CN109430046A - Quickly introduce the germ plasm resource modification method in the torrid zone, subtropical zone corn disease-resistant gene - Google Patents
Quickly introduce the germ plasm resource modification method in the torrid zone, subtropical zone corn disease-resistant gene Download PDFInfo
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- CN109430046A CN109430046A CN201811372805.3A CN201811372805A CN109430046A CN 109430046 A CN109430046 A CN 109430046A CN 201811372805 A CN201811372805 A CN 201811372805A CN 109430046 A CN109430046 A CN 109430046A
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- 240000008042 Zea mays Species 0.000 title claims abstract description 58
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 58
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 47
- 235000005822 corn Nutrition 0.000 title claims abstract description 47
- 201000010099 disease Diseases 0.000 title claims abstract description 40
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 40
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 34
- 238000002715 modification method Methods 0.000 title claims abstract description 18
- 238000009395 breeding Methods 0.000 claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 23
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims abstract description 11
- 235000009973 maize Nutrition 0.000 claims abstract description 11
- 238000005516 engineering process Methods 0.000 claims abstract description 9
- 238000012216 screening Methods 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims description 33
- 241000196324 Embryophyta Species 0.000 claims description 13
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 claims description 10
- 230000006698 induction Effects 0.000 claims description 9
- 230000000306 recurrent effect Effects 0.000 claims description 9
- 229960001338 colchicine Drugs 0.000 claims description 5
- 238000009396 hybridization Methods 0.000 claims description 5
- 230000002068 genetic effect Effects 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 238000002372 labelling Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 210000000349 chromosome Anatomy 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 230000008676 import Effects 0.000 claims 1
- 230000006872 improvement Effects 0.000 abstract description 9
- 238000011161 development Methods 0.000 abstract description 3
- 208000003643 Callosities Diseases 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 208000037920 primary disease Diseases 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of germ plasm resource modification methods for quickly introducing the torrid zone, subtropical zone corn disease-resistant gene, it is related to agricultural technology field, this quickly introduces the torrid zone, the germ plasm resource modification method of subtropical zone corn disease-resistant gene fully utilizes various advanced breeding techniques and precisely improves various breeding objectives, the development germ plasm resource of precise and high efficiency improves, time used is only 2 years, time-consuming short and accurate improvement in 3~4 years is lasted compared to traditional breeding technology, improves breeding efficiency.Can carry out the follow-up works such as variety certification after the 5th season obtained cross combination screening simultaneously by the breeding technique, while stable disease-resistant maize self-mating system accurately improve corn germ plasm resource.
Description
Technical field
It is the present invention relates to agricultural technology field, in particular to a kind of to quickly introduce the torrid zone, subtropical zone corn disease-resistant gene
Germ plasm resource modification method.
Background technique
Corn is one of most important cereal crops in the world, and the biology such as arid and disease and abiotic stress are to jade at present
The growth of rice causes one of serious influence and the principal element of the corn underproduction.The yield of Chinese maize kind at this stage
There are larger difference compared with the U.S., it is mainly reflected in that Germplasms are narrow and disease resistance difference etc..Therefore in widening
The genetic diversity of state's corn germ plasm resource is always the main direction of studying of Chinese maize breeding, introduces and utilizes Exotic Germplasm
Resource (especially tropical and subtropical maize germplasm) improvement local resource is to widen Chinese maize Germplasm Bases, break through breeding bottle
The effective measures of neck.In recent years, the corn growth season long-term high temperature and humidity of Southwest China main producing region, causes pest and disease damage to take place frequently,
Wherein especially prominent with the harm of graywall, the disease has become the primary disease of the southwest corn underproduction at present.Long-term life
Produce practice have shown that, cultivate and promote disease-resistant variety fundamentally can prevent grey leaf spot disease of maize from spreading, while be also it is most economical, effective
Measure, and the breeding of disease-resistant variety depends on the parsing of bacterial blight mechanism.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of kinds for quickly introducing the torrid zone, subtropical zone corn disease-resistant gene
Matter resource modification method fully utilizes various advanced breeding techniques and precisely improves various breeding objectives, the development of precise and high efficiency
Germ plasm resource improvement, the time used is only 2 years, lasts time-consuming short and accurate improvement in 3~4 years compared to traditional breeding technology, improves
Breeding efficiency.
To achieve the above object, the present invention provides technical solution below:
This quickly introduces the torrid zone, the germ plasm resource modification method of subtropical zone corn disease-resistant gene includes haploid breeding skill
Art: Haploid production plant is induced using plant tissue culture technique (such as vitro anther culture), then is made by certain means
Genome is doubled and (is such as handled with colchicine), to make plant recovery normal chromosomal number;Molecular mark is
The characteristics of using molecular labeling and determining objective trait gene close linkage (QTL), is marked by detection molecules, be can be detected
The presence of target gene achievees the purpose that selection target character;Backcrossing is to be hybridized appointing for first filial generation and two parents
Method reinforces the trait expression of hybrid individual, the especially backcrossing with recessive parent using the method for backcrossing, examines first filial generation
Genotype, the offspring caused by Backcrossing methods is known as backcross hybrid.
This quickly introduces the torrid zone, specific step is as follows for the germ plasm resource modification method of subtropical zone corn disease-resistant gene:
(1) first season selects two parents' materials, and one is the torrid zone, the disease-resistant high-grade maize self-mating system in subtropical zone (has height
Coordinate force and high disease-resistant characteristic), another (has high-combining ability, that is, commercial value, exists for high sense corn material recurrent parent
Susceptible risk is badly in need of improvement), it is assembled by hybridization and generates F1 generation seed, respectively 50% gene containing parents;
(2) second season, susceptible material hybridizes as recurrent parent with F1 generation seed assemble after obtain BC1F1 group, pass through
This, which is assembled, obtains containing the more genetic fragments of recurrent parent;
(3) third quarter, using corn induction, system induces BC1F1 group, generates induction group, is screened by range estimation
Induce successful corn kernel;
(4) fourth season plants the above-mentioned corn kernel screened, and passes through colchicine (or other inductive technologies)
It carries out group to double, this step filters out the induced material successfully doubled;
(5) the 5th seasons planted the induced material that above-mentioned success doubles, and plant head progeny row, while planting other superior corns
Self-mating system is used as and is measured group.
Mainly carry out following 3 work in step (5):
A. corn sample collection (blade) is carried out during 6~8 leaves of corn, every row acquires 3 plants of samples, auxiliary using molecule
Helping property breeding technique screening the segment containing disease-resistant gene head progeny row (i.e. using forefathers study in identify come disease-resistant QTL sieve
Choosing), the head progeny row for being free of disease-resistant gene is rejected, otherwise is retained;
B. identify whether above-mentioned reserved materials are genuine monoploid material in tasseling stage, the material of removal retains vacuum material;
C. the material of above-mentioned reservation, a part of hybridize assembling with the excellent material that is measured, the excellent hybridization of breeding is beautiful
Meter Zu He, another part selfing save.
Be using the beneficial effect of above technical scheme: this quickly introduces the germplasm in the torrid zone, subtropical zone corn disease-resistant gene
Resource modification method fully utilizes various advanced breeding techniques and precisely improves various breeding objectives, the development germplasm of precise and high efficiency
Resource improvement, the time used is only 2 years, lasts time-consuming short and accurate improvement in 3~4 years compared to traditional breeding technology, improves and educate
Kind efficiency.The follow-up works such as variety certification can be carried out after the 5th season obtained cross combination screening simultaneously by the breeding technique,
Simultaneously or stable disease-resistant maize self-mating system accurately improves corn germ plasm resource.
Detailed description of the invention
A specific embodiment of the invention is described in further detail with reference to the accompanying drawing.
Fig. 1 is the schematic diagram that the present invention quickly introduces tropical, subtropical zone corn disease-resistant gene germ plasm resource modification method.
Specific embodiment
The invention will now be described in detail with reference to the accompanying drawings quickly introduces the torrid zone, the germ plasm resource of subtropical zone corn disease-resistant gene changes
The preferred embodiment of good recipe method.
Fig. 1 shows the present invention and quickly introduces the specific of tropical, subtropical zone corn disease-resistant gene germ plasm resource modification method
Embodiment:
As shown in Figure 1, this quickly introduces the torrid zone, the germ plasm resource modification method of subtropical zone corn disease-resistant gene includes:
Haploid breeding technology: it is planted using plant tissue culture technique (such as vitro anther culture) induction Haploid production
Strain, then so that genome is doubled and (is such as handled with colchicine) by certain means, to make plant recovery normal chromosomal number.
The characteristics of molecular mark is using molecular labeling and decision objective trait gene close linkage (QTL),
It is marked by detection molecules, can be detected the presence of target gene, achieve the purpose that selection target character, had quick, quasi-
Really, the advantages of not interfered by environmental condition.
Backcrossing is that first filial generation and two parents appoint the method hybridized to be called backcrossing.In genetics research, often
Reinforce the trait expression of hybrid individual, the especially backcrossing with recessive parent using the method for backcrossing, it is to examine first filial generation
Genotype important method.The offspring caused by Backcrossing methods is known as backcross hybrid.
This quickly introduces the torrid zone, specific step is as follows for the germ plasm resource modification method of subtropical zone corn disease-resistant gene:
(1) first season selects two parents' materials, and one is the torrid zone, the disease-resistant high-grade maize self-mating system in subtropical zone (has height
Coordinate force and high disease-resistant characteristic), another (has high-combining ability, that is, commercial value, exists for high sense corn material recurrent parent
Susceptible risk is badly in need of improvement), it is assembled by hybridization and generates F1 generation seed, respectively 50% gene containing parents;
(2) second season, susceptible material hybridizes as recurrent parent with F1 generation seed assemble after obtain BC1F1 group, pass through
This, which is assembled, obtains containing the more genetic fragments of recurrent parent;
(3) third quarter, using corn induction, system induces BC1F1 group, generates induction group, is screened by range estimation
Induce successful corn kernel;
(4) fourth season plants the above-mentioned corn kernel screened, and passes through colchicine (or other inductive technologies)
It carries out group to double, this step filters out the induced material successfully doubled;
(5) the 5th seasons planted the induced material that above-mentioned success doubles, and plant head progeny row, while planting other superior corns
Self-mating system is used as and is measured group.
Mainly carry out following 3 work in step (5):
A. corn sample collection (blade) is carried out during 6~8 leaves of corn, every row acquires 3 plants of samples, auxiliary using molecule
Helping property breeding technique screening the segment containing disease-resistant gene head progeny row (i.e. using forefathers study in identify come disease-resistant QTL sieve
Choosing), the head progeny row for being free of disease-resistant gene is rejected, otherwise is retained;
B. identify whether above-mentioned reserved materials are genuine monoploid material in tasseling stage, the material of removal retains vacuum material;
C. the material of above-mentioned reservation, a part of hybridize assembling with the excellent material that is measured, the excellent hybridization of breeding is beautiful
Meter Zu He, another part selfing save.
The above are merely the preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art,
Without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.
Claims (3)
1. a kind of germ plasm resource modification method for quickly introducing the torrid zone, subtropical zone corn disease-resistant gene, it is characterised in that: described fast
Speed imports the torrid zone, the germ plasm resource modification method of subtropical zone corn disease-resistant gene includes haploid breeding technology: utilizing plant group
Culture technique induction Haploid production plant is knitted, then doubles genome by certain means, to keep plant recovery normal
Chromosome number;The characteristics of molecular mark is using molecular labeling and decision objective trait gene close linkage, passes through
Detection molecules label, can be detected the presence of target gene, achievees the purpose that selection target character;Backcrossing is first filial generation and two
A parent's appoints the method hybridized, reinforces the trait expression of hybrid individual using the method for backcrossing, especially with it is hidden
The backcrossing of property parent, examines the genotype of first filial generation, the offspring caused by Backcrossing methods is known as backcross hybrid.
2. the germ plasm resource modification method according to claim 1 for quickly introducing the torrid zone, subtropical zone corn disease-resistant gene,
Be characterized in that: it is described quickly introduce the torrid zone, subtropical zone corn disease-resistant gene germ plasm resource modification method specific step is as follows:
(1) first season selects two parents' materials, and one is the torrid zone, the disease-resistant high-grade maize self-mating system in subtropical zone, another is height
Feel corn material recurrent parent, is assembled by hybridization and generate F1 generation seed, respectively 50% gene containing parents;
(2) second season, susceptible material hybridizes as recurrent parent with F1 generation seed assemble after obtain BC1F1 group, pass through the group
With obtaining more genetic fragments containing recurrent parent;
(3) third quarter, using corn induction, system induces BC1F1 group, generates induction group, passes through range estimation screening induction
Successful corn kernel;
(4) fourth season plants the above-mentioned corn kernel screened, and carries out group by colchicine and double, this step sieve
Select the induced material successfully doubled;
(5) the 5th seasons planted the induced material that above-mentioned success doubles, and plant head progeny row, while planting other superior corn selfings
System is used as and is measured group.
3. the germ plasm resource modification method according to claim 2 for quickly introducing the torrid zone, subtropical zone corn disease-resistant gene,
Be characterized in that: mainly carry out following 3 work in the step (5): a. carries out corn sample during 6~8 leaves of corn and adopts
Collect (blade), every row acquires 3 plants of samples, using the head progeny row of the complementary breeding technique screening segment containing disease-resistant gene of molecule, rejects
Head progeny row without disease-resistant gene, on the contrary it retains;B. it identifies whether above-mentioned reserved materials are genuine monoploid material in tasseling stage, goes
The material removed retains vacuum material;C. the material of above-mentioned reservation, a part of hybridize assembling with the excellent material that is measured, breeding
Excellent hybrid maize combination, another part selfing save.
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5749169A (en) * | 1995-06-07 | 1998-05-12 | Pioneer Hi-Bred International, Inc. | Use of the indeterminate gametophyte gene for maize improvement |
CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
US20150173318A1 (en) * | 2012-09-09 | 2015-06-25 | Y.E. Vigor.Corn | Novel genetic factor capable of increasing yield in maize and method thereof |
US20170073701A1 (en) * | 2015-09-10 | 2017-03-16 | Monsanto Technology Llc | Methods for producing corn plants with downy mildew resistance and compositions thereof |
CN107041299A (en) * | 2017-05-26 | 2017-08-15 | 北京市农林科学院 | A kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity |
-
2018
- 2018-11-12 CN CN201811372805.3A patent/CN109430046A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5749169A (en) * | 1995-06-07 | 1998-05-12 | Pioneer Hi-Bred International, Inc. | Use of the indeterminate gametophyte gene for maize improvement |
CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
US20150173318A1 (en) * | 2012-09-09 | 2015-06-25 | Y.E. Vigor.Corn | Novel genetic factor capable of increasing yield in maize and method thereof |
US20170073701A1 (en) * | 2015-09-10 | 2017-03-16 | Monsanto Technology Llc | Methods for producing corn plants with downy mildew resistance and compositions thereof |
CN107041299A (en) * | 2017-05-26 | 2017-08-15 | 北京市农林科学院 | A kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity |
Non-Patent Citations (2)
Title |
---|
段民孝等: "快速钝化玉米热带亚热带种质光敏性方法及其育种利用研究" * |
王连生等: "外来玉米种质的抗病和抗逆性选育" * |
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Application publication date: 20190308 |