CN110122318A - A method of passivation corn Suwan self-mating system light sensitivity - Google Patents
A method of passivation corn Suwan self-mating system light sensitivity Download PDFInfo
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- CN110122318A CN110122318A CN201910372606.0A CN201910372606A CN110122318A CN 110122318 A CN110122318 A CN 110122318A CN 201910372606 A CN201910372606 A CN 201910372606A CN 110122318 A CN110122318 A CN 110122318A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of methods for being passivated corn Suwan self-mating system light sensitivity, are directly tamed using photosensitive corn Suwan self-mating system in northern production of hybrid seeds area, with the tame and docile method in south choosing north, obtain 1 generation of domestication；It will tame for 1 generation by head progeny row plantation acquisition 2 generations of domestication, stable seed must be tamed by continuing domestication 2~3 times, then identified, obtain the corn Suwan self-mating system to Day neutral.The application makees domestication material with photosensitive corn Suwan self-mating system QB48, T32, GD909, XZ50612, S611, with the tame and docile method in south choosing north, make Suwan self-mating system light sensitivity insensitiveness, setting percentage improves 42~53%, hybrid seed yield improves 51.3~187.6%, and flowering time does sth. in advance 5~15d, shortens breeding time, save the cost has been filled up and has produced the blank that upper excellent Suwan self-mating system is directly tamed in the north.
Description
Technical field
The invention belongs to crop breeding technical field more particularly to a kind of passivation corn Suwan self-mating system light sensitivity
Method.
Background technique
Corn (Classification system: ZeamaysL.) is grass family Zea annual herb plant.Also known as maize, stick
Son, maize, corn, corn, maize, maize, maize, luxuriant reed etc., northeast Liaoning words claim pearl grain, and Teochew claims SEMEN COICIS,
Guangdong language is known as maize, and it is the first in the world generalized grain crop that the south of Fujian Province language, which is referred to as kind wheat, the referred to as king of feed.It is simultaneously also benefit
Earliest and one of the most significant crop of effect is obtained with hybrid vigour, and cultivated area and total output are only second to rice and wheat.
Corn is all known as always life prolonging food, and protein rich in, fat, vitamin, microelement, cellulose etc. have
Develop high nutrition, the great potential of high biological function food, and corn yield is high, it is adaptable, seed, stem when nutrition it is rich
Richness is the high-quality feed of various domestic animals.Therefore, develop maize production to Ensuring Food Safety and promote animal husbandry development have to
Close important role.But since its heredity is complex, variation type is abundant, and there is the periods in conventional breeding process
It is too long, the coefficient of variation is excessive, influence offspring development the shortcomings that.
Southwestern China ecological environments of the mountain area is complicated, soil depletion, at the same corn growth frequently occur arid early period, after
Phase a run of wet weather causes the requirement in production to kind high, such as resistance and quality, to solve the problems, such as that it certainly will just be utilized and come from
Tropical and subtropical maize germplasm, and Suwan germplasm is that tropical and subtropical maize germplasm is important represents one of germplasm, with disease resistance, degeneration-resistant
The advantages that property and seed commodity are good, is the indispensable germplasm in the ecotope, the Heterosis of the ecotope is generally
Temperate zone germplsam × tropical and subtropical maize germplasm such as makees female parent in seed production process with Temperate zone germplsam self-mating system, later period plant
Pinta and fringe kernel rot phenomenon are heavier；Female parent is such as made with tropical and subtropical zone self-mating system, breeding time is generally long compared with Temperate zone germplsam, is broadcasting
The wrong phase is needed when kind, that is, needs to adjust sowing gap stage.
Currently, the technical solution for planting progress corn germplasm improvement that shuttles about strange land has a small amount of document report, such as:
1, document " Rapid deactivation corn tropical and subtropical maize germplasm light sensitivity method and its breeding utilization research " (Duan Minxiao,
Liu Xinxiang, Zhang Huasheng etc., seed, 2018-08-25), for tropical and subtropical maize germplasm, in Temperate Region in China, there are light sensitivity, needs
The problems such as time is long, process is slow, is donor by receptor, Tropical germplsam of Temperate zone germplsam, has carried out the Rapid deactivation corn torrid zone
Subtropical zone germplasm light sensitivity technique study；By being planted using Seed Propagation in Southern Winter, the south and the north region, in conjunction with corn DH
(DoubledHaploid) breeding technique obtained directly can both using in the new material that temperate zone utilizes through 2 years 4 generations
In breeding new varieties, it can also be used as new material broadening Temperate maize Germplasm Bases；It was verified that the method for research is practical
Effectively, the utilization process of China's tropical and semi-tropical population germplasm will be accelerated.First from the selection of material, the document uses temperate zone
The PH4CV of Day neutral and tropical photosensitive Suwan1, assembles hybrid seed F1, after maturation harvests, mixes threshing, is base as choosing
Plinth material, belongs to Genetic improvement, the feature of Suwan1 cannot be retained at the passivation torrid zone, subtropical zone light sensitivity, to solution
Productive target is indefinite.Secondly, the document selects the means combined with DH breeding technique, but the material of breeding using pedigree
Also it is not used in production.Again, the document is in Hainan and Beijing two places Breeding of Inbred Lines, and the cenospecies then assembled is in the north
Point identification, there are no really in production.
2, patent application CN201810776170.7 discloses the circulation modification method in a kind of corn Suwan germplasm group,
By select production on using preferable Elite inbred hybridize or be returned constructional base group after, using improvement i and ii pedigree
Basic population continuous multi-generation selfed breeding corn inbred line of the means of back-and-forth method selection and shuttle breeding from establishment, polymerization
The excellent characteristics of Suwan corn germplasm；The self-mating system Q2232 that the application is bred as according to the above method is compared with QR273, QB48, T32 group
The combination matched increases production 8.6%, 13.2%, 16.55% respectively, illustrates that this method effectively raises yield combining ability, and show
Resistance to lean, the disease-resistant, wide adaptability of drought resisting and plant type is good, it is breeding that breeding and the production application for improving Southwestern China mountain area are horizontal
The corn hybrid seed for adapting to Southwestern China mountain area is laid a good foundation.Firstly, the patent application is each character progress for material
Coordinate force is improved and improved, does not refer to and light sensitivity is passivated, belongs to Genetic improvement.Change secondly, the patent application uses
The selection of good i Pedigree selection method and improvement ii Pedigree selection method selection, i.e. F1 generation do not select, mixed pollination, and F2 or F1BC1 generation start
In individual plant selfing, head progeny row plantation and selection, F2 generation, start later in strange land shuttle plantation selection, i.e., by the way of hybridization and backcrossing
And it is selected in strange land.
It follows that above-mentioned two documents be by plantation improvement come solve the problems, such as innovative material, improve coordinate force, but
To carry out seed production in Southwestern China mountain area, production seed quality is poor, low output, and not due to its climatic factor
Stablize, will lead to the production of hybrid seeds underproduction or total crop failure；And seed production is carried out in the north, and because the confrontation of tropical and subtropical zone Suwan kind is photosensitive
It is perceptual strong, it cannot be directly used to seed production, this is the problem of long-standing problem Southwest Mountainous Areas corn breeding is with seed production is restricted.
Therefore, domestication produces upper superior corn Suwan self-mating system, makes its light sensitivity insensitiveness, carries out seed production in the north, makes to improving
Kind yield and quality saves production cost and is of great significance.But using the upper superior corn Suwan self-mating system of production in north
Side's directly domestication yet there are no report.
Summary of the invention
The present invention is in order to solve the above technical problems, provide a kind of method for being passivated corn Suwan self-mating system light sensitivity.This
Application, as domestication material, is broadcast using photosensitive corn Suwan self-mating system QB48, T32, GD909, XZ50612, S611 using fixation
Kind day, the method progress " southern that north is selected to tame and docile " that pollination time limit, the plantation of big group, individual plant selfing or strain cross are set, make Suwan certainly
Friendship is that can be normally carried out seed production in the north after light sensitivity insensitiveness, in the setting percentage of north after taming Suwan germplasm
42~53% are improved, breeding time is shortened, flowering time does sth. in advance 5~15d, to hybrid seed yield and quality is improved, saves production
Cost is of great significance, and has filled up and has produced the blank that upper excellent Suwan self-mating system is directly tamed in the north, is sub- using the torrid zone
The corn hybrid seed that tropical Suwan germplasm breeding adapts to Southwestern China mountain area is laid a good foundation.
In order to reach purpose described above, the invention adopts the following technical scheme:
A method of passivation corn Suwan self-mating system light sensitivity, comprising the following steps:
(1) straight in northern production of hybrid seeds area as domestication material using the photosensitive corn Suwan self-mating system of Southwest China cenospecies
Domestication is connect, " south is carried out using the method for fixed sowing day, setting pollination time limit, the plantation of big group, individual plant selfing or strain cross
Tame and docile in choosing north ", obtain 1 generation seed of domestication；
(2) it the domestication 1 generation seed that step (1) obtains is carried out number after point fringe threshing saves seed to plant by head progeny row, often
A head progeny row plants 30 plants or more, is tamed according to " taming and dociling in south choosing north " method of step (1), compares upper 1 year pollination date,
The plant that every primary selection of domestication shifts to an earlier date 1~2d is selfed, and selection setting percentage retains up to 30% or more fruit ear when harvest, obtains
2 generation seeds must be tamed；
(3) the domestication 2 generation seed obtained in step (2) is continued domestication 2~3 times according to the method for step (2), is tamed and dociled
Change mostly for seed, the domestication for being screened out from it reduction in the life period and male and female coordination stablizes seed, which is stablized seed and is carried out
Identification, and leave light sensitivity insensitiveness and setting percentage normally tame stablize seed as original cenospecies parent, acquisition for giving birth to
It produces and to the corn Suwan self-mating system of Day neutral；
In step (3), the method for the identification are as follows: make male parent and domestication material pair using the stable self-mating system of the domestication
The maternal self-mating system answered is hybridized, and the self-mating system after the cenospecies assembled and domestication passes through field in Southwestern China different ecological point
Between field plot test and DNA identification, compared and compared with former cenospecies and self-mating system, in addition to light sensitivity, other characters hybridize with former
Kind and self-mating system do not have discrepant domestication to stablize self-mating system, are left as the parent of former cenospecies.The setting percentage just refers to
The setting percentage of self-mating system is 61~98% after domestication.It can guarantee that the cenospecies assembled after domestication hybridizes with original by this method identification
Kind is completely the same, finally solves the problems, such as that seed produces.
In step (3), the method for the screening are as follows: after the seed plantation in domestication mostly generation, investigate pollination day in florescence
The head progeny row that phase can normally pollinate before August 10th, and breeding time shifts to an earlier date 5d's or more compared with former self-mating system；It is adjusted in head progeny row
The male and female flowering period phase for looking into 10 single plants is on the same day or female flower is 1~2d more early than male flower is advisable, and guarantees single plant and normally pollinates
Head progeny row is selected in, and the seed of harvest is stayed and stablizes seed as domestication.
Further, in step (1), the direct domestication refers to using photosensitive corn Suwan self-mating system in north,
It is allowed to tame light sensitivity, not every the material blood relationship for closing any other source.
Further, in step (1), the photosensitive corn Suwan self-mating system refers to be excellent in Southwest China itself
And photosensitive corn Suwan self-mating system QB48, T32, GD909, XZ50612 and S611 of tropical and subtropical zone that general combining ability is good.
Further, in step (1), the cenospecies, which refers to, plants its yielding ability, yield stability, suitable in Southwest Mountainous District in China
Ying Xingjun shows excellent gold list 999, Guidan No.8, magnificent 818, gold and jade No. 5 cenospecies of 908 and Xing Nongdan.
Further, in step (1), the north production of hybrid seeds area refers to that latitude is 37~39 °, height above sea level is 1300~1600m
Hexi Corridor as domestication place.The place is the core area of national seed production, and the sunshine-duration is long, is most suitable for corn
Growth, the relatively other regions of yield are higher, and tame for photosensitive material in this region, solve the problems, such as that seed produces.
Further, in step (1), the fixed sowing date is: being sowed within 19th~21 in April, the sowing day
Phase to light and temperature condition of the corn during mature is conducive to achieve the purpose that light sensitive passivating material.This sowing date and locality
The large area seed production sowing time is consistent relatively；Simultaneously in corn growth season and local sunshine tight association.
Further, in step (1), the setting pollination time limit is: according to the breeding time of self-mating system, pollination date being existed
Mid-July to early August, what is bloomed after August 10th will no longer pollinate.Since the place of selection domestication was at annual August 10th
The variation of later temperature and sunshine makes the viability decline of the pollen and filigree of corn, leads to solid abnormality occur, institute
It is pollinated the time limit with regulation.
Further, in step (1), big group's plantation is: northern production of hybrid seeds area plant photosensitive self-mating system 300~
500 plants.Population genetic offspring can be guaranteed for the selectable space of objective trait from amount in this way.
Further, in step (1), the individual plant selfing or strain cross are: in the self-mating system of plantation, looking for except plant height
The female tassel of the constant plant pair of economical character outside Ear height distinguishes bagging, male and female flowering is normally selfed, one side of male and female
It normally does strain cross, and listed son, records its pollination date, when harvest, retains the solid fruit ear up to 30 or more, point
It numbers and saves after fringe threshing, tame material as next year；The abnormal removal of female tassel does not have to bagging.Be conducive to look into this way
Source and data record are called in, workload is reduced.
Further, in step (1) and (2), south choosing north, which is tamed and dociled, to be specifically referred to select using Suwan germplasm in Southwest China
It educates photosensitive stable self-mating system and assembles cenospecies, which tames light sensitivity in northern production of hybrid seeds area.
Herein described QB48: growth is more healthy and stronger, and leaf color is denseer, and leaf margin is wavy more, and tassel branch is less and short, pollen
In amount, single fringe is larger, but cob is thicker, and seed is larger, and grain coloured gold is yellow, solids.Breeding time 112 days, 222 centimetres of plant height, fringe position
It is 90 centimetres high, 15 centimetres of spike length, 4.3 centimetres of fruit ear diameter, 12.8 row of tassel row number, every row grain number 25.6,312 grams of mass of 1000 kernel,
Fruit ear cartridge type, general combining ability higher (" breeding and application of good inbred lines QB48 ", Zhu Yunfang, Chen Zehui, Wang An
Expensive, Guo Xiangyang, Guizhou Agricultural Sciences, 2014-07-15).In acquisition Minister Agriculture of China Plant new variety protection authorization in 2010,
Kind power number are as follows: CNA20070788.4.What the cenospecies assembled was applied in production has golden single 999, bright list 008, precious jade 999
Deng more than 10.
Herein described T32: plant type is relatively hung down loosely, and blade is roomy, and leaf color is dark green, and breeding time 125 days or so, 238 lis of plant height
Rice, 98 centimetres of Ear height；16 centimetres of spike length, thick 4.6 centimetres of fringe, 14 row of tassel row number, row grain number 32,31.0 grams of 100-grain weight, out
Seed rate 85%.Tassel sterile lemma green, anther yellow, female Honoka green.The white axis of fruit ear, seed yellow, Hard grain type.Variable rate technology is green
Stalk is mature, anti-northern and southern leaf blight, rust etc..What the cenospecies assembled was applied in production has gold and jade 818, Guidan No.8 etc. multiple.
Herein described GD909: plant type half is compact, and blade is roomy, and leaf color is dark green, and breeding time 124 days or so, plant height 215
Centimetre, 87 centimetres of Ear height；16 centimetres of spike length, thick 4.5 centimetres of fringe, 14~16 row of tassel row number, row grain number 32,100-grain weight 29.0
Gram, seed-producing rate 85%.Tassel sterile lemma green, anther yellow, female Honoka green.The white axis of fruit ear, seed yellow, Hard grain type.Field
It is mature to show green stalk, anti-northern and southern leaf blight, rust etc..What the cenospecies assembled was applied in production has magnificent 908, your Zhuo Yu 9
Etc. multiple, currently, more units using the self-mating system as Testers, participate in the experiment and combine a batch, the stage is being highlighted.
Herein described XZ50612: seedling growth is strong, and leaf color is green, leaf sheath purple, leaf margin purple.Blade clean and jerk have item
Line, plant type half is compact, plant height 236cm, Ear height 93cm, and the complete stool number of blade 18, the stem later period is purple, plant neat one
It causes；Tassel primary branch 12 or so, the tassel long 30cm of the minimum above main shaft in side shoot position, the above main shaft in highest side shoot position is long
23cm, male flower sterile lemma are aubergine, and anther is aubergine, and female flower is red；Fruit ear is tubular, spike length 16cm, the thick 5.0cm of fringe,
16 row of tassel row number, mass of 1000 kernel 385g or so, seed yellow, Hard grain type, cob white, comprehensive disease resistance are strong.In Shen in 2018
Please Minister Agriculture of China's New species right in plant protection, accepted.What the cenospecies assembled was applied in production has Xing Nongdan 5, north
Jade 1521 etc. is multiple, currently, more units participate in the experiment using the self-mating system as Testers and combine a batch, highlighting the stage.
Herein described S611: seedling growth is stronger, and leaf color is dark green.Blade clean and jerk, plant type half is compact, plant height 166cm, fringe
The high 75cm in position, the complete stool number of blade 17, plant neat and consistent；Tassel primary branch is more, and fruit ear is tubular, spike length 14cm, and fringe is thick
4.1cm, 14 row of tassel row number, mass of 1000 kernel 292g or so, seed yellow, Hard grain type, cob white, comprehensive disease resistance are strong.It assembles
Cenospecies applied in production have Qiandan19, friend beautiful No. 8 etc. it is multiple.
Since present invention employs above technical schemes, have the advantages that
(1) the application is beautiful using the gold list 999 of Southwest China, Guidan No.8, gold and jade 818, gold and jade 908, Xing Nongdan 5, friend
The tropical and subtropical zone of No. 8 cenospecies photosensitive corn Suwan self-mating system QB48, T32, GD909, XZ50612, S611 are as domestication material
Material, is excellent in and general combining ability is good, using the method for " taming and dociling in south choosing north ", makes its light sensitivity insensitiveness, to these self-mating systems
The cenospecies assembled carries out seed production in the north, to hybrid seed yield and quality is improved, saves production cost and is of great significance,
It has filled up and has produced the blank that upper excellent Suwan self-mating system is directly tamed in the north.
(2) present invention is miscellaneous by using fixed sowing day, setting pollination time limit, the plantation of big group, individual plant selfing or strain
The method of friendship carries out " taming and dociling in south choosing north ", obtain reduction in the life period, male and female are coordinated, setting percentage is normal, light sensitivity insensitiveness, can be in the north
It is normally carried out the domestication self-mating system of seed production, improves the setting percentage after Suwan germplasm is tamed in north, setting percentage mentions
High by 42~53%, hybrid seed yield improves 51.3~187.6%, shortens breeding time, and flowering time has done sth. in advance 5~15d,
The efficiency of seed production is finally improved with the corn inbred line tamed, the Key technique problem of seed production is solved, is
It is laid a good foundation using the corn hybrid seed that tropical and subtropical zone Suwan germplasm breeding adapts to Southwestern China mountain area.
(3) present invention utilizes the photosensitive jade of southwestern cenospecies to solve the difficult technical problem of southwest seed production
Rice Suwan self-mating system belongs to domestication self-mating system as domestication material, using photosensitive material in southwestern multiple spot Breeding of Inbred Lines,
Northern production of hybrid seeds area S1 generation selection directly carries out light sensitivity domestication with mutual cross mode between individual plant selfing or strain, gives its domestication
Condition is selected, is identified that the hybrid seed of the Day neutral self-mating system that will be obtained after the domestication of the north, preparation is used for Southwest Mountainous Areas
It is planted in production, this method can retain the coordinate force and other economical characters of former self-mating system, and the Objective solved the problems, such as is clear.
(4) a collection of corn Suwan self-mating system to light sensitivity insensitiveness can be filtered out using the acclimation method of the application, it will
The Cross Combinations of its new breeding are produced in the northern production of hybrid seeds applied to Southwestern China, are solved the problems, such as in production with kind.
(5) cannot normally be blossomed and beared fruit due to photosensitive corn Suwan self-mating system in northern directly plantation, the application by
The method of north domestication, makes it in northern suitable growth, reaches to light sensitivity insensitiveness, and retaining, the other characters of former self-mating system are constant
In the case where, moreover it is possible to it solves the problems, such as that it blossoms and bears fruit, improves its yield.
(6) the application method is simple, easy to operate, reduces seed production cost, can protect after being identified by this method
Cenospecies and former cenospecies after card domestication is completely the same, is solving to also ensure the suitable of original cenospecies while seed production
Ying Xing.
(7) condition creativeness needed for the application combination corn growth selects scientific and reasonable place to tame, and combines
It tames place and corn growth rule Creative Design goes out to sow day, pollination time limit, implantation methods, avoid photosensitive corn inbred line
Cenospecies and situations such as inconsistent original cenospecies character, occur after the north is solid bad and domestication, reduce labor intensity,
Solves the problems, such as seed production.
Detailed description of the invention
In order to illustrate more clearly of present example or technical solution in the prior art, to embodiment or will show below
There is in technical description required attached drawing do simply to introduce, it should be apparent that, the accompanying drawings in the following description is only the present invention
Some examples to those skilled in the art, can also be attached according to these under the premise of not paying creativeness
Figure obtains other attached drawings:
Fig. 1 is the process flow chart that the application is passivated corn Suwan self-mating system light sensitivity.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited to these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
A method of passivation corn Suwan self-mating system light sensitivity, comprising the following steps:
(1) parent of biggish single 999 (QB48 × J106) of cenospecies gold of upper popularizing area is produced using Southwest China
QB48, self-mating system are itself to be excellent in and the photosensitive corn Suwan self-mating system of tropical and subtropical zone that general combining ability is good, therefore will
It is directly tamed as domestication material in northern production of hybrid seeds area, sows day, setting pollination time limit, the plantation of big group, list using fixing
The method of strain selfing or strain cross carries out " taming and dociling in south choosing north ", obtains 1 generation seed of domestication；
The direct domestication refers to using photosensitive corn Suwan self-mating system in north, allows it to carry out light sensitivity tame and docile
Change, not every the material blood relationship for closing any other source；The north production of hybrid seeds area refers to the west of a river that latitude is 37 °, height above sea level is 1300m
Corridor is as domestication place；The fixed sowing date is: sowing on April 19, light of the sowing date to corn during mature
Warm condition is conducive to achieve the purpose that light sensitive passivating material；The setting pollination time limit is: early August, after August 10th
What is bloomed will no longer pollinate；Big group's plantation is: photosensitive 300~500 plants of self-mating system of the plantation in northern production of hybrid seeds area；The list
Strain selfing or strain cross are: in the self-mating system of plantation, the plant pair for looking for the economical character in addition to plant height Ear height constant is female
Tassel distinguishes bagging, male and female flowering is normally selfed, one side of male and female normally does strain cross, and listed son, records it
Pollination date retains the solid fruit ear up to 30 or more when harvest, divides after fringe threshing to number and saves, and tames material as next year
Material；The abnormal removal of female tassel does not have to bagging；
(2) it the domestication 1 generation seed that step (1) obtains is carried out number after point fringe threshing saves seed to plant by head progeny row, often
A head progeny row plants 30 plants or more, is tamed according to " taming and dociling in south choosing north " method of step (1), compares upper 1 year pollination date,
The plant that every primary selection of domestication shifts to an earlier date 1~2d is selfed, and selection setting percentage retains up to 30% or more fruit ear when harvest, obtains
2 generation seeds must be tamed；
(3) the domestication 2 generation seed obtained in step (2) is continued into domestication 3 times according to the method for step (2), is tamed
Mostly for seed, it is screened out from it that male and female florescence shortens 5d and male and female are coordinated, setting percentage improves 42% domestication and stablizes kind
Son；Make male parent using the stable self-mating system of the domestication to be hybridized with J106, the self-mating system after the cenospecies assembled and domestication
Identify, be compared respectively with former cenospecies and self-mating system do not have discrepant domestication to stablize certainly by field trapping test and DNA
System is handed over, golden single 999 parent is left as, the stabilization seed of light sensitivity insensitiveness is obtained, can be used as male parent benefit in northern production of hybrid seeds area
Corn Suwan self-mating system.
Embodiment 2
A method of passivation corn Suwan self-mating system light sensitivity, comprising the following steps:
(1) Guidan No.8 (T32 × P159), the gold and jade 818 of the upper larger cenospecies of popularizing area are produced using Southwest China
The parent T32 of (T32 × QB506), self-mating system are itself to be excellent in and the photosensitive jade of tropical and subtropical zone that general combining ability is good
Rice Suwan self-mating system, therefore directly tamed as domestication material in northern production of hybrid seeds area, it is pollinated using fixed sowing day, setting
Time limit, the plantation of big group, the method progress of strain cross " taming and dociling in south choosing north ", acquisition 1 generation seed of domestication；
The direct domestication refers to using photosensitive corn Suwan self-mating system in north, allows it to carry out light sensitivity tame and docile
Change, not every the material blood relationship for closing any other source；The north production of hybrid seeds area refers to the west of a river that latitude is 39 °, height above sea level is 1600m
Corridor is as domestication place；The fixed sowing date is: sowing April 21, the sowing date is to during corn maturation
Light and temperature condition is conducive to achieve the purpose that light sensitive passivating material；The setting pollination time limit is: early August, August 10 days with
What is bloomed afterwards will no longer pollinate；Big group's plantation is: photosensitive 300~500 plants of self-mating system of the plantation in northern production of hybrid seeds area；It is described
Strain cross is: in the self-mating system of plantation, the female tassel difference of the plant pair for looking for the economical character in addition to plant height Ear height constant
The normal plant of one side of male and female is used for phase mutual cross between strain by bagging, and listed son, records its pollination date, and when harvest will
The solid fruit ear up to 30 or more retains, and numbers preservation after dividing fringe threshing, tames material as next year；Female tassel is abnormal
Removal without bagging handle；
(2) it the domestication 1 generation seed that step (1) obtains is carried out number after point fringe threshing saves seed to plant by head progeny row, often
A head progeny row plants 30 plants or more, is tamed according to " taming and dociling in south choosing north " method of step (1), compares upper 1 year pollination date,
The plant that every primary selection of domestication shifts to an earlier date 1~2d is selfed, and selection setting percentage retains up to 30% or more fruit ear when harvest, obtains
2 generation seeds must be tamed；
(3) the domestication 2 generation seed obtained in step (2) is continued into domestication 3 times according to the method for step (2), is tamed
Mostly for seed, it is screened out from it female fringe florescence shortening 5d, tassel florescence shortens 9d and male and female are coordinated, setting percentage improves
Seed is stablized in 51% domestication；The method of the identification are as follows: using the stable self-mating system of the domestication make male parent and P159 and
QB506 hybridizes respectively, and the self-mating system after the cenospecies assembled and domestication is identified by field trapping test and DNA, respectively with original
Cenospecies and self-mating system compare, and do not have discrepant domestication to stablize self-mating system, are left as the parent of Guidan No.8, gold and jade 818, obtain
It obtains the stabilization seed of light sensitivity insensitiveness, can be used as the corn Suwan self-mating system that male parent utilizes in northern production of hybrid seeds area.
Embodiment 3
A method of passivation corn Suwan self-mating system light sensitivity, comprising the following steps:
(1) parent of the upper biggish cenospecies gold and jade 908 (QR11 × GD909) of popularizing area is produced using Southwest Mountainous District in China
This GD909, self-mating system are itself to be excellent in and the photosensitive corn Suwan self-mating system of tropical and subtropical zone that general combining ability is good,
Therefore directly tamed as domestication material in northern production of hybrid seeds area, using fixed sowing day, setting pollination time limit, big group's kind
It plants, the method for individual plant selfing progress " taming and dociling in south choosing north ", acquisition 1 generation seed of domestication；
The direct domestication refers to using photosensitive corn Suwan self-mating system in north, allows it to carry out light sensitivity tame and docile
Change, not every the material blood relationship for closing any other source；The north production of hybrid seeds area refers to the west of a river that latitude is 37 °, height above sea level is 1400m
Corridor is as domestication place；The fixed sowing date is: sowing April 19, the sowing date is to during corn maturation
Light and temperature condition is conducive to achieve the purpose that light sensitive passivating material；The setting pollination time limit is: in mid or late July to August
In the first tenday period of a month, what is bloomed after August 10th will no longer pollinate；Big group's plantation is: planting photosensitive self-mating system in northern production of hybrid seeds area
300~500 plants；The individual plant selfing or strain cross are: in the self-mating system of plantation, looking for the agronomy in addition to plant height Ear height
The female tassel of the constant plant pair of shape distinguishes bagging, male and female flowering is normally selfed, one side of male and female normally does strain cross,
And listed son, record its pollination date, and when harvest, retains the solid fruit ear up to 30 or more, and divide after fringe threshing to number and saves,
Material is tamed as next year；The abnormal removal of female tassel does not have to bagging；
(2) it the domestication 1 generation seed that step (1) obtains is carried out number after point fringe threshing saves seed to plant by head progeny row, often
A head progeny row plants 30 plants or more, is tamed according to " taming and dociling in south choosing north " method of step (1), compares upper 1 year pollination date,
The plant that every primary selection of domestication shifts to an earlier date 1~2d is selfed, and selection setting percentage retains up to 30% or more fruit ear when harvest, obtains
2 generation seeds must be tamed；
(3) the domestication 2 generation seed obtained in step (2) is continued into domestication 2 times according to the method for step (2), is tamed
Mostly for seed, it is screened out from it female fringe florescence shortening 14d, tassel florescence shortens 15d and male and female are coordinated, setting percentage improves
Seed is stablized in 47% domestication；The method of the identification are as follows: make male parent using the stable self-mating system of the domestication and QR11 is carried out
Hybridize, the self-mating system after the cenospecies assembled and domestication is identified by field trapping test and DNA, respectively with former cenospecies and certainly
It hands over system to compare, does not have discrepant domestication to stablize self-mating system, be left as the parent of gold and jade 908, obtain the stabilization of light sensitivity insensitiveness
Seed, the corn Suwan self-mating system utilized in northern production of hybrid seeds area.According to two parent's own situations of cenospecies, corn Suwan
Self-mating system can be used as male parent or maternal utilization.
Embodiment 4
A method of passivation corn Suwan self-mating system light sensitivity, comprising the following steps:
(1) produce upper popularizing area biggish cenospecies Xing Nongdan's 5 (XZ9850 × XZ50612) using Southwest China
Parent XZ50612, self-mating system are itself to be excellent in and the photosensitive corn Suwan selfing of tropical and subtropical zone that general combining ability is good
System, therefore directly tamed as domestication material in northern production of hybrid seeds area, using fixed sowing day, setting pollination time limit, big group
Plantation, the method progress of individual plant selfing " taming and dociling in south choosing north ", acquisition 1 generation seed of domestication；
The direct domestication refers to using photosensitive corn Suwan self-mating system in north, allows it to carry out light sensitivity tame and docile
Change, not every the material blood relationship for closing any other source；The north production of hybrid seeds area refers to the west of a river that latitude is 39 °, height above sea level is 1500m
Corridor is as domestication place；The fixed sowing date is: sowing April 21, the sowing date is to during corn maturation
Light and temperature condition is conducive to achieve the purpose that light sensitive passivating material；The setting pollination time limit is: on late July to August
In ten days, what is bloomed after August 10th will no longer pollinate；Big group's plantation is: planting photosensitive self-mating system 300 in northern production of hybrid seeds area
~500 plants；The individual plant selfing is: in the self-mating system of plantation, the plant of looking for the economical character in addition to plant height Ear height constant
Bagging is distinguished to female tassel, male and female flowering is normally selfed, and listed son, records its pollination date, when harvest will be solid
Retain up to 30 or more fruit ears, number preservation after dividing fringe threshing, tames material as next year；Female tassel is abnormal to be gone
Except without bagging；
(2) it the domestication 1 generation seed that step (1) obtains is carried out number after point fringe threshing saves seed to plant by head progeny row, often
A head progeny row plants 30 plants or more, is tamed according to " taming and dociling in south choosing north " method of step (1), compares upper 1 year pollination date,
The plant that every primary selection of domestication shifts to an earlier date 1~2d is selfed, and selection setting percentage retains up to 30% or more fruit ear when harvest, obtains
2 generation seeds must be tamed；
(3) the domestication 2 generation seed obtained in step (2) is continued into domestication 2 times according to the method for step (2), is tamed
Mostly for seed, it is screened out from it female fringe florescence shortening 12d, tassel florescence shortens 13d and male and female are coordinated, setting percentage improves
Seed is stablized in 53% domestication；The method of the identification are as follows: using the stable self-mating system of the domestication make male parent and XZ9850 into
Row hybridization, the self-mating system after the cenospecies assembled and domestication identified by field trapping test and DNA, respectively with former cenospecies and
Self-mating system compares, and does not have discrepant domestication to stablize self-mating system, is left as Xing Nongdan 5 parents, obtains light sensitivity insensitiveness
The corn Suwan self-mating system stablizing seed, being utilized in northern production of hybrid seeds area.According to two parent's own situations of cenospecies, the corn
Suwan self-mating system can be used as male parent or maternal utilization.
Embodiment 5
A method of passivation corn Suwan self-mating system light sensitivity, comprising the following steps:
(1) parent of the biggish cenospecies friend of upper popularizing area beautiful No. 8 (QB1064 × S611) is produced using Southwest China
S611, self-mating system are itself to be excellent in and the photosensitive corn Suwan self-mating system of tropical and subtropical zone that general combining ability is good, therefore will
It is directly tamed as domestication material in northern production of hybrid seeds area, sows day, setting pollination time limit, the plantation of big group, list using fixing
The method of strain selfing or strain cross carries out " taming and dociling in south choosing north ", obtains 1 generation seed of domestication；
The direct domestication refers to using photosensitive corn Suwan self-mating system in north, allows it to carry out light sensitivity tame and docile
Change, not every the material blood relationship for closing any other source；The north production of hybrid seeds area refers to the west of a river that latitude is 38 °, height above sea level is 1450m
Corridor is as domestication place；The fixed sowing date is: sowing April 20, the sowing date is to during corn maturation
Light and temperature condition is conducive to achieve the purpose that light sensitive passivating material；The setting pollination time limit is: in early August, August 10th
What is bloomed later will no longer pollinate；Big group's plantation is: photosensitive 300~500 plants of self-mating system of the plantation in northern production of hybrid seeds area；Institute
It states individual plant selfing or strain cross is: in the self-mating system of plantation, the plant of looking for the economical character in addition to plant height Ear height constant
Bagging is distinguished to female tassel, male and female flowering is normally selfed, one side of male and female normally does strain cross, and listed son, note
Record its pollination date, when harvest retains the solid fruit ear up to 30 or more, divides after fringe threshing to number and saves, and tames and dociles as next year
Change material；The abnormal removal of female tassel does not have to bagging；
(2) it the domestication 1 generation seed that step (1) obtains is carried out number after point fringe threshing saves seed to plant by head progeny row, often
A head progeny row plants 30 plants or more, is tamed according to " taming and dociling in south choosing north " method of step (1), compares upper 1 year pollination date,
The plant that every primary selection of domestication shifts to an earlier date 1~2d is selfed, and selection setting percentage retains up to 30% or more fruit ear when harvest, obtains
2 generation seeds must be tamed；
(3) the domestication 2 generation seed obtained in step (2) is continued into domestication 3 times according to the method for step (2), is tamed
Mostly for seed, it is screened out from it female fringe florescence shortening 11d, tassel florescence shortens 15d and male and female are coordinated, setting percentage improves
Seed is stablized in 47% domestication；The method of the identification are as follows: using the stable self-mating system of the domestication make male parent and QB1064 into
Row hybridization, the self-mating system after the cenospecies assembled and domestication identified by field trapping test and DNA, respectively with former cenospecies and
Self-mating system compares, and does not have discrepant domestication to stablize self-mating system, is left as friendly beautiful No. 8 parents, obtains the steady of light sensitivity insensitiveness
Determine seed, can be used as the corn Suwan self-mating system that male parent utilizes in northern production of hybrid seeds area.
The technical effect can be reached in order to further illustrate the present invention, do following experiment:
One, the materials and methods of DNA identification
1. material
Select the domestication material obtained after the embodiment of the present application 1-5 domestication, the domestication material selection 3 of each embodiment
Part, the Day neutral corn Suwan self-mating system after domestication is subjected to DNA fingerprinting with former photosensitive self-mating system and is compared, it is selected
Material is as shown in table 1 below.
Table 1
2.DNA identification method
Referring to the phase of China's agricultural industry criteria NY/T1432-2014 " corn variety identification technology regulation-SSR marker method "
Regulation is closed, the material selected in above-mentioned table 1 is subjected to 20 taproot SSR Primers and distribution detects, main test job
Program: each material carries out PCR polymorphism amplification → capillary electrophoresis detection of the extraction → 20 pair taproot SSR primer of DNA
→ spectral band analysis → statistical analysis.20 pairs of taproot SSR Primers used in table 1 and distribution see the table below 2.
2 20 pairs of taproot SSR Primers of table and distribution
3. qualification result
SSR amplified production is pressed has band to be denoted as " 1 " on identical migration position (identical molecular weight fragment), and no band is denoted as " 0 ",
Database is all established with 1,0 statistics.PCR amplification is carried out with 20 parts of materials of above-mentioned 20 couples of taproot SSR primer pair, it will be each pair of
The amplification bands of a spectrum of primer combine, and the comparison result of the identical storeroom in corn Suwan self-mating system domestication front and back see the table below 3,
Difference number of sites is 0-3.
The analysis result of the identical storeroom in 3 corn Suwan self-mating system of table domestication front and back
| Serial number | Variety name | Differential primer logarithm | Serial number | Variety name | Differential primer logarithm |
| 1 | QB48 | 11 | GD909-2 | 1 | |
| 2 | QB48-1 | 1 | 12 | GD909-3 | 0 |
| 3 | QB48-2 | 0 | 13 | XZ50612 | |
| 4 | QB48-3 | 2 | 14 | XZ50612-1 | 0 |
| 5 | T32 | 15 | XZ50612-2 | 2 | |
| 6 | T32-1 | 0 | 16 | XZ50612-3 | 1 |
| 7 | T32-2 | 1 | 17 | S611 | |
| 8 | T32-3 | 2 | 18 | S611-1 | 3 |
| 9 | GD909 | 19 | S611-2 | 0 | |
| 10 | GD909-1 | 3 | 20 | S611-3 | 2 |
Two, investigation experiment
2018, inventor planted 1~5 method domestication through the embodiment of the present invention in Zhangye City, Gansu Province respectively
Suwan self-mating system, at the same using without domestication the photosensitive corn Suwan self-mating system QB48, T32 of tropical and subtropical zone, GD909,
As a control group, investigation Suwan self-mating system tames florescence, setting percentage and the hybrid seed yield after preceding and domestication by XZ50612, S611
Situation, every group carries out 3 parallel laboratory tests respectively, is averaged as experimental result, it is as shown in table 4 to investigate experimental result.
Table 4
Note: in table 4, refer to the number of days of florescence ahead of time ahead of time；It is the light that the domestication of 1 method of embodiment obtains after QB48 domestication
The corn Suwan self-mating system of quick property insensitiveness is the corn for the light sensitivity insensitiveness that the domestication of 2 method of embodiment obtains after T32 domestication
Suwan self-mating system is the corn Suwan self-mating system for the light sensitivity insensitiveness that the domestication of 3 method of embodiment obtains after GD909 domestication,
It is the corn Suwan self-mating system for the light sensitivity insensitiveness that the domestication of 4 method of embodiment obtains after XZ50612 domestication, is after S611 domestication
The corn Suwan self-mating system for the light sensitivity insensitiveness that the domestication of 5 method of embodiment obtains.
By 4 experimental data of table it is found that being counted according to strain number 60% of blooming, the florescence of the Suwan self-mating system before domestication is in August
It blooms within 7th~15, for setting percentage 11~51%, hybrid seed yield is 89~213.5kg；And it is tamed by the application method
Afterwards, the florescence of Suwan self-mating system blooms for 25 days~August 7th in July, and florescence has done sth. in advance 5~15d, setting percentage 61~
98%, setting percentage improves 42~53%, and hybrid seed yield is 256~326kg, and hybrid seed yield improves 51.3~187.6%.
Three, application example
1, summer in 2018, inventor spread (sub- heat Guangxi Zhuang Autonomous Region Nanning City (torrid zone), Lincang City, Yunnan Province are brave
Band) and Guizhou Province Bijie City (temperate zone) three different ecological points, using completely random aligning method, according to 4500 plants per acre progress
Plantation, April 15, three points were sowed simultaneously, carried out the corn that field trapping test Examples 1 to 3 method is tamed respectively
Suwan self-mating system and tropical and subtropical zone photosensitive self-mating system QB48, T32, GD909 without domestication, every sample seed carry out three respectively
Secondary to repeat to test, plot area is 20m2, their light sensitivity is investigated respectively, records its plant height, florescence, solid
The characters such as rate and cell production, test result are as shown in table 5 below.
Table 5
Note: being the corn Suwan selfing for the light sensitivity insensitiveness that the domestication of 1 method of embodiment obtains in table 5, after QB48 domestication
System is the corn Suwan self-mating system for the light sensitivity insensitiveness that the domestication of 2 method of embodiment obtains after T32 domestication, is after GD909 domestication
The corn Suwan self-mating system for the light sensitivity insensitiveness that the domestication of 3 method of embodiment obtains.
By 5 experimental data of table it is found that before taming corn Suwan self-mating system QB48, T32, GD909 in south China the torrid zone,
Subtropical zone and three, temperate zone ecotope are planted, increasingly length, setting percentage of higher and higher, breeding time from the torrid zone to temperate zone plant height
Lower and lower, variation is obvious, but yield does not almost change, as photosensitive self-mating system；And pass through 1~3 side of the embodiment of the present application
The corn Suwan self-mating system that method is tamed is reacted without apparent light sensitivity under three kinds of different ecological environment, and passes through this
Application method reduces plant height by 29~77.5cm, and florescence does sth. in advance 4~14d, setting percentage and itself yield have small raising trend,
Without significant change, illustrates to tame light sensitivity in the north to photosensitive corn Suwan self-mating system, return to south and represent planted in different ecological areas kind
It plants unchanged to its own yield.It can thus be appreciated that: the method for the present invention is tamed and dociled to photosensitive corn Suwan self-mating system light sensitivity in the north
Change effectively, the corn Suwan self-mating system tamed is to Day neutral.
In conclusion the application is using the gold list 999 of Southwest China, Guidan No.8, gold and jade 818, magnificent 908, Xing Nongdan 5
Number, tropical and subtropical zone photosensitive corn Suwan self-mating system QB48, T32, GD909, XZ50612, S611 of beautiful No. 8 cenospecies of friend make
To tame material, it is excellent in and general combining ability is good, by using fixed sowing day, setting pollination time limit, big group's kind
Plant, the method for individual plant selfing or strain cross carries out " taming and dociling in south choosing north ", making can be in north after corn Suwan self-mating system light sensitivity insensitiveness
Side is normally carried out seed production, improves 42~53% in the setting percentage of north after taming Suwan germplasm, hybrid seed yield
51.3~187.6% are improved, breeding time is shortened, flowering time has done sth. in advance 5~15d, to improving hybrid seed yield and quality,
It saves production cost to be of great significance, has filled up and produced the blank that upper excellent Suwan self-mating system is directly tamed in the north, solved
The Key technique problem of seed production, for the corn for adapting to Southwestern China mountain area using tropical and subtropical zone Suwan germplasm breeding
Cenospecies is laid a good foundation.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, nothing
By from the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended
Claim rather than above description limit, it is intended that by the institute in the meaning and scope for the equivalent loins for falling in claim
It changes and includes within protection scope of the present invention.
Claims (10)
1. a kind of method for being passivated corn Suwan self-mating system light sensitivity, which comprises the following steps:
(1) it is directly tamed and dociled as domestication material in northern production of hybrid seeds area using the photosensitive corn Suwan self-mating system of Southwest China cenospecies
Change, " Nan Xuanbei is carried out using the method for fixed sowing day, setting pollination time limit, the plantation of big group, individual plant selfing or strain cross
Tame and docile ", obtain 1 generation seed of domestication；
(2) it the domestication 1 generation seed that step (1) obtains is carried out number after point fringe threshing saves seed to plant by head progeny row, Mei Gesui
30 plants of row plantation or more is tamed according to " taming and dociling in south choosing north " method of step (1), compares upper 1 year pollination date, often tame and docile
Change the plant that primary selection shifts to an earlier date 1~2d to be selfed, selection setting percentage retains up to 30% or more fruit ear when harvest, is tamed and dociled
Change 2 generation seeds；
(3) the domestication 2 generation seed obtained in step (2) is continued domestication 2~3 times according to the method for step (2), it is more obtains domestication
For seed, the domestication for being screened out from it reduction in the life period and male and female coordination stablizes seed, which is stablized seed and is identified,
And leave light sensitivity insensitiveness and setting percentage normally tames the parent for stablizing seed as former cenospecies, it obtains for production and right
The corn Suwan self-mating system of Day neutral；
In step (3), the method for the identification are as follows: it is corresponding with domestication material to make male parent using the stable self-mating system of the domestication
Maternal self-mating system is hybridized, and the self-mating system after the cenospecies assembled and domestication passes through field kind in Southwestern China different ecological point
Plant identification and DNA identification, compared and compared with former cenospecies and self-mating system, in addition to light sensitivity, other characters and former cenospecies and
Self-mating system does not have discrepant domestication to stablize self-mating system, is left as the parent of former cenospecies.
2. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), the direct domestication refers to using photosensitive corn Suwan self-mating system in north, it is allowed to tame light sensitivity,
Not every the material blood relationship for closing any other source.
3. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), the photosensitive corn Suwan self-mating system refer to sub- in the torrid zone that Southwest China itself is excellent in and general combining ability is good
The torrid zone photosensitive corn Suwan self-mating system QB48, T32, GD909, XZ50612 and S611.
4. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), the cenospecies refer to that planting its yielding ability, yield stability, adaptability in Southwest Mountainous District in China shows excellent gold list
999, Guidan No.8, magnificent 818, gold and jade No. 5 cenospecies of 908 and Xing Nongdan.
5. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), the north production of hybrid seeds area refer to that the Hexi Corridor that latitude is 37~39 °, height above sea level is 1300~1600m is used as domestication ground
Point.
6. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), the fixed sowing date is: being sowed within 19th~21 in April, light temperature of the sowing date to corn during mature
Condition is conducive to achieve the purpose that light sensitive passivating material.
7. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), the setting pollination time limit is: according to the breeding time of self-mating system, by pollination date in mid-July to early August, August
What is bloomed after 10 days will no longer pollinate.
8. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), big group's plantation is: photosensitive 300~500 plants of self-mating system of the plantation in northern production of hybrid seeds area.
9. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1), the individual plant selfing or strain cross are: in the self-mating system of plantation, looking for economical character in addition to plant height Ear height not
The female tassel of the plant pair of change distinguishes bagging, male and female flowering is normally selfed, one side of male and female normally does strain cross, and hangs
Brand records its pollination date, and when harvest retains the solid fruit ear up to 30 or more, divides after fringe threshing to number and saves, as
Next year tames material；The abnormal removal of female tassel does not have to bagging.
10. a kind of method for being passivated corn Suwan self-mating system light sensitivity according to claim 1, it is characterised in that: in step
Suddenly (1) and (2), south choosing north, which is tamed and dociled, to be specifically referred to using Suwan germplasm in the photosensitive stable selfing of Southwest China breeding
System assembles cenospecies, which tames light sensitivity in northern production of hybrid seeds area.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719126A (en) * | 2015-03-09 | 2015-06-24 | 云南省农业科学院粮食作物研究所 | Method for improving photoperiod sensitivity of tropical maize germplasm |
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2019
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| CN104719126A (en) * | 2015-03-09 | 2015-06-24 | 云南省农业科学院粮食作物研究所 | Method for improving photoperiod sensitivity of tropical maize germplasm |
| CN107041299A (en) * | 2017-05-26 | 2017-08-15 | 北京市农林科学院 | A kind of method of the Rapid deactivation torrid zone/Subtropical Corn Germplasms light sensitivity |
| CN107864851A (en) * | 2017-11-09 | 2018-04-03 | 贵州省旱粮研究所 | A kind of method for solving the photosensitive self-mating system of Southwest Mountainous Areas corn |
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