CN106982854A

CN106982854A - A kind of bactericidal composition

Info

Publication number: CN106982854A
Application number: CN201610034963.2A
Authority: CN
Inventors: 罗昌炎; 詹姆斯·T·布里斯托
Original assignee: Jiangsu Rotam Chemical Co Ltd
Current assignee: Jiangsu Rotam Chemical Co Ltd
Priority date: 2016-01-20
Filing date: 2016-01-20
Publication date: 2017-07-28

Abstract

The present invention relates to a kind of bactericidal composition；The invention further relates to the method for harmful fungoid is prevented and treated using described bactericidal composition.The microbicide compositions contain active component nitre benzene bacterium ester and Prochloraz, and the weight ratio of nitre benzene bacterium ester and Prochloraz is 10:1-1:50.The present invention also provides a kind of method for the plant pathogenic fungi for controlling plant, plant part, plant propagation material and the plant organ then grown.

Description

A kind of bactericidal composition

Technical field

The present invention relates to a kind of bactericidal composition；The invention further relates to prevent and treat harmful fungoid using described bactericidal composition Method.

Background technology

On pesticide activity, particularly to crop protection, one of key problem of research carried out in the technical field is Improve the performance in terms of performance, especially bioactivity and the performance in terms of keeping this activity within a certain period of time.

Have in the case of it is an object of the invention to provide being reduced in the administration total amount of reactive compound to harmful fungoid and change Enter the composition of activity（Synergistic composition）, to reduce the rate of application of known activity compound and improve its activity profile.

Nitre benzene bacterium ester（Meptyldinocap）；CAS:131-72-6；Chemical formula is：2,4- dinitros -6-（1- methyl heptan Base）Benzene crotonates；Molecular structural formula is：

Nitre benzene bacterium ester is the bactericide that Tao benefit agriculture is developed, and is the special efficacy of powdery mildew with protecting, treating and rooting out triple functions Medicament.

Prochloraz（Prochloraz）, its chemical name is：N- propyl group-N- [2- (2,4,6- Trichlorophenoxies) ethyl]- Imidazoles -1- formamides, are imidazoles wide-spectrum bactericides, and its structural formula is：

Prochloraz is worked by suppressing the biosynthesis of sterol, no systemic action, for caused by sac fungus and Fungi Imperfecti Multiple diseases preventive effect is splendid.Prochloraz uses gene inductive technology, and activated plant disease-resistant gene expression, quick-acting is good, the lasting period It is long.Prochloraz can be used for preventing and treating gourd, fruit and vegetable anthracnose, leaf spot, bakanae disease of rice, rice blast citrus anthracnose, blossom-end rot, Penicilliosis, green mould, banana anthracnose, leaf spot；Mango anthracnose, leaf spot of peanut, the vegetable such as capsicum, eggplant, muskmelon, tomato Dish anthracnose, Strawberry anthracnose, sclerotinia sclerotiorum, leaf spot, mushroom brown spot, apple anthracnose, pear scab etc..Prochloraz To there is treatment and eradicant action to the multiple diseases on field crop, fruit and vegetable, turf and ornamental plant.

Because the environmental requirement and economic requirement now to bactericide are constantly improve, such as to activity profile, toxicity, selection Property, the requirement of rate of application, residue composition and favourable preparation feasibility, additionally due to that may be deposited in terms of such as drug resistance In problem, therefore, exploitation is in some aspects lasting task better than the new bactericide of existing bactericide.

The content of the invention

And, it has been found that meanwhile, i.e., nitre benzene bacterium ester and Prochloraz are jointly or separately applied, or apply nitre benzene bacterium successively Ester and Prochloraz preferably to prevent and treat harmful fungoid than each compound is administered alone.

The invention provides a kind of bactericidal composition, said composition is answered by the way that nitre benzene bacterium ester and Prochloraz are carried out into binary Match somebody with somebody so that obtained mixture has gain effect in prevention effect, and has expanded fungicidal spectrum, effectively slows down or avoids disease Bacterium develops immunity to drugs.Astoundingly, the bactericidal activity of bactericidal composition of the invention is more active than each reactive compound Plus with it is considerably higher；There is unpredictable, necessary being cooperative effect, and be not only the supplement of activity.

When reactive compound with specific weight than be present in the present invention bactericidal composition in when, cooperative effect is especially bright It is aobvious.But, the weight ratio of the reactive compound in bactericidal composition of the present invention can change within the specific limits.

A kind of bactericidal composition of the present invention is to take following technical scheme to realize：

A kind of bactericidal composition, it is characterised in that：Containing active component nitre benzene bacterium ester and Prochloraz, wherein nitre benzene bacterium ester and miaow is fresh The percentage by weight of amine is 10:1-1:50, preferably 10:1-1:40, more preferably 10:1-1:30, it is further preferably 1:1- 1:25, it is further preferably 1:1-1:20, more preferably 1:1-1:15, more preferably 1:1-1:10, more preferably 1:1-1:5.

The weight proportion of nitre benzene bacterium ester and Prochloraz in the present invention for example can also be 10:1、5:1、1:1、1:5、1: 10、1:15、1:20、1:25、1:30、1:35、1:40、1:45、1:50。

Nitre benzene bacterium ester described in described bactericidal composition and Prochloraz quality sum account for the Bactericidal composite amount of substance 5%-90%, more preferably 10%-80%, more preferably 20%-60%.

Described Prochloraz includes Prochloraz metal salt compound.Wherein metal is first in the periodic table of elements Two transition elements, especially manganese, iron, zinc, copper；Also the second Main Group Metal Elements, from calcium or magnesium；And third and fourth Race's Main Group Metal Elements, especially aluminium or tin.

The Prochloraz metal salt compound is selected from prochloraz-manganese chloride complex, zinc salt, molysite, calcium salt, mantoquita, magnesium salts, aluminium salt And pink salt.It is preferred that prochloraz-manganese chloride complex.

Described bactericidal composition, also comprising filler and/or surfactant.

A kind of method for preventing and treating plant pathogen, pathogenic bacteria and/or its environment, Huo Zhezhi are acted on by bactericidal composition In thing, plant parts, seed, soil, material or space.

A kind of method for preventing and treating plant pathogen, nitre benzene bacterium ester and Prochloraz are administered simultaneously or applied respectively or successive Using.

A kind of bactericidal composition, includes nitre benzene bacterium ester and Prochloraz and filler and/or surfactant.

A kind of bactericidal composition, can be configured to any formulation agriculturally allowed.The formulation of described bactericidal composition is Suspending agent, seed coat agent, suspension emulsion, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, Missible oil, microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids.

Described bactericidal composition is used to preventing and treating Cereal, veterinary antibiotics, fungi and bacterium on ornamental plant and grapevine Purposes.

Described bactericidal composition is used to protect plant, plant part, plant propagation material and the plant device then grown The purposes of official.

Caused a disease or saprophytic in the place preventing and treating soil or cultivation medium of preventing and treating needed for described bactericidal composition is applied to Fungi and the purposes of bacterium.

Described bactericidal composition is used to protect the purposes of the plant or plant part after harvest.

Described bactericidal composition is used to protect stock in storage period from fungi or the purposes of bacterial invasion.

The bactericidal composition is used for the purposes for preventing and treating fruit vegetables storing period disease.

Described bactericidal composition is in various crop plants such as banana, cotton, vegetable variety（Such as cucumber, beans, tomato And cucurbitaceous plant）, it is barley, dogstail, oat, coffee, potato, corn, fruit variety, rice, rye, soybean, grapevine, small A large amount of fungies are prevented and treated in wheat, ornamental plant, sugarcane and a large amount of seeds to be even more important.

It is a kind of to control plant, plant part, the plant pathogenic fungi of plant propagation material and the plant organ then grown Method, including by the bactericidal composition of the present invention with agronomy is effective and amount of application of basic plant-less toxicity with seed treatment, The methods such as foliage applying, stem are applied, are impregnated with, instiling, pouring into a mould, spraying, spraying, dusting, distribution or smoke are administered to plant, plant Partly, plant propagation material or plant are being grown or needed in the soil or cultivation medium wherein grown.

The bactericidal composition of the present invention has very strong activity to various phytopathogens, and can be to being drawn by phytopathogen The prevention and treatment of the plant disease risen play very strong preventive effect.The present invention bactericidal composition to such as Plasmodiophoromycetes, Oomycete, Chytridiomycetes, connect true with the wide scope plant-pathogenic such as Gammaproteobacteria, Ascomycetes, Basidiomycetes, deuteromycetes, Phycomycetes Bacterium has excellent activity.

The bactericidal composition of the present invention also has extraordinary bactericidal performance, and it is thin to can be used for preventing and treating plant pathogenic Bacterium.Such as pseudomonadaceae, Rhizobiaceae, enterobacteriaceae, bar Cordycepps and Streptomycetaceae.

The bactericidal composition of the present invention can be used as blade face fungicide in crop protection, can act also as fungicide use In seed dressing and as soil fungicide.

The bactericidal composition of the present invention is particularly suitable for the following phytopathogen of preventing and treating：

As oomycetes subclass, can be exemplified as, such as various crops such as black leg of beet bacterium (Pythium ultium) corruption Mould category bacterium；Such as potato blight bacterium (PhytophthoraiNfestaNs), botrytis cinerea (Phytophthora Capsici Phytophthora category bacterium)；Such as bacterium of downy mildew of cucumber (PseudoperoNospora cubeNsis), Humulus The Pseudoperonospora bacterium of Pseudoperonospora cubensis (PseudoperoNospora humuli)；Such as Plasmopara viticola (Plasmopara Viticola Plasmopara bacterium)；Pseudoperonospora cubensis (PeroNospora brassicae), green onion downy mildew such as brassicaceous vegetable Germ (PeroNospora destructor), Peronospora bacterium of downy mildew of spinach bacterium (PeroNospora spiNaciae) etc..

As Ascomycetes, it can be exemplified as, for example, such as the powdery mildew of powdery mildew of cereals bacterium (ErysiphegramiNis) Belong to bacterium；Such as the Sphaerotheca bacterium of greengrocery powdery mildew (Sphaerotheca fuligiNea)；Such as apple black star bacteria (VeNturia iNaequalis), pear cucumerinum (VeNturia Nashicola) Venturia bacterium；Such as net blotch of barley The PyreNophora category bacterium of bacterium (PyreNophorateres)；Such as wheat class spot disease (Cochliobolus sativus) Cochliobolus belongs to bacterium；Such as Sclerotinia bacterium of greengrocery hyphal cluster germ (SclerotiNia sclerotiorum).

As Basidiomycetes, it can be exemplified as, for example, such as double spores of wheat leaf rust germ (PucciNiarecoNdita) Rust bacterium；Such as the Tilletia bacterium of bunt of wheat bacterium (Tilletia caries)；Such as loose smut of barley bacterium The Ustilago bacterium of (Ustilago Nuda) etc..

As Deuteromycotina, it can be exemplified as, for example, such as the stem point of lucid asparagus Phoma asparagi Sacc (Phomaasparagi) Mould category bacterium；Such as the Septoria bacterium of wheat class crust rot bacterium (Septoria Nodorum)；Such as Colletotrichumlagenarium The Colletotrichum bacterium of (Colletotrichum lageNarium)；Such as wilt (Pyricularia oryzae) Pyricularia belongs to bacterium；Such as the Botrytis bacterium of greengrocery ash arrhizus bacteria (Botrytis ciNerea)；Such as apple spot fallen leaves Germ (AlterNaria mali), the Alternaria bacterium of tomato early blight bacterium (AlterNaria solaNi)；Such as beet Cercospora The Cercospora bacterium of germ (Cercospora beticola)；Such as peach black star germ (Cladosporium carpophilum) Blastocladia bacterium；Such as Rhizoctonia bacterium of Oryza sheath blight fungus (RhizoctoNia solaNi).

The crop of the bactericidal composition processing of the present invention is such as but not limited to banana, cotton, vegetable variety（For example it is yellow Melon, beans, tomato and cucurbitaceous plant）, it is barley, dogstail, oat, coffee, potato, corn, fruit variety, rice, rye, big Beans, grapevine, wheat, ornamental plant, sugarcane and a large amount of seeds.

The crop mainly includes field crop, such as corn, soybean, cotton, rape oilseeds, such as southern general rape (Brassica napus) (such as rape (canola)), overgrown with weeds blue or green (Brassica rapa), leaf mustard (B.juncea) (such as mustard Sub (mustard)) and brassicacarinata (Brassica carinata), rice, wheat, beet, sugarcane, oat, brown wheat, greatly Wheat, broomcorn millet, triticale, flax, grapevine and the other fruit of various plants or vegetable crop, such as rose family (Rosaceaesp.) (for example, pip fruit, such as apple and pears, also drupe, such as apricot, cherry, almond and peach, berry such as strawberry), tea sugarcane Scarabaeidae (Ribesioidae sp.), Juglandaceae (Juglandaceae sp.), Betulaceae (Betulaceae sp.), Anacardiaceae (Anacardiaceae sp.), Fagaceae (Fagaceae sp.), Moraceae (Moraceae sp.), Oleaceae (Oleaceae Sp.), Actinidiaceae (Actinidaceae sp.), Lauraceae (Lauraceae sp.), Musaceae (Musaceae sp.) be (for example Banana tree and powder bajiao banana (plantings)), Rubiaceae (Rubiaceae sp.) (such as coffee), Theaceae (Theaceae Sp.), Sterculiaceae (Sterculiceaesp.), Rutaceae (Rutaceae sp.) (such as lemon, orange and grape fruit)；Solanaceae (Solanaceaesp.) (for example, tomato, potato, pepper, eggplant), Liliaceae (Liliaceae sp.), composite family (Compositiae sp.) (such as lettuce, globe artichoke and witloof-include root witloof (root chicory), hare's-lettuce (endive) Or common witloof (common chicory)), Umbelliferae (Umbelliferae sp.) (such as carrot, parsley, celery and block Rooted celery), Curcurbitaceae (Cucurbitaceae sp.) (for example cucumber-include pickled cucumber (pickling cucumber), south Melon, watermelon, cucurbit and muskmelon), green onion section (Alliaceae sp.) (such as onion and leek), Cruciferae (Cruciferae Sp.) (for example white cabbage, red cabbage, broccoli, cauliflower, brussels sprout, green vegetables, kohlrabi, radish, horseradish, solely Row dish, Chinese cabbage), pulse family (Leguminosaesp.) (such as peanut, pea and French beans-such as climning bean and broad bean), Chenopodiaceae (Chenopodiaceae sp.) (such as fodder beet, spinach sweet tea (spinach beet), spinach, beet root), Malvaceae (Malvaceae) (such as gumbo), Asparagaceae (Asparagaceae) (such as lucid asparagus)；Garden crop and forest crop； Ornamental plant；And the homologue of the genetic modification of these crops.

The bactericidal composition of the present invention can also be used for processing genetically modified organism, GMO (GMOs), such as plant or seed.Lose It is heterologous gene plant of the stable integration into genome to pass modified plant (or genetically modified plants).

Term " heterologous gene " is substantially such a gene, and the gene is provided or assembled outside plant, when the base Because be introduced into Matrix attachment region, Chloroplast gene or mitochondrial genomes when, by expressing protein interested or polypeptide or Person is obtained with new or improved agronomic quality by lowering other genes present in plant or making these gene silencings Or the conversion plant (using such as antisense technology, co-suppression technology or RNA interference-RNAi technology) of other properties.Positioned at gene Heterologous gene in group is also referred to as transgenosis.The transgenosis defined in Plant Genome by ad-hoc location is referred to as conversion or transgenosis Event.

The bactericidal composition of the present invention can also be used to treat the fungal disease that easily on timber or wood internal grows." art Language " timber refers to all types of timber and all types of rapidoprints for construction industry is processed to the timber, Such as solid wood, high density timber, pressed laminated wood and glued board.

The bactericidal composition of the present invention is particularly suitable for preventing and treating following plants disease fungus：

The two spore powdery mildews on standing grain powdery mildew (Erysiphe graminis), Curcurbitaceae on Cereal And the apple white powder on Siberian cocklebur monofilament shell (Sphaerotheca fuliginea), apple (Erysiphecichoracearum) On grape snag shell (Uncinula necator), Cereal on germ (Podosphaera leucotricha), grape The smut on rhizoctonia (Rhizoctonia), Cereal and sugarcane on handle rest fungus (Puccinia), cotton, rice and lawn (Ustilago) the compacted spore bacterium of length on venturia inaequalis (Venturia inaequalis), on apple, Cereal Glume blight bacterium (Septorianodorum), strawberry, vegetables, ornamental plant and Portugal on (Helminthospori um), wheat The peanut tail spore bacterium (Cercospora arachidicola) on botrytis cinerea (Botrytiscinera), peanut on grape, wheat With the eye spot bacterium (Pseudocercosporella herpotrichoides) on barley, the Magnaporthe grisea on rice On phytophthora infestans (Phytophthora infestans), grape on (Pyricularia oryzae), potato and tomato Grape life single shaft mould (Plasmoparaviticola), hops and Pseudocercosporella on cucumber, vegetables and Spherical cavity bacterium (Mycosphaerella) on alternaric bacteria (Alternaria) on fruit, banana, and sickle-like bacteria And verticillium sp (Verticillium) (Fusarium).

The bactericidal composition of the present invention is especially suitable for preventing and treating banana, cotton, vegetable variety（For example cucumber, beans, tomato and Cucurbitaceous plant）, barley, dogstail, oat, coffee, potato, corn, fruit variety, rice, rye, soybean, grapevine, wheat, Ornamental plant, sugarcane and downy mildew, early blight, late blight, balck shank, scab, leaf spot, anthrax on a large amount of seeds Disease, powdery mildew, wilt disease, shot hole, leaf rust.

The present invention provides a kind of method for preventing and treating plant pathogen, and bactericidal composition is acted on into pathogenic bacteria and/or its ring In border, or plant, plant parts, plant propagation material, soil, material or space.

The bactericidal composition of the present invention can be used for protection plant, plant part, plant propagation material and the plant then grown Sundries official.

The bactericidal composition of the present invention, can handle all plants and plant part." plant " refers to all plants and plant Population, such as preferably with undesirable wild plant, cultivated plant and plant variety (regardless of whether by plant variety or plant Cultivate the protection of people's right).Cultivated plant and plant variety can be the plants obtained by Sterile culture and breeding method, this A little methods can be aided with or be supplemented with one or more biological technique methods, for example, use dihaploid, protoplast fusion, random With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant Above and below the ground part and organ, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind Son and root, bulb and rhizome.Crop and nourish and generate and case of propagation material, for example, transplant, bulb, rhizome, running roots Plant part is fallen within seed.

Term " plant propagation material " is interpreted as referring to all plant parts for having a fertility, such as seed, and it can be used In breeding the latter, and vegetable matter such as cuttage bar or stem tuber（Such as potato）.Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.Treat The germination plant and effective plant suppressed after being germinateed from soil or after emerging.Young plant can be before transplantation by being impregnated into Row whole or Local treatment are protected.

Currently preferred propagating materials is seed.The bactericidal composition of the present invention is also particularly suitable for handling seed.Big portion Point harmful fungoid caused by crop infringement be due to during storing or after sowing and in plant germination process or send out Caused by the infringement of seed after bud.Because the root and branch of growth period plant are especially sensitive and also can even if small infringement Cause the death of plant.This stage is particularly critical, because protecting seed and germination by using suitable composition Plant is extremely important.

It is a problem studied for a long period of time that plant pathogenic fungi is controlled by handling the seed of plant.The present invention is another Aspect provides a kind of method for protecting seed and germination plant, and this method additionally apply after planting or after plant germination With crop protection agents or at least significantly additionally apply crop protection agents.On the other hand, the bactericidal composition using the present invention is excellent The amount of reactive compound used in changing, farthest to provide the protection of seed and germination plant against plant-pathogenic The invasion and attack of fungi, and plant in itself will not by damaged using reactive compound.

Therefore, the present invention is also more particularly to planted by using the composition of the present invention to handle seed with protecting seed and germinateing The method that thing is attacked from plant pathogenic fungi.The invention further relates to protected according to composition of the invention in processing seed Seed and purposes of the plant from plant pathogenic fungi of germinateing.Moreover, it relates to use the composition according to the present invention Handle to be protected from the seed of plant pathogenic fungi infringement.

After harm budding the control of the plant pathogenic fungi of plant it is main by using crop protection agents handle soil and The aerial part of plant is carried out.Healthy issuable influence in view of crop protection agents on environment and humans and animals, Therefore by the necessary amount of application for reducing reactive compound as far as possible.

It is suitable to protect in agricultural, in greenhouse, in forestry according to the bactericidal composition of the present invention or gardening-or vinegrowing Plant the seed for any plant variety applied.Especially, its seed form used is cereal（Such as wheat, barley, rye, black Wheat, millet, oat）, corn, cotton, soybean, paddy rice, potato, sunflower, Kidney bean, coffee, beet, peanut, rape, olive Olive, cocoa, sugarcane, tobacco, vegetables（Such as tomato, cucumber, onion and lettuce）, turfgrass and decoration use plant.Cereal and vegetable The processing of the seed of dish class is vital.

Active component nitre benzene bacterium ester and Prochloraz in the bactericidal composition of the present invention is individually or with suitable dosage form It is applied to seed.The processing preferably in the state of substantially stabilized is so that processing will not cause any infringement to seed.It is logical Often, random time point that can be between harvesting and sowing carries out processing seed.Commonly used seed from plant separate and Isolated from cob, shell, stem, epidermis, hair or pulp.Therefore, it is possible to use for example, being plucked, being cleaned and being dried to aqueous Seed of the amount less than 15 weight %.Alternatively, it is also possible to for example with water process after use is dry, the kind then dried once more Son.

The method of seed treatment, for example, can there are, the medicament of diluent liquid or solid-like or without dilution directly general Seed is immersed in the method for making medicament be impregnated with seed in liquid condition solution, is blended in solid chemicals or liquid preparation and seed Together, be coated processing make the surface of the seed adhere to medicament method, plantation while near seed sprinkling etc. method.

Plant part and the plant organ then grown are by any of the plant propagation material such as plant of seed generation Part.Plant part, plant organ and plant can also be benefited from by the way that bactericidal composition is applied into plant propagation material institute The pathogenic damage protection of acquisition.The plant organ grown behind certain plants part and some places can also regard plant propagation as Material, its own can be applied with bactericidal composition（Or processing）；So as to by the plant part through processing and the plant through processing Plant, other plant parts and the other plant organs that organ is produced can also be benefited from by the way that bactericidal composition is applied With.

The bactericidal composition of the present invention can also be used to prevent or control soil or cultivate a variety of in medium cause a disease or saprophytic Fungi and bacterium.The example of the aquacultural fungal pathogen of soil-borne includes Alternaria（Alternaria spp.）, Ascochyta (Ascochyta spp.), Botrytis cinerea（Botrytis cinerea, Cercospora（Cercospora spp., ergot （Clavicepspurpurea）, standing grain cochliobolus（Cochliobolussativus）, Colletotrichum（colletotrichum Spp., Epicoccum（Epicoccum spp., F.graminearum schw（Fusariumgraminearum）, rice evil seedling chain spore （Fusariummoniliforme）, fusarium oxysporum（Fusariumoxysporum, fusarium moniliforme （Fusariumproliferatum）, fusariun solani (Fusariumsolani), dimension glue chain spore (Fusariumsubglitinans), Helminthosporium (Helminthosporiumspp), the rotten microtorr bacterium of snow (Microdochiumnivale), Penicillium (Pencilliumspp), Phoma (Phoma spp.), wheat class nuclear cavity bacteria (Pyrenophoragraminea), rice blast Pyricularia Sacc. (Pyriculariaoryzae), Rhizoctonia solani Kuhn (Rhizoctoniasolani), Rhizoctonia cerealis (Rhizoctoniacerealis), Sclerotinia (Sclerotinia Spp.), Septoria (Septoria spp.), silk axle smut (Sphacelothecareilliana), Tilletia (Tilletia spp.), meat spore core coral bacterium (Typhula incarnate), hidden bar smut (Urocystisocculta) is black Powder Pseudomonas (Ustilago spp.) or Verticillium (Verticillium spp.).

Soil germ has rhizoctonia solani, sickle-like bacteria, phytophthora, bacterium of dampinging off, root rot, pythium spp, botrytis cinerea, soft-rot fungi Deng.In general, soil germ can produce a large amount of thalline, as long as condition is developed favourable to pathogen growth, host is sense Disease, germ just with amount reproduction and can infect host, in the presence of susceptible host, and these germs can just enter lasting The phase of causing a disease, with the continuous cropping of crop, amount reproduction is spread, but nutrient is depleted or edaphic condition such as temperature, humidity etc. afterwards When unfavorable to germ, germ can enter rest period again.When susceptible host is not present, soil-borne disease bacterium can also survive in soil Get off, in addition to soil germ has extensive host range, moreover it is possible to survived on the root surface of non-host or the broken branches and fallen leaves, with it It is undivided with saprophytic competitiveness.But different germs are discrepant, as sickle-like bacteria almost can be unlimited in soil Phase survives.

Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, for example：Soil, water etc., Specific raw material can use for example sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Wood chip, bark etc..

The method that medicament is applied into soil, for example, be diluted in liquid preparation in water or do not dilute and be directly applied to plant The medium method in the root of body or the rice seedling bed of seedling, granule is disseminated to the side in the rice seedling bed of the root of plant or seedling Method has the method that pulvis, water dispersible granules etc. are sprayed in soil prior to seeding and integrally mixed with soil, and sowing is preceding or plants It will be sprayed on before planting plant after pulvis, water-dispersible grain dilution agent in implantation hole, seed furrow, in method sowed etc..

The Fungicidai mixtures of the present invention can also be used to prevent and treat fruit vegetables storing period disease.The fruit for example as caused by following pathogen It is real to rot：

Aspergillus bacterium, such as aspergillus flavus；

Botrytis（Botrytis）Strain, such as Botrytis cinerea（Botrytis cinerea）;

Penicillium（Penicillium）Strain, such as penicillium expansum（Penicillium expansum）With P.purpurogenum;

Sclerotinia（Sclerotinia）Strain, such as sclerotinite（ Sclerotinia sclerotiorum）;

Verticillium（Verticilium）Strain, such as black and white wheel branch spore（Verticilium alboatrum）.

The bactericidal composition of the present invention be also particularly well suited for preventing and treating fruit vegetables storing period disease blossom-end rot, green mould, penicilliosis, Anthracnose.

Another object of the present invention controls plant, plant part, plant propagation material and then grown there is provided a kind of The method of the plant pathogenic fungi of plant organ, this method include by the present invention bactericidal composition with agronomy it is effective and substantially without Phytotoxic amount of application is applied with seed treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution Or the method such as smoke is administered to plant, plant part, plant propagation material or plant and is growing or needing what is grown wherein In soil or cultivation medium.

The bactericidal composition of the present invention can be applied by different processing methods, and these methods are for example：

- liquid comprising the bactericidal composition is sprayed onto to the aerial part of the plant；

- dusting, mixes particle or powder in soil, in surrounding plants sprinkling, and in tree injection or the situation of smearing Under；

- seed of plant is coated or film coated.

- when being used for postharvest fruit and vegetable anti-corrosive fresh-keeping, be generally diluted with water 200-2000 times of liquid, leached after leaching fruit.

The present invention provides a kind of method for preventing and treating plant pathogen, can be treatment, prevention or method of eradication.

After harvest can also being protected using the bactericidal composition according to the present invention when plant or plant part grow Plant or plant part.

According to the present invention, the disease after harvest with storage period can be for example by caused by following fungi：Colletotrichum kind, Such as banana thorn disk spore (Colletotrichum musae), Colletotrichum gloeosporiodes (Colletotrichum Gloeosporioides), capsicum thorn disk spore (Colletotrichum coccodes)；Fusarium kind, such as half-naked reaping hook Bacterium (Fusarium semitectum), fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium Solani), Fusarium oxysporum (Fusarium oxysporum)；Verticillium dahliae category kind, such as cocoa verticillium sp (Verticillium theobromae)；Nigrospora category kind；Botrytis kind, such as the pathogen of Botrytis cinerea (Botrytis cinerea)；Geotrichum, such as geotrichum candidum (Geotrichum candidum)；Phomopsis kind, Natal Phomopsis (Phomopsis natalensis)；Diplodia kind, such as spore of citrus color two (Diplodia citri)；Alternaria kind, example Such as Altemaria citri (Alternaria citri), mutually every Alternariaspp (Alternaria alternata)；Phytophthora kind, example Such as phytophthora brown rot of citrus (Phytophthora citrophthora), strawberry phytophthora (Phytophthora fragariae), dislike Phytophthora (Phytophthora cactorum), Phytophthora nicotianae (Phytophthora parasitica);Septoria (Septoria spp.), such as Septoria depressa；Mucor (Mucor spp.), such as pyriform Mucor (Mucor piriformis)；Raw chain sclerotinia sclerotiorum (the Monilinia of chain sclerotinia sclerotiorum belong (Monilinia spp.), such as fruit Fructigena), drupe chain sclerotinia sclerotiorum (Monilinia laxa)；Venturia (Venturia spp.), the black star of such as apple Bacterium (Venturia inaequalis), Pear scab (Venturia pyrina)；Rhizopus (Rhizopus spp.), for example Rhizopus stolonifer (Rhizopus stolonifer), Rhizopus oryzae (Rhizopus oryzae)；It is small to belong to (Glomerella from shell Spp.), for example enclose small from shell (Glomerella cingulata)；Sclerotinia (Sclerotinia spp.), such as fruit are raw Sclerotinite (Sclerotinia fruiticola)；Long beak shell belongs to (Ceratocystis spp.), such as unusual long beak shell (Ceratocystis paradoxa)；Penicillium (Penicillium spp.), such as penicillium funiculosum (Penicillium Funiculosum), penicillium expansum (Penicillium expansum), Penicillium digitatum (Penicillium digitatum), Italian mould (Penicillium italicum)；The long spore category (Gloeosporium spp.) of disk, such as white long spore of disk (Gloeosporium album), Gloeosporium perennans, the long spore (Gloeosporium of the raw disk of fruit fructigenum)、Gloeosporium singulata；Shell snake spore belongs to (Phlyctaena spp.), such as Phlyctaena vagabunda；Cylindrocarpon (Cylindrocarpon spp.), such as apple post spore (Cylindrocarpon mali)；Crawl handle Mould category (Stemphyllium spp.), such as day lily stemphylium botryosum (Stemphyllium vesicarium)；Star check shell spore Belong to (Phacydiopycnis spp.), such as Phacydiopycnis malirum；Thiclaviopsis (Thielaviopsis Spp.), such as singular root string strain mould (Thielaviopsis paradoxy)；Aspergillus (Aspergillus spp.) is such as black Aspergillus (Aspergillus niger), Aspergillus carbonerius (Aspergillus carbonarius);Nectria (Nectria Spp.), such as red shell bacterium (Nectria galligena) of dry cancer clump；Stockless Peziza (Pezicula spp.).

Treatment in accordance with the present invention method can also be used for the invasion and attack for protecting stock from fungi and microorganism.According to this hair It is bright, term " stock " is understood as referring to have originated from natural life cycle and it is desirable that the plant preserved for a long time or animality are come The natural materials in source and its processed form.The stock of plant origin, such as plant or part thereof, such as stem, leaf, stem tuber, Seed, fruit or seed, can be with the state of fresh harvesting or with form processing such as (pre-) dry, wetting, crushing, grinding, pressure System or baking are protected.Can also be timber, thick timber form such as construction timber, electric pole and fence；Or final product form, such as by Furniture or article that timber is made.The stock of animal origin is animal skin, leather, hair, hair etc..According to the composition of the present invention It can prevent the invasion and attack such as burn into of the fungi or bacterium of storage period from fading or mouldy.It is preferred that " stock " is understood as referring to plant The natural materials and its form processing in thing source, more preferably fruit and its form processing, such as the operatic circle, drupe, soft fruit and Citrus fruit and its form processing.

The bactericidal composition of the present invention can be made into common dosage form, such as missible oil, wettable powder, suspending agent, liquid The dosage forms such as agent, granule, seed coat agent are used, its amount of application, according to the mixing ratio, meteorological condition, medicament of active ingredient Form, Dressing date, application process, using place, prevent and kill off the different and variant of object noxious livings, objective crop etc..

There may be super plus (" collaboration ") effect for treatment in accordance with the present invention.For example, the sterilization group used according to the present invention The rate of application of compound and/or widen its field of activity and/or increase its activity, it is possible to obtain following effect：More preferable plant Growth, the tolerance increase to high temperature or low temperature, to arid or the increase of the tolerance of water or soil salt content, performance of blooming is carried It is high, it is easier to harvest, the maturation of quickening, higher yield rate, bigger fruit, higher plant height, the color of leaf is more It is green, bloom earlier, the quality or nutritive value of the product of harvest are higher, sugared concentration is higher in fruit, the storage of the product of harvest More preferably, these benefits have exceeded the effect actually estimated for stability and/or processability.

The present invention processing method can be additionally used in handle propagating materials such as stem tuber or rhizome, and available for handle seed, Seedling or transplanting (pricking out) seedling and plant or transplanting plant.The processing method can also be used for handling root.The present invention Processing method can also be used for handling the aerial part of plant such as about the doing of plant, stem or stalk, leaf, flower and fruit.

- generally for leaf portion processing：0.1-10000g/ha, preferably 10-1000 g/ha, more preferably 50-500 g/ha；It is right For dipping or administration of instiling, the dosage can also even be reduced, particularly when using inert base such as asbestos or pearl During rock；

- for seed treatment：2-5000g/100kg seeds, preferably 3-1000g/100kg seeds；

- for soil or processing used for ponds：0.1-10000g/ha, preferably 1-1000g/ha.

- fresh-keeping for postharvest fruit and vegetable, leached after 200-2000 times of liquid, leaching fruit can be diluted.

Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to actual feelings during actual administration Condition and needs, the seriousness especially in accordance with the property and germ of pending plant or crop adjust rate of application.

The nitre benzene bacterium ester of the present invention combines/combined administration with Prochloraz.Including separating, sequentially or simultaneously applying nitre benzene bacterium ester With Prochloraz.Preferably, the nitre benzene bacterium ester is combined as the form of the composition comprising nitre benzene bacterium ester and Prochloraz with Prochloraz.

The present invention composition can be based on dosage form, i.e., each material is blended in composition, composition into Point can also unit dose form provide, mixed using preceding in bucket or tank, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.

As a further improvement on the present invention, bactericidal composition of the invention can be configured to any agent agriculturally allowed Type.

As a further improvement on the present invention, the formulation of bactericidal composition of the invention is suspending agent, seed coat agent, wettable It is pulvis, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, super Low capacity liquor, suspension emulsion.

In bactericidal composition of the present invention, nitre benzene bacterium ester and Prochloraz, filler and/or surfactant are included.

Bactericidal composition of the present invention, the content of wherein nitre benzene bacterium ester and Prochloraz accounts for the 5%- of bactericidal composition 90%。

Described bactericidal composition, the content of wherein nitre benzene bacterium ester and Prochloraz accounts for the 10%-80% of bactericidal composition.

Described bactericidal composition, the content of wherein nitre benzene bacterium ester and Prochloraz accounts for the 20%-60% of bactericidal composition.

According to the present invention, term " filler ", which refers to, can be combined with reactive compound or combines to make it easier for being administered to Object（Such as plant, crop or careless class）Natural or synthetic organic or inorganic compound.Therefore, the filler is preferably It is inert, it at least should be agricultural acceptable.The filler can be solid or liquid.

The nonactive medium that can be used in the present invention both can be that solid can also be liquid, can be used as solid matchmaker What dielectric material was used has for example：Phyteral powdery type（Such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut Shell powder, wheat bran, cellulose powder, coconut husk, the particle of corncob and tobacco stem, extract residue after plant essence etc.）, paper , sawdust, crush synthesized polymer body, the clay class of synthetic resin etc.（Such as kaolin, bentonite, acid china clay）, talcum powder Class.Silica（Such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates）, activated carbon, natural mineral matter class（Float stone, Lv Po Thread stone and zeolite etc.）, fire diatomite, sand, plastics medium etc.（Such as polyethylene, polypropylene, polyvinylidene chloride）, chlorination Chemical fertilizer, the soil of inorganic mineral powder, ammonium sulfate, ammonium phosphate, urea, the ammonium chloride of potassium, calcium carbonate, calcium phosphate etc. etc. Fertilizer, these materials can be used alone or two or more is mixed.

Can be selected in llowing group of materials as liquid media materials'use, such as water, alcohol type（Such as first Alcohol, ethanol, isopropanol, butanol, ethylene glycol etc.）, ketone（Such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone Deng）, ethers（Such as ether, dioxanes, methylcellulose, tetrahydrofuran）, aliphatic hydrocarbon class（Such as kerosene, Mineral oil etc.）, aromatic hydrocarbons class（Such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro fat Fat hydrocarbon, chlorobenzene, etc.）, halogenated hydrocarbon class, amide-type, sulfone class, dimethyl sulfoxide (DMSO), mineral and vegetable oil, animal oil etc..

To make the emulsification of active ingredient compound, it is scattered and/or soak, can use surfactant for example can be with Enumerate AEO, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the fatty acid ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total to Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as thing, alkyltaurate, protein hydrolysate.Suitable oligosaccharide thing or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or its with for example（It is polynary）Alcohol or（It is polynary）The combination of amine.

To make active ingredient compound dispersion stability, attachment and/or combining, such as xanthans, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid（Such as cephalin and lecithin）With And the adjuvant such as synthetic phospholipid, bentonite, sodium lignin sulfonate.

The wherein optional spent glycol of antifreezing agent, propane diols, glycerine, sorbierite.It is used as the deflocculant of suspension product The adjuvant such as naphthalene sulfonic acid polymer, polymeric phosphate can be used.

Organic silicon defoamer can be used as defoamer.

The colouring agent that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff：Alizarin dyes, azo dyes and metallized phthalocyanine dye；And trace element, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.

Optionally, can also include other annexing ingredients, for example protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.

The reactive compound can be mixed and prepared by the preparation of the present invention by known way with conventional additives. The conventional additives such as conventional extender and solvent or diluent, emulsifying agent, dispersant, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, drier and colouring agent, stabilizer, pigment, defoamer, preservative, increasing can also be included Thick dose, water and other processing aids.

These compositions not only include can be by suitable equipment as sprayed or dusting equipment is immediately suitable for pending Object, and it is additionally included in the concentration commercial composition for needing to be diluted before being applied to object.

The bacterium ester of benzene containing nitre and Prochloraz of the present invention can also be applied with other active ingredient combinations, such as expanding work Property spectrum or prevent from forming resistance.Other active components such as fungicide, bactericide, attractant, insecticide, mite killing Agent, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..

Reactive compound nitre benzene bacterium ester and Prochloraz can be administered simultaneously, or apply respectively, or sequential application, during separate administration Order on the result of preventing and treating generally without influence.

The bactericidal composition of the present invention can be based on dosage form, i.e., each material is blended in composition, composition Composition can also unit dose form provide, mixed using preceding in bucket or tank, be then diluted to required concentration.Wherein preferably with Based on the dosage form that the present invention is provided.

Although single reactive compound shows weak Fungicidally active, the combination product, which has, exceedes activity simply Plus the activity of sum.The summation of reactive compound activity when the Fungicidally active of active compound product, which exceedes, to be administered alone When, the synergy of fungicide is constantly present.

The bactericidal composition of the present invention is applied under total amount in reduction reactive compound, and having to harmful fungoid improves activity （Synergistic）.And the bactericidal composition of the present invention shows that the bacterium of tolerance also has excellent kill to existing bactericide Bacterium effect.

A kind of bactericidal composition of the present invention, said composition by nitre benzene bacterium ester and Prochloraz by carrying out binary built so that Obtained composition has gain effect in prevention effect, and has expanded fungicidal spectrum, serves the multi-purpose effect of a medicine, has Effect slows down or avoids germ from developing immunity to drugs.Astoundingly, the bactericidal activity of bactericidal composition of the invention is than each activity The active of compound adds and considerably higher, there is unpredictable, necessary being cooperative effect, and is not only activity Supplement.When reactive compound with specific weight than be present in the present invention bactericidal composition in when, cooperative effect is particularly evident. But, the weight ratio of the reactive compound in bactericidal composition of the present invention can change within the specific limits.

Can have greater activity and the more long bactericidal composition of activity holding the invention provides a kind of.The sterilization group Compound has relatively low-dose, while the toxicity of the bactericidal composition is relatively low, to the Cereal and fruits and vegetables with fungal disease and Its seed is controlled.

Can have greater activity and the more long bactericidal composition of activity holding the invention provides a kind of.

The bactericidal composition of the present invention plays Synergistic, suitable for preventing and treating harmful fungoid.And the sterilization of the present invention Composition is particularly suitable for the powdery mildew fungi in preventing and treating Cereal, veterinary antibiotics, ornamental plant and grapevine.

Embodiment

First passing through specific example of formulations below, the invention will be further elaborated.

Example of formulations

The Prochloraz wettable powder of 1 10% nitre benzene bacterium ester of embodiment+50%

Nitre benzene bacterium ester 10%

Prochloraz 50%

Lauryl sodium sulfate 10%

Sodium lignin sulfonate 5%

White carbon 10%

Kaolin complements to 100%

Active component, various auxiliary agents and filler etc. are mixed by the proportional components of formula, after ultra-fine pulverizer disintegrating, that is, obtained The Prochloraz wettable powder of 10% nitre benzene bacterium ester+50%.

The prochloraz-manganese chloride complex wettable powder of 2 5% nitre benzene bacterium ester of embodiment+20%

Nitre benzene bacterium ester 5%

Prochloraz-manganese chloride complex 20%

Calcium dodecyl benzene sulfonate 1%

Sodium lignin sulfonate 2%

Sucrose complements to 100%

Active component, various auxiliary agents and filler etc. are mixed by the proportional components of formula, after ultra-fine pulverizer disintegrating, that is, obtained The prochloraz-manganese chloride complex wettable powder of 5% nitre benzene bacterium ester+20%.

The Prochloraz missible oil of 3 20% nitre benzene bacterium ester of embodiment+40%

Nitre benzene bacterium ester 20%

Prochloraz 40%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 complement to 100%

Mentioned component is proportionally prepared, homogeneous phase is uniformly mixing to obtain.

The prochloraz-manganese chloride complex wettable powder of 4 5% nitre benzene bacterium ester of embodiment+25%

Nitre benzene bacterium ester 5%

Prochloraz-manganese chloride complex 25%

Lauryl sodium sulfate 10%

Sodium lignin sulfonate 5%

White carbon 10%

Kaolin complements to 100%

Said components are mixed in proportion, and grinds, crush, wettable powder is prepared into.

The prochloraz-manganese chloride complex water dispersible granules of 5 10% nitre benzene bacterium ester of embodiment+20%

Nitre benzene bacterium ester 10%

Prochloraz-manganese chloride complex 20%

MODIFIED LIGNOSULPHONATE 5%

Lauryl sodium sulfate 5%

Urea 5%

Kaolin complements to 100%

Prochloraz active component, dispersant, wetting agent, disintegrant and filler are well mixed in the ratio of formula, by air-flow It is ground into wettable powder；Nitre benzene bacterium ester is added to be well mixed；Add a certain amount of water mixing extruding and make material.Through dry sieve The prochloraz-manganese chloride complex water dispersible granules of 10% nitre benzene bacterium ester+20% are obtained after point.

The Prochloraz suspension emulsion of 6 10% nitre benzene bacterium ester of embodiment+25%

Oil phase：

Nitre benzene bacterium ester 10%

Methyl oleate 10%

Ethoxylated castor oil 5%

Aqueous phase：

Prochloraz 25%

The sodium salt 1% of naphthalene sulfonic acids-formaldehyde condensation products of sulfonation

Water complements to 100%

Nitre benzene bacterium ester is dissolved in methyl oleate, ethoxylated castor oil is added and obtains oil phase；According to formula by Prochloraz, sulphur Sodium salt, the water of naphthalene sulfonic acids-formaldehyde condensation products of change is ground and/or high speed shear after obtain the aqueous suspension agent of Prochloraz； Oil phase addition aqueous phase is obtained into suspension emulsion under stirring.

The Prochloraz wettable powder of 7 10% nitre benzene bacterium ester of embodiment+10%

Nitre benzene bacterium ester 10%

Prochloraz 10%

Sodium lignin sulfonate 1%

NaLS 2%

The silicic acid 1% of high degree of dispersion

Kaolin complements to 100%

The Prochloraz coated granule of 8 10% nitre benzene bacterium ester of embodiment+80%

Nitre benzene bacterium ester 10%

Prochloraz 80%

Polyethylene glycol 3%

The silicic acid 1% of high degree of dispersion

Calcium carbonate complements to 100%

In a mixer, by levigate active component even spread to the carrier soaked by polyethylene glycol.It can obtain in this way Obtain dust-free coated granules agent.

The Prochloraz missible oil of 9 15% nitre benzene bacterium ester of embodiment+25%

Nitre benzene bacterium ester 15%

Prochloraz 25%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 complement to 100%

Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.

The Prochloraz microemulsion of 10 20% nitre benzene bacterium ester of embodiment+5%

Nitre benzene bacterium ester 20%

Prochloraz 5%

Ethoxylated castor oil 15%

Calcium dodecyl benzene sulfonate 10%

SOLVESSO^TM200 complement to 100%

The Prochloraz of 11 20% nitre benzene bacterium ester of embodiment+60% extrudes granule

Nitre benzene bacterium ester 20%

Prochloraz 60%

Sodium lignin sulfonate 4%

Carboxymethyl cellulose 2%

Kaolin complements to 100%

Active component is mixed and ground with auxiliary agent, mixture is soaked with water.The mixture is extruded, then done in the air stream It is dry.

The Prochloraz suspension seed-coating agent of 12 5% nitre benzene bacterium ester of embodiment+5%

Nitre benzene bacterium ester 5%

Prochloraz 5%

AEO sulfosuccinic acid monoesters disodium 10%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

PVP-K30 1%

Water complements to 100%

Suspension seed-coating agent is obtained after above-mentioned each component is mixed into ground and/or high speed shear in proportion.

The Prochloraz microcapsule suspending agent of 13 5% nitre benzene bacterium ester of embodiment+30%

ATLOX^TM4913 2%

Citric acid 0.05%

Catalyst 0.1%

Water 10%

Prochloraz 30%

PAPI 1.35%

SOLVESSO^TM200 5%

ATLOX^TM4913 10%

Dispersant LFH 0.3%

Defoamer 0.16%

Urea 10%

Nitre benzene bacterium ester 5%

Water complements to 100%

By poly methylene poly phenyl poly isocyanate（PAPI）, nitre benzene bacterium ester, SOLVESSO^TM200 oil phases formed, which are added, to be contained ATLOX^TMIn 4913 aqueous solution, emulsion is formed.Then heat and be incubated 50^oCatalyst reaction is added under C 2 hours. The microcapsules of nitre benzene bacterium ester is obtained after cooling.

ATLOX^TM4913, dispersant LFH, defoamer, urea, Prochloraz and water are mixed in proportion, it is ground and/or Obtain uniform after high speed shear, obtain suspending agent.

In the suspending agent that obtained nitre benzene bacterium ester micro capsule agent is added to Prochloraz, 5% nitre benzene bacterium ester+30% is uniformly mixing to obtain Prochloraz microcapsule suspension suspending agent.

The Prochloraz suspension emulsion of 14 5% nitre benzene bacterium ester of embodiment+20%

Nitre benzene bacterium ester 5%

Prochloraz 20%

SOLVESSO^TM200 10%

Ethoxylated castor oil 4%

AEO sulfosuccinic acid monoesters disodium 5%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

Water complements to 100%

Nitre benzene bacterium ester is dissolved in SOLVESSO^TMIn 200, ethoxylated castor oil is added, the oil phase of nitre benzene bacterium ester is obtained；

AEO sulfosuccinic acid monoesters disodium, MODIFIED LIGNOSULPHONATE, Prochloraz and water are mixed in proportion Close, obtain uniform after ground and/or high speed shear, obtain the suspending agent of Prochloraz.

The oil phase of the bacterium ester of benzene containing nitre is added to the aqueous suspension agent containing Prochloraz, suspension emulsion is obtained.

The Prochloraz missible oil of 15 25% nitre benzene bacterium ester of embodiment+25%

Nitre benzene bacterium ester 25%

Prochloraz 25%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 complement to 100%

The prochloraz-manganese chloride complex water dispersible granules of 16 5% nitre benzene bacterium ester of embodiment+30%

Nitre benzene bacterium ester 5%

Prochloraz-manganese chloride complex 30%

MODIFIED LIGNOSULPHONATE 5%

Lauryl sodium sulfate 5%

Urea 5%

Kaolin complements to 100%

Nitre benzene bacterium ester, prochloraz-manganese chloride complex active component, dispersant, wetting agent, disintegrant and filler are mixed in the ratio of formula Uniformly, by air-flow crushing into wettable powder；Add a certain amount of water mixing extruding and make material.5% is obtained after drying screening The prochloraz-manganese chloride complex water dispersible granules of nitre benzene bacterium ester+30%.

The prochloraz-manganese chloride complex wettable powder of 17 10% nitre benzene bacterium ester of embodiment+40%

Nitre benzene bacterium ester 10%

Prochloraz-manganese chloride complex 40%

Lauryl sodium sulfate 1%

Sodium lignin sulfonate 1%

Kaolin complements to 100%

The Prochloraz of 18 20% nitre benzene bacterium ester of embodiment+80%

Nitre benzene bacterium ester 20%

Prochloraz 80%

Nitre benzene bacterium ester, Prochloraz are proportionally well mixed.

The prochloraz-manganese chloride complex of 19 10% nitre benzene bacterium ester of embodiment+90%

Nitre benzene bacterium ester 10%

Prochloraz-manganese chloride complex 90%

Nitre benzene bacterium ester, prochloraz-manganese chloride complex are proportionally well mixed.

Proportioning in above example is weight per distribution ratio.

Biological test example：

Agricultural chemicals is made in the combination of active principles of different agricultural chemicals, is current exploitation and develops novel pesticide and prevent and treat agriculturally resistance A kind of effective and quick way of germ.After the pestsides synthesis of different cultivars, three kinds of type of action are typically exhibited：It is added and makees With, synergistic effect and antagonism.But it is specially which kind of effect, it is impossible to predict, only just can know that by many experiments.Compounding Synergy is formulated well, due to significantly improving actual prevention effect, reduces the usage amount of agricultural chemicals, so as to greatly delay The generation of resistance.

First, virulence is tested：

The toxicity index of each medicament and the co-toxicity coefficient of mixture are calculated according to the abundant methods of Sun Yun（CTC values）, when CTC≤80, then combine Thing shows antagonism, when 80<CTC<120, then composition show summation action, when CTC >=120, then composition is showed Go out synergistic effect.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

B in A percentage composition+B medicament toxicity index * mixtures in theoretical toxicity index (TTI)=A medicament toxicity index * mixtures Percentage composition

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI) * 100

Phytopathogen and host plant used in greenhouse virulence test

Host plant	Disease title	Genic organisms
			Wheat	Powdery mildew	Erysiphe graminis f.sptritici
Wheat	Leaf rust	Puccinia triticinia
			Apple	Shot hole	Apple black star bacteria
Grape	Powdery mildew	Grape powdery mildew
			Cucumber	Powdery mildew	Two spore powdery mildews
Cucumber	Downy mildew	Bacterium of downy mildew of cucumber
			Citrus	Penicilliosis	Penicillium notatum

Experiment 1：The toxicity test of powdery mildew of cucumber

Selected from the consistent cucumber seedling of growing way, sprayed with potter spray towers under 50PSI pressure, per basin about 5mL, each medicine Agent sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the powdery mildew of cucumber leaf in field uniform above cucumber seedling Shake off conidium to be inoculated with, then cucumber seedling is put into greenhouse and cultivated.It is classified after 7d according to the morbidity of powdery mildew of cucumber Standard complete stool investigates disease index, and calculates prevention effect, and concentration EC50 in suppressing then is calculated with least square method, then according to grandson Yun Peifa calculates co-toxicity coefficient (CTC).

Virulence test result of the present invention of table 1 on powdery mildew of cucumber is prevented and treated

As known from Table 1, nitre benzene bacterium ester and Prochloraz are preventing the weight proportion 10 of powdery mildew of cucumber:1-1:When in 50 scope, Co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 2：The toxicity test of wheat leaf rust

Selected from the consistent wheat seedling of growing way, each processing supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the wheat in field Leaf rust leaf is uniformly shaken off conidium above wheat seedling and is inoculated with, and then wheat seedling is put into greenhouse and cultivated.After 7d Disease index is investigated according to the morbidity grade scale complete stool of wheat leaf rust, and calculates prevention effect, least square method is then used Concentration EC50 in suppressing is calculated, then co-toxicity coefficient (CTC) is calculated according to the abundant methods of Sun Yun.

Table 2：Virulence test result of the present invention on preventing and treating wheat leaf rust

As known from Table 2, nitre benzene bacterium ester and Prochloraz are preventing the weight proportion 10 of wheat leaf rust:1-1:When in 50 scope, Co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 3：The toxicity test of uncinula necator

Six weeks big reservation two panels of selection completely open the mattae of leaf.Sprayed with potter spray towers under 50PSI pressure Mist, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will have been infected grape white powder The sick leaf of disease is uniformly shaken off conidium on mattae and is inoculated with, and then mattae is put into greenhouse and cultivated.7d Disease index is investigated according to the morbidity grade scale complete stool of uncinula necator afterwards, and calculates prevention effect, least square is then used Method calculates concentration EC50 in suppression, then calculates co-toxicity coefficient (CTC) according to the abundant methods of Sun Yun.

Virulence test result of the present invention of table 3 on prevention uncinula necator

As known from Table 3, nitre benzene bacterium ester and Prochloraz are preventing the weight proportion 10 of uncinula necator:1-1:When in 50 scope, Co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 4：The toxicity test of wheat powdery mildew

Selected from the consistent wheat seedling of growing way, sprayed with potter spray towers under 50PSI pressure, per basin about 5mL, each medicine Agent sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the wheat powdery mildew leaf in field uniform above wheat seedling Shake off conidium to be inoculated with, then wheat seedling is put into greenhouse and cultivated.It is classified after 7d according to the morbidity of wheat powdery mildew Standard complete stool investigates disease index, and calculates prevention effect, and concentration EC50 in suppressing then is calculated with least square method, then according to grandson Yun Peifa calculates co-toxicity coefficient (CTC).

Virulence test result of the present invention of table 4 on preventing and treating wheat powdery mildew

As known from Table 4, nitre benzene bacterium ester and prochloraz-manganese chloride complex are preventing the weight proportion 10 of wheat powdery mildew:1-1:In 50 scope When, co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 5：The toxicity test of apple scab

Apple seedling is selected, is sprayed with potter spray towers under 50PSI pressure, per basin about 5mL, each medicament sets 12 Individual concentration gradient.24h connects bacterium after chemicals treatment, will pick up from the sick leaf that is infected by apple scab and uniformly shakes off on apple seedling Conidium is inoculated with, and then apple seedling is put into greenhouse and cultivated.It is classified and marks according to the morbidity of apple scab after 7d Quasi- complete stool investigates disease index, and calculates prevention effect, and concentration EC50 in suppressing then is calculated with least square method, then according to Sun Yun Abundant method calculates co-toxicity coefficient (CTC).

Virulence test result of the present invention of table 5 on apple scab is prevented and treated

As known from Table 5, nitre benzene bacterium ester and Prochloraz are preventing the weight proportion 10 of apple scab:1-1:When in 50 scope, Co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 6：The toxicity test of Penicillium italicum

Using suppression mycelial growth rate method：Experiment target is designated as Penicillium italicum.

Nitre benzene bacterium ester and Prochloraz are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution The decoction of series concentration, draws 6mL to the conical flask of sterilizing, the potato of 50 DEG C or so of addition respectively in superclean bench Glucose agar medium（PDA）54mL, pours into 4 diameter 9cm plate after shaking up, the toxic training of 4 respective concentrations is made Support base；Toxic culture medium is made in the nitre benzene bacterium ester and Prochloraz series concentration compound solution of different ratio with same method. The Penicillium italicum bacterium of 2 days will be cultivated, break into fungus block in colony edge with diameter 5mm card punch, moved fungus block with transfer needle To the toxic PDA culture medium center being configured in advance, culture in 25 DEG C of incubators is subsequently placed in, often processing is repeated 4 times.After 3 days, Each processing colony diameter cm is measured with slide calliper rule using crossing method, correction is obtained and suppresses percentage.Each bacterium colony right-angled intersection Two diameters are surveyed, bacterium colony size is represented with its average.Then colony growth inhibiting rate is obtained as the following formula：

Then concentration EC in suppressing is calculated with least square method₅₀, then according to the abundant method calculating co-toxicity coefficients of Sun Yun（CTC）.

Table 6：To the virulence test result of Penicillium italicum

As known from Table 6, the combination preventing and treating Penicillium italicum of nitre benzene bacterium ester and Prochloraz is in proportioning 10:1-1:When in 50 scope, Co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 7：The toxicity test of cucumber downy mildew

Selected from the consistent cucumber seedling of growing way, sprayed with potter spray towers under 50PSI pressure, per basin about 5mL, each medicine Agent sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the cucumber downy mildew leaf in field uniform above cucumber seedling Shake off conidium to be inoculated with, then cucumber seedling is put into greenhouse and cultivated.It is classified after 7d according to the morbidity of cucumber downy mildew Standard complete stool investigates disease index, and calculates prevention effect, and concentration EC50 in suppressing then is calculated with least square method, then according to grandson Yun Peifa calculates co-toxicity coefficient (CTC).

Virulence test result of the present invention of table 7 on preventing and treating cucumber downy mildew

As known from Table 7, nitre benzene bacterium ester and Prochloraz are preventing the weight proportion 10 of cucumber downy mildew:1-1:When in 50 scope, Co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Claims

1. a kind of bactericidal composition, it is characterised in that：The bactericidal composition contains active component nitre benzene bacterium ester and Prochloraz, nitre The weight ratio of benzene bacterium ester and Prochloraz is 10:1-1:50, preferably 10:1-1:40, more preferably 10:1-1:30, then it is excellent Elect 1 as:1-1:25, it is further preferably 1:1-1:20, more preferably 1:1-1:15, more preferably 1:1-1:10.

2. bactericidal composition according to claim 1, it is characterised in that：The nitre benzene bacterium ester and Prochloraz quality sum are accounted for The 5%-90% of the Bactericidal composite amount of substance, more preferably more preferably 10%-80%, 20%-60%.

3. bactericidal composition according to claim 1, it is characterised in that：Prochloraz includes Prochloraz metallic salt chemical combination Thing, preferably prochloraz-manganese chloride complex.

4. bactericidal composition according to claim 1, it is characterised in that：The formulation of the bactericidal composition be suspending agent, Seed coat agent, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, missible oil, microemulsion, water Emulsion, effervescent tablet, ultra low volume liquids, suspension emulsion.

5. bactericidal composition according to claim 1, it is characterised in that：Also include filler and/or surfactant.

6. a kind of method for preventing and treating plant pathogen, it is characterised in that：Bactericidal composition described in claim 1 is acted on into cause In germ and/or its environment, or plant, seed, soil, material or space.

7. method according to claim 6, it is characterised in that：Nitre benzene bacterium ester and Prochloraz described in claim 1 is same When apply or apply respectively or sequential application.

8. the bactericidal composition described in claim 1 is used for the purposes for preventing and treating fungi and bacterium.

9. the bactericidal composition described in claim 1 is used to prevent and treat true on Cereal, veterinary antibiotics, ornamental plant and grapevine Bacterium and the purposes of bacterium.

10. the bactericidal composition described in claim 1 is used to protect plant, plant part, plant propagation material and then grow Plant organ purposes.

Caused a disease in the place preventing and treating soil or cultivation medium of preventing and treating needed for 11. the bactericidal composition described in claim 1 is applied to Or saprophytic fungi and the purposes of bacterium.

12. the bactericidal composition described in claim 1 is used to protect the purposes of the plant or plant part after harvest.

13. the bactericidal composition described in claim 1 is used to protect stock in storage period from fungi or the use of bacterial invasion On the way.

14. a kind of control plant, plant part, the plant pathogenic fungi of plant propagation material and the plant organ then grown Method, it is characterised in that：This method is included the bactericidal composition described in claim 1 with agronomy effectively and substantially without plant poison The amount of application of property is applied with seed treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution or smoke Etc. method be administered to plant, plant part, plant propagation material or plant growing or needing the soil that grows wherein or Cultivate in medium.