CN107003508A - 选择性平面照明显微镜学仪器 - Google Patents
选择性平面照明显微镜学仪器 Download PDFInfo
- Publication number
- CN107003508A CN107003508A CN201580070405.5A CN201580070405A CN107003508A CN 107003508 A CN107003508 A CN 107003508A CN 201580070405 A CN201580070405 A CN 201580070405A CN 107003508 A CN107003508 A CN 107003508A
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- CN
- China
- Prior art keywords
- illumination
- objective lens
- sample
- light
- object lens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000005286 illumination Methods 0.000 title claims abstract description 66
- 238000000386 microscopy Methods 0.000 title claims description 17
- 230000003287 optical effect Effects 0.000 claims abstract description 60
- 230000003459 anti-dromic effect Effects 0.000 claims abstract description 8
- 230000000644 propagated effect Effects 0.000 claims abstract description 7
- 238000005516 engineering process Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 3
- 230000001427 coherent effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 238000003384 imaging method Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- 230000006978 adaptation Effects 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/086—Condensers for transillumination only
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0088—Inverse microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
Claims (10)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462095959P | 2014-12-23 | 2014-12-23 | |
US62/095959 | 2014-12-23 | ||
US201562109245P | 2015-01-29 | 2015-01-29 | |
US62/109245 | 2015-01-29 | ||
PCT/US2015/064512 WO2016105934A2 (en) | 2014-12-23 | 2015-12-08 | Selective plane illumination microscopy instruments |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107003508A true CN107003508A (zh) | 2017-08-01 |
CN107003508B CN107003508B (zh) | 2020-07-10 |
Family
ID=56151624
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201580070405.5A Active CN107003508B (zh) | 2014-12-23 | 2015-12-08 | 选择性平面照明显微镜学仪器 |
Country Status (5)
Country | Link |
---|---|
US (1) | US10481375B2 (zh) |
EP (1) | EP3237949A4 (zh) |
JP (1) | JP6671725B2 (zh) |
CN (1) | CN107003508B (zh) |
WO (1) | WO2016105934A2 (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109765684A (zh) * | 2019-03-03 | 2019-05-17 | 北京工业大学 | 多光路多方位实时显微成像系统 |
CN111602080A (zh) * | 2017-12-07 | 2020-08-28 | 欧洲分子生物学实验室 | 用于成像多个样品的样品保持器 |
CN114787684A (zh) * | 2019-11-13 | 2022-07-22 | 华盛顿大学 | 具有照明及收集物镜的非正交布置的开顶式光片显微镜 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017035078A1 (en) * | 2015-08-26 | 2017-03-02 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Systems and methods for multi-view nonlinear optical imaging for improved signal-to-noise ratio and resolution in point scanning multi-photon microscopy |
DE102016120683A1 (de) * | 2016-10-28 | 2018-05-03 | Carl Zeiss Microscopy Gmbh | Lichtblattmikroskop |
DE102017204325A1 (de) | 2017-03-15 | 2018-09-20 | Carl Zeiss Microscopy Gmbh | Anordnung, Mikroskop und Verfahren zur TIRF-Mikroskopie |
DE102017214189A1 (de) * | 2017-08-15 | 2019-02-21 | Carl Zeiss Microscopy Gmbh | Verfahren zum Betrieb einer Mikroskopieranordnung und Mikroskopieranordnung mit einem ersten Mikroskop und mindestens einem weiteren Mikroskop |
JP6876576B2 (ja) * | 2017-08-17 | 2021-05-26 | 日本電子株式会社 | 三次元像構築方法 |
GB201718587D0 (en) * | 2017-11-10 | 2017-12-27 | Luxendo Gmbh | Microscope stage |
WO2019148008A1 (en) * | 2018-01-26 | 2019-08-01 | University Of Washington | Apparatuses and methods for multi-direction digital scanned light sheet microscopy |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5570228A (en) * | 1991-04-19 | 1996-10-29 | Edge Scientific Instrument Company Llc | Fiber optic illumination system and method for a high definition light microscope |
CN101419335A (zh) * | 2007-10-22 | 2009-04-29 | 泰肯贸易股份公司 | 用于荧光测量的激光扫描装置 |
US20110115895A1 (en) * | 2008-07-30 | 2011-05-19 | Jan Huisken | Multidirectional selective plane illumination microscopy |
US20120098949A1 (en) * | 2010-10-22 | 2012-04-26 | Leica Microsystems Cms Gmbh | Spim microscope with a sted light sheet |
CN103176268A (zh) * | 2011-12-21 | 2013-06-26 | 佳能株式会社 | 显微镜 |
CN104054014A (zh) * | 2011-10-28 | 2014-09-17 | 莱卡微系统Cms有限责任公司 | 用于以spim显微术照明样品的布置 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AT353497B (de) | 1972-05-23 | 1979-11-12 | Leitz Ernst Gmbh | Vorrichtung an mikroskopen, zum automatischen fokussieren des geraetes auf unterschiedliche objekt-ebenen |
US5305139A (en) * | 1991-04-19 | 1994-04-19 | Unimat (Usa) Corporation, Formerly United Holdings, Inc. | Illumination system and method for a high definition 3-D light microscope |
US5774224A (en) * | 1997-01-24 | 1998-06-30 | International Business Machines Corporation | Linear-scanning, oblique-viewing optical apparatus |
US8254020B2 (en) * | 2006-04-20 | 2012-08-28 | Washington University | Objective-coupled selective plane illumination microscopy |
DE102011000835C5 (de) * | 2011-02-21 | 2019-08-22 | Leica Microsystems Cms Gmbh | Abtastmikroskop und Verfahren zur lichtmikroskopischen Abbildung eines Objektes |
WO2012122027A2 (en) | 2011-03-04 | 2012-09-13 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Optomechanical module for converting a microscope to provide selective plane illumination microscopy |
DE102011051042B4 (de) | 2011-06-14 | 2016-04-28 | Leica Microsystems Cms Gmbh | Abtastmikroskop und Verfahren zur lichtmikroskopischen Abbildung eines Objektes |
DE102012110077A1 (de) | 2012-10-23 | 2014-06-26 | Karlsruher Institut für Technologie | Mikroskop mit mindestens einem Beleuchtungsstrahl in Form einer Lichtscheibe |
US9874736B2 (en) * | 2013-04-29 | 2018-01-23 | The Regents Of The University Of California | Apparatus and method for an inclined single plane imaging microscope box (iSPIM box) |
WO2016049368A1 (en) * | 2014-09-24 | 2016-03-31 | The United States Of America, As Represented By Secretary, Department Of Health And Human Services | Resolution enhancement for light sheet microscopy systems and methods |
-
2015
- 2015-12-08 EP EP15874101.7A patent/EP3237949A4/en active Pending
- 2015-12-08 JP JP2017533907A patent/JP6671725B2/ja active Active
- 2015-12-08 CN CN201580070405.5A patent/CN107003508B/zh active Active
- 2015-12-08 WO PCT/US2015/064512 patent/WO2016105934A2/en active Application Filing
- 2015-12-08 US US15/536,490 patent/US10481375B2/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5570228A (en) * | 1991-04-19 | 1996-10-29 | Edge Scientific Instrument Company Llc | Fiber optic illumination system and method for a high definition light microscope |
CN101419335A (zh) * | 2007-10-22 | 2009-04-29 | 泰肯贸易股份公司 | 用于荧光测量的激光扫描装置 |
US20110115895A1 (en) * | 2008-07-30 | 2011-05-19 | Jan Huisken | Multidirectional selective plane illumination microscopy |
US20120098949A1 (en) * | 2010-10-22 | 2012-04-26 | Leica Microsystems Cms Gmbh | Spim microscope with a sted light sheet |
CN104054014A (zh) * | 2011-10-28 | 2014-09-17 | 莱卡微系统Cms有限责任公司 | 用于以spim显微术照明样品的布置 |
CN103176268A (zh) * | 2011-12-21 | 2013-06-26 | 佳能株式会社 | 显微镜 |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111602080A (zh) * | 2017-12-07 | 2020-08-28 | 欧洲分子生物学实验室 | 用于成像多个样品的样品保持器 |
CN109765684A (zh) * | 2019-03-03 | 2019-05-17 | 北京工业大学 | 多光路多方位实时显微成像系统 |
CN109765684B (zh) * | 2019-03-03 | 2022-03-18 | 北京工业大学 | 多光路多方位实时显微成像系统 |
CN114787684A (zh) * | 2019-11-13 | 2022-07-22 | 华盛顿大学 | 具有照明及收集物镜的非正交布置的开顶式光片显微镜 |
Also Published As
Publication number | Publication date |
---|---|
EP3237949A2 (en) | 2017-11-01 |
WO2016105934A3 (en) | 2016-08-18 |
CN107003508B (zh) | 2020-07-10 |
US10481375B2 (en) | 2019-11-19 |
WO2016105934A2 (en) | 2016-06-30 |
EP3237949A4 (en) | 2018-08-01 |
JP2018506733A (ja) | 2018-03-08 |
JP6671725B2 (ja) | 2020-03-25 |
US20170371140A1 (en) | 2017-12-28 |
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CP03 | Change of name, title or address |
Address after: Massachusetts, USA Patentee after: Globegroup life technology consulting America Co.,Ltd. Address before: new jersey Patentee before: GE HEALTHCARE BIO-SCIENCES Corp. |
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TR01 | Transfer of patent right |
Effective date of registration: 20220104 Address after: Germany Wetzlar Patentee after: LEICA MICROSYSTEMS CMS GmbH Address before: Massachusetts Patentee before: Globegroup life technology consulting America Co.,Ltd. |
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TR01 | Transfer of patent right |