CN107032867A - A kind of composite biological fertilizer feed additives containing fistulainA - Google Patents
A kind of composite biological fertilizer feed additives containing fistulainA Download PDFInfo
- Publication number
- CN107032867A CN107032867A CN201611022496.8A CN201611022496A CN107032867A CN 107032867 A CN107032867 A CN 107032867A CN 201611022496 A CN201611022496 A CN 201611022496A CN 107032867 A CN107032867 A CN 107032867A
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- CN
- China
- Prior art keywords
- fistulaina
- active compound
- feed additives
- salinomycin
- composite biological
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The present invention prepares fertilizer with the mixed liquor of microbial fermentation solution and active compound, and wherein active compound includes salinomycin and FistulainA, there is preferable application effect to plant, and environmentally safe, and application process is easy, and consumption is few.Experiment shows salinomycin with FistulainA while certain proportion can promote plant growth, plant disease-resistant ability can also be improved, increases the supply of plant nutrient, the growth of crop is stimulated, suppress harmful microbe activity, there is certain effect of increasing production to crop.
Description
Technical field
The present invention relates to a kind of bio-feritlizer, specifically a kind of composite biological fertilizer feed additives containing fistulainA,
Belong to biomass efficient and utilize technical field.
Background technology
In recent years, the administration of chemical fertilizer serves key effect to agricultural production and income.However, due to chronic administration
Fertilizer is learned, utilization rate of fertilizer declines, and organic fertilizer is insufficient, causes farmland ecological environment, soil physical and chemical property and soil micro-
Biota receives different degrees of destruction, and the quality of agricultural product is have impact on to a certain extent.The current soil resource of China
Present situation by artificial measure in the urgent need to supplementing the soil organism, it is ensured that agricultural planting level and crop product quantity are carried
It is high.This can promote the growth of crop and improve product quality just to environmentally friendly, material circulation to soil ecosystem,
The microbial manure that energy flow has special role brings good opportunity to develop.
China's microbial manure experienced the evolution of repeated rises and falls, existing decades history, can trace back to earliest
In the 1930s, being by the rihizobium japonicum of the soil microbiology expert professor Zhang Xianwu development of Chinese Famous, by 60 years
" 5406 " antibiotic bacteria fertilizer in generation and fixed nitrogen blue-green alge fertilizer, the VA mycorhiza of 70~the mid-80, the joint of 80~nineties are consolidated
Nitrogen bacterial manure, silicate bacteria preparation, photosynthetic bacteria microbial inoculum, PGPR preparations and organic fertilizer (stalk) decomposing agent etc..But there is also deficiency, very
In terms of many microbial manure basic research and application study only reside within Reason, strain isolation and field experiment,
Microbial manure industry integral level is not still high, many manufacturer production technological innovations not enough, be simply equipped, technique imperfection,
Welding, effect of fertilizer are unstable, and these problems all constrain the further development of microbial manure.Hypatulins A
And B, Meroterpenes from Hypericum patulum, Org.Lett, 2016,18 (20), 5360-5363.
It is the brand-new framework compound separated from hypericum.
Salinomycin is monocarboxylic acid polyethers animal specific antibiotic, is had to most of gram-positive bacterias and various coccidias
Stronger suppression and killing action, are not likely to produce drug resistance and cross-resistance, and excretion is rapid, and residual quantity is extremely low, can for pig
Prevent and treat diarrhoea, growth promotion, improve survival rate, be mainly used in the anti-coccidia American researcher of poultry and find that one kind " can be aimed at " simultaneously
The compound of cancer stem cell is killed, during laboratory research, it kills the effect of breast carcinoma stem cell with mouse than general
Logical cancer therapy drug Taxol (Taxol) is high 100 times.Salinomycin can not only kill the breast carcinoma stem cell with mouse, moreover it is possible to press down
They bear new tumour cell to system, while can also slow down the speed of growth of the tumour existed.
It has been found that salinomycin is mixed with FistulainA under certain proportion with microbial fermentation solution, it is possible to increase
Bio-feritlizer activity, promotes plant biological growth, improves plant disease-resistant ability.
The content of the invention
In view of the above-mentioned problems of the prior art, the present invention provides a kind of composite bio-fertilizer containing fistulainA
Additive, it is environmentally safe, and there is good disease resisting effect for plant.
To achieve these goals, the technical solution adopted by the present invention is：A kind of compound bio containing fistulainA
Fertilizer additive, includes the mixed liquor of microbial fermentation solution and active compound, and described active compound includes
FistulainA, described fistulainA structural formulas are as follows：
Described active compound also includes salinomycin, and described salinomycin and FistulainA mass ratio are in 1-20:
1,
The microbial fermentation solution is the zymotic fluid of Bacillus subtillis and micrococcus luteus.
The mass ratio of microbial fermentation solution and active compound is in 20-30：1.
Bacillus subtillis and micrococcus luteus are individually fermented, then obtained zymotic fluid is mixed.
The present invention prepares fertilizer with the mixed liquor of microbial fermentation solution and active compound, and technique is simple, with short production cycle,
Not only there is preferable application effect to plant, and it is environmentally safe, and application process is easy, and consumption is few.Experiment shows that salt is mould
Element mixes with the zymotic fluid of Bacillus subtillis and micrococcus luteus in certain proportion with FistulainA and plant can be promoted to give birth to
While long, moreover it is possible to improve plant disease-resistant ability, increase the supply of plant nutrient, stimulate the growth of crop, suppress to have
The activity of evil microorganism, there is certain effect of increasing production to crop.
Embodiment
Bacillus subtillis CICC10002 in text is purchased from China General Microbiological culture presevation administrative center, and gamboge is micro-
Coccus CICC 10269, purchased from China General Microbiological culture presevation administrative center.
Compound fistulainA involved in the present invention preparation method is referring to document:Fistulains A and B,New
Bischromones from the Bark of Cassia fistula, and Their Activities, Org.Lett.,
2015,17(11),pp 2638–2641。
Salinomycin, CAS 53003-10-4.
The fermentation condition for preparing Bacillus subtillis zymotic fluid is as follows：
Fermentation medium：Peptone 50g, beef leaching thing 30g, NaCl50g, agar 150g, distilled water 10L,
Condition of culture：PH7.0~7.5 temperature, 30~31 DEG C of 30~36h of time.
The fermentation condition of M. luteus fermented liquid is as follows：
Fermentation medium：Peptone 5.0g, beef leaching thing 3.0g, NaCl 5.0g, agar 15.0g, distilled water 1.0L,
pH7.0.[note] adds 5mg MnSO4H2O when cultivating bacillus.
Condition of culture：PH7.0~7.5 temperature, 30~32 DEG C of 24~28h of time.
Embodiment 1：
According to Mass Calculation, 15 parts of Bacillus subtillis zymotic fluid, 5 parts of M. luteus fermented liquid, active compound 1
Part, wherein active compound salinomycin and FistulainA mass ratio are 20:1, the environment is neutral environment, i.e. ph is
7。
Embodiment 2：
According to Mass Calculation, 15 parts of Bacillus subtillis zymotic fluid, 10 parts of M. luteus fermented liquid, active compound 1
Part, wherein active compound salinomycin and FistulainA mass ratio are 15:1.
Embodiment 3：
According to Mass Calculation, 15 parts of Bacillus subtillis zymotic fluid, 15 parts of M. luteus fermented liquid, active compound 1
Part, wherein active compound salinomycin and FistulainA mass ratio are 13:1.
Contrast test：
Experiment sets test group, and the fertilizer of addition is respectively：Using 1 group of embodiment as preparation standard, i.e., according to Mass Calculation,
15 parts of Bacillus subtillis zymotic fluid, 5 parts of M. luteus fermented liquid, 1 part of active compound, prepare different salinomycins with
The additive of FistulainA mass ratio, the additive prepared adds the ratio of 300ml additives according to 1000g turfs
Example prepares bio-feritlizer, and blank group does not contain salinomycin and FistulainA.
Using the method for feeding block seedlings raising, each nutritive cube fills soil 300g, transplanted during feeding block seedlings raising to 2~3 true leaves
Into the basin alms bowl for filling 5kg soil, per the potted plant young plant of kind 3, every group of 21 basin (repetition).Rate of fertilizer is 0.5% (quality in basin
Fraction)
Biomass is determined:After transplanting 2 months, each group young plant of random acquisition 6 determines plant height, overground part and underground
Fresh and dried quality in portion etc.
(dry mass assay method:105 DEG C of fixing 30min, 75 DEG C of drying to permanent quality).
Plant incidence:After tomato is transplanted 2 months, the incidence of droop is detected, the incidence of disease is calculated.
A is salinomycin, and B is fistulainA,
| Active compound | The incidence of disease (%) |
| A：B(20：1) | 55.7 |
| A：B(15：1) | 61.3 |
| A：B(12：1) | 8.7 |
| A：B(10：1) | 9.8 |
| A：B(5：1) | 60.9 |
| A：B(2：1) | 58.2 |
| A：B(1：1) | 65.7 |
| A：B(1：2) | 77.8 |
| A：B(1：10) | 75.1 |
| A | 50.6 |
| B | 72.3 |
| Blank group | 81.4 |
As a result show, except 10:1 and 12:Outside 1 two groups, the incidences of disease of other group group fertilizer plant be more than 50%,
Prevention effect is poor, and applies 10:1 and 12:1 group of the fertilizer plant droop incidence of disease is below 10%, and prevention effect is at least up to
To 90%.Active component is single salinomycin or FistulainA, and prevention effect is general, and wherein salinomycin is prevented with certain
Ability is controlled, but also there was only 50%, when the salinomycin and FistulainA mass ratio in active compound are in 10-12:When 1, fertilizer
Material can significantly reduce the incidence of disease, and synergy significantly, has preferable preventive and therapeutic effect to tomato droop.
| Active compound | Individual plant height |
| A：B(20：1) | 81.6 |
| A：B(15：1) | 84.7 |
| A：B(12：1) | 97.8 |
| A：B(10：1) | 97.1 |
| A：B(5：1) | 83.1 |
| A：B(2：1) | 85.6 |
| A：B(1：1) | 79.3 |
| A：B(1：2) | 77.9 |
| A：B(1：10) | 76.2 |
| A | 83.2 |
| B | 75.9 |
| Blank | 71.3 |
Results from pot experiment test shows, 10:1 and 12:The tomato plant growth of 1 two groups of processing is best, with other ratio groups
Compare, there is obvious difference, illustrate salinomycin in active compound and FistulainA mass ratio in 10-12:1 fertilizer
There are obvious growth-promoting functions to the growth of tomato plant.
Claims (4)
1. a kind of composite biological fertilizer feed additives containing fistulainA, it is characterised in that including microbial fermentation solution and work
Property composition mixed liquor, described active compound includes fistulainA, and described fistulainA structural formulas are as follows：
。
2. the composite biological fertilizer feed additives according to claim 1 containing fistulainA, it is characterised in that described
Active compound also includes salinomycin, and described salinomycin and FistulainA mass ratio are in 1-20:1
Composite biological fertilizer feed additives according to claim 1 containing fistulainA, it is characterised in that micro- life
Thing zymotic fluid is the zymotic fluid of Bacillus subtillis and micrococcus luteus.
3. a kind of composite biological fertilizer feed additives containing fistulainA according to claim 3, it is characterised in that micro-
The mass ratio of bio-fermented liquid and active compound is in 20-30：1.
4. the composite biological fertilizer feed additives according to claim 3 containing fistulainA, it is characterised in that withered grass bud
Born of the same parents bacillus and micrococcus luteus are individually fermented, then obtained zymotic fluid is mixed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611022496.8A CN107032867A (en) | 2016-11-16 | 2016-11-16 | A kind of composite biological fertilizer feed additives containing fistulainA |
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| CN201611022496.8A CN107032867A (en) | 2016-11-16 | 2016-11-16 | A kind of composite biological fertilizer feed additives containing fistulainA |
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| CN107032867A true CN107032867A (en) | 2017-08-11 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105294720A (en) * | 2015-05-08 | 2016-02-03 | 云南民族大学 | Dimerization chromone alkaloid compound as well as preparation method and application thereof |
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- 2016-11-16 CN CN201611022496.8A patent/CN107032867A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105294720A (en) * | 2015-05-08 | 2016-02-03 | 云南民族大学 | Dimerization chromone alkaloid compound as well as preparation method and application thereof |
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Application publication date: 20170811 |