Zheng 58, capital MC01 or capital 92.
8. the method according to claims 1 to 3 any one, it is characterised in that the torrid zone/subtropical zone is selected from sea
South, Guangxi and Yunnan.
9. the method according to claims 1 to 3 any one, it is characterised in that the Temperate Region in China is selected from Beijing, interior
Illiteracy, Shanxi, Liaoning, Heilungkiang, Gansu.
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CN109430046A (en) * | 2018-11-12 | 2019-03-08 | 德宏州农业技术推广中心(德宏州农业科学研究所) | Quickly introduce the germ plasm resource modification method in the torrid zone, subtropical zone corn disease-resistant gene |
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CN110833026A (en) * | 2019-11-27 | 2020-02-25 | 广西壮族自治区农业科学院玉米研究所 | Breeding method of silage corn male parent |
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CN109430046A (en) * | 2018-11-12 | 2019-03-08 | 德宏州农业技术推广中心(德宏州农业科学研究所) | Quickly introduce the germ plasm resource modification method in the torrid zone, subtropical zone corn disease-resistant gene |
CN110122318A (en) * | 2019-05-06 | 2019-08-16 | 贵州省旱粮研究所 | A method of passivation corn Suwan self-mating system light sensitivity |
CN110833026A (en) * | 2019-11-27 | 2020-02-25 | 广西壮族自治区农业科学院玉米研究所 | Breeding method of silage corn male parent |
CN116267583A (en) * | 2023-03-15 | 2023-06-23 | 北京市农林科学院 | Precise introduction and identification method for excellent characters of corn |
CN117016372A (en) * | 2023-03-15 | 2023-11-10 | 北京市农林科学院 | Method for creating excellent corn germplasm and application thereof |
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